JP2013505739A5 - - Google Patents
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- JP2013505739A5 JP2013505739A5 JP2012532272A JP2012532272A JP2013505739A5 JP 2013505739 A5 JP2013505739 A5 JP 2013505739A5 JP 2012532272 A JP2012532272 A JP 2012532272A JP 2012532272 A JP2012532272 A JP 2012532272A JP 2013505739 A5 JP2013505739 A5 JP 2013505739A5
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- acid bacteria
- cells
- bacteria cells
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 90
- 235000014655 lactic acid Nutrition 0.000 claims description 45
- 239000004310 lactic acid Substances 0.000 claims description 45
- 241000894006 Bacteria Species 0.000 claims description 40
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims description 28
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims description 27
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims description 27
- 108010084631 acetolactate decarboxylase Proteins 0.000 claims description 26
- 108090000623 proteins and genes Proteins 0.000 claims description 24
- 230000002255 enzymatic effect Effects 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 16
- 238000010353 genetic engineering Methods 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 12
- 230000010354 integration Effects 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 239000003550 marker Substances 0.000 claims description 8
- 239000013612 plasmid Substances 0.000 claims description 8
- 230000010076 replication Effects 0.000 claims description 8
- 238000012239 gene modification Methods 0.000 claims description 7
- 230000005017 genetic modification Effects 0.000 claims description 7
- 235000013617 genetically modified food Nutrition 0.000 claims description 7
- 230000004048 modification Effects 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 108091026890 Coding region Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 108010012306 Tn5 transposase Proteins 0.000 claims description 4
- 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 2
- 230000001131 transforming effect Effects 0.000 claims description 2
- 230000002538 fungal effect Effects 0.000 description 9
- 108010008221 formate C-acetyltransferase Proteins 0.000 description 6
- AMIMRNSIRUDHCM-UHFFFAOYSA-N Isopropylaldehyde Chemical compound CC(C)C=O AMIMRNSIRUDHCM-UHFFFAOYSA-N 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- JTEYKUFKXGDTEU-UHFFFAOYSA-N 2,3-dihydroxy-3-methylbutanoic acid Chemical compound CC(C)(O)C(O)C(O)=O JTEYKUFKXGDTEU-UHFFFAOYSA-N 0.000 description 2
- WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 description 2
- QHKABHOOEWYVLI-UHFFFAOYSA-N 3-methyl-2-oxobutanoic acid Chemical compound CC(C)C(=O)C(O)=O QHKABHOOEWYVLI-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 description 2
- 101100454398 Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) ldh1 gene Proteins 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108010060146 pyruvate formate-lyase activating enzyme Proteins 0.000 description 2
- 101100108562 Escherichia coli (strain K12) aldA gene Proteins 0.000 description 1
- 101100269572 Klebsiella aerogenes budA gene Proteins 0.000 description 1
- 101100020699 Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) ldh2 gene Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 101100054327 Mus musculus Abcd1 gene Proteins 0.000 description 1
- 241000202223 Oenococcus Species 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 101100083037 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) act gene Proteins 0.000 description 1
