ES2571446T3 - ADN polimerasas quiméricas - Google Patents

ADN polimerasas quiméricas

Info

Publication number
ES2571446T3
ES2571446T3 ES09829689T ES09829689T ES2571446T3 ES 2571446 T3 ES2571446 T3 ES 2571446T3 ES 09829689 T ES09829689 T ES 09829689T ES 09829689 T ES09829689 T ES 09829689T ES 2571446 T3 ES2571446 T3 ES 2571446T3
Authority
ES
Spain
Prior art keywords
chimeric dna
domain
dna polymerases
polymerase
palm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09829689T
Other languages
English (en)
Inventor
Bjarne Faurholm
Paul Mcewan
William Bourn
Gavin Rush
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kapa Biosystems Inc
Original Assignee
Kapa Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kapa Biosystems Inc filed Critical Kapa Biosystems Inc
Application granted granted Critical
Publication of ES2571446T3 publication Critical patent/ES2571446T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Una ADN polimerasa quimérica que comprende la secuencia aminoacídica de SEQ ID NO: 16, que contiene el dominio N-terminal, el dominio 3'-5' exonucleasa y el dominio de pulgar de polimerasa KOD y el dominio de la palma y los dedos de polimerasa Pfu.
ES09829689T 2008-11-03 2009-11-03 ADN polimerasas quiméricas Active ES2571446T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03
PCT/US2009/063166 WO2010062776A2 (en) 2008-11-03 2009-11-03 Chimeric dna polymerases

Publications (1)

Publication Number Publication Date
ES2571446T3 true ES2571446T3 (es) 2016-05-25

Family

ID=42226349

Family Applications (2)

Application Number Title Priority Date Filing Date
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas
ES09829689T Active ES2571446T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Country Status (8)

Country Link
US (2) US9023633B2 (es)
EP (2) EP2352818B1 (es)
JP (3) JP2012507986A (es)
CN (1) CN102257136A (es)
CA (1) CA2742593C (es)
ES (2) ES2716402T3 (es)
HK (1) HK1216032A1 (es)
WO (1) WO2010062776A2 (es)

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WO2015019658A1 (ja) * 2013-08-06 2015-02-12 東洋紡株式会社 核酸増幅法
CN106536728A (zh) * 2014-06-27 2017-03-22 伊鲁米那股份有限公司 用于核苷酸类似物的改进的掺入的修饰的聚合酶
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JP6594955B2 (ja) * 2014-08-27 2019-10-23 ニユー・イングランド・バイオレイブス・インコーポレイテツド シントン形成
US10329545B2 (en) 2014-11-14 2019-06-25 Illumina, Inc. Polymerases
JP6619152B2 (ja) * 2015-05-08 2019-12-11 国立研究開発法人産業技術総合研究所 フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するキメラタンパク質
CN106867978A (zh) * 2015-12-10 2017-06-20 江苏众红生物工程创药研究院有限公司 一种重组高保真dna聚合酶及其编码基因和表达方法
JP6969089B2 (ja) * 2015-12-11 2021-11-24 東洋紡株式会社 核酸増幅用組成物及び核酸増幅法
JP6980375B2 (ja) * 2015-12-11 2021-12-15 東洋紡株式会社 核酸増幅用試薬及び核酸増幅法
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
WO2017127510A2 (en) 2016-01-19 2017-07-27 Board Of Regents, The University Of Texas System Thermostable reverse transcriptase
US20190176153A1 (en) 2016-05-18 2019-06-13 Roche Sequencing Solutions, Inc. Quantitative real time pcr amplification using an electrowetting-based device
FR3052462A1 (fr) * 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
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CN111417724A (zh) 2017-06-30 2020-07-14 科德克希思公司 T7 rna聚合酶变体
EP3645711A4 (en) 2017-06-30 2021-04-21 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
CN111684064A (zh) 2018-01-19 2020-09-18 生物辐射实验室股份有限公司 突变dna聚合酶
EP3862427A4 (en) * 2018-09-03 2022-04-27 BGI Shenzhen RECOMBINANT KOD POLYMERASE
US11001816B2 (en) 2018-12-05 2021-05-11 Illumina, Inc. Polymerases, compositions, and methods of use
CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
US20220090194A1 (en) * 2019-01-09 2022-03-24 3Cr Bioscience Ltd. Method for detecting a nucleic acid sequence
EP3976771A1 (en) 2019-06-28 2022-04-06 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
WO2021127848A1 (zh) * 2019-12-23 2021-07-01 深圳华大生命科学研究院 嵌合dna聚合酶及其应用
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
EP4435098A1 (en) * 2021-11-15 2024-09-25 BGI Shenzhen Chimeric dna polymerase and use thereof

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Also Published As

Publication number Publication date
EP2352818A2 (en) 2011-08-10
US9388396B2 (en) 2016-07-12
HK1216032A1 (zh) 2016-10-07
EP2352818A4 (en) 2011-10-12
US20120115188A1 (en) 2012-05-10
EP2352818B1 (en) 2016-02-10
CN102257136A (zh) 2011-11-23
CA2742593A1 (en) 2010-06-03
CA2742593C (en) 2020-05-05
JP2015154787A (ja) 2015-08-27
JP2017029172A (ja) 2017-02-09
US20140363848A1 (en) 2014-12-11
ES2716402T3 (es) 2019-06-12
WO2010062776A3 (en) 2010-08-19
EP2927317B1 (en) 2018-12-19
EP2927317A1 (en) 2015-10-07
JP6150847B2 (ja) 2017-06-21
US9023633B2 (en) 2015-05-05
JP2012507986A (ja) 2012-04-05
WO2010062776A2 (en) 2010-06-03

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