JP6749243B2 - １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 - Google Patents
１つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 Download PDFInfo
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- JP6749243B2 JP6749243B2 JP2016547868A JP2016547868A JP6749243B2 JP 6749243 B2 JP6749243 B2 JP 6749243B2 JP 2016547868 A JP2016547868 A JP 2016547868A JP 2016547868 A JP2016547868 A JP 2016547868A JP 6749243 B2 JP6749243 B2 JP 6749243B2
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- polynucleotide
- pore
- target polynucleotide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
- C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
- C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/GB2014/050175 WO2014135838A1 (en) | 2013-03-08 | 2014-01-22 | Enzyme stalling method |
| GBPCT/GB2014/050175 | 2014-01-22 | ||
| GBGB1406151.9A GB201406151D0 (en) | 2014-04-04 | 2014-04-04 | Method |
| GB1406155.0 | 2014-04-04 | ||
| GB1406151.9 | 2014-04-04 | ||
| GBGB1406155.0A GB201406155D0 (en) | 2014-04-04 | 2014-04-04 | Method |
| GB1416197.0 | 2014-09-12 | ||
| GB201416197A GB201416197D0 (en) | 2014-09-12 | 2014-09-12 | Method |
| PCT/GB2015/050140 WO2015110813A1 (en) | 2014-01-22 | 2015-01-22 | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017503517A JP2017503517A (ja) | 2017-02-02 |
| JP2017503517A5 JP2017503517A5 (enExample) | 2018-03-08 |
| JP6749243B2 true JP6749243B2 (ja) | 2020-09-02 |
Family
ID=53680879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016547868A Active JP6749243B2 (ja) | 2014-01-22 | 2015-01-22 | １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US10385389B2 (enExample) |
| EP (1) | EP3097210B2 (enExample) |
| JP (1) | JP6749243B2 (enExample) |
| CN (2) | CN111534504B (enExample) |
| AU (1) | AU2015208919B9 (enExample) |
| CA (1) | CA2937411C (enExample) |
| WO (1) | WO2015110813A1 (enExample) |
Families Citing this family (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2010209528B2 (en) | 2009-01-30 | 2015-10-01 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| US10808231B2 (en) | 2012-07-19 | 2020-10-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN117965707A (zh) | 2013-10-18 | 2024-05-03 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| AU2015208919B9 (en) | 2014-01-22 | 2021-04-01 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| WO2015166275A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| CN119899249A (zh) | 2014-09-01 | 2025-04-29 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| EP3283887B1 (en) | 2015-04-14 | 2021-07-21 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
| CN108779170A (zh) | 2016-03-02 | 2018-11-09 | 牛津纳米孔技术公司 | 突变孔 |
| AU2017246690B2 (en) | 2016-04-06 | 2022-03-24 | Oxford Nanopore Technologies Plc | Mutant pore |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| EP4063521B1 (en) | 2016-05-25 | 2024-02-21 | Oxford Nanopore Technologies PLC | Method of nanopore sequencing |
| GB201609220D0 (en) * | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB2559117B (en) | 2017-01-19 | 2019-11-27 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method, kit and system |
| AU2018218531B2 (en) * | 2017-02-10 | 2021-01-28 | Oxford Nanopore Technologies Limited | Modified nanopores, compositions comprising the same, and uses thereof |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CA3066715A1 (en) | 2017-06-30 | 2019-01-03 | Vib Vzw | Novel protein pores |
