JP2024167406A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2024167406A5 JP2024167406A5 JP2024153764A JP2024153764A JP2024167406A5 JP 2024167406 A5 JP2024167406 A5 JP 2024167406A5 JP 2024153764 A JP2024153764 A JP 2024153764A JP 2024153764 A JP2024153764 A JP 2024153764A JP 2024167406 A5 JP2024167406 A5 JP 2024167406A5
- Authority
- JP
- Japan
- Prior art keywords
- subsequence
- sequence
- blocker
- target
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims 47
- 108020004707 nucleic acids Proteins 0.000 claims 25
- 150000007523 nucleic acids Chemical class 0.000 claims 25
- 102000039446 nucleic acids Human genes 0.000 claims 25
- 230000007935 neutral effect Effects 0.000 claims 23
- 230000000295 complement effect Effects 0.000 claims 16
- 239000002773 nucleotide Substances 0.000 claims 15
- 125000003729 nucleotide group Chemical group 0.000 claims 15
- 238000009396 hybridization Methods 0.000 claims 9
- 238000000034 method Methods 0.000 claims 8
- 230000003321 amplification Effects 0.000 claims 6
- 230000002255 enzymatic effect Effects 0.000 claims 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 238000012217 deletion Methods 0.000 claims 2
- 230000037430 deletion Effects 0.000 claims 2
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 239000002777 nucleoside Substances 0.000 claims 2
- -1 nucleoside triphosphates Chemical class 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462000114P | 2014-05-19 | 2014-05-19 | |
| US62/000,114 | 2014-05-19 | ||
| JP2020165028A JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022126459A Division JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2024167406A JP2024167406A (ja) | 2024-12-03 |
| JP2024167406A5 true JP2024167406A5 (enExample) | 2025-02-04 |
Family
ID=54554607
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567863A Pending JP2017515484A (ja) | 2014-05-19 | 2015-05-19 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2024153764A Pending JP2024167406A (ja) | 2014-05-19 | 2024-09-06 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Family Applications Before (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567863A Pending JP2017515484A (ja) | 2014-05-19 | 2015-05-19 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2020165028A Active JP7126718B2 (ja) | 2014-05-19 | 2020-09-30 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
| JP2022126459A Pending JP2022166131A (ja) | 2014-05-19 | 2022-08-08 | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20170067090A1 (enExample) |
| EP (3) | EP4512908A3 (enExample) |
| JP (4) | JP2017515484A (enExample) |
| KR (1) | KR102343605B1 (enExample) |
| CN (2) | CN114032234A (enExample) |
| AU (2) | AU2015264357B2 (enExample) |
| BR (1) | BR112016027063A2 (enExample) |
| CA (1) | CA2985631A1 (enExample) |
| DK (1) | DK3146080T3 (enExample) |
| ES (1) | ES2861353T3 (enExample) |
| PH (1) | PH12016502542B1 (enExample) |
| RU (1) | RU2708992C2 (enExample) |
| WO (1) | WO2015179339A1 (enExample) |
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| WO2015077717A1 (en) | 2013-11-25 | 2015-05-28 | The Broad Institute Inc. | Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status |
| US11725237B2 (en) | 2013-12-05 | 2023-08-15 | The Broad Institute Inc. | Polymorphic gene typing and somatic change detection using sequencing data |
| EP3082853A2 (en) | 2013-12-20 | 2016-10-26 | The Broad Institute, Inc. | Combination therapy with neoantigen vaccine |
| EP3234193B1 (en) | 2014-12-19 | 2020-07-15 | Massachusetts Institute of Technology | Molecular biomarkers for cancer immunotherapy |
| US10993997B2 (en) | 2014-12-19 | 2021-05-04 | The Broad Institute, Inc. | Methods for profiling the t cell repertoire |
| EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP3286326B1 (en) | 2015-04-23 | 2025-01-22 | Becton, Dickinson and Company | Method for whole transcriptome amplification |
| EP3347465B1 (en) | 2015-09-11 | 2019-06-26 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
| EP3397765B1 (en) * | 2015-12-30 | 2023-02-01 | Bio-Rad Laboratories, Inc. | Method for quantitating the frequency of wild-type and mutant fragments in a nucleic acid sample |
