JP2015503917A5 - - Google Patents
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- JP2015503917A5 JP2015503917A5 JP2014549533A JP2014549533A JP2015503917A5 JP 2015503917 A5 JP2015503917 A5 JP 2015503917A5 JP 2014549533 A JP2014549533 A JP 2014549533A JP 2014549533 A JP2014549533 A JP 2014549533A JP 2015503917 A5 JP2015503917 A5 JP 2015503917A5
- Authority
- JP
- Japan
- Prior art keywords
- seq
- target polynucleotide
- helicase
- amino acid
- pore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108060004795 Methyltransferase Proteins 0.000 claims 38
- 102000040430 polynucleotide Human genes 0.000 claims 35
- 108091033319 polynucleotide Proteins 0.000 claims 35
- 239000002157 polynucleotide Substances 0.000 claims 35
- 238000000034 method Methods 0.000 claims 23
- 239000011148 porous material Substances 0.000 claims 19
- 150000001413 amino acids Chemical class 0.000 claims 14
- 238000005259 measurement Methods 0.000 claims 11
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- 230000000694 effects Effects 0.000 claims 5
- 150000003839 salts Chemical class 0.000 claims 4
- 108091005703 transmembrane proteins Proteins 0.000 claims 3
- 102000035160 transmembrane proteins Human genes 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 101710203388 Outer membrane porin G Proteins 0.000 claims 2
- 108010013381 Porins Proteins 0.000 claims 2
- 238000012512 characterization method Methods 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 230000003287 optical effect Effects 0.000 claims 2
- 102000007739 porin activity proteins Human genes 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 125000006850 spacer group Chemical group 0.000 claims 2
- 101710092462 Alpha-hemolysin Proteins 0.000 claims 1
- 108010006464 Hemolysin Proteins Proteins 0.000 claims 1
- 108010014603 Leukocidins Proteins 0.000 claims 1
- 102000004895 Lipoproteins Human genes 0.000 claims 1
- 108090001030 Lipoproteins Proteins 0.000 claims 1
- 241000187480 Mycobacterium smegmatis Species 0.000 claims 1
- 241000588653 Neisseria Species 0.000 claims 1
- 108010073429 Type V Secretion Systems Proteins 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- OGQYPPBGSLZBEG-UHFFFAOYSA-N dimethyl(dioctadecyl)azanium Chemical compound CCCCCCCCCCCCCCCCCC[N+](C)(C)CCCCCCCCCCCCCCCCCC OGQYPPBGSLZBEG-UHFFFAOYSA-N 0.000 claims 1
- 230000005669 field effect Effects 0.000 claims 1
- 239000003228 hemolysin Substances 0.000 claims 1
- 238000002847 impedance measurement Methods 0.000 claims 1
- 239000002346 layers by function Substances 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 108010014203 outer membrane phospholipase A Proteins 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161581332P | 2011-12-29 | 2011-12-29 | |
| US61/581,332 | 2011-12-29 | ||
| PCT/GB2012/053274 WO2013098562A2 (en) | 2011-12-29 | 2012-12-28 | Enzyme method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015503917A JP2015503917A (ja) | 2015-02-05 |
| JP2015503917A5 true JP2015503917A5 (enExample) | 2016-02-18 |
| JP6228128B2 JP6228128B2 (ja) | 2017-11-08 |
Family
ID=47561658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014549533A Active JP6228128B2 (ja) | 2011-12-29 | 2012-12-28 | 酵素法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10385382B2 (enExample) |
| EP (1) | EP2798084B1 (enExample) |
| JP (1) | JP6228128B2 (enExample) |
| KR (1) | KR102086182B1 (enExample) |
| CN (1) | CN104126018B (enExample) |
| AU (1) | AU2012360244B2 (enExample) |
| BR (1) | BR112014016112B8 (enExample) |
| CA (1) | CA2861808C (enExample) |
| WO (1) | WO2013098562A2 (enExample) |
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| EP3029467B1 (en) | 2008-09-22 | 2020-01-08 | University of Washington | Msp nanopores and related methods |
| JP5843614B2 (ja) | 2009-01-30 | 2016-01-13 | オックスフォード ナノポア テクノロジーズ リミテッド | 膜貫通配列決定における核酸構築物のためのアダプター |
| AU2012264497B2 (en) | 2011-05-27 | 2017-06-15 | Oxford Nanopore Technologies Limited | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| WO2013041878A1 (en) | 2011-09-23 | 2013-03-28 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| JP6226869B2 (ja) | 2011-10-21 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素法 |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| KR102086182B1 (ko) | 2011-12-29 | 2020-03-06 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 방법 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
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| EP3736339B1 (en) | 2012-02-16 | 2022-07-27 | Oxford Nanopore Technologies plc | Analysis of measurements of a polymer |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
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| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| US9995728B2 (en) | 2012-11-06 | 2018-06-12 | Oxford Nanopore Technologies Ltd. | Quadruplex method |
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| GB201314695D0 (en) * | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
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| CN103265958A (zh) * | 2013-06-08 | 2013-08-28 | 东莞市保得生物工程有限公司 | 一种商品化有机物料腐熟剂的制备方法 |
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| AU2018218531B2 (en) | 2017-02-10 | 2021-01-28 | Oxford Nanopore Technologies Limited | Modified nanopores, compositions comprising the same, and uses thereof |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| KR102593484B1 (ko) | 2017-06-29 | 2023-10-24 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 나노포어를 통한 폴리머의 결정적 스텝핑 |
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2012
- 2012-12-28 KR KR1020147020837A patent/KR102086182B1/ko active Active
- 2012-12-28 CA CA2861808A patent/CA2861808C/en active Active
- 2012-12-28 WO PCT/GB2012/053274 patent/WO2013098562A2/en not_active Ceased
- 2012-12-28 BR BR112014016112A patent/BR112014016112B8/pt active IP Right Grant
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