DK3146080T3 - Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides - Google Patents
Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides Download PDFInfo
- Publication number
- DK3146080T3 DK3146080T3 DK15796383.6T DK15796383T DK3146080T3 DK 3146080 T3 DK3146080 T3 DK 3146080T3 DK 15796383 T DK15796383 T DK 15796383T DK 3146080 T3 DK3146080 T3 DK 3146080T3
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- Prior art keywords
- allele
- specific
- composition
- blocker oligonucleotides
- overlapping non
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Links
- 108700028369 Alleles Proteins 0.000 title 3
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462000114P | 2014-05-19 | 2014-05-19 | |
| PCT/US2015/031476 WO2015179339A1 (en) | 2014-05-19 | 2015-05-19 | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3146080T3 true DK3146080T3 (en) | 2021-04-12 |
Family
ID=54554607
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15796383.6T DK3146080T3 (en) | 2014-05-19 | 2015-05-19 | Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20170067090A1 (enExample) |
| EP (3) | EP3146080B1 (enExample) |
| JP (4) | JP2017515484A (enExample) |
| KR (1) | KR102343605B1 (enExample) |
| CN (2) | CN114032234A (enExample) |
| AU (2) | AU2015264357B2 (enExample) |
| BR (1) | BR112016027063A2 (enExample) |
| CA (1) | CA2985631A1 (enExample) |
| DK (1) | DK3146080T3 (enExample) |
| ES (1) | ES2861353T3 (enExample) |
| PH (1) | PH12016502542B1 (enExample) |
| RU (1) | RU2708992C2 (enExample) |
| WO (1) | WO2015179339A1 (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| WO2013130674A1 (en) | 2012-02-27 | 2013-09-06 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| ES2711168T3 (es) | 2013-08-28 | 2019-04-30 | Becton Dickinson Co | Análisis masivo en paralelo de células individuales |
| US10801070B2 (en) | 2013-11-25 | 2020-10-13 | The Broad Institute, Inc. | Compositions and methods for diagnosing, evaluating and treating cancer |
| WO2015085147A1 (en) | 2013-12-05 | 2015-06-11 | The Broad Institute Inc. | Polymorphic gene typing and somatic change detection using sequencing data |
| BR112016014410A2 (pt) | 2013-12-20 | 2018-02-20 | The Broad Institute Inc. | terapia de combinação com vacina de neoantígeno |
| WO2016100975A1 (en) | 2014-12-19 | 2016-06-23 | Massachsetts Institute Ot Technology | Molecular biomarkers for cancer immunotherapy |
| US10993997B2 (en) | 2014-12-19 | 2021-05-04 | The Broad Institute, Inc. | Methods for profiling the t cell repertoire |
| EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
| CN107406888A (zh) | 2015-03-30 | 2017-11-28 | 赛卢拉研究公司 | 用于组合条形编码的方法和组合物 |
| US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
| WO2017044574A1 (en) | 2015-09-11 | 2017-03-16 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
| CN108603193B (zh) | 2015-12-30 | 2022-07-22 | 生物辐射实验室股份有限公司 | 分裂循环和tape扩增 |
| WO2017193025A1 (en) | 2016-05-06 | 2017-11-09 | William Marsh Rice University | Stoichiometric nucleic acid purification using randomer capture probe libraries |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| IL265478B2 (en) | 2016-09-26 | 2024-10-01 | Becton Dickinson Co | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| WO2018140391A1 (en) * | 2017-01-24 | 2018-08-02 | The Broad Institute, Inc. | Compositions and methods for detecting a mutant variant of a polynucleotide |
| WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| US11795485B2 (en) | 2017-10-18 | 2023-10-24 | Day Zero Diagnostics, Inc. | Selective enrichment of a population of DNA in a mixed DNA sample through targeted suppression of DNA amplification |