| US11414686B2 (en) | 2016-05-06 | 2022-08-16 | William Marsh Rice University | Stoichiometric nucleic acid purification using randomer capture probe libraries |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| US11549149B2 (en) * | 2017-01-24 | 2023-01-10 | The Broad Institute, Inc. | Compositions and methods for detecting a mutant variant of a polynucleotide |
| WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| AU2018281745B2 (en) | 2017-06-05 | 2022-05-19 | Becton, Dickinson And Company | Sample indexing for single cells |
| JP2021500077A (ja) * | 2017-10-18 | 2021-01-07 | デイ ゼロ ダイアグノスティックス， インコーポレイテッド | Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化 |
| EP3759118A4 (en) * | 2018-02-20 | 2022-03-23 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| CN108220444A (zh) * | 2018-02-28 | 2018-06-29 | 无锡禾盛医疗器械有限公司 | 一种kras基因突变检测的引物探针组合及其应用 |
| ES2945191T3 (es) | 2018-05-03 | 2023-06-29 | Becton Dickinson Co | Análisis de muestras multiómicas de alto rendimiento |
| EP3788170B1 (en) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| WO2020097315A1 (en) | 2018-11-08 | 2020-05-14 | Cellular Research, Inc. | Whole transcriptome analysis of single cells using random priming |
| CN113195717A (zh) * | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| CA3122844A1 (en) * | 2019-01-15 | 2020-07-23 | Tangen Bioscience Inc. | A method for suppressing non-specific amplification products in nucleic acid amplification technologies |
| WO2020154247A1 (en) | 2019-01-23 | 2020-07-30 | Cellular Research, Inc. | Oligonucleotides associated with antibodies |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| CN114040983A (zh) * | 2019-06-26 | 2022-02-11 | 南京金斯瑞生物科技有限公司 | 一种含有阻断物的寡核苷酸 |
| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| EP4407041A3 (en) | 2019-11-08 | 2024-09-25 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
| US20240279735A1 (en) * | 2019-12-20 | 2024-08-22 | Korea University Research And Business Foundation | Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| EP4471155A3 (en) | 2020-01-29 | 2024-12-18 | Becton, Dickinson and Company | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| KR20230028450A (ko) * | 2020-06-26 | 2023-02-28 | 윌리엄 마쉬 라이스 유니버시티 | 앰플리콘 포괄적 풍부화 |
| CN111647650B (zh) * | 2020-07-06 | 2023-06-27 | 河南赛诺特生物技术有限公司 | 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US20230399687A1 (en) * | 2020-11-02 | 2023-12-14 | Nuprobe Usa, Inc. | Quantitative Multiplex Amplicon Sequencing System |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| CN112522369A (zh) * | 2020-12-08 | 2021-03-19 | 合肥欧创基因生物科技有限公司 | 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法 |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| WO2022146773A1 (en) | 2020-12-29 | 2022-07-07 | Nuprobe Usa, Inc. | Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta) |
| WO2022155397A1 (en) | 2021-01-15 | 2022-07-21 | Nuprobe Usa, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
| WO2022197688A1 (en) | 2021-03-16 | 2022-09-22 | Nuprobe Usa, Inc. | High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection |
| WO2023077121A1 (en) * | 2021-11-01 | 2023-05-04 | Nuprobe Usa, Inc. | Rna quantitative amplicon sequencing for gene expression quantitation |
| CN120624641A (zh) * | 2025-02-20 | 2025-09-12 | 宁波熙宁检测技术有限公司 | 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6312892B1 (en) * | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| NZ554701A (en) * | 2004-10-18 | 2010-03-26 | Univ Brandeis | Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification |
| WO2007106534A2 (en) * | 2006-03-14 | 2007-09-20 | Harbor-Ucla Research And Education Institute | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
| US8071338B2 (en) * | 2007-08-08 | 2011-12-06 | Roche Molecular Systems, Inc. | Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity |
| WO2009043112A1 (en) * | 2007-10-04 | 2009-04-09 | Commonwealth Scientific And Industrial Research Organisation | Nucleic acid amplification |
| EP2337865B1 (en) * | 2008-10-20 | 2014-11-19 | Roche Diagnostics GmbH | Allele-specific amplification using a primer with a modified nucleotide |
