CA2964119C - Soluble intein fusion proteins and methods for purifying biomolecules - Google Patents
Soluble intein fusion proteins and methods for purifying biomolecules Download PDFInfo
- Publication number
- CA2964119C CA2964119C CA2964119A CA2964119A CA2964119C CA 2964119 C CA2964119 C CA 2964119C CA 2964119 A CA2964119 A CA 2964119A CA 2964119 A CA2964119 A CA 2964119A CA 2964119 C CA2964119 C CA 2964119C
- Authority
- CA
- Canada
- Prior art keywords
- intein
- polypeptide
- fusion protein
- solubilization
- affinity chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462074494P | 2014-11-03 | 2014-11-03 | |
| US62/074,494 | 2014-11-03 | ||
| US201562209010P | 2015-08-24 | 2015-08-24 | |
| US62/209,010 | 2015-08-24 | ||
| PCT/US2015/057125 WO2016073228A1 (en) | 2014-11-03 | 2015-10-23 | Soluble intein fusion proteins and methods for purifying biomolecules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2964119A1 CA2964119A1 (en) | 2016-05-12 |
| CA2964119C true CA2964119C (en) | 2022-10-18 |
Family
ID=54397030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2964119A Active CA2964119C (en) | 2014-11-03 | 2015-10-23 | Soluble intein fusion proteins and methods for purifying biomolecules |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US10308679B2 (https=) |
| EP (2) | EP3543335A3 (https=) |
| JP (3) | JP6349462B2 (https=) |
| KR (3) | KR102105352B1 (https=) |
| CN (1) | CN107406514B (https=) |
| CA (1) | CA2964119C (https=) |
| ES (1) | ES2742199T3 (https=) |
| PL (1) | PL3215614T3 (https=) |
| SG (2) | SG11201701965VA (https=) |
| SI (1) | SI3215614T1 (https=) |
| WO (1) | WO2016073228A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107406514B (zh) | 2014-11-03 | 2021-11-30 | 默克专利有限公司 | 可溶内含肽融合蛋白以及用于纯化生物分子的方法 |
| KR102000490B1 (ko) * | 2018-01-12 | 2019-10-01 | 전남대학교산학협력단 | 가용성이 개선된 살모넬라균 편모 유래 플라젤린 단백질 발현 형질전환체, 그 제조방법 및 용도 |
| CN113195516B (zh) * | 2018-09-11 | 2025-07-15 | 纳诺泰克生物技术公司 | 由特异性结合物识别的表位标签 |
| US11136360B2 (en) | 2018-12-19 | 2021-10-05 | Tsinghua University | Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification |
| KR102129377B1 (ko) * | 2019-02-21 | 2020-07-02 | 성균관대학교산학협력단 | 특정 특성이 발현된 단백질을 암호화하는 플라스미드를 선별하는 유도 플라스미드 디스플레이 시스템 |
| CN110201419B (zh) * | 2019-05-09 | 2021-01-19 | 西安交通大学 | 一种以聚乙烯醇微球为载体的细胞膜色谱柱及其制备方法 |
| GB201917046D0 (en) | 2019-11-22 | 2020-01-08 | Ge Healthcare Bioprocess R&D Ab | Improved protein production |
| CN115279779A (zh) * | 2020-03-25 | 2022-11-01 | 默克专利股份公司 | 用于蛋白质纯化的内含肽结合树脂的再使用 |
| AU2021268757A1 (en) * | 2020-05-07 | 2022-12-08 | Adaptvac Aps | Peptide tags and binding partners |
| KR20230077744A (ko) * | 2020-09-28 | 2023-06-01 | 메르크 파텐트 게엠베하 | 인테인 복합체로부터의 표적 방출 방법 |
| CA3216901A1 (en) | 2021-05-12 | 2022-11-17 | Cytiva Bioprocess R&D Ab | Improved protein purification |
| CN116041451B (zh) * | 2022-08-15 | 2023-08-15 | 广州市乾相生物科技有限公司 | 一种内含肽变体及其在生物法制备蓝铜胜肽中的应用 |
| CN116731126B (zh) * | 2023-01-30 | 2024-02-23 | 态创生物科技(广州)有限公司 | 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法 |
| CN117964708A (zh) * | 2023-11-14 | 2024-05-03 | 中国科学院微生物研究所 | 一种内含肽及其突变体的应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6399570B1 (en) | 1999-02-05 | 2002-06-04 | Agennix, Inc. | Antimicrobial/endotoxin neutralizing polypeptide |
| JP3952347B2 (ja) * | 1999-04-16 | 2007-08-01 | 株式会社リコー | 搬送装置 |
| FR2803913B1 (fr) * | 2000-01-13 | 2002-08-16 | Pasteur Sanofi Diagnostics | Procede d'immobilisation de reactif(s) affin(s) sur phase solide hydrophobe |
| WO2002064834A1 (en) * | 2001-01-04 | 2002-08-22 | Myriad Genetics, Inc. | Novel two-hybrid system and use thereof |
| US8362217B2 (en) | 2006-12-21 | 2013-01-29 | Emd Millipore Corporation | Purification of proteins |
