JP2015510392A5 - - Google Patents
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- JP2015510392A5 JP2015510392A5 JP2014548993A JP2014548993A JP2015510392A5 JP 2015510392 A5 JP2015510392 A5 JP 2015510392A5 JP 2014548993 A JP2014548993 A JP 2014548993A JP 2014548993 A JP2014548993 A JP 2014548993A JP 2015510392 A5 JP2015510392 A5 JP 2015510392A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- polypeptide
- recombinant cell
- cell according
- recombinant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004027 cell Anatomy 0.000 claims 50
- 229920001184 polypeptide Polymers 0.000 claims 24
- 102000004196 processed proteins & peptides Human genes 0.000 claims 24
- 108090000765 processed proteins & peptides Proteins 0.000 claims 24
- 108020004707 nucleic acids Proteins 0.000 claims 18
- 102000039446 nucleic acids Human genes 0.000 claims 18
- 150000007523 nucleic acids Chemical class 0.000 claims 18
- 101150064873 ispA gene Proteins 0.000 claims 16
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 claims 12
- KJTLQQUUPVSXIM-ZCFIWIBFSA-N (R)-mevalonic acid Chemical compound OCC[C@](O)(C)CC(O)=O KJTLQQUUPVSXIM-ZCFIWIBFSA-N 0.000 claims 10
- KJTLQQUUPVSXIM-UHFFFAOYSA-N DL-mevalonic acid Natural products OCCC(O)(C)CC(O)=O KJTLQQUUPVSXIM-UHFFFAOYSA-N 0.000 claims 10
- 230000037361 pathway Effects 0.000 claims 9
- 108010075483 isoprene synthase Proteins 0.000 claims 8
- 210000005253 yeast cell Anatomy 0.000 claims 7
- 101100397224 Bacillus subtilis (strain 168) isp gene Proteins 0.000 claims 6
- 101100052502 Shigella flexneri yciB gene Proteins 0.000 claims 6
- 230000001580 bacterial effect Effects 0.000 claims 6
- 101710094045 1-deoxy-D-xylulose-5-phosphate synthase Proteins 0.000 claims 5
- 241000193422 Bacillus lentus Species 0.000 claims 3
- 241000195493 Cryptophyta Species 0.000 claims 3
- 241000196324 Embryophyta Species 0.000 claims 2
- 244000166124 Eucalyptus globulus Species 0.000 claims 2
- 241000233866 Fungi Species 0.000 claims 2
- 108010065958 Isopentenyl-diphosphate Delta-isomerase Proteins 0.000 claims 2
- 102100027665 Isopentenyl-diphosphate Delta-isomerase 1 Human genes 0.000 claims 2
- 244000046146 Pueraria lobata Species 0.000 claims 2
- 235000010575 Pueraria lobata Nutrition 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 230000005714 functional activity Effects 0.000 claims 2
- 230000002538 fungal effect Effects 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 238000003786 synthesis reaction Methods 0.000 claims 2
- 238000011144 upstream manufacturing Methods 0.000 claims 2
- 108020005544 Antisense RNA Proteins 0.000 claims 1
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims 1
- 241000193752 Bacillus circulans Species 0.000 claims 1
- 241001328122 Bacillus clausii Species 0.000 claims 1
- 241000193749 Bacillus coagulans Species 0.000 claims 1
- 241000006382 Bacillus halodurans Species 0.000 claims 1
- 241000194108 Bacillus licheniformis Species 0.000 claims 1
- 241000194107 Bacillus megaterium Species 0.000 claims 1
- 235000014469 Bacillus subtilis Nutrition 0.000 claims 1
- 241000193388 Bacillus thuringiensis Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 241000149420 Bothrometopus brevis Species 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- 241000195628 Chlorophyta Species 0.000 claims 1
- 241000199914 Dinophyceae Species 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000195620 Euglena Species 0.000 claims 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims 1
- 102000004286 Hydroxymethylglutaryl CoA Reductases Human genes 0.000 claims 1
- 108090000895 Hydroxymethylglutaryl CoA Reductases Proteins 0.000 claims 1
- 102000002284 Hydroxymethylglutaryl-CoA Synthase Human genes 0.000 claims 1
- 108010000775 Hydroxymethylglutaryl-CoA synthase Proteins 0.000 claims 1
- 229930194542 Keto Natural products 0.000 claims 1
- 241000194109 Paenibacillus lautus Species 0.000 claims 1
- 241000235061 Pichia sp. Species 0.000 claims 1
