JP2015517310A5 - - Google Patents

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JP2015517310A5
JP2015517310A5 JP2015511775A JP2015511775A JP2015517310A5 JP 2015517310 A5 JP2015517310 A5 JP 2015517310A5 JP 2015511775 A JP2015511775 A JP 2015511775A JP 2015511775 A JP2015511775 A JP 2015511775A JP 2015517310 A5 JP2015517310 A5 JP 2015517310A5
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polypeptide
host cell
seq
amino acid
position corresponding
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JP2015511775A
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JP2015517310A (ja
JP6321631B2 (ja
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Priority claimed from PCT/US2013/040627 external-priority patent/WO2013176909A2/en
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JP2015511775A 2012-05-11 2013-05-10 ケトール酸レダクトイソメラーゼ酵素及び使用方法 Active JP6321631B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261645832P 2012-05-11 2012-05-11
US61/645,832 2012-05-11
US201361787480P 2013-03-15 2013-03-15
US61/787,480 2013-03-15
PCT/US2013/040627 WO2013176909A2 (en) 2012-05-11 2013-05-10 Ketol-acid reductoisomerase enzymes and methods of use

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JP2015517310A JP2015517310A (ja) 2015-06-22
JP2015517310A5 true JP2015517310A5 (enExample) 2016-06-23
JP6321631B2 JP6321631B2 (ja) 2018-05-09

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US (2) US9169467B2 (enExample)
EP (1) EP2847326A2 (enExample)
JP (1) JP6321631B2 (enExample)
KR (1) KR20150014953A (enExample)
CN (1) CN104619835B (enExample)
AU (1) AU2013266727A1 (enExample)
BR (1) BR112014028153A2 (enExample)
CA (1) CA2873109A1 (enExample)
MX (2) MX370134B (enExample)
NZ (1) NZ701099A (enExample)
WO (1) WO2013176909A2 (enExample)
ZA (1) ZA201407713B (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
WO2008130995A2 (en) 2007-04-18 2008-10-30 E. I. Du Pont De Nemours And Company Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
AU2009296224B2 (en) 2008-09-29 2015-02-12 Butamax(Tm) Advanced Biofuels Llc Increased heterologous Fe-S enzyme actiivty in yeast
EP2519628A2 (en) 2009-12-29 2012-11-07 Butamax (TM) Advanced Biofuels LLC Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
KR20130027063A (ko) 2010-02-17 2013-03-14 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 Ｆｅ-ｓ 클러스터 요구성 단백질의 활성 향상
CA2810244C (en) 2010-09-07 2020-10-06 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
US9790521B2 (en) 2011-03-24 2017-10-17 Butamax Advanced Biofuels Llc Host cells and methods for production of isobutanol
CN105473726B (zh) 2011-07-28 2021-03-12 布特马斯先进生物燃料有限责任公司 酮异戊酸脱羧酶及其使用方法
JP2015503350A (ja) 2011-12-30 2015-02-02 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー アルコールの発酵生産
AU2013235391A1 (en) 2012-03-23 2014-09-04 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
CA2873109A1 (en) 2012-05-11 2013-11-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
CA2881407A1 (en) 2012-08-22 2014-02-27 Butamax Advanced Biofuels Llc Production of fermentation products
US9840724B2 (en) 2012-09-21 2017-12-12 Butamax Advanced Biofuels Llc Production of renewable hydrocarbon compositions
BR112015006691A2 (pt) 2012-09-26 2017-12-12 Butamax Advanced Biofuels Llc métodos, célula hospedeira recombinante, composição, polipeptídeo, polinucleotídeos e vetor recombinante
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
WO2014106107A2 (en) 2012-12-28 2014-07-03 Butamax (Tm) Advanced Biofuels Llc Dhad variants for butanol production
CN105378057A (zh) 2013-03-14 2016-03-02 纳幕尔杜邦公司 用于丁醇生产的甘油3-磷酸脱氢酶
US9771602B2 (en) 2013-03-15 2017-09-26 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening
US10280438B2 (en) 2014-08-11 2019-05-07 Butamax Advanced Biofuels Llc Method for the production of yeast
US20190276905A1 (en) 2016-11-09 2019-09-12 Danisco Us Inc. Yeast with improved alcohol production
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
KR101960501B1 (ko) * 2017-09-15 2019-03-21 고려대학교 산학협력단 Ｎａｄｈ 친화적인 신규 케톨산 리덕토아이소머라제
US11308274B2 (en) 2019-05-17 2022-04-19 International Business Machines Corporation Word grouping using a plurality of models
CN113278568B (zh) * 2020-05-27 2022-10-21 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组大肠杆菌及其应用
KR102801647B1 (ko) * 2022-08-12 2025-04-30 씨제이제일제당 주식회사 케톨산 리덕토아이소머라제 변이체 및 이를 이용한 l-발린 생산 방법
WO2024197704A1 (zh) * 2023-03-30 2024-10-03 安徽华恒生物科技股份有限公司 一种产泛解酸的重组微生物及其应用
CN117417949B (zh) * 2023-10-19 2024-12-20 云南农业大学 雪胆细胞色素氧化酶HcCYP81Q58转基因酵母工程菌及其应用

