BR112019012181A2 - enzimas polimerizantes para reações de sequenciamento - Google Patents

enzimas polimerizantes para reações de sequenciamento

Info

Publication number
BR112019012181A2
BR112019012181A2 BR112019012181-1A BR112019012181A BR112019012181A2 BR 112019012181 A2 BR112019012181 A2 BR 112019012181A2 BR 112019012181 A BR112019012181 A BR 112019012181A BR 112019012181 A2 BR112019012181 A2 BR 112019012181A2
Authority
BR
Brazil
Prior art keywords
polymerizing enzymes
sequencing reactions
nucleic acid
enzymes
polymerizing
Prior art date
Application number
BR112019012181-1A
Other languages
English (en)
Inventor
M. Rothberg Jonathan
Reed Brian
Wadud Bhuiya Mohammad
Pandey Manjula
Christian Thomas
Lackey Jeremy
Original Assignee
Quantum-Si Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum-Si Incorporated filed Critical Quantum-Si Incorporated
Publication of BR112019012181A2 publication Critical patent/BR112019012181A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

a presente invenção refere-se às composições compreendendo enzimas polimerizantes recombinantes modificadas, que são fornecidas, juntamente com a moléculas de ácido nucleico que codificam as enzimas de polimerização modificadas. em alguns aspectos, os métodos de uso de tais enzimas polimerizantes para sintetizar uma molécula de ácido nucleico ou sequenciar um padrão de ácido nucleico são fornecidos.
BR112019012181-1A 2016-12-19 2017-12-19 enzimas polimerizantes para reações de sequenciamento BR112019012181A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662436410P 2016-12-19 2016-12-19
PCT/US2017/067427 WO2018118997A2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Publications (1)

Publication Number Publication Date
BR112019012181A2 true BR112019012181A2 (pt) 2019-11-05

Family

ID=61148468

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019012181-1A BR112019012181A2 (pt) 2016-12-19 2017-12-19 enzimas polimerizantes para reações de sequenciamento

Country Status (11)

Country Link
US (2) US11312944B2 (pt)
EP (1) EP3555272A2 (pt)
JP (2) JP2020501573A (pt)
KR (2) KR20190093659A (pt)
CN (1) CN110268055A (pt)
AU (1) AU2017378990B2 (pt)
BR (1) BR112019012181A2 (pt)
CA (1) CA3047321A1 (pt)
MX (1) MX2019007248A (pt)
TW (1) TWI788318B (pt)
WO (1) WO2018118997A2 (pt)

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US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN113597468A (zh) * 2019-03-13 2021-11-02 武汉爱博泰克生物科技有限公司 用于快速扩增的突变型Taq聚合酶
AU2020277641A1 (en) * 2019-05-17 2021-12-09 4basebio Sl Phi29 DNA polymerase mutants with improved primer recognition
CN114302961A (zh) * 2019-06-28 2022-04-08 宽腾矽公司 用于测序反应的聚合酶
JP2022551523A (ja) 2019-10-11 2022-12-09 クアンタム－エスアイ インコーポレイテッド 気相における表面修飾
CN115362266A (zh) * 2020-02-10 2022-11-18 铂赛基因组学公司 Phi29突变体及其用途

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Also Published As

Publication number Publication date
TWI788318B (zh) 2023-01-01
WO2018118997A2 (en) 2018-06-28
AU2017378990A1 (en) 2019-06-20
AU2017378990B2 (en) 2024-05-16
JP2020501573A (ja) 2020-01-23
US20230058852A1 (en) 2023-02-23
CN110268055A (zh) 2019-09-20
WO2018118997A3 (en) 2018-08-02
KR20190093659A (ko) 2019-08-09
JP2023026651A (ja) 2023-02-24
EP3555272A2 (en) 2019-10-23
US11312944B2 (en) 2022-04-26
TW201835328A (zh) 2018-10-01
CA3047321A1 (en) 2018-06-28
KR20230052995A (ko) 2023-04-20
MX2019007248A (es) 2019-08-16
US20180208911A1 (en) 2018-07-26
US20180362941A9 (en) 2018-12-20

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B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
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