CN104245927A - 使用具有降低的ispa活性的宿主细胞提高异戊二烯的产量 - Google Patents

使用具有降低的ispa活性的宿主细胞提高异戊二烯的产量 Download PDF

Info

Publication number
CN104245927A
CN104245927A CN201280070444.1A CN201280070444A CN104245927A CN 104245927 A CN104245927 A CN 104245927A CN 201280070444 A CN201280070444 A CN 201280070444A CN 104245927 A CN104245927 A CN 104245927A
Authority
CN
China
Prior art keywords
cell
polypeptide
ispa
isoprene
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280070444.1A
Other languages
English (en)
Chinese (zh)
Inventor
J·C·麦考利夫
R·E·穆尔
A·T·尼尔森
C·M·派瑞斯
D·V·瓦维林
D·H·韦尔斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Goodyear Tire and Rubber Co
Danisco USA Inc
Original Assignee
Goodyear Tire and Rubber Co
Danisco USA Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Goodyear Tire and Rubber Co, Danisco USA Inc filed Critical Goodyear Tire and Rubber Co
Publication of CN104245927A publication Critical patent/CN104245927A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01001—Dimethylallyltranstransferase (2.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280070444.1A 2011-12-23 2012-12-21 使用具有降低的ispa活性的宿主细胞提高异戊二烯的产量 Pending CN104245927A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161580163P 2011-12-23 2011-12-23
US61/580,163 2011-12-23
US201261639855P 2012-04-27 2012-04-27
US61/639,855 2012-04-27
PCT/US2012/071518 WO2013096925A1 (en) 2011-12-23 2012-12-21 Enhanced production of isoprene using host cells having decreased ispa activity

Publications (1)

Publication Number Publication Date
CN104245927A true CN104245927A (zh) 2014-12-24

Family

ID=47522963

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280070444.1A Pending CN104245927A (zh) 2011-12-23 2012-12-21 使用具有降低的ispa活性的宿主细胞提高异戊二烯的产量

Country Status (10)

Country Link
US (2) US8975051B2 (enExample)
EP (1) EP2794863A1 (enExample)
JP (1) JP2015510392A (enExample)
CN (1) CN104245927A (enExample)
AU (1) AU2012358149A1 (enExample)
BR (1) BR112014014947A2 (enExample)
CA (1) CA2859885A1 (enExample)
MX (1) MX2014007623A (enExample)
SG (1) SG11201403163QA (enExample)
WO (1) WO2013096925A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317765A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种高产香叶醇葡萄糖苷的大肠杆菌表达菌株及其应用
CN112553134A (zh) * 2020-12-30 2021-03-26 江南大学 一种在枯草芽孢杆菌中表达α-淀粉酶的方法

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
EP2885420B1 (en) 2012-08-17 2018-12-05 Evolva SA Increased production of terpenes and terpenoids
JP6492011B2 (ja) * 2013-12-04 2019-03-27 国立研究開発法人理化学研究所 光合成のみによるポリヒドロキシアルカン酸の生産
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
JP2018014960A (ja) * 2016-07-29 2018-02-01 横浜ゴム株式会社 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11549117B2 (en) 2017-05-11 2023-01-10 Industry-Academic Cooperation Foundation Gyeongsang National University Marker composition for selecting living modified organism, living modified organism, and transformation method
WO2018208116A1 (ko) * 2017-05-11 2018-11-15 경상대학교산학협력단 형질전환 생물체 선별용 마커 조성물, 형질전환 생물체 및 형질전환 방법
IL283384B2 (en) * 2018-11-27 2025-07-01 Khona Scient Inc Bidirectional multi-enzymatic scaffolds for cannabinoid biosynthesis
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007140339A2 (en) * 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production of isoprenoids
CN102027124A (zh) * 2007-12-13 2011-04-20 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
US20110159557A1 (en) * 2009-12-23 2011-06-30 Danisco Us Inc. Compositions and methods of pgl for the increased production of isoprene

