JP2015154787A5 - - Google Patents

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JP2015154787A5
JP2015154787A5 JP2015111259A JP2015111259A JP2015154787A5 JP 2015154787 A5 JP2015154787 A5 JP 2015154787A5 JP 2015111259 A JP2015111259 A JP 2015111259A JP 2015111259 A JP2015111259 A JP 2015111259A JP 2015154787 A5 JP2015154787 A5 JP 2015154787A5
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JP
Japan
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polymerase
seq
amino acid
domain
chimeric
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JP2015111259A
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Japanese (ja)
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JP2015154787A (ja
JP6150847B2 (ja
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JP2015111259A 2008-11-03 2015-06-01 キメラdnaポリメラーゼ Active JP6150847B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03
US61/110,862 2008-11-03

Related Parent Applications (1)

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JP2011534889A Division JP2012507986A (ja) 2008-11-03 2009-11-03 キメラdnaポリメラーゼ

Related Child Applications (1)

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JP2016219403A Division JP2017029172A (ja) 2008-11-03 2016-11-10 キメラdnaポリメラーゼ

Publications (3)

Publication Number Publication Date
JP2015154787A JP2015154787A (ja) 2015-08-27
JP2015154787A5 true JP2015154787A5 (enExample) 2016-02-18
JP6150847B2 JP6150847B2 (ja) 2017-06-21

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ID=42226349

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JP2011534889A Withdrawn JP2012507986A (ja) 2008-11-03 2009-11-03 キメラdnaポリメラーゼ
JP2015111259A Active JP6150847B2 (ja) 2008-11-03 2015-06-01 キメラdnaポリメラーゼ
JP2016219403A Pending JP2017029172A (ja) 2008-11-03 2016-11-10 キメラdnaポリメラーゼ

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JP2011534889A Withdrawn JP2012507986A (ja) 2008-11-03 2009-11-03 キメラdnaポリメラーゼ

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JP2016219403A Pending JP2017029172A (ja) 2008-11-03 2016-11-10 キメラdnaポリメラーゼ

Country Status (7)

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US (2) US9023633B2 (enExample)
EP (2) EP2352818B1 (enExample)
JP (3) JP2012507986A (enExample)
CN (1) CN102257136A (enExample)
CA (1) CA2742593C (enExample)
ES (2) ES2571446T3 (enExample)
WO (1) WO2010062776A2 (enExample)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
CN102245761A (zh) 2008-11-03 2011-11-16 卡帕生物系统 修饰a型dna聚合酶
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
CN102392000A (zh) * 2011-11-07 2012-03-28 李志茹 一种耐高温Pyrolobus聚合酶及其高效表达质粒和应用
TWI487789B (zh) 2012-12-24 2015-06-11 Ind Tech Res Inst 單離的脫氧核糖核酸聚合酶、套組與其應用
CN104250641B (zh) * 2013-06-28 2017-09-01 北京福安华生物科技有限公司 一种高保真dna聚合酶及其制备和应用
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
WO2015200693A1 (en) * 2014-06-27 2015-12-30 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
JP6594955B2 (ja) * 2014-08-27 2019-10-23 ニユー・イングランド・バイオレイブス・インコーポレイテツド シントン形成
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
JP6619152B2 (ja) * 2015-05-08 2019-12-11 国立研究開発法人産業技術総合研究所 フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するキメラタンパク質
CN106867978A (zh) * 2015-12-10 2017-06-20 江苏众红生物工程创药研究院有限公司 一种重组高保真dna聚合酶及其编码基因和表达方法
JP6980375B2 (ja) * 2015-12-11 2021-12-15 東洋紡株式会社 核酸増幅用試薬及び核酸増幅法
JP6969089B2 (ja) * 2015-12-11 2021-11-24 東洋紡株式会社 核酸増幅用組成物及び核酸増幅法
LT3402880T (lt) 2016-01-15 2024-03-12 Thermo Fisher Scientific Baltics Uab Termofiliniai dnr polimerazės mutantai
US10323243B2 (en) * 2016-01-19 2019-06-18 Board Of Regents, The University Of Texas System Thermostable reverse transcriptase
WO2017201315A1 (en) 2016-05-18 2017-11-23 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
FR3052462A1 (fr) * 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
CN106011100A (zh) * 2016-08-08 2016-10-12 吴江近岸蛋白质科技有限公司 一种Pfu DNA聚合酶及其制备方法
WO2018118997A2 (en) * 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN106754816B (zh) * 2017-02-24 2020-10-27 依科赛生物科技(太仓)有限公司 一种高保真快速扩增融合酶及其制备方法
US11618891B2 (en) 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
KR102773542B1 (ko) 2017-06-30 2025-02-25 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
JP2020530267A (ja) 2017-06-30 2020-10-22 コデクシス, インコーポレイテッド T7 rnaポリメラーゼバリアント
EP4530346A3 (en) * 2017-07-12 2025-06-11 Genecast Co., Ltd. Dna polymerase with increased gene mutation specificity and pcr buffer composition for increasing activity thereof
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
WO2019143622A1 (en) 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
WO2020047695A1 (zh) * 2018-09-03 2020-03-12 深圳华大生命科学研究院 重组型kod聚合酶
JP7515413B2 (ja) * 2018-10-31 2024-07-12 イラミーナ インコーポレーテッド ポリメラーゼ、組成物、および使用方法
EP3891275A2 (en) 2018-12-05 2021-10-13 Illumina, Inc. Polymerases, compositions, and methods of use
CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
ES2980651T3 (es) * 2019-01-09 2024-10-02 3Cr Bioscience Ltd Método de detección de una secuencia de ácido nucleico
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
KR20220029708A (ko) 2019-06-28 2022-03-08 퀀텀-에스아이 인코포레이티드 서열분석 반응을 위한 중합 효소
WO2021127848A1 (zh) * 2019-12-23 2021-07-01 深圳华大生命科学研究院 嵌合dna聚合酶及其应用
EP4103747A4 (en) * 2020-02-15 2024-03-13 Agency for Science, Technology and Research METHOD FOR AMPLIFICATION OF A NUCLEIC ACID
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465B (zh) * 2021-09-23 2026-07-10 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
WO2023082266A1 (zh) * 2021-11-15 2023-05-19 深圳华大生命科学研究院 嵌合dna聚合酶及其应用
CN119736271B (zh) * 2024-12-27 2026-03-20 安序源生物科技(深圳)有限公司 一种phi29 DNA聚合酶突变体及其用途

