JP2018505688A - 標的化核酸配列包括度（ｃｏｖｅｒａｇｅ）のための方法 - Google Patents
標的化核酸配列包括度（ｃｏｖｅｒａｇｅ）のための方法 Download PDFInfo
- Publication number
- JP2018505688A JP2018505688A JP2017543973A JP2017543973A JP2018505688A JP 2018505688 A JP2018505688 A JP 2018505688A JP 2017543973 A JP2017543973 A JP 2017543973A JP 2017543973 A JP2017543973 A JP 2017543973A JP 2018505688 A JP2018505688 A JP 2018505688A
- Authority
- JP
- Japan
- Prior art keywords
- genome
- fragment
- nucleic acid
- sequence
- fragments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562119996P | 2015-02-24 | 2015-02-24 | |
| US62/119,996 | 2015-02-24 | ||
| US201562146834P | 2015-04-13 | 2015-04-13 | |
| US62/146,834 | 2015-04-13 | ||
| PCT/US2016/019382 WO2016138148A1 (en) | 2015-02-24 | 2016-02-24 | Methods for targeted nucleic acid sequence coverage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018505688A true JP2018505688A (ja) | 2018-03-01 |
| JP2018505688A5 JP2018505688A5 (enExample) | 2019-04-04 |
Family
ID=55538606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017543973A Pending JP2018505688A (ja) | 2015-02-24 | 2016-02-24 | 標的化核酸配列包括度（ｃｏｖｅｒａｇｅ）のための方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US11274343B2 (enExample) |
| EP (2) | EP3936619A1 (enExample) |
| JP (1) | JP2018505688A (enExample) |
| KR (1) | KR20170119710A (enExample) |
| CN (2) | CN107532202A (enExample) |
| AU (1) | AU2016222719B2 (enExample) |
| BR (1) | BR112017018054A2 (enExample) |
| CA (1) | CA2975958A1 (enExample) |
| IL (1) | IL254120A0 (enExample) |
| MX (1) | MX2017010857A (enExample) |
| WO (1) | WO2016138148A1 (enExample) |
Families Citing this family (85)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR102090851B1 (ko) | 2012-08-14 | 2020-03-19 | 10엑스 제노믹스, 인크. | 마이크로캡슐 조성물 및 방법 |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| DK2954065T3 (da) | 2013-02-08 | 2021-09-06 | 10X Genomics Inc | Opdeling og bearbejdning af analytter og andre arter |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| EP3161157B1 (en) | 2014-06-24 | 2024-03-27 | Bio-Rad Laboratories, Inc. | Digital pcr barcoding |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| KR102531677B1 (ko) | 2014-06-26 | 2023-05-10 | 10엑스 제노믹스, 인크. | 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법 |
| MX2016016904A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Analisis de secuencias de acidos nucleicos. |
| KR20170073667A (ko) | 2014-10-29 | 2017-06-28 | 10엑스 제노믹스, 인크. | 표적화 핵산 서열 분석을 위한 방법 및 조성물 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| KR102321863B1 (ko) | 2015-01-12 | 2021-11-08 | 10엑스 제노믹스, 인크. | 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리 |
| RU2017131622A (ru) * | 2015-02-13 | 2019-03-13 | Абхиджит Аджит ПАТЕЛ | Способы высокопараллельного и точного измерения нуклеиновых кислот |
| BR112017018054A2 (pt) * | 2015-02-24 | 2018-07-24 | 10X Genomics Inc | métodos para a cobertura de sequências de ácidos nucleicos direcionadas |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| DK3882357T3 (da) | 2015-12-04 | 2022-08-29 | 10X Genomics Inc | Fremgangsmåder og sammensætninger til analyse af nukleinsyrer |
| JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | １０エックス ジェノミクス， インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| WO2017218864A1 (en) * | 2016-06-17 | 2017-12-21 | Mayo Foundation For Medical Education And Research | Methods and materials for the effective use of combined targeted enrichment of genomic regions and low coverage whole genome sequencing |
| JP6810559B2 (ja) * | 2016-09-09 | 2021-01-06 | 株式会社日立製作所 | 環状型一本鎖核酸、およびその調製方法と使用方法 |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| CN117143960A (zh) | 2017-05-18 | 2023-12-01 | 10X基因组学有限公司 | 用于分选液滴和珠的方法和系统 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| EP3445876B1 (en) | 2017-05-26 | 2023-07-05 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
| US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| EP4241882B1 (en) | 2017-10-27 | 2025-04-30 | 10X Genomics, Inc. | Methods for sample preparation and analysis |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| CN118818037A (zh) | 2017-12-12 | 2024-10-22 | 10X基因组学有限公司 | 用于单细胞处理的系统和方法 |
| EP3728631A1 (en) | 2017-12-22 | 2020-10-28 | 10X Genomics, Inc. | Systems and methods for processing nucleic acid molecules from one or more cells |
| CN112005115A (zh) | 2018-02-12 | 2020-11-27 | 10X基因组学有限公司 | 表征来自单个细胞或细胞群体的多种分析物的方法 |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| WO2019195166A1 (en) | 2018-04-06 | 2019-10-10 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| US12188014B1 (en) | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| US12163179B2 (en) | 2018-08-03 | 2024-12-10 | 10X Gemomics, Inc. | Methods and systems to minimize barcode exchange |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| US12169198B2 (en) | 2019-01-08 | 2024-12-17 | 10X Genomics, Inc. | Systems and methods for sample analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| WO2020168013A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| US12275993B2 (en) | 2019-02-12 | 2025-04-15 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US12305239B2 (en) | 2019-02-12 | 2025-05-20 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| WO2020185791A1 (en) | 2019-03-11 | 2020-09-17 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| CN114250279B (zh) * | 2020-09-22 | 2024-04-30 | 上海韦翰斯生物医药科技有限公司 | 一种单倍型的构建方法 |
| US12480158B1 (en) | 2020-11-05 | 2025-11-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12084715B1 (en) | 2020-11-05 | 2024-09-10 | 10X Genomics, Inc. | Methods and systems for reducing artifactual antisense products |
| US12398262B1 (en) | 2021-01-22 | 2025-08-26 | 10X Genomics, Inc. | Triblock copolymer-based cell stabilization and fixation system and methods of use thereof |
| EP4298244A1 (en) | 2021-02-23 | 2024-01-03 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| US12467087B1 (en) | 2024-06-25 | 2025-11-11 | Guardant Health, Inc. | Sequencing methods with partitioning |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014210353A2 (en) * | 2013-06-27 | 2014-12-31 | 10X Technologies, Inc. | Compositions and methods for sample processing |
Family Cites Families (528)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2797149A (en) | 1953-01-08 | 1957-06-25 | Technicon International Ltd | Methods of and apparatus for analyzing liquids containing crystalloid and non-crystalloid constituents |
| US3047367A (en) | 1959-12-01 | 1962-07-31 | Technicon Instr | Automatic analysis with fluid segmentation |
| US3479141A (en) | 1967-05-17 | 1969-11-18 | Technicon Corp | Method and apparatus for analysis |
| US4124638A (en) | 1977-09-12 | 1978-11-07 | Hansen John N | Solubilizable polyacrylamide gels containing disulfide cross-linkages |
| US4253846A (en) | 1979-11-21 | 1981-03-03 | Technicon Instruments Corporation | Method and apparatus for automated analysis of fluid samples |
| GB2097692B (en) | 1981-01-10 | 1985-05-22 | Shaw Stewart P D | Combining chemical reagents |
| DE3230289A1 (de) | 1982-08-14 | 1984-02-16 | Bayer Ag, 5090 Leverkusen | Herstellung von pharmazeutischen oder kosmetischen dispersionen |
| US4582802A (en) | 1983-09-30 | 1986-04-15 | The United States Of America As Represented By The Department Of Health And Human Services | Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers |
| JPS60227826A (ja) | 1984-04-27 | 1985-11-13 | Sogo Yatsukou Kk | ｐＨに応答するグラフトカプセル |
| US4916070A (en) | 1986-04-14 | 1990-04-10 | The General Hospital Corporation | Fibrin-specific antibodies and method of screening for the antibodies |
| US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
| US4872895A (en) | 1986-12-11 | 1989-10-10 | American Telephone And Telegraph Company, At&T Bell Laboratories | Method for fabricating articles which include high silica glass bodies |
| US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
| US5137829A (en) | 1987-10-05 | 1992-08-11 | Washington University | DNA transposon TN5SEQ1 |
| US5185099A (en) | 1988-04-20 | 1993-02-09 | Institut National De Recherche Chimique Appliquee | Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics |
| US5237016A (en) | 1989-01-05 | 1993-08-17 | Siska Diagnostics, Inc. | End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids |
| US5756334A (en) | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
