JP6604938B2 - タンパク質を官能基または固体表面に共有結合的に係留するための基質 - Google Patents

タンパク質を官能基または固体表面に共有結合的に係留するための基質 Download PDF

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JP6604938B2
JP6604938B2 JP2016501834A JP2016501834A JP6604938B2 JP 6604938 B2 JP6604938 B2 JP 6604938B2 JP 2016501834 A JP2016501834 A JP 2016501834A JP 2016501834 A JP2016501834 A JP 2016501834A JP 6604938 B2 JP6604938 B2 JP 6604938B2
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substrate
protein
dehalogenase
mutant
cell
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JP2016521250A (ja
JP2016521250A5 (enExample
Inventor
カロリン ダブリュ ヒトコ
トーマス カークランド
セルジー レヴィン
ポンチョ マイゼンハイマー
レイチェル フリードマン オハナ
ハリー テツオ ウエダ
ジ ジュー
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Promega Corp
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Promega Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Steroid Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP2016501834A 2013-03-15 2014-03-13 タンパク質を官能基または固体表面に共有結合的に係留するための基質 Active JP6604938B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361788257P 2013-03-15 2013-03-15
US61/788,257 2013-03-15
PCT/US2014/025359 WO2014151282A1 (en) 2013-03-15 2014-03-13 Substrates for covalent tethering of proteins to functional groups or solid surfaces

Publications (3)

Publication Number Publication Date
JP2016521250A JP2016521250A (ja) 2016-07-21
JP2016521250A5 JP2016521250A5 (enExample) 2017-04-20
JP6604938B2 true JP6604938B2 (ja) 2019-11-13

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JP2016501834A Active JP6604938B2 (ja) 2013-03-15 2014-03-13 タンパク質を官能基または固体表面に共有結合的に係留するための基質

Country Status (4)

Country Link
US (3) US11072812B2 (enExample)
EP (1) EP2969435B1 (enExample)
JP (1) JP6604938B2 (enExample)
WO (1) WO2014151282A1 (enExample)

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JP6484178B2 (ja) 2012-12-12 2019-03-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生物活性剤の細胞標的の捕捉のための組成物および方法
US10168323B2 (en) * 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US11072812B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
EP3304078B1 (en) * 2015-06-05 2022-08-31 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
WO2017158136A1 (en) 2016-03-16 2017-09-21 Oryzon Genomics, S.A. Methods to determine kdm1a target engagement and chemoprobes useful therefor
CA3133265A1 (en) * 2019-03-12 2020-09-17 Novilux Llc Point of care concentration analyzer
WO2020191339A1 (en) 2019-03-20 2020-09-24 Promega Corporation Photoaffinity probes
JP7599074B2 (ja) 2019-04-16 2024-12-13 マックス－プランク－ゲゼルシャフト ツール フェルデルンク デル ヴィッセンシャフテン エー．ファウ． 円順列変異ハロアルカントランスフェラーゼ融合分子
EP3816180A1 (en) 2019-10-31 2021-05-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Circularly permutated haloalkane transferase fusion molecules
EP4073512B1 (en) * 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
JP2025516354A (ja) 2022-05-04 2025-05-27 プロメガ コーポレイション スプリット修飾デハロゲナーゼバリアント
EP4519290A1 (en) 2022-05-04 2025-03-12 Promega Corporation Circularly permuted dehalogenase variants
WO2023215505A1 (en) 2022-05-04 2023-11-09 Promega Corporation Modified dehalogenase with extended surface loop regions

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US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
ES2544527T3 (es) * 2002-07-31 2015-09-01 Seattle Genetics, Inc. Conjugados de fármacos y su uso para tratar el cáncer, una enfermedad autoinmune o una enfermedad infecciosa
JP4528623B2 (ja) 2002-09-16 2010-08-18 プロメガ コーポレイション 迅速分解性レポーター融合タンパク質
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
EP1594962B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
WO2005035003A2 (en) 2003-09-22 2005-04-21 Dihedron Corporation Compositions and methods for increasing drug efficiency
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
ATE442445T1 (de) 2003-10-03 2009-09-15 Promega Corp Rhamnose-induzierbares expressionssystem
WO2005038029A2 (en) 2003-10-10 2005-04-28 Promega Corporation Luciferase biosensor
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
WO2007092579A2 (en) 2006-02-08 2007-08-16 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
EP2004813B1 (en) 2006-04-03 2015-06-03 Promega Corporation Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides
CA2667697A1 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
JPWO2008146685A1 (ja) * 2007-05-23 2010-08-19 昭和電工株式会社 エーテル結合を有する反応性ウレタン化合物、硬化性組成物および硬化物
WO2009142678A1 (en) 2008-03-27 2009-11-26 Promega Corporatin Protein labeling with cyanobenzothiazole conjugates
US8288559B2 (en) * 2008-08-18 2012-10-16 Promega Corporation Luminogenic compounds and methods to detect cytochrome P450 3A enzymes
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JP5730311B2 (ja) 2009-08-31 2015-06-10 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 反応性シアニン化合物
US8669103B2 (en) 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
AU2011338615B2 (en) 2010-12-07 2017-07-27 Yale University Small-molecule hydrophobic tagging of fusion proteins and induced degradation of same
JP6522339B2 (ja) 2011-11-21 2019-05-29 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ カルボキシｘローダミン類似体
JP6484178B2 (ja) 2012-12-12 2019-03-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生物活性剤の細胞標的の捕捉のための組成物および方法
US11072812B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces

Also Published As

Publication number Publication date
JP2016521250A (ja) 2016-07-21
US20140322794A1 (en) 2014-10-30
US11072812B2 (en) 2021-07-27
US11072811B2 (en) 2021-07-27
WO2014151282A1 (en) 2014-09-25
EP2969435A1 (en) 2016-01-20
US20140322738A1 (en) 2014-10-30
EP2969435A4 (en) 2016-10-05
US12221648B2 (en) 2025-02-11
US20210340593A1 (en) 2021-11-04
EP2969435B1 (en) 2021-11-03

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