| GB2569977A (en) * | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB2574197B (en) | 2018-05-23 | 2022-01-05 | Oxford Nanopore Tech Ltd | Double stranded polynucleotide synthesis method and system. |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB201811811D0 (en) | 2018-07-19 | 2018-09-05 | Oxford Nanopore Tech Ltd | Method |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| JP6545888B1 (ja) * | 2018-11-09 | 2019-07-17 | 国立大学法人東北大学 | 情報処理システム、公開鍵の変更方法、およびプログラム |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| GB2602736A (en) * | 2019-09-18 | 2022-07-13 | Hitachi High Tech Corp | Adapter molecule, biomolecule-adapter molecule complex in which said adapter molecule and biomolecule are bound, biomolecule analysis apparatus, and |
| GB2603061A (en) * | 2019-09-18 | 2022-07-27 | Hitachi High Tech Corp | Adapter molecule, biomolecule-adapter molecule complex composed of adapter molecule and biomolecule bound together, biomolecule analyzer and biomolecule |
| WO2021056598A1 (zh) | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mmup单体变体及其应用 |
| WO2021056599A1 (zh) * | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| WO2021255475A1 (en) * | 2020-06-18 | 2021-12-23 | Oxford Nanopore Technologies Limited | A method of selectively characterising a polynucleotide using a detector |
| WO2021255476A2 (en) | 2020-06-18 | 2021-12-23 | Oxford Nanopore Technologies Limited | Method |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| US20220059187A1 (en) | 2020-08-07 | 2022-02-24 | Oxford Nanopore Technologies Limited | Methods of detecting nucleic acid barcodes |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| CN112851765B (zh) * | 2021-02-09 | 2023-02-28 | 中国科学院武汉病毒研究所 | 蛋白或肽与核酸共价连接的方法 |
| CN112851771A (zh) * | 2021-02-09 | 2021-05-28 | 中国科学院武汉病毒研究所 | 连接核酸与蛋白或肽的方法 |
| WO2022243692A1 (en) | 2021-05-19 | 2022-11-24 | Oxford Nanopore Technologies Plc | Methods for complement strand sequencing |
| CN114134142B (zh) * | 2021-11-23 | 2024-05-28 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN113862264A (zh) * | 2021-12-03 | 2021-12-31 | 北京齐碳科技有限公司 | 用于靶多核苷酸测序的衔接子、构建体、方法和应用 |
| CN114262735B (zh) * | 2021-12-15 | 2025-09-16 | 成都齐碳科技有限公司 | 用于表征多核苷酸的衔接体及其用途 |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202204919D0 (en) | 2022-04-04 | 2022-05-18 | Oxford Nanopore Tech Plc | Method |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| CN114854826A (zh) * | 2022-05-13 | 2022-08-05 | 北京齐碳科技有限公司 | 序列、包含序列的接头及其用途 |
| WO2023222657A1 (en) | 2022-05-17 | 2023-11-23 | Oxford Nanopore Technologies Plc | Method and adaptors |
| US20250304947A1 (en) | 2022-05-18 | 2025-10-02 | Oxford Nanopore Technologies plc. | Compositions and methods for nucleic acid extraction and purification |
| GB202207267D0 (en) | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
| CN114836452B (zh) * | 2022-05-19 | 2023-12-05 | 浙江安诺优达生物科技有限公司 | 一种密码子优化的核酸外切酶ⅲ基因及其表达方法 |
| CN116478983B (zh) * | 2022-11-21 | 2023-10-24 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| WO2024138320A1 (zh) * | 2022-12-26 | 2024-07-04 | 深圳华大生命科学研究院 | 减少测序文库中游离接头的方法 |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
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| GB202407247D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
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| EP3097210B1 (en) | 2018-11-14 |
| US20200087724A1 (en) | 2020-03-19 |
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| CN111534504A (zh) | 2020-08-14 |
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| AU2015208919B2 (en) | 2021-03-11 |
| CN106103741A (zh) | 2016-11-09 |
| CA2937411A1 (en) | 2015-07-30 |
| US11725235B2 (en) | 2023-08-15 |
| AU2015208919A1 (en) | 2016-07-21 |
| CA2937411C (en) | 2023-09-26 |
| AU2015208919B9 (en) | 2021-04-01 |
| US10385389B2 (en) | 2019-08-20 |
| EP3097210A1 (en) | 2016-11-30 |
| US20240026441A1 (en) | 2024-01-25 |
| CN106103741B (zh) | 2020-03-13 |
| CN111534504B (zh) | 2024-06-21 |
| US20170107569A1 (en) | 2017-04-20 |
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