JP2016516412A5 - - Google Patents
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- JP2016516412A5 JP2016516412A5 JP2016503404A JP2016503404A JP2016516412A5 JP 2016516412 A5 JP2016516412 A5 JP 2016516412A5 JP 2016503404 A JP2016503404 A JP 2016503404A JP 2016503404 A JP2016503404 A JP 2016503404A JP 2016516412 A5 JP2016516412 A5 JP 2016516412A5
- Authority
- JP
- Japan
- Prior art keywords
- temperature
- promoter
- desired protein
- cell
- eukaryotic cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims 25
- 108090000623 proteins and genes Proteins 0.000 claims 18
- 235000018102 proteins Nutrition 0.000 claims 12
- 102000004169 proteins and genes Human genes 0.000 claims 12
- 210000003527 eukaryotic cell Anatomy 0.000 claims 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 6
- 230000001939 inductive effect Effects 0.000 claims 5
- 238000012258 culturing Methods 0.000 claims 4
- 102100036826 Aldehyde oxidase Human genes 0.000 claims 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 3
- 101000928314 Homo sapiens Aldehyde oxidase Proteins 0.000 claims 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 3
- 229910052799 carbon Inorganic materials 0.000 claims 3
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims 3
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 claims 2
- 101150069554 HIS4 gene Proteins 0.000 claims 2
- 101100502336 Komagataella pastoris FLD1 gene Proteins 0.000 claims 2
- 241000235648 Pichia Species 0.000 claims 2
- 101100421128 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SEI1 gene Proteins 0.000 claims 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims 2
- LXJXRIRHZLFYRP-UHFFFAOYSA-N glyceraldehyde 3-phosphate Chemical compound O=CC(O)COP(O)(O)=O LXJXRIRHZLFYRP-UHFFFAOYSA-N 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 235000019157 thiamine Nutrition 0.000 claims 2
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims 2
- 229960003495 thiamine Drugs 0.000 claims 2
- 239000011721 thiamine Substances 0.000 claims 2
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 claims 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims 1
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 claims 1
- 241000271566 Aves Species 0.000 claims 1
- 108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 claims 1
- 102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 claims 1
- 102100039556 Galectin-4 Human genes 0.000 claims 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 claims 1
- 102100021866 Hepatocyte growth factor Human genes 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 claims 1
- 101000608765 Homo sapiens Galectin-4 Proteins 0.000 claims 1
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims 1
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 claims 1
- 101001098868 Homo sapiens Proprotein convertase subtilisin/kexin type 9 Proteins 0.000 claims 1
- 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 claims 1
- 102000004889 Interleukin-6 Human genes 0.000 claims 1
- 108090001005 Interleukin-6 Proteins 0.000 claims 1
- 101710122479 Isocitrate lyase 1 Proteins 0.000 claims 1
- 241000235058 Komagataella pastoris Species 0.000 claims 1
- 241000270322 Lepidosauria Species 0.000 claims 1
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 1
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 1
- 101150012394 PHO5 gene Proteins 0.000 claims 1
- 208000020584 Polyploidy Diseases 0.000 claims 1
- 102100038955 Proprotein convertase subtilisin/kexin type 9 Human genes 0.000 claims 1
- 101100191147 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PHO3 gene Proteins 0.000 claims 1
- 239000004098 Tetracycline Substances 0.000 claims 1
- 208000035199 Tetraploidy Diseases 0.000 claims 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims 1
