JP2015514128A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015514128A5 JP2015514128A5 JP2015505016A JP2015505016A JP2015514128A5 JP 2015514128 A5 JP2015514128 A5 JP 2015514128A5 JP 2015505016 A JP2015505016 A JP 2015505016A JP 2015505016 A JP2015505016 A JP 2015505016A JP 2015514128 A5 JP2015514128 A5 JP 2015514128A5
- Authority
- JP
- Japan
- Prior art keywords
- monomer
- lysenin
- pore
- variant
- substitution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102200028065 CSN1S1 E92Q Human genes 0.000 claims 40
- 102220389474 PABPC4L E84Q Human genes 0.000 claims 39
- 102200010697 JPH2 E85K Human genes 0.000 claims 38
- 102200029302 CLHC1 E167A Human genes 0.000 claims 24
- 239000000178 monomer Substances 0.000 claims 22
- 101700012748 TXL Proteins 0.000 claims 13
- 239000011148 porous material Substances 0.000 claims 8
- 238000006467 substitution reaction Methods 0.000 claims 7
- 235000001014 amino acid Nutrition 0.000 claims 6
- 150000001413 amino acids Chemical group 0.000 claims 6
- 239000012491 analyte Substances 0.000 claims 6
- 229920000023 polynucleotide Polymers 0.000 claims 6
- 239000002157 polynucleotide Substances 0.000 claims 6
- 102220395611 CHEK2 E76S Human genes 0.000 claims 2
- 102200033873 PTGDR2 E71S Human genes 0.000 claims 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 238000005259 measurement Methods 0.000 claims 2
- 102200059443 ANAPC11 H58S Human genes 0.000 claims 1
- 239000004475 Arginine Substances 0.000 claims 1
- 229960001230 Asparagine Drugs 0.000 claims 1
- 102200040453 BEX1 N48S Human genes 0.000 claims 1
- MTCFGRXMJLQNBG-UWTATZPHSA-N D-serine Chemical compound OC[C@@H](N)C(O)=O MTCFGRXMJLQNBG-UWTATZPHSA-N 0.000 claims 1
- 102200070587 DGUOK N46S Human genes 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 1
- 239000004472 Lysine Substances 0.000 claims 1
- 102200029438 MIER3 D126G Human genes 0.000 claims 1
- 102200153983 OR4L1 R52S Human genes 0.000 claims 1
- 102220389295 PABPC4L E84S Human genes 0.000 claims 1
- 102200086922 SMN2 F70S Human genes 0.000 claims 1
- 102200132707 SOD1 H81S Human genes 0.000 claims 1
- 235000004279 alanine Nutrition 0.000 claims 1
- -1 aromatic amino acids Chemical class 0.000 claims 1
- 235000009582 asparagine Nutrition 0.000 claims 1
- 102000024070 binding proteins Human genes 0.000 claims 1
- 108091007650 binding proteins Proteins 0.000 claims 1
- 125000003636 chemical group Chemical group 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 102220198313 rs1057519973 Human genes 0.000 claims 1
- 230000003335 steric effect Effects 0.000 claims 1
Claims (15)
- 配列番号２に示す配列の変種を含む変異体ライセニン（lysenin）モノマーであって、前記モノマーがポアを形成でき、前記変種が、配列番号２のアミノ酸配列と配列全長にわたって少なくとも８５％の相同性を有し、前記変種が、ポリヌクレオチドと相互作用するモノマーの能力を変更する配列番号２のＥ８４及びＥ９２の位置での置換を含む、変異体ライセニンモノマー。
- 前記変種が、ポリヌクレオチドと相互作用するモノマーの能力を変更する配列番号２のＥ８５、Ｅ９７及びＤ１２６の位置での置換を含む、請求項１に記載の変異体ライセニンモノマー。
- 前記置換が、（ａ）前記モノマーの立体効果を変更する、（ｂ）前記モノマーの正味電荷を変更する、（ｃ）前記ポリヌクレオチドと水素結合する前記モノマーの能力を変更する、（ｄ）非局在化電子ｐｉシステムを通じて相互作用する化学的基を導入するもしくは除去する、および／または（ｅ）モノマーの構造を変更する、請求項１に記載の変異体ライセニンモノマー。
- （ｉ）前記置換が、正味の正電荷を増加または減少させ、（ｉｉ）前記置換が、Ｅ８４又はＥ９２に正に荷電したアミノ酸を導入することによりまたはＥ８４及びＥ９２で負電荷を中和することによって前記正味の正電荷を増加させ、または（ｉｉｉ）前記置換が、前記負に荷電したアミノ酸を１つもしくは複数の無荷電アミノ酸、非極性アミノ酸および／もしくは芳香族アミノ酸で置換することによって、Ｅ８４及びＥ９２で負電荷を中和する、請求項３に記載の変異体ライセニンモノマー。
- Ｅ８４およびＥ９２の位置で置換されたアミノ酸が、アスパラギン（Ｎ）、セリン（Ｓ）、グルタミン（Ｑ）、アルギニン（Ｒ）、グリシン（Ｇ）、チロシン（Ｙ）、ロイシン（Ｌ）、リジン（Ｋ）またはアラニン（Ａ）から選択される、請求項１に記載の変異体ライセニンモノマー。
- 前記変種が次の置換：
ｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６ＧおよびＥ１６７Ａの１つもしくは複数；
ｉｉ．Ｅ７６Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６ＧおよびＥ１６７Ａの１つもしくは複数；
ｉｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ５０Ｓの１つもしくは複数；
ｉｖ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ７１Ｓの１つもしくは複数；
ｖ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ９４Ｓの１つもしくは複数；
ｖｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ１０２Ｓの１つもしくは複数；
ｖｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ１２８Ｓの１つもしくは複数；
ｖｉｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ１３５Ｓの１つもしくは複数；
ｉｘ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＤ６８Ｓの１つもしくは複数；
ｘ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＤ１２１Ｓの１つもしくは複数；
ｘｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＤ１３４Ｓの１つもしくは複数；または
ｘｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６ＧおよびＥ１３５Ｓの１つもしくは複数；
ｘｉｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｈ１０３ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｉｖ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｍ９０Ｓ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｖ．Ｅ８４Ｑ、Ｑ８７Ｓ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｖｉ．Ｅ８４Ｑ、Ｅ８５Ｓ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｖｉｉ．Ｅ８４Ｓ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｖｉｉｉ．Ｈ８１Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｉｘ．Ｙ７９Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘ．Ｆ７０Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｉ．Ｈ５８Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｉｉ．Ｒ５２Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｉｉｉ．Ｎ４８Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｉｖ．Ｎ４６Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｖ．Ｍ４４Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｖｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２ＱおよびＥ９７Ｓの１つもしくは複数；
ｘｘｖｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＤ１２６Ｇの１つもしくは複数；
ｘｘｖｉｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７ＳおよびＥ１６７Ａの１つもしくは複数；
ｘｘｉｘ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｄ１２６ＧおよびＥ１６７Ａの１つもしくは複数；
ｘｘｘ．Ｅ８４Ｑ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６ＧおよびＥ１６７Ａの１つもしくは複数；
ｘｘｘｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＤ１２１Ｓの１つもしくは複数；
ｘｘｘｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＤ６８Ｓの１つもしくは複数；
ｘｘｘｉｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ１３５Ｓの１つもしくは複数；
ｘｘｘｉｖ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ１２８Ｓの１つもしくは複数；
ｘｘｘｖ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ１０２Ｓの１つもしくは複数；
ｘｘｘｖｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ９４Ｓの１つもしくは複数；
ｘｘｘｖｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ７１Ｓの１つもしくは複数；
ｘｘｘｖｉｉｉ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６Ｇ、Ｅ１６７ＡおよびＥ５０Ｓの１つもしくは複数；
ｘｘｘｉｘ．Ｅ７６Ｓ、Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６ＧおよびＥ１６７Ａの１つもしくは複数；または
ｘｌ．Ｅ８４Ｑ、Ｅ８５Ｋ、Ｅ９２Ｑ、Ｅ９７Ｓ、Ｄ１２６ＧおよびＥ１６７Ａの１つもしくは複数
を含む、請求項１に記載の変異体ライセニンモノマー。 - 前記変種がｉからｘｌのいずれか１つの全ての置換を含む、請求項６に記載の変異体ライセニンモノマー。
- ライセニン由来の２つ以上の共有結合的に付着されたモノマーを含む構築物であって、モノマーの少なくとも１つが請求項１から７のいずれか一項に記載の変異体ライセニンモノマーである、構築物。
- （ｉ）前記２つ以上のモノマーが同じであるまたは異なっている、および／または
（ｉｉ）少なくとも１つのモノマーが配列番号２に示す配列を含み、および／または
（ｉｉｉ）前記構築物が２つのモノマーを含み、および／または
（ｉｖ）前記モノマーが遺伝子的に融合されており、および／または
（ｖ）前記モノマーがリンカーを介して付着している、
請求項８に記載の構築物。 - 請求項１から７のいずれか一項に記載の変異体ライセニンモノマーまたは請求項８に記載の構築物をコードしているポリヌクレオチド。
- 請求項１から７のいずれか一項に記載の十分な数の変異体ライセニンモノマーを含むライセニン由来のホモオリゴマーポア。
- 請求項１から７のいずれか一項に記載の少なくとも１つの変異体ライセニンモノマーを含むライセニン由来のヘテロオリゴマーポア。
- （ａ）標的分析物を請求項１１または１２に記載のポアに接触させて、前記標的分析物が前記ポアを通って移動するようにするステップ、および
（ｂ）前記分析物が前記ポアに対して移動するときに１つまたは複数の測定を行うステップであって、前記測定が標的分析物の１つまたは複数の特性を示し、それにより標的分析物を特性決定するステップ
を含む、標的分析物を特性決定する方法。 - 標的分析物を特性決定するための請求項１１または１２に記載のポアの使用。
- （ａ）請求項１１または１２に記載のポアおよび（ｂ）ポリヌクレオチド結合タンパク質を含む、標的ポリヌクレオチドを特性決定するためのキット。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261622174P | 2012-04-10 | 2012-04-10 | |
| US61/622,174 | 2012-04-10 | ||
| PCT/GB2013/050667 WO2013153359A1 (en) | 2012-04-10 | 2013-03-15 | Mutant lysenin pores |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017250647A Division JP2018075025A (ja) | 2012-04-10 | 2017-12-27 | 変異体ライセニンポア |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015514128A JP2015514128A (ja) | 2015-05-18 |
| JP2015514128A5 true JP2015514128A5 (ja) | 2016-04-28 |
| JP6271505B2 JP6271505B2 (ja) | 2018-01-31 |
Family
ID=47915292
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015505016A Active JP6271505B2 (ja) | 2012-04-10 | 2013-03-15 | 変異体ライセニンポア |
| JP2017250647A Pending JP2018075025A (ja) | 2012-04-10 | 2017-12-27 | 変異体ライセニンポア |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017250647A Pending JP2018075025A (ja) | 2012-04-10 | 2017-12-27 | 変異体ライセニンポア |
Country Status (9)
| Country | Link |
|---|---|
| US (4) | US9777049B2 (ja) |
| EP (2) | EP2836506B1 (ja) |
| JP (2) | JP6271505B2 (ja) |
| KR (1) | KR102083695B1 (ja) |
| CN (2) | CN104350067A (ja) |
| AU (1) | AU2013246702B2 (ja) |
| BR (1) | BR112014025157B1 (ja) |
| CA (1) | CA2869546C (ja) |
| WO (1) | WO2013153359A1 (ja) |
Families Citing this family (130)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2008217579A1 (en) | 2007-02-20 | 2008-08-28 | Oxford Nanopore Technologies Limited | Formation of lipid bilayers |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| CA2750879C (en) | 2009-01-30 | 2018-05-22 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| BR112013020411B1 (pt) | 2011-02-11 | 2021-09-08 | Oxford Nanopore Technologies Limited | Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo |
| EP3633370B1 (en) | 2011-05-27 | 2024-05-01 | Oxford Nanopore Technologies plc | Method and apparatus for determining the presence, absence or characteristics of an analyte |
| BR112014001699A2 (pt) | 2011-07-25 | 2017-06-13 | Oxford Nanopore Tech Ltd | método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho |
| CA2852812A1 (en) | 2011-10-21 | 2013-04-25 | Oxford Nanopore Technologies Limited | Enzyme method |
| EP2798084B1 (en) | 2011-12-29 | 2017-04-19 | Oxford Nanopore Technologies Limited | Enzyme method |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| CN104220874B (zh) | 2012-02-15 | 2017-05-24 | 牛津纳米孔技术公司 | 适配体方法 |
