JP2017503517A5 - - Google Patents

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JP2017503517A5
JP2017503517A5 JP2016547868A JP2016547868A JP2017503517A5 JP 2017503517 A5 JP2017503517 A5 JP 2017503517A5 JP 2016547868 A JP2016547868 A JP 2016547868A JP 2016547868 A JP2016547868 A JP 2016547868A JP 2017503517 A5 JP2017503517 A5 JP 2017503517A5
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polynucleotide
target polynucleotide
loading
binding proteins
pore
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JP2016547868A
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English (en)
Japanese (ja)
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JP2017503517A (ja
JP6749243B2 (ja
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Priority claimed from PCT/GB2014/050175 external-priority patent/WO2014135838A1/en
Priority claimed from GBGB1406151.9A external-priority patent/GB201406151D0/en
Priority claimed from GBGB1406155.0A external-priority patent/GB201406155D0/en
Priority claimed from GB201416197A external-priority patent/GB201416197D0/en
Application filed filed Critical
Priority claimed from PCT/GB2015/050140 external-priority patent/WO2015110813A1/en
Publication of JP2017503517A publication Critical patent/JP2017503517A/ja
Publication of JP2017503517A5 publication Critical patent/JP2017503517A5/ja
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JP2016547868A 2014-01-22 2015-01-22 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 Active JP6749243B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
PCT/GB2014/050175 WO2014135838A1 (en) 2013-03-08 2014-01-22 Enzyme stalling method
GBPCT/GB2014/050175 2014-01-22
GBGB1406151.9A GB201406151D0 (en) 2014-04-04 2014-04-04 Method
GB1406155.0 2014-04-04
GB1406151.9 2014-04-04
GBGB1406155.0A GB201406155D0 (en) 2014-04-04 2014-04-04 Method
GB1416197.0 2014-09-12
GB201416197A GB201416197D0 (en) 2014-09-12 2014-09-12 Method
PCT/GB2015/050140 WO2015110813A1 (en) 2014-01-22 2015-01-22 Method for attaching one or more polynucleotide binding proteins to a target polynucleotide

Publications (3)

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JP2017503517A JP2017503517A (ja) 2017-02-02
JP2017503517A5 true JP2017503517A5 (enExample) 2018-03-08
JP6749243B2 JP6749243B2 (ja) 2020-09-02

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JP2016547868A Active JP6749243B2 (ja) 2014-01-22 2015-01-22 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法

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US (3) US10385389B2 (enExample)
EP (1) EP3097210B2 (enExample)
JP (1) JP6749243B2 (enExample)
CN (2) CN111534504B (enExample)
AU (1) AU2015208919B9 (enExample)
CA (1) CA2937411C (enExample)
WO (1) WO2015110813A1 (enExample)

