JP2010539966A5 - - Google Patents

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Publication number
JP2010539966A5
JP2010539966A5 JP2010527535A JP2010527535A JP2010539966A5 JP 2010539966 A5 JP2010539966 A5 JP 2010539966A5 JP 2010527535 A JP2010527535 A JP 2010527535A JP 2010527535 A JP2010527535 A JP 2010527535A JP 2010539966 A5 JP2010539966 A5 JP 2010539966A5
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JP
Japan
Prior art keywords
pore
transmembrane protein
analyte
adapter
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2010527535A
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English (en)
Japanese (ja)
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JP2010539966A (ja
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Publication date
Priority claimed from GB0719531A external-priority patent/GB2453377A/en
Application filed filed Critical
Publication of JP2010539966A publication Critical patent/JP2010539966A/ja
Publication of JP2010539966A5 publication Critical patent/JP2010539966A5/ja
Pending legal-status Critical Current

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JP2010527535A 2007-10-05 2008-10-06 分子アダプター Pending JP2010539966A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0719531A GB2453377A (en) 2007-10-05 2007-10-05 Transmembrane protein pores and molecular adapters therefore.
PCT/GB2008/003372 WO2009044170A1 (en) 2007-10-05 2008-10-06 Molecular adaptors

Publications (2)

Publication Number Publication Date
JP2010539966A JP2010539966A (ja) 2010-12-24
JP2010539966A5 true JP2010539966A5 (enExample) 2011-11-24

Family

ID=38739231

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010527535A Pending JP2010539966A (ja) 2007-10-05 2008-10-06 分子アダプター

Country Status (8)

Country Link
US (1) US8822160B2 (enExample)
EP (1) EP2198046B1 (enExample)
JP (1) JP2010539966A (enExample)
AU (1) AU2008306633A1 (enExample)
CA (1) CA2701602A1 (enExample)
GB (1) GB2453377A (enExample)
IL (1) IL204834A0 (enExample)
WO (1) WO2009044170A1 (enExample)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
CA2730068A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
DE202009019021U1 (de) 2008-09-22 2015-09-24 University Of Washington Msp-nanoporen
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
BR112013020411B1 (pt) 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CA2861808C (en) 2011-12-29 2021-02-23 Oxford Nanopore Technologies Limited Enzyme method
EP3736339B1 (en) * 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
CA2879355C (en) 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN103267785B (zh) * 2013-05-14 2016-04-13 西北大学 一种检测药物包裹或释放过程的单分子分析方法
GB201316849D0 (en) 2013-09-23 2013-11-06 Isis Innovation Method
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015161117A2 (en) 2014-04-16 2015-10-22 The Uab Research Foundation Msp nanopores and uses thereof
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔，包含其的组合物及其应用
KR102222191B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
CN118272512A (zh) 2016-04-06 2024-07-02 牛津纳米孔科技公开有限公司 突变体孔
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3464616B1 (en) 2016-05-25 2022-05-04 Oxford Nanopore Technologies plc Method
CN106932448B (zh) * 2017-02-26 2019-06-21 西北大学 一种基于纳米孔单分子技术的手性分子识别和对映体过量检测方法
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
EP3645552B1 (en) 2017-06-30 2023-06-28 Vib Vzw Novel protein pores
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
IL293024B1 (en) * 2019-11-19 2026-07-01 Univ Groningen Artificial insemination, methods and uses
CN117229363A (zh) * 2022-06-06 2023-12-15 天津赛德生物制药有限公司 用于肺癌早期诊断的肽核酸功能化核孔膜及制备方法与应用
CN120435657A (zh) 2022-10-28 2025-08-05 格罗宁根大学 蛋白质的基于纳米孔的分析

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI82266C (fi) 1982-10-19 1991-02-11 Cetus Corp Foerfarande foer framstaellning av il-2 -mutein.
EP0753071A1 (en) * 1993-04-28 1997-01-15 Worcester Foundation For Experimental Biology Cell-targeted lytic pore-forming agents
US5777078A (en) * 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) * 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) * 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) * 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) * 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1192103A1 (en) * 1999-06-22 2002-04-03 President And Fellows of Harvard College Control of solid state dimensional features
CA2376650C (en) * 1999-06-29 2008-01-15 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
EP1238105A2 (en) * 1999-12-02 2002-09-11 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
US6916665B2 (en) * 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
US7001792B2 (en) * 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US7807408B2 (en) * 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
US6863833B1 (en) * 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
WO2003095669A1 (en) 2002-05-10 2003-11-20 The Texas A & M University System Stochastic sensing through covalent interactions
US7163658B2 (en) * 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2006020775A2 (en) 2004-08-13 2006-02-23 Harvard University An ultra high-throughput opti-nanopore dna readout platform
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) * 2005-11-15 2005-12-21 Isis Innovation Methods using pores
AU2006331512B2 (en) 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
US20100311602A1 (en) 2006-10-13 2010-12-09 J. Craig Venter Institute, Inc. Sequencing method
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
WO2008124107A1 (en) 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
JP2011515102A (ja) * 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 核酸シーケンシング用組成物及び方法
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CA2730068A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
DE202009019021U1 (de) 2008-09-22 2015-09-24 University Of Washington Msp-nanoporen

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