| KR102106499B1 (ko) | 2012-02-16 | 2020-05-04 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 폴리머의 측정의 분석 |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| EP2875128B8 (en) | 2012-07-19 | 2020-06-24 | Oxford Nanopore Technologies Limited | Modified helicases |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| EP2875152B1 (en) | 2012-07-19 | 2019-10-09 | Oxford Nanopore Technologies Limited | Enzyme construct |
| US9551023B2 (en) | 2012-09-14 | 2017-01-24 | Oxford Nanopore Technologies Ltd. | Sample preparation method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CN118086476A (zh) | 2013-10-18 | 2024-05-28 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| ES2735015T3 (es) | 2013-11-26 | 2019-12-13 | Illumina Inc | Composiciones y métodos para secuenciar polinucleótidos |
| CA2937411C (en) | 2014-01-22 | 2023-09-26 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| US10359395B2 (en) | 2014-02-19 | 2019-07-23 | University Of Washington | Nanopore-based analysis of protein characteristics |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| SG11201609692SA (en) | 2014-06-03 | 2016-12-29 | Illumina Inc | Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores |
| US10982290B2 (en) | 2014-07-25 | 2021-04-20 | Global Life Sciences Solutions Operations UK Ltd | Screening and monitoring the progression of type 2 diabetes by the molecular identification of human gut flora using FTA as a faecal collection device |
| CN117164684A (zh) | 2014-09-01 | 2023-12-05 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| WO2016055778A1 (en) | 2014-10-07 | 2016-04-14 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| CN115851894A (zh) | 2014-10-16 | 2023-03-28 | 牛津楠路珀尔科技股份有限公司 | 聚合物的分析 |
| CN113981055A (zh) | 2014-10-17 | 2022-01-28 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| JP6800862B2 (ja) | 2015-02-05 | 2020-12-16 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体通路を含むナノポアセンサ |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| GB201508003D0 (en) | 2015-05-11 | 2015-06-24 | Oxford Nanopore Tech Ltd | Apparatus and methods for measuring an electrical current |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| RU2713396C2 (ru) | 2015-06-03 | 2020-02-05 | Иллюмина, Инк. | Композиции, системы и способы секвенирования полинуклеотидов с применением соединительных структур, прикрепленных к полимеразам, расположенным вблизи нанопор |
| KR20180089499A (ko) | 2015-12-08 | 2018-08-08 | 카트호리이케 유니버시타이트 로이펜 | 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도 |
| JP6579948B2 (ja) * | 2015-12-24 | 2019-09-25 | 株式会社日立ハイテクノロジーズ | 生体ポリマを分析するための測定試薬及び分析デバイス |
| CN116356000A (zh) | 2016-03-02 | 2023-06-30 | 牛津纳米孔科技公开有限公司 | 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔 |
| EP4397970A3 (en) * | 2016-04-06 | 2024-10-09 | Oxford Nanopore Technologies PLC | Mutant pore |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| US20190203288A1 (en) | 2016-05-25 | 2019-07-04 | Oxford Nanopore Technologies Ltd. | Method |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201617886D0 (en) | 2016-10-21 | 2016-12-07 | Oxford Nanopore Technologies Limited | Method |
| GB201619930D0 (en) | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| US11840556B2 (en) | 2017-02-10 | 2023-12-12 | Oxford Nanopore Technologies Plc | Modified nanopores, compositions comprising the same, and uses thereof |
| WO2018152050A1 (en) | 2017-02-14 | 2018-08-23 | Axbio Inc. | Apparatus and methods for continuous diagnostics of macromolecules |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| GB201707138D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Machine learning analysis of nanopore measurements |
| EP3645552B1 (en) | 2017-06-30 | 2023-06-28 | Vib Vzw | Novel protein pores |
| GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB201808556D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| CN112203767B (zh) | 2018-05-24 | 2023-04-11 | 牛津纳米孔科技公司 | 电润湿装置中的液滴界面 |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB201814369D0 (en) | 2018-09-04 | 2018-10-17 | Oxford Nanopore Tech Ltd | Method for determining a polymersequence |
| CA3118808A1 (en) | 2018-11-08 | 2020-05-14 | Oxford Nanopore Technologies Limited | Pore |
| GB201818216D0 (en) | 2018-11-08 | 2018-12-26 | Oxford Nanopore Tech Ltd | Pore |
| GB201819378D0 (en) | 2018-11-28 | 2019-01-09 | Oxford Nanopore Tech Ltd | Analysis of nanopore signal using a machine-learning technique |
| WO2020109800A1 (en) | 2018-11-28 | 2020-06-04 | Oxford Nanopore Technologies Ltd. | Sensing system and method of operation |
| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| WO2020183172A1 (en) | 2019-03-12 | 2020-09-17 | Oxford Nanopore Technologies Inc. | Nanopore sensing device and methods of operation and of forming it |
| GB2580988B (en) | 2019-03-19 | 2022-04-13 | Oxford Nanopore Tech Ltd | Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity |
| CN110093399A (zh) * | 2019-04-22 | 2019-08-06 | 武汉大学 | 一种利用二磷酸尿嘧啶脱氧核苷酸检测核酸中腺嘌呤n6位发生甲基化修饰的方法 |
| GB201907243D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201915480D0 (en) | 2019-10-25 | 2019-12-11 | Oxford Nanopore Tech Ltd | Improved nanopore sensing device, components and method of manufacture |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| EP4270008A3 (en) | 2019-12-02 | 2024-01-10 | Oxford Nanopore Technologies PLC | Method of characterising a target polypeptide using a nanopore |
| GB201917742D0 (en) | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| GB202016874D0 (en) | 2020-10-23 | 2020-12-09 | Oxford Nanopore Tech Ltd | Nanopore support structure and manufacture thereof |
| AU2021291140A1 (en) | 2020-06-18 | 2023-02-02 | Oxford Nanopore Technologies Limited | Method of characterising a polynucleotide moving through a nanopore |
| WO2021255475A1 (en) | 2020-06-18 | 2021-12-23 | Oxford Nanopore Technologies Limited | A method of selectively characterising a polynucleotide using a detector |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| CN115989410A (zh) | 2020-07-17 | 2023-04-18 | 牛津纳米孔科技公开有限公司 | 纳米孔感测装置 |
| US20230266268A1 (en) | 2020-07-22 | 2023-08-24 | Oxford Nanopore Technologies Inc. | Solid state nanopore formation |
| US20230377649A1 (en) | 2020-09-29 | 2023-11-23 | Ecole Polytechnique Federale De Lausanne (Epfl) | Systems And Methods For Digital Information Decoding And Data Storage In Hybrid Macromolecules |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| CN117255945A (zh) | 2021-03-31 | 2023-12-19 | 伊鲁米纳公司 | 纳米孔传感器装置 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| WO2022243692A1 (en) | 2021-05-19 | 2022-11-24 | Oxford Nanopore Technologies Plc | Methods for complement strand sequencing |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| GB2610380A (en) | 2021-08-23 | 2023-03-08 | Cambridge Entpr Ltd | Nucleic acid detection |
| GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
| KR20240067097A (ko) | 2021-09-22 | 2024-05-16 | 일루미나, 인코포레이티드 | 나노공극을 사용한 폴리뉴클레오타이드 시퀀싱 |
| GB202113935D0 (en) | 2021-09-29 | 2021-11-10 | Cambridge Entpr Ltd | Nucleic acid characterisation |
| WO2023081031A1 (en) | 2021-11-08 | 2023-05-11 | Illumina, Inc. | Identifying nucleotides using changes in impedance between electrodes |
| WO2023094806A1 (en) | 2021-11-29 | 2023-06-01 | Oxford Nanopore Technologies Plc | Nanopore measurement signal analysis |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| WO2023187110A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Amphiphilic polymers to be used in barriers and preparation thereof, barriers with nanopores and preparation thereof |
| US20240076322A1 (en) | 2022-03-31 | 2024-03-07 | Illumina Cambridge Limited | Barriers including cross-linked amphiphilic molecules, and methods of making the same |
| AU2023246642A1 (en) | 2022-03-31 | 2024-08-29 | Illumina, Inc. | Nanopore devices including barriers using diblock or triblock copolymers, and methods of making the same |
| US20230391961A1 (en) | 2022-03-31 | 2023-12-07 | Illumina Cambridge Limited | Methods for inserting nanopores into polymeric membranes using chaotropic solvents |
| WO2023187001A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Devices including osmotically balanced barriers, and methods of making and using the same |
| WO2023187112A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Amphiphilic block-co-polymers cross-linked with different cross-linkers consecutively forming a cross-linking hierarchy |
| AU2023244507A1 (en) | 2022-03-31 | 2024-08-29 | Illumina, Inc. | Barriers including biological nanopore for dna sequencing, the barriers being made of co-polymers with end and/or middle groups, and methods of making the same |
| WO2023222657A1 (en) | 2022-05-17 | 2023-11-23 | Oxford Nanopore Technologies Plc | Method and adaptors |
| GB202207267D0 (en) | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
| WO2023229884A2 (en) | 2022-05-27 | 2023-11-30 | Illumina, Inc. | 3'-blocked nucleotides, methods of deblocking the same, and methods of synthesizing polynucleotides using the same |
| GB202215442D0 (en) | 2022-10-19 | 2022-11-30 | Oxford Nanopore Tech Plc | Analysis of a polymer |