| DK0540693T3 (da) | 1990-07-24 | 1999-09-13 | Hoffmann La Roche | Reduktion af ikke-specifik amplifikation under in vitro-nukleinsyreamplifikation under anvendelse af modificerede nukleinsy |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US5270183A (en) | 1991-02-08 | 1993-12-14 | Beckman Research Institute Of The City Of Hope | Device and method for the automated cycling of solutions between two or more temperatures |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5770358A (en) | 1991-09-18 | 1998-06-23 | Affymax Technologies N.V. | Tagged synthetic oligomer libraries |
| US5413924A (en) | 1992-02-13 | 1995-05-09 | Kosak; Kenneth M. | Preparation of wax beads containing a reagent for release by heating |
| WO1993019205A1 (en) | 1992-03-19 | 1993-09-30 | The Regents Of The University Of California | Multiple tag labeling method for dna sequencing |
| US5587128A (en) | 1992-05-01 | 1996-12-24 | The Trustees Of The University Of Pennsylvania | Mesoscale polynucleotide amplification devices |
| WO1993022058A1 (en) | 1992-05-01 | 1993-11-11 | Trustees Of The University Of Pennsylvania | Polynucleotide amplification analysis using a microfabricated device |
| US5840865A (en) | 1992-09-14 | 1998-11-24 | Institute Of Molecular Biology And Biotechnology/Forth | Eukaryotic transposable element |
| US5569364A (en) | 1992-11-05 | 1996-10-29 | Soane Biosciences, Inc. | Separation media for electrophoresis |
| EP0682671A4 (en) | 1993-02-01 | 1998-01-14 | Seq Ltd | METHOD AND DEVICES FOR SEQUENCING DNA. |
| WO1994019101A1 (en) | 1993-02-16 | 1994-09-01 | Alliance Pharmaceutical Corp. | Method of microemulsifying fluorinated oils |
| CA2160877A1 (en) | 1993-04-19 | 1994-10-27 | Medisorb Technologies International L.P. | Long-acting treatment by slow-release delivery of antisense oligodeoxyribonucleotides from biodegradable microparticles |
| DE69429038T2 (de) | 1993-07-28 | 2002-03-21 | Pe Corporation (Ny), Norwalk | Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung |
| WO1995004069A1 (en) | 1993-07-30 | 1995-02-09 | Affymax Technologies N.V. | Biotinylation of proteins |
| US5512131A (en) | 1993-10-04 | 1996-04-30 | President And Fellows Of Harvard College | Formation of microstamped patterns on surfaces and derivative articles |
| US20030044777A1 (en) | 1993-10-28 | 2003-03-06 | Kenneth L. Beattie | Flowthrough devices for multiple discrete binding reactions |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5558071A (en) | 1994-03-07 | 1996-09-24 | Combustion Electromagnetics, Inc. | Ignition system power converter and controller |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| WO1995031726A1 (en) | 1994-05-11 | 1995-11-23 | Genera Technologies Limited | Methods of capturing species from liquids and assay procedures |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| WO1998053300A2 (en) | 1997-05-23 | 1998-11-26 | Lynx Therapeutics, Inc. | System and apparaus for sequential processing of analytes |
| US5585069A (en) | 1994-11-10 | 1996-12-17 | David Sarnoff Research Center, Inc. | Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis |
| WO1996029629A2 (en) | 1995-03-01 | 1996-09-26 | President And Fellows Of Harvard College | Microcontact printing on surfaces and derivative articles |
| US5700642A (en) | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
| HUP9900910A2 (hu) | 1995-06-07 | 1999-07-28 | Lynx Therapeutics, Inc. | Oligonukleotid jelzések osztályozáshoz és azonosításhoz |
| CZ392697A3 (cs) | 1995-06-07 | 1998-06-17 | Lynx Therapeutics, Inc. | Oligonukleotidové značky pro stanovení druhu a identifikaci |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US6866760B2 (en) | 1998-08-27 | 2005-03-15 | E Ink Corporation | Electrophoretic medium and process for the production thereof |
| US5872010A (en) | 1995-07-21 | 1999-02-16 | Northeastern University | Microscale fluid handling system |
| US6057149A (en) | 1995-09-15 | 2000-05-02 | The University Of Michigan | Microscale devices and reactions in microscale devices |
| US5851769A (en) | 1995-09-27 | 1998-12-22 | The Regents Of The University Of California | Quantitative DNA fiber mapping |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US6051377A (en) | 1995-11-30 | 2000-04-18 | Pharmaseq, Inc. | Multiplex assay for nucleic acids employing transponders |
| US6001571A (en) | 1995-11-30 | 1999-12-14 | Mandecki; Wlodek | Multiplex assay for nucleic acids employing transponders |
| US5736332A (en) | 1995-11-30 | 1998-04-07 | Mandecki; Wlodek | Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders |
| US5709153A (en) | 1995-12-29 | 1998-01-20 | Motion Controls, Inc. | High dog indexer |
| US6355198B1 (en) | 1996-03-15 | 2002-03-12 | President And Fellows Of Harvard College | Method of forming articles including waveguides via capillary micromolding and microtransfer molding |
| WO1997039359A1 (en) | 1996-04-15 | 1997-10-23 | Dade International Inc. | Apparatus and method for analysis |
| US5846727A (en) | 1996-06-06 | 1998-12-08 | Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College | Microsystem for rapid DNA sequencing |
| EP0912238B1 (en) | 1996-07-15 | 2001-10-10 | CalCiTech Ltd. | Production of powders |
| US5965443A (en) | 1996-09-09 | 1999-10-12 | Wisconsin Alumni Research Foundation | System for in vitro transposition |
| US6133436A (en) | 1996-11-06 | 2000-10-17 | Sequenom, Inc. | Beads bound to a solid support and to nucleic acids |
| US5900481A (en) | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
| EP0946749A1 (en) | 1996-11-20 | 1999-10-06 | The Regents Of The University Of Michigan | Microfabricated isothermal nucleic acid amplification devices and methods |
| US5958703A (en) | 1996-12-03 | 1999-09-28 | Glaxo Group Limited | Use of modified tethers in screening compound libraries |
| US20020172965A1 (en) | 1996-12-13 | 2002-11-21 | Arcaris, Inc. | Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom |
| US20050042625A1 (en) | 1997-01-15 | 2005-02-24 | Xzillion Gmbh & Co. | Mass label linked hybridisation probes |
| US20020034737A1 (en) | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| US6297006B1 (en) | 1997-01-16 | 2001-10-02 | Hyseq, Inc. | Methods for sequencing repetitive sequences and for determining the order of sequence subfragments |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US6391622B1 (en) | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| EP0981408B1 (en) | 1997-05-16 | 2004-04-21 | Alberta Research Council | Microfluidic system and methods of use |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US20040241759A1 (en) | 1997-06-16 | 2004-12-02 | Eileen Tozer | High throughput screening of libraries |
| ES2283936T3 (es) | 1997-07-07 | 2007-11-01 | Medical Research Council | Procedimiento de clasificacion in vitro. |
| GB9714716D0 (en) | 1997-07-11 | 1997-09-17 | Brax Genomics Ltd | Characterising nucleic acids |
| FI103809B1 (fi) | 1997-07-14 | 1999-09-30 | Finnzymes Oy | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten |
| US20050037397A1 (en) | 2001-03-28 | 2005-02-17 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US6974669B2 (en) | 2000-03-28 | 2005-12-13 | Nanosphere, Inc. | Bio-barcodes based on oligonucleotide-modified nanoparticles |
| US6368871B1 (en) | 1997-08-13 | 2002-04-09 | Cepheid | Non-planar microstructures for manipulation of fluid samples |
| AU8908198A (en) | 1997-08-15 | 1999-03-08 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| WO1999014368A2 (en) | 1997-09-15 | 1999-03-25 | Whitehead Institute For Biomedical Research | Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device |
| US20020092767A1 (en) | 1997-09-19 | 2002-07-18 | Aclara Biosciences, Inc. | Multiple array microfluidic device units |
| US7214298B2 (en) | 1997-09-23 | 2007-05-08 | California Institute Of Technology | Microfabricated cell sorter |
| WO1999018438A1 (en) | 1997-10-02 | 1999-04-15 | Aclara Biosciences, Inc. | Capillary assays involving separation of free and bound species |
| US5842787A (en) | 1997-10-09 | 1998-12-01 | Caliper Technologies Corporation | Microfluidic systems incorporating varied channel dimensions |
| US6485944B1 (en) | 1997-10-10 | 2002-11-26 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6432360B1 (en) | 1997-10-10 | 2002-08-13 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
| US6632526B1 (en) | 1997-10-14 | 2003-10-14 | Luminex Corporation | Precision fluorescently dyed particles and methods of making and using same |
| US6913935B1 (en) | 1997-12-04 | 2005-07-05 | Amersham Biosciences Uk Limited | Multiple assay method |
| AU1726199A (en) | 1997-12-31 | 1999-07-19 | Chiron Corporation | Metastatic cancer regulated gene |
| AU3196099A (en) | 1998-03-27 | 1999-10-18 | President And Fellows Of Harvard College | Systematic identification of essential genes by (in vitro) transposon mutagenesis |
