CN114040983A - 一种含有阻断物的寡核苷酸 - Google Patents

一种含有阻断物的寡核苷酸 Download PDF

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CN114040983A
CN114040983A CN202080046642.9A CN202080046642A CN114040983A CN 114040983 A CN114040983 A CN 114040983A CN 202080046642 A CN202080046642 A CN 202080046642A CN 114040983 A CN114040983 A CN 114040983A
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oligonucleotide
carbon
seq
bonds
combination
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王建鹏
赵禹
吴政宪
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Nanjing Jinsirui Science and Technology Biology Corp
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Nanjing Jinsirui Science and Technology Biology Corp
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7125—Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

涉及目标序列杂交捕获以及通过阻断寡核苷酸设计与合成领域，具体涉及一种含有阻断物的寡核苷酸，应用该具有阻断作用的寡核苷酸或其组合，不仅在单个样本的目标序列捕获时，对接头序列具有良好的阻断的效果，降低非特异性捕获，提高捕获效率，特别是能够实现对多个样本中目标序列两端的接头序列的有效阻断，提高目标序列的捕获效率。

Description

PCT国内申请，说明书已公开。

Claims (34)

  1. PCT国内申请，权利要求书已公开。
CN202080046642.9A 2019-06-26 2020-06-28 一种含有阻断物的寡核苷酸 Pending CN114040983A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2019105608410 2019-06-26
CN201910560841 2019-06-26
PCT/CN2020/098473 WO2020259678A1 (zh) 2019-06-26 2020-06-28 一种含有阻断物的寡核苷酸

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CN114040983A true CN114040983A (zh) 2022-02-11

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CN202080046642.9A Pending CN114040983A (zh) 2019-06-26 2020-06-28 一种含有阻断物的寡核苷酸

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US (1) US20220348606A1 (zh)
CN (1) CN114040983A (zh)
WO (1) WO2020259678A1 (zh)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658738A (en) * 1994-05-31 1997-08-19 Becton Dickinson And Company Bi-directional oligonucleotides that bind thrombin
CN1452664A (zh) * 2000-07-07 2003-10-29 海伦·李 检棒分析中改良的结合相互作用
CN105209634A (zh) * 2013-03-08 2015-12-30 牛津纳米孔技术公司 酶停滞方法
CN108676846A (zh) * 2018-05-25 2018-10-19 艾吉泰康生物科技(北京)有限公司 一种桥式寡核苷酸在文库目标区域捕获中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015073163A2 (en) * 2013-11-14 2015-05-21 Trovagene, Inc. Synthesis and enrichment of nucleic acid sequences
RU2708992C2 (ru) * 2014-05-19 2019-12-12 Уильям Марш Райс Юниверсити Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора
JP6722179B2 (ja) * 2014-10-10 2020-07-15 インヴァイティ コーポレイションＩｎｖｉｔａｅ Ｃｏｒｐｏｒａｔｉｏｎ 多重キャプチャー反応のためのユニバーサルブロッキングオリゴシステム及び改良されたハイブリダイゼーションキャプチャー方法
CN106987585B (zh) * 2017-03-15 2019-11-12 深圳市海普洛斯生物科技有限公司 一种针对cfDNA的单链DNA二代测序文库构建方法
WO2019071474A1 (zh) * 2017-10-11 2019-04-18 深圳华大智造科技有限公司 修饰的核苷或核苷酸

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658738A (en) * 1994-05-31 1997-08-19 Becton Dickinson And Company Bi-directional oligonucleotides that bind thrombin
CN1452664A (zh) * 2000-07-07 2003-10-29 海伦·李 检棒分析中改良的结合相互作用
CN105209634A (zh) * 2013-03-08 2015-12-30 牛津纳米孔技术公司 酶停滞方法
CN108676846A (zh) * 2018-05-25 2018-10-19 艾吉泰康生物科技(北京)有限公司 一种桥式寡核苷酸在文库目标区域捕获中的应用

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WO2020259678A1 (zh) 2020-12-30
US20220348606A1 (en) 2022-11-03

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