CN108368523A - 在代谢工程化的乳酸菌中高水平生产双乙酰 - Google Patents

在代谢工程化的乳酸菌中高水平生产双乙酰 Download PDF

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CN108368523A
CN108368523A CN201680071433.3A CN201680071433A CN108368523A CN 108368523 A CN108368523 A CN 108368523A CN 201680071433 A CN201680071433 A CN 201680071433A CN 108368523 A CN108368523 A CN 108368523A
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克里斯汀·索利姆
彼得·鲁达尔·詹森
刘建民
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Danmarks Tekniskie Universitet
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Abstract

本发明提供了一种在有氧条件下能够生产双乙酰的基因修饰的乳酸菌。此外，本发明提供了一种在含铁卟啉源和选自Fe3+、Fe2+和Cu2+的金属离子的存在下，在有氧条件下使用基因修饰的乳酸菌生产双乙酰的方法。该乳酸菌通过缺失其编码具有乳酸脱氢酶(E.C.1.1.1.27/E.C.1.1.1.28)、α‑乙酰乳酸脱羧酶(E.C 4.1.1.5)、形成水的NADH氧化酶(E.C.1.6.3.4)、磷酸转乙酰酶(E.C.2.3.1.8)活性的多肽的基因组中的那些基因而被基因修饰；并且可选地缺乏或缺失编码具有双乙酰还原酶((R)‑乙偶姻形成；EC：1.1.1.303)、D‑乙偶姻还原酶、丁二醇脱氢酶((R,R)‑丁烷‑2,3‑二醇形成；E.C.1.1.1.4/1.1.1‑)和乙醇脱氢酶(E.C.1.2.1.10)活性的多肽的基因。本发明提供了该基因修饰的乳酸菌用于生产双乙酰和食品的用途。

Description

在代谢工程化的乳酸菌中高水平生产双乙酰
技术领域
本发明提供了一种在有氧条件下能够生产双乙酰的基因修饰的乳酸菌。此外，本发明提供了一种在含铁卟啉源和选自Fe3+、Fe2+和Cu2+的金属离子的存在下，在有氧条件下使用基因修饰的乳酸菌生产双乙酰的方法。通过缺失编码具有乳酸脱氢酶(E.C 1.1.1.27/E.C.1.1.1.28)、α-乙酰乳酸脱羧酶(E.C 4.1.1.5)、磷酸转乙酰酶(E.C.2.3.1.8)和形成水的NADH氧化酶(E.C.1.6.3.4)活性的多肽的基因组中的那些基因，来对乳酸菌进行基因修饰；并且可选地进一步缺乏或缺失编码具有双乙酰还原酶((R)-乙偶姻形成；EC：1.1.1.303)、D-乙偶姻还原酶、丁二醇脱氢酶((R,R)丁烷-2,3-二醇形成；E.C.1.1.1.4/1.1.1.-)和乙醇脱氢酶(E.C.1.2.1.10)活性的多肽的基因。本发明提供了基因修饰的乳酸菌用于生产双乙酰或食品的用途。
背景技术
双乙酰是一种高价值的风味化合物，它有助于许多发酵食物的奶油香气，包括奶酪、黄油和奶油。乳酸乳球菌(Lactococcus lactis)具有导致合成内消旋-2,3-丁二醇的天然代谢途径。该途径从糖酵解开始，使用两个丙酮酸分子，其通过α-乙酰乳酸合成酶(ALS)缩合成α-乙酰乳酸。不稳定的乙酰乳酸通过非酶促氧化脱羧转化成双乙酰。然而，由于大部分乙酰乳酸通量通过α-乙酰乳酸脱羧酶(ALDB)的活性转化为乙偶姻，其又可以转化为内消旋-2,3-丁二醇，因此双乙酰积累极低。乳酸乳球菌产生的主要发酵产物包括乳酸、乙醇和乙酸。