| EP4362028A1 (en) | 2022-10-31 | 2024-05-01 | Ecole Polytechnique Federale De Lausanne (Epfl) | Mutant aerolysin and uses thereof |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| WO2024094966A1 (en) | 2022-11-01 | 2024-05-10 | Oxford Nanopore Technologies Plc | Biochemical analysis system and method of controlling a biochemical analysis system |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202301095D0 (en) | 2023-01-25 | 2023-03-08 | Oxford Nanopore Tech Plc | Calibration of a nanopore array device |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202401864D0 (en) | 2024-02-12 | 2024-03-27 | Fund Centre De Regulacio Genòmica | A method of detecting non-canonical bases in sequencing data |
| GB202407228D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
Family Cites Families (151)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE56026B1 (en) | 1982-10-19 | 1991-03-27 | Cetus Corp | Cysteine-depleted muteins of biologically active proteins |
| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| WO1994025616A1 (en) | 1993-04-28 | 1994-11-10 | Worcester Foundation For Experimental Biology | Cell-targeted lytic pore-forming agents |
| US5777078A (en) | 1993-04-28 | 1998-07-07 | Worcester Foundation For Experimental Biology | Triggered pore-forming agents |
| DE4320201A1 (de) | 1993-06-18 | 1995-01-12 | Asta Medica Ag | Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation |
| US5386373A (en) | 1993-08-05 | 1995-01-31 | Pavilion Technologies, Inc. | Virtual continuous emission monitoring system with sensor validation |
| US5561043A (en) | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
| US7569341B2 (en) | 1994-01-31 | 2009-08-04 | Trustees Of Boston University | Nucleic acid directed immobilization arrays and methods of assembly |
| US6362002B1 (en) * | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| JP3891620B2 (ja) | 1996-11-14 | 2007-03-14 | 株式会社アイシン・コスモス研究所 | ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法 |
| US20020197614A1 (en) | 1997-05-16 | 2002-12-26 | Mosaic Technologies, Inc. | Electrophoretic analysis of target molecules using adapter molecules |
| AU8586298A (en) | 1997-07-25 | 1999-02-16 | University Of Massachusetts | Designed protein pores as components for biosensors |
| US6087099A (en) | 1997-09-08 | 2000-07-11 | Myriad Genetics, Inc. | Method for sequencing both strands of a double stranded DNA in a single sequencing reaction |
| US6127166A (en) | 1997-11-03 | 2000-10-03 | Bayley; Hagan | Molluscan ligament polypeptides and genes encoding them |
| JPH11137260A (ja) | 1997-11-06 | 1999-05-25 | Soyaku Gijutsu Kenkyusho:Kk | 抗インフルエンザウイルス環状ダンベル型ｒｎａ−ｄｎａキメラ化合物及び抗インフルエンザウイルス剤 |
| US6123819A (en) | 1997-11-12 | 2000-09-26 | Protiveris, Inc. | Nanoelectrode arrays |
| DE19826758C1 (de) | 1998-06-15 | 1999-10-21 | Soft Gene Gmbh | Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte |
| US6743605B1 (en) | 1998-06-24 | 2004-06-01 | Enzo Life Sciences, Inc. | Linear amplification of specific nucleic acid sequences |
| US7155344B1 (en) | 1998-07-27 | 2006-12-26 | Caliper Life Sciences, Inc. | Distributed database for analytical instruments |
| US6150112A (en) | 1998-09-18 | 2000-11-21 | Yale University | Methods for identifying DNA sequences for use in comparison of DNA samples by their lack of polymorphism using Y shape adaptors |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
| NO986133D0 (no) | 1998-12-23 | 1998-12-23 | Preben Lexow | FremgangsmÕte for DNA-sekvensering |
| EP1192453B1 (en) | 1999-06-22 | 2012-02-15 | President and Fellows of Harvard College | Molecular and atomic scale evaluation of biopolymers |
| EP1196434A2 (en) | 1999-06-29 | 2002-04-17 | University Health Network | Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes |
| CA2381139A1 (en) | 1999-08-31 | 2001-03-08 | Michael Niederweis | Method for the production of a channel forming protein |
| WO2001040516A2 (en) | 1999-12-02 | 2001-06-07 | Molecular Staging Inc. | Generation of single-strand circular dna from linear self-annealing segments |
| EP2261240B1 (en) | 2000-02-11 | 2015-09-02 | The Texas A & M University System | Biosensor compositions and methods of use |
| AU2001243232A1 (en) | 2000-02-25 | 2001-09-03 | University Of South Carolina Research Foundation | Topoisomerase linker-mediated amplification methods |
| EP1290005A4 (en) | 2000-03-21 | 2005-04-20 | Curagen Corp | VEGF MODULATED GENES AND METHODS OF USE |
| ATE382055T1 (de) | 2000-03-22 | 2008-01-15 | Curagen Corp | Wnt-1 verwandte polypeptide und dafür kodierende nukleinsäuren |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| DE10146902A1 (de) | 2000-09-25 | 2002-09-19 | Sensovation Ag | Bildsensor, Vorrichtung und Verfahren für optische Messungen |
| WO2002042496A2 (en) | 2000-11-27 | 2002-05-30 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US20030087232A1 (en) | 2001-01-25 | 2003-05-08 | Fred Christians | Methods for screening polypeptides |
