JP2017533710A5 - - Google Patents
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- JP2017533710A5 JP2017533710A5 JP2017524429A JP2017524429A JP2017533710A5 JP 2017533710 A5 JP2017533710 A5 JP 2017533710A5 JP 2017524429 A JP2017524429 A JP 2017524429A JP 2017524429 A JP2017524429 A JP 2017524429A JP 2017533710 A5 JP2017533710 A5 JP 2017533710A5
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- JP
- Japan
- Prior art keywords
- capture
- primer
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- layer
- primer pair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091033319 polynucleotide Proteins 0.000 claims 36
- 102000040430 polynucleotide Human genes 0.000 claims 36
- 239000002157 polynucleotide Substances 0.000 claims 36
- 239000000758 substrate Substances 0.000 claims 25
- 239000002773 nucleotide Substances 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 20
- 238000000034 method Methods 0.000 claims 19
- 238000002493 microarray Methods 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 12
- 108020004707 nucleic acids Proteins 0.000 claims 12
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 230000000295 complement effect Effects 0.000 claims 11
- 108091093088 Amplicon Proteins 0.000 claims 10
- 239000011248 coating agent Substances 0.000 claims 9
- 238000000576 coating method Methods 0.000 claims 9
- 238000000151 deposition Methods 0.000 claims 9
- 229920000642 polymer Polymers 0.000 claims 9
- 230000003321 amplification Effects 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 3
- -1 5-azidoacetamidylpentyl Chemical group 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 1
- 108091000080 Phosphotransferase Proteins 0.000 claims 1
- 230000003213 activating effect Effects 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 230000003190 augmentative effect Effects 0.000 claims 1
- 230000007717 exclusion Effects 0.000 claims 1
- 102000020233 phosphotransferase Human genes 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021213441A JP7323598B2 (ja) | 2014-11-11 | 2021-12-27 | 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462078346P | 2014-11-11 | 2014-11-11 | |
| US62/078,346 | 2014-11-11 | ||
| US201462096464P | 2014-12-23 | 2014-12-23 | |
| US62/096,464 | 2014-12-23 | ||
| PCT/EP2015/076353 WO2016075204A1 (en) | 2014-11-11 | 2015-11-11 | Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021213441A Division JP7323598B2 (ja) | 2014-11-11 | 2021-12-27 | 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017533710A JP2017533710A (ja) | 2017-11-16 |
| JP2017533710A5 true JP2017533710A5 (enExample) | 2018-12-20 |
| JP7032930B2 JP7032930B2 (ja) | 2022-03-09 |
Family
ID=54540094
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017524429A Active JP7032930B2 (ja) | 2014-11-11 | 2015-11-11 | 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ |
| JP2021213441A Active JP7323598B2 (ja) | 2014-11-11 | 2021-12-27 | 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021213441A Active JP7323598B2 (ja) | 2014-11-11 | 2021-12-27 | 核酸のモノクローナルクラスターの生成および配列決定のための方法およびアレイ |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10619204B2 (enExample) |
| EP (1) | EP3218511B1 (enExample) |
| JP (2) | JP7032930B2 (enExample) |
| KR (1) | KR102590448B1 (enExample) |
| CN (1) | CN107208019B (enExample) |
| AU (1) | AU2015345125B2 (enExample) |
| DK (1) | DK3218511T3 (enExample) |
| ES (1) | ES2802405T3 (enExample) |
| MY (1) | MY182476A (enExample) |
| PL (1) | PL3218511T3 (enExample) |
| PT (1) | PT3218511T (enExample) |
| SG (2) | SG10202004350TA (enExample) |
| WO (1) | WO2016075204A1 (enExample) |
