JP2017503517A5 - - Google Patents

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JP2017503517A5
JP2017503517A5 JP2016547868A JP2016547868A JP2017503517A5 JP 2017503517 A5 JP2017503517 A5 JP 2017503517A5 JP 2016547868 A JP2016547868 A JP 2016547868A JP 2016547868 A JP2016547868 A JP 2016547868A JP 2017503517 A5 JP2017503517 A5 JP 2017503517A5
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Japan
Prior art keywords
polynucleotide
target polynucleotide
loading
binding proteins
pore
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JP2016547868A
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English (en)
Japanese (ja)
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JP2017503517A (ja
JP6749243B2 (ja
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Priority claimed from PCT/GB2014/050175 external-priority patent/WO2014135838A1/en
Priority claimed from GBGB1406151.9A external-priority patent/GB201406151D0/en
Priority claimed from GBGB1406155.0A external-priority patent/GB201406155D0/en
Priority claimed from GB201416197A external-priority patent/GB201416197D0/en
Application filed filed Critical
Priority claimed from PCT/GB2015/050140 external-priority patent/WO2015110813A1/en
Publication of JP2017503517A publication Critical patent/JP2017503517A/ja
Publication of JP2017503517A5 publication Critical patent/JP2017503517A5/ja
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JP2016547868A 2014-01-22 2015-01-22 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 Active JP6749243B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
GBPCT/GB2014/050175 2014-01-22
PCT/GB2014/050175 WO2014135838A1 (en) 2013-03-08 2014-01-22 Enzyme stalling method
GBGB1406151.9A GB201406151D0 (en) 2014-04-04 2014-04-04 Method
GB1406155.0 2014-04-04
GB1406151.9 2014-04-04
GBGB1406155.0A GB201406155D0 (en) 2014-04-04 2014-04-04 Method
GB1416197.0 2014-09-12
GB201416197A GB201416197D0 (en) 2014-09-12 2014-09-12 Method
PCT/GB2015/050140 WO2015110813A1 (en) 2014-01-22 2015-01-22 Method for attaching one or more polynucleotide binding proteins to a target polynucleotide

Publications (3)

Publication Number Publication Date
JP2017503517A JP2017503517A (ja) 2017-02-02
JP2017503517A5 true JP2017503517A5 (enExample) 2018-03-08
JP6749243B2 JP6749243B2 (ja) 2020-09-02

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JP2016547868A Active JP6749243B2 (ja) 2014-01-22 2015-01-22 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法

Country Status (7)

