MX2019007248A - Enzimas de polimerizacion para reacciones de secuenciacion. - Google Patents
Enzimas de polimerizacion para reacciones de secuenciacion.Info
- Publication number
- MX2019007248A MX2019007248A MX2019007248A MX2019007248A MX2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A MX 2019007248 A MX2019007248 A MX 2019007248A
- Authority
- MX
- Mexico
- Prior art keywords
- sequencing reactions
- polymerizing enzymes
- nucleic acid
- enzymes
- polymerization enzymes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662436410P | 2016-12-19 | 2016-12-19 | |
| PCT/US2017/067427 WO2018118997A2 (en) | 2016-12-19 | 2017-12-19 | Polymerizing enzymes for sequencing reactions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2019007248A true MX2019007248A (es) | 2019-08-16 |
Family
ID=61148468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019007248A MX2019007248A (es) | 2016-12-19 | 2017-12-19 | Enzimas de polimerizacion para reacciones de secuenciacion. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11312944B2 (enExample) |
| EP (1) | EP3555272A2 (enExample) |
| JP (2) | JP2020501573A (enExample) |
| KR (2) | KR20190093659A (enExample) |
| CN (1) | CN110268055A (enExample) |
| AU (1) | AU2017378990B2 (enExample) |
| BR (1) | BR112019012181A2 (enExample) |
| CA (1) | CA3047321A1 (enExample) |
| MX (1) | MX2019007248A (enExample) |
| TW (1) | TWI788318B (enExample) |
| WO (1) | WO2018118997A2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3555314B1 (en) | 2016-12-19 | 2023-08-16 | Quantum-Si Incorporated | Loading molecules into sample wells for analysis |
| WO2018118997A2 (en) * | 2016-12-19 | 2018-06-28 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| CA3059211A1 (en) | 2017-05-05 | 2018-11-08 | Quantum-Si Incorporated | Substrates having modified surface reactivity and antifouling properties in biological reactions |
| IL276343B2 (en) | 2018-01-29 | 2024-10-01 | St Jude Childrens Res Hospital Inc | Method for nucleic acid amplification |
| US10934533B1 (en) | 2018-10-10 | 2021-03-02 | New England Biolabs, Inc. | Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA |
| CN113597468B (zh) * | 2019-03-13 | 2024-09-13 | 武汉爱博泰克生物科技有限公司 | 用于快速扩增的突变型Taq聚合酶 |
| JP2022533366A (ja) * | 2019-05-17 | 2022-07-22 | ４ベースバイオ ソシエダー リミターダ | プライマー認識が改良されたＰｈｉ２９ ＤＮＡポリメラーゼ変異体 |
| EP3976771A1 (en) * | 2019-06-28 | 2022-04-06 | Quantum-si Incorporated | Polymerizing enzymes for sequencing reactions |
| BR112022006682A2 (pt) | 2019-10-11 | 2022-07-12 | Quantum Si Inc | Modificação de superfície na fase de vapor |
| EP4103745A4 (en) * | 2020-02-10 | 2024-03-13 | Bioskryb Genomics, Inc. | Phi29 mutants and use thereof |
| AU2021401791A1 (en) | 2020-12-15 | 2023-07-06 | Quantum-Si Incorporated | Molecular barcode analysis by single-molecule kinetics |
| CN116083393B (zh) * | 2023-02-14 | 2025-09-23 | 南方科技大学 | 一种dna装配分子机器及其应用 |
| WO2025067248A1 (zh) * | 2023-09-25 | 2025-04-03 | 成都今是科技有限公司 | 用于单分子测序的核酸聚合酶及其突变株 |
| CN119082075B (zh) * | 2024-09-12 | 2025-06-27 | 湖南艾科瑞生物工程有限公司 | 性能提升的phi29 DNA聚合酶突变体及其制备方法与应用 |
Family Cites Families (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4435728A1 (de) | 1994-01-19 | 1995-07-20 | Boehringer Mannheim Gmbh | Biotinsilan-Verbindungen und diese Verbindungen enthaltende Bindematrix |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| GB2333597B (en) | 1996-10-29 | 2001-04-18 | Univ Nebraska At Lincoln | Method for detecting point mutations in dna utilizing fluorescence energy transfer |
| DE19810879A1 (de) | 1998-03-13 | 1999-09-16 | Roche Diagnostics Gmbh | Polymerasenchimären |
| US6153442A (en) | 1998-05-20 | 2000-11-28 | Dade Behring Inc. | Reagents and methods for specific binding assays |
| US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| JP4638043B2 (ja) | 1998-12-14 | 2011-02-23 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法 |