| US6022716A (en) | 1998-04-10 | 2000-02-08 | Genset Sa | High throughput DNA sequencing vector |
| AU3555599A (en) | 1998-04-13 | 1999-11-01 | Luminex Corporation | Liquid labeling with fluorescent microparticles |
| US5997636A (en) | 1998-05-01 | 1999-12-07 | Instrumentation Technology Associates, Inc. | Method and apparatus for growing crystals |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6306590B1 (en) | 1998-06-08 | 2001-10-23 | Caliper Technologies Corp. | Microfluidic matrix localization apparatus and methods |
| JP2002531056A (ja) | 1998-08-07 | 2002-09-24 | セレイ， エルエルシー | 遺伝子分析におけるゲルマイクロドロップ |
| US6159736A (en) | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6296020B1 (en) | 1998-10-13 | 2001-10-02 | Biomicro Systems, Inc. | Fluid circuit components based upon passive fluid dynamics |
| US6489096B1 (en) | 1998-10-15 | 2002-12-03 | Princeton University | Quantitative analysis of hybridization patterns and intensities in oligonucleotide arrays |
| SE9803614L (sv) | 1998-10-19 | 2000-04-20 | Muhammed Mamoun | Förfarande och anordning för framställning av nanopartiklar |
| WO2000026412A1 (en) | 1998-11-02 | 2000-05-11 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US6569631B1 (en) | 1998-11-12 | 2003-05-27 | 3-Dimensional Pharmaceuticals, Inc. | Microplate thermal shift assay for ligand development using 5-(4″dimethylaminophenyl)-2-(4′-phenyl)oxazole derivative fluorescent dyes |
| NO986133D0 (no) | 1998-12-23 | 1998-12-23 | Preben Lexow | FremgangsmÕte for DNA-sekvensering |
| GB9900298D0 (en) | 1999-01-07 | 1999-02-24 | Medical Res Council | Optical sorting method |
| US6416642B1 (en) | 1999-01-21 | 2002-07-09 | Caliper Technologies Corp. | Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection |
| US6635419B1 (en) | 1999-02-16 | 2003-10-21 | Applera Corporation | Polynucleotide sequencing method |
| US20030027214A1 (en) | 1999-02-17 | 2003-02-06 | Kamb Carl Alexander | Methods for substrate-ligand interaction screening |
| ATE556149T1 (de) | 1999-02-23 | 2012-05-15 | Caliper Life Sciences Inc | Manipulation von mikropartikeln in mikrofluidischen systemen |
| US6171850B1 (en) | 1999-03-08 | 2001-01-09 | Caliper Technologies Corp. | Integrated devices and systems for performing temperature controlled reactions and analyses |
| US6303343B1 (en) | 1999-04-06 | 2001-10-16 | Caliper Technologies Corp. | Inefficient fast PCR |
| US6908737B2 (en) | 1999-04-15 | 2005-06-21 | Vitra Bioscience, Inc. | Systems and methods of conducting multiplexed experiments |
| US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| US6291243B1 (en) | 1999-04-28 | 2001-09-18 | The Board Of Trustees Of The Leland Stanford Jr. University | P element derived vector and methods for its use |
| CA2373537A1 (en) | 1999-05-12 | 2000-11-16 | Aclara Biosciences, Inc. | Multiplexed fluorescent detection in microfluidic devices |
| WO2000070095A2 (en) | 1999-05-17 | 2000-11-23 | Dade Behring Inc. | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide |
| US20020051971A1 (en) | 1999-05-21 | 2002-05-02 | John R. Stuelpnagel | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6846622B1 (en) | 1999-05-26 | 2005-01-25 | Oregon Health & Science University | Tagged epitope protein transposable element |
| US20030124509A1 (en) | 1999-06-03 | 2003-07-03 | Kenis Paul J.A. | Laminar flow patterning and articles made thereby |
| US6372813B1 (en) | 1999-06-25 | 2002-04-16 | Motorola | Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays |
| AU6068300A (en) | 1999-07-06 | 2001-01-22 | Caliper Technologies Corporation | Microfluidic systems and methods for determining modulator kinetics |
| US6977145B2 (en) | 1999-07-28 | 2005-12-20 | Serono Genetics Institute S.A. | Method for carrying out a biochemical protocol in continuous flow in a microreactor |
| US6524456B1 (en) | 1999-08-12 | 2003-02-25 | Ut-Battelle, Llc | Microfluidic devices for the controlled manipulation of small volumes |
| WO2001013086A2 (en) | 1999-08-13 | 2001-02-22 | Brandeis University | Detection of nucleic acids |
| WO2001014589A2 (en) | 1999-08-20 | 2001-03-01 | Luminex Corporation | Liquid array technology |
| WO2001016372A1 (en) | 1999-08-27 | 2001-03-08 | Matrix Technologies Corporation | Methods of immobilizing ligands on solid supports and apparatus and methods of use therefor |
| US6982146B1 (en) | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| WO2001027610A2 (en) | 1999-10-13 | 2001-04-19 | Signature Bioscience, Inc. | System and method for detecting and identifying molecular events in a test sample |
| US6958225B2 (en) | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| WO2001030965A2 (en) | 1999-10-28 | 2001-05-03 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of in vivo gene transfer using a sleeping beauty transposon system |
| HK1052203A1 (zh) | 1999-11-08 | 2003-09-05 | 荣研化学株式会社 | 检测突变和/或多态性的方法 |
| US6432290B1 (en) | 1999-11-26 | 2002-08-13 | The Governors Of The University Of Alberta | Apparatus and method for trapping bead based reagents within microfluidic analysis systems |
| US20010051348A1 (en) | 2000-01-28 | 2001-12-13 | Lee Chee Wee | Novel ligands and methods for preparing same |
| US6454924B2 (en) | 2000-02-23 | 2002-09-24 | Zyomyx, Inc. | Microfluidic devices and methods |
| IL134830A0 (en) | 2000-03-01 | 2001-05-20 | Chay 13 Medical Res Group N V | Peptides and immunostimulatory and anti-bacterial pharmaceutical compositions containing them |
| JP2003526671A (ja) | 2000-03-14 | 2003-09-09 | レストラゲン，インコーポレイテッド | 幽門洞−十二指腸運動性に対するグルカゴン様ペプチド‐１（７−３６）の作用 |
| AU2001251218B2 (en) | 2000-03-31 | 2006-06-29 | Perkinelmer Health Sciences, Inc. | Protein crystallization in microfluidic structures |
| AU2001250058A1 (en) | 2000-04-06 | 2001-10-23 | Caliper Technologies Corp. | Methods and devices for achieving long incubation times in high-throughput systems |
| US6481453B1 (en) | 2000-04-14 | 2002-11-19 | Nanostream, Inc. | Microfluidic branch metering systems and methods |
| US6800298B1 (en) | 2000-05-11 | 2004-10-05 | Clemson University | Biological lubricant composition and method of applying lubricant composition |
| US20060008799A1 (en) | 2000-05-22 | 2006-01-12 | Hong Cai | Rapid haplotyping by single molecule detection |
| CA2408574A1 (en) | 2000-05-24 | 2001-11-29 | Micronics, Inc. | Microfluidic concentration gradient loop |
| US6645432B1 (en) | 2000-05-25 | 2003-11-11 | President & Fellows Of Harvard College | Microfluidic systems including three-dimensionally arrayed channel networks |
| US20060263888A1 (en) | 2000-06-02 | 2006-11-23 | Honeywell International Inc. | Differential white blood count on a disposable card |
| US6632606B1 (en) | 2000-06-12 | 2003-10-14 | Aclara Biosciences, Inc. | Methods for single nucleotide polymorphism detection |
| AU2001272993B2 (en) | 2000-06-21 | 2005-03-10 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis |
| EP1309404A2 (en) | 2000-08-07 | 2003-05-14 | Nanostream, Inc. | Fluidic mixer in microfluidic system |
| US6773566B2 (en) | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
| US6610499B1 (en) | 2000-08-31 | 2003-08-26 | The Regents Of The University Of California | Capillary array and related methods |
| JP4949600B2 (ja) | 2000-09-14 | 2012-06-13 | カリパー・ライフ・サイエンシズ・インク． | 温度介在反応を行うためのマイクロ流体装置及び方法 |
| EP1334347A1 (en) | 2000-09-15 | 2003-08-13 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| CA2393374A1 (en) | 2000-10-10 | 2002-04-18 | Diversa Corporation | High throughput or capillary-based screening for a bioactivity or biomolecule |
| JP2002155305A (ja) | 2000-11-14 | 2002-05-31 | Akira Kawasaki | 単分散粒子の製造装置及び単分散粒子の製造方法及びその製造方法で製造された単分散粒子 |
| US6492154B2 (en) | 2001-01-31 | 2002-12-10 | Applera Corporation | Isolated human kinase proteins, nucleic acid molecules encoding human kinase proteins, and uses thereof |
| CA2332186A1 (en) | 2001-02-08 | 2002-08-08 | Her Majesty In Right Of Canada As Represented By The Minister Of Agricul Ture And Agri-Food Canada | Replicative in vivo gene targeting |
| US7670559B2 (en) | 2001-02-15 | 2010-03-02 | Caliper Life Sciences, Inc. | Microfluidic systems with enhanced detection systems |
| EP1368309B2 (en) | 2001-02-22 | 2014-10-01 | Anika Therapeutics Inc. | Thiol-modified hyaluronan |
| CA2438856C (en) | 2001-02-23 | 2007-08-07 | Japan Science And Technology Corporation | Process and apparatus for producing emulsion and microcapsules |
| US7211654B2 (en) | 2001-03-14 | 2007-05-01 | Regents Of The University Of Michigan | Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports |
| US20020159920A1 (en) | 2001-04-03 | 2002-10-31 | Weigl Bernhard H. | Multiple redundant microfluidic structures cross reference to related applications |
