ES2802405T3 - Métodos y matrices para producir y secuenciar agrupaciones monoclonales de ácido nucleico - Google Patents

Métodos y matrices para producir y secuenciar agrupaciones monoclonales de ácido nucleico Download PDF

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ES2802405T3
ES2802405T3 ES15793823T ES15793823T ES2802405T3 ES 2802405 T3 ES2802405 T3 ES 2802405T3 ES 15793823 T ES15793823 T ES 15793823T ES 15793823 T ES15793823 T ES 15793823T ES 2802405 T3 ES2802405 T3 ES 2802405T3
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capture
primer
primers
pair
well
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Kevin L Gunderson
Jingwei Bai
Matthew William Kellinger
John M Beierle
Jonathan Mark Boutell
Roberto Rigatti
Bacigalupo Maria Candelaria Rogert
Boyan Boyanov
Klaus Maisinger
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Illumina Cambridge Ltd
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C40B50/14Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
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    • B01J2219/00279Features relating to reactor vessels
    • B01J2219/00306Reactor vessels in a multiple arrangement
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    • B01J2219/00306Reactor vessels in a multiple arrangement
    • B01J2219/00313Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
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    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00605Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608DNA chips
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    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00605Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632Introduction of reactive groups to the surface
    • B01J2219/00637Introduction of reactive groups to the surface by coating it with another layer
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    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
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    • B01J2219/00722Nucleotides
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    • B01L2300/0829Multi-well plates; Microtitration plates
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2565/543Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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ES15793823T 2014-11-11 2015-11-11 Métodos y matrices para producir y secuenciar agrupaciones monoclonales de ácido nucleico Active ES2802405T3 (es)

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US201462078346P 2014-11-11 2014-11-11
US201462096464P 2014-12-23 2014-12-23
PCT/EP2015/076353 WO2016075204A1 (en) 2014-11-11 2015-11-11 Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

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US (3) US10619204B2 (enExample)
EP (1) EP3218511B1 (enExample)
JP (2) JP7032930B2 (enExample)
KR (1) KR102590448B1 (enExample)
CN (1) CN107208019B (enExample)
AU (1) AU2015345125B2 (enExample)
CA (1) CA2967525A1 (enExample)
DK (1) DK3218511T3 (enExample)
ES (1) ES2802405T3 (enExample)
MY (1) MY182476A (enExample)
PL (1) PL3218511T3 (enExample)
PT (1) PT3218511T (enExample)
SG (2) SG10202004350TA (enExample)
WO (1) WO2016075204A1 (enExample)

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