JP2016521250A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2016521250A5 JP2016521250A5 JP2016501834A JP2016501834A JP2016521250A5 JP 2016521250 A5 JP2016521250 A5 JP 2016521250A5 JP 2016501834 A JP2016501834 A JP 2016501834A JP 2016501834 A JP2016501834 A JP 2016501834A JP 2016521250 A5 JP2016521250 A5 JP 2016521250A5
- Authority
- JP
- Japan
- Prior art keywords
- compound
- substrate
- dehalogenase
- mutant
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000001875 compounds Chemical class 0.000 claims description 61
- 239000000758 substrate Substances 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 23
- 108090000623 proteins and genes Proteins 0.000 claims description 17
- 102000004169 proteins and genes Human genes 0.000 claims description 17
- 125000000524 functional group Chemical group 0.000 claims description 16
- 238000006467 substitution reaction Methods 0.000 claims description 15
- 125000004429 atom Chemical group 0.000 claims description 14
- 239000003814 drug Substances 0.000 claims description 14
- 229940079593 drug Drugs 0.000 claims description 14
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 11
- 125000005647 linker group Chemical group 0.000 claims description 10
- 108020001507 fusion proteins Proteins 0.000 claims description 8
- 102000037865 fusion proteins Human genes 0.000 claims description 8
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical group NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 claims description 7
- 150000003384 small molecules Chemical class 0.000 claims description 7
- 150000002148 esters Chemical class 0.000 claims description 6
- 125000000217 alkyl group Chemical group 0.000 claims description 4
- 125000000539 amino acid group Chemical group 0.000 claims description 4
- 230000003100 immobilizing effect Effects 0.000 claims description 4
- 238000002372 labelling Methods 0.000 claims description 4
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 claims description 3
- 230000003993 interaction Effects 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims description 2
- 239000013592 cell lysate Substances 0.000 claims description 2
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 230000004927 fusion Effects 0.000 claims description 2
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 125000005843 halogen group Chemical group 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- -1 biomolecule Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361788257P | 2013-03-15 | 2013-03-15 | |
| US61/788,257 | 2013-03-15 | ||
| PCT/US2014/025359 WO2014151282A1 (en) | 2013-03-15 | 2014-03-13 | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016521250A JP2016521250A (ja) | 2016-07-21 |
| JP2016521250A5 true JP2016521250A5 (enExample) | 2017-04-20 |
| JP6604938B2 JP6604938B2 (ja) | 2019-11-13 |
Family
ID=51580924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016501834A Active JP6604938B2 (ja) | 2013-03-15 | 2014-03-13 | タンパク質を官能基または固体表面に共有結合的に係留するための基質 |
Country Status (4)
| Country | Link |
|---|---|
| US (3) | US11072812B2 (enExample) |
| EP (1) | EP2969435B1 (enExample) |
| JP (1) | JP6604938B2 (enExample) |
| WO (1) | WO2014151282A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104853767B (zh) | 2012-12-12 | 2017-04-05 | 普洛麦格公司 | 用于捕获生物活性剂的细胞标靶的组合物和方法 |
| US11072812B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| US10168323B2 (en) * | 2013-03-15 | 2019-01-01 | Promega Corporation | Compositions and methods for capture of cellular targets of bioactive agents |
| JP6876002B2 (ja) | 2015-06-05 | 2021-05-26 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー |
| JP2019512546A (ja) * | 2016-03-16 | 2019-05-16 | オリゾン・ゲノミクス・ソシエダッド・アノニマ | Ｋｄｍ１ａ標的会合を決定するための方法、およびそれに有用な化学プローブ |
| JP7496368B2 (ja) * | 2019-03-12 | 2024-06-06 | ノヴィラクス エルエルシー | 診療地点濃度分析器 |
| US11913944B2 (en) | 2019-03-20 | 2024-02-27 | Promega Corporation | Photoaffinity probes |
| US12371676B2 (en) | 2019-04-16 | 2025-07-29 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Circularly permutated haloalkane transferase fusion molecules |
| EP3816180A1 (en) | 2019-10-31 | 2021-05-05 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Circularly permutated haloalkane transferase fusion molecules |
| WO2021119149A1 (en) * | 2019-12-10 | 2021-06-17 | Promega Corporation | Compositions and methods for bioluminescent detection using multifunctional probes |
| JP2025516354A (ja) | 2022-05-04 | 2025-05-27 | プロメガ コーポレイション | スプリット修飾デハロゲナーゼバリアント |
| WO2023215514A2 (en) | 2022-05-04 | 2023-11-09 | Promega Corporation | Bioluminescence-triggered photocatalytic labeling |
| US20240132859A1 (en) | 2022-05-04 | 2024-04-25 | Promega Corporation | Modified dehalogenase with extended surface loop regions |
| EP4519290A1 (en) | 2022-05-04 | 2025-03-12 | Promega Corporation | Circularly permuted dehalogenase variants |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4812409A (en) | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Hydrolyzable fluorescent substrates and analytical determinations using same |
| US4810636A (en) | 1986-12-09 | 1989-03-07 | Miles Inc. | Chromogenic acridinone enzyme substrates |
