JP2016537967A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2016537967A5 JP2016537967A5 JP2016521740A JP2016521740A JP2016537967A5 JP 2016537967 A5 JP2016537967 A5 JP 2016537967A5 JP 2016521740 A JP2016521740 A JP 2016521740A JP 2016521740 A JP2016521740 A JP 2016521740A JP 2016537967 A5 JP2016537967 A5 JP 2016537967A5
- Authority
- JP
- Japan
- Prior art keywords
- isolated
- wnt
- prscs
- stem cells
- item
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 149
- 239000003112 inhibitor Substances 0.000 claims description 99
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims description 69
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims description 69
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims description 69
- 102000013814 Wnt Human genes 0.000 claims description 65
- 108050003627 Wnt Proteins 0.000 claims description 65
- 230000011664 signaling Effects 0.000 claims description 63
- 210000000130 stem cell Anatomy 0.000 claims description 48
- 230000002207 retinal effect Effects 0.000 claims description 44
- WRKPZSMRWPJJDH-UHFFFAOYSA-N N-(6-methyl-1,3-benzothiazol-2-yl)-2-[(4-oxo-3-phenyl-6,7-dihydrothieno[3,2-d]pyrimidin-2-yl)thio]acetamide Chemical compound S1C2=CC(C)=CC=C2N=C1NC(=O)CSC1=NC=2CCSC=2C(=O)N1C1=CC=CC=C1 WRKPZSMRWPJJDH-UHFFFAOYSA-N 0.000 claims description 43
- 150000003384 small molecules Chemical class 0.000 claims description 41
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 39
- 241000282414 Homo sapiens Species 0.000 claims description 38
- FHYUGAJXYORMHI-UHFFFAOYSA-N SB 431542 Chemical compound C1=CC(C(=O)N)=CC=C1C1=NC(C=2C=C3OCOC3=CC=2)=C(C=2N=CC=CC=2)N1 FHYUGAJXYORMHI-UHFFFAOYSA-N 0.000 claims description 36
- 210000004027 cell Anatomy 0.000 claims description 36
- 201000007737 Retinal degeneration Diseases 0.000 claims description 34
- 230000004258 retinal degeneration Effects 0.000 claims description 34
- 238000012258 culturing Methods 0.000 claims description 30
- 210000000844 retinal pigment epithelial cell Anatomy 0.000 claims description 28
- CDOVNWNANFFLFJ-UHFFFAOYSA-N 4-[6-[4-(1-piperazinyl)phenyl]-3-pyrazolo[1,5-a]pyrimidinyl]quinoline Chemical compound C1CNCCN1C1=CC=C(C2=CN3N=CC(=C3N=C2)C=2C3=CC=CC=C3N=CC=2)C=C1 CDOVNWNANFFLFJ-UHFFFAOYSA-N 0.000 claims description 27
- 210000000608 photoreceptor cell Anatomy 0.000 claims description 25
- 210000003994 retinal ganglion cell Anatomy 0.000 claims description 25
- 239000001963 growth medium Substances 0.000 claims description 23
- 210000003583 retinal pigment epithelium Anatomy 0.000 claims description 22
- 239000007787 solid Substances 0.000 claims description 22
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 102000001267 GSK3 Human genes 0.000 claims description 20
- 108010014905 Glycogen Synthase Kinase 3 Proteins 0.000 claims description 20
- -1 J01-017a Proteins 0.000 claims description 20
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 20
- 241000124008 Mammalia Species 0.000 claims description 19
- 239000003636 conditioned culture medium Substances 0.000 claims description 19
- 102100034134 Activin receptor type-1B Human genes 0.000 claims description 18
- 101710173011 Activin receptor type-1B Proteins 0.000 claims description 18
- 108010070047 Notch Receptors Proteins 0.000 claims description 18
- 108010052946 Activin Receptors Proteins 0.000 claims description 16
- 102000018918 Activin Receptors Human genes 0.000 claims description 16
- 238000004519 manufacturing process Methods 0.000 claims description 16
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 14
- 102000045246 noggin Human genes 0.000 claims description 13
- 108700007229 noggin Proteins 0.000 claims description 13
- 210000002469 basement membrane Anatomy 0.000 claims description 11
- 108010082117 matrigel Proteins 0.000 claims description 11
- 230000007781 signaling event Effects 0.000 claims description 11
- 239000000126 substance Substances 0.000 claims description 11
- 102100030074 Dickkopf-related protein 1 Human genes 0.000 claims description 10
- 101710099518 Dickkopf-related protein 1 Proteins 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 10
- 210000002966 serum Anatomy 0.000 claims description 10
- 239000012190 activator Substances 0.000 claims description 9
- 239000003102 growth factor Substances 0.000 claims description 9
- 230000019491 signal transduction Effects 0.000 claims description 9
- 102100025423 Bone morphogenetic protein receptor type-1A Human genes 0.000 claims description 8
- 101710120270 Bone morphogenetic protein receptor type-1A Proteins 0.000 claims description 8
- 101000799140 Homo sapiens Activin receptor type-1 Proteins 0.000 claims description 8