- 101100322937 Streptococcus thermophilus aldC gene Proteins 0.000 description 1
- 101150049512 ald gene Proteins 0.000 description 1
- 101150019439 aldB gene Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- GFAZHVHNLUBROE-UHFFFAOYSA-N hydroxymethyl propionaldehyde Natural products CCC(=O)CO GFAZHVHNLUBROE-UHFFFAOYSA-N 0.000 description 1
- 108010088076 lactate dehydrogenase 2 Proteins 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 101150104734 ldh gene Proteins 0.000 description 1
- 101150109073 ldhD gene Proteins 0.000 description 1
- 101150049339 pflA gene Proteins 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24671709P | 2009-09-29 | 2009-09-29 | |
| US61/246,717 | 2009-09-29 | ||
| PCT/US2010/050705 WO2011041402A1 (en) | 2009-09-29 | 2010-09-29 | Improved flux to acetolactate-derived products in lactic acid bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013505739A JP2013505739A (ja) | 2013-02-21 |
| JP2013505739A5 true JP2013505739A5 (enExample) | 2013-11-14 |
Family
ID=43093547
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012532272A Pending JP2013505739A (ja) | 2009-09-29 | 2010-09-29 | 乳酸菌におけるアセト乳酸誘導生成物への改善されたフラックス |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9169499B2 (enExample) |
| EP (1) | EP2483400A1 (enExample) |
| JP (1) | JP2013505739A (enExample) |
| CN (1) | CN102712912A (enExample) |
| AU (1) | AU2010300722A1 (enExample) |
| BR (1) | BR112012006941A2 (enExample) |
| CA (1) | CA2776151A1 (enExample) |
| IN (1) | IN2012DN02396A (enExample) |
| WO (1) | WO2011041402A1 (enExample) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8945899B2 (en) | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| JP2011510611A (ja) | 2007-02-09 | 2011-04-07 | ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア | 組換え微生物によるバイオ燃料の生成 |
| BRPI0808600A2 (pt) | 2007-04-18 | 2018-12-26 | Du Pont | "método para a conversão de acetolactato em dihidroxi-isovalerato, método para a produção de isobutanol, célula hospedeira recombinante e método para a identificação e isolameno de uma construção genética" |
| US8188250B2 (en) | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
| WO2010037111A1 (en) | 2008-09-29 | 2010-04-01 | Butamax™ Advanced Biofuels LLC | INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST |
| US9260708B2 (en) * | 2009-09-29 | 2016-02-16 | Butamax Advanced Biofuels Llc | Yeast production host cells |
| CA2785660A1 (en) | 2009-12-29 | 2011-07-07 | Butamax(Tm) Advanced Biofuels Llc | Expression of hexose kinase in recombinant host cells |
| BR112012016042A2 (pt) | 2009-12-29 | 2020-09-15 | Butamax Advanced Biofuels Llc | "celula hospedeira microbiana recombinante, metodo para a produção de isobutanol, método para a produção de 2-butanol e método para a produção de 14-butanol |
| MX357290B (es) | 2010-02-17 | 2018-07-04 | Butamax Tm Advanced Biofuels | Mejoramiento de la actividad de proteínas que requieren centros fe-s. |
| CN105950525A (zh) | 2010-09-07 | 2016-09-21 | 布特马斯先进生物燃料有限责任公司 | 整合编码催化丙酮酸至乙酰乳酸的转化的多肽的多核苷酸 |
| KR20140092759A (ko) | 2011-03-24 | 2014-07-24 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 숙주 세포 및 아이소부탄올의 제조 방법 |
| AU2012249389A1 (en) | 2011-04-29 | 2013-11-14 | Danisco Us Inc. | Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities |
| JP6093764B2 (ja) | 2011-07-28 | 2017-03-08 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | ケトイソ吉草酸デカルボキシラーゼ酵素およびその使用方法 |
| CN104321436A (zh) | 2011-12-30 | 2015-01-28 | 布特马斯先进生物燃料有限责任公司 | 醇类的发酵生产 |