| US12331350B2 (en) * | 2018-02-20 | 2025-06-17 | William Marsh Rice University | Systems and methods for allele enrichment using multiplexed blocker displacement amplification |
| CN108220444A (zh) * | 2018-02-28 | 2018-06-29 | 无锡禾盛医疗器械有限公司 | 一种kras基因突变检测的引物探针组合及其应用 |
| US11365409B2 (en) | 2018-05-03 | 2022-06-21 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| CN112969789A (zh) | 2018-11-08 | 2021-06-15 | 贝克顿迪金森公司 | 使用随机引发的单细胞全转录组分析 |
| WO2020123384A1 (en) * | 2018-12-13 | 2020-06-18 | Cellular Research, Inc. | Selective extension in single cell whole transcriptome analysis |
| US20200224260A1 (en) * | 2019-01-15 | 2020-07-16 | Tangen Bioscience Inc. | Method for suppressing non-specific amplification products in nucleic acid amplification technologies |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| US20220348606A1 (en) * | 2019-06-26 | 2022-11-03 | Nanjing GenScript Biotech Co., Ltd. | Oligonucleotide containing blocker |
| WO2021016239A1 (en) | 2019-07-22 | 2021-01-28 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
| WO2021125854A1 (ko) * | 2019-12-20 | 2021-06-24 | 고려대학교 산학협력단 | 극소량의 희귀 단일 염기 변이체 검출용 프라이머 및 이를 이용하여 극소량의 희귀 단일 염기 변이체를 특이적이고 민감하게 검출하는 방법 |
| CN115244184A (zh) | 2020-01-13 | 2022-10-25 | 贝克顿迪金森公司 | 用于定量蛋白和rna的方法和组合物 |
| CN115335520A (zh) | 2020-01-29 | 2022-11-11 | 贝克顿迪金森公司 | 用于通过测序对单细胞进行空间映射的条形码化的孔 |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| CA3176980A1 (en) * | 2020-05-01 | 2021-11-04 | William Marsh Rice University | Quantitative blocker displacement amplification (qbda) sequencing for calibration-free and multiplexed variant allele frequency quantitation |
| WO2021231779A1 (en) | 2020-05-14 | 2021-11-18 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| EP4407030B1 (en) | 2020-06-02 | 2025-12-17 | Becton, Dickinson and Company | Oligonucleotides and beads for 5 prime gene expression assay |
| CN116075520A (zh) * | 2020-06-26 | 2023-05-05 | 威廉马歇莱思大学 | 扩增子全面富集 |
| CN111647650B (zh) * | 2020-07-06 | 2023-06-27 | 河南赛诺特生物技术有限公司 | 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| EP4189112A1 (en) | 2020-07-31 | 2023-06-07 | Becton Dickinson and Company | Single cell assay for transposase-accessible chromatin |
| US20230399687A1 (en) * | 2020-11-02 | 2023-12-14 | Nuprobe Usa, Inc. | Quantitative Multiplex Amplicon Sequencing System |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| CN112522369A (zh) * | 2020-12-08 | 2021-03-19 | 合肥欧创基因生物科技有限公司 | 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法 |
| EP4263859A1 (en) | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
| WO2022146773A1 (en) | 2020-12-29 | 2022-07-07 | Nuprobe Usa, Inc. | Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta) |
| EP4278009A1 (en) | 2021-01-15 | 2023-11-22 | Nuprobe USA, Inc. | Cycle multiplexing for highly multiplexed quantitative pcr |
| WO2022197688A1 (en) | 2021-03-16 | 2022-09-22 | Nuprobe Usa, Inc. | High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection |
| WO2023077121A1 (en) * | 2021-11-01 | 2023-05-04 | Nuprobe Usa, Inc. | Rna quantitative amplicon sequencing for gene expression quantitation |
| CN119662835B (zh) * | 2025-02-20 | 2025-05-27 | 宁波熙宁检测技术有限公司 | 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6312892B1 (en) * | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
| US7517977B2 (en) * | 2004-10-18 | 2009-04-14 | Brandeis University | Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification |
