CN103814044A - 纯化fc-融合蛋白的方法 - Google Patents

纯化fc-融合蛋白的方法 Download PDF

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Publication number
CN103814044A
CN103814044A CN201280043885.2A CN201280043885A CN103814044A CN 103814044 A CN103814044 A CN 103814044A CN 201280043885 A CN201280043885 A CN 201280043885A CN 103814044 A CN103814044 A CN 103814044A
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China
Prior art keywords
protein
product
chromatography
resin
sample
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Pending
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CN201280043885.2A
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English (en)
Chinese (zh)
Inventor
I.C.伊克楚库
J.恩蒂-吉亚巴
M.佩特罗夫
C.李
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Merck Sharp and Dohme LLC
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Schering Corp
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Publication of CN103814044A publication Critical patent/CN103814044A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
CN201280043885.2A 2011-07-08 2012-07-03 纯化fc-融合蛋白的方法 Pending CN103814044A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161505710P 2011-07-08 2011-07-08
US61/505710 2011-07-08
PCT/US2012/045339 WO2013009526A1 (en) 2011-07-08 2012-07-03 Method for purifying fc-fusion protein

Publications (1)

Publication Number Publication Date
CN103814044A true CN103814044A (zh) 2014-05-21

Family

ID=47506409

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280043885.2A Pending CN103814044A (zh) 2011-07-08 2012-07-03 纯化fc-融合蛋白的方法

Country Status (10)

Country Link
US (1) US9556258B2 (enExample)
EP (1) EP2729482B1 (enExample)
JP (1) JP6280499B2 (enExample)
KR (1) KR20140043934A (enExample)
CN (1) CN103814044A (enExample)
AU (2) AU2012282960A1 (enExample)
BR (1) BR112014000352A2 (enExample)
CA (1) CA2840951A1 (enExample)
DK (1) DK2729482T3 (enExample)
WO (1) WO2013009526A1 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106536565A (zh) * 2014-06-13 2017-03-22 鲁宾有限公司 一种用于纯化TNFR‑Fc融合蛋白的方法
CN109563125A (zh) * 2016-07-22 2019-04-02 美国安进公司 含有Fc的蛋白的纯化方法
CN109929027A (zh) * 2017-12-15 2019-06-25 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
CN109929038A (zh) * 2017-12-15 2019-06-25 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN112574321A (zh) * 2020-12-30 2021-03-30 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法

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HK1200718A1 (en) 2011-10-18 2015-08-14 科荣生生物科学公司 Etanercept formulations stabilized with sodium chloride
PE20150996A1 (es) * 2012-09-11 2015-08-01 Coherus Biosciences Inc Etanercept correctamente plegado de alta pureza y excelente rendimiento
LT2969099T (lt) 2013-03-14 2018-09-10 Amgen Inc. Pratekėjusio ligando pašalinimas afininiame gryninime
EP2978498B1 (en) * 2013-03-26 2019-07-03 Coherus Biosciences, Inc. Protein production method
WO2015120056A1 (en) 2014-02-04 2015-08-13 Biogen Ma Inc. Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications
CN107847907A (zh) 2014-05-02 2018-03-27 格雷斯公司 官能化载体材料以及制备和使用官能化载体材料的方法
CN112588116A (zh) 2014-05-13 2021-04-02 美国安进公司 用于过滤器和过滤过程的过程控制系统和方法
FR3025515B1 (fr) * 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
US11053504B2 (en) * 2014-10-24 2021-07-06 The University Of Chicago Heat-inducible self-assembling protein domains
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
BR112017014224A2 (pt) 2014-12-31 2018-03-06 Lg Chem, Ltd. método para a preparação de proteína de fusão fc-tnfr que contém teor alvo de impurezas
US10695744B2 (en) * 2015-06-05 2020-06-30 W. R. Grace & Co.-Conn. Adsorbent biprocessing clarification agents and methods of making and using the same
EP3353288A1 (en) 2015-09-22 2018-08-01 H. Hoffnabb-La Roche Ag Expression of fc-containing proteins
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
BR112018010160A8 (pt) 2015-11-19 2019-02-26 Shire Human Genetic Therapies inibidor da c1 esterase humana recombinante e usos do mesmo
EP3455240B1 (en) 2016-05-11 2025-01-01 Cytiva BioProcess R&D AB Method of storing a separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
CN109311948B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 清洁和/或消毒分离基质的方法
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
US12233356B2 (en) * 2019-03-21 2025-02-25 Lonza Sales Ag Process for preparing extracellular vesicles
JP7725780B2 (ja) * 2019-11-22 2025-08-20 モルフォシス・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 抗体クロマトグラフィー工程中の溶出液収集
JP2023523823A (ja) 2020-05-01 2023-06-07 カシーヴ バイオサイエンシズ，リミテッド・ライアビリティ・カンパニー タンパク質の精製の改善されたプロセス
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
JP2024507118A (ja) * 2021-02-11 2024-02-16 デナリ セラピューティクス インコーポレイテッド 抗トランスフェリン受容体融合タンパク質及びその使用方法
EP4408857A4 (en) * 2021-09-28 2025-11-05 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
EP4408879A4 (en) * 2021-09-28 2025-08-27 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFYING FUSION PROTEIN
CN114891061A (zh) * 2022-04-25 2022-08-12 杭州艾策生物技术有限公司 一种糖基化重组蛋白的纯化方法