| US7138267B1 (en) | 2001-04-04 | 2006-11-21 | Epicentre Technologies Corporation | Methods and compositions for amplifying DNA clone copy number |
| US20030027221A1 (en) | 2001-04-06 | 2003-02-06 | Scott Melissa E. | High-throughput screening assays by encapsulation |
| US7572642B2 (en) | 2001-04-18 | 2009-08-11 | Ambrigen, Llc | Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns |
| DE60229246D1 (enExample) | 2001-05-26 | 2008-11-20 | One Cell Systems Inc | |
| US6880576B2 (en) | 2001-06-07 | 2005-04-19 | Nanostream, Inc. | Microfluidic devices for methods development |
| US7179423B2 (en) | 2001-06-20 | 2007-02-20 | Cytonome, Inc. | Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system |
| US7682353B2 (en) | 2001-06-29 | 2010-03-23 | Coloplast A/S | Catheter device |
| US6613523B2 (en) | 2001-06-29 | 2003-09-02 | Agilent Technologies, Inc. | Method of DNA sequencing using cleavable tags |
| US7077152B2 (en) | 2001-07-07 | 2006-07-18 | Nanostream, Inc. | Microfluidic metering systems and methods |
| US6767731B2 (en) | 2001-08-27 | 2004-07-27 | Intel Corporation | Electron induced fluorescent method for nucleic acid sequencing |
| US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US6783647B2 (en) | 2001-10-19 | 2004-08-31 | Ut-Battelle, Llc | Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate |
| CA2464350A1 (en) | 2001-10-19 | 2003-05-30 | West Virginia University Research Corporation | Microfluidic system for proteome analysis |
| US20030149307A1 (en) | 2001-10-24 | 2003-08-07 | Baxter International Inc. | Process for the preparation of polyethylene glycol bis amine |
| WO2003038558A2 (en) | 2001-10-30 | 2003-05-08 | Nanomics Biosystems Pty, Ltd. | Device and methods for directed synthesis of chemical libraries |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| AU2002351193A1 (en) | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| US7335153B2 (en) | 2001-12-28 | 2008-02-26 | Bio Array Solutions Ltd. | Arrays of microparticles and methods of preparation thereof |
| AU2003210438A1 (en) | 2002-01-04 | 2003-07-24 | Board Of Regents, The University Of Texas System | Droplet-based microfluidic oligonucleotide synthesis engine |
| AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| KR100459870B1 (ko) | 2002-02-22 | 2004-12-04 | 한국과학기술원 | 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법 |
| US20050202429A1 (en) | 2002-03-20 | 2005-09-15 | Innovativebio.Biz | Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartment for parallels reactions |
| US7901939B2 (en) | 2002-05-09 | 2011-03-08 | University Of Chicago | Method for performing crystallization and reactions in pressure-driven fluid plugs |
| DE60333995D1 (de) | 2002-05-09 | 2010-10-14 | Univ Chicago | Einrichtugn und verfahren für druckgesteuerten plug-transport und reaktion |
| US7527966B2 (en) | 2002-06-26 | 2009-05-05 | Transgenrx, Inc. | Gene regulation in transgenic animals using a transposon-based vector |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| AU2003252136A1 (en) | 2002-07-24 | 2004-02-09 | Ptc Therapeutics, Inc. | METHODS FOR IDENTIFYING SMALL MOLEDULES THAT MODULATE PREMATURE TRANSLATION TERMINATION AND NONSENSE MEDIATED mRNA DECAY |
| IL151660A0 (en) | 2002-09-09 | 2003-04-10 | Univ Ben Gurion | Method for isolating and culturing unculturable microorganisms |
| US20040081962A1 (en) | 2002-10-23 | 2004-04-29 | Caifu Chen | Methods for synthesizing complementary DNA |
| US20040248299A1 (en) | 2002-12-27 | 2004-12-09 | Sumedha Jayasena | RNA interference |
| DE602004021902D1 (de) | 2003-01-17 | 2009-08-20 | Univ Boston | Haplotypanalyse |
| AU2004209001B2 (en) | 2003-01-29 | 2007-10-11 | 454 Life Sciences Corporation | Bead emulsion nucleic acid amplification |
| EP1594973B1 (en) | 2003-02-10 | 2011-12-07 | Max-Delbrück-Centrum für Molekulare Medizin (MDC) | Transposon-based targeting system |
| US20100022414A1 (en) | 2008-07-18 | 2010-01-28 | Raindance Technologies, Inc. | Droplet Libraries |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| US7316903B2 (en) | 2003-03-28 | 2008-01-08 | United States Of America As Represented By The Department Of Health And Human Services | Detection of nucleic acid sequence variations using phase Mu transposase |
| GB0307403D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Selection by compartmentalised screening |
| GB0307428D0 (en) | 2003-03-31 | 2003-05-07 | Medical Res Council | Compartmentalised combinatorial chemistry |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| PL1613346T3 (pl) | 2003-04-04 | 2013-03-29 | Zoetis Services Llc | Poddane mikrofluidyzacji emulsje typu olej w wodzie i kompozycje szczepionek |
| US20100035254A1 (en) | 2003-04-08 | 2010-02-11 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| EP2127736A1 (en) | 2003-04-10 | 2009-12-02 | The President and Fellows of Harvard College | Formation and control of fluidic species |
| EP1629286A1 (en) | 2003-05-16 | 2006-03-01 | Global Technologies (NZ) Ltd. | Method and apparatus for mixing sample and reagent in a suspension fluid |
| WO2004103565A2 (de) | 2003-05-19 | 2004-12-02 | Hans-Knöll-Institut für Naturstoff-Forschung e.V. | Vorrichtung und verfahren zur strukturierung von flüssigkeiten und zum zudosieren von reaktionsflüssigkeiten zu in separationsmedium eingebetteten flüssigkeitskompartimenten |
| WO2004105734A1 (en) | 2003-05-28 | 2004-12-09 | Valorisation Recherche, Societe En Commandite | Method of preparing microcapsules |
| JP2007503597A (ja) | 2003-06-13 | 2007-02-22 | ザ ジェネラル ホスピタル コーポレーション | 血液から赤血球および血小板をサイズに基づいて除去するための微少流体システム |
| GB2403475B (en) | 2003-07-01 | 2008-02-06 | Oxitec Ltd | Stable integrands |
| GB0315438D0 (en) | 2003-07-02 | 2003-08-06 | Univ Manchester | Analysis of mixed cell populations |
| JP5183063B2 (ja) | 2003-07-05 | 2013-04-17 | ザ ジョンズ ホプキンス ユニバーシティ | 遺伝的変異の検出および列挙のための方法ならびに組成物 |
| BRPI0414004A (pt) | 2003-08-27 | 2006-10-24 | Harvard College | controle eletrÈnico de espécies fluìdicas |
| JP4988345B2 (ja) | 2003-09-04 | 2012-08-01 | ザ・ユナイテッド・ステイツ・オブ・アメリカ・アズ・リプレゼンティッド・バイ・ザ・デパートメント・オブ・ヴェテランズ・アフェアーズ | 眼用ハイドロゲルナノコンポジット |
| JP4353945B2 (ja) | 2003-09-22 | 2009-10-28 | 独立行政法人理化学研究所 | 効率的なｄｎａ逆位反復構造の調製方法 |
| EP2381255A1 (en) | 2003-09-25 | 2011-10-26 | Toyama Prefecture | Microwell array chip and its manufacturing method |
| EP1691792A4 (en) | 2003-11-24 | 2008-05-28 | Yeda Res & Dev | COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES |
| US20050136417A1 (en) | 2003-12-19 | 2005-06-23 | Affymetrix, Inc. | Amplification of nucleic acids |
| US8071364B2 (en) | 2003-12-24 | 2011-12-06 | Transgenrx, Inc. | Gene therapy using transposon-based vectors |
| US20050181379A1 (en) | 2004-02-18 | 2005-08-18 | Intel Corporation | Method and device for isolating and positioning single nucleic acid molecules |
| CN1950519A (zh) | 2004-02-27 | 2007-04-18 | 哈佛大学的校长及成员们 | 聚合酶群落荧光原位测序珠子 |
| KR100552706B1 (ko) | 2004-03-12 | 2006-02-20 | 삼성전자주식회사 | 핵산 증폭 방법 및 장치 |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| EP1735668A2 (en) | 2004-04-13 | 2006-12-27 | President And Fellows Of Harvard College | Methods and apparatus for manipulation and/or detection of biological samples and other objects |
| US20080268507A1 (en) | 2004-05-25 | 2008-10-30 | Airbus Deutschland Gmbh | Recombinant Dna Nicking Endonuclease and Uses Thereof |
| US7799553B2 (en) | 2004-06-01 | 2010-09-21 | The Regents Of The University Of California | Microfabricated integrated DNA analysis system |
| US7700281B2 (en) | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| US7968085B2 (en) | 2004-07-05 | 2011-06-28 | Ascendis Pharma A/S | Hydrogel formulations |
| CN1648671B (zh) | 2005-02-06 | 2012-09-26 | 成都夸常医学工业有限公司 | 多反应器分析芯片检测方法和分析芯片及检测装置 |
| US7608434B2 (en) | 2004-08-04 | 2009-10-27 | Wisconsin Alumni Research Foundation | Mutated Tn5 transposase proteins and the use thereof |
| US20080268431A1 (en) | 2004-09-14 | 2008-10-30 | Jin-Ho Choy | Information Code System Using Dna Sequences |
| US7892731B2 (en) | 2004-10-01 | 2011-02-22 | Radix Biosolutions, Ltd. | System and method for inhibiting the decryption of a nucleic acid probe sequence used for the detection of a specific nucleic acid |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| US9492400B2 (en) | 2004-11-04 | 2016-11-15 | Massachusetts Institute Of Technology | Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals |
| WO2006051552A2 (en) | 2004-11-15 | 2006-05-18 | Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science | Directed evolution and selection using in vitro compartmentalization |