- 108091008605 VEGF receptors Proteins 0.000 claims description 8
- 102100033177 Vascular endothelial growth factor receptor 2 Human genes 0.000 claims description 8
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 claims description 8
- GPTFURBXHJWNHR-UHFFFAOYSA-N protopine Chemical compound C1=C2C(=O)CC3=CC=C4OCOC4=C3CN(C)CCC2=CC2=C1OCO2 GPTFURBXHJWNHR-UHFFFAOYSA-N 0.000 claims description 8
- 230000002195 synergetic effect Effects 0.000 claims description 8
- 102000005650 Notch Receptors Human genes 0.000 claims description 7
- 230000005764 inhibitory process Effects 0.000 claims description 7
- 229960003080 taurine Drugs 0.000 claims description 7
- KXDAEFPNCMNJSK-UHFFFAOYSA-N Benzamide Chemical compound NC(=O)C1=CC=CC=C1 KXDAEFPNCMNJSK-UHFFFAOYSA-N 0.000 claims description 6
- DWJXYEABWRJFSP-XOBRGWDASA-N DAPT Chemical compound N([C@@H](C)C(=O)N[C@H](C(=O)OC(C)(C)C)C=1C=CC=CC=1)C(=O)CC1=CC(F)=CC(F)=C1 DWJXYEABWRJFSP-XOBRGWDASA-N 0.000 claims description 6
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 6
- 239000006143 cell culture medium Substances 0.000 claims description 6
- 238000000338 in vitro Methods 0.000 claims description 6
- 230000009459 hedgehog signaling Effects 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 5
- 230000001537 neural effect Effects 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- BERLXWPRSBJFHO-UHFFFAOYSA-N 2-(5-chloro-2-fluorophenyl)-n-pyridin-4-ylpteridin-4-amine Chemical compound FC1=CC=C(Cl)C=C1C1=NC(NC=2C=CN=CC=2)=C(N=CC=N2)C2=N1 BERLXWPRSBJFHO-UHFFFAOYSA-N 0.000 claims description 4
- HIJMSZGHKQPPJS-UHFFFAOYSA-N 3-(6-methylpyridin-2-yl)-n-phenyl-4-quinolin-4-ylpyrazole-1-carbothioamide Chemical compound CC1=CC=CC(C=2C(=CN(N=2)C(=S)NC=2C=CC=CC=2)C=2C3=CC=CC=C3N=CC=2)=N1 HIJMSZGHKQPPJS-UHFFFAOYSA-N 0.000 claims description 4
- IPICFSFUAKDZEI-UHFFFAOYSA-N 3-methyl-2-[2-[[3-methyl-2-[2-[(2-methylpropan-2-yl)oxycarbonylamino]propanoyloxy]butanoyl]amino]propanoyloxy]butanoic acid Chemical compound CC(C)C(C(O)=O)OC(=O)C(C)NC(=O)C(C(C)C)OC(=O)C(C)NC(=O)OC(C)(C)C IPICFSFUAKDZEI-UHFFFAOYSA-N 0.000 claims description 4
- DKPQHFZUICCZHF-UHFFFAOYSA-N 6-[2-tert-butyl-5-(6-methyl-2-pyridinyl)-1H-imidazol-4-yl]quinoxaline Chemical compound CC1=CC=CC(C2=C(N=C(N2)C(C)(C)C)C=2C=C3N=CC=NC3=CC=2)=N1 DKPQHFZUICCZHF-UHFFFAOYSA-N 0.000 claims description 4
- AQGNHMOJWBZFQQ-UHFFFAOYSA-N CT 99021 Chemical compound CC1=CNC(C=2C(=NC(NCCNC=3N=CC(=CC=3)C#N)=NC=2)C=2C(=CC(Cl)=CC=2)Cl)=N1 AQGNHMOJWBZFQQ-UHFFFAOYSA-N 0.000 claims description 4
- 102100030634 Homeobox protein OTX2 Human genes 0.000 claims description 4
- 102100027345 Homeobox protein SIX3 Human genes 0.000 claims description 4
- 101000584400 Homo sapiens Homeobox protein OTX2 Proteins 0.000 claims description 4
- 101000651928 Homo sapiens Homeobox protein SIX3 Proteins 0.000 claims description 4
- 101000835956 Homo sapiens Homeobox protein SIX6 Proteins 0.000 claims description 4
- 101001020544 Homo sapiens LIM/homeobox protein Lhx2 Proteins 0.000 claims description 4
- 102100029408 Interferon-inducible double-stranded RNA-dependent protein kinase activator A Human genes 0.000 claims description 4
- 101710154084 Interferon-inducible double-stranded RNA-dependent protein kinase activator A Proteins 0.000 claims description 4
- 102100036132 LIM/homeobox protein Lhx2 Human genes 0.000 claims description 4
- IBCXZJCWDGCXQT-UHFFFAOYSA-N LY 364947 Chemical compound C=1C=NC2=CC=CC=C2C=1C1=CNN=C1C1=CC=CC=N1 IBCXZJCWDGCXQT-UHFFFAOYSA-N 0.000 claims description 4
- 108010072083 NSC 668036 Proteins 0.000 claims description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 claims description 4
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 4
- 238000011977 dual antiplatelet therapy Methods 0.000 claims description 4
- AVOLMBLBETYQHX-UHFFFAOYSA-N etacrynic acid Chemical compound CCC(=C)C(=O)C1=CC=C(OCC(O)=O)C(Cl)=C1Cl AVOLMBLBETYQHX-UHFFFAOYSA-N 0.000 claims description 4
- 229960000890 hydrocortisone Drugs 0.000 claims description 4
- 239000012913 medium supplement Substances 0.000 claims description 4
- FYBHCRQFSFYWPY-UHFFFAOYSA-N purmorphamine Chemical compound C1CCCCC1N1C2=NC(OC=3C4=CC=CC=C4C=CC=3)=NC(NC=3C=CC(=CC=3)N3CCOCC3)=C2N=C1 FYBHCRQFSFYWPY-UHFFFAOYSA-N 0.000 claims description 4
- SLGRAIAQIAUZAQ-UHFFFAOYSA-N toxoflavin Chemical compound CN1N=CN=C2C1=NC(=O)N(C)C2=O SLGRAIAQIAUZAQ-UHFFFAOYSA-N 0.000 claims description 4
- 229940035722 triiodothyronine Drugs 0.000 claims description 4
- DPDZHVCKYBCJHW-UHFFFAOYSA-N 4-[4-(2,3-dihydro-1,4-benzodioxin-6-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]benzamide Chemical compound C1=CC(C(=O)N)=CC=C1C1=NC(C=2C=C3OCCOC3=CC=2)=C(C=2N=CC=CC=2)N1 DPDZHVCKYBCJHW-UHFFFAOYSA-N 0.000 claims description 3
