JP2014520814A5 - - Google Patents
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- Publication number
- JP2014520814A5 JP2014520814A5 JP2014519246A JP2014519246A JP2014520814A5 JP 2014520814 A5 JP2014520814 A5 JP 2014520814A5 JP 2014519246 A JP2014519246 A JP 2014519246A JP 2014519246 A JP2014519246 A JP 2014519246A JP 2014520814 A5 JP2014520814 A5 JP 2014520814A5
- Authority
- JP
- Japan
- Prior art keywords
- fusion protein
- sample
- resin
- protein
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 21
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 15
- 239000012539 chromatography resin Substances 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 238000005341 cation exchange Methods 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 3
- 238000010828 elution Methods 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- 239000004475 Arginine Substances 0.000 claims 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 229920002684 Sepharose Polymers 0.000 claims 2
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 claims 2
- 238000005349 anion exchange Methods 0.000 claims 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
- 238000005277 cation exchange chromatography Methods 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- 238000000855 fermentation Methods 0.000 claims 2
- 230000004151 fermentation Effects 0.000 claims 2
- 239000012064 sodium phosphate buffer Substances 0.000 claims 2
- 239000012609 strong anion exchange resin Substances 0.000 claims 2
- 239000012607 strong cation exchange resin Substances 0.000 claims 2
- 102000003298 tumor necrosis factor receptor Human genes 0.000 claims 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 229920002564 Polyethylene Glycol 3500 Polymers 0.000 claims 1
- 239000012614 Q-Sepharose Substances 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000003957 anion exchange resin Substances 0.000 claims 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 239000000919 ceramic Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 102000037865 fusion proteins Human genes 0.000 claims 1
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000012562 protein A resin Substances 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- UYPYRKYUKCHHIB-UHFFFAOYSA-N trimethylamine N-oxide Chemical compound C[N+](C)(C)[O-] UYPYRKYUKCHHIB-UHFFFAOYSA-N 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161505710P | 2011-07-08 | 2011-07-08 | |
| US61/505,710 | 2011-07-08 | ||
| PCT/US2012/045339 WO2013009526A1 (en) | 2011-07-08 | 2012-07-03 | Method for purifying fc-fusion protein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014520814A JP2014520814A (ja) | 2014-08-25 |
| JP2014520814A5 true JP2014520814A5 (enExample) | 2015-07-30 |
| JP6280499B2 JP6280499B2 (ja) | 2018-02-14 |
Family
ID=47506409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014519246A Expired - Fee Related JP6280499B2 (ja) | 2011-07-08 | 2012-07-03 | Ｆｃ融合タンパク質の精製方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9556258B2 (enExample) |
| EP (1) | EP2729482B1 (enExample) |
| JP (1) | JP6280499B2 (enExample) |
| KR (1) | KR20140043934A (enExample) |
| CN (1) | CN103814044A (enExample) |
| AU (2) | AU2012282960A1 (enExample) |
| BR (1) | BR112014000352A2 (enExample) |
| CA (1) | CA2840951A1 (enExample) |
| DK (1) | DK2729482T3 (enExample) |
| WO (1) | WO2013009526A1 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11201401562RA (en) | 2011-10-18 | 2014-09-26 | Coherus Biosciences Inc | Etanercept formulations stabilized with sodium chloride |
| AU2013315750B9 (en) * | 2012-09-11 | 2018-11-15 | Coherus Biosciences, Inc. | Correctly folded etanercept in high purity and excellent yield |
| AU2014240596B2 (en) | 2013-03-14 | 2018-09-27 | Amgen Inc. | Removal of leaked affinity purification ligand |
| SG11201507875RA (en) * | 2013-03-26 | 2015-10-29 | Coherus Biosciences Inc | Protein production method |
| EP3102589A1 (en) * | 2014-02-04 | 2016-12-14 | Biogen MA Inc. | Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications |
| EP3137209B1 (en) | 2014-05-02 | 2022-10-05 | W.R. Grace & CO. - CONN. | Functionalized support material and methods of making and using functionalized support material |
| JP6661547B2 (ja) | 2014-05-13 | 2020-03-11 | アムジエン・インコーポレーテツド | フィルタ及び濾過プロセスで用いるためのプロセス制御システム及び方法 |
| WO2015189832A1 (en) | 2014-06-13 | 2015-12-17 | Lupin Limited | Process for the purification of tnfr:fc fusion protein |
| FR3025515B1 (fr) * | 2014-09-05 | 2016-09-09 | Lab Francais Du Fractionnement | Procede de purification d'un anticorps monoclonal |
| US11053504B2 (en) * | 2014-10-24 | 2021-07-06 | The University Of Chicago | Heat-inducible self-assembling protein domains |
| US20160130324A1 (en) | 2014-10-31 | 2016-05-12 | Shire Human Genetic Therapies, Inc. | C1 Inhibitor Fusion Proteins and Uses Thereof |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US10988527B2 (en) | 2014-12-31 | 2021-04-27 | Lg Chem, Ltd. | Method for preparing TNFR-Fc fusion protein containing target content of impurities |
