JP2020501573A - シークエンシング反応用の重合酵素 - Google Patents

シークエンシング反応用の重合酵素 Download PDF

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Publication number
JP2020501573A
JP2020501573A JP2019533058A JP2019533058A JP2020501573A JP 2020501573 A JP2020501573 A JP 2020501573A JP 2019533058 A JP2019533058 A JP 2019533058A JP 2019533058 A JP2019533058 A JP 2019533058A JP 2020501573 A JP2020501573 A JP 2020501573A
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polymerase
amino acid
recombinant
sequence
region
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JP2019533058A
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JP2020501573A5 (enExample
Inventor
エム． ロスバーグ、ジョナサン
エム． ロスバーグ、ジョナサン
リード、ブライアン
ワダッド ブイヤ、モハマド
ワダッド ブイヤ、モハマド
パンデー、マンジュラ
クリスチャン、トーマス
ラッキー、ジェレミー
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Quantum Si Inc
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Quantum Si Inc
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Publication of JP2020501573A publication Critical patent/JP2020501573A/ja
Publication of JP2020501573A5 publication Critical patent/JP2020501573A5/ja
Priority to JP2023000897A priority Critical patent/JP2023026651A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2019533058A 2016-12-19 2017-12-19 シークエンシング反応用の重合酵素 Pending JP2020501573A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023000897A JP2023026651A (ja) 2016-12-19 2023-01-06 シークエンシング反応用の重合酵素

Applications Claiming Priority (3)

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US201662436410P 2016-12-19 2016-12-19
US62/436,410 2016-12-19
PCT/US2017/067427 WO2018118997A2 (en) 2016-12-19 2017-12-19 Polymerizing enzymes for sequencing reactions

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JP2023000897A Division JP2023026651A (ja) 2016-12-19 2023-01-06 シークエンシング反応用の重合酵素

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JP2020501573A true JP2020501573A (ja) 2020-01-23
JP2020501573A5 JP2020501573A5 (enExample) 2021-02-04

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JP2023000897A Pending JP2023026651A (ja) 2016-12-19 2023-01-06 シークエンシング反応用の重合酵素

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US (2) US11312944B2 (enExample)
EP (1) EP3555272A2 (enExample)
JP (2) JP2020501573A (enExample)
KR (2) KR20190093659A (enExample)
CN (1) CN110268055A (enExample)
AU (1) AU2017378990B2 (enExample)
BR (1) BR112019012181A2 (enExample)
CA (1) CA3047321A1 (enExample)
MX (1) MX2019007248A (enExample)
TW (1) TWI788318B (enExample)
WO (1) WO2018118997A2 (enExample)

Cited By (1)

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JP2023026651A (ja) * 2016-12-19 2023-02-24 クアンタム－エスアイ インコーポレイテッド シークエンシング反応用の重合酵素

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JP7356909B2 (ja) 2017-05-05 2023-10-05 クアンタム－エスアイ インコーポレイテッド 生物学的反応における表面反応性と防汚特性が改変された基板
CA3089756A1 (en) 2018-01-29 2019-08-01 St. Jude Children's Research Hospital, Inc. Method for nucleic acid amplification
US10934533B1 (en) 2018-10-10 2021-03-02 New England Biolabs, Inc. Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA
CN113597468B (zh) * 2019-03-13 2024-09-13 武汉爱博泰克生物科技有限公司 用于快速扩增的突变型Taq聚合酶
CA3140244A1 (en) * 2019-05-17 2020-11-26 4basebio Sl Phi29 dna polymerase mutants with improved primer recognition
KR20220029708A (ko) * 2019-06-28 2022-03-08 퀀텀-에스아이 인코포레이티드 서열분석 반응을 위한 중합 효소
CN114829669B (zh) 2019-10-11 2024-10-01 宽腾矽公司 气相表面改性
EP4103745A4 (en) * 2020-02-10 2024-03-13 Bioskryb Genomics, Inc. Phi29 mutants and use thereof
AU2021401791A1 (en) 2020-12-15 2023-07-06 Quantum-Si Incorporated Molecular barcode analysis by single-molecule kinetics
CN116083393B (zh) * 2023-02-14 2025-09-23 南方科技大学 一种dna装配分子机器及其应用
EP4786584A1 (en) * 2023-09-25 2026-08-05 Geneus Technologies (Chengdu) Co., Ltd. Nucleic acid polymerase for single-molecule sequencing and mutant thereof
CN119082075B (zh) * 2024-09-12 2025-06-27 湖南艾科瑞生物工程有限公司 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用

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Publication number Priority date Publication date Assignee Title
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KR20190093659A (ko) 2019-08-09
MX2019007248A (es) 2019-08-16
KR20230052995A (ko) 2023-04-20
TW201835328A (zh) 2018-10-01
US12545901B2 (en) 2026-02-10
EP3555272A2 (en) 2019-10-23
US20180208911A1 (en) 2018-07-26
CA3047321A1 (en) 2018-06-28
US20230058852A1 (en) 2023-02-23
WO2018118997A2 (en) 2018-06-28
WO2018118997A3 (en) 2018-08-02
AU2017378990A1 (en) 2019-06-20
JP2023026651A (ja) 2023-02-24
CN110268055A (zh) 2019-09-20
US11312944B2 (en) 2022-04-26
BR112019012181A2 (pt) 2019-11-05
TWI788318B (zh) 2023-01-01
US20180362941A9 (en) 2018-12-20
AU2017378990B2 (en) 2024-05-16

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