- 102100034111 Activin receptor type-1 Human genes 0.000 claims description 3
- 241000283690 Bos taurus Species 0.000 claims description 3
- 241000283707 Capra Species 0.000 claims description 3
- 241000700199 Cavia porcellus Species 0.000 claims description 3
- 241000282693 Cercopithecidae Species 0.000 claims description 3
- 241000283073 Equus caballus Species 0.000 claims description 3
- 241000289669 Erinaceus europaeus Species 0.000 claims description 3
- 241000282326 Felis catus Species 0.000 claims description 3
- 102100025448 Homeobox protein SIX6 Human genes 0.000 claims description 3
- 101000687905 Homo sapiens Transcription factor SOX-2 Proteins 0.000 claims description 3
- 239000005517 L01XE01 - Imatinib Substances 0.000 claims description 3
- 239000002145 L01XE14 - Bosutinib Substances 0.000 claims description 3
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 3
- 241000772415 Neovison vison Species 0.000 claims description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 3
- 108010032788 PAX6 Transcription Factor Proteins 0.000 claims description 3
- 241001494479 Pecora Species 0.000 claims description 3
- 241000009328 Perro Species 0.000 claims description 3
- 241000700159 Rattus Species 0.000 claims description 3
- 108010017324 STAT3 Transcription Factor Proteins 0.000 claims description 3
- 241000282898 Sus scrofa Species 0.000 claims description 3
- 102100024270 Transcription factor SOX-2 Human genes 0.000 claims description 3
- 108010051765 Type I Bone Morphogenetic Protein Receptors Proteins 0.000 claims description 3
- 102000019044 Type I Bone Morphogenetic Protein Receptors Human genes 0.000 claims description 3
- 241000282458 Ursus sp. Species 0.000 claims description 3
- 108010023082 activin A Proteins 0.000 claims description 3
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 3
- 229960003736 bosutinib Drugs 0.000 claims description 3
- UBPYILGKFZZVDX-UHFFFAOYSA-N bosutinib Chemical compound C1=C(Cl)C(OC)=CC(NC=2C3=CC(OC)=C(OCCCN4CCN(C)CC4)C=C3N=CC=2C#N)=C1Cl UBPYILGKFZZVDX-UHFFFAOYSA-N 0.000 claims description 3
- SRJYZPCBWDVSGO-UHFFFAOYSA-N cladochrome E Natural products COC1=CC(O)=C(C(C(OC)=C(CC(C)OC(=O)OC=2C=CC(O)=CC=2)C2=3)=O)C2=C1C1=C(OC)C=C(O)C(C(C=2OC)=O)=C1C=3C=2CC(C)OC(=O)C1=CC=CC=C1 SRJYZPCBWDVSGO-UHFFFAOYSA-N 0.000 claims description 3
- 229960002411 imatinib Drugs 0.000 claims description 3
- KTUFNOKKBVMGRW-UHFFFAOYSA-N imatinib Chemical compound C1CN(C)CCN1CC1=CC=C(C(=O)NC=2C=C(NC=3N=C(C=CN=3)C=3C=NC=CC=3)C(C)=CC=2)C=C1 KTUFNOKKBVMGRW-UHFFFAOYSA-N 0.000 claims description 3
- 230000004660 morphological change Effects 0.000 claims description 3
- 210000005036 nerve Anatomy 0.000 claims description 3
- 229960003966 nicotinamide Drugs 0.000 claims description 3
- 235000005152 nicotinamide Nutrition 0.000 claims description 3
- 239000011570 nicotinamide Substances 0.000 claims description 3
- 229930002330 retinoic acid Natural products 0.000 claims description 3
- 229960001727 tretinoin Drugs 0.000 claims description 3
- DVKQVRZMKBDMDH-FULWYAMNSA-N (4as,6r,7r,7ar)-6-(6-amino-8-bromopurin-9-yl)-2-hydroxy-2-oxo-4a,6,7,7a-tetrahydro-4h-furo[3,2-d][1,3,2]dioxaphosphinin-7-ol Chemical compound C([C@@H]1O2)OP(O)(=O)O[C@@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1Br DVKQVRZMKBDMDH-FULWYAMNSA-N 0.000 claims description 2
- HQWTUOLCGKIECB-XZWHSSHBSA-N (6S,9aS)-6-[(4-hydroxyphenyl)methyl]-8-(1-naphthalenylmethyl)-4,7-dioxo-N-(phenylmethyl)-3,6,9,9a-tetrahydro-2H-pyrazino[1,2-a]pyrimidine-1-carboxamide Chemical compound C1=CC(O)=CC=C1C[C@H]1C(=O)N(CC=2C3=CC=CC=C3C=CC=2)C[C@H]2N1C(=O)CCN2C(=O)NCC1=CC=CC=C1 HQWTUOLCGKIECB-XZWHSSHBSA-N 0.000 claims description 2
- LBPKYPYHDKKRFS-UHFFFAOYSA-N 1,5-naphthyridine, 2-[3-(6-methyl-2-pyridinyl)-1h-pyrazol-4-yl]- Chemical compound CC1=CC=CC(C2=C(C=NN2)C=2N=C3C=CC=NC3=CC=2)=N1 LBPKYPYHDKKRFS-UHFFFAOYSA-N 0.000 claims description 2
- KHZOJCQBHJUJFY-UHFFFAOYSA-N 2-[4-(2-methylpyridin-4-yl)phenyl]-n-(4-pyridin-3-ylphenyl)acetamide Chemical compound C1=NC(C)=CC(C=2C=CC(CC(=O)NC=3C=CC(=CC=3)C=3C=NC=CC=3)=CC=2)=C1 KHZOJCQBHJUJFY-UHFFFAOYSA-N 0.000 claims description 2
- JBJYTZXCZDNOJW-UHFFFAOYSA-N 3-[(2,4,6-trimethoxyphenyl)methylidene]-1H-indol-2-one Chemical compound COC1=CC(OC)=CC(OC)=C1C=C1C2=CC=CC=C2NC1=O JBJYTZXCZDNOJW-UHFFFAOYSA-N 0.000 claims description 2
- YJQYHFMKGAVKDP-UHFFFAOYSA-N 3-butanoyl-1,8-dihydroxy-2-methylphenanthrene-9,10-dione Chemical compound C12=CC=CC(O)=C2C(=O)C(=O)C2=C1C=C(C(=O)CCC)C(C)=C2O YJQYHFMKGAVKDP-UHFFFAOYSA-N 0.000 claims description 2
- 125000001255 4-fluorophenyl group Chemical group [H]C1=C([H])C(*)=C([H])C([H])=C1F 0.000 claims description 2