Families Citing this family (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110016499B (zh) | 2011-04-15 | 2023-11-14 | 约翰·霍普金斯大学 | 安全测序系统 |
| WO2014070462A1 (en) | 2012-10-29 | 2014-05-08 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| EP3027773B1 (en) | 2013-07-30 | 2020-03-25 | President and Fellows of Harvard College | Quantitative dna-based imaging and super-resolution imaging |
| US10415083B2 (en) | 2013-10-28 | 2019-09-17 | The Translational Genomics Research Institute | Long insert-based whole genome sequencing |
| KR102590448B1 (ko) | 2014-11-11 | 2023-10-17 | 일루미나 케임브리지 리미티드 | 핵산의 모노클로날 클러스터를 형성시키고 시퀀싱하기 위한 방법 및 어레이 |
| EP3283656A4 (en) * | 2015-04-17 | 2018-12-05 | Centrillion Technology Holdings Corporation | Methods for performing spatial profiling of biological molecules |
| WO2017027370A1 (en) * | 2015-08-07 | 2017-02-16 | President And Fellows Of Harvard College | Super resolution imaging of protein-protein interactions |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| JP2017209051A (ja) * | 2016-05-25 | 2017-11-30 | 株式会社ニコン | 標的生体分子の検出方法、標的生体分子検出用基板、及び標的生体分子検出装置 |
| WO2018119063A1 (en) * | 2016-12-22 | 2018-06-28 | Illumina, Inc. | Arrays with quality control tracers |
| GB201704754D0 (en) | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
| NL2019044B1 (en) * | 2017-05-11 | 2018-11-15 | Illumina Inc | Protective surface coatings for flow cells |
| US11053537B2 (en) * | 2017-05-25 | 2021-07-06 | Integrated Dna Technologies, Inc. | Reverse complement adapters for the mitigation of UMI hopping |
| US11001885B2 (en) * | 2017-06-13 | 2021-05-11 | Personal Genomics Taiwan, Inc. | Apparatus for single molecular sequencing and method of sequencing nucleic acid molecules |
| BR112020002555A2 (pt) | 2017-08-07 | 2020-08-11 | The Johns Hopkins University | métodos e materiais para avaliar e tratar câncer |
| CN107523640A (zh) * | 2017-10-12 | 2017-12-29 | 厦门燕旭安生物科技有限公司 | 一种ctDNA精准测序的扩增子文库构建方法 |
| KR102313291B1 (ko) * | 2017-12-21 | 2021-10-15 | 일루미나, 인코포레이티드 | 하이드로겔 코팅을 가진 플로우 셀 |
| JP2021525095A (ja) * | 2018-05-28 | 2021-09-24 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｄｎａ−ポア−ポリメラーゼ複合体の酵素的濃縮 |
| KR102406439B1 (ko) | 2018-06-29 | 2022-06-08 | 일루미나, 인코포레이티드 | 플로우 셀 |
| TWI730352B (zh) | 2018-08-06 | 2021-06-11 | 英商伊路米納劍橋有限公司 | 流體槽與定序系統及製備用於其之基板的方法 |
| US20200149095A1 (en) * | 2018-11-14 | 2020-05-14 | Element Biosciences, Inc. | Low binding supports for improved solid-phase dna hybridization and amplification |
| EP3891305A1 (en) * | 2018-12-05 | 2021-10-13 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
| JP7587425B2 (ja) | 2018-12-18 | 2024-11-20 | イルミナ ケンブリッジ リミテッド | 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物 |
| EP3899037B1 (en) * | 2018-12-19 | 2023-10-18 | Illumina, Inc. | Methods for improving polynucleotide cluster clonality priority |
| TWI857001B (zh) | 2019-01-29 | 2024-10-01 | 美商伊路米納有限公司 | 定序套組 |
| TWI873119B (zh) | 2019-01-29 | 2025-02-21 | 美商伊路米納有限公司 | 流通槽及製備其之方法 |
| TWI857000B (zh) | 2019-01-29 | 2024-10-01 | 美商伊路米納有限公司 | 流體槽以及將複合物引入至流體槽之方法 |
| EP3979252A4 (en) | 2019-05-31 | 2022-08-17 | NEC Corporation | INFORMATION PROCESSING DEVICE, INFORMATION PROCESSING METHOD AND INFORMATION PROCESSING PROGRAM |
| EP3976826A4 (en) * | 2019-05-31 | 2023-08-23 | Illumina, Inc. | SYSTEMS AND METHODS FOR STORAGE AND RETRIEVAL OF INFORMATION USING CELL STREAM |
| TW202120693A (zh) * | 2019-08-21 | 2021-06-01 | 美商伊路米納有限公司 | 偵測核苷酸之聚合酶併入的方法 |
| CA3135280A1 (en) * | 2019-12-16 | 2021-06-24 | Illumina, Inc. | Kits and flow cells |
| PH12021553127A1 (en) | 2019-12-20 | 2022-08-01 | Illumina Inc | Flow cells |
| US20210187470A1 (en) * | 2019-12-23 | 2021-06-24 | llumina, Inc. | Nanoparticle with single site for template polynucleotide attachment |
