CA2840951A1 - Methods for purifying fc-fusion protein - Google Patents

Methods for purifying fc-fusion protein Download PDF

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Publication number
CA2840951A1
CA2840951A1 CA2840951A CA2840951A CA2840951A1 CA 2840951 A1 CA2840951 A1 CA 2840951A1 CA 2840951 A CA2840951 A CA 2840951A CA 2840951 A CA2840951 A CA 2840951A CA 2840951 A1 CA2840951 A1 CA 2840951A1
Authority
CA
Canada
Prior art keywords
protein
chromatography
fusion protein
product
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2840951A
Other languages
English (en)
French (fr)
Inventor
Ijeoma Cynthia IKECHUKWU
Joseph Nti-Gyabaah
Matthew PETROFF
Christine Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme LLC
Original Assignee
Merck Sharp and Dohme LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp and Dohme LLC filed Critical Merck Sharp and Dohme LLC
Publication of CA2840951A1 publication Critical patent/CA2840951A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
CA2840951A 2011-07-08 2012-07-03 Methods for purifying fc-fusion protein Abandoned CA2840951A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161505710P 2011-07-08 2011-07-08
US61/505,710 2011-07-08
PCT/US2012/045339 WO2013009526A1 (en) 2011-07-08 2012-07-03 Method for purifying fc-fusion protein

Publications (1)

Publication Number Publication Date
CA2840951A1 true CA2840951A1 (en) 2013-01-17

Family

ID=47506409

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2840951A Abandoned CA2840951A1 (en) 2011-07-08 2012-07-03 Methods for purifying fc-fusion protein

Country Status (10)

Country Link
US (1) US9556258B2 (enExample)
EP (1) EP2729482B1 (enExample)
JP (1) JP6280499B2 (enExample)
KR (1) KR20140043934A (enExample)
CN (1) CN103814044A (enExample)
AU (2) AU2012282960A1 (enExample)
BR (1) BR112014000352A2 (enExample)
CA (1) CA2840951A1 (enExample)
DK (1) DK2729482T3 (enExample)
WO (1) WO2013009526A1 (enExample)

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EP3395423B1 (en) 2013-03-14 2023-09-20 Amgen Inc. Removal of leaked affinity purification ligand
SG11201507875RA (en) * 2013-03-26 2015-10-29 Coherus Biosciences Inc Protein production method
KR102382402B1 (ko) 2014-02-04 2022-04-01 바이오젠 엠에이 인코포레이티드 번역 후 변형을 강화시키기 위한 통류 방식 양이온 교환 크로마토그래피의 용도
US11389783B2 (en) 2014-05-02 2022-07-19 W.R. Grace & Co.-Conn. Functionalized support material and methods of making and using functionalized support material
MX383879B (es) 2014-05-13 2025-03-14 Amgen Inc Sistema y métodos de control del proceso para usar con filtros y procesos de filtración.
BR112016029157A8 (pt) 2014-06-13 2021-07-06 Lupin Ltd processo para purificar a proteína de fusão tnfr:fc
FR3025515B1 (fr) * 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
WO2016065273A1 (en) * 2014-10-24 2016-04-28 The University Of Chicago Heat-inducible self-assembling protein domains
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
KR102018608B1 (ko) * 2014-12-31 2019-09-06 주식회사 엘지화학 목적하는 함량으로 불순물을 포함하는 TNFR-Fc 융합 단백질의 제조방법
PL3302784T3 (pl) * 2015-06-05 2022-01-17 W.R. Grace & Co.-Conn. Adsorbentowe środki klarujące do bioprzetwarzania oraz sposoby ich wytwarzania i stosowania
CN108138147B (zh) 2015-09-22 2022-11-01 豪夫迈·罗氏有限公司 含Fc蛋白的表达
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
WO2017087882A1 (en) 2015-11-19 2017-05-26 Shire Human Genetic Therapies, Inc. Recombinant human c1 esterase inhibitor and uses thereof
US11708390B2 (en) 2016-05-11 2023-07-25 Cytiva Bioprocess R&D Ab Method of storing a separation matrix
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
ES2909833T3 (es) 2016-05-11 2022-05-10 Cytiva Bioprocess R & D Ab Método de limpieza y/o desinfección de una matriz de separación
IL285146B (en) * 2016-07-22 2022-09-01 Amgen Inc Methods for purifying proteins involving fc
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
CN109929027B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
US12233356B2 (en) * 2019-03-21 2025-02-25 Lonza Sales Ag Process for preparing extracellular vesicles
CA3155160A1 (en) * 2019-11-22 2021-05-27 Morphosys Ag Eluate collection during antibody chromatography
US20230167153A1 (en) 2020-05-01 2023-06-01 Kashiv Biosciences, Llc An improved process of purification of protein
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
WO2022174114A1 (en) * 2021-02-11 2022-08-18 Denali Therapeutics Inc. Anti-transferrin receptor fusion proteins and methods of use thereof
EP4408857A4 (en) * 2021-09-28 2025-11-05 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
CA3233422A1 (en) * 2021-09-28 2023-04-06 Kashiv Biosciences, Llc An improved process for purification of fusion protein
CN114891061A (zh) * 2022-04-25 2022-08-12 杭州艾策生物技术有限公司 一种糖基化重组蛋白的纯化方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2278154A1 (en) * 1997-01-21 1998-07-23 Human Genome Sciences, Inc. Fc receptors and polypeptides
EP1006183A1 (en) * 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
WO2003059935A2 (en) 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
PL376381A1 (en) 2002-07-15 2005-12-27 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
ATE462716T1 (de) 2003-10-24 2010-04-15 Amgen Inc Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
EP1756155A2 (en) * 2004-04-14 2007-02-28 F.Hoffmann-La Roche Ag PURIFIED INTERLEUKIN-15/Fc FUSION PROTEIN AND PREPARATION THEREOF
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
BRPI0716139A2 (pt) * 2006-08-28 2013-09-17 Ares Trading Sa processo para purificaÇço de proteÍnas de fusço fc
US20080167450A1 (en) 2007-01-05 2008-07-10 Hai Pan Methods of purifying proteins
EP2335076B1 (en) * 2008-09-15 2012-08-08 EMD Millipore Corporation Methods for quantifying protein leakage from protein based affinity chromatography resins
US20120282654A1 (en) * 2009-04-29 2012-11-08 Schering Corporation Antibody purification
CN102574911B (zh) * 2009-08-07 2017-06-06 Emd密理博公司 从样品的一或多种杂质中纯化靶蛋白的方法
US9540426B2 (en) * 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production

Also Published As

Publication number Publication date
US20140187751A1 (en) 2014-07-03
KR20140043934A (ko) 2014-04-11
US9556258B2 (en) 2017-01-31
WO2013009526A1 (en) 2013-01-17
EP2729482A1 (en) 2014-05-14
CN103814044A (zh) 2014-05-21
JP2014520814A (ja) 2014-08-25
EP2729482B1 (en) 2018-03-07
JP6280499B2 (ja) 2018-02-14
AU2012282960A1 (en) 2014-01-16
EP2729482A4 (en) 2015-02-25
DK2729482T3 (en) 2018-05-07
AU2017204893A1 (en) 2017-08-03
BR112014000352A2 (pt) 2017-02-14

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20170630

FZDE Discontinued

Effective date: 20190703