- RVNSQVIUFZVNAU-UHFFFAOYSA-N 5-[(2-hydroxynaphthalen-1-yl)methyl]-6-phenyl-2-sulfanylidene-1h-pyrimidin-4-one Chemical compound OC1=CC=C2C=CC=CC2=C1CC(C(NC(=S)N1)=O)=C1C1=CC=CC=C1 RVNSQVIUFZVNAU-UHFFFAOYSA-N 0.000 claims description 2
- KRIKILRRJCIWNB-UHFFFAOYSA-N 5-[[4-(4-methoxyphenyl)-5-pyridin-4-yl-1,2,4-triazol-3-yl]sulfanylmethyl]-3-(4-methylphenyl)-1,2,4-oxadiazole Chemical compound C1=CC(OC)=CC=C1N1C(C=2C=CN=CC=2)=NN=C1SCC1=NC(C=2C=CC(C)=CC=2)=NO1 KRIKILRRJCIWNB-UHFFFAOYSA-N 0.000 claims description 2
- 102000017944 Dishevelled Human genes 0.000 claims description 2
- 108050007016 Dishevelled Proteins 0.000 claims description 2
- 229930183931 Filipin Natural products 0.000 claims description 2
- 101000685824 Homo sapiens Probable RNA polymerase II nuclear localization protein SLC7A6OS Proteins 0.000 claims description 2
- XXYGTCZJJLTAGH-UHFFFAOYSA-N LGK974 Chemical compound C1=NC(C)=CC(C=2C(=CC(CC(=O)NC=3N=CC(=CC=3)C=3N=CC=NC=3)=CN=2)C)=C1 XXYGTCZJJLTAGH-UHFFFAOYSA-N 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 claims description 2
- BYDVKOWNFLHTJG-UHFFFAOYSA-N N-[(4-oxo-3-phenyl-6,7-dihydrothieno[3,2-d]pyrimidin-2-yl)sulfanyl]acetamide Chemical compound C(C)(=O)NSC=1N(C(C2=C(N=1)CCS2)=O)C1=CC=CC=C1 BYDVKOWNFLHTJG-UHFFFAOYSA-N 0.000 claims description 2
- DXCUKNQANPLTEJ-UHFFFAOYSA-N PD173074 Chemical compound CC(C)(C)NC(=O)NC1=NC2=NC(NCCCCN(CC)CC)=NC=C2C=C1C1=CC(OC)=CC(OC)=C1 DXCUKNQANPLTEJ-UHFFFAOYSA-N 0.000 claims description 2
- PSNKGVAXBSAHCH-UHFFFAOYSA-N PF-670462 Chemical compound Cl.Cl.NC1=NC=CC(C=2N(C=NC=2C=2C=CC(F)=CC=2)C2CCCCC2)=N1 PSNKGVAXBSAHCH-UHFFFAOYSA-N 0.000 claims description 2
- OYONTEXKYJZFHA-SSHUPFPWSA-N PHA-665752 Chemical compound CC=1C(C(=O)N2[C@H](CCC2)CN2CCCC2)=C(C)NC=1\C=C(C1=C2)/C(=O)NC1=CC=C2S(=O)(=O)CC1=C(Cl)C=CC=C1Cl OYONTEXKYJZFHA-SSHUPFPWSA-N 0.000 claims description 2
- UYJZZVDLGDDTCL-UHFFFAOYSA-N PJ34 Chemical compound C1=CC=C2C3=CC(NC(=O)CN(C)C)=CC=C3NC(=O)C2=C1 UYJZZVDLGDDTCL-UHFFFAOYSA-N 0.000 claims description 2
- 102100023136 Probable RNA polymerase II nuclear localization protein SLC7A6OS Human genes 0.000 claims description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 claims description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 claims description 2
- CDMGBJANTYXAIV-UHFFFAOYSA-N SB 203580 Chemical compound C1=CC(S(=O)C)=CC=C1C1=NC(C=2C=CC(F)=CC=2)=C(C=2C=CN=CC=2)N1 CDMGBJANTYXAIV-UHFFFAOYSA-N 0.000 claims description 2
- ZQUSFAUAYSEREK-WKILWMFISA-N SB-239063 Chemical compound COC1=NC=CC(C=2N(C=NC=2C=2C=CC(F)=CC=2)[C@@H]2CC[C@@H](O)CC2)=N1 ZQUSFAUAYSEREK-WKILWMFISA-N 0.000 claims description 2
- RFCWHQNNCOJYTR-IRCAEPKSSA-N Tautomycin Chemical compound O([C@H]([C@H](CC1)C)[C@H](C)CC[C@H](O)[C@H](C)C(=O)C[C@@H](O)[C@@H](OC)[C@H](OC(=O)C[C@@H](O)C=2C(OC(=O)C=2C)=O)C(C)C)[C@@]21CC[C@@H](C)[C@H](CC[C@H](C)C(C)=O)O2 RFCWHQNNCOJYTR-IRCAEPKSSA-N 0.000 claims description 2
- RFCWHQNNCOJYTR-UHFFFAOYSA-N Tautomycin Natural products CC=1C(=O)OC(=O)C=1C(O)CC(=O)OC(C(C)C)C(OC)C(O)CC(=O)C(C)C(O)CCC(C)C(C(CC1)C)OC21CCC(C)C(CCC(C)C(C)=O)O2 RFCWHQNNCOJYTR-UHFFFAOYSA-N 0.000 claims description 2
- KLGQSVMIPOVQAX-UHFFFAOYSA-N XAV939 Chemical compound N=1C=2CCSCC=2C(O)=NC=1C1=CC=C(C(F)(F)F)C=C1 KLGQSVMIPOVQAX-UHFFFAOYSA-N 0.000 claims description 2
- NDQDTBCXPOIQGT-UHNJQBFTSA-N [(2R,3R,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl-ADP Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)OC[C@H]1NC[C@H](O)[C@@H]1O NDQDTBCXPOIQGT-UHNJQBFTSA-N 0.000 claims description 2
- 229930193106 apicularen Natural products 0.000 claims description 2
- 229930192649 bafilomycin Natural products 0.000 claims description 2
- XDHNQDDQEHDUTM-UHFFFAOYSA-N bafliomycin A1 Natural products COC1C=CC=C(C)CC(C)C(O)C(C)C=C(C)C=C(OC)C(=O)OC1C(C)C(O)C(C)C1(O)OC(C(C)C)C(C)C(O)C1 XDHNQDDQEHDUTM-UHFFFAOYSA-N 0.000 claims description 2
- JWFLIMIGORGZMQ-UHFFFAOYSA-N cercosporin Natural products COC1=C(CC(C)O)c2c3c(CC(C)O)c(OC)c(O)c4C(=O)C=C5OCOc6cc(O)c(C1=O)c2c6c5c34 JWFLIMIGORGZMQ-UHFFFAOYSA-N 0.000 claims description 2
- 210000004748 cultured cell Anatomy 0.000 claims description 2
- 229960003199 etacrynic acid Drugs 0.000 claims description 2
- IMQSIXYSKPIGPD-NKYUYKLDSA-N filipin Chemical compound CCCCC[C@H](O)[C@@H]1[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@H](O)\C(C)=C\C=C\C=C\C=C\C=C\[C@H](O)[C@@H](C)OC1=O IMQSIXYSKPIGPD-NKYUYKLDSA-N 0.000 claims description 2
- 229950000152 filipin Drugs 0.000 claims description 2
- IMQSIXYSKPIGPD-UHFFFAOYSA-N filipin III Natural products CCCCCC(O)C1C(O)CC(O)CC(O)CC(O)CC(O)CC(O)CC(O)C(C)=CC=CC=CC=CC=CC(O)C(C)OC1=O IMQSIXYSKPIGPD-UHFFFAOYSA-N 0.000 claims description 2