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN117965707A (zh) 2013-10-18 2024-05-03 牛津纳米孔科技公开有限公司 经修饰的酶
AU2015208919B9 (en) 2014-01-22 2021-04-01 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015166275A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Mutant pores
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
CN108779170A (zh) 2016-03-02 2018-11-09 牛津纳米孔技术公司 突变孔
AU2017246690B2 (en) 2016-04-06 2022-03-24 Oxford Nanopore Technologies Plc Mutant pore
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP4063521B1 (en) 2016-05-25 2024-02-21 Oxford Nanopore Technologies PLC Method of nanopore sequencing
GB201609220D0 (en) * 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
AU2018218531B2 (en) * 2017-02-10 2021-01-28 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CA3066715A1 (en) 2017-06-30 2019-01-03 Vib Vzw Novel protein pores
GB2569977A (en) * 2018-01-05 2019-07-10 Oxford Nanopore Tech Ltd Method
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
JP2020031557A (ja) * 2018-08-28 2020-03-05 株式会社日立ハイテクノロジーズ 生体分子分析装置
JP6545888B1 (ja) * 2018-11-09 2019-07-17 国立大学法人東北大学 情報処理システム、公開鍵の変更方法、およびプログラム
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US11926819B2 (en) 2019-05-28 2024-03-12 The Regents Of The University Of California Methods of adding polymers to ribonucleic acids
GB2602736A (en) * 2019-09-18 2022-07-13 Hitachi High Tech Corp Adapter molecule, biomolecule-adapter molecule complex in which said adapter molecule and biomolecule are bound, biomolecule analysis apparatus, and
GB2603061A (en) * 2019-09-18 2022-07-27 Hitachi High Tech Corp Adapter molecule, biomolecule-adapter molecule complex composed of adapter molecule and biomolecule bound together, biomolecule analyzer and biomolecule
WO2021056598A1 (zh) 2019-09-29 2021-04-01 北京齐碳科技有限公司 一种Mmup单体变体及其应用
WO2021056599A1 (zh) * 2019-09-29 2021-04-01 北京齐碳科技有限公司 一种Mnep单体变体及其应用
WO2021255475A1 (en) * 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited A method of selectively characterising a polynucleotide using a detector
WO2021255476A2 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited Method
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
US20220059187A1 (en) 2020-08-07 2022-02-24 Oxford Nanopore Technologies Limited Methods of detecting nucleic acid barcodes
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
CN112851765B (zh) * 2021-02-09 2023-02-28 中国科学院武汉病毒研究所 蛋白或肽与核酸共价连接的方法
CN112851771A (zh) * 2021-02-09 2021-05-28 中国科学院武汉病毒研究所 连接核酸与蛋白或肽的方法
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
CN114134142B (zh) * 2021-11-23 2024-05-28 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN113862264A (zh) * 2021-12-03 2021-12-31 北京齐碳科技有限公司 用于靶多核苷酸测序的衔接子、构建体、方法和应用
CN114262735B (zh) * 2021-12-15 2025-09-16 成都齐碳科技有限公司 用于表征多核苷酸的衔接体及其用途
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
CN114854826A (zh) * 2022-05-13 2022-08-05 北京齐碳科技有限公司 序列、包含序列的接头及其用途
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
US20250304947A1 (en) 2022-05-18 2025-10-02 Oxford Nanopore Technologies plc. Compositions and methods for nucleic acid extraction and purification
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
CN114836452B (zh) * 2022-05-19 2023-12-05 浙江安诺优达生物科技有限公司 一种密码子优化的核酸外切酶ⅲ基因及其表达方法
CN116478983B (zh) * 2022-11-21 2023-10-24 北京普译生物科技有限公司 一种rna-dna嵌合接头及其应用
WO2024138320A1 (zh) * 2022-12-26 2024-07-04 深圳华大生命科学研究院 减少测序文库中游离接头的方法
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202307494D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Ltd Nanopore sequencing and preparation modular instrument
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method

