CN107406514B - 可溶内含肽融合蛋白以及用于纯化生物分子的方法 - Google Patents

可溶内含肽融合蛋白以及用于纯化生物分子的方法 Download PDF

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CN107406514B
CN107406514B CN201580059731.6A CN201580059731A CN107406514B CN 107406514 B CN107406514 B CN 107406514B CN 201580059731 A CN201580059731 A CN 201580059731A CN 107406514 B CN107406514 B CN 107406514B
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intein
fusion protein
amino acid
acid sequence
polypeptide
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CN107406514A (zh
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M·齐尔曼
J·奥兰多
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Merck Patent GmbH
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Merck Patent GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN201580059731.6A 2014-11-03 2015-10-23 可溶内含肽融合蛋白以及用于纯化生物分子的方法 Active CN107406514B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462074494P 2014-11-03 2014-11-03
US62/074,494 2014-11-03
US201562209010P 2015-08-24 2015-08-24
US62/209,010 2015-08-24
PCT/US2015/057125 WO2016073228A1 (en) 2014-11-03 2015-10-23 Soluble intein fusion proteins and methods for purifying biomolecules

Publications (2)

Publication Number Publication Date
CN107406514A CN107406514A (zh) 2017-11-28
CN107406514B true CN107406514B (zh) 2021-11-30

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US (3) US10308679B2 (https=)
EP (2) EP3543335A3 (https=)
JP (3) JP6349462B2 (https=)
KR (3) KR102105352B1 (https=)
CN (1) CN107406514B (https=)
CA (1) CA2964119C (https=)
ES (1) ES2742199T3 (https=)
PL (1) PL3215614T3 (https=)
SG (2) SG11201701965VA (https=)
SI (1) SI3215614T1 (https=)
WO (1) WO2016073228A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107406514B (zh) 2014-11-03 2021-11-30 默克专利有限公司 可溶内含肽融合蛋白以及用于纯化生物分子的方法
KR102000490B1 (ko) * 2018-01-12 2019-10-01 전남대학교산학협력단 가용성이 개선된 살모넬라균 편모 유래 플라젤린 단백질 발현 형질전환체, 그 제조방법 및 용도
CN113195516B (zh) * 2018-09-11 2025-07-15 纳诺泰克生物技术公司 由特异性结合物识别的表位标签
US11136360B2 (en) 2018-12-19 2021-10-05 Tsinghua University Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification
KR102129377B1 (ko) * 2019-02-21 2020-07-02 성균관대학교산학협력단 특정 특성이 발현된 단백질을 암호화하는 플라스미드를 선별하는 유도 플라스미드 디스플레이 시스템
CN110201419B (zh) * 2019-05-09 2021-01-19 西安交通大学 一种以聚乙烯醇微球为载体的细胞膜色谱柱及其制备方法
GB201917046D0 (en) 2019-11-22 2020-01-08 Ge Healthcare Bioprocess R&D Ab Improved protein production
CN115279779A (zh) * 2020-03-25 2022-11-01 默克专利股份公司 用于蛋白质纯化的内含肽结合树脂的再使用
AU2021268757A1 (en) * 2020-05-07 2022-12-08 Adaptvac Aps Peptide tags and binding partners
KR20230077744A (ko) * 2020-09-28 2023-06-01 메르크 파텐트 게엠베하 인테인 복합체로부터의 표적 방출 방법
CA3216901A1 (en) 2021-05-12 2022-11-17 Cytiva Bioprocess R&D Ab Improved protein purification
CN116041451B (zh) * 2022-08-15 2023-08-15 广州市乾相生物科技有限公司 一种内含肽变体及其在生物法制备蓝铜胜肽中的应用
CN116731126B (zh) * 2023-01-30 2024-02-23 态创生物科技(广州)有限公司 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法
CN117964708A (zh) * 2023-11-14 2024-05-03 中国科学院微生物研究所 一种内含肽及其突变体的应用

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KR20170067901A (ko) 2017-06-16
EP3215614B1 (en) 2019-05-29
EP3543335A2 (en) 2019-09-25
KR102105352B1 (ko) 2020-04-29
SG11201701965VA (en) 2017-05-30
KR20190077620A (ko) 2019-07-03
US11926854B2 (en) 2024-03-12
US20170291919A1 (en) 2017-10-12
KR101996774B1 (ko) 2019-07-04
PL3215614T3 (pl) 2019-12-31
EP3215614A1 (en) 2017-09-13
US11326154B2 (en) 2022-05-10
JP6349462B2 (ja) 2018-06-27
JP7054722B2 (ja) 2022-04-14
WO2016073228A1 (en) 2016-05-12
US10308679B2 (en) 2019-06-04
KR101891455B1 (ko) 2018-08-27
JP7058560B2 (ja) 2022-04-22
SG10201801320UA (en) 2018-04-27
CA2964119C (en) 2022-10-18
JP2017533701A (ja) 2017-11-16
KR20180095125A (ko) 2018-08-24
JP2018141017A (ja) 2018-09-13
US20190241609A1 (en) 2019-08-08
ES2742199T3 (es) 2020-02-13
SI3215614T1 (sl) 2019-11-29
US20220267750A1 (en) 2022-08-25
EP3543335A3 (en) 2019-11-27
CN107406514A (zh) 2017-11-28
CA2964119A1 (en) 2016-05-12
JP2020180162A (ja) 2020-11-05

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