CA2859885A1 - Enhanced production of isoprene using host cells having decreased ispa activity - Google Patents
Enhanced production of isoprene using host cells having decreased ispa activity Download PDFInfo
- Publication number
- CA2859885A1 CA2859885A1 CA2859885A CA2859885A CA2859885A1 CA 2859885 A1 CA2859885 A1 CA 2859885A1 CA 2859885 A CA2859885 A CA 2859885A CA 2859885 A CA2859885 A CA 2859885A CA 2859885 A1 CA2859885 A1 CA 2859885A1
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- Prior art keywords
- cells
- polypeptide
- ispa
- isoprene
- nucleic acids
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01001—Dimethylallyltranstransferase (2.5.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03027—Isoprene synthase (4.2.3.27)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| PCT/US2012/071518 WO2013096925A1 (en) | 2011-12-23 | 2012-12-21 | Enhanced production of isoprene using host cells having decreased ispa activity |
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| CA2880962C (en) * | 2012-08-17 | 2019-07-30 | Evolva Sa | Increased production of terpenes and terpenoids |
| WO2015083769A1 (ja) * | 2013-12-04 | 2015-06-11 | 国立研究開発法人理化学研究所 | 光合成のみによるポリヒドロキシアルカン酸の生産 |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| JP2018014960A (ja) * | 2016-07-29 | 2018-02-01 | 横浜ゴム株式会社 | 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| WO2018208116A1 (ko) * | 2017-05-11 | 2018-11-15 | 경상대학교산학협력단 | 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법 |
| EP3623480A4 (en) | 2017-05-11 | 2021-02-24 | Industry-Academic Cooperation Foundation Gyeongsang National University | COMPOSITION OF MARKER FOR SELECTING A LIVING MODIFIED ORGANISM, LIVING MODIFIED ORGANISM AND PROCESS OF TRANSFORMATION |
| CN110317765B (zh) * | 2018-03-29 | 2020-11-20 | 中国科学院天津工业生物技术研究所 | 一种高产香叶醇葡萄糖苷的大肠杆菌表达菌株及其应用 |
| CN113366009B (zh) * | 2018-11-27 | 2025-02-11 | 科纳科学控股有限公司 | 用于生物合成大麻素的双向多酶支架 |
| US12351850B2 (en) * | 2020-04-30 | 2025-07-08 | Sutro Biopharma, Inc. | Methods of producing full-length antibodies using E. coli |
| CN112553134B (zh) * | 2020-12-30 | 2022-09-06 | 江南大学 | 一种在枯草芽孢杆菌中表达α-淀粉酶的方法 |
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| US20050287655A1 (en) | 2002-05-10 | 2005-12-29 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing mevalonic acid |
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| RU2004124226A (ru) | 2004-08-10 | 2006-01-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Использование фосфокетолазы для продукции полезных метаболитов |
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| US7947478B2 (en) | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| JP4986547B2 (ja) | 2006-09-04 | 2012-07-25 | 株式会社Ａｄｅｋａ | 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法 |
| BRPI0823506A8 (pt) | 2007-12-13 | 2018-05-15 | Danisco Us Inc Genencor Div | composições e métodos para a produção de isoprene |
| WO2009100231A2 (en) | 2008-02-06 | 2009-08-13 | The Regents Of The University Of California | Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi |
| JP2011518564A (ja) | 2008-04-23 | 2011-06-30 | ダニスコ・ユーエス・インク | 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体 |
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| CA2737223A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Reduction of carbon dioxide emission during isoprene production by fermentation |
| WO2010031079A1 (en) | 2008-09-15 | 2010-03-18 | Danisco Us Inc. | Systems using cell culture for production of isoprene |
| CN102333866B (zh) | 2008-12-30 | 2015-04-29 | 丹尼斯科美国公司 | 生产异戊二烯和共同产物的方法 |
| US20100297749A1 (en) | 2009-04-21 | 2010-11-25 | Sapphire Energy, Inc. | Methods and systems for biofuel production |
| EP2421966A2 (en) | 2009-04-23 | 2012-02-29 | Danisco US Inc. | Three-dimensional structure of isoprene synthase and its use thereof for generating variants |
| JP4760951B2 (ja) | 2009-05-08 | 2011-08-31 | トヨタ自動車株式会社 | ブタノール生産能を有する組換え微生物及びブタノールの製造方法 |
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| TWI434921B (zh) | 2009-06-17 | 2014-04-21 | Danisco Us Inc | 從生物異戊二烯組合物製造燃料成分之方法及系統 |
| CA2773675A1 (en) | 2009-09-15 | 2011-03-24 | Sapphire Energy, Inc. | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| CN102884028B (zh) | 2009-12-18 | 2015-08-19 | 丹尼斯科美国公司 | 从可再生资源中纯化异戊二烯 |
| AU2010336342B2 (en) * | 2009-12-23 | 2015-02-26 | Danisco Us Inc. | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL) |
| US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
| JP2014502148A (ja) | 2010-10-27 | 2014-01-30 | ダニスコ・ユーエス・インク | イソプレン生産の向上を目的としたイソプレン合成酵素変異体 |
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- 2012-12-21 CN CN201280070444.1A patent/CN104245927A/zh active Pending
- 2012-12-21 MX MX2014007623A patent/MX2014007623A/es unknown
- 2012-12-21 SG SG11201403163QA patent/SG11201403163QA/en unknown
- 2012-12-21 CA CA2859885A patent/CA2859885A1/en not_active Abandoned
- 2012-12-21 BR BR112014014947-0A patent/BR112014014947A2/pt not_active Application Discontinuation
- 2012-12-21 EP EP12813234.7A patent/EP2794863A1/en not_active Withdrawn
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- 2012-12-21 WO PCT/US2012/071518 patent/WO2013096925A1/en not_active Ceased
- 2012-12-21 AU AU2012358149A patent/AU2012358149A1/en not_active Abandoned
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2015
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| Publication number | Publication date |
|---|---|
| SG11201403163QA (en) | 2014-09-26 |
| EP2794863A1 (en) | 2014-10-29 |
| US9388431B2 (en) | 2016-07-12 |
| US20130164808A1 (en) | 2013-06-27 |
| JP2015510392A (ja) | 2015-04-09 |
| US8975051B2 (en) | 2015-03-10 |
| CN104245927A (zh) | 2014-12-24 |
| US20150218590A1 (en) | 2015-08-06 |
| AU2012358149A1 (en) | 2014-07-03 |
| MX2014007623A (es) | 2015-02-04 |
| WO2013096925A1 (en) | 2013-06-27 |
| BR112014014947A2 (pt) | 2020-10-27 |
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