JP6604938B2 - タンパク質を官能基または固体表面に共有結合的に係留するための基質 - Google Patents

タンパク質を官能基または固体表面に共有結合的に係留するための基質 Download PDF

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JP6604938B2
JP6604938B2 JP2016501834A JP2016501834A JP6604938B2 JP 6604938 B2 JP6604938 B2 JP 6604938B2 JP 2016501834 A JP2016501834 A JP 2016501834A JP 2016501834 A JP2016501834 A JP 2016501834A JP 6604938 B2 JP6604938 B2 JP 6604938B2
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substrate
protein
dehalogenase
mutant
cell
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JP2016521250A (ja
JP2016521250A5 (enExample
Inventor
カロリン ダブリュ ヒトコ
トーマス カークランド
セルジー レヴィン
ポンチョ マイゼンハイマー
レイチェル フリードマン オハナ
ハリー テツオ ウエダ
ジ ジュー
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Promega Corp
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Promega Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Steroid Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
JP2016501834A 2013-03-15 2014-03-13 タンパク質を官能基または固体表面に共有結合的に係留するための基質 Active JP6604938B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361788257P 2013-03-15 2013-03-15
US61/788,257 2013-03-15
PCT/US2014/025359 WO2014151282A1 (en) 2013-03-15 2014-03-13 Substrates for covalent tethering of proteins to functional groups or solid surfaces

Publications (3)

Publication Number Publication Date
JP2016521250A JP2016521250A (ja) 2016-07-21
JP2016521250A5 JP2016521250A5 (enExample) 2017-04-20
JP6604938B2 true JP6604938B2 (ja) 2019-11-13

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JP2016501834A Active JP6604938B2 (ja) 2013-03-15 2014-03-13 タンパク質を官能基または固体表面に共有結合的に係留するための基質

Country Status (4)

Country Link
US (3) US11072811B2 (enExample)
EP (1) EP2969435B1 (enExample)
JP (1) JP6604938B2 (enExample)
WO (1) WO2014151282A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102272233B1 (ko) * 2012-12-12 2021-07-01 프로메가 코포레이션 생체활성 제제의 세포 표적 포획을 위한 조성물 및 방법
US10168323B2 (en) * 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
JP6876002B2 (ja) 2015-06-05 2021-05-26 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー
ES3042059T3 (en) 2016-03-16 2025-11-18 Oryzon Genomics Sa Methods to determine kdm1a target engagement and chemoprobes useful therefor
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US12371676B2 (en) 2019-04-16 2025-07-29 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Circularly permutated haloalkane transferase fusion molecules
EP3816180A1 (en) 2019-10-31 2021-05-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Circularly permutated haloalkane transferase fusion molecules
EP4073512B1 (en) * 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515179A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 円順列置換デハロゲナーゼバリアント
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
JP2025515181A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
CA2802205C (en) 2002-07-31 2016-01-19 Seattle Genetics, Inc. Drug conjugates and their use for treating cancer, an autoimmune disease or an infectious disease
WO2004025264A2 (en) 2002-09-16 2004-03-25 Promega Corporation Rapidly degraded reporter fusion proteins
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
US20050187147A1 (en) * 2003-09-22 2005-08-25 Newman Michael J. Compositions and methods for increasing drug efficiency
WO2005087932A2 (en) 2003-10-03 2005-09-22 Promega Corporation Vectors for directional cloning
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP2298902B1 (en) 2003-10-10 2015-04-22 Promega Corporation Luciferase biosensor
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
EP1989548A2 (en) 2006-02-08 2008-11-12 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
JP5684984B2 (ja) 2006-04-03 2015-03-18 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 順列置換及び非順列置換ルシフェラーゼバイオセンサー
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
JPWO2008146685A1 (ja) 2007-05-23 2010-08-19 昭和電工株式会社 エーテル結合を有する反応性ウレタン化合物、硬化性組成物および硬化物
EP3141905B1 (en) 2008-03-27 2019-03-06 Promega Corporation Protein labeling with cyanobenzothiazole conjugates
JP5597200B2 (ja) 2008-08-18 2014-10-01 プロメガ コーポレイション ルミネッセンスを生じる化合物およびチトクロムｐ４５０３ａ酵素を検出するための方法
KR101665352B1 (ko) 2009-05-01 2016-10-12 프로메가 코포레이션 광 출력이 향상된 합성 오플로포러스 루시퍼라제
EP2473563B1 (en) 2009-08-31 2019-06-19 Promega Corporation Reactive cyanine compounds
AU2011323418B2 (en) 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
EP2649099A4 (en) * 2010-12-07 2016-10-19 Univ Yale SMALL MOLECULAR HYDROPHOBIC LABELING OF FUSION PROTEINS AND INDUCED REMOVAL FROM THIS
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
KR102272233B1 (ko) 2012-12-12 2021-07-01 프로메가 코포레이션 생체활성 제제의 세포 표적 포획을 위한 조성물 및 방법
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces

Also Published As

Publication number Publication date
US11072812B2 (en) 2021-07-27
US20140322738A1 (en) 2014-10-30
US12221648B2 (en) 2025-02-11
JP2016521250A (ja) 2016-07-21
US20210340593A1 (en) 2021-11-04
US20140322794A1 (en) 2014-10-30
EP2969435A4 (en) 2016-10-05
EP2969435B1 (en) 2021-11-03
EP2969435A1 (en) 2016-01-20
US11072811B2 (en) 2021-07-27
WO2014151282A1 (en) 2014-09-25

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