| CN102559736B (zh) * | 2012-02-24 | 2014-01-08 | 常茂生物化学工程股份有限公司 | 提高富马酸产量的基因工程菌株及构建方法及其应用 |
| MX2014011355A (es) | 2012-03-23 | 2014-12-05 | Butamax Advanced Biofuels Llc | Complementacion con acetato de medio para butanologenos. |
| KR20150014953A (ko) | 2012-05-11 | 2015-02-09 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 케톨-산 리덕토아이소머라아제 효소 및 사용 방법 |
| CN102816787B (zh) * | 2012-06-20 | 2015-05-06 | 江南大学 | 利用重组大肠杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶 |
| WO2014025604A2 (en) * | 2012-08-07 | 2014-02-13 | Gevo, Inc. | Microorganisms for improved production of fuels, chemicals, and amino acids |
| AU2013305714A1 (en) | 2012-08-22 | 2015-02-05 | Butamax Advanced Biofuels Llc | Production of fermentation products |
| CN102876704A (zh) * | 2012-09-20 | 2013-01-16 | 江南大学 | 利用重组枯草芽孢杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶 |
| WO2014047421A1 (en) | 2012-09-21 | 2014-03-27 | Butamax(Tm) Advanced Biofuels Llc | Production of renewable hydrocarbon compositions |
| CA2886157A1 (en) | 2012-09-26 | 2014-04-03 | Butamax Advanced Biofuels Llc | Polypeptides with ketol-acid reductoisomerase activity |
| US9273330B2 (en) | 2012-10-03 | 2016-03-01 | Butamax Advanced Biofuels Llc | Butanol tolerance in microorganisms |
| US9650624B2 (en) | 2012-12-28 | 2017-05-16 | Butamax Advanced Biofuels Llc | DHAD variants for butanol production |
| CA2905912A1 (en) | 2013-03-14 | 2014-10-02 | E. I. Du Pont De Nemours And Company | Glycerol 3- phosphate dehydrogenase for butanol production |
| WO2014151190A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Dhad variants and methods of screening |
| WO2014144210A2 (en) | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Competitive growth and/or production advantage for butanologen microorganism |
| US20150122558A1 (en) | 2013-09-09 | 2015-05-07 | Columbia Trailer Co., Inc. | Lifting jack assembly with rotatable hydraulic cylinder for steering and method of operation |
| US10597662B2 (en) * | 2013-11-22 | 2020-03-24 | Jmtc Enzyme Corporation | Transformant and process for production thereof, and process for production of lactic acid |
| WO2016025425A1 (en) | 2014-08-11 | 2016-02-18 | Butamax Advanced Biofuels Llc | Yeast preparations and methods of making the same |
| EP3309247A4 (en) * | 2015-06-12 | 2019-01-09 | Cj Cheiljedang Corporation | MICROORGANISM HAVING IMPROVED LACTIC ACID PRODUCTION AND METHOD FOR PRODUCING LACTIC ACID USING THE SAME |
| CN108368523A (zh) * | 2015-10-09 | 2018-08-03 | 丹麦技术大学 | 在代谢工程化的乳酸菌中高水平生产双乙酰 |
| US11242513B2 (en) | 2016-12-14 | 2022-02-08 | Wageningen Universiteit | Thermostable Cas9 nucleases |
| CN108570438B (zh) * | 2017-03-08 | 2020-06-30 | 中国科学院上海高等研究院 | 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌 |
| CN108486176B (zh) * | 2018-05-18 | 2021-08-03 | 天津科技大学 | 一种产乳酸乙酯的酿酒酵母及其构建方法与应用 |
| IT201800007846A1 (it) * | 2018-08-03 | 2020-02-03 | Università Degli Studi Di Milano - Bicocca | Procedimento per la biosintesi cellulare di acido poli d-lattico e di acido poli l-lattico |
| JP7536022B2 (ja) * | 2019-02-01 | 2024-08-19 | アンスティテュ・ナシオナル・ドゥ・ルシェルシュ・プール・ラグリキュルテュール，ラリマンタシオン・エ・ランヴィロンヌマン | 内臓痛の予防および／または処置のためのラクトコッカス・ラクティス株 |
| CN111893083A (zh) * | 2019-05-05 | 2020-11-06 | 中国科学院上海高等研究院 | 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用 |
| CN114457122B (zh) * | 2020-05-13 | 2023-06-20 | 安徽华恒生物科技股份有限公司 | 生产l-缬氨酸的重组微生物及构建方法、应用 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| AU723153B2 (en) * | 1996-08-22 | 2000-08-17 | Chr. Hansen A/S | Metabolically engineered lactic acid bacteria and their use |
