CN105121652B - 用于在酵母和其它转化细胞中高产量表达多肽的温度变动法 - Google Patents

用于在酵母和其它转化细胞中高产量表达多肽的温度变动法 Download PDF

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CN105121652B
CN105121652B CN201480014647.8A CN201480014647A CN105121652B CN 105121652 B CN105121652 B CN 105121652B CN 201480014647 A CN201480014647 A CN 201480014647A CN 105121652 B CN105121652 B CN 105121652B
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antibody
temperature
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yeast
cell
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CN105121652A (zh
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G·莱斯尼基
P·D·麦克内尔
F·哈特内尔
M·杨
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H Lundbeck AS
Lundbeck Seattle Biopharmaceuticals Inc
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Alder Biopharmaceuticals Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/248—IL-6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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CN201480014647.8A 2013-03-15 2014-03-17 用于在酵母和其它转化细胞中高产量表达多肽的温度变动法 Active CN105121652B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361790613P 2013-03-15 2013-03-15
US201361791471P 2013-03-15 2013-03-15
US61/790,613 2013-03-15
US61/791,471 2013-03-15
PCT/US2014/030453 WO2014145650A1 (en) 2013-03-15 2014-03-17 Temperature shift for high yield expression of polypeptides in yeast and other transformed cells

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CN105121652A CN105121652A (zh) 2015-12-02
CN105121652B true CN105121652B (zh) 2020-07-28

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US (2) US10138294B2 (https=)
EP (2) EP2971037B1 (https=)
JP (1) JP6412552B2 (https=)
KR (1) KR102202477B1 (https=)
CN (1) CN105121652B (https=)
AU (1) AU2014232813B2 (https=)
CA (1) CA2905180C (https=)
DK (1) DK2971037T3 (https=)
ES (2) ES3057435T3 (https=)
IL (1) IL241176B (https=)
MX (2) MX366647B (https=)
SG (1) SG11201506034TA (https=)
TW (1) TWI636136B (https=)
WO (1) WO2014145650A1 (https=)
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US10556945B2 (en) 2014-03-21 2020-02-11 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
JP2017045865A (ja) 2015-08-26 2017-03-02 ルネサスエレクトロニクス株式会社 半導体装置およびその製造方法
CN106191181B (zh) * 2016-07-22 2019-12-13 江苏江山聚源生物技术有限公司 一种毕赤酵母表达重组蛋白的发酵工艺
WO2018055574A1 (en) 2016-09-23 2018-03-29 Teva Pharmaceuticals International Gmbh Treating refractory migraine
EP3592800A4 (en) 2017-03-10 2021-01-06 Bolt Threads, Inc. COMPOSITIONS AND METHODS OF PRODUCING HIGHLY SECRETED YIELD OF RECOMBINANT PROTEIN
KR20250121449A (ko) 2017-03-10 2025-08-12 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
IL277352B2 (en) * 2018-03-16 2025-08-01 Genzyme Corp Methods for improving cell viability in a production bioreactor
US10221403B1 (en) * 2018-04-25 2019-03-05 Life Rainbow Biotech Co., Ltd. Method of preparing zearalenone hydrolase
CN108546637A (zh) * 2018-05-23 2018-09-18 华东理工大学 重组毕赤酵母表达葡萄糖氧化酶发酵装置以及发酵培养方法
MX2021000053A (es) * 2018-07-12 2021-03-25 Amyris Inc Metodos para controlar velocidades de alimentacion de la fermentacion.
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
FI4055177T3 (fi) * 2019-11-08 2024-10-31 Danisco Us Inc Parantuneen proteiinituottavuuden fenotyyppejä käsittäviä sienikantoja ja menetelmiä niitä varten
WO2021146233A1 (en) 2020-01-14 2021-07-22 Basf Se Method for producing recombinant protein in yeast cells
US20250207158A1 (en) * 2022-03-24 2025-06-26 Versalis S.P.A. Fermentation process for the production of lipids from oleaginous yeasts
EP4594342A2 (en) * 2022-09-29 2025-08-06 Antheia, Inc. Methods of improving production of morphinan alkaloids and derivatives

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TWI636136B (zh) 2018-09-21
TW201512401A (zh) 2015-04-01
WO2014145650A1 (en) 2014-09-18
EP3597664A2 (en) 2020-01-22
ES3057435T3 (en) 2026-03-02
EP2971037A1 (en) 2016-01-20
US10138294B2 (en) 2018-11-27
US11447540B2 (en) 2022-09-20
US20160039911A1 (en) 2016-02-11
MX366647B (es) 2019-07-17
EP3597664C0 (en) 2025-11-26
EP2971037B1 (en) 2019-07-10
JP2016516412A (ja) 2016-06-09
SG11201506034TA (en) 2015-09-29
EP3597664B1 (en) 2025-11-26
IL241176A0 (en) 2015-11-30
MX2015011968A (es) 2017-01-06
CA2905180A1 (en) 2014-09-18
MX384491B (es) 2025-03-14
US20190119360A1 (en) 2019-04-25
AU2014232813B2 (en) 2017-12-07
AU2014232813A1 (en) 2015-09-03
CN105121652A (zh) 2015-12-02
NZ711400A (en) 2021-05-28
CA2905180C (en) 2023-03-07
DK2971037T3 (da) 2019-10-14
ES2749751T3 (es) 2020-03-23
EP2971037A4 (en) 2016-07-20
EP3597664A3 (en) 2020-03-11
MX2019008417A (es) 2019-09-13
KR20150140678A (ko) 2015-12-16
KR102202477B1 (ko) 2021-01-13
ZA201505483B (en) 2022-08-31
IL241176B (en) 2020-05-31
JP6412552B2 (ja) 2018-10-24

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