JP2019521986A5 - - Google Patents
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- JP2019521986A5 JP2019521986A5 JP2018565786A JP2018565786A JP2019521986A5 JP 2019521986 A5 JP2019521986 A5 JP 2019521986A5 JP 2018565786 A JP2018565786 A JP 2018565786A JP 2018565786 A JP2018565786 A JP 2018565786A JP 2019521986 A5 JP2019521986 A5 JP 2019521986A5
- Authority
- JP
- Japan
- Prior art keywords
- chromatography
- antibody
- mixed mode
- anion exchange
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000000034 method Methods 0.000 claims 82
- 238000004587 chromatography analysis Methods 0.000 claims 30
- 239000000203 mixture Substances 0.000 claims 28
- 238000012434 mixed-mode chromatography Methods 0.000 claims 27
- 102000004169 proteins and genes Human genes 0.000 claims 22
- 108090000623 proteins and genes Proteins 0.000 claims 22
- 239000006167 equilibration buffer Substances 0.000 claims 19
- 238000005571 anion exchange chromatography Methods 0.000 claims 17
- 239000013627 low molecular weight specie Substances 0.000 claims 14
- 238000005349 anion exchange Methods 0.000 claims 13
- 239000000427 antigen Substances 0.000 claims 13
- 102000036639 antigens Human genes 0.000 claims 13
- 108091007433 antigens Proteins 0.000 claims 13
- 229920001184 polypeptide Polymers 0.000 claims 13
- 102000004196 processed proteins & peptides Human genes 0.000 claims 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims 13
- 239000013628 high molecular weight specie Substances 0.000 claims 12
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 10
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 10
- 210000004027 cell Anatomy 0.000 claims 9
- 102100034608 Angiopoietin-2 Human genes 0.000 claims 8
- 108010048036 Angiopoietin-2 Proteins 0.000 claims 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims 8
- 239000012535 impurity Substances 0.000 claims 7
- 238000004255 ion exchange chromatography Methods 0.000 claims 7
- 239000012160 loading buffer Substances 0.000 claims 7
- 239000011534 wash buffer Substances 0.000 claims 7
- 238000005277 cation exchange chromatography Methods 0.000 claims 6
- 238000010828 elution Methods 0.000 claims 6
- 239000012149 elution buffer Substances 0.000 claims 6
- 230000002378 acidificating effect Effects 0.000 claims 5
- 238000001042 affinity chromatography Methods 0.000 claims 5
- 238000004519 manufacturing process Methods 0.000 claims 5
- 206010028980 Neoplasm Diseases 0.000 claims 4
- 239000007983 Tris buffer Substances 0.000 claims 4
- 201000011510 cancer Diseases 0.000 claims 4
- 239000000710 homodimer Substances 0.000 claims 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
- 241000588724 Escherichia coli Species 0.000 claims 3
- 150000001412 amines Chemical group 0.000 claims 3
- 208000030533 eye disease Diseases 0.000 claims 3
- 239000000463 material Substances 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 238000000108 ultra-filtration Methods 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 2
- 229920000936 Agarose Polymers 0.000 claims 2
- 229920002873 Polyethylenimine Polymers 0.000 claims 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 101710120037 Toxin CcdB Proteins 0.000 claims 2
- 239000012615 aggregate Substances 0.000 claims 2
- 150000001450 anions Chemical class 0.000 claims 2
- 239000013592 cell lysate Substances 0.000 claims 2
- 239000012501 chromatography medium Substances 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 239000003814 drug Substances 0.000 claims 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 239000003446 ligand Substances 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 239000011159 matrix material Substances 0.000 claims 2
