BR112022026860A2 - Composições e métodos para amplificar polinucleotídeos - Google Patents

Composições e métodos para amplificar polinucleotídeos

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Publication number
BR112022026860A2
BR112022026860A2 BR112022026860A BR112022026860A BR112022026860A2 BR 112022026860 A2 BR112022026860 A2 BR 112022026860A2 BR 112022026860 A BR112022026860 A BR 112022026860A BR 112022026860 A BR112022026860 A BR 112022026860A BR 112022026860 A2 BR112022026860 A2 BR 112022026860A2
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BR
Brazil
Prior art keywords
capture primers
orthogonal
amplifiing
polynucleotides
compositions
Prior art date
Application number
BR112022026860A
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English (en)
Inventor
Fisher Jeffrey
Betley Jason
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Illumina Inc
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Illumina Inc, Illumina Cambridge Ltd filed Critical Illumina Inc
Publication of BR112022026860A2 publication Critical patent/BR112022026860A2/pt

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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

COMPOSIÇÕES E MÉTODOS PARA AMPLIFICAR POLINUCLEOTÍDEOS. A presente invenção se refere a uma composição para amplificar um polinucleotídeo que inclui um substrato que compreende uma primeira região e uma segunda região. Uma primeira pluralidade de primers de captura é acoplada à primeira região do substrato. Uma segunda pluralidade de primers de captura é acoplada à segunda região do substrato. Os primers de captura da segunda pluralidade de primers de captura são mais longos que os primers de captura da primeira pluralidade de primers de captura. Uma primeira pluralidade de primers de captura ortogonais são acoplados à primeira região do substrato. Uma segunda pluralidade de primers de captura ortogonais são acoplados à segunda região do substrato. Os primers de captura ortogonais da segunda pluralidade de primers de captura ortogonais são mais curtos que os primers de captura ortogonais da primeira pluralidade de primers de captura ortogonais.
BR112022026860A 2020-09-14 2021-08-27 Composições e métodos para amplificar polinucleotídeos BR112022026860A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063077857P 2020-09-14 2020-09-14
PCT/US2021/048064 WO2022055729A1 (en) 2020-09-14 2021-08-27 Compositions and methods for amplifying polynucleotides

Publications (1)

Publication Number Publication Date
BR112022026860A2 true BR112022026860A2 (pt) 2023-04-11

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BR112022026860A BR112022026860A2 (pt) 2020-09-14 2021-08-27 Composições e métodos para amplificar polinucleotídeos

Country Status (12)

Country Link
US (1) US11913067B2 (pt)
EP (1) EP4211269A1 (pt)
JP (1) JP2023540422A (pt)
KR (1) KR20230069047A (pt)
CN (1) CN115777024A (pt)
AU (1) AU2021341688A1 (pt)
BR (1) BR112022026860A2 (pt)
CA (1) CA3182296A1 (pt)
IL (1) IL299327A (pt)
MX (1) MX2022014724A (pt)
TW (1) TW202225412A (pt)
WO (1) WO2022055729A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3234961A1 (en) * 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
CN117987507A (zh) * 2022-11-02 2024-05-07 艾斯生物实验室有限责任公司 产生扩增子簇的方法和装置

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0514909D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods of nucleic acid amplification and sequencing
PT2669387T (pt) * 2009-08-25 2016-09-20 Illumina Inc Métodos para selecção e amplificação de polinucleótidos
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
GB201410646D0 (en) 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
ES2802405T3 (es) 2014-11-11 2021-01-19 Illumina Cambridge Ltd Métodos y matrices para producir y secuenciar agrupaciones monoclonales de ácido nucleico
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries

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Publication number Publication date
JP2023540422A (ja) 2023-09-25
MX2022014724A (es) 2023-03-06
WO2022055729A1 (en) 2022-03-17
CN115777024A (zh) 2023-03-10
IL299327A (en) 2023-02-01
AU2021341688A1 (en) 2023-01-05
TW202225412A (zh) 2022-07-01
EP4211269A1 (en) 2023-07-19
US20220090187A1 (en) 2022-03-24
KR20230069047A (ko) 2023-05-18
CA3182296A1 (en) 2022-03-17
US11913067B2 (en) 2024-02-27

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