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4865973A (en) 1985-09-13 1989-09-12 Queen's University At Kingston Process for extractive fermentation
FR2696190B1 (fr) 1992-09-25 1994-12-09 Agronomique Inst Nat Rech Acide nucléique codant pour une alpha-acétolactate synthase et ses applications.
EP1204755B1 (en) 1999-08-18 2006-08-09 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol
KR20020097200A (ko) 2000-03-21 2002-12-31 엘리트라 파마슈티컬즈, 인코포레이티드 원핵세포에서의 필수유전자의 동정
US6586229B1 (en) 2000-06-01 2003-07-01 North Carolina State University Method for the production of ρ-Hydroxybenzoate in species of pseudomonas and agrobacterium
CA2450867A1 (en) 2001-07-02 2003-01-16 Kaneka Corporation Method of modifying enzyme and oxidoreductase variant
MXPA05009553A (es) 2003-03-10 2006-03-17 Broin And Associates Inc Metodo para producir etanol utilizando almidon como materia prima.
SE526429C2 (sv) 2003-10-24 2005-09-13 Swedish Biofuels Ab Metod för att framställa syreinnehållande föreningar utgående från biomassa
JP4991700B2 (ja) 2005-04-12 2012-08-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー バイオマス処理のためのシステムおよび方法
US9297028B2 (en) 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US8956850B2 (en) 2008-06-05 2015-02-17 Butamax Advanced Biofuels Llc Enhanced pyruvate to acetolactate conversion in yeast
US8273558B2 (en) * 2005-10-26 2012-09-25 Butamax(Tm) Advanced Biofuels Llc Fermentive production of four carbon alcohols
EP3301182B1 (en) * 2005-10-26 2020-03-18 Butamax (TM) Advanced Biofuels LLC Fermentative production of four carbon alcohols
US8945899B2 (en) 2007-12-20 2015-02-03 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase using NADH
US9303225B2 (en) 2005-10-26 2016-04-05 Butamax Advanced Biofuels Llc Method for the production of isobutanol by recombinant yeast
US8962298B2 (en) 2006-05-02 2015-02-24 Butamax Advanced Biofuels Llc Recombinant host cell comprising a diol dehydratase
US8828704B2 (en) 2006-05-02 2014-09-09 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US7659104B2 (en) 2006-05-05 2010-02-09 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US7541173B2 (en) 2006-06-15 2009-06-02 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
US8017364B2 (en) 2006-12-12 2011-09-13 Butamax(Tm) Advanced Biofuels Llc Solvent tolerant microorganisms
WO2008098227A2 (en) 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms
WO2008130995A2 (en) 2007-04-18 2008-10-30 E. I. Du Pont De Nemours And Company Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
EP2060632A1 (en) 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol
US8431374B2 (en) 2007-10-31 2013-04-30 Gevo, Inc. Methods for the economical production of biofuel from biomass
DE602007011966D1 (de) 2007-12-17 2011-02-24 Suntory Holdings Ltd Mutantes ilv5-gen und verwendung davon
CA2709372A1 (en) 2007-12-20 2009-07-09 Butamaxtm Advanced Biofuels Llc Ketol-acid reductoisomerase using nadh
US8372612B2 (en) 2007-12-21 2013-02-12 Butamax(Tm) Advanced Biofuels Llc Production of four carbon alcohols using improved strain
US8518678B2 (en) 2007-12-21 2013-08-27 Butamax(Tm) Advanced Biofuels Llc Strain comprising increased expression of a CFA coding region for butanol production
CA2710359C (en) 2007-12-23 2018-02-20 Gevo, Inc. Yeast organism producing isobutanol at a high yield
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
US8389252B2 (en) 2008-05-12 2013-03-05 Butamax(Tm) Advanced Biofuels Llc Yeast strain for production of four carbon alcohols
US8906666B2 (en) 2008-05-22 2014-12-09 Butamax Advanced Biofuels Llc Engineering resistance to aliphatic alcohols
BRPI0913681A2 (pt) 2008-09-29 2019-09-24 Butamax Advanced Biofuels Llc "célula bacteriana ácido-lática recombinante e método de produção de isobutanol"
US20100081154A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES
AU2009296232A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria
AU2009296224B2 (en) 2008-09-29 2015-02-12 Butamax(Tm) Advanced Biofuels Llc Increased heterologous Fe-S enzyme actiivty in yeast
AU2009296227A1 (en) 2008-09-29 2010-04-01 Butamax(Tm) Advanced Biofuels Llc Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
AU2009320163B2 (en) 2008-10-27 2014-10-02 Butamax(Tm) Advanced Biofuels Llc Carbon pathway optimized production hosts for the production of isobutanol