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US (3) US10385389B2 (enExample)
EP (1) EP3097210B2 (enExample)
JP (1) JP6749243B2 (enExample)
CN (2) CN111534504B (enExample)
AU (1) AU2015208919B9 (enExample)
CA (1) CA2937411C (enExample)
WO (1) WO2015110813A1 (enExample)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
JP6614972B2 (ja) 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
JP7237586B2 (ja) 2015-12-08 2023-03-13 カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント 修飾ナノポア、それを含む組成物およびその使用
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
CA3020203A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201609220D0 (en) * 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN109196116B (zh) 2016-05-25 2022-09-27 牛津纳米孔科技公开有限公司 一种表征靶多核苷酸的方法
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
EP3580228B1 (en) * 2017-02-10 2021-07-28 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
GB201707140D0 (en) * 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
JP2020031557A (ja) * 2018-08-28 2020-03-05 株式会社日立ハイテクノロジーズ 生体分子分析装置
JP6545888B1 (ja) * 2018-11-09 2019-07-17 国立大学法人東北大学 情報処理システム、公開鍵の変更方法、およびプログラム
GB201907244D0 (en) * 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
GB2602736A (en) * 2019-09-18 2022-07-13 Hitachi High Tech Corp Adapter molecule, biomolecule-adapter molecule complex in which said adapter molecule and biomolecule are bound, biomolecule analysis apparatus, and
US20230220450A1 (en) * 2019-09-18 2023-07-13 Hitachi High-Tech Corporation Adapter molecule, biomolecule-adapter molecule complex composed of adapter molecule and biomolecule bound together, biomolecule analyzer and biomolecule analysis method
WO2021056598A1 (zh) 2019-09-29 2021-04-01 北京齐碳科技有限公司 一种Mmup单体变体及其应用
WO2021056599A1 (zh) * 2019-09-29 2021-04-01 北京齐碳科技有限公司 一种Mnep单体变体及其应用
IL291788A (en) 2019-09-29 2022-07-01 Qitan Tech Ltd Beijing mnep monomer variant and its application
US20230295712A1 (en) * 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
GB202009349D0 (en) * 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
CN115968410A (zh) * 2020-06-18 2023-04-14 牛津纳米孔科技公开有限公司 表征移动穿过纳米孔的多核苷酸的方法
EP4193363A1 (en) 2020-08-07 2023-06-14 Oxford Nanopore Technologies plc Methods of identifying nucleic acid barcodes
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
CN112851771A (zh) * 2021-02-09 2021-05-28 中国科学院武汉病毒研究所 连接核酸与蛋白或肽的方法
CN112851765B (zh) * 2021-02-09 2023-02-28 中国科学院武汉病毒研究所 蛋白或肽与核酸共价连接的方法
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN113862264A (zh) * 2021-12-03 2021-12-31 北京齐碳科技有限公司 用于靶多核苷酸测序的衔接子、构建体、方法和应用
CN114262735B (zh) * 2021-12-15 2025-09-16 成都齐碳科技有限公司 用于表征多核苷酸的衔接体及其用途
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
CN114854826A (zh) * 2022-05-13 2022-08-05 北京齐碳科技有限公司 序列、包含序列的接头及其用途
AU2023270700A1 (en) 2022-05-17 2024-10-31 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
EP4526440A1 (en) 2022-05-18 2025-03-26 Oxford Nanopore Technologies PLC Compositions and methods for nucleic acid extraction and purification
CN114836452B (zh) * 2022-05-19 2023-12-05 浙江安诺优达生物科技有限公司 一种密码子优化的核酸外切酶ⅲ基因及其表达方法
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
CN120344725A (zh) * 2022-12-26 2025-07-18 深圳华大生命科学研究院 减少测序文库中游离接头的方法
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
GB202307494D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Ltd Nanopore sequencing and preparation modular instrument
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
WO2025241113A1 (zh) * 2024-05-22 2025-11-27 深圳华大生命科学研究院 控制多核苷酸移动的方法及解旋酶变体

