MX2019007248A - Enzimas de polimerizacion para reacciones de secuenciacion. - Google Patents

Enzimas de polimerizacion para reacciones de secuenciacion.

Info

Publication number
MX2019007248A
MX2019007248A MX2019007248A MX2019007248A MX2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A
Authority
MX
Mexico
Prior art keywords
polymerizing enzymes
sequencing reactions
nucleic acid
enzymes
polymerizing
Prior art date
Application number
MX2019007248A
Other languages
English (en)
Inventor
Reed Brian
Wadud Bhuiya Mohammad
Lackey Jeremy
M Rothberg Jonathan
Pandey Manjula
Christian Thomas
Original Assignee
Quantum Si Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantum Si Inc filed Critical Quantum Si Inc
Publication of MX2019007248A publication Critical patent/MX2019007248A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Las composiciones que comprenden las enzimas de polimerizacion recombinantes modificadas se proporcionan, junto con moleculas de acido nucleico que codifican las enzimas de polimerizacion modificadas; en algunos aspectos, se proporcionan los metodos para utilizar dichas enzimas de polimerizacion para sintetizar una molecula de acido nucleico o para secuenciar una plantilla de acido nucleico.
MX2019007248A 2016-12-19 2017-12-19 Enzimas de polimerizacion para reacciones de secuenciacion. MX2019007248A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662436410P 2016-12-19 2016-12-19
PCT/US2017/067427 WO2018118997A2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

Publications (1)

Publication Number Publication Date
MX2019007248A true MX2019007248A (es) 2019-08-16

Family

ID=61148468

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019007248A MX2019007248A (es) 2016-12-19 2017-12-19 Enzimas de polimerizacion para reacciones de secuenciacion.

Country Status (11)

Country Link
US (2) US11312944B2 (es)
EP (1) EP3555272A2 (es)
JP (2) JP2020501573A (es)
KR (2) KR20230052995A (es)
CN (1) CN110268055A (es)
AU (1) AU2017378990B2 (es)
BR (1) BR112019012181A2 (es)
CA (1) CA3047321A1 (es)
MX (1) MX2019007248A (es)
TW (1) TWI788318B (es)
WO (1) WO2018118997A2 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112019012414A2 (pt) 2016-12-19 2020-02-27 Quantum-Si Incorporated Carregamento de moléculas em poços de amostra para análise
EP3555272A2 (en) * 2016-12-19 2019-10-23 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
MX2019013111A (es) 2017-05-05 2019-12-16 Quantum Si Inc Sustratos que tienen reactividad de superficie modificada y propiedades antiincrustantes en reacciones biologicas.
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN113597468B (zh) * 2019-03-13 2024-09-13 武汉爱博泰克生物科技有限公司 用于快速扩增的突变型Taq聚合酶
JP2022533366A (ja) * 2019-05-17 2022-07-22 ４ベースバイオ ソシエダー リミターダ プライマー認識が改良されたＰｈｉ２９ ＤＮＡポリメラーゼ変異体
CA3145246A1 (en) * 2019-06-28 2020-12-30 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
EP4028570A1 (en) 2019-10-11 2022-07-20 Quantum-Si Incorporated Surface modification in the vapor phase
AU2021219665A1 (en) * 2020-02-10 2022-09-01 BioSkryb Genomics, Inc. Phi29 mutants and use thereof
CN116083393A (zh) * 2023-02-14 2023-05-09 南方科技大学 一种dna装配分子机器及其应用