| NZ577933A (en) | 2007-01-22 | 2011-12-22 | Genentech Inc | Polyelectrolyte precipitation and purification of antibodies |
| SG149759A1 (en) * | 2007-07-10 | 2009-02-27 | Millipore Corp | Media for affinity chromatography |
| CA2754851A1 (en) | 2009-03-10 | 2010-09-16 | Dsm Ip Assets B.V. | Method for improving the yield of a polypeptide |
| CN101884910A (zh) | 2009-05-12 | 2010-11-17 | 南京大学 | 利用具有反式剪接功能的蛋白质内含子制备重组多肽 |
| EP2761006B1 (en) | 2011-09-28 | 2016-12-14 | Zera Intein Protein Solutions, S.L. | Split inteins and uses thereof |
| DK3431497T3 (da) * | 2012-06-27 | 2022-10-10 | Univ Princeton | Spaltede inteiner, konjugater og anvendelser deraf |
| EP2943573A1 (en) | 2013-01-11 | 2015-11-18 | The Texas A&M University System | Intein mediated purification of protein |
| US9670257B2 (en) * | 2013-05-31 | 2017-06-06 | Novo Nordisk A/S | Methods for producing peptides using engineered inteins |
| CN104387473B (zh) | 2014-10-27 | 2017-10-10 | 郑州大学 | 用于非酶切非色谱纯化方法原核表达融合蛋白Prx的类弹性蛋白多肽ELP |
| WO2016069774A1 (en) * | 2014-10-28 | 2016-05-06 | Agrivida, Inc. | Methods and compositions for stabilizing trans-splicing intein modified proteases |
| CN107406514B (zh) | 2014-11-03 | 2021-11-30 | 默克专利有限公司 | 可溶内含肽融合蛋白以及用于纯化生物分子的方法 |
-
2015
- 2015-10-23 CN CN201580059731.6A patent/CN107406514B/zh active Active
- 2015-10-23 KR KR1020197018450A patent/KR102105352B1/ko active Active
- 2015-10-23 EP EP19169588.1A patent/EP3543335A3/en not_active Withdrawn
- 2015-10-23 WO PCT/US2015/057125 patent/WO2016073228A1/en not_active Ceased
- 2015-10-23 KR KR1020177014681A patent/KR101891455B1/ko active Active
- 2015-10-23 KR KR1020187023540A patent/KR101996774B1/ko active Active
- 2015-10-23 SG SG11201701965VA patent/SG11201701965VA/en unknown
- 2015-10-23 PL PL15790411T patent/PL3215614T3/pl unknown
- 2015-10-23 EP EP15790411.1A patent/EP3215614B1/en active Active
- 2015-10-23 US US15/511,908 patent/US10308679B2/en active Active
- 2015-10-23 JP JP2017521137A patent/JP6349462B2/ja active Active
- 2015-10-23 CA CA2964119A patent/CA2964119C/en active Active
- 2015-10-23 SG SG10201801320UA patent/SG10201801320UA/en unknown
- 2015-10-23 SI SI201530827T patent/SI3215614T1/sl unknown
- 2015-10-23 ES ES15790411T patent/ES2742199T3/es active Active
-
2018
- 2018-06-04 JP JP2018107188A patent/JP7058560B2/ja active Active
-
2019
- 2019-04-18 US US16/388,193 patent/US11326154B2/en active Active
-
2020
- 2020-08-13 JP JP2020136598A patent/JP7054722B2/ja active Active
-
2022
- 2022-04-13 US US17/659,021 patent/US11926854B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20170067901A (ko) | 2017-06-16 |
| EP3215614B1 (en) | 2019-05-29 |
| EP3543335A2 (en) | 2019-09-25 |
| KR102105352B1 (ko) | 2020-04-29 |
| SG11201701965VA (en) | 2017-05-30 |
| KR20190077620A (ko) | 2019-07-03 |
| US11926854B2 (en) | 2024-03-12 |
| US20170291919A1 (en) | 2017-10-12 |
| KR101996774B1 (ko) | 2019-07-04 |
| PL3215614T3 (pl) | 2019-12-31 |
| EP3215614A1 (en) | 2017-09-13 |
| US11326154B2 (en) | 2022-05-10 |
| JP6349462B2 (ja) | 2018-06-27 |
| JP7054722B2 (ja) | 2022-04-14 |
| WO2016073228A1 (en) | 2016-05-12 |
| US10308679B2 (en) | 2019-06-04 |
| KR101891455B1 (ko) | 2018-08-27 |
| JP7058560B2 (ja) | 2022-04-22 |
| SG10201801320UA (en) | 2018-04-27 |
| JP2017533701A (ja) | 2017-11-16 |
| KR20180095125A (ko) | 2018-08-24 |
| JP2018141017A (ja) | 2018-09-13 |
| CN107406514B (zh) | 2021-11-30 |
| US20190241609A1 (en) | 2019-08-08 |
| ES2742199T3 (es) | 2020-02-13 |
| SI3215614T1 (sl) | 2019-11-29 |
| US20220267750A1 (en) | 2022-08-25 |
| EP3543335A3 (en) | 2019-11-27 |
| CN107406514A (zh) | 2017-11-28 |
| CA2964119A1 (en) | 2016-05-12 |
| JP2020180162A (ja) | 2020-11-05 |
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Effective date: 20170407 |
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