WO2010051527A2 (en) 2008-10-31 2010-05-06 Gevo, Inc. Engineered microorganisms capable of producing target compounds under anaerobic conditions
US8465964B2 (en) 2008-11-13 2013-06-18 Butamax (TM) Advanced Biofules LLC Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
US8828694B2 (en) 2008-11-13 2014-09-09 Butamax Advanced Biofuels Llc Production of isobutanol in yeast mitochondria
US8652823B2 (en) 2008-12-03 2014-02-18 Butamax(Tm) Advanced Biofuels Llc Strain for butanol production with increased membrane unsaturated trans fatty acids
US8795992B2 (en) 2008-12-29 2014-08-05 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall integrity pathway
US8455225B2 (en) 2008-12-29 2013-06-04 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
US8557562B2 (en) 2008-12-29 2013-10-15 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving filamentous growth response
US8614085B2 (en) 2009-02-27 2013-12-24 Butamax(Tm) Advanced Biofuels Llc Yeast with increased butanol tolerance involving a multidrug efflux pump gene
US20120058541A1 (en) 2009-05-22 2012-03-08 Butamax(Tm) Advanced Biofuels Llc Engineering resistance to aliphatic alcohols
US8232089B2 (en) 2009-08-12 2012-07-31 Gevo, Inc. Cytosolic isobutanol pathway localization for the production of isobutanol
BR112012006939A2 (pt) 2009-09-29 2015-09-08 Butamax Tm Advanced Biofuels célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides
AU2010300722A1 (en) 2009-09-29 2012-04-12 Butamax(Tm) Advanced Biofuels Llc Improved flux to acetolactate-derived products in lactic acid bacteria
JP5805094B2 (ja) 2009-09-29 2015-11-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 高有効性のケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生産
US20110195505A1 (en) 2009-10-08 2011-08-11 Butamax(Tm) Advanced Biofuels Llc Bacterial strains for butanol production
GB2487889B (en) 2009-11-24 2012-12-19 Gevo Inc Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
AU2010339511A1 (en) 2009-12-29 2012-07-05 Butamax(Tm) Advanced Biofuels Llc Expression of hexose kinase in recombinant host cells
EP2519628A2 (en) 2009-12-29 2012-11-07 Butamax (TM) Advanced Biofuels LLC Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
EP2534240B1 (en) 2010-02-12 2019-06-05 GEVO, Inc. Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids
KR20130027063A (ko) 2010-02-17 2013-03-14 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 Ｆｅ-ｓ 클러스터 요구성 단백질의 활성 향상
MX2012014550A (es) * 2010-06-17 2013-01-29 Butamax Tm Advanced Biofuels Cultivo de produccion de levadura para la produccion de butanol.
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
CA2810244C (en) 2010-09-07 2020-10-06 Butamax(Tm) Advanced Biofuels Llc Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion
US9790521B2 (en) 2011-03-24 2017-10-17 Butamax Advanced Biofuels Llc Host cells and methods for production of isobutanol
US20120258873A1 (en) 2011-04-06 2012-10-11 Butamax(Tm) Advanced Biofuels Llc Reduction of 2,3-dihydroxy-2-methyl butyrate (dhmb) in butanol production
CN103814135A (zh) 2011-06-17 2014-05-21 布特马斯先进生物燃料有限责任公司 木质纤维素水解产物作为原料用于异丁醇发酵
CN105473726B (zh) 2011-07-28 2021-03-12 布特马斯先进生物燃料有限责任公司 酮异戊酸脱羧酶及其使用方法
JP2015503350A (ja) 2011-12-30 2015-02-02 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー アルコールの発酵生産
WO2013102147A2 (en) 2011-12-30 2013-07-04 Butamax (Tm) Advanced Biofuels Llc Genetic switches for butanol production
AU2013235391A1 (en) 2012-03-23 2014-09-04 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
CA2873109A1 (en) 2012-05-11 2013-11-28 Butamax Advanced Biofuels Llc Ketol-acid reductoisomerase enzymes and methods of use
BR112015006691A2 (pt) 2012-09-26 2017-12-12 Butamax Advanced Biofuels Llc métodos, célula hospedeira recombinante, composição, polipeptídeo, polinucleotídeos e vetor recombinante
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
WO2014106107A2 (en) 2012-12-28 2014-07-03 Butamax (Tm) Advanced Biofuels Llc Dhad variants for butanol production
US20140186911A1 (en) 2012-12-28 2014-07-03 Butamax Advanced Biofuels Llc Recombinant host cells and methods for producing butanol
CN105378057A (zh) 2013-03-14 2016-03-02 纳幕尔杜邦公司 用于丁醇生产的甘油3-磷酸脱氢酶
WO2014151190A1 (en) 2013-03-15 2014-09-25 Butamax Advanced Biofuels Llc Dhad variants and methods of screening

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