| US7329493B2 (en) | 2004-12-22 | 2008-02-12 | Asiagen Corporation | One-tube nested PCR for detecting Mycobacterium tuberculosis |
| US20080213593A1 (en) | 2005-01-21 | 2008-09-04 | President And Fellows Of Harvard College | Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles |
| EP1841879A4 (en) | 2005-01-25 | 2009-05-27 | Population Genetics Technologi | ISOTHERMAL DNA AMPLIFICATION |
| US7407757B2 (en) | 2005-02-10 | 2008-08-05 | Population Genetics Technologies | Genetic analysis by sequence-specific sorting |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| KR101544351B1 (ko) | 2005-02-18 | 2015-08-13 | 캐논 유.에스. 라이프 사이언시즈, 인크. | 유기체의 게놈 ｄｎａ를 확인하기 위한 장치 및 방법 |
| EP1867702B1 (en) | 2005-02-21 | 2011-09-28 | Kagoshima University | Method for purifying biodiesel fuel |
| US20070054119A1 (en) | 2005-03-04 | 2007-03-08 | Piotr Garstecki | Systems and methods of forming particles |
| EP2248578B1 (en) | 2005-03-04 | 2012-06-06 | President and Fellows of Harvard College | Method for forming multiple emulsions |
| US9040237B2 (en) | 2005-03-04 | 2015-05-26 | Intel Corporation | Sensor arrays and nucleic acid sequencing applications |
| JP2006289250A (ja) | 2005-04-08 | 2006-10-26 | Kao Corp | マイクロミキサー及びそれを用いた流体混合方法 |
| US20090264299A1 (en) | 2006-02-24 | 2009-10-22 | Complete Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| EP3257949A1 (en) | 2005-06-15 | 2017-12-20 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| JP2006349060A (ja) | 2005-06-16 | 2006-12-28 | Ntn Corp | ボールねじ |
| WO2007002490A2 (en) | 2005-06-22 | 2007-01-04 | The Research Foundation Of State University Of New York | Massively parallel 2-dimensional capillary electrophoresis |
| WO2007002567A2 (en) | 2005-06-23 | 2007-01-04 | Nanosphere, Inc. | Selective isolation and concentration of nucleic acids from complex samples |
| EP2392657B1 (en) | 2005-07-05 | 2013-09-25 | The Chemo-Sero-Therapeutic Research Institute | Modified transposon vector and its use |
| JP5051490B2 (ja) | 2005-07-08 | 2012-10-17 | 独立行政法人産業技術総合研究所 | マクロ生体材料を内包する無機マイクロカプセルおよびその製造方法 |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| FR2888912B1 (fr) | 2005-07-25 | 2007-08-24 | Commissariat Energie Atomique | Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif |
| US20070031857A1 (en) | 2005-08-02 | 2007-02-08 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction |
| WO2007024840A2 (en) | 2005-08-22 | 2007-03-01 | Critical Therapeutics, Inc. | Method of quantitating nucleic acids by flow cytometry microparticle-based array |
| JP2007074967A (ja) | 2005-09-13 | 2007-03-29 | Canon Inc | 識別子プローブ及びそれを用いた核酸増幅方法 |
| CN101523156A (zh) | 2005-09-16 | 2009-09-02 | 加利福尼亚大学董事会 | 用于分析物检测的比色生物条形码扩增测定 |
| US7960104B2 (en) | 2005-10-07 | 2011-06-14 | Callida Genomics, Inc. | Self-assembled single molecule arrays and uses thereof |
| WO2007120265A2 (en) | 2005-11-14 | 2007-10-25 | Applera Corporation | Coded molecules for detecting target analytes |
| US20070134277A1 (en) | 2005-12-09 | 2007-06-14 | Children's Medical Center Corporation | Pharmaceutical formulation for sulfur-containing drugs in liquid dosage forms |
| AU2006325338B2 (en) | 2005-12-15 | 2012-02-09 | Akzo Nobel Coatings International B.V. | Multilayer coating system |
| US20070141584A1 (en) | 2005-12-20 | 2007-06-21 | Roberts Douglas N | Methods for assessment of native chromatin on microarrays |
| US7932037B2 (en) | 2007-12-05 | 2011-04-26 | Perkinelmer Health Sciences, Inc. | DNA assays using amplicon probes on encoded particles |
| EP1984738A2 (en) | 2006-01-11 | 2008-10-29 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| WO2007087310A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
| WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
| AU2007210152A1 (en) | 2006-01-27 | 2007-08-09 | President And Fellows Of Harvard College | Fluidic droplet coalescence |
| SI3002338T1 (sl) | 2006-02-02 | 2019-11-29 | Univ Leland Stanford Junior | Neinvaziven genetski pregled zarodka z digitalno analizo |
| WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| GB0603251D0 (en) | 2006-02-17 | 2006-03-29 | Isis Innovation | DNA conformation |
| EP2495337A1 (en) | 2006-02-24 | 2012-09-05 | Callida Genomics, Inc. | High throughput genome sequencing on DNA arrays |
| WO2007111937A1 (en) | 2006-03-23 | 2007-10-04 | Applera Corporation | Directed enrichment of genomic dna for high-throughput sequencing |
| JP4921829B2 (ja) | 2006-03-30 | 2012-04-25 | 株式会社東芝 | 微粒子の製造装置、乳化剤保持部、微粒子の製造方法および分子膜の製造方法 |
| WO2007114794A1 (en) | 2006-03-31 | 2007-10-11 | Nam Trung Nguyen | Active control for droplet-based microfluidics |
| JP2009538123A (ja) | 2006-04-19 | 2009-11-05 | アプライド バイオシステムズ， エルエルシー | ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー |
| US7811603B2 (en) | 2006-05-09 | 2010-10-12 | The Regents Of The University Of California | Microfluidic device for forming monodisperse lipoplexes |
| EP4190448A3 (en) | 2006-05-11 | 2023-09-20 | Bio-Rad Laboratories, Inc. | Microfluidic devices |
| ES2620398T3 (es) | 2006-05-22 | 2017-06-28 | Nanostring Technologies, Inc. | Sistemas y métodos para analizar nanoindicadores |
| US20080124726A1 (en) | 2006-05-26 | 2008-05-29 | Althea Technologies, Inc. | Biochemical analysis of partitioned cells |
| FR2901717A1 (fr) | 2006-05-30 | 2007-12-07 | Centre Nat Rech Scient | Procede de traitement de gouttes dans un circuit microfluidique. |
| EP3424598B1 (en) | 2006-06-14 | 2022-06-08 | Verinata Health, Inc. | Rare cell analysis using sample splitting and dna tags |
| AU2007261445B2 (en) | 2006-06-19 | 2013-04-18 | The Johns Hopkins University | Single-molecule PCR on microparticles in water-in-oil emulsions |
| US20080076909A1 (en) | 2006-06-30 | 2008-03-27 | Applera Corporation | Emulsion pcr and amplicon capture |
| EP1878501A1 (en) | 2006-07-14 | 2008-01-16 | Roche Diagnostics GmbH | Instrument for heating and cooling |
| US9012390B2 (en) | 2006-08-07 | 2015-04-21 | Raindance Technologies, Inc. | Fluorocarbon emulsion stabilizing surfactants |
| WO2008037082A1 (en) | 2006-09-25 | 2008-04-03 | Archer-Daniels-Midland Company | Superabsorbent surface-treated carboxyalkylated polysaccharides and process for producing same |
| US7935518B2 (en) | 2006-09-27 | 2011-05-03 | Alessandra Luchini | Smart hydrogel particles for biomarker harvesting |
| US8841116B2 (en) | 2006-10-25 | 2014-09-23 | The Regents Of The University Of California | Inline-injection microdevice and microfabricated integrated DNA analysis system using same |
| WO2008070352A2 (en) | 2006-10-27 | 2008-06-12 | Complete Genomics, Inc. | Efficient arrays of amplified polynucleotides |
| EP2082063B1 (en) | 2006-11-15 | 2014-05-07 | Biospherex LLC | Multitag sequencing and ecogenomics analysis |
| US20080242560A1 (en) | 2006-11-21 | 2008-10-02 | Gunderson Kevin L | Methods for generating amplified nucleic acid arrays |
| JP5320546B2 (ja) | 2006-12-13 | 2013-10-23 | 国立大学法人名古屋大学 | Ｔｏｌ１因子のトランスポザーゼ及びそれを用いたＤＮＡ導入システム |
| US7844658B2 (en) | 2007-01-22 | 2010-11-30 | Comcast Cable Holdings, Llc | System and method for providing an application to a device |
| US20080176768A1 (en) | 2007-01-23 | 2008-07-24 | Honeywell Honeywell International | Hydrogel microarray with embedded metal nanoparticles |
| EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| US8003312B2 (en) | 2007-02-16 | 2011-08-23 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplex cellular assays using detectable cell barcodes |
| FI20075124A0 (fi) | 2007-02-21 | 2007-02-21 | Valtion Teknillinen | Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi |
| US9029085B2 (en) | 2007-03-07 | 2015-05-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| US20080228268A1 (en) | 2007-03-15 | 2008-09-18 | Uluru, Inc. | Method of Formation of Viscous, Shape Conforming Gels and Their Uses as Medical Prosthesis |
| EP2136786B8 (en) | 2007-03-28 | 2012-11-14 | President and Fellows of Harvard College | Apparatus for forming droplets |
| US9222936B2 (en) | 2007-04-18 | 2015-12-29 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization |
| WO2008134153A1 (en) | 2007-04-23 | 2008-11-06 | Advanced Liquid Logic, Inc. | Bead-based multiplexed analytical methods and instrumentation |
| CN101293191B (zh) | 2007-04-25 | 2011-11-09 | 中国科学院过程工程研究所 | 一种琼脂糖凝胶微球的制备方法 |
| WO2008135512A2 (en) | 2007-05-02 | 2008-11-13 | Jerzy Paszkowski | Dna amplification method |
| JP2010528608A (ja) | 2007-06-01 | 2010-08-26 | ４５４ ライフ サイエンシーズ コーポレイション | 複合的な混合物から個々の試料を特定するためのシステムおよび方法 |
| US20100255556A1 (en) | 2007-06-29 | 2010-10-07 | President And Fellows Of Harvard College | Methods and apparatus for manipulation of fluidic species |