- 230000002159 abnormal effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 210000000805 cytoplasm Anatomy 0.000 claims 1
- 210000001840 diploid cell Anatomy 0.000 claims 1
- 238000001502 gel electrophoresis Methods 0.000 claims 1
- 230000013595 glycosylation Effects 0.000 claims 1
- 238000006206 glycosylation reaction Methods 0.000 claims 1
- 210000003783 haploid cell Anatomy 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 101150061302 och1 gene Proteins 0.000 claims 1
- 210000001850 polyploid cell Anatomy 0.000 claims 1
- 101150015622 pyk gene Proteins 0.000 claims 1
- 238000001542 size-exclusion chromatography Methods 0.000 claims 1
- 229960002180 tetracycline Drugs 0.000 claims 1
- 229930101283 tetracycline Natural products 0.000 claims 1
- 235000019364 tetracycline Nutrition 0.000 claims 1
- 150000003522 tetracyclines Chemical class 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361790613P | 2013-03-15 | 2013-03-15 | |
| US201361791471P | 2013-03-15 | 2013-03-15 | |
| US61/790,613 | 2013-03-15 | ||
| US61/791,471 | 2013-03-15 | ||
| PCT/US2014/030453 WO2014145650A1 (en) | 2013-03-15 | 2014-03-17 | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016516412A JP2016516412A (ja) | 2016-06-09 |
| JP2016516412A5 true JP2016516412A5 (https=) | 2017-03-16 |
| JP6412552B2 JP6412552B2 (ja) | 2018-10-24 |
Family
ID=51538058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016503404A Active JP6412552B2 (ja) | 2013-03-15 | 2014-03-17 | 酵母及び他の形質転換細胞中のポリペプチドの高収率発現のための温度シフト |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US10138294B2 (https=) |
| EP (2) | EP2971037B1 (https=) |
| JP (1) | JP6412552B2 (https=) |
| KR (1) | KR102202477B1 (https=) |
| CN (1) | CN105121652B (https=) |
| AU (1) | AU2014232813B2 (https=) |
| CA (1) | CA2905180C (https=) |
| DK (1) | DK2971037T3 (https=) |
| ES (2) | ES3057435T3 (https=) |
| IL (1) | IL241176B (https=) |
| MX (2) | MX366647B (https=) |
| SG (1) | SG11201506034TA (https=) |
| TW (1) | TWI636136B (https=) |
| WO (1) | WO2014145650A1 (https=) |
| ZA (1) | ZA201505483B (https=) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10202630B2 (en) * | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| PE20161439A1 (es) | 2014-03-21 | 2017-01-26 | Teva Pharmaceuticals Int Gmbh | Anticuerpos antagonistas dirigidos contra el peptido relacionado con el gen de calcitonina y metodos que usan los mismos |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| JP2018501814A (ja) | 2014-11-11 | 2018-01-25 | クララ フーズ カンパニー | 卵白タンパク質産生のための方法および組成物 |
| JP2017045865A (ja) | 2015-08-26 | 2017-03-02 | ルネサスエレクトロニクス株式会社 | 半導体装置およびその製造方法 |
| CN106191181B (zh) * | 2016-07-22 | 2019-12-13 | 江苏江山聚源生物技术有限公司 | 一种毕赤酵母表达重组蛋白的发酵工艺 |
| WO2018055574A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
| EP3592800A4 (en) | 2017-03-10 | 2021-01-06 | Bolt Threads, Inc. | COMPOSITIONS AND METHODS OF PRODUCING HIGHLY SECRETED YIELD OF RECOMBINANT PROTEIN |
| KR20250121449A (ko) | 2017-03-10 | 2025-08-12 | 볼트 쓰레즈, 인크. | 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 |
| IL277352B2 (en) * | 2018-03-16 | 2025-08-01 | Genzyme Corp | Methods for improving cell viability in a production bioreactor |
| US10221403B1 (en) * | 2018-04-25 | 2019-03-05 | Life Rainbow Biotech Co., Ltd. | Method of preparing zearalenone hydrolase |
| CN108546637A (zh) * | 2018-05-23 | 2018-09-18 | 华东理工大学 | 重组毕赤酵母表达葡萄糖氧化酶发酵装置以及发酵培养方法 |
| MX2021000053A (es) * | 2018-07-12 | 2021-03-25 | Amyris Inc | Metodos para controlar velocidades de alimentacion de la fermentacion. |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| BR112022000545A2 (pt) | 2019-07-11 | 2022-04-05 | Clara Foods Co | Composições de proteína e produtos de consumo destas |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| FI4055177T3 (fi) * | 2019-11-08 | 2024-10-31 | Danisco Us Inc | Parantuneen proteiinituottavuuden fenotyyppejä käsittäviä sienikantoja ja menetelmiä niitä varten |
