JP2014520814A5 - - Google Patents
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- Publication number
- JP2014520814A5 JP2014520814A5 JP2014519246A JP2014519246A JP2014520814A5 JP 2014520814 A5 JP2014520814 A5 JP 2014520814A5 JP 2014519246 A JP2014519246 A JP 2014519246A JP 2014519246 A JP2014519246 A JP 2014519246A JP 2014520814 A5 JP2014520814 A5 JP 2014520814A5
- Authority
- JP
- Japan
- Prior art keywords
- fusion protein
- sample
- resin
- protein
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 21
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 15
- 239000012539 chromatography resin Substances 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 238000005341 cation exchange Methods 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 4
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 3
- 238000010828 elution Methods 0.000 claims 3
- 238000005406 washing Methods 0.000 claims 3
- 239000004475 Arginine Substances 0.000 claims 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 229920002684 Sepharose Polymers 0.000 claims 2
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 claims 2
- 238000005349 anion exchange Methods 0.000 claims 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 2
- 238000005277 cation exchange chromatography Methods 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- 238000000855 fermentation Methods 0.000 claims 2
- 230000004151 fermentation Effects 0.000 claims 2
- 239000012064 sodium phosphate buffer Substances 0.000 claims 2
- 239000012609 strong anion exchange resin Substances 0.000 claims 2
- 239000012607 strong cation exchange resin Substances 0.000 claims 2
- 102000003298 tumor necrosis factor receptor Human genes 0.000 claims 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 229920002564 Polyethylene Glycol 3500 Polymers 0.000 claims 1
- 239000012614 Q-Sepharose Substances 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000003957 anion exchange resin Substances 0.000 claims 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 239000000919 ceramic Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 102000037865 fusion proteins Human genes 0.000 claims 1
- 229960000789 guanidine hydrochloride Drugs 0.000 claims 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000012562 protein A resin Substances 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- UYPYRKYUKCHHIB-UHFFFAOYSA-N trimethylamine N-oxide Chemical compound C[N+](C)(C)[O-] UYPYRKYUKCHHIB-UHFFFAOYSA-N 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161505710P | 2011-07-08 | 2011-07-08 | |
| US61/505,710 | 2011-07-08 | ||
| PCT/US2012/045339 WO2013009526A1 (en) | 2011-07-08 | 2012-07-03 | Method for purifying fc-fusion protein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014520814A JP2014520814A (ja) | 2014-08-25 |
| JP2014520814A5 true JP2014520814A5 (enExample) | 2015-07-30 |
| JP6280499B2 JP6280499B2 (ja) | 2018-02-14 |
Family
ID=47506409
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014519246A Expired - Fee Related JP6280499B2 (ja) | 2011-07-08 | 2012-07-03 | Ｆｃ融合タンパク質の精製方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9556258B2 (enExample) |
| EP (1) | EP2729482B1 (enExample) |
| JP (1) | JP6280499B2 (enExample) |
| KR (1) | KR20140043934A (enExample) |
| CN (1) | CN103814044A (enExample) |
| AU (2) | AU2012282960A1 (enExample) |
| BR (1) | BR112014000352A2 (enExample) |
| CA (1) | CA2840951A1 (enExample) |
| DK (1) | DK2729482T3 (enExample) |
| WO (1) | WO2013009526A1 (enExample) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012326170B2 (en) | 2011-10-18 | 2016-12-22 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with metal ions |
| KR102250937B1 (ko) * | 2012-09-11 | 2021-05-12 | 코히러스 바이오사이언시즈, 인코포레이티드 | 고순도 및 탁월한 수율의 정확하게 폴딩된 에타너셉트 |
| PL3395423T3 (pl) * | 2013-03-14 | 2024-03-18 | Amgen Inc. | Usuwanie wypłukanych ligandów do oczyszczania na zasadzie powinowactwa |
| SG10201802023RA (en) * | 2013-03-26 | 2018-05-30 | Coherus Biosciences Inc | Protein production method |
| NZ722391A (en) * | 2014-02-04 | 2022-12-23 | Biogen Ma Inc | Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications |
| JP6914189B2 (ja) | 2014-05-02 | 2021-08-04 | ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ | 官能化担体材料並びに官能化担体材料を作製及び使用する方法 |
| KR102707370B1 (ko) * | 2014-05-13 | 2024-09-13 | 암겐 인코포레이티드 | 필터 및 여과 공정과 함께 사용하기 위한 공정 제어 시스템 및 방법 |
| WO2015189832A1 (en) | 2014-06-13 | 2015-12-17 | Lupin Limited | Process for the purification of tnfr:fc fusion protein |
| FR3025515B1 (fr) * | 2014-09-05 | 2016-09-09 | Lab Francais Du Fractionnement | Procede de purification d'un anticorps monoclonal |
| WO2016065273A1 (en) * | 2014-10-24 | 2016-04-28 | The University Of Chicago | Heat-inducible self-assembling protein domains |
| US20160130324A1 (en) | 2014-10-31 | 2016-05-12 | Shire Human Genetic Therapies, Inc. | C1 Inhibitor Fusion Proteins and Uses Thereof |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| KR102018608B1 (ko) | 2014-12-31 | 2019-09-06 | 주식회사 엘지화학 | 목적하는 함량으로 불순물을 포함하는 TNFR-Fc 융합 단백질의 제조방법 |
