WO2013042960A3 - 미세소낭성 adam15의 제조방법 - Google Patents

미세소낭성 adam15의 제조방법 Download PDF

Info

Publication number
WO2013042960A3
WO2013042960A3 PCT/KR2012/007549 KR2012007549W WO2013042960A3 WO 2013042960 A3 WO2013042960 A3 WO 2013042960A3 KR 2012007549 W KR2012007549 W KR 2012007549W WO 2013042960 A3 WO2013042960 A3 WO 2013042960A3
Authority
WO
WIPO (PCT)
Prior art keywords
adam15
microvesicular
preparing
present
mammalian cells
Prior art date
Application number
PCT/KR2012/007549
Other languages
English (en)
French (fr)
Other versions
WO2013042960A2 (ko
Inventor
김두식
이희두
김연향
구본훈
전옥희
Original Assignee
연세대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 연세대학교 산학협력단 filed Critical 연세대학교 산학협력단
Priority to US14/125,622 priority Critical patent/US20140212949A1/en
Publication of WO2013042960A2 publication Critical patent/WO2013042960A2/ko
Publication of WO2013042960A3 publication Critical patent/WO2013042960A3/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
    • A61K31/23—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin of acids having a carboxyl group bound to a chain of seven or more carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11013—Protein kinase C (2.7.11.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Epidemiology (AREA)
  • Emergency Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 (a) 분리된 포유동물 세포의 PKC(Protein Kinase C)를 활성화시키는 단계; 및 (b) 상기 포유동물 세포로부터 ADAM15를 포함하는 미세소낭(exosome)을 분리하는 단계를 포함하는 미세소낭성 ADAM15의 제조방법에 관한 것이다. 본 발명에 따르면, 포유동물의 세포로부터 미세소낭성 ADAM15의 제조방법을 제공할 수 있으며, 미세소낭성 ADAM15의 기능 연구를 통해 ADAM 단백질에 의해 매개되는 새로운 세포 조절기작을 제공할 수 있다. 또한, 본 발명은 종양세포의 부착, 증식 및 이동을 억제하는 미세소낭성 ADAM15를 이용한 새로운 항암제를 제공할 수 있다.
PCT/KR2012/007549 2011-09-20 2012-09-20 미세소낭성 adam15의 제조방법 WO2013042960A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/125,622 US20140212949A1 (en) 2011-09-20 2012-09-20 Method for preparing microvesicular adam15

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0094376 2011-09-20
KR1020110094376A KR101347190B1 (ko) 2011-09-20 2011-09-20 미세소낭성 ａｄａｍ１５의 제조 방법

Publications (2)

Publication Number Publication Date
WO2013042960A2 WO2013042960A2 (ko) 2013-03-28
WO2013042960A3 true WO2013042960A3 (ko) 2013-05-23

Family

ID=47915015

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/007549 WO2013042960A2 (ko) 2011-09-20 2012-09-20 미세소낭성 adam15의 제조방법

Country Status (3)

Country Link
US (1) US20140212949A1 (ko)
KR (1) KR101347190B1 (ko)
WO (1) WO2013042960A2 (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101473557B1 (ko) * 2013-03-29 2014-12-24 포항공과대학교 산학협력단 원심력을 이용한 세포유래 인공 마이크로베시클 제조 장치
RU2710373C2 (ru) 2014-11-07 2019-12-26 Экзостемтех Ко., Лтд. Композиция, включающая экзосому, полученную из стволовых клеток, для индукции адипогенной дифференцировки, регенерации жировой ткани, отбеливания кожи или коррекции морщин
KR101629151B1 (ko) 2014-11-07 2016-06-10 한양대학교 에리카산학협력단 줄기세포 유래 엑소좀을 포함하는 지방세포 분화유도 및 지방조직 재생용 조성물
US20180325830A1 (en) * 2017-05-09 2018-11-15 Vivex Biomedical, Inc. Coated biological composition
CN114848653B (zh) * 2021-02-03 2023-05-30 四川大学 RORa蛋白及其激动剂在制备急性DNA损伤修复剂中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100802140B1 (ko) * 2007-02-07 2008-02-11 한국생명공학연구원 돼지 유래 신규 ａｄａｍ３ 유전자 및 이를 이용한 돼지정자의 수정능 판별방법
KR20090009538A (ko) * 2007-07-20 2009-01-23 숙명여자대학교산학협력단 인터류킨―１８의 분비를 조절하는 ａｄａｍ３３ 및 이의용도