与源自病原体的细菌菌株相比，一些乳酸菌特别是乳球菌属(Lactococcus)或乳杆菌属(Lactobacillus)种类特别适用于生产食品级双乙酰，因为它们具有“通常认定为安全”(GRAS)的状态。另外，它们具有高糖酵解通量，以及良好表征的代谢途径。在正常情况下，乳球菌属或乳杆菌属种类发酵，90％以上的葡萄糖转化为乳酸。尽管野生型乳酸菌中双乙酰水平非常低，但代谢工程策略的应用使得这些细菌适合开发能够生产同质双乙酰(homo-diacetyl)的基因修饰的菌株。需要具有GRAS状态的乳酸菌可以从作为底物的葡萄糖产生双乙酰，并且其中葡萄糖向双乙酰的转化具有高mol/mol产率。
发明内容
根据第一实施方式，本发明提供了一种用于生产双乙酰的基因修饰的乳酸菌，其中所述乳酸菌的基因组缺失或缺少编码具有以下酶活性的多肽的基因：
a)乳酸脱氢酶(E.C.1.1.1.27或E.C.1.1.1.28)
b)α-乙酰乳酸脱羧酶(E.C.4.1.1.5)
c)磷酸转乙酰酶(E.C.2.3.1.8)
d)NADH氧化酶(E.C.1.6.3.4)；和
其中所述微生物缺乏编码具有以下的转基因：
e)具有双乙酰还原酶(E.C.1.1.1.304)活性的多肽和
f)具有L-丁二醇脱氢酶(E.C.1.1.1.76)活性的多肽。
优选地，本发明的基因修饰的乳酸菌的基因组另外缺失或缺少编码具有以下酶活性的多肽的基因：
g)双乙酰还原酶(E.C.1.1.1.303)；h)D-乙偶姻还原酶；i)丁二醇脱氢
酶(E.C.1.1.1.4)和j)乙醇脱氢酶(E.C.1.2.1.10)。
优选地，本发明的基因修饰的乳酸菌属于选自由以下组成的组的属：乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、片球菌属(Pediococcus)、明串珠菌属(Leuconostoc)、链球菌属(Streptococcus)、酒球菌属(Oenococcus)和芽孢杆菌属(Bacillus)。
根据第二实施方式，本发明提供了一种生产双乙酰的方法，包括以下步骤：
a.将根据本发明的任何实施方式所述的基因修饰的乳酸菌引入生长培养基以产生培养物，其中所述生长培养基包括原卟啉IX或含铁卟啉的源，
b.在有氧生长条件下培养(a)的培养物；
c.回收由所述培养物产生的双乙酰，和可选地
d.分离该回收的双乙酰。
优选地，含铁卟啉的源是生长培养基中的氯化血红素或血红素，其中氯化血红素或血红素的浓度为所述生长培养基的0.3-5μg/ml。
在用于生产双乙酰的方法的另一实施方式中，步骤(a)中该生长培养基的组合总Fe2+、Fe3+和Cu2+浓度小于20mM。优选地，该用于生产双乙酰的方法包括附加步骤：步骤(b)中产生的培养物补充有选自由Fe2+、Fe3+和Cu2+组成的组的一种或多种金属离子；并且在步骤(c)之前在有氧条件下孵育该补充的培养物。优选地，培养物补充有金属离子的步骤将该培养基中Fe3+和Cu2+；或者Fe2+、Fe3+和Cu2+的组合终浓度增加到至少5mM。
根据第三实施方式，本发明提供了本发明的基因修饰的乳酸菌用于生产双乙酰的用途。
根据第四实施方式，本发明提供了本发明的基因修饰的乳酸菌用于生产食品的用途；例如乳产品(例如发酵食品)；以及包含基因修饰的乳酸菌的食品。
本发明的说明
附图的说明：
图1.卡通图示出了用于过量生产双乙酰的乳酸菌的代谢途径的修饰。(A)呼吸与糖酵解相结合用于生产双乙酰，如背景中突出显示的。竞争途径被失活，其用虚线表示。缩写：LDH，乳酸脱氢酶(lactate dehydrogenase)；ALS(ILVB)，乙酰乳酸合成酶(acetolactate synthase)；ALD，乙酰乳酸脱羧酶(acetolactate decarboxylase)；ButBA，双乙酰还原酶和丁二醇脱氢酶(butanediol dehydrogenase)；PDHc，丙酮酸脱氢酶复合物(pyruvate dehydrogenase complex)；PFL，丙酮酸甲酸裂解酶(pyruvate formatelyase)；PTA，磷酸转乙酰酶(phosphotransacetylase)；ADHE，乙醇脱氢酶(alcoholdehydrogenase)；ACK，醋酸激酶(acetate kinase)；Nox，NADH氧化酶(NADH oxidase)。
图2.呼吸的激活恢复了本发明的乳酸菌的生长。
