JP2019519241A5 - - Google Patents

Info

Publication number

JP2019519241A5

JP2019519241A5 JP2018568364A JP2018568364A JP2019519241A5 JP 2019519241 A5 JP2019519241 A5 JP 2019519241A5 JP 2018568364 A JP2018568364 A JP 2018568364A JP 2018568364 A JP2018568364 A JP 2018568364A JP 2019519241 A5 JP2019519241 A5 JP 2019519241A5

Authority

JP

Japan

Prior art keywords

seq

gene

library

glucose

item

Prior art date

2017-06-29

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

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• 108091033319 polynucleotide Proteins 0.000 claims description 70
• 239000002157 polynucleotide Substances 0.000 claims description 70
• 102000040430 polynucleotide Human genes 0.000 claims description 70
• 108010071189 phosphoenolpyruvate-glucose phosphotransferase Proteins 0.000 claims description 54
• 238000000034 method Methods 0.000 claims description 39
• 108090000623 proteins and genes Proteins 0.000 claims description 32
• 108700040460 Hexokinases Proteins 0.000 claims description 27
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 25
• 239000008103 glucose Substances 0.000 claims description 25
• 244000005700 microbiome Species 0.000 claims description 22
• 239000002773 nucleotide Substances 0.000 claims description 20
• 125000003729 nucleotide group Chemical group 0.000 claims description 20
• 229920001184 polypeptide Polymers 0.000 claims description 20
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 20
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 20
• 230000000694 effects Effects 0.000 claims description 14
• 241000186226 Corynebacterium glutamicum Species 0.000 claims description 9
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 8
• 108090000790 Enzymes Proteins 0.000 claims description 8
• 238000004519 manufacturing process Methods 0.000 claims description 8
• 238000012258 culturing Methods 0.000 claims description 3
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
• 108090000301 Membrane transport proteins Proteins 0.000 claims description 2
• 229910052799 carbon Inorganic materials 0.000 claims description 2
• 238000000855 fermentation Methods 0.000 claims description 2
• 230000004151 fermentation Effects 0.000 claims description 2
• 230000004048 modification Effects 0.000 claims description 2
• 238000012986 modification Methods 0.000 claims description 2
• 210000004027 cell Anatomy 0.000 claims 12
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• 230000001580 bacterial effect Effects 0.000 description 18
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
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• 150000007524 organic acids Chemical class 0.000 description 6
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• COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 3
• AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 3
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
• OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
• JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 3
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 3
• 239000004472 Lysine Substances 0.000 description 3
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 3
• AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 3
• 239000004473 Threonine Substances 0.000 description 3
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
• 229960001230 asparagine Drugs 0.000 description 3
• 235000009582 asparagine Nutrition 0.000 description 3
• 229960005261 aspartic acid Drugs 0.000 description 3
• 235000003704 aspartic acid Nutrition 0.000 description 3
• OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 3
• 235000014705 isoleucine Nutrition 0.000 description 3
• 229960000310 isoleucine Drugs 0.000 description 3
• AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 3
• 235000018977 lysine Nutrition 0.000 description 3
• 229960003646 lysine Drugs 0.000 description 3
• 235000006109 methionine Nutrition 0.000 description 3
• 229960004452 methionine Drugs 0.000 description 3
• 229930182817 methionine Natural products 0.000 description 3
• 229960005190 phenylalanine Drugs 0.000 description 3
• COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
• KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 3
• 235000008521 threonine Nutrition 0.000 description 3
• 229960002898 threonine Drugs 0.000 description 3
• 229960004799 tryptophan Drugs 0.000 description 3
• 229960004441 tyrosine Drugs 0.000 description 3
• OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 3
• 241000588724 Escherichia coli Species 0.000 description 2
• 108091028043 Nucleic acid sequence Proteins 0.000 description 2
• 238000004520 electroporation Methods 0.000 description 2
• 150000003384 small molecules Chemical class 0.000 description 2
• 238000010361 transduction Methods 0.000 description 2
• 230000026683 transduction Effects 0.000 description 2
• 230000009466 transformation Effects 0.000 description 2
• 108091000080 Phosphotransferase Proteins 0.000 description 1
• 230000015572 biosynthetic process Effects 0.000 description 1
• 102000020233 phosphotransferase Human genes 0.000 description 1
• 238000003786 synthesis reaction Methods 0.000 description 1