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
EP1510583A1 (en) 2002-05-10 2005-03-02 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
ATE493490T1 (de) 2002-10-04 2011-01-15 Du Pont Verfahren zur biologischen herstellung von 1,3- propandiol mit hoher ausbeute
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
US20110045563A1 (en) 2008-02-06 2011-02-24 The Regents Of The University Of California Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi
BRPI0911667A2 (pt) 2008-04-23 2019-04-24 Danisco Us Inc variante de sintase de isopreno para produção microbiana aprimorada de isopreno
US7632995B2 (en) 2008-05-09 2009-12-15 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH852179
AU2009266938B2 (en) 2008-07-02 2013-10-17 Danisco Us Inc. Compositions and methods for producing isoprene free of C5 hydrocarbons under decoupling conditions and/or safe operating ranges
EP2340312A2 (en) 2008-09-15 2011-07-06 Danisco US Inc. Conversion of prenyl derivatives to isoprene
SG169652A1 (en) 2008-09-15 2011-04-29 Danisco Us Inc Reduction of carbon dioxide emission during isoprene production by fermentation
MY156562A (en) 2008-09-15 2016-02-26 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
DK2344629T3 (en) 2008-09-15 2018-03-05 Danisco Us Inc SYSTEMS USING CELL CULTURE TO MAKE ISOPRENE
EP2382312A2 (en) 2008-12-30 2011-11-02 Danisco US Inc. Methods of producing isoprene and a co-product
US20100297749A1 (en) 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
SG10201501879PA (en) 2009-04-23 2015-05-28 Danisco Us Inc Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
WO2011034863A1 (en) 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
WO2011075534A2 (en) 2009-12-18 2011-06-23 Danisco Us Inc. Purification of isoprene from renewable resources
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
BR112013010551A8 (pt) 2010-10-27 2018-07-03 Danisco Us Inc variantes de isopreno sintase para produção aprimorada de isopreno

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007140339A2 (en) * 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production of isoprenoids
CN102027124A (zh) * 2007-12-13 2011-04-20 丹尼斯科美国公司 用于生产异戊二烯的组合物和方法
US20110159557A1 (en) * 2009-12-23 2011-06-30 Danisco Us Inc. Compositions and methods of pgl for the increased production of isoprene

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DOUGLAS J. PITERA 等: "Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli", 《METABOLIC ENGINEERING》, vol. 9, no. 2, 31 March 2007 (2007-03-31) *
SHINGO FUJISAKI等: "Disruption of the Structural Gene for Farnesyl Diphosphate Synthase in Escherichia coli", 《J. BIOCHEM.》, vol. 137, no. 3, 31 March 2005 (2005-03-31) *
郝宏蕾 等: "类异戊二烯的生物合成及调控", 《浙江大学学报（农业与生命科学版）》, vol. 28, no. 2, 31 December 2002 (2002-12-31) *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110317765A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种高产香叶醇葡萄糖苷的大肠杆菌表达菌株及其应用
CN110317765B (zh) * 2018-03-29 2020-11-20 中国科学院天津工业生物技术研究所 一种高产香叶醇葡萄糖苷的大肠杆菌表达菌株及其应用
CN112553134A (zh) * 2020-12-30 2021-03-26 江南大学 一种在枯草芽孢杆菌中表达α-淀粉酶的方法
CN112553134B (zh) * 2020-12-30 2022-09-06 江南大学 一种在枯草芽孢杆菌中表达α-淀粉酶的方法

Also Published As

Publication number Publication date
US20150218590A1 (en) 2015-08-06
EP2794863A1 (en) 2014-10-29
MX2014007623A (es) 2015-02-04
AU2012358149A1 (en) 2014-07-03
WO2013096925A1 (en) 2013-06-27
CA2859885A1 (en) 2013-06-27
BR112014014947A2 (pt) 2020-10-27
US20130164808A1 (en) 2013-06-27
US8975051B2 (en) 2015-03-10
US9388431B2 (en) 2016-07-12
SG11201403163QA (en) 2014-09-26
JP2015510392A (ja) 2015-04-09

Similar Documents

Publication Publication Date Title
US9388431B2 (en) Enhanced production of isoprene using host cells having decreased ispA activity
US10975394B2 (en) Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids
JP6639606B2 (ja) メバロン酸、イソプレノイド前駆体、及びイソプレンの産生におけるホスホケトラーゼの使用
US11371035B2 (en) Phosphoketolases for improved production of acetyl coenzyme A-derived metabolites, isoprene, isoprenoid precursors, and isoprenoid
CN103764814B (zh) 利用编码具有硫解酶、hmg-coa合酶和hmg-coa还原酶酶活性的多肽的基因产生甲羟戊酸、异戊二烯和类异戊二烯
US8865442B2 (en) Production of isoprene under reduced oxygen inlet levels

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20141224

WD01 Invention patent application deemed withdrawn after publication