JP2018141017A5 - - Google Patents
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- Publication number
- JP2018141017A5 JP2018141017A5 JP2018107188A JP2018107188A JP2018141017A5 JP 2018141017 A5 JP2018141017 A5 JP 2018141017A5 JP 2018107188 A JP2018107188 A JP 2018107188A JP 2018107188 A JP2018107188 A JP 2018107188A JP 2018141017 A5 JP2018141017 A5 JP 2018141017A5
- Authority
- JP
- Japan
- Prior art keywords
- intein
- fusion protein
- polypeptide
- solid support
- target molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462074494P | 2014-11-03 | 2014-11-03 | |
| US62/074,494 | 2014-11-03 | ||
| US201562209010P | 2015-08-24 | 2015-08-24 | |
| US62/209,010 | 2015-08-24 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017521137A Division JP6349462B2 (ja) | 2014-11-03 | 2015-10-23 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020136598A Division JP7054722B2 (ja) | 2014-11-03 | 2020-08-13 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018141017A JP2018141017A (ja) | 2018-09-13 |
| JP2018141017A5 true JP2018141017A5 (https=) | 2019-06-27 |
| JP7058560B2 JP7058560B2 (ja) | 2022-04-22 |
Family
ID=54397030
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017521137A Active JP6349462B2 (ja) | 2014-11-03 | 2015-10-23 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
| JP2018107188A Active JP7058560B2 (ja) | 2014-11-03 | 2018-06-04 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
| JP2020136598A Active JP7054722B2 (ja) | 2014-11-03 | 2020-08-13 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017521137A Active JP6349462B2 (ja) | 2014-11-03 | 2015-10-23 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020136598A Active JP7054722B2 (ja) | 2014-11-03 | 2020-08-13 | 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US10308679B2 (https=) |
| EP (2) | EP3543335A3 (https=) |
| JP (3) | JP6349462B2 (https=) |
| KR (3) | KR102105352B1 (https=) |
| CN (1) | CN107406514B (https=) |
| CA (1) | CA2964119C (https=) |
| ES (1) | ES2742199T3 (https=) |
| PL (1) | PL3215614T3 (https=) |
| SG (2) | SG11201701965VA (https=) |
| SI (1) | SI3215614T1 (https=) |
| WO (1) | WO2016073228A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107406514B (zh) | 2014-11-03 | 2021-11-30 | 默克专利有限公司 | 可溶内含肽融合蛋白以及用于纯化生物分子的方法 |
| KR102000490B1 (ko) * | 2018-01-12 | 2019-10-01 | 전남대학교산학협력단 | 가용성이 개선된 살모넬라균 편모 유래 플라젤린 단백질 발현 형질전환체, 그 제조방법 및 용도 |
| CN113195516B (zh) * | 2018-09-11 | 2025-07-15 | 纳诺泰克生物技术公司 | 由特异性结合物识别的表位标签 |
| US11136360B2 (en) | 2018-12-19 | 2021-10-05 | Tsinghua University | Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification |
| KR102129377B1 (ko) * | 2019-02-21 | 2020-07-02 | 성균관대학교산학협력단 | 특정 특성이 발현된 단백질을 암호화하는 플라스미드를 선별하는 유도 플라스미드 디스플레이 시스템 |
| CN110201419B (zh) * | 2019-05-09 | 2021-01-19 | 西安交通大学 | 一种以聚乙烯醇微球为载体的细胞膜色谱柱及其制备方法 |
| GB201917046D0 (en) | 2019-11-22 | 2020-01-08 | Ge Healthcare Bioprocess R&D Ab | Improved protein production |
| CN115279779A (zh) * | 2020-03-25 | 2022-11-01 | 默克专利股份公司 | 用于蛋白质纯化的内含肽结合树脂的再使用 |
| AU2021268757A1 (en) * | 2020-05-07 | 2022-12-08 | Adaptvac Aps | Peptide tags and binding partners |
| KR20230077744A (ko) * | 2020-09-28 | 2023-06-01 | 메르크 파텐트 게엠베하 | 인테인 복합체로부터의 표적 방출 방법 |
| CA3216901A1 (en) | 2021-05-12 | 2022-11-17 | Cytiva Bioprocess R&D Ab | Improved protein purification |
| CN116041451B (zh) * | 2022-08-15 | 2023-08-15 | 广州市乾相生物科技有限公司 | 一种内含肽变体及其在生物法制备蓝铜胜肽中的应用 |
