TWI788318B - 用於定序反應之聚合酶 - Google Patents

用於定序反應之聚合酶 Download PDF

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Publication number
TWI788318B
TWI788318B TW106144668A TW106144668A TWI788318B TW I788318 B TWI788318 B TW I788318B TW 106144668 A TW106144668 A TW 106144668A TW 106144668 A TW106144668 A TW 106144668A TW I788318 B TWI788318 B TW I788318B
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Taiwan
Prior art keywords
polymerase
amino acids
seq
sequence
amino acid
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TW106144668A
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English (en)
Chinese (zh)
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TW201835328A (zh
Inventor
強納森 M 羅斯伯格
布萊恩 瑞德
莫漢麥德 瓦度德 布灰亞
曼朱拉 潘迪
湯瑪士 克里斯汀
傑瑞米 雷克伊
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美商寬騰矽公司
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Publication of TW201835328A publication Critical patent/TW201835328A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
TW106144668A 2016-12-19 2017-12-19 用於定序反應之聚合酶 TWI788318B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662436410P 2016-12-19 2016-12-19
US62/436,410 2016-12-19

Publications (2)

Publication Number Publication Date
TW201835328A TW201835328A (zh) 2018-10-01
TWI788318B true TWI788318B (zh) 2023-01-01

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TW106144668A TWI788318B (zh) 2016-12-19 2017-12-19 用於定序反應之聚合酶

Country Status (11)

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US (2) US11312944B2 (enExample)
EP (1) EP3555272A2 (enExample)
JP (2) JP2020501573A (enExample)
KR (2) KR20190093659A (enExample)
CN (1) CN110268055A (enExample)
AU (1) AU2017378990B2 (enExample)
BR (1) BR112019012181A2 (enExample)
CA (1) CA3047321A1 (enExample)
MX (1) MX2019007248A (enExample)
TW (1) TWI788318B (enExample)
WO (1) WO2018118997A2 (enExample)

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US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN113597468B (zh) * 2019-03-13 2024-09-13 武汉爱博泰克生物科技有限公司 用于快速扩增的突变型Taq聚合酶
CA3140244A1 (en) * 2019-05-17 2020-11-26 4basebio Sl Phi29 dna polymerase mutants with improved primer recognition
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CN114829669B (zh) 2019-10-11 2024-10-01 宽腾矽公司 气相表面改性
EP4103745A4 (en) * 2020-02-10 2024-03-13 Bioskryb Genomics, Inc. Phi29 mutants and use thereof
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CN116083393B (zh) * 2023-02-14 2025-09-23 南方科技大学 一种dna装配分子机器及其应用
EP4786584A1 (en) * 2023-09-25 2026-08-05 Geneus Technologies (Chengdu) Co., Ltd. Nucleic acid polymerase for single-molecule sequencing and mutant thereof
CN119082075B (zh) * 2024-09-12 2025-06-27 湖南艾科瑞生物工程有限公司 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用

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Also Published As

Publication number Publication date
KR20190093659A (ko) 2019-08-09
JP2020501573A (ja) 2020-01-23
MX2019007248A (es) 2019-08-16
KR20230052995A (ko) 2023-04-20
TW201835328A (zh) 2018-10-01
US12545901B2 (en) 2026-02-10
EP3555272A2 (en) 2019-10-23
US20180208911A1 (en) 2018-07-26
CA3047321A1 (en) 2018-06-28
US20230058852A1 (en) 2023-02-23
WO2018118997A2 (en) 2018-06-28
WO2018118997A3 (en) 2018-08-02
AU2017378990A1 (en) 2019-06-20
JP2023026651A (ja) 2023-02-24
CN110268055A (zh) 2019-09-20
US11312944B2 (en) 2022-04-26
BR112019012181A2 (pt) 2019-11-05
US20180362941A9 (en) 2018-12-20
AU2017378990B2 (en) 2024-05-16

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