| US6162931A (en) | 1996-04-12 | 2000-12-19 | Molecular Probes, Inc. | Fluorinated xanthene derivatives |
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| ES2369542T3 (es) | 2002-07-31 | 2011-12-01 | Seattle Genetics, Inc. | Conjugados de auristatina y su uso para tratar cáncer, una enfermedad autoinmune o una enfermedad infecciosa. |
| CA2499221A1 (en) | 2002-09-16 | 2004-03-25 | Promega Corporation | Rapidly degraded reporter fusion proteins |
| US7429472B2 (en) | 2003-01-31 | 2008-09-30 | Promega Corporation | Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule |
| KR20050109934A (ko) | 2003-01-31 | 2005-11-22 | 프로메가 코포레이션 | 관능기의 단백질로의 공유 테더링 |
| US20050187147A1 (en) | 2003-09-22 | 2005-08-25 | Newman Michael J. | Compositions and methods for increasing drug efficiency |
| US8293503B2 (en) | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| JP5089984B2 (ja) | 2003-10-03 | 2012-12-05 | プロメガ コーポレイション | 方向性クローニング用ベクター |
| WO2005038029A2 (en) | 2003-10-10 | 2005-04-28 | Promega Corporation | Luciferase biosensor |
| US7553632B2 (en) | 2004-01-22 | 2009-06-30 | Promega Corporation | Luminogenic and nonluminogenic multiplex assay |
| US7416854B2 (en) | 2004-01-22 | 2008-08-26 | Promega Corporation | Luminogenic and nonluminogenic multiplex assay |
| US7425436B2 (en) | 2004-07-30 | 2008-09-16 | Promega Corporation | Covalent tethering of functional groups to proteins and substrates therefor |
| US7728118B2 (en) | 2004-09-17 | 2010-06-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| EP1989548A2 (en) | 2006-02-08 | 2008-11-12 | Promega Corporation | Compositions and methods for capturing and analyzing cross-linked biomolecules |
| US9359635B2 (en) | 2006-04-03 | 2016-06-07 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| EP2374875B1 (en) | 2006-10-30 | 2015-09-02 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and funcitional expression |
| WO2008086035A2 (en) | 2007-01-10 | 2008-07-17 | Promega Corporation | Split mutant hydrolase fusion reporter and uses thereof |
| CN101679555B (zh) | 2007-05-23 | 2012-05-23 | 昭和电工株式会社 | 具有醚键的反应性氨基甲酸酯化合物、固化性组合物及固化物 |
| EP2626703B1 (en) | 2008-03-27 | 2017-05-03 | Promega Corporation | Protein labeling with cyanobenzothiazole conjugates |
| WO2010021686A1 (en) * | 2008-08-18 | 2010-02-25 | Promega Corporation | Luminogenic compounds and methods to detect cytochrome p450 3a enzymes |
| ES2795287T3 (es) | 2009-05-01 | 2020-11-23 | Promega Corp | Luciferasas de Oplophorus sintéticas con mayor emisión de luz |
| JP5730311B2 (ja) | 2009-08-31 | 2015-06-10 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 反応性シアニン化合物 |
| CN103443121B (zh) | 2010-11-02 | 2016-04-20 | 普罗梅加公司 | 新型腔肠素底物及其使用方法 |
| EP2649099A4 (en) * | 2010-12-07 | 2016-10-19 | Univ Yale | SMALL MOLECULAR HYDROPHOBIC LABELING OF FUSION PROTEINS AND INDUCED REMOVAL FROM THIS |
| WO2013078244A1 (en) | 2011-11-21 | 2013-05-30 | Promega Corporation | Carboxy x rhodamine analogs |
| CN104853767B (zh) | 2012-12-12 | 2017-04-05 | 普洛麦格公司 | 用于捕获生物活性剂的细胞标靶的组合物和方法 |
| US11072812B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
-
2014
- 2014-03-13 US US14/207,993 patent/US11072812B2/en active Active
- 2014-03-13 JP JP2016501834A patent/JP6604938B2/ja active Active
- 2014-03-13 WO PCT/US2014/025359 patent/WO2014151282A1/en not_active Ceased
- 2014-03-13 EP EP14769196.8A patent/EP2969435B1/en active Active
- 2014-03-13 US US14/207,959 patent/US11072811B2/en active Active
-
2021
- 2021-07-13 US US17/374,460 patent/US12221648B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12111320B2 (en) | 1,1′-[(substituted alkyl)imino]bis(alkylene)]bis-ferrocenes and their use in electrochemical assays by labelling substrates of interest | |
| Staub et al. | β-Lactams as selective chemical probes for the in vivo labeling of bacterial enzymes involved in cell wall biosynthesis, antibiotic resistance, and virulence | |
| JP2013078329A5 (enExample) | ||
| KR101319202B1 (ko) | ｐＬＤＨ에 특이적으로 결합하는 ＤＮＡ 압타머 | |
| JP2012523569A5 (enExample) | ||
| JP2009544660A5 (enExample) | ||
| JP2013539015A5 (enExample) | ||
| JP2016168062A5 (enExample) | ||
| JP2009536745A5 (enExample) | ||
| BR112013016240B1 (pt) | Novos marcadores de ferroceno para ensaio eletroquímico e seu uso em métodos analíticos | |
| JP2010141309A5 (ja) | 発光素子 | |
| GT200600306A (es) | Inhibidores de glutamato agrecanasa | |
| EA201490709A1 (ru) | Адъювантное соединение | |
| NZ595303A (en) | Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides | |
| JP2014511846A5 (enExample) | ||
| DK1187933T3 (da) | Betalactam-indeholdende forbindelser | |
| RU2020129667A (ru) | Способ детектирования молекулы-мишени и набор для использования в этом способе | |
| WO2016196956A1 (en) | Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements | |
| CN103313724A (zh) | 化合物和方法 | |
| ATE540051T1 (de) | Age-inhibitoren | |
| JP2017006122A5 (enExample) | ||
| KR20200025249A (ko) | Dehp 검출을 위한 앱타머 및 이를 이용한 dehp 검출장치 | |
| JP2007045816A5 (ja) | カルバゾール誘導体、発光素子用材料として用いられるアントラセン誘導体の作製方法 | |
| JP2014508281A (ja) | グルコースセンサ分子 | |
| CN104262250A (zh) | 三碘代甲状腺素半抗原发光标记物及合成方法 |