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4435728A1 (de) 1994-01-19 1995-07-20 Boehringer Mannheim Gmbh Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
GB2333597B (en) 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
DE19810879A1 (de) * 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
US6153442A (en) 1998-05-20 2000-11-28 Dade Behring Inc. Reagents and methods for specific binding assays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AU772281B2 (en) 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6914128B1 (en) 1999-03-25 2005-07-05 Abbott Gmbh & Co. Kg Human antibodies that bind human IL-12 and methods for producing
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
MXPA03009566A (es) 2001-04-19 2004-12-06 Scripps Research Inst Metodos y composicion para la produccion de pares trna-aminoaciltrna sintetasa ortogonales.
WO2004092331A2 (en) 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US8637650B2 (en) 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
DE602006016984D1 (de) 2005-12-12 2010-10-28 Us Gov Health & Human Serv Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren
EP1963536B1 (en) * 2005-12-22 2016-05-04 Pacific Biosciences of California, Inc. Polymerases for nucleotide analogue incorporation
US8084734B2 (en) 2006-05-26 2011-12-27 The George Washington University Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
CA2718404C (en) 2008-03-13 2018-04-10 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
EP2274449B1 (en) 2008-03-28 2015-12-23 Pacific Biosciences of California, Inc. Methods for nucleic acid sequencing
CA2720247C (en) 2008-03-31 2020-07-14 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2942404B1 (en) 2008-03-31 2016-11-23 Pacific Biosciences of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
JP2012507986A (ja) 2008-11-03 2012-04-05 カパバイオシステムズ キメラｄｎａポリメラーゼ
WO2010059206A2 (en) 2008-11-19 2010-05-27 Pacific Biosciences Of California, Inc. Modular nucleotide compositions and uses therefor
WO2010065322A1 (en) 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
KR101063981B1 (ko) 2009-01-12 2011-09-14 서강대학교산학협력단 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법
DE102009013653B4 (de) 2009-03-18 2014-09-18 Bruker Daltonik Gmbh Protein-Sequenzierung mit MALDI-Massenspektrometrie
WO2010111686A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Labeled enzyme compositions, methods & systems
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
EP2414545B1 (en) 2009-04-03 2017-01-11 Sequenom, Inc. Nucleic acid preparation compositions and methods
ES2359058B1 (es) * 2009-07-02 2012-03-27 Consejo Superior De Investigaciones Cient�?Ficas (Csic) Quimera de adn polimerasa del fago ph1 29.
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
WO2011040971A2 (en) * 2009-09-30 2011-04-07 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
SG10201503540QA (en) 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US8906660B2 (en) * 2012-02-01 2014-12-09 Pacific Biosciences Of California, Inc. Recombinant polymerases with increased phototolerance
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
US9435810B2 (en) 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
CN105308073B (zh) 2013-06-14 2019-08-13 加利福尼亚太平洋生物科学股份有限公司 双生物素化标签
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
EP3030683B1 (en) 2013-08-05 2022-03-02 Pacific Biosciences of California, Inc. Protected fluorescent reagent compounds
CA3208970A1 (en) 2014-09-15 2016-05-06 Board Of Regents, The University Of Texas System Improved single molecule peptide sequencing
WO2016126941A1 (en) 2015-02-04 2016-08-11 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
CA3040566A1 (en) 2015-10-16 2017-04-20 The Governing Council Of The University Of Toronto Protein sequencing methods and reagents
US10814299B2 (en) 2015-11-18 2020-10-27 Pacific Biosciences Of California, Inc. Loading nucleic acids onto substrates
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
EP3555272A2 (en) * 2016-12-19 2019-10-23 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
JP7418323B2 (ja) 2017-08-24 2024-01-19 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 遠赤および近ｉｒ範囲におけるシアニンフルオロフォアの配座の束縛
US10836798B2 (en) 2018-11-08 2020-11-17 Government Of The United States Of America, As Represented By The Secretary Of Commerce Amino acid-specific binder and selectively identifying an amino acid

Also Published As

Publication number Publication date
US20180362941A9 (en) 2018-12-20
US20180208911A1 (en) 2018-07-26
US11312944B2 (en) 2022-04-26
BR112019012181A2 (pt) 2019-11-05
JP2023026651A (ja) 2023-02-24
EP3555272A2 (en) 2019-10-23
CA3047321A1 (en) 2018-06-28
CN110268055A (zh) 2019-09-20
TW201835328A (zh) 2018-10-01
WO2018118997A3 (en) 2018-08-02
AU2017378990B2 (en) 2024-05-16
AU2017378990A1 (en) 2019-06-20
KR20230052995A (ko) 2023-04-20
TWI788318B (zh) 2023-01-01
WO2018118997A2 (en) 2018-06-28
US20230058852A1 (en) 2023-02-23
KR20190093659A (ko) 2019-08-09
JP2020501573A (ja) 2020-01-23

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