| CN116731126B (zh) * | 2023-01-30 | 2024-02-23 | 态创生物科技(广州)有限公司 | 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法 |
| CN117964708A (zh) * | 2023-11-14 | 2024-05-03 | 中国科学院微生物研究所 | 一种内含肽及其突变体的应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6399570B1 (en) | 1999-02-05 | 2002-06-04 | Agennix, Inc. | Antimicrobial/endotoxin neutralizing polypeptide |
| JP3952347B2 (ja) * | 1999-04-16 | 2007-08-01 | 株式会社リコー | 搬送装置 |
| FR2803913B1 (fr) * | 2000-01-13 | 2002-08-16 | Pasteur Sanofi Diagnostics | Procede d'immobilisation de reactif(s) affin(s) sur phase solide hydrophobe |
| WO2002064834A1 (en) * | 2001-01-04 | 2002-08-22 | Myriad Genetics, Inc. | Novel two-hybrid system and use thereof |
| US8362217B2 (en) | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
| NZ577933A (en) | 2007-01-22 | 2011-12-22 | Genentech Inc | Polyelectrolyte precipitation and purification of antibodies |
| SG149759A1 (en) * | 2007-07-10 | 2009-02-27 | Millipore Corp | Media for affinity chromatography |
| CA2754851A1 (en) | 2009-03-10 | 2010-09-16 | Dsm Ip Assets B.V. | Method for improving the yield of a polypeptide |
| CN101884910A (zh) | 2009-05-12 | 2010-11-17 | 南京大学 | 利用具有反式剪接功能的蛋白质内含子制备重组多肽 |
| EP2761006B1 (en) | 2011-09-28 | 2016-12-14 | Zera Intein Protein Solutions, S.L. | Split inteins and uses thereof |
| DK3431497T3 (da) * | 2012-06-27 | 2022-10-10 | Univ Princeton | Spaltede inteiner, konjugater og anvendelser deraf |
| EP2943573A1 (en) | 2013-01-11 | 2015-11-18 | The Texas A&M University System | Intein mediated purification of protein |
| US9670257B2 (en) * | 2013-05-31 | 2017-06-06 | Novo Nordisk A/S | Methods for producing peptides using engineered inteins |
| CN104387473B (zh) | 2014-10-27 | 2017-10-10 | 郑州大学 | 用于非酶切非色谱纯化方法原核表达融合蛋白Prx的类弹性蛋白多肽ELP |
| WO2016069774A1 (en) * | 2014-10-28 | 2016-05-06 | Agrivida, Inc. | Methods and compositions for stabilizing trans-splicing intein modified proteases |
| CN107406514B (zh) | 2014-11-03 | 2021-11-30 | 默克专利有限公司 | 可溶内含肽融合蛋白以及用于纯化生物分子的方法 |
-
2015
- 2015-10-23 CN CN201580059731.6A patent/CN107406514B/zh active Active
- 2015-10-23 KR KR1020197018450A patent/KR102105352B1/ko active Active
- 2015-10-23 EP EP19169588.1A patent/EP3543335A3/en not_active Withdrawn
- 2015-10-23 WO PCT/US2015/057125 patent/WO2016073228A1/en not_active Ceased
- 2015-10-23 KR KR1020177014681A patent/KR101891455B1/ko active Active
- 2015-10-23 KR KR1020187023540A patent/KR101996774B1/ko active Active
- 2015-10-23 SG SG11201701965VA patent/SG11201701965VA/en unknown
- 2015-10-23 PL PL15790411T patent/PL3215614T3/pl unknown
- 2015-10-23 EP EP15790411.1A patent/EP3215614B1/en active Active
- 2015-10-23 US US15/511,908 patent/US10308679B2/en active Active
- 2015-10-23 JP JP2017521137A patent/JP6349462B2/ja active Active
- 2015-10-23 CA CA2964119A patent/CA2964119C/en active Active
- 2015-10-23 SG SG10201801320UA patent/SG10201801320UA/en unknown
- 2015-10-23 SI SI201530827T patent/SI3215614T1/sl unknown
- 2015-10-23 ES ES15790411T patent/ES2742199T3/es active Active
-
2018
- 2018-06-04 JP JP2018107188A patent/JP7058560B2/ja active Active
-
2019
- 2019-04-18 US US16/388,193 patent/US11326154B2/en active Active
-
2020
- 2020-08-13 JP JP2020136598A patent/JP7054722B2/ja active Active
-
2022
- 2022-04-13 US US17/659,021 patent/US11926854B2/en active Active
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