JP2017515484A5 - - Google Patents
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- JP2017515484A5 JP2017515484A5 JP2016567863A JP2016567863A JP2017515484A5 JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5 JP 2016567863 A JP2016567863 A JP 2016567863A JP 2016567863 A JP2016567863 A JP 2016567863A JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5
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- JP
- Japan
- Prior art keywords
- subsequence
- sequence
- oligonucleotide
- blocker
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462000114P | 2014-05-19 | 2014-05-19 | |
| US62/000,114 | 2014-05-19 | ||
| PCT/US2015/031476 WO2015179339A1 (en) | 2014-05-19 | 2015-05-19 | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020165028A Division JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017515484A JP2017515484A (ja) | 2017-06-15 |
| JP2017515484A5 true JP2017515484A5 (enExample) | 2018-06-28 |
Family
ID=54554607
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567863A Pending JP2017515484A (ja) | 2014-05-19 | 2015-05-19 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20170067090A1 (enExample) |
| EP (3) | EP3901278B1 (enExample) |
| JP (4) | JP2017515484A (enExample) |
| KR (1) | KR102343605B1 (enExample) |
| CN (2) | CN106661627B (enExample) |
| AU (2) | AU2015264357B2 (enExample) |
| BR (1) | BR112016027063A2 (enExample) |
| CA (1) | CA2985631A1 (enExample) |
| DK (1) | DK3146080T3 (enExample) |
| ES (1) | ES2861353T3 (enExample) |
| PH (1) | PH12016502542B1 (enExample) |
| RU (1) | RU2708992C2 (enExample) |
| WO (1) | WO2015179339A1 (enExample) |
Families Citing this family (56)
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| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| US9567645B2 (en) | 2013-08-28 | 2017-02-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| US10801070B2 (en) | 2013-11-25 | 2020-10-13 | The Broad Institute, Inc. | Compositions and methods for diagnosing, evaluating and treating cancer |
| US11725237B2 (en) | 2013-12-05 | 2023-08-15 | The Broad Institute Inc. | Polymorphic gene typing and somatic change detection using sequencing data |
| NZ721908A (en) | 2013-12-20 | 2022-12-23 | Massachusetts Gen Hospital | Combination therapy with neoantigen vaccine |
| EP4636401A2 (en) | 2014-12-19 | 2025-10-22 | The Broad Institute, Inc. | Methods for profiling the t-cell-receptor repertoire |
| US10975442B2 (en) | 2014-12-19 | 2021-04-13 | Massachusetts Institute Of Technology | Molecular biomarkers for cancer immunotherapy |
| CN107208158B (zh) | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
| EP3835431B1 (en) | 2015-03-30 | 2022-11-02 | Becton, Dickinson and Company | Methods for combinatorial barcoding |
| US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| EP3397765B1 (en) * | 2015-12-30 | 2023-02-01 | Bio-Rad Laboratories, Inc. | Method for quantitating the frequency of wild-type and mutant fragments in a nucleic acid sample |
| CN109563506A (zh) | 2016-05-06 | 2019-04-02 | 威廉马歇莱思大学 | 使用随机捕获探针文库的化学计量核酸纯化 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| AU2017331459B2 (en) | 2016-09-26 | 2023-04-13 | Becton, Dickinson And Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| US11549149B2 (en) * | 2017-01-24 | 2023-01-10 | The Broad Institute, Inc. | Compositions and methods for detecting a mutant variant of a polynucleotide |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| WO2018226293A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| EP3697798A4 (en) * | 2017-10-18 | 2021-08-11 | Day Zero Diagnostics, Inc. | SELECTIVE ENRICHMENT OF A POPULATION OF DNA IN A MIXED DNA SAMPLE BY TARGETED DELETION OF DNA AMPLIFICATION |
| US12331350B2 (en) * | 2018-02-20 | 2025-06-17 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| CN108220444A (zh) * | 2018-02-28 | 2018-06-29 | 无锡禾盛医疗器械有限公司 | 一种kras基因突变检测的引物探针组合及其应用 |
| EP3788170B1 (en) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| WO2020072380A1 (en) | 2018-10-01 | 2020-04-09 | Cellular Research, Inc. | Determining 5' transcript sequences |
| EP3877520B1 (en) | 2018-11-08 | 2025-03-19 | Becton, Dickson And Company | Whole transcriptome analysis of single cells using random priming |
| EP4745247A2 (en) | 2018-12-13 | 2026-05-20 | Becton Dickinson Co | SELECTIVE EXTENSION IN A SINGLE-CELL WHOLE-TRANSCRIPTOME ANALYSIS |
| EP3911754A4 (en) * | 2019-01-15 | 2022-10-12 | Tangen Biosciences Inc. | METHOD FOR REMOVAL OF NON-SPECIFIC AMPLIFICATION PRODUCTS IN NUCLEIC ACID AMPLIFICATION TECHNOLOGIES |
| CN113574178B (zh) | 2019-01-23 | 2024-10-29 | 贝克顿迪金森公司 | 与抗体关联的寡核苷酸 |
| CN120099139A (zh) | 2019-02-14 | 2025-06-06 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US20220348606A1 (en) * | 2019-06-26 | 2022-11-03 | Nanjing GenScript Biotech Co., Ltd. | Oligonucleotide containing blocker |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| EP4055160B1 (en) | 2019-11-08 | 2024-04-10 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
| US20240279735A1 (en) * | 2019-12-20 | 2024-08-22 | Korea University Research And Business Foundation | Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same |
