AU2017378990B2 - Polymerizing enzymes for sequencing reactions - Google Patents

Polymerizing enzymes for sequencing reactions Download PDF

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Publication number
AU2017378990B2
AU2017378990B2 AU2017378990A AU2017378990A AU2017378990B2 AU 2017378990 B2 AU2017378990 B2 AU 2017378990B2 AU 2017378990 A AU2017378990 A AU 2017378990A AU 2017378990 A AU2017378990 A AU 2017378990A AU 2017378990 B2 AU2017378990 B2 AU 2017378990B2
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Australia
Prior art keywords
seq
amino acids
polymerizing enzyme
amino acid
polymerase
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AU2017378990A
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AU2017378990A1 (en
Inventor
Mohammad Wadud BHUIYA
Thomas CHRISTIAN
Jeremy Lackey
Manjula PANDEY
Brian Reed
Jonathan M. Rothberg
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Quantum Si Inc
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Quantum Si Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AU2017378990A 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions Active AU2017378990B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662436410P 2016-12-19 2016-12-19
US62/436,410 2016-12-19
PCT/US2017/067427 WO2018118997A2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Publications (2)

Publication Number Publication Date
AU2017378990A1 AU2017378990A1 (en) 2019-06-20
AU2017378990B2 true AU2017378990B2 (en) 2024-05-16

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AU2017378990A Active AU2017378990B2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Country Status (11)

Country Link
US (2) US11312944B2 (enExample)
EP (1) EP3555272A2 (enExample)
JP (2) JP2020501573A (enExample)
KR (2) KR20230052995A (enExample)
CN (1) CN110268055A (enExample)
AU (1) AU2017378990B2 (enExample)
BR (1) BR112019012181A2 (enExample)
CA (1) CA3047321A1 (enExample)
MX (1) MX2019007248A (enExample)
TW (1) TWI788318B (enExample)
WO (1) WO2018118997A2 (enExample)

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WO2018204810A1 (en) 2017-05-05 2018-11-08 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
US10865441B2 (en) * 2019-03-13 2020-12-15 Abclonal Science, Inc. Mutant Taq polymerase for faster amplification
SG11202112620TA (en) * 2019-05-17 2021-12-30 4basebio Sl Phi29 dna polymerase mutants with improved primer recognition
MX2022000055A (es) * 2019-06-28 2022-05-30 Quantum Si Inc Enzimas de polimerización para reacciones de secuenciación.
AU2020364058A1 (en) 2019-10-11 2022-05-26 Quantum-Si Incorporated Surface modification in the vapor phase
CN115362266A (zh) * 2020-02-10 2022-11-18 铂赛基因组学公司 Phi29突变体及其用途
JP2023554368A (ja) 2020-12-15 2023-12-27 クアンタム－エスアイ インコーポレイテッド 単一分子速度論による分子バーコード分析
CN116083393B (zh) * 2023-02-14 2025-09-23 南方科技大学 一种dna装配分子机器及其应用
WO2025067248A1 (zh) * 2023-09-25 2025-04-03 成都今是科技有限公司 用于单分子测序的核酸聚合酶及其突变株
CN119082075B (zh) * 2024-09-12 2025-06-27 湖南艾科瑞生物工程有限公司 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用

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Also Published As

Publication number Publication date
BR112019012181A2 (pt) 2019-11-05
CA3047321A1 (en) 2018-06-28
JP2020501573A (ja) 2020-01-23
EP3555272A2 (en) 2019-10-23
US20230058852A1 (en) 2023-02-23
WO2018118997A2 (en) 2018-06-28
CN110268055A (zh) 2019-09-20
KR20190093659A (ko) 2019-08-09
US20180208911A1 (en) 2018-07-26
KR20230052995A (ko) 2023-04-20
TWI788318B (zh) 2023-01-01
JP2023026651A (ja) 2023-02-24
WO2018118997A3 (en) 2018-08-02
US20180362941A9 (en) 2018-12-20
MX2019007248A (es) 2019-08-16
US11312944B2 (en) 2022-04-26
TW201835328A (zh) 2018-10-01
AU2017378990A1 (en) 2019-06-20

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