Family Cites Families (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP3891620B2 (ja) 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US20020197614A1 (en) * 1997-05-16 2002-12-26 Mosaic Technologies, Inc. Electrophoretic analysis of target molecules using adapter molecules
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6150112A (en) * 1998-09-18 2000-11-21 Yale University Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
AU5763000A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Molecular and atomic scale evaluation of biopolymers
CA2381139A1 (en) 1999-08-31 2001-03-08 Michael Niederweis Method for the production of a channel forming protein
US6916665B2 (en) 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
AU2001243232A1 (en) * 2000-02-25 2001-09-03 University Of South Carolina Research Foundation Topoisomerase linker-mediated amplification methods
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
EP1606417A2 (en) * 2003-03-07 2005-12-21 Rubicon Genomics Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
DE602004025801D1 (de) * 2003-03-25 2010-04-15 Stratagene California Dna-polymerasefusionen und deren verwendungen
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
AU2003904237A0 (en) 2003-08-08 2003-08-21 Garvan Institute Of Medical Research Novel translocation assay
WO2005076010A2 (en) 2004-02-06 2005-08-18 Council Of Scientific And Industrial Research Computational method for identifying adhesin and adhesin-like proteins of therapeutic potential
JP2005253427A (ja) * 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 dna 검출방법 및검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
CA2633476C (en) 2005-12-22 2015-04-21 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7849581B2 (en) 2006-05-05 2010-12-14 University Of Utah Research Foundation Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof
US7638034B2 (en) 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
US8500982B2 (en) 2007-04-04 2013-08-06 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
EP3045542B1 (en) 2008-03-28 2016-11-16 Pacific Biosciences of California, Inc. Methods for nucleic acid sequencing
JP2012500620A (ja) 2008-04-24 2012-01-12 ザ トラスティーズ オブ コロンビア ユニバーシティー イン ザ シティー オブ ニューヨーク Dna分子組織化のための幾何学的パターンおよび脂質二重層、ならびにその使用
US8772041B2 (en) 2008-05-22 2014-07-08 The Regents Of The University Of California Membrane precursors and membranes formed therefrom
US8652771B2 (en) 2008-05-28 2014-02-18 University of Souther California Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
ES2576114T3 (es) 2008-09-22 2016-07-05 University Of Washington Nanoporos MSP y procedimientos relacionados
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
BRPI1007215A2 (pt) 2009-01-30 2017-08-29 Oxford Nanopore Tech Ltd Método de acoplamento covalente de duas ou mais porções, primeira e segunda porções, primeiro porção acoplada a uma segunda porção, par de primeiro e segundo ligantes, e, uso de um par de ligantes.
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
CA2781581C (en) 2009-12-01 2018-03-06 Oxford Nanopore Technologies Limited Biochemical analysis instrument
ES2892158T3 (es) 2010-02-23 2022-02-02 Univ Washington Membranas artificiales de ácidos micólicos
US9073990B2 (en) 2010-04-05 2015-07-07 Bar-Llan University Protease-activatable pore-forming polypeptides
US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
CN102116783B (zh) 2010-12-31 2013-05-29 北京普源精电科技有限公司 一种波形显示方法
CN102174554A (zh) 2011-01-24 2011-09-07 内蒙古民族大学 双控双调节原核表达载体系统及其构建方法和用途
US20120196279A1 (en) 2011-02-02 2012-08-02 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
EP3848706B1 (en) 2011-05-27 2023-07-19 Oxford Nanopore Technologies PLC Coupling method
WO2012166906A1 (en) 2011-05-31 2012-12-06 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
BR112014001699A2 (pt) 2011-07-25 2017-06-13 Oxford Nanopore Tech Ltd método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
KR20140090633A (ko) 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
GB201120910D0 (en) 2011-12-06 2012-01-18 Cambridge Entpr Ltd Nanopore functionality control
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2798084B1 (en) 2011-12-29 2017-04-19 Oxford Nanopore Technologies Limited Enzyme method
WO2013109970A1 (en) * 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
CA2869546C (en) 2012-04-10 2020-07-21 Oxford Nanopore Technologies Limited Mutant lysenin pores
TWI655213B (zh) 2012-07-13 2019-04-01 Menicon Co., Ltd. 自我組織化肽衍生物的製造方法
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
JP6614972B2 (ja) * 2012-07-19 2019-12-04 オックスフォード ナノポール テクノロジーズ リミテッド 修飾ヘリカーゼ
WO2014064444A1 (en) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US10179933B2 (en) 2013-02-07 2019-01-15 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Hybrid nanopores and uses thereof for detection of analytes
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
US10613076B2 (en) 2013-03-14 2020-04-07 The Trustees Of Boston University Optoelectronic control of solid-state nanopores
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CA2907423A1 (en) 2013-05-24 2014-11-27 Illumina Cambridge Limited Pyrophosphorolytic sequencing
US20150011398A1 (en) * 2013-06-17 2015-01-08 Kim Lewis Methods for quantitative determination of protein-nucleic acid interactions in complex mixtures
WO2015051378A1 (en) 2013-10-04 2015-04-09 University Of Washington Through Its Center For Commercialization Systems and methods for nanopore-based analysis of nucleic acids
CN118086476A (zh) 2013-10-18 2024-05-28 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
US9689033B2 (en) 2013-11-26 2017-06-27 Illumina, Inc. Compositions and methods for polynucleotide sequencing
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
FR3023394B1 (fr) 2014-07-02 2017-12-29 Adn Access Data Networks Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
JP7237586B2 (ja) 2015-12-08 2023-03-13 カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント 修飾ナノポア、それを含む組成物およびその使用
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
CA3020203A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔

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