CN104185683B - 用以检测突变的方法与引物组 - Google Patents
用以检测突变的方法与引物组 Download PDFInfo
- Publication number
- CN104185683B CN104185683B CN201180076166.6A CN201180076166A CN104185683B CN 104185683 B CN104185683 B CN 104185683B CN 201180076166 A CN201180076166 A CN 201180076166A CN 104185683 B CN104185683 B CN 104185683B
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- Prior art keywords
- primer
- sequence
- suppression
- selection area
- nucleotide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (19)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2011/085015 WO2013097173A1 (en) | 2011-12-30 | 2011-12-30 | Method and primer set for detecting mutation |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104185683A CN104185683A (zh) | 2014-12-03 |
CN104185683B true CN104185683B (zh) | 2016-06-01 |
Family
ID=48696246
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201180076166.6A Active CN104185683B (zh) | 2011-12-30 | 2011-12-30 | 用以检测突变的方法与引物组 |
Country Status (5)
Country | Link |
---|---|
US (1) | US9758818B2 (zh) |
EP (1) | EP2798078B1 (zh) |
CN (1) | CN104185683B (zh) |
AU (1) | AU2011384896B2 (zh) |
WO (1) | WO2013097173A1 (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014005278A1 (en) * | 2012-07-03 | 2014-01-09 | Jr-Kai Huang | Self-competitive primer and method of use |
KR102343605B1 (ko) * | 2014-05-19 | 2021-12-27 | 윌리엄 마쉬 라이스 유니버시티 | 비대립 유전자-특이적 프라이머 및 대립 유전자-특이적 블로커 올리고뉴클레오티드를 중첩하는 조성물을 사용한 대립 유전자-특이적 증폭 |
US20170211139A1 (en) * | 2014-07-11 | 2017-07-27 | Exosome Diagnostics, Inc. | Compositions and methods for detecting rare sequence variants in nucleic acid sequencing |
WO2017193025A1 (en) | 2016-05-06 | 2017-11-09 | William Marsh Rice University | Stoichiometric nucleic acid purification using randomer capture probe libraries |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2293238A (en) * | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
US20040121364A1 (en) * | 2000-02-07 | 2004-06-24 | Mark Chee | Multiplex nucleic acid reactions |
CN1670222A (zh) * | 2004-03-18 | 2005-09-21 | 华晶基因技术有限公司 | 单核苷酸多态性(SNPs)及点突变的检测方法 |
CN1769489A (zh) * | 2005-10-20 | 2006-05-10 | 湖北迪亚生物工程有限责任公司 | Pcr阻断联合荧光探针检测基因突变的方法及其所用的引物 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007008997A2 (en) * | 2005-07-12 | 2007-01-18 | The Board Of Trustees Of The Leland Stanford Junior University | Template specific inhibition of pcr |
KR101312241B1 (ko) * | 2010-04-27 | 2013-09-27 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
WO2011136462A1 (ko) * | 2010-04-27 | 2011-11-03 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
-
2011
- 2011-12-30 EP EP11878641.7A patent/EP2798078B1/en active Active
- 2011-12-30 WO PCT/CN2011/085015 patent/WO2013097173A1/en active Application Filing
- 2011-12-30 CN CN201180076166.6A patent/CN104185683B/zh active Active
- 2011-12-30 AU AU2011384896A patent/AU2011384896B2/en not_active Ceased
- 2011-12-30 US US14/370,000 patent/US9758818B2/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2293238A (en) * | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
US20040121364A1 (en) * | 2000-02-07 | 2004-06-24 | Mark Chee | Multiplex nucleic acid reactions |
CN1670222A (zh) * | 2004-03-18 | 2005-09-21 | 华晶基因技术有限公司 | 单核苷酸多态性(SNPs)及点突变的检测方法 |
CN1769489A (zh) * | 2005-10-20 | 2006-05-10 | 湖北迪亚生物工程有限责任公司 | Pcr阻断联合荧光探针检测基因突变的方法及其所用的引物 |
Non-Patent Citations (4)
Title |
---|
《ATP13A2 variability in Parkinson disease》;Carles V-G;《Hum Mutat》;20090331;第30卷(第3期);406-410 * |
《Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers》;Shigematsu H;《Natl Cancer Inst》;20050302;第97卷(第5期);339-346 * |
《帕金森病与ATP13A2基因》;梁卉;《基础医学与临床》;20111031;第31卷(第10期);1161-1164 * |
《肺腺癌组织中EGFR-19和21外显子突变状况分析》;魏冰;《河南医学研究》;20101231;第19卷(第4期);407-410 * |
Also Published As
Publication number | Publication date |
---|---|
US9758818B2 (en) | 2017-09-12 |
EP2798078B1 (en) | 2018-02-14 |
US20150037795A1 (en) | 2015-02-05 |
CN104185683A (zh) | 2014-12-03 |
AU2011384896A1 (en) | 2014-07-24 |
EP2798078A1 (en) | 2014-11-05 |
WO2013097173A1 (en) | 2013-07-04 |
AU2011384896B2 (en) | 2016-02-18 |
EP2798078A4 (en) | 2015-10-07 |
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Effective date of registration: 20170824 Address after: Chinese Taiwan New Taipei City Patentee after: King of Kings biological Polytron Technologies Inc. Address before: Banqiao District, New Taipei City Taiwan road Chinese Hanson 270 No. 3 18 floor Co-patentee before: Chen Jikuan Patentee before: Huang Zhikai Co-patentee before: Wang Daoyuan |
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Effective date of registration: 20181211 Address after: Chinese Taiwan New Taipei City Patentee after: Hongjin Biotechnology Co.,Ltd. Address before: Chinese Taiwan New Taipei City Patentee before: King of Kings biological Polytron Technologies Inc. |
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Effective date of registration: 20230425 Address after: Chinese Taiwan New Taipei City Patentee after: AccuBioMed Co.,Ltd. Address before: Chinese Taiwan New Taipei City Patentee before: Hongjin Biotechnology Co.,Ltd. |