JPWO2022002958A5 - - Google Patents
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- Publication number
- JPWO2022002958A5 JPWO2022002958A5 JP2022580318A JP2022580318A JPWO2022002958A5 JP WO2022002958 A5 JPWO2022002958 A5 JP WO2022002958A5 JP 2022580318 A JP2022580318 A JP 2022580318A JP 2022580318 A JP2022580318 A JP 2022580318A JP WO2022002958 A5 JPWO2022002958 A5 JP WO2022002958A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- nucleotide
- sequence
- acid sequence
- interest
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical group 0.000 claims 44
- 125000003729 nucleotide group Chemical group 0.000 claims 36
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 30
- 239000002773 nucleotide Substances 0.000 claims 28
- 108091034117 Oligonucleotide Proteins 0.000 claims 22
- 238000000034 method Methods 0.000 claims 21
- 238000002844 melting Methods 0.000 claims 13
- 230000008018 melting Effects 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 13
- 230000002209 hydrophobic effect Effects 0.000 claims 11
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 10
- 230000000295 complement effect Effects 0.000 claims 8
- 239000000138 intercalating agent Substances 0.000 claims 6
- 239000000178 monomer Substances 0.000 claims 6
- 238000000137 annealing Methods 0.000 claims 5
- 229940104302 cytosine Drugs 0.000 claims 5
- 230000004049 epigenetic modification Effects 0.000 claims 5
- 239000007858 starting material Substances 0.000 claims 5
- 230000003321 amplification Effects 0.000 claims 4
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 4
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 3
- 229910052739 hydrogen Inorganic materials 0.000 claims 3
- 239000001257 hydrogen Substances 0.000 claims 3
- 125000005647 linker group Chemical group 0.000 claims 3
- 230000011987 methylation Effects 0.000 claims 3
- 238000007069 methylation reaction Methods 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 238000011282 treatment Methods 0.000 claims 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims 1
- 229910000397 disodium phosphate Inorganic materials 0.000 claims 1
- 235000019800 disodium phosphate Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000001976 enzyme digestion Methods 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 238000011269 treatment regimen Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20182947.0 | 2020-06-29 | ||
| EP20182947 | 2020-06-29 | ||
| PCT/EP2021/067880 WO2022002958A1 (en) | 2020-06-29 | 2021-06-29 | Detection of methylation status |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023540161A JP2023540161A (ja) | 2023-09-22 |
| JPWO2022002958A5 true JPWO2022002958A5 (enExample) | 2024-07-03 |
Family
ID=71401636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022580318A Pending JP2023540161A (ja) | 2020-06-29 | 2021-06-29 | メチル化状態の検出 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20250346955A1 (enExample) |
| EP (1) | EP4172361B1 (enExample) |
| JP (1) | JP2023540161A (enExample) |
| KR (1) | KR20230034959A (enExample) |
| CN (1) | CN116209775A (enExample) |
| AU (1) | AU2021302551A1 (enExample) |
| CA (1) | CA3183054A1 (enExample) |
| WO (1) | WO2022002958A1 (enExample) |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6017704A (en) * | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| CN1653079B (zh) | 2001-12-18 | 2010-06-16 | 人类遗传标记控股有限公司 | 包括嵌入剂的假核苷酸 |
| AU2003900368A0 (en) * | 2003-01-24 | 2003-02-13 | Human Genetic Signatures Pty Ltd | Assay for nucleic acid molecules |
| WO2007104318A2 (en) * | 2006-03-16 | 2007-09-20 | Pentabase Aps | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
| GB0714058D0 (en) * | 2007-07-19 | 2007-08-29 | Abertec Ltd | detection of methylation in nucleic acid sequences |
| US20110171642A1 (en) * | 2008-02-27 | 2011-07-14 | Peter Maccallum Cancer Institute | Analysing Methylation Specific PCR by Amplicon Melting |
| KR20130113476A (ko) * | 2010-10-27 | 2013-10-15 | ?티백트 에이/에스 | 인터칼레이터 （ｉｎｔｅｒｃａｌａｔｏｒ） 분자를 포함하는 프로브에 의한 표적 ＤＮＡ 및 ＲＮＡ의 캡쳐 |
| EP3350347B1 (en) * | 2015-09-14 | 2021-01-20 | Pentabase APS | Methods and materials for detection of mutations |
-
2021
- 2021-06-29 JP JP2022580318A patent/JP2023540161A/ja active Pending
- 2021-06-29 EP EP21737437.0A patent/EP4172361B1/en active Active
- 2021-06-29 AU AU2021302551A patent/AU2021302551A1/en active Pending
- 2021-06-29 CN CN202180045918.6A patent/CN116209775A/zh active Pending
- 2021-06-29 KR KR1020227044735A patent/KR20230034959A/ko active Pending
- 2021-06-29 US US18/001,601 patent/US20250346955A1/en active Pending
- 2021-06-29 WO PCT/EP2021/067880 patent/WO2022002958A1/en not_active Ceased
- 2021-06-29 CA CA3183054A patent/CA3183054A1/en active Pending
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