| CN107921407B (zh) * | 2015-06-05 | 2022-07-05 | 格雷斯公司 | 生物处理吸附澄清剂及其制备和使用方法 |
| EP3353288A1 (en) | 2015-09-22 | 2018-08-01 | H. Hoffnabb-La Roche Ag | Expression of fc-containing proteins |
| US20170101435A1 (en) * | 2015-10-13 | 2017-04-13 | Therapeutic Proteins International, LLC | Harvesting and perfusion apparatus |
| CN108463243B (zh) | 2015-11-19 | 2022-06-14 | 夏尔人类遗传性治疗公司 | 重组人c1酯酶抑制剂及其用途 |
| EP3455241B1 (en) | 2016-05-11 | 2022-02-23 | Cytiva BioProcess R&D AB | Method of cleaning and/or sanitizing a separation matrix |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| JP7122974B2 (ja) | 2016-05-11 | 2022-08-22 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 変異免疫グロブリン結合ポリペプチド |
| WO2017194592A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Method of storing a separation matrix |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US11312745B2 (en) * | 2016-07-22 | 2022-04-26 | Amgen Inc. | Methods of purifying Fc-containing proteins |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| ES3064802T3 (en) * | 2016-10-21 | 2026-04-29 | Amgen Inc | Pharmaceutical formulations and methods of making the same |
| CN109929038B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | Vegf捕获剂融合蛋白的纯化方法 |
| CN109929027B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | 采用线性洗脱步骤的重组融合蛋白纯化方法 |
| WO2019246153A1 (en) | 2018-06-19 | 2019-12-26 | Bristol-Myers Squibb Company | Methods of purifying proteins using chromatography |
| EP3941607A1 (en) * | 2019-03-21 | 2022-01-26 | Codiak BioSciences, Inc. | Process for preparing extracellular vesicles |
| US20220411465A1 (en) * | 2019-11-22 | 2022-12-29 | Morphosys Ag | Eluate collection during antibody chromatography |
| AU2021262609A1 (en) | 2020-05-01 | 2022-12-22 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| US11416468B2 (en) * | 2020-07-21 | 2022-08-16 | International Business Machines Corporation | Active-active system index management |
| CN112574321B (zh) * | 2020-12-30 | 2023-10-20 | 上海赛金生物医药有限公司 | 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法 |
| MA71665A (fr) * | 2021-02-11 | 2025-05-30 | Denali Therapeutics Inc. | Protéines de fusion du récepteur anti-transferrine et leurs procédés d'utilisation |
| EP4408857A4 (en) * | 2021-09-28 | 2025-11-05 | Kashiv Biosciences Llc | IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN |
| AU2022354256A1 (en) * | 2021-09-28 | 2024-04-11 | Kashiv Biosciences, Llc | An improved process for purification of fusion protein |
| CN114891061A (zh) * | 2022-04-25 | 2022-08-12 | 杭州艾策生物技术有限公司 | 一种糖基化重组蛋白的纯化方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001509030A (ja) | 1997-01-21 | 2001-07-10 | ヒューマン ジノーム サイエンシーズ，インコーポレイテッド | Ｆｃレセプターおよびポリペプチド |
| EP1006183A1 (en) | 1998-12-03 | 2000-06-07 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Recombinant soluble Fc receptors |
| JP4363986B2 (ja) * | 2001-12-21 | 2009-11-11 | イミュネックス・コーポレーション | タンパク質の精製方法 |
| CA2492267C (en) * | 2002-07-15 | 2013-09-10 | Immunex Corporation | Methods and media for controlling sialylation of proteins produced by mammalian cells |
| GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| US7427659B2 (en) | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| CN1946739A (zh) * | 2004-04-14 | 2007-04-11 | 豪夫迈－罗氏公司 | 纯化的白细胞介素－15/Fc融合蛋白及其制备 |
| JP2010501622A (ja) * | 2006-08-28 | 2010-01-21 | アレス トレーディング ソシエテ アノニム | Ｆｃ−融合タンパク質の精製法 |
| KR20090058532A (ko) | 2006-08-28 | 2009-06-09 | 아레스 트레이딩 에스.에이. | Ｆｃ융합 단백질을 정제하는 방법 |
| WO2008085988A1 (en) | 2007-01-05 | 2008-07-17 | Amgen Inc. | Methods of purifying proteins |
| WO2010030393A1 (en) | 2008-09-15 | 2010-03-18 | Millipore Corporation | Methods for quantifying protein leakage from protein based affinity chromatography resins |
| US20120282654A1 (en) | 2009-04-29 | 2012-11-08 | Schering Corporation | Antibody purification |
| US8536316B2 (en) | 2009-08-07 | 2013-09-17 | Emd Millipore Corporation | Methods for purifying a target protein from one or more impurities in a sample |
| US9540426B2 (en) | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
-
2012
- 2012-07-03 JP JP2014519246A patent/JP6280499B2/ja not_active Expired - Fee Related
- 2012-07-03 KR KR1020147002981A patent/KR20140043934A/ko not_active Withdrawn
- 2012-07-03 DK DK12811874.2T patent/DK2729482T3/en active
- 2012-07-03 WO PCT/US2012/045339 patent/WO2013009526A1/en not_active Ceased
- 2012-07-03 CN CN201280043885.2A patent/CN103814044A/zh active Pending
- 2012-07-03 BR BR112014000352A patent/BR112014000352A2/pt not_active IP Right Cessation
- 2012-07-03 EP EP12811874.2A patent/EP2729482B1/en not_active Not-in-force
- 2012-07-03 US US14/131,280 patent/US9556258B2/en not_active Expired - Fee Related
- 2012-07-03 CA CA2840951A patent/CA2840951A1/en not_active Abandoned
- 2012-07-03 AU AU2012282960A patent/AU2012282960A1/en not_active Abandoned
-
2017
- 2017-07-14 AU AU2017204893A patent/AU2017204893A1/en not_active Abandoned
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