SG11201701965VA - Soluble intein fusion proteins and methods for purifying biomolecules - Google Patents
Soluble intein fusion proteins and methods for purifying biomoleculesInfo
- Publication number
- SG11201701965VA SG11201701965VA SG11201701965VA SG11201701965VA SG11201701965VA SG 11201701965V A SG11201701965V A SG 11201701965VA SG 11201701965V A SG11201701965V A SG 11201701965VA SG 11201701965V A SG11201701965V A SG 11201701965VA SG 11201701965V A SG11201701965V A SG 11201701965VA
- Authority
- SG
- Singapore
- Prior art keywords
- soluble
- methods
- fusion proteins
- intein fusion
- purifying biomolecules
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462074494P | 2014-11-03 | 2014-11-03 | |
| US201562209010P | 2015-08-24 | 2015-08-24 | |
| PCT/US2015/057125 WO2016073228A1 (en) | 2014-11-03 | 2015-10-23 | Soluble intein fusion proteins and methods for purifying biomolecules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201701965VA true SG11201701965VA (en) | 2017-05-30 |
Family
ID=54397030
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201801320UA SG10201801320UA (en) | 2014-11-03 | 2015-10-23 | Soluble intein fusion proteins and methods for purifying biomolecules |
| SG11201701965VA SG11201701965VA (en) | 2014-11-03 | 2015-10-23 | Soluble intein fusion proteins and methods for purifying biomolecules |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201801320UA SG10201801320UA (en) | 2014-11-03 | 2015-10-23 | Soluble intein fusion proteins and methods for purifying biomolecules |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US10308679B2 (en) |
| EP (2) | EP3215614B1 (en) |
| JP (3) | JP6349462B2 (en) |
| KR (3) | KR101891455B1 (en) |
| CN (1) | CN107406514B (en) |
| CA (1) | CA2964119C (en) |
| ES (1) | ES2742199T3 (en) |
| PL (1) | PL3215614T3 (en) |
| SG (2) | SG10201801320UA (en) |
| SI (1) | SI3215614T1 (en) |
| WO (1) | WO2016073228A1 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101891455B1 (en) | 2014-11-03 | 2018-08-27 | 메르크 파텐트 게엠베하 | Soluble intein fusion proteins and methods for purifying biomolecules |
| KR102000490B1 (en) * | 2018-01-12 | 2019-10-01 | 전남대학교산학협력단 | Transformants for expressing flagellin protein derived from Salmonella sp. with improved solubility, preparation method and use thereof |
| WO2020053239A1 (en) * | 2018-09-11 | 2020-03-19 | Nanotag Biotechnologies Gmbh | Epitope tags recognized by specific binders |
| US11136360B2 (en) | 2018-12-19 | 2021-10-05 | Tsinghua University | Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification |
| KR102129377B1 (en) * | 2019-02-21 | 2020-07-02 | 성균관대학교산학협력단 | An inductive plasmid display system for selecting a plasmid encoding a protein with specific properties expressed |
| CN110201419B (en) * | 2019-05-09 | 2021-01-19 | 西安交通大学 | Cell membrane chromatographic column with polyvinyl alcohol microspheres as carrier and preparation method thereof |
| GB201917046D0 (en) | 2019-11-22 | 2020-01-08 | Ge Healthcare Bioprocess R&D Ab | Improved protein production |
| EP4337670A1 (en) | 2021-05-12 | 2024-03-20 | Cytiva BioProcess R&D AB | Improved protein purification |
| CN116041451B (en) * | 2022-08-15 | 2023-08-15 | 广州市乾相生物科技有限公司 | Intein variant and application thereof in preparation of blue copper peptide by biological method |
| CN116731126B (en) * | 2023-01-30 | 2024-02-23 | 态创生物科技(广州)有限公司 | Intein ChiATP, intein ChiATP-dipeptide-2 fusion protein and dipeptide-2 expression method |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6399570B1 (en) | 1999-02-05 | 2002-06-04 | Agennix, Inc. | Antimicrobial/endotoxin neutralizing polypeptide |
| JP3952347B2 (en) * | 1999-04-16 | 2007-08-01 | 株式会社リコー | Transport device |
| FR2803913B1 (en) * | 2000-01-13 | 2002-08-16 | Pasteur Sanofi Diagnostics | METHOD OF IMMOBILIZING AFFIN (A) REAGENT (S) ON A HYDROPHOBIC SOLID PHASE |
| US6828112B2 (en) | 2001-01-04 | 2004-12-07 | Myriad Genetics, Inc. | Method of detecting protein-protein interactions |
| US8362217B2 (en) | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
| AU2008209404B2 (en) | 2007-01-22 | 2012-08-16 | Genentech, Inc. | Polyelectrolyte precipitation and purification of antibodies |
| SG149759A1 (en) * | 2007-07-10 | 2009-02-27 | Millipore Corp | Media for affinity chromatography |
| CN102741421B (en) | 2009-03-10 | 2015-03-25 | 帝斯曼知识产权资产管理有限公司 | Method for improving the yield of a polypeptide |
| CN101884910A (en) | 2009-05-12 | 2010-11-17 | 南京大学 | Preparation of recombinant polypeptide by intein with trans-splicing function |