| US7807408B2 (en) | 2001-03-19 | 2010-10-05 | President & Fellows Of Harvard College | Directed evolution of proteins |
| US6995236B2 (en) * | 2001-05-08 | 2006-02-07 | Riken | Sphingomyelin detecting probe |
| JP2002355035A (ja) * | 2001-05-08 | 2002-12-10 | Inst Of Physical & Chemical Res | スフィンゴミエリン検出プローブ |
| US6863833B1 (en) | 2001-06-29 | 2005-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Microfabricated apertures for supporting bilayer lipid membranes |
| WO2003004992A2 (en) | 2001-07-03 | 2003-01-16 | The Regents Of The University Of California | Mammalian sweet and amino acid heterodimeric taste receptors |
| JP3983738B2 (ja) | 2001-08-31 | 2007-09-26 | ジェンテクス・コーポレーション | ヒートシンクを有する車のランプ組立体 |
| IL163822A0 (en) | 2002-03-15 | 2005-12-18 | Nuevolution As | An improved method for synthesising templated molecules |
| AU2003245272A1 (en) | 2002-05-10 | 2003-11-11 | The Texas A And M University System | Stochastic sensing through covalent interactions |
| WO2004081183A2 (en) | 2003-03-07 | 2004-09-23 | Rubicon Genomics, Inc. | In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna |
| CA2519309A1 (en) | 2003-03-25 | 2004-10-14 | Stratagene California | Dna polymerase fusions and uses thereof |
| US7163658B2 (en) | 2003-04-23 | 2007-01-16 | Rouvain Bension | Rapid sequencing of polymers |
| US7344882B2 (en) | 2003-05-12 | 2008-03-18 | Bristol-Myers Squibb Company | Polynucleotides encoding variants of the TRP channel family member, LTRPC3 |
| AU2003904237A0 (en) | 2003-08-08 | 2003-08-21 | Garvan Institute Of Medical Research | Novel translocation assay |
| WO2005056750A2 (en) | 2003-12-11 | 2005-06-23 | Quark Biotech, Inc. | Inversion-duplication of nucleic acids and libraries prepared thereby |
| EP1721283B1 (en) | 2004-02-06 | 2022-11-30 | Council of Scientific and Industrial Research | Computational method for identifying adhesin and adhesin-like proteins of therapeutic potential |
| JP2005253427A (ja) | 2004-03-15 | 2005-09-22 | Aisin Seiki Co Ltd | 核酸検出方法及び核酸単離方法 |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| EP1784754A4 (en) | 2004-08-13 | 2009-05-27 | Harvard College | OPTI-NANOPORE DNA READING PLATFORM WITH ULTRAHOLE THROUGHPUT |
| US20060105461A1 (en) | 2004-10-22 | 2006-05-18 | May Tom-Moy | Nanopore analysis system |
| WO2007084103A2 (en) | 2004-12-21 | 2007-07-26 | The Texas A & M University System | High temperature ion channels and pores |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| US7272518B2 (en) | 2005-07-01 | 2007-09-18 | Square D Company | Automated hierarchy classification in utility monitoring systems |
| WO2007024997A2 (en) | 2005-08-22 | 2007-03-01 | Fermalogic, Inc. | Methods of increasing production of secondary metabolites |
| US7877154B2 (en) | 2005-09-30 | 2011-01-25 | Fisher-Rosemount Systems, Inc. | Method and system for controlling a batch process |
| KR100730350B1 (ko) | 2005-10-17 | 2007-06-19 | 삼성전자주식회사 | 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치 |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| CA2633476C (en) | 2005-12-22 | 2015-04-21 | Pacific Biosciences Of California, Inc. | Active surface coupled polymerases |
| US7932029B1 (en) | 2006-01-04 | 2011-04-26 | Si Lok | Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities |
| US7849581B2 (en) | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| US20110039776A1 (en) | 2006-09-06 | 2011-02-17 | Ashutosh Chilkoti | Fusion peptide therapeutic compositions |
| US7638034B2 (en) | 2006-09-21 | 2009-12-29 | Los Alamos National Security, Llc | Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions |
| WO2008045575A2 (en) | 2006-10-13 | 2008-04-17 | J. Craig Venter Institute, Inc. | Sequencing method |
| US8594848B2 (en) | 2006-11-28 | 2013-11-26 | Lester F. Ludwig | Reconfigurable chemical process systems |
| AU2008217579A1 (en) | 2007-02-20 | 2008-08-28 | Oxford Nanopore Technologies Limited | Formation of lipid bilayers |
| EP2156179B1 (en) | 2007-04-04 | 2021-08-18 | The Regents of The University of California | Methods for using a nanopore |
| US8158344B2 (en) | 2007-06-29 | 2012-04-17 | Duke University | Methods and compositions for correlating genetic markers with multiple sclerosis |
| GB0716005D0 (en) | 2007-08-16 | 2007-09-26 | Imp Innovations Ltd | Single molecule spectroscopy using nanoporpus membranes |
| GB0716264D0 (en) | 2007-08-21 | 2007-09-26 | Isis Innovation | Bilayers |
| EP2195648B1 (en) | 2007-09-12 | 2019-05-08 | President and Fellows of Harvard College | High-resolution molecular graphene sensor comprising an aperture in the graphene layer |
| GB2453377A (en) | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
