WO2013019075A3 - 핵산분자의 제조방법 - Google Patents

핵산분자의 제조방법 Download PDF

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WO2013019075A3
WO2013019075A3 PCT/KR2012/006147 KR2012006147W WO2013019075A3 WO 2013019075 A3 WO2013019075 A3 WO 2013019075A3 KR 2012006147 W KR2012006147 W KR 2012006147W WO 2013019075 A3 WO2013019075 A3 WO 2013019075A3
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nucleic acid
acid fragments
acid molecules
preparing nucleic
sequence
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PCT/KR2012/006147
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French (fr)
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WO2013019075A2 (ko
WO2013019075A9 (ko
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방두희
김황범
한효준
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연세대학교산학협력단
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Priority to US14/235,799 priority Critical patent/US9340826B2/en
Publication of WO2013019075A2 publication Critical patent/WO2013019075A2/ko
Publication of WO2013019075A9 publication Critical patent/WO2013019075A9/ko
Publication of WO2013019075A3 publication Critical patent/WO2013019075A3/ko
Priority to US15/132,245 priority patent/US10358642B2/en

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

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  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

(a) 목적 핵산분자의 전체 서열의 적어도 일부를 구성하는 핵산 단편들을 제공하는 단계; (b) 상기 핵산 단편들을 바코드 서열들(barcode sequences)로 태깅(tagging)하는 단계; (c) 상기 바코드 서열들로 태깅된 핵산 단편들의 서열을 확인하는 단계; 및 (d) 상기 서열 확인된 핵산 단편들 중 소망하는 핵산 단편들을 상기 바코드 서열들을 이용하여 회수하는 단계를 포함하는 핵산분자의 제조방법이 제공된다.
PCT/KR2012/006147 2011-08-01 2012-08-01 핵산분자의 제조방법 WO2013019075A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US14/235,799 US9340826B2 (en) 2011-08-01 2012-08-01 Method of preparing nucleic acid molecules
US15/132,245 US10358642B2 (en) 2011-08-01 2016-04-19 Method of preparing nucleic acid molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20110076408 2011-08-01
KR10-2011-0076408 2011-08-01

Related Child Applications (2)

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US14/235,799 A-371-Of-International US9340826B2 (en) 2011-08-01 2012-08-01 Method of preparing nucleic acid molecules
US15/132,245 Continuation US10358642B2 (en) 2011-08-01 2016-04-19 Method of preparing nucleic acid molecules

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WO2013019075A2 WO2013019075A2 (ko) 2013-02-07
WO2013019075A9 WO2013019075A9 (ko) 2013-05-02
WO2013019075A3 true WO2013019075A3 (ko) 2013-07-04

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US (2) US9340826B2 (ko)
KR (2) KR101454886B1 (ko)
WO (1) WO2013019075A2 (ko)

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US9340826B2 (en) 2016-05-17
US10358642B2 (en) 2019-07-23
KR20130018575A (ko) 2013-02-25
WO2013019075A2 (ko) 2013-02-07
US20160222380A1 (en) 2016-08-04
KR20140004053A (ko) 2014-01-10
US20140309118A1 (en) 2014-10-16
KR101467969B1 (ko) 2014-12-02
KR101454886B1 (ko) 2014-11-03
WO2013019075A9 (ko) 2013-05-02

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