| FR2777905B1 (fr) * | 1998-04-23 | 2002-07-19 | Agronomique Inst Nat Rech | Bacteries surproductrices d'alpha-acetolactate, et procede d'obtention |
| DE19855312A1 (de) | 1998-12-01 | 2000-06-08 | Degussa | Verfahren zur fermentativen Herstellung von D-Pantothensäure unter Verwendung coryneformer Bakterien |
| US20080026123A1 (en) | 2003-05-22 | 2008-01-31 | Hilltop Ranch, Inc. | Apparatus and Process for Almond Pasteurization |
| TWI348262B (en) | 2005-02-10 | 2011-09-01 | Bruno Ferrario | A circuit and method for adaptive frequency compensation for dc-to-dc converter |
| JP5804666B2 (ja) | 2005-04-12 | 2015-11-04 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | バイオマスの処理および利用における別の供給流れの集中 |
| BRPI0619325B8 (pt) * | 2005-10-26 | 2020-11-17 | Butamax Advanced Biofuels Llc | célula hospedeira microbiana recombinante e método para produção de isobutanol |
| US8945899B2 (en) * | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| BRPI0808600A2 (pt) | 2007-04-18 | 2018-12-26 | Du Pont | "método para a conversão de acetolactato em dihidroxi-isovalerato, método para a produção de isobutanol, célula hospedeira recombinante e método para a identificação e isolameno de uma construção genética" |
| US8130756B2 (en) | 2007-07-13 | 2012-03-06 | Hewlett-Packard Development Company, L.P. | Tunnel configuration associated with packet checking in a network |
| EP2677028B1 (en) * | 2007-12-20 | 2016-03-09 | Butamax (TM) Advanced Biofuels LLC | Ketol-acid reductoisomerase using NADH |
| US8188250B2 (en) * | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
| US8828695B2 (en) * | 2008-06-04 | 2014-09-09 | Butamax Advanced Biofuels Llc | Method for producing butanol using two-phase extractive fermentation |
| BRPI0909989A2 (pt) * | 2008-06-05 | 2021-06-22 | Butamax Advanced Biofuels Llc | célula de levedura recombinante e método para a produção de um produto |
| WO2010037105A1 (en) | 2008-09-29 | 2010-04-01 | Butamax™ Advanced Biofuels LLC | Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria |
| EP2337869B1 (en) | 2008-09-29 | 2015-08-19 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
| EP2342330A1 (en) * | 2008-09-29 | 2011-07-13 | Butamax Advanced Biofuels Llc | Enhanced iron-sulfur cluster formation for increased dihydroxy-acid dehydratase activity in lactic acid bacteria |
| CA2737112A1 (en) * | 2008-10-27 | 2010-06-03 | Butamax Advanced Biofuels Llc | Carbon pathway optimized production hosts for the production of isobutanol |
| US20110195505A1 (en) * | 2009-10-08 | 2011-08-11 | Butamax(Tm) Advanced Biofuels Llc | Bacterial strains for butanol production |
| EP2957322B1 (en) | 2014-06-18 | 2019-11-20 | Fotona d.o.o. | Laser treatment head and laser system |
| US10078608B2 (en) | 2016-09-16 | 2018-09-18 | Intel Corporation | USB-C multiple connector support for host and device mode configurations |
-
2010
- 2010-09-29 CA CA2776151A patent/CA2776151A1/en not_active Abandoned
- 2010-09-29 BR BR112012006941A patent/BR112012006941A2/pt not_active IP Right Cessation
- 2010-09-29 WO PCT/US2010/050705 patent/WO2011041402A1/en not_active Ceased
- 2010-09-29 IN IN2396DEN2012 patent/IN2012DN02396A/en unknown
- 2010-09-29 AU AU2010300722A patent/AU2010300722A1/en not_active Abandoned
- 2010-09-29 US US12/893,065 patent/US9169499B2/en active Active
- 2010-09-29 CN CN2010800539407A patent/CN102712912A/zh active Pending
- 2010-09-29 JP JP2012532272A patent/JP2013505739A/ja active Pending
- 2010-09-29 EP EP10760890A patent/EP2483400A1/en not_active Withdrawn
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