(A)卡通图显示乳酸乳球菌(L.lactis)中的电子传递链，从电子供体(NoxAB)、电子转移(甲基萘醌)到电子受体(Cyt bd氧化酶)，其由氯化血红素激活。
(B)通过以氯化血红素浓度范围从0.2μg/ml升至5.0μg/ml的细胞密度(OD600nm)随时间(小时)变化的函数所测量的细胞生长。
(C)在以氯化血红素浓度范围从0.3μg/ml升至5.0μg/ml的乳酸乳球菌培养物中的细胞NAD+水平和NADH/NAD+比率。
实验重复进行，误差线表示标准偏差。
图3.不同初始葡萄糖浓度下乳酸乳球菌菌株CS4616m的生长和双乙酰生产。通过细胞密度(OD600nm)和以mM(条)的双乙酰浓度随乳酸乳球菌菌株CS4616m的时间(小时)变化的函数所测量的细胞生长的图形表示。(A)25mM葡萄糖；(B)50mM葡萄糖；(C)75mM葡萄糖；(D)108mM葡萄糖。
图4.乳酸乳球菌菌株CS4616m的增强的双乙酰生产。通过对于在108mM葡萄糖(A)上生长的乳酸乳球菌菌株CS4616m，在OD600nm处的细胞密度和以mM计的产物形成(条)随时间(小时)变化的函数所测量的细胞生长的图形表示。
当在增加水平的Fe3+(B)、Fe2+(C)和Cu2+(D)的存在下培养乳酸乳球菌菌株CS4616m时，乙酰乳酸形成双乙酰随时间(小时)变化的函数的图形表示。在乳酸乳球菌菌株CS4616m培养期间加入金属催化剂的时间点为9.8小时，这由(A)中的直线表示。实验重复进行，误差线表示标准偏差。
图5.双乙酰对乳酸乳球菌CS4616m生长的抑制作用。(A)当在培养物达到0.6的细胞密度(OD600nm)时加入的双乙酰(0-10mM)存在下，通过细胞密度(OD600nm)测量的乳酸乳杆菌菌株CS4616m的细胞生长的图形表示。(B)通过细胞密度(OD600nm)测量的乳酸乳杆菌菌株CS4616m的细胞生长的图形表示，以及在10mM Fe3+(在生长期开始时添加的)存在下的底物/产物谱(profile)。
图6.卡通图显示乳糖操纵子中所含的基因，其存在于源自工业乳品起始菌株NCD0712的pLP712质粒上。该质粒赋予细胞通过由lacEF基因编码的乳糖特异性磷酸转移酶系统(PTS)吸收乳糖的能力，之后磷酸化乳糖被磷酸-β-半乳糖苷酶(lacG基因)水解成葡萄糖和半乳糖-6-磷酸(gal-6-P)。葡萄糖部分进入糖酵解，而gal-6-P通过塔格糖-6-P途径(lacABCD基因)降解。
缩写词和术语：
gi编号：(genlnfo标识符)是一个独特的整数，其标识一个特定的序列，独立于数据库源，其由NCBI分配给所有加工成Entrez的序列，包括来自DDBJ/EMBL/GenBank的核苷酸序列、来自SWISS-PROT、PIR等等的蛋白质序列。
氨基酸序列同一性：如本文所使用的术语“序列同一性”表示具有基本相等长度的两个氨基酸序列之间的同源性程度的定量测量。待比较的两个序列必须通过插入空位或者可替换地在蛋白质序列末端截短来进行对齐以提供最佳拟合。序列同一性可以计算为((Nref-Ndif)100)/(Nref)，其中Ndif是两个被对齐时的序列中不相同残基的总数，并且其中Nref是其中一个序列中残基的数量。序列同一性可以可替换地通过BLAST程序计算，例如BLASTP程序(Pearson W.R和D.J.Lipman(1988))(www.ncbi.nlm.nih.gov/cgi-bin/BLAST)。在本发明的一种实施方式中，使用具有缺省参数的序列对齐方法ClustalW进行对齐，如由Thompson J.等人，1994所描述的，可获得于http:www2.ebi.ac.uk/clustalw/。
优选地，与其比较多肽相比，多肽中一个或多个氨基酸残基的置换、插入、添加或缺失的数目是有限的，即不多于1、2、3、4、5、6、7、8、9或10个置换，不多于1、2、3、4、5、6、7、8、9或10个插入，不多于1、2、3、4、5、6、7、8、9或10个添加，以及不多于1、2、3、4、5、6、7、8、9或10个缺失。优选地，该置换是保守性氨基酸置换：限于以下组的成员内的交换：1组：甘氨酸、丙氨酸、缬氨酸、亮氨酸、异亮氨酸；2组：丝氨酸、半胱氨酸、硒代半胱氨酸、苏氨酸、甲硫氨酸；3组：脯氨酸；4组：苯丙氨酸、酪氨酸、色氨酸；5组：天冬氨酸、谷氨酸、天冬酰胺、谷氨酰胺。