- 241000219000 Populus Species 0.000 claims 1
- 241000168036 Populus alba Species 0.000 claims 1
- 241000218982 Populus nigra Species 0.000 claims 1
- 241001600128 Populus tremula x Populus alba Species 0.000 claims 1
- 240000004923 Populus tremuloides Species 0.000 claims 1
- 235000011263 Populus tremuloides Nutrition 0.000 claims 1
- 241000218976 Populus trichocarpa Species 0.000 claims 1
- 241000157935 Promicromonospora citrea Species 0.000 claims 1
- 241000168225 Pseudomonas alcaligenes Species 0.000 claims 1
- 241000589774 Pseudomonas sp. Species 0.000 claims 1
- 241000219780 Pueraria Species 0.000 claims 1
- 241000913776 Pueraria montana Species 0.000 claims 1
- 241000206572 Rhodophyta Species 0.000 claims 1
- 241000235088 Saccharomyces sp. Species 0.000 claims 1
- 241000720795 Schizosaccharomyces sp. Species 0.000 claims 1
- 241000063122 Streptacidiphilus griseus Species 0.000 claims 1
- 241000187747 Streptomyces Species 0.000 claims 1
- 241000187759 Streptomyces albus Species 0.000 claims 1
- 241000187432 Streptomyces coelicolor Species 0.000 claims 1
- 241000187398 Streptomyces lividans Species 0.000 claims 1
- 102000002932 Thiolase Human genes 0.000 claims 1
- 108060008225 Thiolase Proteins 0.000 claims 1
- OJFDKHTZOUZBOS-CITAKDKDSA-N acetoacetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 OJFDKHTZOUZBOS-CITAKDKDSA-N 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 claims 1
- 239000003184 complementary RNA Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 125000000468 ketone group Chemical group 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- CBIDRCWHNCKSTO-UHFFFAOYSA-N prenyl diphosphate Chemical compound CC(C)=CCO[P@](O)(=O)OP(O)(O)=O CBIDRCWHNCKSTO-UHFFFAOYSA-N 0.000 claims 1
- 230000004952 protein activity Effects 0.000 claims 1
- 230000002797 proteolythic effect Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 239000002699 waste material Substances 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161580163P | 2011-12-23 | 2011-12-23 | |
| US61/580,163 | 2011-12-23 | ||
| US201261639855P | 2012-04-27 | 2012-04-27 | |
| US61/639,855 | 2012-04-27 | ||
| PCT/US2012/071518 WO2013096925A1 (en) | 2011-12-23 | 2012-12-21 | Enhanced production of isoprene using host cells having decreased ispa activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015510392A JP2015510392A (ja) | 2015-04-09 |
| JP2015510392A5 true JP2015510392A5 (enExample) | 2016-02-12 |
Family
ID=47522963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014548993A Pending JP2015510392A (ja) | 2011-12-23 | 2012-12-21 | 低減したｉｓｐａ活性を有する宿主細胞を用いるイソプレン産生増強法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8975051B2 (enExample) |
| EP (1) | EP2794863A1 (enExample) |
| JP (1) | JP2015510392A (enExample) |
| CN (1) | CN104245927A (enExample) |
| AU (1) | AU2012358149A1 (enExample) |
| BR (1) | BR112014014947A2 (enExample) |
| CA (1) | CA2859885A1 (enExample) |
| MX (1) | MX2014007623A (enExample) |
| SG (1) | SG11201403163QA (enExample) |
| WO (1) | WO2013096925A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
| CA2880962C (en) * | 2012-08-17 | 2019-07-30 | Evolva Sa | Increased production of terpenes and terpenoids |
| WO2015083769A1 (ja) * | 2013-12-04 | 2015-06-11 | 国立研究開発法人理化学研究所 | 光合成のみによるポリヒドロキシアルカン酸の生産 |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| JP2018014960A (ja) * | 2016-07-29 | 2018-02-01 | 横浜ゴム株式会社 | 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| WO2018208116A1 (ko) * | 2017-05-11 | 2018-11-15 | 경상대학교산학협력단 | 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법 |
| EP3623480A4 (en) | 2017-05-11 | 2021-02-24 | Industry-Academic Cooperation Foundation Gyeongsang National University | COMPOSITION OF MARKER FOR SELECTING A LIVING MODIFIED ORGANISM, LIVING MODIFIED ORGANISM AND PROCESS OF TRANSFORMATION |
| CN110317765B (zh) * | 2018-03-29 | 2020-11-20 | 中国科学院天津工业生物技术研究所 | 一种高产香叶醇葡萄糖苷的大肠杆菌表达菌株及其应用 |