| WO2021146233A1 (en) | 2020-01-14 | 2021-07-22 | Basf Se | Method for producing recombinant protein in yeast cells |
| US20250207158A1 (en) * | 2022-03-24 | 2025-06-26 | Versalis S.P.A. | Fermentation process for the production of lipids from oleaginous yeasts |
| EP4594342A2 (en) * | 2022-09-29 | 2025-08-06 | Antheia, Inc. | Methods of improving production of morphinan alkaloids and derivatives |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| ES2251723T3 (es) | 1994-08-12 | 2006-05-01 | Immunomedics, Inc. | Inmunoconjugados y anticuerpos humanizados especificos para linfoma de celulas b y celulas de leucemia. |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6221630B1 (en) | 1999-03-24 | 2001-04-24 | The Penn State Research Foundation | High copy number recombinant expression construct for regulated high-level production of polypeptides in yeast |
| PT1678314E (pt) | 2003-10-22 | 2012-11-27 | Keck Graduate Inst | Métodos de síntese de polipéptidos heteromultiméricos em levedura utilizando uma estratégia de conjugação haplóide |
| DK2021463T3 (en) | 2006-05-19 | 2017-01-16 | Alder Biopharmaceuticals Inc | Culture method to obtain a cloned population of antigen-specific B cells |
| HUE032563T2 (en) | 2007-05-21 | 2017-09-28 | Alderbio Holdings Llc | Antibodies to IL-6 and their use |
| KR20170036814A (ko) | 2007-05-21 | 2017-04-03 | 앨더바이오 홀딩스 엘엘씨 | 신규한 래빗 항체 인간화 방법 및 인간화된 래빗 항체 |
| US20090269832A1 (en) | 2007-11-05 | 2009-10-29 | Modular Genetics, Inc. | Growth of Microorganisms in Media Containing Crude Glycerol |
| US8501449B2 (en) | 2007-12-04 | 2013-08-06 | Proteon Therapeutics, Inc. | Recombinant elastase proteins and methods of manufacturing and use thereof |
| US8609416B2 (en) | 2009-12-18 | 2013-12-17 | Ventria Bioscience | Methods and compositions comprising heat shock proteins |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| US10150968B2 (en) | 2011-08-19 | 2018-12-11 | Alderbio Holdings Llc | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| EP2744903B1 (en) | 2011-08-19 | 2018-10-10 | AlderBio Holdings LLC | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
| US10202630B2 (en) * | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| US10189892B2 (en) * | 2013-03-15 | 2019-01-29 | Alderbio Holdings Llc | Fermentation process for antibody production |
-
2014
- 2014-03-17 CA CA2905180A patent/CA2905180C/en active Active
- 2014-03-17 TW TW103109931A patent/TWI636136B/zh active
- 2014-03-17 ES ES19177519T patent/ES3057435T3/es active Active
- 2014-03-17 EP EP14764393.6A patent/EP2971037B1/en active Active
- 2014-03-17 MX MX2015011968A patent/MX366647B/es active IP Right Grant
- 2014-03-17 MX MX2019008417A patent/MX384491B/es unknown
- 2014-03-17 WO PCT/US2014/030453 patent/WO2014145650A1/en not_active Ceased
- 2014-03-17 US US14/776,892 patent/US10138294B2/en active Active
- 2014-03-17 EP EP19177519.6A patent/EP3597664B1/en active Active
- 2014-03-17 ES ES14764393T patent/ES2749751T3/es active Active
- 2014-03-17 DK DK14764393.6T patent/DK2971037T3/da active
- 2014-03-17 AU AU2014232813A patent/AU2014232813B2/en active Active
- 2014-03-17 CN CN201480014647.8A patent/CN105121652B/zh active Active
- 2014-03-17 JP JP2016503404A patent/JP6412552B2/ja active Active
- 2014-03-17 KR KR1020157028401A patent/KR102202477B1/ko active Active
- 2014-03-17 SG SG11201506034TA patent/SG11201506034TA/en unknown
-
2015
- 2015-07-30 ZA ZA2015/05483A patent/ZA201505483B/en unknown
- 2015-09-03 IL IL241176A patent/IL241176B/en active IP Right Grant
-
2018
- 2018-10-24 US US16/169,130 patent/US11447540B2/en active Active
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