缺失的基因：来自微生物细胞基因组中的基因缺失导致基因功能丧失(敲除)，因此在基因编码多肽处，该缺失导致编码的多肽的表达的丧失。当编码的多肽是酶时，该基因缺失导致微生物细胞中相应多肽的可检测的酶活性丧失。微生物细胞基因组中的缺失基因的特征在于由于至少一个核苷酸的缺失或置换或添加导致由该基因编码的多肽的表达缺失而导致的功能丧失。
双乙酰还原酶(E.C.1.1.1.304)具有(S)-乙偶姻形成活性，并且能够将双乙酰(DA)转化为L-乙偶姻(L-AC)
L-丁二醇脱氢酶(E.C.1.1.1.76)能够将L-乙偶姻(L-AC)转化成S,S-2,3-丁二醇(L-BD)。
天然基因：微生物细胞基因组中的内源性基因，与宿主微生物同源。
转基因编码具有双乙酰还原酶活性(E.C.1.1.1.304)和L-丁二醇脱氢酶活性(E.C.1.1.1.76)的多肽，赋予细胞将双乙酰(L-AC)转化为S,S-2,3-丁二醇(L-BD)的能力。
乳清和乳清渗透物和残留乳清渗透物：乳清是干酪制造的副产品；并且包含具有高营养价值的乳清蛋白和乳糖。通常通过超滤或渗滤法去除乳清蛋白产生乳清蛋白浓缩物和富含乳糖的乳清渗透物。乳清渗透物的乳糖含量取决于处理条件，通常通过反渗透可以达到数百克每升，诸如200g/L。通常通过离心从乳清或富含乳糖的渗透物中去除脂肪产生无脂组合物(乳清或渗透物)。从乳清渗透物中提取乳糖(通常通过乳糖结晶)后获得残留乳清渗透物(也称为渗透物母液)；并且具有约150g/L的较低乳糖含量。
具体实施方式
一：一种用于生产双乙酰的基因修饰的乳酸菌
内源性基因被缺失以增强从丙酮酸到双乙酰的代谢通量。
本发明的乳酸菌适于在有氧条件下从葡萄糖生产双乙酰。本发明的乳酸菌的特征在于由于导致合成乳酸、乙酸和乙醇的途径中的酶的活性降低而从丙酮酸至双乙酰的代谢通量增加。当细菌在有氧条件下在缺少硫辛酸的规定的生长培养基中培养时，通过乳酸菌产生的乙酸和乙醇减少。当细菌在有氧条件下培养时，这会使形成甲酸和乙酰辅酶A的酶丙酮酸甲酸裂解酶失活，它们是乙酸和乙醇途径的前体。由于酶即丙酮酸脱氢酶的活性需要硫辛酸，使用欠缺硫辛酸的生长培养基(补充有乙酸)会使通过丙酮酸脱氢酶的乙酰辅酶A合成以及乙酸和乙醇的下游生产失活。当本发明的乳酸菌在厌氧条件下在欠缺硫辛酸的基本培养基中生长时，通过向生长培养基中加入乙酸来满足对乙酰辅酶A的需求。
在一种实施方式中，通过缺失一种或多种编码乳酸、乙酸和可选的乙醇途径的酶的基因，降低了本发明的乳酸菌中向乳酸、乙酸和乙醇的代谢通量。
一1内源性乳酸合成途径的缺失：本发明的乳酸菌的特征在于敲除编码具有乳酸脱氢酶活性的多肽的一种或多种内源性天然基因，引起在该菌中的乳酸合成途径的阻断。编码乳酸脱氢酶(E.C.1.1.1.27或E.C.1.1.1.28)的至少一种基因(例如ldh)的缺失提供了在乳酸生产中被减少的本发明的乳酸菌。例如，在本发明的乳酸菌属于给定的属的情况下，缺失的内源性基因是编码在该属中具有乳酸脱氢酶活性的多肽的基因。优选地，具有乳酸脱氢酶活性(E.C.1.1.1.27或E.C.1.1.1.28)的多肽具有与下列序列之一至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的氨基酸序列同一性：乳球菌属(Lactococcus)种类中SEQ ID NO：2(例如乳酸乳球菌(Lactococcus lactis))；乳杆菌属(Lactobacillus)种类中SEQ ID NO：4、6或8(例如嗜酸乳杆菌(Lactobacillus acidophilus))；乳杆菌属种类中SEQ ID NO：10(例如德氏乳杆菌(Lactobacillus delbrueckii))；乳杆菌属种类中SEQ ID NO.12、14或16(例如干酪乳杆菌(Lactobacillus casei))，乳杆菌属种类中SEQ ID NO.18或20(例如植物乳杆菌(Lactobacillus plantarum))；片球菌属(Pediococcus)种类中SEQ ID NO：22(例如戊糖片球菌(Pediococcus pentosaceus))，明串珠菌属(Leuconostoc)种类中SEQ ID NO：24或26(例如肠膜明串珠菌(Leuconostoc mesenteroides))，链球菌属(Streptococcus)种类中SEQ ID NO：28(例如嗜热链球菌(Streptococcus thermophilus))，酒球菌属(Oenococcus)种类中SEQ ID NO：30或32(例如酒类酒球菌(Oenococcus oeni))，以及芽孢杆菌属(Bacillus coagulans)种类中SEQ ID NO：34或36(例如凝结芽孢杆菌(Bacilluscoagulans))。
在一种实施方式中，编码具有乳酸脱氢酶酶活性(E.C 1.1.1.27或E.C.1.1.1.28)的多肽的另外的内源性基因从本发明的乳酸菌中缺失。例如，当本发明的乳酸菌属于乳球菌属时，缺失的基因(ldhX)编码具有与SEQ ID NO:38至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的氨基酸序列同一性的多肽。
在一种实施方式中，编码具有乳酸脱氢酶酶活性(E.C 1.1.1.27或E.C.1.1.1.28)的多肽的另外的内源性基因从本发明的乳酸菌中缺失。例如当本发明的乳酸菌属于乳球菌属时，缺失的基因(ldhB)编码具有与SEQ ID NO:40至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的氨基酸序列同一性的多肽。此外，当本发明的乳酸菌属于乳球菌属时，编码分别具有与SEQ ID NO：2、38和40至少70％的氨基酸序列同一性的多肽的三种基因(ldh、ldhB和ldhX)可以被缺失。
一2内源乙酸合成途径的缺失：在一种实施方式中，本发明的乳酸菌的特征在于敲除编码磷酸转乙酰酶(E.C.2.3.1.8)的内源性天然基因，引起在该菌中的乙酸合成途径的阻断。编码磷酸转乙酰酶的基因(例如pta)的缺失提供了在乙酸生产中被阻断的本发明的乳酸菌。例如，在本发明的乳酸菌属于给定的属的情况下，缺失的内源性基因是编码在该属中具有磷酸转乙酰酶活性(E.C.2.3.1.8)的多肽的基因。优选地，具有磷酸转乙酰酶活性的多肽具有与下列序列之一至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的氨基酸序列同一性：乳球菌属种类中SEQ IDNO：42(例如乳酸乳球菌)；乳杆菌属种类中SEQ ID NO：44、46、48或50(例如嗜酸乳杆菌、德氏乳杆菌、干酪乳杆菌、植物乳杆菌)，片球菌属种类中SEQ ID NO：52(例如戊糖片球菌)，明串珠菌属种类中SEQ ID NO：54(例如肠膜明串珠菌)，链球菌属种类中SEQ ID NO：56(例如嗜热链球菌)，酒球菌属种类中SEQ ID NO：58(例如酒类酒球菌)，以及芽孢杆菌属种类中SEQ ID NO：60(例如凝结芽孢杆菌)。
一3内源乙醇合成途径的缺失：在一种实施方式中，本发明的乳酸菌的特征在于敲除编码乙醇脱氢酶(E.C.1.2.1.10)的内源天然基因，引起在该菌中乙醇合成途径的阻断。编码乙醇脱氢酶的基因的缺失提供了在乙醇生产中被阻断的本发明的乳酸菌。
例如，当本发明的乳酸菌属于给定的属时，缺失的内源性基因(例如adhE)是编码在该属中具有乙醇脱氢酶活性(E.C.1.2.1.10)的多肽的基因。优选地，具有乙醇脱氢酶活性的多肽具有与下列序列之一至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的氨基酸序列同一性：乳球菌属种类中SEQID NO：62(例如乳酸乳球菌)；乳杆菌属种类中SEQ ID NO：64(例如嗜酸乳杆菌)；乳杆菌属种类中SEQ ID NO：66或68(例如干酪乳杆菌)；乳杆菌属种类中SEQ ID NO：70(例如，植物乳杆菌)，明串珠菌属种类中SEQ ID NO：72(例如肠膜明串珠菌)，链球菌属种类中SEQ ID NO：74(例如嗜热链球菌)，酒球菌属种类中SEQ ID NO：76(例如酒类酒球菌)，以及芽孢杆菌属种类中SEQ ID NO：78(例如凝结芽孢杆菌)。
缺失内源性基因以增强双乙酰积累
