JP2017515484A5 - - Google Patents
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- JP2017515484A5 JP2017515484A5 JP2016567863A JP2016567863A JP2017515484A5 JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5 JP 2016567863 A JP2016567863 A JP 2016567863A JP 2016567863 A JP2016567863 A JP 2016567863A JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5
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- JP
- Japan
- Prior art keywords
- subsequence
- sequence
- oligonucleotide
- blocker
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091034117 Oligonucleotide Proteins 0.000 claims 28
- 239000002773 nucleotide Substances 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 11
- 238000000034 method Methods 0.000 claims 7
- 230000000295 complement effect Effects 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 6
- 230000007935 neutral effect Effects 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 4
- 125000000524 functional group Chemical group 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 3
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 239000005546 dideoxynucleotide Substances 0.000 claims 2
- 102100034343 Integrase Human genes 0.000 claims 1
- 108060004795 Methyltransferase Proteins 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 102000018120 Recombinases Human genes 0.000 claims 1
- 108010091086 Recombinases Proteins 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 235000013367 dietary fats Nutrition 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000010520 ghee Substances 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- -1 nucleoside triphosphates Chemical class 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462000114P | 2014-05-19 | 2014-05-19 | |
| US62/000,114 | 2014-05-19 | ||
| PCT/US2015/031476 WO2015179339A1 (en) | 2014-05-19 | 2015-05-19 | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020165028A Division JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017515484A JP2017515484A (ja) | 2017-06-15 |
| JP2017515484A5 true JP2017515484A5 (enExample) | 2018-06-28 |
Family
ID=54554607
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567863A Pending JP2017515484A (ja) | 2014-05-19 | 2015-05-19 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20170067090A1 (enExample) |
| EP (3) | EP4512908A3 (enExample) |
| JP (4) | JP2017515484A (enExample) |
| KR (1) | KR102343605B1 (enExample) |
| CN (2) | CN106661627B (enExample) |
| AU (2) | AU2015264357B2 (enExample) |
| BR (1) | BR112016027063A2 (enExample) |
| CA (1) | CA2985631A1 (enExample) |
| DK (1) | DK3146080T3 (enExample) |
| ES (1) | ES2861353T3 (enExample) |
| PH (1) | PH12016502542B1 (enExample) |
| RU (1) | RU2708992C2 (enExample) |
| WO (1) | WO2015179339A1 (enExample) |
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| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
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| ES2711168T3 (es) | 2013-08-28 | 2019-04-30 | Becton Dickinson Co | Análisis masivo en paralelo de células individuales |
| WO2015077717A1 (en) | 2013-11-25 | 2015-05-28 | The Broad Institute Inc. | Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status |
| US11725237B2 (en) | 2013-12-05 | 2023-08-15 | The Broad Institute Inc. | Polymorphic gene typing and somatic change detection using sequencing data |
| EP3082853A2 (en) | 2013-12-20 | 2016-10-26 | The Broad Institute, Inc. | Combination therapy with neoantigen vaccine |
| EP3234130B1 (en) | 2014-12-19 | 2020-11-25 | The Broad Institute, Inc. | Methods for profiling the t-cell- receptor repertoire |
| US10975442B2 (en) | 2014-12-19 | 2021-04-13 | Massachusetts Institute Of Technology | Molecular biomarkers for cancer immunotherapy |
| ES2836802T3 (es) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Códigos de barras moleculares espacialmente direccionables |
| US11535882B2 (en) | 2015-03-30 | 2022-12-27 | Becton, Dickinson And Company | Methods and compositions for combinatorial barcoding |
| US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
| ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
| US10280455B2 (en) | 2015-12-30 | 2019-05-07 | Bio-Rad Laboratories, Inc. | Split-cycle and tape amplification |
| US11414686B2 (en) | 2016-05-06 | 2022-08-16 | William Marsh Rice University | Stoichiometric nucleic acid purification using randomer capture probe libraries |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
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| US11549149B2 (en) * | 2017-01-24 | 2023-01-10 | The Broad Institute, Inc. | Compositions and methods for detecting a mutant variant of a polynucleotide |
| WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| EP3635135A1 (en) | 2017-06-05 | 2020-04-15 | Becton, Dickinson and Company | Sample indexing for single cells |
| CN111566118A (zh) | 2017-10-18 | 2020-08-21 | 零日诊断有限公司 | 通过靶向抑制dna扩增来选择性富集混合dna样品中的dna群体 |
| US12331350B2 (en) * | 2018-02-20 | 2025-06-17 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| CN108220444A (zh) * | 2018-02-28 | 2018-06-29 | 无锡禾盛医疗器械有限公司 | 一种kras基因突变检测的引物探针组合及其应用 |
| WO2019213237A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
| EP4234717A3 (en) | 2018-05-03 | 2023-11-01 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| JP7670607B2 (ja) | 2018-10-01 | 2025-04-30 | ベクトン・ディキンソン・アンド・カンパニー | ５’転写物配列の決定 |
| EP3877520B1 (en) | 2018-11-08 | 2025-03-19 | Becton, Dickson And Company | Whole transcriptome analysis of single cells using random priming |
| EP3894552A1 (en) | 2018-12-13 | 2021-10-20 | Becton, Dickinson and Company | Selective extension in single cell whole transcriptome analysis |
| KR20210133215A (ko) * | 2019-01-15 | 2021-11-05 | 탄젠 바이오사이언스 인코포레이티드 | 핵산 증폭 기술에서 비특이적 증폭 산물을 억제하기 위한 방법 |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| CN120099139A (zh) | 2019-02-14 | 2025-06-06 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| CN114040983A (zh) * | 2019-06-26 | 2022-02-11 | 南京金斯瑞生物科技有限公司 | 一种含有阻断物的寡核苷酸 |
| US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| US20240279735A1 (en) * | 2019-12-20 | 2024-08-22 | Korea University Research And Business Foundation | Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same |
| CN115244184A (zh) | 2020-01-13 | 2022-10-25 | 贝克顿迪金森公司 | 用于定量蛋白和rna的方法和组合物 |
| US12188010B2 (en) | 2020-01-29 | 2025-01-07 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| EP4111168A1 (en) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| EP4172169A4 (en) * | 2020-06-26 | 2025-04-30 | William Marsh Rice University | COMPREHENSIVE AMPLICON ENRICHEMENT |
| CN111647650B (zh) * | 2020-07-06 | 2023-06-27 | 河南赛诺特生物技术有限公司 | 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| CN116547390A (zh) * | 2020-11-02 | 2023-08-04 | 阅尔基因美国公司 | 定量多重扩增子测序系统 |
| WO2022109343A1 (en) | 2020-11-20 | 2022-05-27 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| CN112522369A (zh) * | 2020-12-08 | 2021-03-19 | 合肥欧创基因生物科技有限公司 | 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法 |
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| WO2022146773A1 (en) | 2020-12-29 | 2022-07-07 | Nuprobe Usa, Inc. | Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta) |
| CN116981781A (zh) | 2021-01-15 | 2023-10-31 | 阅尔基因美国公司 | 用于高度多重定量pcr的循环多重分析 |
| WO2022197688A1 (en) | 2021-03-16 | 2022-09-22 | Nuprobe Usa, Inc. | High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection |
| WO2023077121A1 (en) * | 2021-11-01 | 2023-05-04 | Nuprobe Usa, Inc. | Rna quantitative amplicon sequencing for gene expression quantitation |
| CN119662835B (zh) * | 2025-02-20 | 2025-05-27 | 宁波熙宁检测技术有限公司 | 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法 |
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| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
| WO2014177540A1 (en) * | 2013-04-29 | 2014-11-06 | Qiagen Marseille | A method for dna amplification with a blocking oligonucleotide |
-
2015
- 2015-05-19 KR KR1020167035369A patent/KR102343605B1/ko active Active
- 2015-05-19 JP JP2016567863A patent/JP2017515484A/ja active Pending
- 2015-05-19 RU RU2016149307A patent/RU2708992C2/ru active
- 2015-05-19 CN CN201580039304.1A patent/CN106661627B/zh active Active
- 2015-05-19 WO PCT/US2015/031476 patent/WO2015179339A1/en not_active Ceased
- 2015-05-19 CN CN202111356156.XA patent/CN114032234A/zh active Pending
- 2015-05-19 CA CA2985631A patent/CA2985631A1/en active Pending
- 2015-05-19 EP EP24208177.6A patent/EP4512908A3/en active Pending
- 2015-05-19 BR BR112016027063A patent/BR112016027063A2/pt not_active Application Discontinuation
- 2015-05-19 EP EP21150302.4A patent/EP3901278B1/en active Active
- 2015-05-19 ES ES15796383T patent/ES2861353T3/es active Active
- 2015-05-19 AU AU2015264357A patent/AU2015264357B2/en active Active
- 2015-05-19 DK DK15796383.6T patent/DK3146080T3/da active
- 2015-05-19 EP EP15796383.6A patent/EP3146080B1/en active Active
- 2015-05-19 PH PH1/2016/502542A patent/PH12016502542B1/en unknown
-
2016
- 2016-11-18 US US15/355,235 patent/US20170067090A1/en not_active Abandoned
-
2020
- 2020-09-30 JP JP2020165028A patent/JP7126718B2/ja active Active
-
2021
- 2021-11-11 AU AU2021266291A patent/AU2021266291C1/en active Active
- 2021-11-29 US US17/536,204 patent/US20220090168A1/en active Pending
-
2022
- 2022-08-08 JP JP2022126459A patent/JP2022166131A/ja active Pending
-
2024
- 2024-09-06 JP JP2024153764A patent/JP2024167406A/ja active Pending
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