| US6914128B1 (en) | 1999-03-25 | 2005-07-05 | Abbott Gmbh & Co. Kg | Human antibodies that bind human IL-12 and methods for producing |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| US6869764B2 (en) | 2000-06-07 | 2005-03-22 | L--Cor, Inc. | Nucleic acid sequencing using charge-switch nucleotides |
| US6936702B2 (en) | 2000-06-07 | 2005-08-30 | Li-Cor, Inc. | Charge-switch nucleotides |
| CA2314398A1 (en) | 2000-08-10 | 2002-02-10 | Edward Shipwash | Microarrays and microsystems for amino acid analysis and protein sequencing |
| IL158418A0 (en) | 2001-04-19 | 2004-05-12 | Scripps Research Inst | In vivo incorporation of unnatural amino acids |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| US20100035254A1 (en) | 2003-04-08 | 2010-02-11 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| GB0324456D0 (en) | 2003-10-20 | 2003-11-19 | Isis Innovation | Parallel DNA sequencing methods |
| WO2005044836A2 (de) | 2003-11-05 | 2005-05-19 | Genovoxx Gmbh | Makromolekulare nukleotidverbindungen und methoden zu deren anwendung |
| US7462452B2 (en) | 2004-04-30 | 2008-12-09 | Pacific Biosciences Of California, Inc. | Field-switch sequencing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| ATE481505T1 (de) | 2005-12-12 | 2010-10-15 | Us Gov Health & Human Serv | Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren |
| CA2633524A1 (en) * | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Polymerases for nucleotide analogue incorporation |
| US8084734B2 (en) | 2006-05-26 | 2011-12-27 | The George Washington University | Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays |
| US8617811B2 (en) | 2008-01-28 | 2013-12-31 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
| WO2009114182A1 (en) | 2008-03-13 | 2009-09-17 | Pacific Biosciences Of California, Inc. | Labeled reactants and their uses |
| EP3269824A1 (en) | 2008-03-28 | 2018-01-17 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
| US8906831B2 (en) | 2008-03-31 | 2014-12-09 | Pacific Biosciences Of California, Inc. | Single molecule loading methods and compositions |
| US8999676B2 (en) | 2008-03-31 | 2015-04-07 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for improved single molecule sequencing |
| US8257954B2 (en) | 2008-03-31 | 2012-09-04 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| US9127259B2 (en) * | 2008-03-31 | 2015-09-08 | Pacific Biosciences Of California, Inc. | Enzymes resistant to photodamage |
| US8420366B2 (en) | 2008-03-31 | 2013-04-16 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| EP2352818B1 (en) | 2008-11-03 | 2016-02-10 | Kapa Biosystems, Inc. | Chimeric dna polymerases |
| US8252910B2 (en) | 2008-11-19 | 2012-08-28 | Pacific Biosciences Of California, Inc. | Modular nucleotide compositions and uses therefor |
| CA2745197A1 (en) | 2008-12-01 | 2010-06-10 | Research Triangle Institute | Concurrent identification of multitudes of polypeptides |
| KR101063981B1 (ko) | 2009-01-12 | 2011-09-14 | 서강대학교산학협력단 | 자유 라디칼 개시제 및 이를 이용한 펩타이드 서열의 동정방법 |
| DE102009013653B4 (de) | 2009-03-18 | 2014-09-18 | Bruker Daltonik Gmbh | Protein-Sequenzierung mit MALDI-Massenspektrometrie |
| WO2010111691A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corp | Conjugates of biomolecules to nanoparticles |
| WO2010117420A2 (en) | 2009-03-30 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Fret-labeled compounds and uses therefor |
| EP2414545B1 (en) | 2009-04-03 | 2017-01-11 | Sequenom, Inc. | Nucleic acid preparation compositions and methods |
| ES2359058B1 (es) * | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
| US9566335B1 (en) | 2009-09-25 | 2017-02-14 | The Governing Council Of The University Of Toronto | Protein sequencing method and reagents |
| WO2011040971A2 (en) * | 2009-09-30 | 2011-04-07 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
| SG10201503540QA (en) | 2010-05-06 | 2015-06-29 | Ibis Biosciences Inc | Integrated sample preparation systems and stabilized enzyme mixtures |
| EP2689028B1 (en) | 2011-03-23 | 2017-08-30 | Pacific Biosciences Of California, Inc. | Isolation of polymerase-nucleic acid complexes and loading onto substrates |
| WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
| US8906660B2 (en) * | 2012-02-01 | 2014-12-09 | Pacific Biosciences Of California, Inc. | Recombinant polymerases with increased phototolerance |
| EP3590948A1 (en) | 2012-02-15 | 2020-01-08 | Pacific Biosciences of California, Inc. | Polymerase enzyme substrates with protein shield |
| US9399766B2 (en) * | 2012-10-01 | 2016-07-26 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for incorporation of protein shield nucleotide analogs |
| US9435810B2 (en) | 2013-03-15 | 2016-09-06 | Washington University | Molecules and methods for iterative polypeptide analysis and processing |
| US9678080B2 (en) | 2013-06-14 | 2017-06-13 | Pacific Biosciences Of California, Inc. | Bis-biotinylation tags |
| US10544449B2 (en) | 2013-06-14 | 2020-01-28 | Pacific Biosciences Of California, Inc. | Bis-biotinylation tags |
| US9957291B2 (en) | 2013-08-05 | 2018-05-01 | Pacific Biosciences Of California, Inc. | Protected fluorescent reagent compounds |
| WO2016055778A1 (en) | 2014-10-07 | 2016-04-14 | Oxford Nanopore Technologies Limited | Mutant pores |
| WO2016126941A1 (en) | 2015-02-04 | 2016-08-11 | Pacific Biosciences Of California, Inc. | Multimeric protected fluorescent reagents |
| US11268963B2 (en) | 2015-10-16 | 2022-03-08 | The Governing Council Of The University Of Toronto | Protein sequencing methods and reagents |
| CN108350491A (zh) | 2015-11-18 | 2018-07-31 | 加利福尼亚太平洋生物科学股份有限公司 | 将核酸加载到基材上 |
| US10676788B2 (en) | 2015-11-20 | 2020-06-09 | Pacific Biosciences Of California, Inc. | Modified nucleotide reagents |
| WO2018118997A2 (en) * | 2016-12-19 | 2018-06-28 | Quantum-Si Incorporated | Polymerizing enzymes for sequencing reactions |
| EP3655408B1 (en) | 2017-08-24 | 2021-07-14 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | Conformational restriction of cyanine fluorophores in far-red and near-ir range |
| US10836798B2 (en) | 2018-11-08 | 2020-11-17 | Government Of The United States Of America, As Represented By The Secretary Of Commerce | Amino acid-specific binder and selectively identifying an amino acid |
| EP3976771A1 (en) * | 2019-06-28 | 2022-04-06 | Quantum-si Incorporated | Polymerizing enzymes for sequencing reactions |
-
2017
- 2017-12-19 WO PCT/US2017/067427 patent/WO2018118997A2/en not_active Ceased
- 2017-12-19 CA CA3047321A patent/CA3047321A1/en active Pending
- 2017-12-19 MX MX2019007248A patent/MX2019007248A/es unknown
- 2017-12-19 KR KR1020197020661A patent/KR20190093659A/ko not_active Ceased
- 2017-12-19 CN CN201780077928.1A patent/CN110268055A/zh active Pending
- 2017-12-19 BR BR112019012181-1A patent/BR112019012181A2/pt not_active Application Discontinuation
- 2017-12-19 KR KR1020237012002A patent/KR20230052995A/ko not_active Ceased
- 2017-12-19 AU AU2017378990A patent/AU2017378990B2/en active Active
- 2017-12-19 JP JP2019533058A patent/JP2020501573A/ja active Pending
- 2017-12-19 EP EP17838018.4A patent/EP3555272A2/en not_active Withdrawn
- 2017-12-19 TW TW106144668A patent/TWI788318B/zh active
- 2017-12-19 US US15/846,967 patent/US11312944B2/en active Active
-
2022
- 2022-03-09 US US17/690,920 patent/US12545901B2/en active Active
-
2023
- 2023-01-06 JP JP2023000897A patent/JP2023026651A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3555272A2 (en) | 2019-10-23 |
| US20180362941A9 (en) | 2018-12-20 |
| US12545901B2 (en) | 2026-02-10 |
| TW201835328A (zh) | 2018-10-01 |
| US20230058852A1 (en) | 2023-02-23 |
| JP2023026651A (ja) | 2023-02-24 |
| US20180208911A1 (en) | 2018-07-26 |
| KR20230052995A (ko) | 2023-04-20 |
| WO2018118997A2 (en) | 2018-06-28 |
| CA3047321A1 (en) | 2018-06-28 |
| KR20190093659A (ko) | 2019-08-09 |
| WO2018118997A3 (en) | 2018-08-02 |
| BR112019012181A2 (pt) | 2019-11-05 |
| JP2020501573A (ja) | 2020-01-23 |
| TWI788318B (zh) | 2023-01-01 |
| AU2017378990B2 (en) | 2024-05-16 |
| US11312944B2 (en) | 2022-04-26 |
| CN110268055A (zh) | 2019-09-20 |
| AU2017378990A1 (en) | 2019-06-20 |
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