| EP4103748A4 (en) | 2020-02-14 | 2024-03-13 | The Johns Hopkins University | METHOD AND MATERIALS FOR ASSESSING NUCLEIC ACIDS |
| EP4107286A4 (en) * | 2020-02-19 | 2024-04-24 | Sequencing Health, Inc. | METHODS AND SYSTEMS FOR PROCESSING NUCLEIC ACIDS |
| CA3145539A1 (en) * | 2020-02-26 | 2021-09-02 | Illumina, Inc. | Kits for genotyping |
| CN115516104A (zh) * | 2020-03-03 | 2022-12-23 | 加利福尼亚太平洋生物科学股份有限公司 | 用于对双链核酸进行测序的方法和组合物 |
| KR20220151100A (ko) * | 2020-03-09 | 2022-11-14 | 일루미나, 인코포레이티드 | 핵산 서열분석의 개선 |
| CA3177286A1 (en) | 2020-05-12 | 2021-11-18 | Illumina Inc. | Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase |
| AU2021341688B2 (en) * | 2020-09-14 | 2025-09-18 | Illumina Cambridge Limited | Compositions and methods for amplifying polynucleotides |
| CA3183189A1 (en) | 2020-09-29 | 2022-04-07 | Illumina, Inc. | Flow cells and methods for making the same |
| AU2021352913A1 (en) * | 2020-09-29 | 2023-01-05 | Illumina, Inc. | Methods for making flow cells |
| US12264361B2 (en) * | 2020-12-10 | 2025-04-01 | Illumina, Inc. | Image sensor structures and related methods |
| WO2022192671A1 (en) * | 2021-03-12 | 2022-09-15 | Singular Genomics Systems, Inc. | Nanoarrays and methods of use thereof |
| US11884977B2 (en) | 2021-03-12 | 2024-01-30 | Singular Genomics Systems, Inc. | Nanoarrays and methods of use thereof |
| KR20240004418A (ko) * | 2021-04-30 | 2024-01-11 | 일루미나 케임브리지 리미티드 | 플로우 셀 및 방법 |
| WO2022240764A1 (en) * | 2021-05-10 | 2022-11-17 | Pacific Biosciences Of California, Inc. | Single-molecule seeding and amplification on a surface |
| US20250388951A1 (en) * | 2022-06-27 | 2025-12-25 | Somalogic Operating Co., Inc. | Monoclonal polony generation using nucleic acid supramolecular structures |
| EP4547387A1 (en) * | 2022-06-29 | 2025-05-07 | 10X Genomics, Inc. | Compositions and methods for oligonucleotide inversion on arrays |
| CN116024320A (zh) * | 2022-07-13 | 2023-04-28 | 上海翔琼生物技术有限公司 | 一种用于检测核酸的荧光定量pcr方法 |
| EP4584391A1 (en) * | 2022-09-09 | 2025-07-16 | TODX Ltd. | Device for parallel detection and quantification of nucleic acid based markers |
| US20240124916A1 (en) * | 2022-09-16 | 2024-04-18 | Illumina Cambridge Limited | Nanoparticle with polynucleotide binding site and method of making thereof |
| WO2024256580A1 (en) * | 2023-06-14 | 2024-12-19 | Illumina, Inc. | Concurrent sequencing with spatially separated rings |
| WO2025062001A1 (en) * | 2023-09-20 | 2025-03-27 | Illumina, Inc. | Optimised nucleic acid sequencing |
| US20250188529A1 (en) | 2023-12-12 | 2025-06-12 | Illumina, Inc. | Capturing and amplifying polynucleotides using molecules and particles |
| WO2025137479A1 (en) * | 2023-12-22 | 2025-06-26 | Illumina, Inc. | Reusable flow cells and methods of using them for nucleic acid sequencing |
| CN117778578B (zh) * | 2023-12-29 | 2024-10-15 | 深圳海普洛斯医学检验实验室 | 一种基于高通量测序检测目标基因甲基化程度的引物组合物及其应用 |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0450060A1 (en) | 1989-10-26 | 1991-10-09 | Sri International | Dna sequencing |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| ATE364718T1 (de) | 1997-04-01 | 2007-07-15 | Solexa Ltd | Verfahren zur vervielfältigung von nukleinsäure |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| CA2415897A1 (en) | 2000-07-07 | 2002-01-17 | Susan H. Hardin | Real-time sequence determination |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| GB2395954A (en) | 2002-08-23 | 2004-06-09 | Solexa Ltd | Modified nucleotides |
| AU2003272438B2 (en) | 2002-09-20 | 2009-04-02 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| EP1701785A1 (en) | 2004-01-07 | 2006-09-20 | Solexa Ltd. | Modified molecular arrays |
| EP1790202A4 (en) | 2004-09-17 | 2013-02-20 | Pacific Biosciences California | APPARATUS AND METHOD FOR ANALYZING MOLECULES |
| EP1828412B2 (en) | 2004-12-13 | 2019-01-09 | Illumina Cambridge Limited | Improved method of nucleotide detection |
| US7682816B2 (en) * | 2005-04-07 | 2010-03-23 | 454 Life Sciences Corporation | Thin film coated microwell arrays and methods of using same |
| US8623628B2 (en) | 2005-05-10 | 2014-01-07 | Illumina, Inc. | Polymerases |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| EP3722409A1 (en) | 2006-03-31 | 2020-10-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| WO2008051530A2 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| CA2672315A1 (en) | 2006-12-14 | 2008-06-26 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes using large scale fet arrays |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US20110105366A1 (en) | 2007-06-18 | 2011-05-05 | Illumina, Inc. | Microfabrication methods for the optimal patterning of substrates |
| US8728764B2 (en) | 2008-10-02 | 2014-05-20 | Illumina Cambridge Limited | Nucleic acid sample enrichment for sequencing applications |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| DK2669387T3 (en) | 2009-08-25 | 2016-09-19 | Illumina Inc | Methods of selection and amplification of polynucleotides |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| WO2012170936A2 (en) | 2011-06-09 | 2012-12-13 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
| EP3981886A1 (en) | 2011-09-23 | 2022-04-13 | Illumina, Inc. | Compositions for nucleic acid sequencing |
| EP3305400A3 (en) * | 2011-10-28 | 2018-06-06 | Illumina, Inc. | Microarray fabrication system and method |
| JP6159391B2 (ja) | 2012-04-03 | 2017-07-05 | イラミーナ インコーポレーテッド | 核酸シークエンシングに有用な統合化した読取りヘッド及び流体カートリッジ |
| US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| US9512422B2 (en) * | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| CA2928598C (en) | 2013-12-19 | 2022-11-29 | M. Shane Bowen | Substrates comprising nano-patterning surfaces and methods of preparing thereof |
| WO2015108663A1 (en) | 2014-01-16 | 2015-07-23 | Illumina, Inc. | Amplicon preparation and sequencing on solid supports |
| KR102590448B1 (ko) | 2014-11-11 | 2023-10-17 | 일루미나 케임브리지 리미티드 | 핵산의 모노클로날 클러스터를 형성시키고 시퀀싱하기 위한 방법 및 어레이 |
-
2015
- 2015-11-11 KR KR1020177015817A patent/KR102590448B1/ko active Active
- 2015-11-11 EP EP15793823.4A patent/EP3218511B1/en active Active
- 2015-11-11 JP JP2017524429A patent/JP7032930B2/ja active Active
- 2015-11-11 MY MYPI2017000708A patent/MY182476A/en unknown
- 2015-11-11 SG SG10202004350TA patent/SG10202004350TA/en unknown
- 2015-11-11 SG SG11201703693UA patent/SG11201703693UA/en unknown
- 2015-11-11 WO PCT/EP2015/076353 patent/WO2016075204A1/en not_active Ceased
- 2015-11-11 US US15/525,892 patent/US10619204B2/en active Active
- 2015-11-11 PL PL15793823T patent/PL3218511T3/pl unknown
- 2015-11-11 CN CN201580072860.9A patent/CN107208019B/zh active Active
- 2015-11-11 ES ES15793823T patent/ES2802405T3/es active Active
- 2015-11-11 DK DK15793823.4T patent/DK3218511T3/da active
- 2015-11-11 AU AU2015345125A patent/AU2015345125B2/en active Active
- 2015-11-11 PT PT157938234T patent/PT3218511T/pt unknown
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2020
- 2020-04-10 US US16/846,028 patent/US11692223B2/en active Active
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2021
- 2021-12-27 JP JP2021213441A patent/JP7323598B2/ja active Active
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2023
- 2023-05-18 US US18/320,122 patent/US12385092B2/en active Active