BR112014004543A2 - método de síntese de proteínas - Google Patents

método de síntese de proteínas

Info

Publication number
BR112014004543A2
BR112014004543A2 BR112014004543A BR112014004543A BR112014004543A2 BR 112014004543 A2 BR112014004543 A2 BR 112014004543A2 BR 112014004543 A BR112014004543 A BR 112014004543A BR 112014004543 A BR112014004543 A BR 112014004543A BR 112014004543 A2 BR112014004543 A2 BR 112014004543A2
Authority
BR
Brazil
Prior art keywords
protein synthesis
synthesis method
proteins
target proteins
express
Prior art date
Application number
BR112014004543A
Other languages
English (en)
Inventor
Oh Park Han
Won Han Ji
Ho Jung Jun
Il Kim Nam
Sang Cho You
Original Assignee
Bioneer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioneer Corp filed Critical Bioneer Corp
Publication of BR112014004543A2 publication Critical patent/BR112014004543A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/16—Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C12M1/18—Multiple fields or compartments
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00457—Dispensing or evacuation of the solid phase support
    • B01J2219/00459—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00495—Means for heating or cooling the reaction vessels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

resumo método de síntese de proteínas é proporcionado um método de síntese de proteínas. o método de síntese de proteínas de acordo com a presente invenção utiliza um aparelho automático de purificação de material biológico incluindo: um kit de placa com poço, uma parte de aquecimento, e uma parte de aplicação de campo magnético, de modo que uma pluralidade de proteínas alvo pode ser mais rápida e simplesmente obtida quando comparado com proteínas alvo obtidas usando o método existente para expressar/purificar proteínas por meio de cultura de célula convencional, e uma eficiência de síntese reprodutível nas mesmas proteínas pode ser obtida devido a nenhum desvio entre os poços de reação.
BR112014004543A 2011-08-26 2012-08-23 método de síntese de proteínas BR112014004543A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20110085824 2011-08-26
KR1020120090149A KR20130023091A (ko) 2011-08-26 2012-08-17 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법
PCT/KR2012/006715 WO2013032174A2 (ko) 2011-08-26 2012-08-23 단백질 합성 키트，자동추출장비를 이용한 단백질 발현 및 추출 방법

Publications (1)

Publication Number Publication Date
BR112014004543A2 true BR112014004543A2 (pt) 2017-04-04

Family

ID=48175583

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014004543A BR112014004543A2 (pt) 2011-08-26 2012-08-23 método de síntese de proteínas

Country Status (9)

Country Link
US (1) US9163272B2 (pt)
EP (1) EP2749567A4 (pt)
JP (1) JP2014524262A (pt)
KR (1) KR20130023091A (pt)
CN (1) CN103827137A (pt)
AU (1) AU2012302478A1 (pt)
BR (1) BR112014004543A2 (pt)
CA (1) CA2845598A1 (pt)
WO (1) WO2013032174A2 (pt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012122413A1 (en) 2011-03-08 2012-09-13 University Of Maryland Baltimore County Microscale bioprocessing system and method for protein manufacturing
US10774304B2 (en) 2011-03-08 2020-09-15 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
US9982227B2 (en) 2011-03-08 2018-05-29 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
RU2615446C2 (ru) * 2012-11-30 2017-04-04 Байонир Корпорейшн Прибор для автоматизированного бесклеточного получения белков и способ получения белков с применением данного прибора
KR101644906B1 (ko) * 2016-05-23 2016-08-02 충남대학교산학협력단 단일 용기 내에서 합성된 전사체 또는 단백질과 타겟 세포와의 상호작용을 분석하는 방법
CN107987120A (zh) * 2017-12-27 2018-05-04 湖北普罗金科技有限公司 一种适宜于重力柱的蛋白质纯化自动装置
KR102256776B1 (ko) * 2018-07-26 2021-05-27 (주)바이오니아 자석봉 블록의 교체가 가능한 표적물질 추출장치
CN110283716B (zh) * 2019-06-20 2020-11-06 清华大学 一种用于无细胞蛋白质连续合成的装置和方法
CN111349546B (zh) * 2020-03-18 2020-09-15 江苏支点生物科技有限公司 一种无细胞蛋白质合成方法及多孔板
KR20220075978A (ko) 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법
CN115389402B (zh) * 2022-10-26 2023-03-24 安徽佰欧晶医学科技有限公司 一种血液肿瘤测定试剂盒以及检测方法

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JPH0731494A (ja) 1993-07-14 1995-02-03 Hitachi Ltd 無細胞系におけるタンパク質の生産方法及び装置
EP1801214B1 (en) * 1997-07-07 2010-11-10 Medical Research Council In vitro sorting method
WO1999011777A1 (en) * 1997-09-03 1999-03-11 Biovation Limited Methods for protein screening
US6927025B1 (en) * 1997-09-03 2005-08-09 Biovation Limited Methods for protein screening
JP2003009877A (ja) 2001-07-02 2003-01-14 Inst Of Physical & Chemical Res 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
JP4768155B2 (ja) 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
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KR20080008668A (ko) 2006-07-20 2008-01-24 재단법인서울대학교산학협력재단 자성체 나노입자를 이용하여 단백질을 선택적으로 결합,분리 또는 정제하는 방법
CN101363045B (zh) * 2007-09-10 2012-10-17 厦门致善生物科技有限公司 一种用超微磁颗粒分离纯化生物靶物质的方法
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JP2011516075A (ja) * 2008-04-09 2011-05-26 バイオニア コーポレーション 自動精製装置、マルチウェルプレートキット及び生物学的試料からヘキサンを抽出する方法
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Also Published As

Publication number Publication date
EP2749567A2 (en) 2014-07-02
US20140212919A1 (en) 2014-07-31
KR20130023091A (ko) 2013-03-07
JP2014524262A (ja) 2014-09-22
EP2749567A4 (en) 2015-04-01
US9163272B2 (en) 2015-10-20
AU2012302478A1 (en) 2014-03-06
WO2013032174A3 (ko) 2013-04-25
CN103827137A (zh) 2014-05-28
WO2013032174A2 (ko) 2013-03-07
CA2845598A1 (en) 2013-03-07

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]