| SG10201911134QA (en) * | 2015-06-05 | 2020-01-30 | Grace W R & Co | Adsorbent bioprocessing clarification agents and methods of making and using the same |
| AU2016329001A1 (en) | 2015-09-22 | 2018-03-29 | Genentech, Inc. | Expression of Fc-containing proteins |
| US20170101435A1 (en) * | 2015-10-13 | 2017-04-13 | Therapeutic Proteins International, LLC | Harvesting and perfusion apparatus |
| WO2017087882A1 (en) | 2015-11-19 | 2017-05-26 | Shire Human Genetic Therapies, Inc. | Recombinant human c1 esterase inhibitor and uses thereof |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| JP7106187B2 (ja) | 2016-05-11 | 2022-07-26 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを保存する方法 |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| ES2874974T3 (es) | 2016-05-11 | 2021-11-05 | Cytiva Bioprocess R & D Ab | Matriz de separación |
| ES2909833T3 (es) | 2016-05-11 | 2022-05-10 | Cytiva Bioprocess R & D Ab | Método de limpieza y/o desinfección de una matriz de separación |
| KR102435801B1 (ko) * | 2016-07-22 | 2022-08-25 | 암젠 인크 | Fc-함유 단백질의 정제 방법 |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| CN109929038B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | Vegf捕获剂融合蛋白的纯化方法 |
| CN109929027B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | 采用线性洗脱步骤的重组融合蛋白纯化方法 |
| US12233356B2 (en) * | 2019-03-21 | 2025-02-25 | Lonza Sales Ag | Process for preparing extracellular vesicles |
| IL292940A (en) * | 2019-11-22 | 2022-07-01 | Morphosys Ag | Collection of extractant during antibody chromatography |
| AU2021262609A1 (en) | 2020-05-01 | 2022-12-22 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| US11416468B2 (en) * | 2020-07-21 | 2022-08-16 | International Business Machines Corporation | Active-active system index management |
| CN112574321B (zh) * | 2020-12-30 | 2023-10-20 | 上海赛金生物医药有限公司 | 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法 |
| US20240148866A1 (en) * | 2021-02-11 | 2024-05-09 | Denali Therapeutics Inc. | Anti-transferrin receptor fusion proteins and methods of use thereof |
| AU2022358612A1 (en) * | 2021-09-28 | 2024-04-11 | Kashiv Biosciences, Llc | An improved process of purification of fusion protein |
| EP4408879A4 (en) * | 2021-09-28 | 2025-08-27 | Kashiv Biosciences Llc | IMPROVED PROCESS FOR PURIFYING FUSION PROTEIN |
| CN114891061A (zh) * | 2022-04-25 | 2022-08-12 | 杭州艾策生物技术有限公司 | 一种糖基化重组蛋白的纯化方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998031806A2 (en) | 1997-01-21 | 1998-07-23 | Human Genome Sciences, Inc. | Fc RECEPTORS AND POLYPEPTIDES |
| EP1006183A1 (en) | 1998-12-03 | 2000-06-07 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Recombinant soluble Fc receptors |
| PL374311A1 (en) * | 2001-12-21 | 2005-10-03 | Immunex Corporation | Methods for purifying protein |
| WO2004008100A2 (en) * | 2002-07-15 | 2004-01-22 | Immunex Corporation | Methods and media for controlling sialylation of proteins produced by mammalian cells |
| GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| DE602004026343D1 (de) * | 2003-10-24 | 2010-05-12 | Amgen Inc | Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen |
| CA2562766A1 (en) * | 2004-04-14 | 2005-10-27 | F. Hoffmann-La Roche Ag | Purified interleukin-15/fc fusion protein and preparation thereof |
| BRPI0716139A2 (pt) * | 2006-08-28 | 2013-09-17 | Ares Trading Sa | processo para purificaÇço de proteÍnas de fusço fc |
| JP2010501622A (ja) * | 2006-08-28 | 2010-01-21 | アレス トレーディング ソシエテ アノニム | Ｆｃ−融合タンパク質の精製法 |
| WO2008085988A1 (en) * | 2007-01-05 | 2008-07-17 | Amgen Inc. | Methods of purifying proteins |
| ES2391931T3 (es) | 2008-09-15 | 2012-12-03 | Emd Millipore Corporation | Procedimientos para cuantificar la filtración de proteína desde resinas de cromatografía de afinidad basada en proteína |
| WO2010127069A1 (en) | 2009-04-29 | 2010-11-04 | Schering Corporation | Antibody purification |
| ES2817802T3 (es) * | 2009-08-07 | 2021-04-08 | Emd Millipore Corp | Métodos para purificar una proteína objetivo de una o más impurezas en una muestra |
| US9540426B2 (en) * | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
-
2012
- 2012-07-03 JP JP2014519246A patent/JP6280499B2/ja not_active Expired - Fee Related
- 2012-07-03 CN CN201280043885.2A patent/CN103814044A/zh active Pending
- 2012-07-03 CA CA2840951A patent/CA2840951A1/en not_active Abandoned
- 2012-07-03 DK DK12811874.2T patent/DK2729482T3/en active
- 2012-07-03 KR KR1020147002981A patent/KR20140043934A/ko not_active Withdrawn
- 2012-07-03 EP EP12811874.2A patent/EP2729482B1/en not_active Not-in-force
- 2012-07-03 BR BR112014000352A patent/BR112014000352A2/pt not_active IP Right Cessation
- 2012-07-03 US US14/131,280 patent/US9556258B2/en not_active Expired - Fee Related
- 2012-07-03 WO PCT/US2012/045339 patent/WO2013009526A1/en not_active Ceased
- 2012-07-03 AU AU2012282960A patent/AU2012282960A1/en not_active Abandoned
-
2017
- 2017-07-14 AU AU2017204893A patent/AU2017204893A1/en not_active Abandoned
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