| ES2618632T3 (en) * | 2011-09-28 | 2017-06-21 | Zera Intein Protein Solutions, S.L. | Divided Inteins and Uses of These |
| EP3431497B1 (en) * | 2012-06-27 | 2022-07-27 | The Trustees of Princeton University | Split inteins, conjugates and uses thereof |
| US10087213B2 (en) | 2013-01-11 | 2018-10-02 | The Texas A&M University System | Intein mediated purification of protein |
| WO2014191455A1 (en) | 2013-05-31 | 2014-12-04 | Novo Nordisk A/S | Methods for producing peptides using engineered inteins |
| CN104387473B (en) | 2014-10-27 | 2017-10-10 | 郑州大学 | Class elastin polypeptide ELP for the non-chromatographic purification method prokaryotic expression fusion protein Prx of non-digestion |
| DK3212778T3 (en) * | 2014-10-28 | 2019-11-04 | Agrivida Inc | Methods and compositions for stabilizing trans-splicing of intein-modified proteases |
| KR101891455B1 (en) | 2014-11-03 | 2018-08-27 | 메르크 파텐트 게엠베하 | Soluble intein fusion proteins and methods for purifying biomolecules |
-
2015
- 2015-10-23 KR KR1020177014681A patent/KR101891455B1/en active Application Filing
- 2015-10-23 US US15/511,908 patent/US10308679B2/en active Active
- 2015-10-23 EP EP15790411.1A patent/EP3215614B1/en active Active
- 2015-10-23 PL PL15790411T patent/PL3215614T3/en unknown
- 2015-10-23 EP EP19169588.1A patent/EP3543335A3/en active Pending
- 2015-10-23 KR KR1020197018450A patent/KR102105352B1/en active IP Right Grant
- 2015-10-23 CN CN201580059731.6A patent/CN107406514B/en active Active
- 2015-10-23 ES ES15790411T patent/ES2742199T3/en active Active
- 2015-10-23 WO PCT/US2015/057125 patent/WO2016073228A1/en active Application Filing
- 2015-10-23 SI SI201530827T patent/SI3215614T1/en unknown
- 2015-10-23 JP JP2017521137A patent/JP6349462B2/en active Active
- 2015-10-23 CA CA2964119A patent/CA2964119C/en active Active
- 2015-10-23 KR KR1020187023540A patent/KR101996774B1/en active Application Filing
- 2015-10-23 SG SG10201801320UA patent/SG10201801320UA/en unknown
- 2015-10-23 SG SG11201701965VA patent/SG11201701965VA/en unknown
-
2018
- 2018-06-04 JP JP2018107188A patent/JP7058560B2/en active Active
-
2019
- 2019-04-18 US US16/388,193 patent/US11326154B2/en active Active
-
2020
- 2020-08-13 JP JP2020136598A patent/JP7054722B2/en active Active
-
2022
- 2022-04-13 US US17/659,021 patent/US11926854B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| SI3215614T1 (en) | 2019-11-29 |
| JP7058560B2 (en) | 2022-04-22 |
| CA2964119C (en) | 2022-10-18 |
| EP3215614B1 (en) | 2019-05-29 |
| KR102105352B1 (en) | 2020-04-29 |
| PL3215614T3 (en) | 2019-12-31 |
| SG10201801320UA (en) | 2018-04-27 |
| US20190241609A1 (en) | 2019-08-08 |
| JP7054722B2 (en) | 2022-04-14 |
| US20170291919A1 (en) | 2017-10-12 |
| KR20170067901A (en) | 2017-06-16 |
| CA2964119A1 (en) | 2016-05-12 |
| CN107406514B (en) | 2021-11-30 |
| JP6349462B2 (en) | 2018-06-27 |
| US11926854B2 (en) | 2024-03-12 |
| JP2017533701A (en) | 2017-11-16 |
| US20220267750A1 (en) | 2022-08-25 |
| KR101891455B1 (en) | 2018-08-27 |
| EP3543335A3 (en) | 2019-11-27 |
| ES2742199T3 (en) | 2020-02-13 |
| WO2016073228A1 (en) | 2016-05-12 |
| EP3215614A1 (en) | 2017-09-13 |
| CN107406514A (en) | 2017-11-28 |
| KR20180095125A (en) | 2018-08-24 |
| EP3543335A2 (en) | 2019-09-25 |
| KR20190077620A (en) | 2019-07-03 |
| JP2018141017A (en) | 2018-09-13 |
| US11326154B2 (en) | 2022-05-10 |
| JP2020180162A (en) | 2020-11-05 |
| KR101996774B1 (en) | 2019-07-04 |
| US10308679B2 (en) | 2019-06-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| HRP20190028T1 (en) | Interleukin-2 fusion proteins and uses thereof | |
| HK1244019A1 (en) | Fusion proteins | |
| ZA201701810B (en) | Sirp-alpha immunoglobulin fusion proteins | |
| IL251194A0 (en) | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria | |
| SG11201701965VA (en) | Soluble intein fusion proteins and methods for purifying biomolecules | |
| GB201509413D0 (en) | Fusion protein | |
| GB201509062D0 (en) | OX40L fusion proteins and uses thereof | |
| HK1232136A1 (en) | P97-ids fusion proteins p97-ids | |
| PT3201336T (en) | Methods for extracting and purifying non-denatured proteins | |
| IL265016B (en) | Methods for purifying antibodies | |
| GB201504691D0 (en) | Fusion protein | |
| HUE057808T2 (en) | Protein recovery | |
| GB201503218D0 (en) | Particles comprising fusion proteins | |
| HK1257937A1 (en) | Fusion protein | |
| EP3139955A4 (en) | Fusion proteins for treating cancer and related methods | |
| EP3237452A4 (en) | Fusion proteins and methods thereof | |
| SG11201606501PA (en) | Nanobody-fluorescent protein fusion | |
| GB201602850D0 (en) | Fusion proteins |