- 238000012986 modification Methods 0.000 claims 2
- 230000004048 modification Effects 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 239000001632 sodium acetate Substances 0.000 claims 2
- 235000017281 sodium acetate Nutrition 0.000 claims 2
- WOUANPHGFPAJCA-UHFFFAOYSA-N 2-[benzyl(methyl)amino]ethanol Chemical compound OCCN(C)CC1=CC=CC=C1 WOUANPHGFPAJCA-UHFFFAOYSA-N 0.000 claims 1
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 102000005431 Molecular Chaperones Human genes 0.000 claims 1
- 108010006519 Molecular Chaperones Proteins 0.000 claims 1
- 208000022873 Ocular disease Diseases 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 239000012534 cell culture medium component Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 238000011210 chromatographic step Methods 0.000 claims 1
- 238000011437 continuous method Methods 0.000 claims 1
- 238000011026 diafiltration Methods 0.000 claims 1
- 230000002209 hydrophobic effect Effects 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 238000002305 strong-anion-exchange chromatography Methods 0.000 claims 1
- 229950000449 vanucizumab Drugs 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 238000003989 weak cation exchange chromatography Methods 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022117433A JP7564843B2 (ja) | 2016-06-17 | 2022-07-22 | 多重特異性抗体の精製 |
| JP2024102664A JP2024156655A (ja) | 2016-06-17 | 2024-06-26 | 多重特異性抗体の精製 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662351908P | 2016-06-17 | 2016-06-17 | |
| US62/351,908 | 2016-06-17 | ||
| PCT/US2017/038007 WO2017218977A2 (en) | 2016-06-17 | 2017-06-16 | Purification of multispecific antibodies |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022117433A Division JP7564843B2 (ja) | 2016-06-17 | 2022-07-22 | 多重特異性抗体の精製 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019521986A JP2019521986A (ja) | 2019-08-08 |
| JP2019521986A5 true JP2019521986A5 (enExample) | 2020-08-06 |
Family
ID=59593150
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018565786A Pending JP2019521986A (ja) | 2016-06-17 | 2017-06-16 | 多重特異性抗体の精製 |
| JP2022117433A Active JP7564843B2 (ja) | 2016-06-17 | 2022-07-22 | 多重特異性抗体の精製 |
| JP2024102664A Pending JP2024156655A (ja) | 2016-06-17 | 2024-06-26 | 多重特異性抗体の精製 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022117433A Active JP7564843B2 (ja) | 2016-06-17 | 2022-07-22 | 多重特異性抗体の精製 |
| JP2024102664A Pending JP2024156655A (ja) | 2016-06-17 | 2024-06-26 | 多重特異性抗体の精製 |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US20190256556A1 (enExample) |
| EP (2) | EP3472177B1 (enExample) |
| JP (3) | JP2019521986A (enExample) |
| KR (3) | KR102826635B1 (enExample) |
| CN (1) | CN109563124A (enExample) |
| AR (1) | AR108800A1 (enExample) |
| AU (2) | AU2017286676B2 (enExample) |
| BR (1) | BR112018075516A2 (enExample) |
| CA (1) | CA3026518A1 (enExample) |
| ES (1) | ES2988363T3 (enExample) |
| HR (1) | HRP20241323T1 (enExample) |
| HU (1) | HUE068564T2 (enExample) |
| IL (1) | IL263690B2 (enExample) |
| MX (2) | MX2018015173A (enExample) |
| PL (1) | PL3472177T3 (enExample) |
| SG (1) | SG11201810777WA (enExample) |
| TW (1) | TWI798179B (enExample) |
| WO (1) | WO2017218977A2 (enExample) |
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| US9840553B2 (en) | 2014-06-28 | 2017-12-12 | Kodiak Sciences Inc. | Dual PDGF/VEGF antagonists |
| WO2017117464A1 (en) | 2015-12-30 | 2017-07-06 | Kodiak Sciences Inc. | Antibodies and conjugates thereof |
| US11964998B2 (en) * | 2017-08-30 | 2024-04-23 | Fresenius Kabi Deutschland Gmbh | Method for purifying anti-IL-6 receptor antibodies |