| US8206929B2 (en) * | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| CN102428190B (zh) * | 2009-03-27 | 2014-02-26 | 生命技术公司 | 用于检测等位基因变体的方法、组合物和试剂盒 |
| KR101312241B1 (ko) * | 2010-04-27 | 2013-09-27 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
| WO2011146403A2 (en) * | 2010-05-16 | 2011-11-24 | Reniguard Life Sciences, Inc. | Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use |
| GB2487632B (en) * | 2011-01-14 | 2014-03-12 | Genefirst Ltd | Methods,compositions,and kits for determining the presence/absence of a variant nucleic acid sequence |
| KR20210056458A (ko) * | 2011-05-04 | 2021-05-18 | 바이오셉트 인코포레이티드 | 핵산 서열 변이체를 검출하는 방법 |
| US9758818B2 (en) * | 2011-12-30 | 2017-09-12 | Jr-Kai Huang | Polymerase chain reaction-based method and primer set for detecting epidermal growth factor receptor mutation |
| EP2841598B1 (en) * | 2012-04-25 | 2018-03-14 | Ho, Tho Huu | Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products |
| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
| US10760118B2 (en) * | 2013-04-29 | 2020-09-01 | Qiagen Gmbh | Method for DNA amplification with a blocking oligonucleotide |
-
2015
- 2015-05-19 PH PH1/2016/502542A patent/PH12016502542B1/en unknown
- 2015-05-19 DK DK15796383.6T patent/DK3146080T3/da active
- 2015-05-19 KR KR1020167035369A patent/KR102343605B1/ko active Active
- 2015-05-19 BR BR112016027063A patent/BR112016027063A2/pt not_active Application Discontinuation
- 2015-05-19 EP EP24208177.6A patent/EP4512908A3/en active Pending
- 2015-05-19 CN CN202111356156.XA patent/CN114032234A/zh active Pending
- 2015-05-19 ES ES15796383T patent/ES2861353T3/es active Active
- 2015-05-19 EP EP21150302.4A patent/EP3901278B1/en active Active
- 2015-05-19 AU AU2015264357A patent/AU2015264357B2/en active Active
- 2015-05-19 WO PCT/US2015/031476 patent/WO2015179339A1/en not_active Ceased
- 2015-05-19 JP JP2016567863A patent/JP2017515484A/ja active Pending
- 2015-05-19 RU RU2016149307A patent/RU2708992C2/ru active
- 2015-05-19 EP EP15796383.6A patent/EP3146080B1/en active Active
- 2015-05-19 CN CN201580039304.1A patent/CN106661627B/zh active Active
- 2015-05-19 CA CA2985631A patent/CA2985631A1/en active Pending
-
2016
- 2016-11-18 US US15/355,235 patent/US20170067090A1/en not_active Abandoned
-
2020
- 2020-09-30 JP JP2020165028A patent/JP7126718B2/ja active Active
-
2021
- 2021-11-11 AU AU2021266291A patent/AU2021266291C1/en active Active
- 2021-11-29 US US17/536,204 patent/US20220090168A1/en active Pending
-
2022
- 2022-08-08 JP JP2022126459A patent/JP2022166131A/ja active Pending
-
2024
- 2024-09-06 JP JP2024153764A patent/JP2024167406A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2024167406A5 (enExample) | ||
| JP7126718B2 (ja) | 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 | |
| JP2017515484A5 (enExample) | ||
| Jäger et al. | Polymerase chain reaction | |
| US7892732B2 (en) | Method of performing PCR amplification on a microarray | |
| JP5624044B2 (ja) | 改善されたアレル−特異的増幅 | |
| CN106912197A (zh) | 用于多重pcr的方法和组合物 | |
| CN100516234C (zh) | Headloop DNA扩增 | |
| JP2005518216A (ja) | 融解温度依存ｄｎａ増幅 | |
| WO2009119331A1 (ja) | サイトケラチン７のｍＲＮＡの検出用プライマを含む試薬 | |
| WO2022138899A1 (ja) | 核酸増幅方法、核酸増幅方法用プライマーセット、プローブ、及びキット | |
| JP7306722B2 (ja) | プライマー及びその使用 | |
| JP4455168B2 (ja) | Ｄｎａ増幅方法 | |
| Ableitner | “Molbio” in Keywords | |
| JP4395363B2 (ja) | 核酸検出方法 | |
| US20220098641A1 (en) | Method for indicating the progress of amplification of nucleic acids and kit for performing the same | |
| KAUL et al. | POLYMERASE CHAIN REACTION: PRINCIPLE, PROCEDURE AND | |
| JP2020535844A (ja) | 核酸の増幅における可逆的な熱力学的トラップ（サーモトラップ） | |
| HK40065933A (en) | Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides | |
| JPWO2022002958A5 (enExample) | ||
| JPWO2023039228A5 (enExample) | ||
| HK1236578A1 (en) | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides | |
| Kanu Jain et al. | Polymerase Chain Reaction: A Powerful Diagnostic and Research Tool | |
| HK1236578B (zh) | 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增 | |
| JP2010088355A (ja) | プライマー試薬およびそれを用いた核酸増幅方法 |