KR102202477B1 - 효모 및 기타 형질전환 세포에서 폴리펩티드의 고수율 발현을 위한 온도 전환 - Google Patents

효모 및 기타 형질전환 세포에서 폴리펩티드의 고수율 발현을 위한 온도 전환 Download PDF

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KR102202477B1
KR102202477B1 KR1020157028401A KR20157028401A KR102202477B1 KR 102202477 B1 KR102202477 B1 KR 102202477B1 KR 1020157028401 A KR1020157028401 A KR 1020157028401A KR 20157028401 A KR20157028401 A KR 20157028401A KR 102202477 B1 KR102202477 B1 KR 102202477B1
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temperature
antibody
promoter
yeast
protein
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KR20150140678A (ko
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게리 레스니키
패트리샤 다이앤 맥닐
프란츠 하트너
마크 영
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룬드벡 시애틀 바이오파마슈티컬즈, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C07K16/248—IL-6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2523/00—Culture process characterised by temperature

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analytical Chemistry (AREA)
KR1020157028401A 2013-03-15 2014-03-17 효모 및 기타 형질전환 세포에서 폴리펩티드의 고수율 발현을 위한 온도 전환 Active KR102202477B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361790613P 2013-03-15 2013-03-15
US201361791471P 2013-03-15 2013-03-15
US61/790,613 2013-03-15
US61/791,471 2013-03-15
PCT/US2014/030453 WO2014145650A1 (en) 2013-03-15 2014-03-17 Temperature shift for high yield expression of polypeptides in yeast and other transformed cells

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KR20150140678A KR20150140678A (ko) 2015-12-16
KR102202477B1 true KR102202477B1 (ko) 2021-01-13

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US (2) US10138294B2 (https=)
EP (2) EP2971037B1 (https=)
JP (1) JP6412552B2 (https=)
KR (1) KR102202477B1 (https=)
CN (1) CN105121652B (https=)
AU (1) AU2014232813B2 (https=)
CA (1) CA2905180C (https=)
DK (1) DK2971037T3 (https=)
ES (2) ES3057435T3 (https=)
IL (1) IL241176B (https=)
MX (2) MX366647B (https=)
SG (1) SG11201506034TA (https=)
TW (1) TWI636136B (https=)
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Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10202630B2 (en) * 2013-03-15 2019-02-12 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
PE20161439A1 (es) 2014-03-21 2017-01-26 Teva Pharmaceuticals Int Gmbh Anticuerpos antagonistas dirigidos contra el peptido relacionado con el gen de calcitonina y metodos que usan los mismos
US10556945B2 (en) 2014-03-21 2020-02-11 Teva Pharmaceuticals International Gmbh Antagonist antibodies directed against calcitonin gene-related peptide and methods using same
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
JP2017045865A (ja) 2015-08-26 2017-03-02 ルネサスエレクトロニクス株式会社 半導体装置およびその製造方法
CN106191181B (zh) * 2016-07-22 2019-12-13 江苏江山聚源生物技术有限公司 一种毕赤酵母表达重组蛋白的发酵工艺
WO2018055574A1 (en) 2016-09-23 2018-03-29 Teva Pharmaceuticals International Gmbh Treating refractory migraine
EP3592800A4 (en) 2017-03-10 2021-01-06 Bolt Threads, Inc. COMPOSITIONS AND METHODS OF PRODUCING HIGHLY SECRETED YIELD OF RECOMBINANT PROTEIN
KR20250121449A (ko) 2017-03-10 2025-08-12 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
IL277352B2 (en) * 2018-03-16 2025-08-01 Genzyme Corp Methods for improving cell viability in a production bioreactor
US10221403B1 (en) * 2018-04-25 2019-03-05 Life Rainbow Biotech Co., Ltd. Method of preparing zearalenone hydrolase
CN108546637A (zh) * 2018-05-23 2018-09-18 华东理工大学 重组毕赤酵母表达葡萄糖氧化酶发酵装置以及发酵培养方法
MX2021000053A (es) * 2018-07-12 2021-03-25 Amyris Inc Metodos para controlar velocidades de alimentacion de la fermentacion.
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
FI4055177T3 (fi) * 2019-11-08 2024-10-31 Danisco Us Inc Parantuneen proteiinituottavuuden fenotyyppejä käsittäviä sienikantoja ja menetelmiä niitä varten
WO2021146233A1 (en) 2020-01-14 2021-07-22 Basf Se Method for producing recombinant protein in yeast cells
US20250207158A1 (en) * 2022-03-24 2025-06-26 Versalis S.P.A. Fermentation process for the production of lipids from oleaginous yeasts
EP4594342A2 (en) * 2022-09-29 2025-08-06 Antheia, Inc. Methods of improving production of morphinan alkaloids and derivatives