Family Cites Families (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP3891620B2 (ja) 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US20020197614A1 (en) * 1997-05-16 2002-12-26 Mosaic Technologies, Inc. Electrophoretic analysis of target molecules using adapter molecules
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6150112A (en) * 1998-09-18 2000-11-21 Yale University Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
AU5631200A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Control of solid state dimensional features
EP1208207A2 (de) 1999-08-31 2002-05-29 Michael Niederweis Verfahren zur herstellung eines kanalbildenden proteins
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
AU2001243232A1 (en) * 2000-02-25 2001-09-03 University Of South Carolina Research Foundation Topoisomerase linker-mediated amplification methods
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6936433B2 (en) 2000-11-27 2005-08-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
WO2003095669A1 (en) 2002-05-10 2003-11-20 The Texas A & M University System Stochastic sensing through covalent interactions
WO2004081183A2 (en) * 2003-03-07 2004-09-23 Rubicon Genomics, Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
CA2519309A1 (en) * 2003-03-25 2004-10-14 Stratagene California Dna polymerase fusions and uses thereof
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
AU2003904237A0 (en) 2003-08-08 2003-08-21 Garvan Institute Of Medical Research Novel translocation assay
KR101178776B1 (ko) 2004-02-06 2012-09-07 카운슬 오브 사이언티픽 앤드 인더스트리얼 리서치 치료학적 잠재성을 가진 어드헤신 및 어드헤신계 단백질을 동정하기 위한 컴퓨터 장치
JP2005253427A (ja) * 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
EP1842061A4 (en) 2004-12-21 2009-05-13 Texas A & M Univ Sys HIGH-TEMPERATURE ION CHANNELS AND ION PORES
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 dna 검출방법 및검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
AU2006331512B2 (en) 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7849581B2 (en) 2006-05-05 2010-12-14 University Of Utah Research Foundation Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof
US7638034B2 (en) 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
WO2008102121A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
KR101521990B1 (ko) 2007-04-04 2015-05-20 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 나노포어 사용을 위한 조성물, 장치, 시스템 및 방법
EP3543357A1 (en) 2007-05-08 2019-09-25 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
WO2009052214A2 (en) 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
MX2010010600A (es) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Composiciones y metodos para secuenciacion de acidos nucleicos.
WO2009132124A2 (en) 2008-04-24 2009-10-29 The Trustees Of Columbia University In The City Of New York Geometric patterns and lipid bilayers for dna molecule organization and uses thereof
EP2293921A4 (en) 2008-05-22 2013-05-22 Univ California MEMBRANE PROBLEMS AND MEMBRANES MADE FROM THESE
US8652771B2 (en) 2008-05-28 2014-02-18 University of Souther California Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects
CN102245760A (zh) 2008-07-07 2011-11-16 牛津纳米孔技术有限公司 酶-孔构建体
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
CN104710519B (zh) 2008-09-22 2021-05-28 华盛顿大学 Msp纳米微孔和相关方法
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
AU2010209528B2 (en) 2009-01-30 2015-10-01 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US9222082B2 (en) 2009-01-30 2015-12-29 Oxford Nanopore Technologies Limited Hybridization linkers
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
KR20110138286A (ko) 2009-04-20 2011-12-26 옥스포드 나노포어 테크놀로지즈 리미티드 지질 이분자층 센서 어레이
JP5873023B2 (ja) 2009-12-01 2016-03-01 オックスフォード ナノポール テクノロジーズ リミテッド 生化学分析機器
EP3933402A1 (en) 2010-02-23 2022-01-05 University of Washington Method of making unsupported artificial mycolic acid membranes
US9073990B2 (en) 2010-04-05 2015-07-07 Bar-Llan University Protease-activatable pore-forming polypeptides
CN103154729B (zh) 2010-06-08 2015-01-07 哈佛大学校长及研究员协会 具有由石墨烯支持的人工脂质膜的纳米孔装置
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
CN102116783B (zh) 2010-12-31 2013-05-29 北京普源精电科技有限公司 一种波形显示方法
CN102174554A (zh) 2011-01-24 2011-09-07 内蒙古民族大学 双控双调节原核表达载体系统及其构建方法和用途
US20120196279A1 (en) 2011-02-02 2012-08-02 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
CA2826374C (en) 2011-02-11 2024-01-23 Oxford Nanopore Technologies Limited Mutant pores
US8980553B2 (en) * 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
BR112013030529B1 (pt) 2011-05-27 2023-03-07 Oxford Nanopore Technologies Plc Método para determinar a presença, ausência ou características de um analito, método e aparelho para sequenciamento de um analito que é um polinucleotídeo alvo, e, kit
WO2012166906A1 (en) 2011-05-31 2012-12-06 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
JP6298404B2 (ja) * 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
US20160162634A1 (en) 2011-09-23 2016-06-09 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
GB201120910D0 (en) 2011-12-06 2012-01-18 Cambridge Entpr Ltd Nanopore functionality control
BR112014016112B8 (pt) 2011-12-29 2022-12-27 Oxford Nanopore Tech Ltd Método e aparelho para caracterizar um polinucleotídeo alvo, kit, e, uso de uma helicase
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
EP3415901A1 (en) * 2012-01-20 2018-12-19 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
TWI655213B (zh) 2012-07-13 2019-04-01 目立康股份有限公司 自我組織化肽衍生物的製造方法
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
US10808231B2 (en) * 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
JP6429773B2 (ja) * 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
EP2911771B1 (en) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2954320B1 (en) 2013-02-07 2018-04-18 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Hybrid nanopores and uses thereof for detection of analytes
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
CA3094792A1 (en) 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014153047A1 (en) 2013-03-14 2014-09-25 The Trustees Of Boston University Optoelectronic control of solid-state nanopores
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN105264093B (zh) 2013-05-24 2018-04-10 伊鲁米纳剑桥有限公司 焦磷酸解测序
US20150011397A1 (en) * 2013-06-17 2015-01-08 Kim Lewis Methods for quantitative determination of multiple proteins in complex mixtures
WO2015051378A1 (en) 2013-10-04 2015-04-09 University Of Washington Through Its Center For Commercialization Systems and methods for nanopore-based analysis of nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CN117965707A (zh) 2013-10-18 2024-05-03 牛津纳米孔科技公开有限公司 经修饰的酶
JP6800749B2 (ja) 2013-11-26 2020-12-23 イルミナ インコーポレイテッド ポリヌクレオチド配列決定のための組成物及び方法
AU2015208919B9 (en) 2014-01-22 2021-04-01 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
WO2015166275A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Mutant pores
FR3023394B1 (fr) 2014-07-02 2017-12-29 Adn Access Data Networks Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
CN108779170A (zh) 2016-03-02 2018-11-09 牛津纳米孔技术公司 突变孔
AU2017246690B2 (en) 2016-04-06 2022-03-24 Oxford Nanopore Technologies Plc Mutant pore
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CA3066715A1 (en) 2017-06-30 2019-01-03 Vib Vzw Novel protein pores

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