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100802140B1 (ko) * 2007-02-07 2008-02-11 한국생명공학연구원 돼지 유래 신규 ａｄａｍ３ 유전자 및 이를 이용한 돼지정자의 수정능 판별방법
KR20090009538A (ko) * 2007-07-20 2009-01-23 숙명여자대학교산학협력단 인터류킨―１８의 분비를 조절하는 ａｄａｍ３３ 및 이의용도

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
F. H. JANSEN ET AL.: "Exosomal secretion of cytoplasmic prostate cancer xenograft-derived proteins", MOL CELL PROTEOMICS., vol. 8, no. 6, June 2009 (2009-06-01), pages 1192 - 1205 *
S.H. CHOI ET AL.: "Differential effect ofbryostatin 1 and phorbol 12-myristate 13-acetate on HOP-92 cell proliferation is mediated by down-regulation of protein kinase Cdelta", CANCER RES., vol. 66, no. 14, 15 July 2006 (2006-07-15), pages 7261 - 7269 *

Also Published As

Publication number Publication date
US20140212949A1 (en) 2014-07-31
KR20130030846A (ko) 2013-03-28
KR101347190B1 (ko) 2014-01-07
WO2013042960A2 (ko) 2013-03-28

Similar Documents

Publication Publication Date Title
WO2013042960A3 (ko) 미세소낭성 adam15의 제조방법
WO2014028453A3 (en) Natural killer cells and uses thereof
WO2013060867A3 (en) Production of heterodimeric proteins
NZ630568A (en) Methods and means for the production of ch3 domain-comprising molecules
WO2011120690A3 (en) Separation of insoluble target proteins
WO2014035475A8 (en) Methods to control protein heterogeneity
MX359384B (es) Conjunto mejorado de anticuerpos bisespecificos.
CA2848209C (en) T cell receptors recognizing hla-a1- or hla-cw7-restricted mage
WO2009114702A3 (en) Recombinant production of authentic human proteins using human cell expression systems
EP2649178B8 (en) Mgmt-based method for obtaining high yield of recombinant protein expression
WO2012068317A3 (en) Methods for producing recombinant proteins
SG10201810830UA (en) Cell culture compositions and methods for polypeptide production
MX2019005999A (es) Precipitacion de la fraccion i-iv-1 de inmunoglobulinas plasmaticas.
WO2012140127A3 (en) Method for priming of t cells
SG10201809452RA (en) Cell culture compositions with antioxidants and methods for polypeptide production
WO2012145759A3 (en) Methods of protein production and compositions thereof
WO2012165854A3 (ko) 표적 유전자 발현 억제 및 면역 반응을 동시에 유발하는 이중가닥의 긴 간섭 ｒｎａ
WO2010023310A3 (en) The an3 protein complex and its use for plant growth promotion
AU2012312028A8 (en) Detection of isotype profiles as signatures for disease
WO2013032174A3 (ko) 단백질 합성 키트，자동추출장비를 이용한 단백질 발현 및 추출 방법
EP3527667A3 (en) Yeast promoters for protein expression
WO2013021362A3 (fr) Nouveau procédé de déstabilisation d'une émulsion de pickering
EP3162892A4 (en) Method for culturing adhesive cells, culture vessel, and method for producing protein
MX2021001418A (es) Produccion de proteinas heteromultimericas usando celulas de mamifero.
EA200900984A1 (ru) Улучшение клеточного роста

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12834490

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 14125622

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12834490

Country of ref document: EP

Kind code of ref document: A2