| WO2009052214A2 (en) | 2007-10-15 | 2009-04-23 | Complete Genomics, Inc. | Sequence analysis using decorated nucleic acids |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| WO2009084721A1 (en) | 2007-12-31 | 2009-07-09 | Fujirebio Inc. | Clusters of microresonators for cavity mode optical sensing |
| US8231969B2 (en) | 2008-03-26 | 2012-07-31 | University Of Utah Research Foundation | Asymmetrically functionalized nanoparticles |
| AU2009229157B2 (en) | 2008-03-28 | 2015-01-29 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
| EP2293921A4 (en) | 2008-05-22 | 2013-05-22 | Univ California | MEMBRANE PROBLEMS AND MEMBRANES MADE FROM THESE |
| US8652771B2 (en) | 2008-05-28 | 2014-02-18 | University of Souther California | Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects |
| WO2010004273A1 (en) | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| CN103695530B (zh) | 2008-07-07 | 2016-05-25 | 牛津纳米孔技术有限公司 | 酶-孔构建体 |
| HUE029215T2 (en) | 2008-09-22 | 2017-02-28 | Univ Washington | MSP nanopores and related procedures |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| CA2744821A1 (en) | 2008-11-26 | 2010-06-03 | Illumina, Inc. | Methods and systems for analysis of sequencing data |
| CA2750879C (en) | 2009-01-30 | 2018-05-22 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| AU2010209508C1 (en) | 2009-01-30 | 2017-10-19 | Oxford Nanopore Technologies Limited | Hybridization linkers |
| JP5861223B2 (ja) | 2009-02-23 | 2016-02-16 | サイトムエックス セラピューティクス, インク.CytomX Therapeutics, Inc. | プロタンパク質およびその使用方法 |
| FR2943656A1 (fr) | 2009-03-25 | 2010-10-01 | Air Liquide | Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| US8828208B2 (en) | 2009-04-20 | 2014-09-09 | Oxford Nanopore Technologies Limited | Lipid bilayer sensor array |
| CN102741430B (zh) | 2009-12-01 | 2016-07-13 | 牛津楠路珀尔科技有限公司 | 生化分析仪器、用于进行生化分析的第一模块以及相关方法 |
| FR2955773B1 (fr) | 2010-02-01 | 2017-05-26 | Commissariat A L'energie Atomique | Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination |
| WO2011106456A2 (en) | 2010-02-23 | 2011-09-01 | University Of Washington | Artificial mycolic acid membranes |
| WO2011125015A2 (en) | 2010-04-05 | 2011-10-13 | Bar-Ilan University | Protease-activatable pore-forming polypeptides |
| US9593370B2 (en) | 2010-10-01 | 2017-03-14 | Oxford Nanopore Technologies Ltd. | Biochemical analysis apparatus and rotary valve |
| CN102116783B (zh) | 2010-12-31 | 2013-05-29 | 北京普源精电科技有限公司 | 一种波形显示方法 |
| GB201100516D0 (en) | 2011-01-12 | 2011-02-23 | Isis Innovation | Method using fluorinated amphiphiles |
| CN102174554A (zh) | 2011-01-24 | 2011-09-07 | 内蒙古民族大学 | 双控双调节原核表达载体系统及其构建方法和用途 |
| BR112013020411B1 (pt) | 2011-02-11 | 2021-09-08 | Oxford Nanopore Technologies Limited | Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo |
| EP3633370B1 (en) | 2011-05-27 | 2024-05-01 | Oxford Nanopore Technologies plc | Method and apparatus for determining the presence, absence or characteristics of an analyte |
| WO2012166906A1 (en) | 2011-05-31 | 2012-12-06 | Massachusetts Institute Of Technology | Cell-directed synthesis of multifunctional nanopatterns and nanomaterials |
| BR112014001699A2 (pt) | 2011-07-25 | 2017-06-13 | Oxford Nanopore Tech Ltd | método para sequenciar de um polinucleotídeo alvo de filamento duplo, kit, métodos para preparar um polinucleotídeo alvo de filamento duplo para sequenciamento e para sequenciar um polinucleotídeo alvo de filamento duplo, e, aparelho |
| EP3269825B1 (en) | 2011-09-23 | 2020-02-19 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| CA2852812A1 (en) | 2011-10-21 | 2013-04-25 | Oxford Nanopore Technologies Limited | Enzyme method |
| WO2013098561A1 (en) | 2011-12-29 | 2013-07-04 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| EP2798084B1 (en) | 2011-12-29 | 2017-04-19 | Oxford Nanopore Technologies Limited | Enzyme method |
| US20130244340A1 (en) | 2012-01-20 | 2013-09-19 | Genia Technologies, Inc. | Nanopore Based Molecular Detection and Sequencing |
| CN104220874B (zh) | 2012-02-15 | 2017-05-24 | 牛津纳米孔技术公司 | 适配体方法 |
| WO2013153359A1 (en) | 2012-04-10 | 2013-10-17 | Oxford Nanopore Technologies Limited | Mutant lysenin pores |
| TWI655213B (zh) | 2012-07-13 | 2019-04-01 | 目立康股份有限公司 | 自我組織化肽衍生物的製造方法 |
| EP2875152B1 (en) | 2012-07-19 | 2019-10-09 | Oxford Nanopore Technologies Limited | Enzyme construct |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| EP2875128B8 (en) | 2012-07-19 | 2020-06-24 | Oxford Nanopore Technologies Limited | Modified helicases |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| WO2014064444A1 (en) | 2012-10-26 | 2014-05-01 | Oxford Nanopore Technologies Limited | Droplet interfaces |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| US20140206842A1 (en) | 2013-01-22 | 2014-07-24 | Muhammed Majeed | Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical |
| EP2954320B1 (en) | 2013-02-07 | 2018-04-18 | Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. | Hybrid nanopores and uses thereof for detection of analytes |
| CA2901545C (en) | 2013-03-08 | 2019-10-08 | Oxford Nanopore Technologies Limited | Use of spacer elements in a nucleic acid to control movement of a helicase |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| DK3553175T3 (da) | 2013-03-13 | 2021-08-23 | Illumina Inc | Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek |
| WO2014153047A1 (en) | 2013-03-14 | 2014-09-25 | The Trustees Of Boston University | Optoelectronic control of solid-state nanopores |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| WO2014187924A1 (en) | 2013-05-24 | 2014-11-27 | Illumina Cambridge Limited | Pyrophosphorolytic sequencing |
| WO2015051378A1 (en) | 2013-10-04 | 2015-04-09 | University Of Washington Through Its Center For Commercialization | Systems and methods for nanopore-based analysis of nucleic acids |
| CN118086476A (zh) | 2013-10-18 | 2024-05-28 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| ES2735015T3 (es) | 2013-11-26 | 2019-12-13 | Illumina Inc | Composiciones y métodos para secuenciar polinucleótidos |
| EP3087186A1 (en) | 2013-12-24 | 2016-11-02 | Vib Vzw | Secretion and functional display of chimeric polypeptides |
| CA2937411C (en) | 2014-01-22 | 2023-09-26 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10337060B2 (en) | 2014-04-04 | 2019-07-02 | Oxford Nanopore Technologies Ltd. | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| FR3023394B1 (fr) | 2014-07-02 | 2017-12-29 | Adn Access Data Networks | Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture |
| CN117164684A (zh) | 2014-09-01 | 2023-12-05 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| WO2016055778A1 (en) | 2014-10-07 | 2016-04-14 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| KR20180089499A (ko) | 2015-12-08 | 2018-08-08 | 카트호리이케 유니버시타이트 로이펜 | 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도 |
| CN116356000A (zh) | 2016-03-02 | 2023-06-30 | 牛津纳米孔科技公开有限公司 | 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔 |
| EP4397970A3 (en) | 2016-04-06 | 2024-10-09 | Oxford Nanopore Technologies PLC | Mutant pore |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| EP3645552B1 (en) | 2017-06-30 | 2023-06-28 | Vib Vzw | Novel protein pores |
-
2013
- 2013-03-15 WO PCT/GB2013/050667 patent/WO2013153359A1/en active Application Filing
- 2013-03-15 JP JP2015505016A patent/JP6271505B2/ja active Active
- 2013-03-15 EP EP13711464.1A patent/EP2836506B1/en active Active
- 2013-03-15 KR KR1020147031143A patent/KR102083695B1/ko active IP Right Grant
- 2013-03-15 CN CN201380030527.2A patent/CN104350067A/zh active Pending
- 2013-03-15 US US14/391,660 patent/US9777049B2/en active Active
- 2013-03-15 BR BR112014025157-6A patent/BR112014025157B1/pt active IP Right Grant
- 2013-03-15 CA CA2869546A patent/CA2869546C/en active Active
- 2013-03-15 CN CN202011579254.5A patent/CN112646019B/zh active Active
- 2013-03-15 AU AU2013246702A patent/AU2013246702B2/en active Active
- 2013-03-15 EP EP17157441.1A patent/EP3192804B1/en active Active
-
2017
- 2017-08-31 US US15/692,498 patent/US10882889B2/en active Active
- 2017-12-27 JP JP2017250647A patent/JP2018075025A/ja active Pending
-
2020
- 2020-12-01 US US17/108,536 patent/US11845780B2/en active Active
-
2023
- 2023-11-08 US US18/504,590 patent/US20240199711A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015514128A5 (ja) | ||
| JP2022046503A5 (ja) | ||
| JP2017535256A5 (ja) | ||
| JP2022177005A5 (ja) | ||
| JP2019505521A5 (ja) | ||
| HRP20200370T1 (hr) | Biokemijski stabilizirano hiv-1 trimer cjepivo | |
| HRP20210792T1 (hr) | Imunosupresivna sredstva i njihova uporaba u terapiji | |
| JP2014193932A5 (ja) | ||
| JP2012115277A5 (ja) | ||
| JP2013523179A5 (ja) | ||
| JP2009225798A5 (ja) | ||
| HRP20150479T1 (hr) | Multimeriäśna multiepitopska cjepiva protiv influence | |
| RU2012102701A (ru) | Антигенные taу-пептиды и их применения | |
| JP2014506575A5 (ja) | ||
| RU2016119152A (ru) | Стабильные и растворимые антитела, ингибирующие vegf | |
| NZ616761A (en) | Amino acid sequences directed against il-17a, il-17f and/or il17-a/f and polypeptides comprising the same | |
| JP2010535026A5 (ja) | ||
| JP2015524403A5 (ja) | ||
| JP2013539361A5 (ja) | ||
| JP2010532978A5 (ja) | ||
| JP2010065046A5 (ja) | ||
| JP2017509335A5 (ja) | ||
| JP2013507132A5 (ja) | ||
| JP2013520426A5 (ja) | ||
| Zhao et al. | Preparation of high water flux and antifouling RO membranes using a novel diacyl chloride monomer with a phosphonate group |