一4内源性2,3-丁二醇合成途径的缺失
本发明的乳酸菌的特征可以在于编码具有α-乙酰乳酸脱羧酶(E.C4.1.1.5)、双乙酰还原酶(EC：1.1.1.303)、D-乙偶姻还原酶和2,3-丁二醇脱氢酶((R,R)-丁烷-2,3-二醇形成；E.C：1.1.1.4/1.1.1.-)活性的酶的内源性天然基因的额外敲除，由此引起在该菌中的通过D-乙偶姻或双乙酰将α-乙酰乳酸转化成2,3-丁二醇的2,3-丁二醇合成途径的阻断。当本发明的乳酸菌缺乏这些编码天然2,3-丁二醇途径的内源性天然基因时；并且还缺乏编码双乙酰还原酶(E.C.1.1.1.304)和L-丁二醇脱氢酶(E.C.1.1.1.76)的转基因时，该细胞不能产生内消旋2,3-丁二醇或其手性形式。
在本发明的乳酸菌属于给定的属的情况下，其缺少编码一种或多种多肽的一种或多种内源性天然基因，该一种或多种多肽具有α-乙酰乳酸脱羧酶活性(E.C 4.1.1.5)、双乙酰还原酶(EC：1.1.1.303)；D-乙偶姻还原酶、2,3-丁二醇脱氢酶(E.C 1.1.1.4/1.1.1.-)活性或它们的任何组合；不需要缺失相应基因以产生本发明的细菌的步骤。因此，缺少内源性天然基因的本发明的乳酸菌可以是由于不存在本发明的乳酸菌中编码和表达所述酶的基因，或者由于从该菌的基因组中缺失相应的基因，该内源性基因表达具有α-乙酰乳酸脱羧酶(E.C4.1.1.5)、双乙酰还原酶(EC.1.1.1.303)、D-乙偶姻还原酶和2,3-丁二醇脱氢酶((R,R)-丁烷-2,3-二醇形成；EC 1.1.1.4/1.1.1.-)活性的酶。
缺失编码α-乙酰乳酸脱羧酶(E.C 4.1.1.5)的内源性天然基因(例如aldB)提供了在D-乙偶姻生产中被阻断的本发明的乳酸菌。例如，在本发明的乳酸菌属于给定的属的情况下，缺失的内源性基因是编码在该属中具有α-乙酰乳酸脱羧酶活性(E.C 4.1.1.5)的多肽的基因。优选地，具有α-乙酰乳酸脱羧酶活性的多肽具有与下列序列之一至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％氨基酸序列同一性：乳球菌属种类中SEQ ID NO：80(例如乳酸乳球菌)；乳杆菌属种类中SEQ ID NO：82或84(例如干酪乳杆菌、植物乳杆菌)，片球菌属种类中SEQ ID NO：86(例如戊糖片球菌)，明串珠菌属种类中SEQ ID NO：88或90(例如肠膜明串珠菌)，链球菌属种类中SEQ ID NO：92(例如嗜热链球菌)，酒球菌属种类中SEQ ID NO：94(例如酒类酒球菌)，以及芽孢杆菌属种类中SEQ ID NO：96(例如凝结芽孢杆菌)。
编码双乙酰还原酶(EC：1.1.1.303)的内源性天然基因(例如dar)的缺失提供了在D-乙偶姻生产中被阻断的本发明的乳酸菌。例如，当本发明的乳酸菌属于乳球菌属(例如乳酸乳球菌)时，缺失的基因(dar)编码具有与SEQ ID NO:98至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的氨基酸序列同一性的多肽。
编码D-乙偶姻还原酶的内源性天然基因(例如ar)的缺失提供了在D-乙偶姻生产中被阻断的本发明的乳酸菌。例如，在本发明的乳酸菌属于给定的属的情况下，缺失的内源性基因是编码在该属中具有D-乙偶姻还原酶活性的多肽的基因。优选地，具有D-乙偶姻还原酶活性的多肽具有与下列序列之一至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％氨基酸序列同一性：乳球菌属种类中SEQ ID NO：100(例如乳酸乳球菌)；片球菌属种类中SEQ ID NO：102(例如戊糖片球菌)，明串珠菌属种类中SEQ ID NO：104或106(例如肠膜明串珠菌)，酒球菌属种类中SEQ ID NO：108或110(例如酒类酒球菌)，芽孢杆菌属种类中SEQ ID NO：112(例如凝结芽孢杆菌)，乳杆菌属种类中SEQ ID NO：198或200(例如布氏乳杆菌(Lactobacillus buchneri))。