| US20100009355A1 (en) * | 2006-03-14 | 2010-01-14 | Kolodney Michael S | Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides |
| US8071338B2 (en) * | 2007-08-08 | 2011-12-06 | Roche Molecular Systems, Inc. | Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity |
| EP2207896B1 (en) * | 2007-10-04 | 2014-01-29 | Commonwealth Scientific and Industrial Research Organisation | Nucleic acid amplification |
| ES2527653T3 (es) * | 2008-10-20 | 2015-01-28 | F. Hoffmann-La Roche Ag | Amplificación específica de alelo usando un cebador con un nucleótido modificado |
| US8206929B2 (en) * | 2009-01-07 | 2012-06-26 | Roche Molecular Systems, Inc. | Nucleic acid amplification with allele-specific suppression of sequence variants |
| CN102428190B (zh) * | 2009-03-27 | 2014-02-26 | 生命技术公司 | 用于检测等位基因变体的方法、组合物和试剂盒 |
| KR101312241B1 (ko) * | 2010-04-27 | 2013-09-27 | 사회복지법인 삼성생명공익재단 | 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법 |
| WO2011146403A2 (en) * | 2010-05-16 | 2011-11-24 | Reniguard Life Sciences, Inc. | Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use |
| EP2663651B1 (en) * | 2011-01-14 | 2018-06-20 | Genefirst Limited | Methods, compositions, and kits for determing the presence/absence of a variant nucleic acid sequence |
| JP6404118B2 (ja) * | 2011-05-04 | 2018-10-10 | バイオセプト インコーポレイティッド | 核酸配列変種を検出するための方法 |
| EP2798078B1 (en) * | 2011-12-30 | 2018-02-14 | Polowang Biotech Inc. | Method and primer set for detecting mutation |
| EP2841598B1 (en) * | 2012-04-25 | 2018-03-14 | Ho, Tho Huu | Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products |
| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
| JP6652693B2 (ja) * | 2013-04-29 | 2020-02-26 | キアゲン ゲーエムベーハー | ブロッキングオリゴヌクレオチドを用いたｄｎａ増幅の方法 |
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2015
- 2015-05-19 KR KR1020167035369A patent/KR102343605B1/ko active Active
- 2015-05-19 EP EP15796383.6A patent/EP3146080B1/en active Active
- 2015-05-19 PH PH1/2016/502542A patent/PH12016502542B1/en unknown
- 2015-05-19 AU AU2015264357A patent/AU2015264357B2/en active Active
- 2015-05-19 JP JP2016567863A patent/JP2017515484A/ja active Pending
- 2015-05-19 CN CN202111356156.XA patent/CN114032234A/zh active Pending
- 2015-05-19 EP EP21150302.4A patent/EP3901278B1/en active Active
- 2015-05-19 CN CN201580039304.1A patent/CN106661627B/zh active Active
- 2015-05-19 DK DK15796383.6T patent/DK3146080T3/da active
- 2015-05-19 BR BR112016027063A patent/BR112016027063A2/pt not_active Application Discontinuation
- 2015-05-19 RU RU2016149307A patent/RU2708992C2/ru active
- 2015-05-19 CA CA2985631A patent/CA2985631A1/en active Pending
- 2015-05-19 WO PCT/US2015/031476 patent/WO2015179339A1/en not_active Ceased
- 2015-05-19 EP EP24208177.6A patent/EP4512908A3/en active Pending
- 2015-05-19 ES ES15796383T patent/ES2861353T3/es active Active
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2016
- 2016-11-18 US US15/355,235 patent/US20170067090A1/en not_active Abandoned
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2020
- 2020-09-30 JP JP2020165028A patent/JP7126718B2/ja active Active
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2021
- 2021-11-11 AU AU2021266291A patent/AU2021266291C1/en active Active
- 2021-11-29 US US17/536,204 patent/US20220090168A1/en active Pending
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2022
- 2022-08-08 JP JP2022126459A patent/JP2022166131A/ja active Pending
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2024
- 2024-09-06 JP JP2024153764A patent/JP2024167406A/ja active Pending
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