- ZGSXEXBYLJIOGF-BOPNQXPFSA-N iwr-1 Chemical compound C=1C=CC2=CC=CN=C2C=1NC(=O)C(C=C1)=CC=C1N1C(=O)[C@@H]2C(C=C3)CC3[C@@H]2C1=O ZGSXEXBYLJIOGF-BOPNQXPFSA-N 0.000 claims description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 claims description 2
- QAXIGMXDHAMYPV-UHFFFAOYSA-N methyl 1,6,8,14a-tetrahydroxy-11-[(4-hydroxy-3,5-dimethoxy-6-methyloxan-2-yl)amino]-6a-methoxy-3-methyl-7,9,12,14-tetraoxo-5,6-dihydrobenzo[a]tetracene-2-carboxylate Chemical compound COC1C(O)C(OC)C(C)OC1NC(C(C1=C2)=O)=CC(=O)C1=C(O)C1=C2C(=O)C2(O)C3=C(O)C(C(=O)OC)=C(C)C=C3CC(O)C2(OC)C1=O QAXIGMXDHAMYPV-UHFFFAOYSA-N 0.000 claims description 2
- JJEDWBQZCRESJL-DEDYPNTBSA-N n-[(e)-(5-methylfuran-2-yl)methylideneamino]-2-phenoxybenzamide Chemical compound O1C(C)=CC=C1\C=N\NC(=O)C1=CC=CC=C1OC1=CC=CC=C1 JJEDWBQZCRESJL-DEDYPNTBSA-N 0.000 claims description 2
- RJMUSRYZPJIFPJ-UHFFFAOYSA-N niclosamide Chemical compound OC1=CC=C(Cl)C=C1C(=O)NC1=CC=C([N+]([O-])=O)C=C1Cl RJMUSRYZPJIFPJ-UHFFFAOYSA-N 0.000 claims description 2
- 229960001920 niclosamide Drugs 0.000 claims description 2
- QNDVLZJODHBUFM-WFXQOWMNSA-N okadaic acid Chemical compound C([C@H](O1)[C@H](C)/C=C/[C@H]2CC[C@@]3(CC[C@H]4O[C@@H](C([C@@H](O)[C@@H]4O3)=C)[C@@H](O)C[C@H](C)[C@@H]3[C@@H](CC[C@@]4(OCCCC4)O3)C)O2)C(C)=C[C@]21O[C@H](C[C@@](C)(O)C(O)=O)CC[C@H]2O QNDVLZJODHBUFM-WFXQOWMNSA-N 0.000 claims description 2
- VEFJHAYOIAAXEU-UHFFFAOYSA-N okadaic acid Natural products CC(CC(O)C1OC2CCC3(CCC(O3)C=CC(C)C4CC(=CC5(OC(CC(C)(O)C(=O)O)CCC5O)O4)C)OC2C(O)C1C)C6OC7(CCCCO7)CCC6C VEFJHAYOIAAXEU-UHFFFAOYSA-N 0.000 claims description 2
- LHNIIDJUOCFXAP-UHFFFAOYSA-N pictrelisib Chemical compound C1CN(S(=O)(=O)C)CCN1CC1=CC2=NC(C=3C=4C=NNC=4C=CC=3)=NC(N3CCOCC3)=C2S1 LHNIIDJUOCFXAP-UHFFFAOYSA-N 0.000 claims description 2
- 125000004194 piperazin-1-yl group Chemical group [H]N1C([H])([H])C([H])([H])N(*)C([H])([H])C1([H])[H] 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 229960002778 pyrvinium Drugs 0.000 claims description 2
- 229960001285 quercetin Drugs 0.000 claims description 2
- 235000005875 quercetin Nutrition 0.000 claims description 2
- XELRMPRLCPFTBH-UHFFFAOYSA-N quinazoline-2,4-diamine Chemical compound C1=CC=CC2=NC(N)=NC(N)=C21 XELRMPRLCPFTBH-UHFFFAOYSA-N 0.000 claims description 2
- MLKXDPUZXIRXEP-MFOYZWKCSA-N sulindac Chemical compound CC1=C(CC(O)=O)C2=CC(F)=CC=C2\C1=C/C1=CC=C(S(C)=O)C=C1 MLKXDPUZXIRXEP-MFOYZWKCSA-N 0.000 claims description 2
- 229960000894 sulindac Drugs 0.000 claims description 2
- 102100037293 Atrial natriuretic peptide-converting enzyme Human genes 0.000 claims 1
- 101000952934 Homo sapiens Atrial natriuretic peptide-converting enzyme Proteins 0.000 claims 1
- 102000007354 PAX6 Transcription Factor Human genes 0.000 claims 1
- 102000004495 STAT3 Transcription Factor Human genes 0.000 claims 1
- 101150092978 Slc25a4 gene Proteins 0.000 claims 1
- 108091008695 photoreceptors Proteins 0.000 claims 1
- QMHSXPLYMTVAMK-UHFFFAOYSA-N pyrvinium Chemical compound C1=CC2=CC(N(C)C)=CC=C2[N+](C)=C1\C=C\C(=C1C)C=C(C)N1C1=CC=CC=C1 QMHSXPLYMTVAMK-UHFFFAOYSA-N 0.000 claims 1
- 239000011159 matrix material Substances 0.000 description 11
- 208000002780 macular degeneration Diseases 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 4
- 206010064930 age-related macular degeneration Diseases 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000002356 single layer Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 238000004264 monolayer culture Methods 0.000 description 3
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- 208000010412 Glaucoma Diseases 0.000 description 2
- 208000035719 Maculopathy Diseases 0.000 description 2
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 description 2
- 208000014139 Retinal vascular disease Diseases 0.000 description 2
- 208000007014 Retinitis pigmentosa Diseases 0.000 description 2
- 102100024040 Signal transducer and activator of transcription 3 Human genes 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 108010015920 Type I Activin Receptors Proteins 0.000 description 2
- 102000002138 Type I Activin Receptors Human genes 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 229920000249 biocompatible polymer Polymers 0.000 description 2
- 208000030533 eye disease Diseases 0.000 description 2
- 239000000017 hydrogel Substances 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- LNAZSHAWQACDHT-XIYTZBAFSA-N (2r,3r,4s,5r,6s)-4,5-dimethoxy-2-(methoxymethyl)-3-[(2s,3r,4s,5r,6r)-3,4,5-trimethoxy-6-(methoxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6r)-4,5,6-trimethoxy-2-(methoxymethyl)oxan-3-yl]oxyoxane Chemical compound CO[C@@H]1[C@@H](OC)[C@H](OC)[C@@H](COC)O[C@H]1O[C@H]1[C@H](OC)[C@@H](OC)[C@H](O[C@H]2[C@@H]([C@@H](OC)[C@H](OC)O[C@@H]2COC)OC)O[C@@H]1COC LNAZSHAWQACDHT-XIYTZBAFSA-N 0.000 description 1