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221630B1 (en) 1999-03-24 2001-04-24 The Penn State Research Foundation High copy number recombinant expression construct for regulated high-level production of polypeptides in yeast
WO2013028635A1 (en) * 2011-08-19 2013-02-28 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
WO1994021293A1 (en) 1993-03-19 1994-09-29 Duke University Method of treatment of tumors with an antibody binding to tenascin
ES2251723T3 (es) 1994-08-12 2006-05-01 Immunomedics, Inc. Inmunoconjugados y anticuerpos humanizados especificos para linfoma de celulas b y celulas de leucemia.
US5955349A (en) 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5736383A (en) 1996-08-26 1998-04-07 Zymogenetics, Inc. Preparation of Pichia methanolica auxotrophic mutants
US6730499B1 (en) 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
PT1678314E (pt) 2003-10-22 2012-11-27 Keck Graduate Inst Métodos de síntese de polipéptidos heteromultiméricos em levedura utilizando uma estratégia de conjugação haplóide
DK2021463T3 (en) 2006-05-19 2017-01-16 Alder Biopharmaceuticals Inc Culture method to obtain a cloned population of antigen-specific B cells
HUE032563T2 (en) 2007-05-21 2017-09-28 Alderbio Holdings Llc Antibodies to IL-6 and their use
KR20170036814A (ko) 2007-05-21 2017-04-03 앨더바이오 홀딩스 엘엘씨 신규한 래빗 항체 인간화 방법 및 인간화된 래빗 항체
US20090269832A1 (en) 2007-11-05 2009-10-29 Modular Genetics, Inc. Growth of Microorganisms in Media Containing Crude Glycerol
US8501449B2 (en) 2007-12-04 2013-08-06 Proteon Therapeutics, Inc. Recombinant elastase proteins and methods of manufacturing and use thereof
US8609416B2 (en) 2009-12-18 2013-12-17 Ventria Bioscience Methods and compositions comprising heat shock proteins
US11214610B2 (en) 2010-12-01 2022-01-04 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
US10150968B2 (en) 2011-08-19 2018-12-11 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
US10202630B2 (en) * 2013-03-15 2019-02-12 Alderbio Holdings Llc Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
US10189892B2 (en) * 2013-03-15 2019-01-29 Alderbio Holdings Llc Fermentation process for antibody production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221630B1 (en) 1999-03-24 2001-04-24 The Penn State Research Foundation High copy number recombinant expression construct for regulated high-level production of polypeptides in yeast
WO2013028635A1 (en) * 2011-08-19 2013-02-28 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Proc Natl Acad Sci USA,제76권,제10호,5222-5225면(1979) 1부.*
논문2:Protein Expr Purif. 2003

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TWI636136B (zh) 2018-09-21
TW201512401A (zh) 2015-04-01
WO2014145650A1 (en) 2014-09-18
EP3597664A2 (en) 2020-01-22
ES3057435T3 (en) 2026-03-02
EP2971037A1 (en) 2016-01-20
US10138294B2 (en) 2018-11-27
US11447540B2 (en) 2022-09-20
US20160039911A1 (en) 2016-02-11
MX366647B (es) 2019-07-17
EP3597664C0 (en) 2025-11-26
EP2971037B1 (en) 2019-07-10
JP2016516412A (ja) 2016-06-09
SG11201506034TA (en) 2015-09-29
EP3597664B1 (en) 2025-11-26
IL241176A0 (en) 2015-11-30
MX2015011968A (es) 2017-01-06
CA2905180A1 (en) 2014-09-18
MX384491B (es) 2025-03-14
US20190119360A1 (en) 2019-04-25
AU2014232813B2 (en) 2017-12-07
AU2014232813A1 (en) 2015-09-03
CN105121652A (zh) 2015-12-02
NZ711400A (en) 2021-05-28
CA2905180C (en) 2023-03-07
DK2971037T3 (da) 2019-10-14
ES2749751T3 (es) 2020-03-23
EP2971037A4 (en) 2016-07-20
EP3597664A3 (en) 2020-03-11
MX2019008417A (es) 2019-09-13
KR20150140678A (ko) 2015-12-16
ZA201505483B (en) 2022-08-31
CN105121652B (zh) 2020-07-28
IL241176B (en) 2020-05-31
JP6412552B2 (ja) 2018-10-24

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