| WO2009011808A1 (en) | 2007-07-13 | 2009-01-22 | President And Fellows Of Harvard College | Droplet-based selection |
| US8454906B2 (en) | 2007-07-24 | 2013-06-04 | The Regents Of The University Of California | Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions |
| US20130084243A1 (en) | 2010-01-27 | 2013-04-04 | Liliane Goetsch | Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders |
| US8563527B2 (en) | 2007-08-20 | 2013-10-22 | Pharmain Corporation | Oligonucleotide core carrier compositions for delivery of nucleic acid-containing therapeutic agents, methods of making and using the same |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| WO2009048532A2 (en) | 2007-10-05 | 2009-04-16 | President And Fellows Of Harvard College | Formation of particles for ultrasound application, drug release, and other uses, and microfluidic methods of preparation |
| US20090099040A1 (en) | 2007-10-15 | 2009-04-16 | Sigma Aldrich Company | Degenerate oligonucleotides and their uses |
| US20100086914A1 (en) | 2008-10-03 | 2010-04-08 | Roche Molecular Systems, Inc. | High resolution, high throughput hla genotyping by clonal sequencing |
| WO2009061372A1 (en) | 2007-11-02 | 2009-05-14 | President And Fellows Of Harvard College | Systems and methods for creating multi-phase entities, including particles and/or fluids |
| US8592150B2 (en) | 2007-12-05 | 2013-11-26 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
| CN101918590B (zh) | 2007-12-10 | 2013-03-27 | 高晓莲 | 核酸测序 |
| US7771944B2 (en) | 2007-12-14 | 2010-08-10 | The Board Of Trustees Of The University Of Illinois | Methods for determining genetic haplotypes and DNA mapping |
| US9797010B2 (en) | 2007-12-21 | 2017-10-24 | President And Fellows Of Harvard College | Systems and methods for nucleic acid sequencing |
| EP3699291B1 (en) | 2008-01-17 | 2024-08-07 | Sequenom, Inc. | Single molecule nucleic acid sequence analysis processes and compositions |
| EP2252625B1 (en) | 2008-02-07 | 2017-12-20 | Pacific Biosciences of California, Inc. | Cis reactive oxygen quenchers integrated into linkers |
| JP5468271B2 (ja) | 2008-02-08 | 2014-04-09 | 花王株式会社 | 微粒子分散液の製造方法 |
| US20090203531A1 (en) | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
| US9068181B2 (en) | 2008-05-23 | 2015-06-30 | The General Hospital Corporation | Microfluidic droplet encapsulation |
| GB0810051D0 (en) | 2008-06-02 | 2008-07-09 | Oxford Biodynamics Ltd | Method of diagnosis |
| KR20110042050A (ko) | 2008-06-05 | 2011-04-22 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 폴리머좀, 콜로이드좀, 리포좀 및 유체 액적과 관련된 다른 종 |
| EP2291533B2 (en) | 2008-07-02 | 2020-09-30 | Illumina Cambridge Limited | Using populations of beads for the fabrication of arrays on surfaces |
| EP2303245B1 (en) | 2008-07-11 | 2016-12-28 | ETH Zurich | Degradable microcapsules |
| US20100062494A1 (en) | 2008-08-08 | 2010-03-11 | President And Fellows Of Harvard College | Enzymatic oligonucleotide pre-adenylation |
| US8383345B2 (en) | 2008-09-12 | 2013-02-26 | University Of Washington | Sequence tag directed subassembly of short sequencing reads into long sequencing reads |
| WO2010033200A2 (en) | 2008-09-19 | 2010-03-25 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
| US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| WO2013016459A1 (en) | 2011-07-25 | 2013-01-31 | Bio-Rad Laboratories, Inc. | Breakage of an emulsion containing nucleic acid |
| US20120252015A1 (en) | 2011-02-18 | 2012-10-04 | Bio-Rad Laboratories | Methods and compositions for detecting genetic material |
| US9194861B2 (en) | 2009-09-02 | 2015-11-24 | Bio-Rad Laboratories, Inc. | Method of mixing fluids by coalescence of multiple emulsions |
| AU2009296734B2 (en) | 2008-09-25 | 2016-02-18 | Cephalon Llc | Liquid formulations of bendamustine |
| US8361299B2 (en) | 2008-10-08 | 2013-01-29 | Sage Science, Inc. | Multichannel preparative electrophoresis system |
| DK2787565T3 (en) | 2008-10-24 | 2015-10-12 | Epict Technologies Corp | Transposon-end COMPOSITIONS AND METHODS TO MODIFY nucleic acids |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US20100113296A1 (en) | 2008-11-05 | 2010-05-06 | Joel Myerson | Methods And Kits For Nucleic Acid Analysis |
| US8748103B2 (en) | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| EP3150724A1 (en) | 2008-12-19 | 2017-04-05 | President and Fellows of Harvard College | Particle-assisted nucleic acid sequencing |
| KR101065807B1 (ko) | 2009-01-23 | 2011-09-19 | 충남대학교산학협력단 | 액적 기반의 미세유체 칩을 이용한 마이크로 캡슐 제조방법 |
| US9347092B2 (en) | 2009-02-25 | 2016-05-24 | Roche Molecular System, Inc. | Solid support for high-throughput nucleic acid analysis |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| WO2010104604A1 (en) | 2009-03-13 | 2010-09-16 | President And Fellows Of Harvard College | Method for the controlled creation of emulsions, including multiple emulsions |
| US9056299B2 (en) | 2009-03-13 | 2015-06-16 | President And Fellows Of Harvard College | Scale-up of flow-focusing microfluidic devices |
| US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| ES2706227T3 (es) | 2009-03-30 | 2019-03-27 | Illumina Inc | Análisis de expresión génica en células individuales |
| EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
| FR2945819B1 (fr) | 2009-05-19 | 2011-06-17 | Commissariat Energie Atomique | Dispositif et procede d'isolement de cibles biologiques ou chimiques |
| US8574835B2 (en) | 2009-05-29 | 2013-11-05 | Life Technologies Corporation | Scaffolded nucleic acid polymer particles and methods of making and using |
| US9524369B2 (en) | 2009-06-15 | 2016-12-20 | Complete Genomics, Inc. | Processing and analysis of complex nucleic acid sequence data |
| CN102459592B (zh) | 2009-06-15 | 2017-04-05 | 考利达基因组股份有限公司 | 用于长片段阅读测序的方法和组合物 |
| EP4019977B1 (en) | 2009-06-26 | 2024-11-20 | President and Fellows of Harvard College | Fluid injection |
| US20110028412A1 (en) | 2009-08-03 | 2011-02-03 | Cappellos, Inc. | Herbal enhanced analgesic formulations |
| US20110033548A1 (en) | 2009-08-05 | 2011-02-10 | E.I. Du Pont De Nemours And Company | Degradable crosslinked aminated dextran microspheres and methods of use |
| ES2562159T3 (es) | 2009-08-20 | 2016-03-02 | Population Genetics Technologies Ltd. | Composiciones y métodos para el reordenamiento de ácido nucleico molecular |
| US20120211084A1 (en) | 2009-09-02 | 2012-08-23 | President And Fellows Of Harvard College | Multiple emulsions created using jetting and other techniques |
| US9625454B2 (en) | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| GB0918564D0 (en) | 2009-10-22 | 2009-12-09 | Plasticell Ltd | Nested cell encapsulation |
| EP2493619B1 (en) | 2009-10-27 | 2018-12-19 | President and Fellows of Harvard College | Droplet creation techniques |
| US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
| EP2496700B1 (en) | 2009-11-04 | 2017-03-01 | The University Of British Columbia | Nucleic acid-containing lipid particles and related methods |
| CA2767028A1 (en) | 2009-11-25 | 2011-06-03 | Quantalife, Inc. | Methods and compositions for detecting genetic material |
| JP2013511991A (ja) | 2009-11-25 | 2013-04-11 | クアンタライフ， インコーポレイテッド | 遺伝子材料を検出する方法および組成物 |
| US9023769B2 (en) | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CN102656279A (zh) | 2009-12-17 | 2012-09-05 | 凯津公司 | 基于全基因组测序的限制性酶 |
| EP2517025B1 (en) | 2009-12-23 | 2019-11-27 | Bio-Rad Laboratories, Inc. | Methods for reducing the exchange of molecules between droplets |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| EP2550528B1 (en) | 2010-03-25 | 2019-09-11 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
| US20120000777A1 (en) | 2010-06-04 | 2012-01-05 | The Regents Of The University Of California | Devices and methods for forming double emulsion droplet compositions and polymer particles |
| US8703493B2 (en) | 2010-06-15 | 2014-04-22 | Src, Inc. | Location analysis using fire retardant-protected nucleic acid-labeled tags |
| US20120003657A1 (en) | 2010-07-02 | 2012-01-05 | Samuel Myllykangas | Targeted sequencing library preparation by genomic dna circularization |
| US20120238738A1 (en) | 2010-07-19 | 2012-09-20 | New England Biolabs, Inc. | Oligonucleotide Adapters: Compositions and Methods of Use |
| CN103202812B (zh) | 2010-08-09 | 2015-10-28 | 南京大学 | 一种制备用于体内递送药理活性物质的蛋白纳米粒的方法 |
| US20130203675A1 (en) | 2010-09-16 | 2013-08-08 | Joseph M. DeSimone | Asymmetric biofunctional silyl monomers and particles thereof as prodrugs and delivery vehicles for pharmaceutical, chemical and biological agents |
| ES2523140T3 (es) | 2010-09-21 | 2014-11-21 | Population Genetics Technologies Ltd. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| WO2012047889A2 (en) | 2010-10-04 | 2012-04-12 | Genapsys Inc. | Systems and methods for automated reusable parallel biological reactions |