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5545552A (en) 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5948663A (en) 1990-12-03 1999-09-07 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5866395A (en) 1990-12-03 1999-02-02 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
DE69232511T2 (de) * 1991-12-18 2002-10-24 New England Biolabs, Inc. Gereinigte thermostabile DNS-Polymerase aus Pyrococcus-Arten
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
JP3132624B2 (ja) * 1994-05-09 2001-02-05 東洋紡績株式会社 超好熱始原菌由来のdnaポリメラーゼ遺伝子およびその用途
US6033859A (en) 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
WO1998033900A1 (en) 1997-01-31 1998-08-06 Japan Science And Technology Corporation Method and apparatus for predicting protein function site, method for improving protein function, and function-improved protein
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
IL141481A0 (en) 1998-08-18 2002-03-10 Dermtech Int Method for detection of biological factors in epidermis
GB9921318D0 (en) * 1999-09-09 1999-11-10 Kristensen Tom Chimeric molecules
AU1239901A (en) 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
US20050123940A1 (en) 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
WO2001061015A2 (en) * 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
EP1154017B1 (en) 2000-05-11 2010-01-20 Toyo Boseki Kabushiki Kaisha Modified thermostable dna polymerase from pyrococcus kodakarensis
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
WO2002004022A1 (en) 2000-07-12 2002-01-17 Invitrogen Corp High fidelity polymerases and uses thereof
GB2382321A (en) 2001-11-21 2003-05-28 Doyen Medipharm Ltd Process and apparatus for cutting sheets
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
AU2004225520A1 (en) 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
US20040244310A1 (en) 2003-03-28 2004-12-09 Blumberg Marvin R. Data center
US7745188B2 (en) * 2004-05-20 2010-06-29 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Thermostable Y-family polymerases and chimeras
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
WO2007016702A2 (en) 2005-07-29 2007-02-08 Applera Corporation Chimeric polymerases
JP2009521227A (ja) 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
JP2007298879A (ja) 2006-05-02 2007-11-15 C & C:Kk ストロボ装置
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
WO2010062776A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
WO2010062779A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified dna polymerases
JP5328696B2 (ja) 2010-03-01 2013-10-30 淀川ヒューテック株式会社 複層ベルト

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