WO2013032174A3 - 단백질 합성 키트，자동추출장비를 이용한 단백질 발현 및 추출 방법 - Google Patents

단백질 합성 키트，자동추출장비를 이용한 단백질 발현 및 추출 방법 Download PDF

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Publication number
WO2013032174A3
WO2013032174A3 PCT/KR2012/006715 KR2012006715W WO2013032174A3 WO 2013032174 A3 WO2013032174 A3 WO 2013032174A3 KR 2012006715 W KR2012006715 W KR 2012006715W WO 2013032174 A3 WO2013032174 A3 WO 2013032174A3
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WIPO (PCT)
Prior art keywords
proteins
expressing
protein synthesis
extraction equipment
synthesis kit
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PCT/KR2012/006715
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English (en)
French (fr)
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WO2013032174A2 (ko
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박한오
조유상
정준호
한지원
김남일
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(주)바이오니아
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Priority to JP2014527074A priority Critical patent/JP2014524262A/ja
Priority to EP12828394.2A priority patent/EP2749567A4/en
Priority to CN201280041689.1A priority patent/CN103827137A/zh
Priority to US14/238,908 priority patent/US9163272B2/en
Priority to CA2845598A priority patent/CA2845598A1/en
Priority to AU2012302478A priority patent/AU2012302478A1/en
Priority to BR112014004543A priority patent/BR112014004543A2/pt
Publication of WO2013032174A2 publication Critical patent/WO2013032174A2/ko
Publication of WO2013032174A3 publication Critical patent/WO2013032174A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C12M1/18—Multiple fields or compartments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 단백질의 생산방법에 관한 것으로，상기 본 발명에 따른 단백질 생산방법은 본 발명의 멀티 웰 플레이트 키트 및 히팅부와 자기장 인가부를 구비한 생물학적 물질 자동정제 장치를 이용함으로써 기존의 종래의 세포배양을 통한 단백질 발현 / 추출 방법을 사용하여 목적 단백질을 얻는 경우에 비해 다수의 단백질을 신속하고 간편하게 획득할 수 있으며，반응 웰 사이의 편차가 없어 동일 단백질에 대해서 재현성 있는 합성 효율을 얻을 수 있다는 장점이 있다.
PCT/KR2012/006715 2011-08-26 2012-08-23 단백질 합성 키트，자동추출장비를 이용한 단백질 발현 및 추출 방법 WO2013032174A2 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2014527074A JP2014524262A (ja) 2011-08-26 2012-08-23 タンパク質合成キット、自動抽出装置を用いたタンパク質発現および抽出方法
EP12828394.2A EP2749567A4 (en) 2011-08-26 2012-08-23 PROTEIN SYNTHESIS KIT AND METHOD FOR THE EXPRESSION AND EXTRACTION OF PROTEINS WITH AN AUTOMATIC EXTRACTION DEVICE
CN201280041689.1A CN103827137A (zh) 2011-08-26 2012-08-23 蛋白质合成试剂盒以及使用自动纯化设备表达和纯化蛋白质的方法
US14/238,908 US9163272B2 (en) 2011-08-26 2012-08-23 Protein synthesis kit, and method for expressing and extracting proteins using automatic extraction equipment
CA2845598A CA2845598A1 (en) 2011-08-26 2012-08-23 Protein synthesis kit, and method for protein expression and purification using automatic purification equipment
AU2012302478A AU2012302478A1 (en) 2011-08-26 2012-08-23 Protein synthesis kit, and method for protein expression and purification using automatic purification equipment
BR112014004543A BR112014004543A2 (pt) 2011-08-26 2012-08-23 método de síntese de proteínas

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20110085824 2011-08-26
KR10-2011-0085824 2011-08-26
KR10-2012-0090149 2012-08-17
KR1020120090149A KR20130023091A (ko) 2011-08-26 2012-08-17 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법

Publications (2)

Publication Number Publication Date
WO2013032174A2 WO2013032174A2 (ko) 2013-03-07
WO2013032174A3 true WO2013032174A3 (ko) 2013-04-25

Family

ID=48175583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/006715 WO2013032174A2 (ko) 2011-08-26 2012-08-23 단백질 합성 키트，자동추출장비를 이용한 단백질 발현 및 추출 방법

Country Status (9)

Country Link
US (1) US9163272B2 (ko)
EP (1) EP2749567A4 (ko)
JP (1) JP2014524262A (ko)
KR (1) KR20130023091A (ko)
CN (1) CN103827137A (ko)
AU (1) AU2012302478A1 (ko)
BR (1) BR112014004543A2 (ko)
CA (1) CA2845598A1 (ko)
WO (1) WO2013032174A2 (ko)

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Publication number Priority date Publication date Assignee Title
US9982227B2 (en) 2011-03-08 2018-05-29 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
US9388373B2 (en) 2011-03-08 2016-07-12 University Of Maryland Baltimore County Microscale bioprocessing system and method for protein manufacturing
US10774304B2 (en) 2011-03-08 2020-09-15 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
EP2927312B1 (en) * 2012-11-30 2022-10-19 Bioneer Corporation Apparatus for automatically preparing cell-free proteins and method for preparing proteins using the same
KR101644906B1 (ko) * 2016-05-23 2016-08-02 충남대학교산학협력단 단일 용기 내에서 합성된 전사체 또는 단백질과 타겟 세포와의 상호작용을 분석하는 방법
CN107987120A (zh) * 2017-12-27 2018-05-04 湖北普罗金科技有限公司 一种适宜于重力柱的蛋白质纯化自动装置
KR102256776B1 (ko) * 2018-07-26 2021-05-27 (주)바이오니아 자석봉 블록의 교체가 가능한 표적물질 추출장치
CN110283716B (zh) * 2019-06-20 2020-11-06 清华大学 一种用于无细胞蛋白质连续合成的装置和方法
CN111349546B (zh) * 2020-03-18 2020-09-15 江苏支点生物科技有限公司 一种无细胞蛋白质合成方法及多孔板
KR20220075978A (ko) 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법
CN115389402B (zh) * 2022-10-26 2023-03-24 安徽佰欧晶医学科技有限公司 一种血液肿瘤测定试剂盒以及检测方法

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JPH0731494A (ja) * 1993-07-14 1995-02-03 Hitachi Ltd 無細胞系におけるタンパク質の生産方法及び装置
JP2003009877A (ja) * 2001-07-02 2003-01-14 Inst Of Physical & Chemical Res 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
WO2008010687A1 (en) * 2006-07-20 2008-01-24 Seoul National University Industry Foundation Method for selective binding, separation or purification of proteins using magnetic nanoparticles
KR101025135B1 (ko) * 2008-04-09 2011-03-31 (주)바이오니아 자동정제장치, 멀티 웰 플레이트 키트 및 생물학적 시료로부터 핵산을 추출하는 방법

Also Published As

Publication number Publication date
AU2012302478A1 (en) 2014-03-06
CN103827137A (zh) 2014-05-28
EP2749567A2 (en) 2014-07-02
KR20130023091A (ko) 2013-03-07
EP2749567A4 (en) 2015-04-01
US9163272B2 (en) 2015-10-20
WO2013032174A2 (ko) 2013-03-07
CA2845598A1 (en) 2013-03-07
US20140212919A1 (en) 2014-07-31
BR112014004543A2 (pt) 2017-04-04
JP2014524262A (ja) 2014-09-22

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