编码2,3-丁二醇脱氢酶活性(E.C 1.1.1.4/1.1.1.-)的基因(例如butAB)的缺失提供了在内消旋-2,3-丁二醇生产中被阻断的本发明的乳酸菌。例如，当本发明的乳酸菌属于乳球菌属(例如乳酸乳球菌)时，缺失的基因(butAB)编码具有与SEQ ID NO:114至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％氨基酸序列同一性的多肽。
缺失内源性基因以保持氧化还原等效物的平衡
一5内源性NADH氧化活性的缺失
本发明的乳酸菌的特征在于敲除编码形成水的NADH氧化酶的一种或多种内源性天然基因，引起NADH氧化的阻断，并维持降低的NADH水平。基因(例如noxE)的缺失提供了在NADH氧化中部分阻断的本发明的乳酸菌。
例如，在本发明的乳酸菌属于给定的属的情况下，缺失的内源性基因是编码在该属中具有形成水的NADH氧化酶活性(E.C.1.6.3.4)的多肽的基因。优选地，具有NADH氧化酶活性活性的多肽具有与下列序列之一至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％氨基酸序列同一性：乳球菌属种类中SEQ ID NO：116(例如乳酸乳球菌)；干酪乳杆菌中SEQ ID NO：118，植物乳杆菌中SEQ IDNO：120、122、124、126和128，链球菌属种类中SEQ ID NO：130(例如嗜热链球菌)，芽孢杆菌属种类中SEQ ID NO：132、134和136(例如凝结芽孢杆菌)。
在本发明的乳酸菌属于给定的属的情况下，其缺少编码具有形成水的NADH氧化酶活性(E.C.1.6.3.4)活性的一种或多种多肽的内源性天然基因；不需要缺失相应的基因以产生本发明的细菌的步骤。因此，本发明的乳酸菌缺少表达具有形成水的NADH氧化酶活性(E.C.1.6.3.4)活性的酶的内源性天然基因，这是由于在本发明的乳酸菌中不存在编码所述酶的基因，或由于从该菌的基因组中缺失相应的基因。
本发明的基因修饰的乳酸菌的特征在于以惊人高的量(实施例3：95mM双乙酰的产品水平)、以非常高的产率(实施例3：双乙酰产率：0.87mol mol-1葡萄糖)和非常高的生产率(实施例3：产生的双乙酰：0.58g/L·h)从碳源(例如葡萄糖)生产双乙酰的能力。
一.6编码乳糖分解代谢途径酶的基因：
本发明的乳酸菌可以进一步包含乳糖同化和分解代谢所需的下列天然基因或转基因：
1)编码共同赋予乳糖特异性磷酸转移酶系统(PTS)活性(EC 2.7.1.69)的第一和第二多肽组分的第一和第二基因，其中磷酸化乳糖被细胞同化。第一多肽组分的氨基酸序列具有与由乳酸乳球菌lacE基因编码的磷酸转移酶系统EIICB组分(SEQ ID NO：210)的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性；以及第二多肽组分的氨基酸序列具有与由乳酸乳球菌lacF基因编码的磷酸转移酶系统EIIA组分(SEQ ID NO：212)的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性；和
2)编码具有磷酸-β-D-半乳糖苷酶活性(EC3.2.1.85)的多肽的基因，其将乳糖-6-磷酸水解成葡萄糖和半乳糖-6-磷酸(gal-6-P)，由此葡萄糖部分然后可以进入糖酵解途径。该多肽的氨基酸序列具有与由乳酸乳球菌lacG基因编码的磷酸-β-D-半乳糖苷酶(SEQID NO：214)的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性。另外，需要下述编码塔格糖-6-P途径中的酶的基因，由此gal-6-P被降解并作为甘油醛-3-磷酸进入糖酵解途径：
3)编码共同赋予半乳糖-6-磷酸异构酶活性(EC 5.3.1.26)的第一和第二多肽亚基的第一和第二基因。第一多肽亚基的氨基酸序列具有与由乳酸乳球菌lacA基因编码的半乳糖-6-磷酸异构酶(SEQ ID NO：202)的第一亚基的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性；以及第二多肽亚基的氨基酸序列具有与由乳酸乳球菌lacB基因编码的半乳糖-6-磷酸异构酶(SEQ ID NO：204)的第二亚基的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性；和