| US9999886B2 (en) | 2010-10-07 | 2018-06-19 | The Regents Of The University Of California | Methods and systems for on demand droplet generation and impedance based detection |
| WO2012048341A1 (en) | 2010-10-08 | 2012-04-12 | President And Fellows Of Harvard College | High-throughput single cell barcoding |
| ES2730951T3 (es) | 2010-10-08 | 2019-11-13 | Harvard College | Secuenciación inmune de alto rendimiento |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| CA2821299C (en) | 2010-11-05 | 2019-02-12 | Frank J. Steemers | Linking sequence reads using paired code tags |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US20140057799A1 (en) | 2010-12-16 | 2014-02-27 | Gigagen | System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells |
| US8765455B2 (en) | 2011-01-27 | 2014-07-01 | Lawrence Livermore National Security, Llc | Chip-based droplet sorting |
| ES2762866T3 (es) | 2011-01-28 | 2020-05-26 | Illumina Inc | Reemplazo de nucleótidos por bibliotecas doblemente etiquetadas y direccionales |
| JP6017458B2 (ja) | 2011-02-02 | 2016-11-02 | ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション | 大量並列連続性マッピング |
| EP2675819B1 (en) | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| GB2489714B (en) | 2011-04-05 | 2013-11-06 | Tracesa Ltd | Fluid Identification Method |
| EP2702175B1 (en) | 2011-04-25 | 2018-08-08 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| EP2702170B1 (en) * | 2011-04-28 | 2015-12-16 | Life Technologies Corporation | Methods and compositions for multiplex pcr |
| EP3421592B1 (en) | 2011-04-28 | 2023-09-13 | The Board of Trustees of the Leland Stanford Junior University | Identification of polynucleotides associated with a sample |
| WO2012157684A1 (ja) | 2011-05-16 | 2012-11-22 | 地方独立行政法人 大阪府立病院機構 | 血中ｄｎａの定量的検出による悪性新生物の病勢の進行を評価する方法 |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| EP2714254B1 (en) | 2011-05-23 | 2017-09-06 | President and Fellows of Harvard College | Control of emulsions, including multiple emulsions |
| US9617598B2 (en) | 2011-05-27 | 2017-04-11 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US9150916B2 (en) | 2011-06-24 | 2015-10-06 | Beat Christen | Compositions and methods for identifying the essential genome of an organism |
| US8927218B2 (en) | 2011-06-27 | 2015-01-06 | Flir Systems, Inc. | Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples |
| WO2013006824A2 (en) | 2011-07-07 | 2013-01-10 | Life Technologies Corporation | Polymer particles, nucleic acid polymer particles and methods of making and using the same |
| US20130017978A1 (en) | 2011-07-11 | 2013-01-17 | Finnzymes Oy | Methods and transposon nucleic acids for generating a dna library |
| US9605304B2 (en) | 2011-07-20 | 2017-03-28 | The Hong Kong Polytechnic University | Ultra-stable oligonucleotide-gold and-silver nanoparticle conjugates and method of their preparation |
| WO2013019751A1 (en) | 2011-07-29 | 2013-02-07 | Bio-Rad Laboratories, Inc., | Library characterization by digital assay |
| CA2848304A1 (en) | 2011-09-09 | 2013-03-14 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for sequencing a polynucleotide |
| US20130109596A1 (en) | 2011-09-26 | 2013-05-02 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US9514272B2 (en) | 2011-10-12 | 2016-12-06 | Complete Genomics, Inc. | Identification of DNA fragments and structural variations |
| US9469874B2 (en) * | 2011-10-18 | 2016-10-18 | The Regents Of The University Of California | Long-range barcode labeling-sequencing |
| US20130109576A1 (en) | 2011-10-28 | 2013-05-02 | Anthony P. Shuber | Methods for detecting mutations |
| EP2774277A4 (en) | 2011-11-04 | 2015-07-22 | Intel Corp | SIGNALING FOR THE CONFIGURATION OF COORDINATED DOWNLINK MULTIPORT COMMUNICATIONS |
| CN103946548B (zh) | 2011-11-16 | 2016-10-05 | 国际商业机器公司 | 具有可变形阀的微流体器件 |
| US10689643B2 (en) | 2011-11-22 | 2020-06-23 | Active Motif, Inc. | Targeted transposition for use in epigenetic studies |
| US9938524B2 (en) | 2011-11-22 | 2018-04-10 | Active Motif, Inc. | Multiplex isolation of protein-associated nucleic acids |
| EP2794928B1 (en) | 2011-12-22 | 2019-02-20 | President and Fellows of Harvard College | Compositions and methods for analyte detection |
| US20150125865A1 (en) | 2011-12-23 | 2015-05-07 | Gigagen, Inc. | Methods And Apparatuses For Droplet Mixing |
| US9243085B2 (en) | 2012-02-09 | 2016-01-26 | Life Technologies Corporation | Hydrophilic polymeric particles and methods for making and using same |
| US9637781B2 (en) | 2012-02-14 | 2017-05-02 | The Johns Hopkins University | MiRNA analysis methods |
| WO2013123382A1 (en) | 2012-02-15 | 2013-08-22 | Wisconsin Alumni Research Foundation | Dithioamine reducing agents |
| US10202628B2 (en) | 2012-02-17 | 2019-02-12 | President And Fellows Of Harvard College | Assembly of nucleic acid sequences in emulsions |
| US9176031B2 (en) | 2012-02-24 | 2015-11-03 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
| EP2823064B1 (en) | 2012-03-05 | 2019-02-06 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| NO2694769T3 (enExample) | 2012-03-06 | 2018-03-03 | ||
| EP2647426A1 (en) | 2012-04-03 | 2013-10-09 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Replication of distributed nucleic acid molecules with preservation of their relative distribution through hybridization-based binding |
| US20130296173A1 (en) | 2012-04-23 | 2013-11-07 | Complete Genomics, Inc. | Pre-anchor wash |
| WO2013177220A1 (en) | 2012-05-21 | 2013-11-28 | The Scripps Research Institute | Methods of sample preparation |
| WO2013188872A1 (en) | 2012-06-15 | 2013-12-19 | The Board Of Regents Of The University Of Texas System | High throughput sequencing of multiple transcripts of a single cell |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20150005199A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20150005200A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR102090851B1 (ko) | 2012-08-14 | 2020-03-19 | 10엑스 제노믹스, 인크. | 마이크로캡슐 조성물 및 방법 |
| US20140378322A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| EP2898096B1 (en) | 2012-09-21 | 2024-02-14 | The Broad Institute, Inc. | Methods for labeling of rnas |
| US9644199B2 (en) | 2012-10-01 | 2017-05-09 | Agilent Technologies, Inc. | Immobilized transposase complexes for DNA fragmentation and tagging |
| GB201217772D0 (en) | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Sequencing method |
| WO2014062717A1 (en) | 2012-10-15 | 2014-04-24 | Life Technologies Corporation | Compositions, methods, systems and kits for target nucleic acid enrichment |
| US10155942B2 (en) | 2012-11-05 | 2018-12-18 | Takara Bio Usa, Inc. | Barcoding nucleic acids |
| US20140127688A1 (en) | 2012-11-07 | 2014-05-08 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| CN105026576A (zh) | 2012-12-03 | 2015-11-04 | 以琳生物药物有限公司 | 单链多核苷酸扩增方法 |
| WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2752664A1 (en) | 2013-01-07 | 2014-07-09 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Label-free method for the detection of analytes |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| KR101984699B1 (ko) | 2013-01-24 | 2019-05-31 | 삼성전자주식회사 | 핵산 분석용 미세 유체 시스템 |
| DK2954065T3 (da) | 2013-02-08 | 2021-09-06 | 10X Genomics Inc | Opdeling og bearbejdning af analytter og andre arter |
| EP2964787B1 (en) | 2013-03-08 | 2018-09-12 | Bio-Rad Laboratories, Inc. | Compositions, methods and systems for polymerase chain reaction assays |
| DK3553175T3 (da) | 2013-03-13 | 2021-08-23 | Illumina Inc | Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek |
| US9328382B2 (en) | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| WO2014144495A1 (en) | 2013-03-15 | 2014-09-18 | Abvitro, Inc. | Single cell bar-coding for antibody discovery |
| US20140274729A1 (en) | 2013-03-15 | 2014-09-18 | Nugen Technologies, Inc. | Methods, compositions and kits for generation of stranded rna or dna libraries |
| CN105283558B (zh) | 2013-03-15 | 2019-05-10 | 西格尼斯生物技术有限责任公司 | 使用热稳定的TthPrimPol扩增和测序的方法 |
| CN105431575B (zh) | 2013-05-09 | 2017-08-29 | 生物辐射实验室股份有限公司 | 磁性免疫数字pcr试验 |
| EP4617376A3 (en) | 2013-05-23 | 2025-12-10 | The Board of Trustees of the Leland Stanford Junior University | Transposition into native chromatin for personal epigenomics |
| US20160122753A1 (en) | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| KR20240172759A (ko) | 2013-06-17 | 2024-12-10 | 더 브로드 인스티튜트, 인코퍼레이티드 | 간의 표적화 및 치료를 위한 ＣＲＩＳＰＲＣａｓ 시스템, 벡터 및 조성물의 전달 및 용도 |
| US20160208323A1 (en) | 2013-06-21 | 2016-07-21 | The Broad Institute, Inc. | Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing |
| EP3019618B1 (en) | 2013-07-12 | 2018-10-31 | University of South Alabama | Minimal piggybac vectors for genome integration |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
| CA2959978A1 (en) | 2013-09-24 | 2015-04-02 | The Regents Of The University Of California | Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| WO2015053943A1 (en) | 2013-10-09 | 2015-04-16 | Stc.Unm | Synthetic long read dna sequencing |
| WO2015065924A2 (en) | 2013-10-28 | 2015-05-07 | Massachusetts Institute Of Technology | Hydrogel microstructures with immiscible fluid isolation for small reaction volumes |
| CN106460033B (zh) | 2013-12-30 | 2021-12-24 | 阿特雷卡公司 | 使用核酸条形码分析与单细胞缔合的核酸 |
| EP3102691B1 (en) | 2014-02-03 | 2019-09-11 | Thermo Fisher Scientific Baltics UAB | Method for controlled dna fragmentation |
| CN105531408B (zh) | 2014-02-13 | 2019-09-10 | 生物辐射实验室股份有限公司 | 染色体构象划分产物捕获 |
| JP6996844B2 (ja) | 2014-02-27 | 2022-02-03 | ジャンプコード ゲノミクス，インク． | 体細胞可動要素の分析のための方法、およびその使用 |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| SG11201608585RA (en) | 2014-04-15 | 2016-11-29 | Illumina Inc | Modified transposases for improved insertion sequence bias and increased dna input tolerance |
| LT3134536T (lt) | 2014-04-21 | 2020-05-11 | President And Fellows Of Harvard College | Sistemos ir būdai, skirti nukleorūgščių brūkšniniam kodavimui |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| WO2015179706A1 (en) | 2014-05-23 | 2015-11-26 | Fluidigm Corporation | Haploidome determination by digitized transposons |
| WO2015191877A1 (en) | 2014-06-11 | 2015-12-17 | Life Technologies Corporation | Systems and methods for substrate enrichment |
| EP3161157B1 (en) | 2014-06-24 | 2024-03-27 | Bio-Rad Laboratories, Inc. | Digital pcr barcoding |
| CN106575322B (zh) | 2014-06-26 | 2019-06-18 | 10X基因组学有限公司 | 核酸序列装配的方法和系统 |
| US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| MX2016016904A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Analisis de secuencias de acidos nucleicos. |
| KR102531677B1 (ko) | 2014-06-26 | 2023-05-10 | 10엑스 제노믹스, 인크. | 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법 |
| CN106574298A (zh) | 2014-06-26 | 2017-04-19 | 10X基因组学有限公司 | 用于样品分析的方法和组合物 |
| US10697007B2 (en) * | 2014-06-27 | 2020-06-30 | The Regents Of The University Of California | PCR-activated sorting (PAS) |
| US20160024558A1 (en) | 2014-07-23 | 2016-01-28 | 10X Genomics, Inc. | Nucleic acid binding proteins and uses thereof |
| EP4105337A1 (en) | 2014-09-09 | 2022-12-21 | The Broad Institute, Inc. | A droplet-based method and apparatus for composite single-cell nucleic acid analysis |
| WO2016044227A1 (en) | 2014-09-15 | 2016-03-24 | Abvitro, Inc. | High-throughput nucleotide library sequencing |
| AU2015331739B2 (en) | 2014-10-17 | 2021-12-02 | Illumina Cambridge Limited | Contiguity preserving transposition |
| KR20170073667A (ko) | 2014-10-29 | 2017-06-28 | 10엑스 제노믹스, 인크. | 표적화 핵산 서열 분석을 위한 방법 및 조성물 |
| KR102321863B1 (ko) | 2015-01-12 | 2021-11-08 | 10엑스 제노믹스, 인크. | 핵산 시퀀싱 라이브러리의 제조 방법 및 시스템 및 이를 이용하여 제조한 라이브러리 |
| JP2018508198A (ja) | 2015-02-04 | 2018-03-29 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 別個の実体におけるバーコード付加による核酸のシーケンシング |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| BR112017018054A2 (pt) * | 2015-02-24 | 2018-07-24 | 10X Genomics Inc | métodos para a cobertura de sequências de ácidos nucleicos direcionadas |
| CN107532218A (zh) | 2015-05-18 | 2018-01-02 | 10X基因组学有限公司 | 稳定化还原剂及其使用方法 |
| US20160348093A1 (en) | 2015-05-18 | 2016-12-01 | 10X Genomics, Inc. | Mobile Solid Phase Compositions for Use in Biochemical Reactions and Analyses |
| WO2016187717A1 (en) | 2015-05-26 | 2016-12-01 | Exerkine Corporation | Exosomes useful for genome editing |
| US20180087050A1 (en) | 2015-05-27 | 2018-03-29 | Jianbiao Zheng | Methods of inserting molecular barcodes |
| EP3314018A1 (en) | 2015-06-24 | 2018-05-02 | Oxford Biodynamics Limited | Detection processes using sites of chromosome interaction |
| AU2016297510B2 (en) | 2015-07-17 | 2021-09-09 | President And Fellows Of Harvard College | Methods of amplifying nucleic acid sequences |
| EP3334841B1 (en) | 2015-08-12 | 2019-10-30 | CeMM - Forschungszentrum für Molekulare Medizin GmbH | Methods for studying nucleic acids |
| WO2017053905A1 (en) | 2015-09-24 | 2017-03-30 | Abvitro Llc | Affinity-oligonucleotide conjugates and uses thereof |
| WO2017075265A1 (en) | 2015-10-28 | 2017-05-04 | The Broad Institute, Inc. | Multiplex analysis of single cell constituents |
| CN108350499B (zh) | 2015-11-19 | 2022-05-13 | 10X基因组学有限公司 | 可转化标记组合物、方法及结合其的过程 |
| WO2017139690A1 (en) | 2016-02-11 | 2017-08-17 | 10X Genomics, Inc. | Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes |
| CN109196115A (zh) | 2016-03-01 | 2019-01-11 | 通用测序技术公司 | 跟踪核酸靶标来源以用于核酸测序的方法和试剂盒 |
| CN109072206A (zh) | 2016-03-10 | 2018-12-21 | 斯坦福大学托管董事会 | 转座酶介导的对可接近基因组的成像 |
| WO2017180420A1 (en) | 2016-04-11 | 2017-10-19 | Board Of Regents, The University Of Texas System | Methods and compositions for detecting single t cell receptor affinity and sequence |
| GB2569252A (en) | 2016-08-31 | 2019-06-12 | Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| US11021738B2 (en) | 2016-12-19 | 2021-06-01 | Bio-Rad Laboratories, Inc. | Droplet tagging contiguity preserved tagmented DNA |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| US10914729B2 (en) | 2017-05-22 | 2021-02-09 | The Trustees Of Princeton University | Methods for detecting protein binding sequences and tagging nucleic acids |
| US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN108336542B (zh) | 2017-06-23 | 2020-02-21 | 番禺得意精密电子工业有限公司 | 电连接器 |
| WO2019084328A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS FOR PREPARING NUCLEIC ACID MOLECULES |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
-
2016
- 2016-02-24 BR BR112017018054A patent/BR112017018054A2/pt not_active Application Discontinuation
- 2016-02-24 EP EP21170986.0A patent/EP3936619A1/en not_active Withdrawn
- 2016-02-24 MX MX2017010857A patent/MX2017010857A/es unknown
- 2016-02-24 WO PCT/US2016/019382 patent/WO2016138148A1/en not_active Ceased
- 2016-02-24 JP JP2017543973A patent/JP2018505688A/ja active Pending
- 2016-02-24 KR KR1020177026877A patent/KR20170119710A/ko not_active Withdrawn
- 2016-02-24 CA CA2975958A patent/CA2975958A1/en not_active Abandoned
- 2016-02-24 EP EP16710366.2A patent/EP3262188B1/en active Active
- 2016-02-24 CN CN201680011692.7A patent/CN107532202A/zh active Pending
- 2016-02-24 US US15/052,612 patent/US11274343B2/en active Active
- 2016-02-24 AU AU2016222719A patent/AU2016222719B2/en active Active
- 2016-02-24 CN CN202210652941.8A patent/CN115651972A/zh active Pending
-
2017
- 2017-08-23 IL IL254120A patent/IL254120A0/en unknown
-
2022
- 2022-02-02 US US17/591,480 patent/US20220403464A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014210353A2 (en) * | 2013-06-27 | 2014-12-31 | 10X Technologies, Inc. | Compositions and methods for sample processing |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2016222719B2 (en) | 2022-03-31 |
| IL254120A0 (en) | 2017-10-31 |
| MX2017010857A (es) | 2017-12-11 |
| US11274343B2 (en) | 2022-03-15 |
| BR112017018054A2 (pt) | 2018-07-24 |
| CN107532202A (zh) | 2018-01-02 |
| KR20170119710A (ko) | 2017-10-27 |
| US20220403464A1 (en) | 2022-12-22 |
| EP3262188B1 (en) | 2021-05-05 |
| CA2975958A1 (en) | 2016-09-01 |
| EP3262188A1 (en) | 2018-01-03 |
| CN115651972A (zh) | 2023-01-31 |
| AU2016222719A1 (en) | 2017-09-07 |
| WO2016138148A1 (en) | 2016-09-01 |
| US20160281160A1 (en) | 2016-09-29 |
| EP3936619A1 (en) | 2022-01-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220403464A1 (en) | Methods and Compositions for Targeted Nucleic Acid Sequence Coverage | |
| US20240035066A1 (en) | Methods and compositions for targeted nucleic acid sequencing | |
| CN108431232B (zh) | 用于核酸分析的方法和组合物 | |
| HK40084565A (en) | Methods and compositions for nucleic acid analysis | |
| HK40059275B (en) | Methods and compositions for nucleic acid analysis | |
| HK40059275A (en) | Methods and compositions for nucleic acid analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190219 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190219 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20191113 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20191113 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20200213 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20200413 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200513 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20201029 |