DK2729482T3 - PROCEDURE FOR CLEANING FC-FUSION PROTEIN - Google Patents

PROCEDURE FOR CLEANING FC-FUSION PROTEIN Download PDF

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Publication number
DK2729482T3
DK2729482T3 DK12811874.2T DK12811874T DK2729482T3 DK 2729482 T3 DK2729482 T3 DK 2729482T3 DK 12811874 T DK12811874 T DK 12811874T DK 2729482 T3 DK2729482 T3 DK 2729482T3
Authority
DK
Denmark
Prior art keywords
protein
chromatography
fusion protein
product
resin
Prior art date
Application number
DK12811874.2T
Other languages
Danish (da)
English (en)
Inventor
Ijeoma Cynthia Ikechukwu
Joseph Nti-Gyabaah
Matthew Petroff
Christine Lee
Original Assignee
Merck Sharp & Dohme
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp & Dohme filed Critical Merck Sharp & Dohme
Application granted granted Critical
Publication of DK2729482T3 publication Critical patent/DK2729482T3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
DK12811874.2T 2011-07-08 2012-07-03 PROCEDURE FOR CLEANING FC-FUSION PROTEIN DK2729482T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161505710P 2011-07-08 2011-07-08
PCT/US2012/045339 WO2013009526A1 (en) 2011-07-08 2012-07-03 Method for purifying fc-fusion protein

Publications (1)

Publication Number Publication Date
DK2729482T3 true DK2729482T3 (en) 2018-05-07

Family

ID=47506409

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12811874.2T DK2729482T3 (en) 2011-07-08 2012-07-03 PROCEDURE FOR CLEANING FC-FUSION PROTEIN

Country Status (10)

Country Link
US (1) US9556258B2 (enExample)
EP (1) EP2729482B1 (enExample)
JP (1) JP6280499B2 (enExample)
KR (1) KR20140043934A (enExample)
CN (1) CN103814044A (enExample)
AU (2) AU2012282960A1 (enExample)
BR (1) BR112014000352A2 (enExample)
CA (1) CA2840951A1 (enExample)
DK (1) DK2729482T3 (enExample)
WO (1) WO2013009526A1 (enExample)

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WO2015189832A1 (en) 2014-06-13 2015-12-17 Lupin Limited Process for the purification of tnfr:fc fusion protein
FR3025515B1 (fr) * 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
US11053504B2 (en) * 2014-10-24 2021-07-06 The University Of Chicago Heat-inducible self-assembling protein domains
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10988527B2 (en) 2014-12-31 2021-04-27 Lg Chem, Ltd. Method for preparing TNFR-Fc fusion protein containing target content of impurities
SG10201911134QA (en) * 2015-06-05 2020-01-30 Grace W R & Co Adsorbent bioprocessing clarification agents and methods of making and using the same
AU2016329001A1 (en) 2015-09-22 2018-03-29 Genentech, Inc. Expression of Fc-containing proteins
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
WO2017087882A1 (en) 2015-11-19 2017-05-26 Shire Human Genetic Therapies, Inc. Recombinant human c1 esterase inhibitor and uses thereof
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
JP7106187B2 (ja) 2016-05-11 2022-07-26 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを保存する方法
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
ES2874974T3 (es) 2016-05-11 2021-11-05 Cytiva Bioprocess R & D Ab Matriz de separación
ES2909833T3 (es) 2016-05-11 2022-05-10 Cytiva Bioprocess R & D Ab Método de limpieza y/o desinfección de una matriz de separación
KR102435801B1 (ko) * 2016-07-22 2022-08-25 암젠 인크 Fc-함유 단백질의 정제 방법
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN109929027B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
US12233356B2 (en) * 2019-03-21 2025-02-25 Lonza Sales Ag Process for preparing extracellular vesicles
IL292940A (en) * 2019-11-22 2022-07-01 Morphosys Ag Collection of extractant during antibody chromatography
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
US20240148866A1 (en) * 2021-02-11 2024-05-09 Denali Therapeutics Inc. Anti-transferrin receptor fusion proteins and methods of use thereof
AU2022358612A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process of purification of fusion protein
EP4408879A4 (en) * 2021-09-28 2025-08-27 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFYING FUSION PROTEIN
CN114891061A (zh) * 2022-04-25 2022-08-12 杭州艾策生物技术有限公司 一种糖基化重组蛋白的纯化方法

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Also Published As

Publication number Publication date
AU2012282960A1 (en) 2014-01-16
US20140187751A1 (en) 2014-07-03
EP2729482A1 (en) 2014-05-14
KR20140043934A (ko) 2014-04-11
JP6280499B2 (ja) 2018-02-14
AU2017204893A1 (en) 2017-08-03
CA2840951A1 (en) 2013-01-17
BR112014000352A2 (pt) 2017-02-14
US9556258B2 (en) 2017-01-31
WO2013009526A1 (en) 2013-01-17
EP2729482A4 (en) 2015-02-25
JP2014520814A (ja) 2014-08-25
CN103814044A (zh) 2014-05-21
EP2729482B1 (en) 2018-03-07

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