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WO2004008100A2 (en) * 2002-07-15 2004-01-22 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
CN101541825A (zh) * 2006-08-28 2009-09-23 阿雷斯贸易股份有限公司 Fc融合蛋白的纯化方法

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EP1006183A1 (en) * 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
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EP1687328B1 (en) * 2003-10-24 2010-03-31 Amgen, Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
MXPA06009496A (es) * 2004-04-14 2007-04-09 Hoffmann La Roche Proteina de fusion interleukina-15/fc purificada y preparacion de la misma.
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
WO2008085988A1 (en) 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
US8338577B2 (en) * 2008-09-15 2012-12-25 Emd Millipore Corporation Methods for quantifying protein leakage from protein based affinity chromatography resins
US20120282654A1 (en) * 2009-04-29 2012-11-08 Schering Corporation Antibody purification
WO2011017514A1 (en) * 2009-08-07 2011-02-10 Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2003059935A2 (en) * 2001-12-21 2003-07-24 Immunex Corporation Methods for purifying protein
WO2004008100A2 (en) * 2002-07-15 2004-01-22 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
CN101541825A (zh) * 2006-08-28 2009-09-23 阿雷斯贸易股份有限公司 Fc融合蛋白的纯化方法

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106536565A (zh) * 2014-06-13 2017-03-22 鲁宾有限公司 一种用于纯化TNFR‑Fc融合蛋白的方法
CN109563125A (zh) * 2016-07-22 2019-04-02 美国安进公司 含有Fc的蛋白的纯化方法
CN115925780A (zh) * 2016-07-22 2023-04-07 美国安进公司 含有Fc的蛋白的纯化方法
CN109929027A (zh) * 2017-12-15 2019-06-25 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
CN109929038A (zh) * 2017-12-15 2019-06-25 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN109929027B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
CN112574321A (zh) * 2020-12-30 2021-03-30 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法

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BR112014000352A2 (pt) 2017-02-14
WO2013009526A1 (en) 2013-01-17
JP6280499B2 (ja) 2018-02-14
CA2840951A1 (en) 2013-01-17
KR20140043934A (ko) 2014-04-11
AU2012282960A1 (en) 2014-01-16
US9556258B2 (en) 2017-01-31
JP2014520814A (ja) 2014-08-25
US20140187751A1 (en) 2014-07-03
DK2729482T3 (en) 2018-05-07
AU2017204893A1 (en) 2017-08-03
EP2729482B1 (en) 2018-03-07
EP2729482A4 (en) 2015-02-25
EP2729482A1 (en) 2014-05-14

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Application publication date: 20140521

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