BR112016027063A2 - amplificação de alelo específico que usa uma composição de sobreposição de iniciador não alelo específico e oligonucleotídeos bloqueadores de alelo específico - Google Patents

amplificação de alelo específico que usa uma composição de sobreposição de iniciador não alelo específico e oligonucleotídeos bloqueadores de alelo específico

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Publication number
BR112016027063A2
BR112016027063A2 BR112016027063A BR112016027063A BR112016027063A2 BR 112016027063 A2 BR112016027063 A2 BR 112016027063A2 BR 112016027063 A BR112016027063 A BR 112016027063A BR 112016027063 A BR112016027063 A BR 112016027063A BR 112016027063 A2 BR112016027063 A2 BR 112016027063A2
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Brazil
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allele
specific
subsequence
sequence
oligonucleotide
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BR112016027063A
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English (en)
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Yu Zhang David
Wang Juexiao
Wu Ruojia
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Univ Rice William M
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Application filed by Univ Rice William M filed Critical Univ Rice William M
Publication of BR112016027063A2 publication Critical patent/BR112016027063A2/pt

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

resumo "amplificação de alelo específico que usa uma composição de sobreposição de iniciador não alelo específico e oligonucleotídeos bloqueadores de alelo específico" a presente invenção fornece uma composição incluindo um oligonucleotídeo bloqueador e um primeiro oligonucleotídeo iniciador. o oligonucleotídeo bloqueador inclui uma primeira sequência tendo uma subsequência alvo neutro e uma subsequência variável bloqueador. a subsequência específica não alvo é flanqueada na sua extremidade 3 'e 5' pela subsequência alvo neutra e é contínua com a subsequência alvo neutro. o primeiro oligonucleotídeo iniciador é suficiente para induzir a extensão enzimática; aqui o primeiro oligonucleotídeo iniciador inclui uma segunda sequência. a segunda sequência se sobrepõe com a extremidade 5 'da subsequência alvo neutra por pelo menos 5 nucleotídeos; aqui a segunda sequênncia não inclui a subsequência específica não alvo.
BR112016027063A 2014-05-19 2015-05-19 amplificação de alelo específico que usa uma composição de sobreposição de iniciador não alelo específico e oligonucleotídeos bloqueadores de alelo específico BR112016027063A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462000114P 2014-05-19 2014-05-19
PCT/US2015/031476 WO2015179339A1 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Publications (1)

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BR112016027063A2 true BR112016027063A2 (pt) 2017-10-31

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US (2) US20170067090A1 (pt)
EP (2) EP3146080B1 (pt)
JP (3) JP2017515484A (pt)
KR (1) KR102343605B1 (pt)
CN (2) CN106661627B (pt)
AU (2) AU2015264357B2 (pt)
BR (1) BR112016027063A2 (pt)
CA (1) CA2985631A1 (pt)
DK (1) DK3146080T3 (pt)
ES (1) ES2861353T3 (pt)
PH (1) PH12016502542A1 (pt)
RU (1) RU2708992C2 (pt)
WO (1) WO2015179339A1 (pt)

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EP3146080B1 (en) 2021-01-06
KR20170003695A (ko) 2017-01-09
US20220090168A1 (en) 2022-03-24
RU2708992C2 (ru) 2019-12-12
CN106661627B (zh) 2021-11-23
US20170067090A1 (en) 2017-03-09
PH12016502542A1 (en) 2017-04-10
JP7126718B2 (ja) 2022-08-29
JP2022166131A (ja) 2022-11-01
EP3146080A4 (en) 2017-11-01
AU2015264357B2 (en) 2021-08-12
CN106661627A (zh) 2017-05-10
EP3901278A1 (en) 2021-10-27
JP2021019607A (ja) 2021-02-18
JP2017515484A (ja) 2017-06-15
AU2015264357A1 (en) 2017-01-05
KR102343605B1 (ko) 2021-12-27
DK3146080T3 (en) 2021-04-12
AU2021266291A1 (en) 2021-12-09
RU2016149307A3 (pt) 2018-12-10
CN114032234A (zh) 2022-02-11
ES2861353T3 (es) 2021-10-06
WO2015179339A1 (en) 2015-11-26
CA2985631A1 (en) 2015-11-26
RU2016149307A (ru) 2018-06-20
EP3146080A1 (en) 2017-03-29

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