- NCYCYZXNIZJOKI-IOUUIBBYSA-N 11-cis-retinal Chemical compound O=C/C=C(\C)/C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-IOUUIBBYSA-N 0.000 description 1
- XSLHNXBPPDZDAU-UHFFFAOYSA-M 2-[(e)-2-(2,5-dimethyl-1-phenylpyrrol-3-yl)ethenyl]-n,n,1-trimethylquinolin-1-ium-6-amine;hydroxide Chemical compound [OH-].C1=CC2=CC(N(C)C)=CC=C2[N+](C)=C1\C=C\C(=C1C)C=C(C)N1C1=CC=CC=C1 XSLHNXBPPDZDAU-UHFFFAOYSA-M 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102100024504 Bone morphogenetic protein 3 Human genes 0.000 description 1
- 102100024505 Bone morphogenetic protein 4 Human genes 0.000 description 1
- 102100022525 Bone morphogenetic protein 6 Human genes 0.000 description 1
- 102100027052 Bone morphogenetic protein receptor type-1B Human genes 0.000 description 1
- ZEOWTGPWHLSLOG-UHFFFAOYSA-N Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F Chemical compound Cc1ccc(cc1-c1ccc2c(n[nH]c2c1)-c1cnn(c1)C1CC1)C(=O)Nc1cccc(c1)C(F)(F)F ZEOWTGPWHLSLOG-UHFFFAOYSA-N 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 102100028043 Fibroblast growth factor 3 Human genes 0.000 description 1
- 102100023593 Fibroblast growth factor receptor 1 Human genes 0.000 description 1
- 101710182386 Fibroblast growth factor receptor 1 Proteins 0.000 description 1
- 102100021262 Frizzled-3 Human genes 0.000 description 1
- 102000004437 G-Protein-Coupled Receptor Kinase 1 Human genes 0.000 description 1
- 108091004242 G-Protein-Coupled Receptor Kinase 1 Proteins 0.000 description 1
- 101100272733 Gallus gallus BRN3 gene Proteins 0.000 description 1
- 101100180045 Gallus gallus ISL1 gene Proteins 0.000 description 1
- 101000762375 Homo sapiens Bone morphogenetic protein 3 Proteins 0.000 description 1
- 101000762379 Homo sapiens Bone morphogenetic protein 4 Proteins 0.000 description 1
- 101000899390 Homo sapiens Bone morphogenetic protein 6 Proteins 0.000 description 1
- 101000899361 Homo sapiens Bone morphogenetic protein 7 Proteins 0.000 description 1
- 101000984546 Homo sapiens Bone morphogenetic protein receptor type-1B Proteins 0.000 description 1
- 101001060280 Homo sapiens Fibroblast growth factor 3 Proteins 0.000 description 1
- 101000819458 Homo sapiens Frizzled-3 Proteins 0.000 description 1
- 101001061405 Homo sapiens Frizzled-9 Proteins 0.000 description 1
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 description 1
- 101001094741 Homo sapiens POU domain, class 4, transcription factor 1 Proteins 0.000 description 1
- 101001094740 Homo sapiens POU domain, class 4, transcription factor 2 Proteins 0.000 description 1
- 101001094700 Homo sapiens POU domain, class 5, transcription factor 1 Proteins 0.000 description 1
- 101000729271 Homo sapiens Retinoid isomerohydrolase Proteins 0.000 description 1
- 101000666775 Homo sapiens T-box transcription factor TBX3 Proteins 0.000 description 1
- 101000701154 Homo sapiens Transcription factor ATOH7 Proteins 0.000 description 1
- 102100024392 Insulin gene enhancer protein ISL-1 Human genes 0.000 description 1
- 102100020677 Krueppel-like factor 4 Human genes 0.000 description 1
- 108010047294 Lamins Proteins 0.000 description 1
- 102100025744 Mothers against decapentaplegic homolog 1 Human genes 0.000 description 1
- 102100025751 Mothers against decapentaplegic homolog 2 Human genes 0.000 description 1
- 101710143123 Mothers against decapentaplegic homolog 2 Proteins 0.000 description 1
- 102000008730 Nestin Human genes 0.000 description 1
- 108010088225 Nestin Proteins 0.000 description 1
- 101100366602 Oryza sativa subsp. japonica SRFP1 gene Proteins 0.000 description 1
- 102100035395 POU domain, class 4, transcription factor 1 Human genes 0.000 description 1
- 102100035394 POU domain, class 4, transcription factor 2 Human genes 0.000 description 1
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 description 1
- 101150008375 Pou4f1 gene Proteins 0.000 description 1
- 102100026531 Prelamin-A/C Human genes 0.000 description 1
- 102100031176 Retinoid isomerohydrolase Human genes 0.000 description 1
- 102100040756 Rhodopsin Human genes 0.000 description 1
- 108090000820 Rhodopsin Proteins 0.000 description 1
- 108090000799 Rhodopsin kinases Proteins 0.000 description 1
- 101700032040 SMAD1 Proteins 0.000 description 1
- 102100033456 TGF-beta receptor type-1 Human genes 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 102100029372 Transcription factor ATOH7 Human genes 0.000 description 1
- 108010011702 Transforming Growth Factor-beta Type I Receptor Proteins 0.000 description 1
- 230000003592 biomimetic effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 210000005053 lamin Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 230000000921 morphogenic effect Effects 0.000 description 1