| EP4090763B1 (en) | 2020-01-13 | 2024-12-04 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| WO2021222798A1 (en) * | 2020-05-01 | 2021-11-04 | William Marsh Rice University | Quantitative blocker displacement amplification (qbda) sequencing for calibration-free and multiplexed variant allele frequency quantitation |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| AU2021294360A1 (en) * | 2020-06-26 | 2023-02-02 | William Marsh Rice University | Amplicon Comprehensive Enrichment |
| CN111647650B (zh) * | 2020-07-06 | 2023-06-27 | 河南赛诺特生物技术有限公司 | 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| CN116547390A (zh) * | 2020-11-02 | 2023-08-04 | 阅尔基因美国公司 | 定量多重扩增子测序系统 |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| CN112522369A (zh) * | 2020-12-08 | 2021-03-19 | 合肥欧创基因生物科技有限公司 | 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法 |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| WO2022146773A1 (en) | 2020-12-29 | 2022-07-07 | Nuprobe Usa, Inc. | Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta) |
| WO2022155397A1 (en) | 2021-01-15 | 2022-07-21 | Nuprobe Usa, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
| WO2022197688A1 (en) | 2021-03-16 | 2022-09-22 | Nuprobe Usa, Inc. | High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection |
| WO2023077121A1 (en) * | 2021-11-01 | 2023-05-04 | Nuprobe Usa, Inc. | Rna quantitative amplicon sequencing for gene expression quantitation |
| CN120624641A (zh) * | 2025-02-20 | 2025-09-12 | 宁波熙宁检测技术有限公司 | 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6312892B1 (en) * | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| KR20070090158A (ko) * | 2004-10-18 | 2007-09-05 | 브랜데이스 유니버시티 | Ｐｃｒ 증폭에서 재현성을 개선시키고 미스프라이밍을감소시키기 위한 시약 및 방법 |
| US20100009355A1 (en) * | 2006-03-14 | 2010-01-14 | Kolodney Michael S | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
| US8071338B2 (en) * | 2007-08-08 | 2011-12-06 | Roche Molecular Systems, Inc. | Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity |
| JP5535921B2 (ja) * | 2007-10-04 | 2014-07-02 | コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション | 核酸増幅 |
| WO2010046067A1 (en) * | 2008-10-20 | 2010-04-29 | Roche Diagnostics Gmbh | Improved allele-specific amplification |
| US8206929B2 (en) * | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| EP3249053A1 (en) * | 2009-03-27 | 2017-11-29 | Life Technologies Corporation | Methods, compositions, and kits for detecting allelic variants |
| KR101312241B1 (ko) * | 2010-04-27 | 2013-09-27 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
| WO2011146403A2 (en) * | 2010-05-16 | 2011-11-24 | Reniguard Life Sciences, Inc. | Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use |
| CA2824223A1 (en) * | 2011-01-14 | 2012-07-19 | Genefirst Limited | Methods, compositions, and kits for determining the presence/absence of a variant nucleic acid sequence |
| EP2705162B1 (en) * | 2011-05-04 | 2018-07-11 | Biocept, Inc. | Methods for detecting nucleic acid sequence variants |
| US9758818B2 (en) * | 2011-12-30 | 2017-09-12 | Jr-Kai Huang | Polymerase chain reaction-based method and primer set for detecting epidermal growth factor receptor mutation |
| EP2841598B1 (en) * | 2012-04-25 | 2018-03-14 | Ho, Tho Huu | Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products |
| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
| JP6652693B2 (ja) * | 2013-04-29 | 2020-02-26 | キアゲン ゲーエムベーハー | ブロッキングオリゴヌクレオチドを用いたｄｎａ増幅の方法 |
-
2015
- 2015-05-19 KR KR1020167035369A patent/KR102343605B1/ko active Active
- 2015-05-19 DK DK15796383.6T patent/DK3146080T3/da active
- 2015-05-19 CN CN201580039304.1A patent/CN106661627B/zh active Active
- 2015-05-19 CA CA2985631A patent/CA2985631A1/en active Pending
- 2015-05-19 CN CN202111356156.XA patent/CN114032234A/zh active Pending
- 2015-05-19 ES ES15796383T patent/ES2861353T3/es active Active
- 2015-05-19 AU AU2015264357A patent/AU2015264357B2/en active Active
- 2015-05-19 BR BR112016027063A patent/BR112016027063A2/pt not_active Application Discontinuation
- 2015-05-19 EP EP21150302.4A patent/EP3901278B1/en active Active
- 2015-05-19 EP EP15796383.6A patent/EP3146080B1/en active Active
- 2015-05-19 RU RU2016149307A patent/RU2708992C2/ru active
- 2015-05-19 JP JP2016567863A patent/JP2017515484A/ja active Pending
- 2015-05-19 EP EP24208177.6A patent/EP4512908A3/en active Pending
- 2015-05-19 PH PH1/2016/502542A patent/PH12016502542B1/en unknown
- 2015-05-19 WO PCT/US2015/031476 patent/WO2015179339A1/en not_active Ceased
-
2016
- 2016-11-18 US US15/355,235 patent/US20170067090A1/en not_active Abandoned
-
2020
- 2020-09-30 JP JP2020165028A patent/JP7126718B2/ja active Active
-
2021
- 2021-11-11 AU AU2021266291A patent/AU2021266291C1/en active Active
- 2021-11-29 US US17/536,204 patent/US20220090168A1/en active Pending
-
2022
- 2022-08-08 JP JP2022126459A patent/JP2022166131A/ja active Pending
-
2024
- 2024-09-06 JP JP2024153764A patent/JP2024167406A/ja active Pending
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