| CN113366009B (zh) * | 2018-11-27 | 2025-02-11 | 科纳科学控股有限公司 | 用于生物合成大麻素的双向多酶支架 |
| US12351850B2 (en) * | 2020-04-30 | 2025-07-08 | Sutro Biopharma, Inc. | Methods of producing full-length antibodies using E. coli |
| CN112553134B (zh) * | 2020-12-30 | 2022-09-06 | 江南大学 | 一种在枯草芽孢杆菌中表达α-淀粉酶的方法 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19629568C1 (de) | 1996-07-15 | 1998-01-08 | Fraunhofer Ges Forschung | Verfahren zur Herstellung von Isopren |
| US20050287655A1 (en) | 2002-05-10 | 2005-12-29 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing mevalonic acid |
| AU2003287028B2 (en) | 2002-10-04 | 2008-09-04 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| RU2004124226A (ru) | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
| CA2651747C (en) * | 2006-05-26 | 2017-10-24 | Amyris Biotechnologies, Inc. | Production of isoprenoids |
| US7947478B2 (en) | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| JP4986547B2 (ja) | 2006-09-04 | 2012-07-25 | 株式会社Ａｄｅｋａ | 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法 |
| BRPI0823506A8 (pt) | 2007-12-13 | 2018-05-15 | Danisco Us Inc Genencor Div | composições e métodos para a produção de isoprene |
| WO2009100231A2 (en) | 2008-02-06 | 2009-08-13 | The Regents Of The University Of California | Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi |
| JP2011518564A (ja) | 2008-04-23 | 2011-06-30 | ダニスコ・ユーエス・インク | 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 |
| US7632995B2 (en) | 2008-05-09 | 2009-12-15 | Monsanto Technology Llc | Plants and seeds of hybrid corn variety CH852179 |
| EP2310490B1 (en) | 2008-07-02 | 2019-01-02 | Danisco US Inc. | Methods for producing isoprene |
| BRPI0918936A2 (pt) | 2008-09-15 | 2019-09-24 | Danisco Us Inc | conversão de derivados de prenila em isopreno |
| MY156562A (en) | 2008-09-15 | 2016-02-26 | Danisco Us Inc | Increased isoprene production using the archaeal lower mevalonate pathway |
| CA2737223A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| WO2010031079A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Systems using cell culture for production of isoprene |
| CN102333866B (zh) | 2008-12-30 | 2015-04-29 | 丹尼斯科美国公司 | 生产异戊二烯和共同产物的方法 |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| EP2421966A2 (en) | 2009-04-23 | 2012-02-29 | Danisco US Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| JP4760951B2 (ja) | 2009-05-08 | 2011-08-31 | トヨタ自動車株式会社 | ブタノール生産能を有する組換え微生物及びブタノールの製造方法 |
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| TWI434921B (zh) | 2009-06-17 | 2014-04-21 | Danisco Us Inc | 從生物異戊二烯組合物製造燃料成分之方法及系統 |
| CA2773675A1 (en) | 2009-09-15 | 2011-03-24 | Sapphire Energy, Inc. | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| CN102884028B (zh) | 2009-12-18 | 2015-08-19 | 丹尼斯科美国公司 | 从可再生资源中纯化异戊二烯 |
| AU2010336342B2 (en) * | 2009-12-23 | 2015-02-26 | Danisco Us Inc. | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL) |
| US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
| JP2014502148A (ja) | 2010-10-27 | 2014-01-30 | ダニスコ・ユーエス・インク | イソプレン生産の向上を目的としたイソプレン合成酵素変異体 |
-
2012
- 2012-12-21 US US13/725,929 patent/US8975051B2/en not_active Expired - Fee Related
- 2012-12-21 CN CN201280070444.1A patent/CN104245927A/zh active Pending
- 2012-12-21 MX MX2014007623A patent/MX2014007623A/es unknown
- 2012-12-21 SG SG11201403163QA patent/SG11201403163QA/en unknown
- 2012-12-21 CA CA2859885A patent/CA2859885A1/en not_active Abandoned
- 2012-12-21 BR BR112014014947-0A patent/BR112014014947A2/pt not_active Application Discontinuation
- 2012-12-21 EP EP12813234.7A patent/EP2794863A1/en not_active Withdrawn
- 2012-12-21 JP JP2014548993A patent/JP2015510392A/ja active Pending
- 2012-12-21 WO PCT/US2012/071518 patent/WO2013096925A1/en not_active Ceased
- 2012-12-21 AU AU2012358149A patent/AU2012358149A1/en not_active Abandoned
-
2015
- 2015-01-09 US US14/593,860 patent/US9388431B2/en not_active Expired - Fee Related
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