- 230000011234 negative regulation of signal transduction Effects 0.000 description 1
- 210000005055 nestin Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361888846P | 2013-10-09 | 2013-10-09 | |
| US61/888,846 | 2013-10-09 | ||
| PCT/US2014/059951 WO2015054526A2 (en) | 2013-10-09 | 2014-10-09 | Methods of mammalian retinal stem cell production and applications |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016537967A JP2016537967A (ja) | 2016-12-08 |
| JP2016537967A5 true JP2016537967A5 (https=) | 2017-11-16 |
| JP6651440B2 JP6651440B2 (ja) | 2020-02-19 |
Family
ID=52813744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016521740A Active JP6651440B2 (ja) | 2013-10-09 | 2014-10-09 | 哺乳動物の網膜幹細胞の生産方法及び適用 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US10220117B2 (https=) |
| EP (1) | EP3047017B1 (https=) |
| JP (1) | JP6651440B2 (https=) |
| CN (1) | CN105814192A (https=) |
| WO (1) | WO2015054526A2 (https=) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2147094B2 (en) * | 2007-04-18 | 2018-02-21 | Hadasit Medical Research Services & Development Limited | Stem cell-derived retinal pigment epithelial cells |
| WO2010096496A2 (en) | 2009-02-17 | 2010-08-26 | Memorial Sloan-Kettering Cancer Center | Methods of neural conversion of human embryonic stem cells |
| EP3693455A1 (en) | 2011-11-04 | 2020-08-12 | Memorial Sloan-Kettering Cancer Center | Midbrain dopamine (da) neurons for engraftment |
| EP3498824A1 (en) | 2013-04-26 | 2019-06-19 | Memorial Sloan-Kettering Cancer Center | Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells |
| TWI810142B (zh) | 2014-10-24 | 2023-08-01 | 日商住友製藥股份有限公司 | 神經組織的製造方法 |
| EP3211071B1 (en) | 2014-10-24 | 2021-05-05 | Sumitomo Dainippon Pharma Co., Ltd. | Production method for retinal tissue |
| US10626368B2 (en) * | 2015-04-14 | 2020-04-21 | Kyoto University | Method for inducing cerebral cortex neurons |
| WO2017015415A1 (en) * | 2015-07-22 | 2017-01-26 | The Johns Hopkins University | Three-dimensional vascular network assembly from induced pluripotent stem cells |
| AU2016318774B2 (en) | 2015-09-08 | 2022-08-18 | FUJIFILM Cellular Dynamics, Inc. | MACS-based purification of stem cell-derived retinal pigment epithelium |
| DK3347456T3 (da) * | 2015-09-08 | 2024-02-19 | Us Health | Fremgangsmåde til reproducerbar differentiering af retinale pigmentepitelceller af klinisk kvalitet |
| SG11201801770VA (en) * | 2015-09-08 | 2018-04-27 | Sumitomo Dainippon Pharma Co Ltd | Method for producing retinal pigment epithelial cells |
| US10947502B2 (en) | 2015-10-20 | 2021-03-16 | FUJIFILM Cellular Dynamics, Inc. | Methods for directed differentiation of pluripotent stem cells to immune cells |
| CN106609255B (zh) * | 2015-10-22 | 2019-11-05 | 同济大学 | 含pj34和视网膜色素上皮细胞的细胞悬液及其应用 |
| CN105274049B (zh) * | 2015-11-03 | 2018-01-09 | 中山大学中山眼科中心 | 一种诱导人iPSc源性的3D视网膜定向分化为视网膜神经节细胞的方法 |
| US20180362925A1 (en) * | 2015-12-04 | 2018-12-20 | Bond University Ltd | Methods for differentiating cells |
| SG11201809201UA (en) * | 2016-04-22 | 2018-11-29 | Sumitomo Dainippon Pharma Co Ltd | Method for producing retinal tissue |
| WO2017190136A1 (en) * | 2016-04-29 | 2017-11-02 | The Regents Of The University Of California | Induction of corneal endothelial cells |
| MX2019005476A (es) * | 2016-11-16 | 2019-07-04 | Cynata Therapeutics Ltd | Ensayo de celulas madre pluripotentes. |
| CN106754718A (zh) * | 2016-12-19 | 2017-05-31 | 胡其锐 | 一种人胚胎干细胞快速定向分化至视网膜色素上皮细胞的方法 |
| CN110520139A (zh) * | 2017-02-08 | 2019-11-29 | 哈达西特医疗研究服务和开发有限公司 | 用于治疗视网膜疾病的感光细胞 |
| WO2018164240A1 (ja) | 2017-03-08 | 2018-09-13 | 大日本住友製薬株式会社 | 網膜色素上皮細胞の製造方法 |
| SG11201909331UA (en) | 2017-04-18 | 2019-11-28 | Fujifilm Cellular Dynamics Inc | Antigen-specific immune effector cells |
| CN107326009B (zh) * | 2017-05-02 | 2019-03-08 | 深圳百年干细胞技术研究院有限公司 | 使血液单个核细胞逆向分化产生人血源性自体视网膜干细胞的方法、试剂盒及用途 |
| EP3638773A1 (en) * | 2017-06-16 | 2020-04-22 | IMBA-Institut für Molekulare Biotechnologie GmbH | Blood vessel organoid, methods of producing and using said organoids |
| WO2019069327A1 (en) | 2017-10-05 | 2019-04-11 | Eyestem Research Private Limited | UNIFIED CELL DIFFERENTIATION PROTOCOL |
| US12497591B2 (en) | 2018-03-07 | 2025-12-16 | Sorbonne Universite | Compositions and methods for efficient amplification of retinal progenitors cells |
| JP2021521792A (ja) * | 2018-04-20 | 2021-08-30 | フジフィルム セルラー ダイナミクス，インコーポレイテッド | 眼球細胞の分化方法及びその使用 |
| PT4234024T (pt) * | 2018-06-20 | 2026-01-21 | Biolamina Ab | Métodos e composições para produzir células do epitélio do pigmento da retina |
| TWI675678B (zh) * | 2018-08-23 | 2019-11-01 | 國為生醫科技股份有限公司 | 亞丁基苯酞於多巴胺神經前驅細胞移植治療的應用 |