| CN111491951B (zh) * | 2017-12-22 | 2024-05-24 | 豪夫迈·罗氏有限公司 | 通过疏水相互作用色谱法耗尽轻链错配的抗体变体 |
| EP3746538A1 (en) * | 2018-01-30 | 2020-12-09 | Exothera SA | Protein purification process |
| WO2019149693A1 (en) * | 2018-01-30 | 2019-08-08 | Univercells S.A. | Protein purification process and platform |
| MX2020007527A (es) * | 2018-02-06 | 2020-09-09 | Hoffmann La Roche | Tratamiento de enfermedades oftalmologicas. |
| BR112020017872A2 (pt) | 2018-03-02 | 2020-12-22 | Kodiak Sciences Inc. | Anticorpos de il-6 e construtos de fusão e conjugados dos mesmos |
| US20230182041A1 (en) * | 2018-04-25 | 2023-06-15 | Medimmune, Llc | Purification of antibodies |
| CN111423512B (zh) * | 2019-01-10 | 2024-01-05 | 北京比洋生物技术有限公司 | 阻断血管内皮细胞生长且活化t细胞的多靶向融合蛋白和包含其的药物组合物 |
| TWI871300B (zh) * | 2019-01-28 | 2025-02-01 | 美商安進公司 | 藉由將藥物物質和藥物產品過程整體化的生物製劑製造之連續製造過程 |
| AU2020216361A1 (en) | 2019-01-29 | 2021-08-12 | Takeda Pharmaceutical Company Limited | Parathyroid hormone variants |
| EP3962924A1 (en) | 2019-05-03 | 2022-03-09 | Genentech, Inc. | Methods of reducing the enzymatic hydrolysis activity rate in a composition obtained from a purification platform |
| CN114026108B (zh) * | 2019-06-28 | 2024-09-10 | 夏尔巴生物技术(苏州)有限公司 | 无缝连续流层析方法 |
| WO2021072265A1 (en) | 2019-10-10 | 2021-04-15 | Kodiak Sciences Inc. | Methods of treating an eye disorder |
| AU2020377919A1 (en) * | 2019-11-07 | 2022-06-16 | Amgen Inc. | High salt washes during cation exchange chromatography to remove product-realated impurities |
| AU2021208515A1 (en) * | 2020-01-15 | 2022-08-04 | F. Hoffmann-La Roche Ag | Methods to decrease impurities from recombinant protein manufacturing processes |
| KR102154952B1 (ko) * | 2020-02-21 | 2020-09-10 | 프레스티지바이오로직스 주식회사 | 아달리무맙의 Non-Protein A 정제방법 |
| CN115066258A (zh) * | 2020-02-28 | 2022-09-16 | 上海药明生物技术有限公司 | 双特异性抗体的纯化 |
| EP4118093A4 (en) * | 2020-03-11 | 2024-04-17 | Dr. Reddy's Laboratories Ltd. | METHOD FOR PURIFYING A FC FUSION PROTEIN |
| CN115734969A (zh) * | 2020-04-13 | 2023-03-03 | 上海药明生物技术有限公司 | 双特异性抗体的纯化 |
| CN113563469A (zh) * | 2020-04-28 | 2021-10-29 | 江苏中新医药有限公司 | 高回收率纯化阿达木单抗的方法 |
| US20230167153A1 (en) * | 2020-05-01 | 2023-06-01 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| AU2021265591A1 (en) * | 2020-05-01 | 2022-12-22 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| EP4142794A4 (en) * | 2020-05-01 | 2024-10-30 | Kashiv Biosciences, LLC | IMPROVED PROCESS FOR AFFINITY CHROMATOGRAPHY |
| CN114014906B (zh) * | 2020-06-24 | 2024-01-12 | 夏尔巴生物技术(苏州)有限公司 | 一种利用阳离子交换层析纯化疏水性蛋白的方法 |
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| CN114539416B (zh) * | 2020-11-26 | 2024-11-08 | 盛禾(中国)生物制药有限公司 | 一种双特异性抗体的层析纯化工艺 |
| EP4263597A2 (en) * | 2020-12-18 | 2023-10-25 | Kiniksa Pharmaceuticals, Ltd. | Protein compositions and methods for producing and using the same |
| US20240158436A1 (en) * | 2021-03-09 | 2024-05-16 | Jcr Pharmaceuticals Co., Ltd. | Method for Producing Antibody-Lysosomal Enzyme Fusion Protein |
| TW202241924A (zh) * | 2021-04-23 | 2022-11-01 | 大陸商和鉑醫藥（上海）有限責任公司 | 一種雙特異性抗體的純化方法 |
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2017
- 2017-06-16 EP EP17751870.1A patent/EP3472177B1/en active Active
- 2017-06-16 WO PCT/US2017/038007 patent/WO2017218977A2/en not_active Ceased
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- 2017-06-16 KR KR1020197001176A patent/KR20190039929A/ko not_active Ceased
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- 2017-06-16 EP EP24193902.4A patent/EP4549463A3/en active Pending
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