| EP3847244B1 (en) * | 2018-09-07 | 2025-07-30 | Hebecell Corporation | Methods and compositions for retinal neuron generation in carrier-free 3d sphere suspension culture |
| CA3111848A1 (en) * | 2018-09-09 | 2020-03-12 | Qanatpharma Ag | Use of casein kinase 1 inhibitors for treating vascular diseases |
| US12427147B2 (en) | 2018-10-31 | 2025-09-30 | Senju Pharmaceutical Co., Ltd. | Retinal ganglion cell death inhibitor |
| GB201913196D0 (en) * | 2019-09-12 | 2019-10-30 | Univ Newcastle | Culture method |
| JPWO2022138803A1 (https=) * | 2020-12-24 | 2022-06-30 | ||
| CN113528441B (zh) * | 2021-08-05 | 2022-09-13 | 呈诺再生医学科技(珠海横琴新区)有限公司 | 快速高效的临床级色素上皮细胞诱导方法、试剂盒及应用 |
| US20250051724A1 (en) * | 2022-03-14 | 2025-02-13 | Space F Corporation | Medium composition for culturing porcine pluripotent stem cells |
| CN116445409B (zh) * | 2023-06-16 | 2023-09-22 | 中国人民解放军总医院第三医学中心 | 视杆感光前体细胞的制备方法及其应用 |
| CN116948970A (zh) * | 2023-08-21 | 2023-10-27 | 昆明理工大学 | 一种能够高效的获得足够数量的、高纯度人cr神经元的培养基、方法及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7632679B2 (en) | 2002-07-16 | 2009-12-15 | The Trustees Of Columbia University In The City Of New York | Systems and methods for screening for modulators of neural differentiation |
| US7541186B2 (en) | 2006-02-22 | 2009-06-02 | University Of Washington | Method of generating human retinal progenitors from embryonic stem cells |
| US20080089868A1 (en) | 2006-04-27 | 2008-04-17 | The Research Foundation Of State University Of New York | Retinal stem cell compositions and methods for preparing and using same |
| KR100846506B1 (ko) | 2006-12-19 | 2008-07-17 | 삼성전자주식회사 | Ｐｎ 다이오드를 포함하는 상변화 메모리 소자와 그 제조및 동작 방법 |
| EP2128244A4 (en) * | 2007-01-18 | 2012-05-09 | Riken | PROCESS FOR INDUCTION / DIFFERENTIATION TO A PHOTOREZEPTORZELLE |
| WO2010096496A2 (en) * | 2009-02-17 | 2010-08-26 | Memorial Sloan-Kettering Cancer Center | Methods of neural conversion of human embryonic stem cells |
| WO2011019092A1 (en) | 2009-08-12 | 2011-02-17 | Kyoto University | Method for inducing differentiation of pluripotent stem cells into neural precursor cells |
| CA2835215C (en) * | 2011-05-18 | 2023-10-10 | The Regents Of The University Of California | Non-immortal human retinal cell compositions, method for preparing them, and their use for treating retinal diseases |
| JP5985208B2 (ja) | 2011-11-25 | 2016-09-06 | 住友化学株式会社 | 網膜組織の製造方法 |
| ES2700980T3 (es) | 2012-02-17 | 2019-02-20 | Schepens Eye Res Inst | Perfil fenotípico de células progenitoras de retina humana |
-
2014
- 2014-10-09 EP EP14852550.4A patent/EP3047017B1/en active Active
- 2014-10-09 WO PCT/US2014/059951 patent/WO2015054526A2/en not_active Ceased
- 2014-10-09 CN CN201480066229.3A patent/CN105814192A/zh active Pending
- 2014-10-09 US US15/028,618 patent/US10220117B2/en active Active
- 2014-10-09 JP JP2016521740A patent/JP6651440B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2016537967A5 (https=) | ||
| JP6651440B2 (ja) | 哺乳動物の網膜幹細胞の生産方法及び適用 | |
| JP7023820B2 (ja) | 多分化能細胞および多能性細胞の分化を方向付けることによって発生させる皮質介在ニューロンおよびその他のニューロン細胞 | |
| JP2020178695A (ja) | 移植用中脳ドーパミン（ｄａ）ニューロン | |
| JP2018531011A (ja) | 中脳オルガノイドを作製するための手段及び方法 | |
| JP2018531011A6 (ja) | 中脳オルガノイドを作製するための手段及び方法 | |
| JP2023156413A (ja) | 背側化シグナル伝達物質又は腹側化シグナル伝達物質による錐体視細胞又は桿体視細胞の増加方法 | |
| CN105814194A (zh) | 产生视网膜色素上皮细胞的方法 | |
| EP3178925B1 (en) | Method for producing retinal ganglion cells | |
| WO2018062269A1 (ja) | 体細胞を製造する方法、体細胞、及び組成物 | |
| EP3384008A1 (en) | Methods of differentiating retinal cells | |
| KR20160033703A (ko) | 체세포로부터 신경 능선 세포로의 소분자 기반 전환 | |
| WO2017047797A1 (ja) | 膵芽細胞の製造方法 | |
| CN113272422A (zh) | 用于无载体3d球体悬浮培养中视网膜神经元生成的方法和组合物 | |
| US20250032551A1 (en) | Method for producing neural crest cells specialized for differentiation into mesenchymal lineage | |
| JP7414530B2 (ja) | 細胞凝集体、細胞凝集体の混合物及びそれらの製造方法 | |
| US20230392115A1 (en) | Methods for making and using differentiated neural cells | |
| WO2022239868A1 (ja) | 網膜組織の製造方法 | |
| US10174285B2 (en) | Methods of obtaining cell populations enriched with desired cells | |
| WO2015119575A2 (en) | Improved method, combination and/or composition for inducing cardiomyocyte or neuronal differentation | |
| CN121420058A (zh) | 增强神经祖细胞的神经元分化 | |
| US20220259558A1 (en) | Methods for obtaining eye field progenitor cells from human pluripotent stem cells | |
| CN121986159A (en) | Neural differentiation of pluripotent cells | |
| Corti | Uncoupling Tumorigenicity from Dopaminergic Differentiation Potential of HiPSCs by Acting on Glypican4: Combining in Vitro Differentiation Studies with Preclinical Studies for Parkinson's Disease Therapy | |
| WO2024157929A1 (ja) | 網膜組織の製造方法 |