4)编码具有D-塔格糖-6-磷酸激酶活性(EC 2.7.1.114)的多肽的基因。该多肽的氨基酸序列具有与由乳酸乳球菌lacC基因编码的D-塔格糖-6-磷酸激酶(SEQ ID NO：206)的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性；和
5)编码具有塔格糖1,6-二磷酸醛缩酶活性(EC 4.1.2.40)的多肽的基因。该多肽的氨基酸序列具有与由乳酸乳球菌lacD基因编码的塔格糖1,6-二磷酸醛缩酶(SEQ ID NO：208)的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性；和
6)可选地，编码具有乳糖转运调节剂活性的多肽的基因。该多肽的氨基酸序列具有与由乳酸乳球菌lacR基因编码的乳糖转运调节剂(SEQ ID NO：216)的氨基酸序列至少70％、72％、74％、76％、78％、80％、82％、84％、86％、88％、90％、92％、94％、96％、98％、99％或100％的序列同一性。
根据本发明的基因修饰的乳酸菌能够使用乳糖作为唯一的碳源来生产双乙酰，该乳酸菌包括编码和能够表达功能性乳糖途径的基因。当乳清渗透物或残留乳清渗透物用作乳糖的源并且是生长培养基的组分时；那么培养基应补充有复合氨基氮的源(包括可溶性蛋白和/或肽；肽和/或氨基酸)。酵母提取物是复合氨基氮的合适源；以及CSL，前提是它以本文所述的水解形式提供。
二包含生产双乙酰途径的基因修饰的乳酸菌
包含用于合成双乙酰的途径的根据本发明的基因修饰的乳酸菌是选自由乳球菌属、乳杆菌属、片球菌属、明串珠菌属、链球菌属、酒球菌属和芽孢杆菌属组成的组的乳酸菌属的成员。本发明的乳酸菌可以是例如选自由乳酸乳球菌、嗜酸乳杆菌、德氏乳杆菌、干酪乳杆菌、植物乳杆菌、戊糖片球菌、肠膜明串珠菌、嗜热链球菌、酒类酒球菌和凝结芽孢杆菌组成的组的乳酸菌中的种类。优选地，本发明的乳酸菌是乳球菌的种类。
三使用本发明的基因修饰的微生物生产双乙酰的方法
通过将细菌引入含有用于双乙酰生物合成的碳源的培养基中，可以使用本发明的乳酸菌生产双乙酰；为培养物提供原卟啉IX或含铁卟啉(例如氯化血红素和高铁血红素)的源并在有氧条件下培养细菌(例如：以允许细菌细胞生长和繁殖以达到期望的生物质)；然后向培养物中加入一种或多种选自Fe2+、Fe3+和Cu2+的组的金属离子，并在有氧培养条件下进一步孵育培养物；以及最后回收由培养物产生的双乙酰，如实施例所示。
在培养步骤期间，本发明的乳酸菌的细胞生长繁殖，其中有限量的含铁卟啉的供应对于有氧条件下的细胞生长是必不可少的。在优选的实施方式中，通过在将乳酸菌引入培养基之前和之后添加到培养基中而将原卟啉IX或含铁卟啉提供给培养物。原卟啉IX或含铁卟啉可以在培养物生长期间连续或分批添加到培养物中。例如，氯化血红蛋白的加入量优选为在液体培养基中的最终浓度为0.1-5μg/ml。令人惊讶的是，即使细胞在该培养步骤期间在有氧条件下生长，由细胞产生的主要发酵产物是α-乙酰乳酸，而双乙酰的积累是有限的。另外令人惊讶地观察到，在培养步骤期间，细胞的生长和繁殖不受在细胞和培养基中α-乙酰乳酸的积累的抑制。使用本发明的乳酸菌细胞生产双乙酰的方法利用了生长细胞对α-乙酰乳酸的耐受性；因为α-乙酰乳酸(双乙酰的直接前体)的水平可以在该培养步骤期间积累而不损害生长或最终的双乙酰生产。由于双乙酰本身显示出抑制细胞生长(参见实施例3.2)，其又限制了α-乙酰乳酸的生产，本发明的方法在培养步骤期间使用支持细胞生长和α-乙酰乳酸生产但限制α-乙酰乳酸到双乙酰的化学氧化的生长条件。由于以下原因，在培养步骤期间提供的生长培养基中下列金属离子的浓度优选为≤10mM Fe2+(例如≤1mMFe2+；≤0.1mM Fe2+)；≤10mM Fe3+(例如≤5mM Fe3+；≤1mM Fe3+；≤0.1mM Fe 3+；≤0.01mMFe3+；)和≤1mM Cu2+(例如≤0.1mM Cu2+；≤0.01mM Cu2+，≤0.001mM Cu2+)。用于培养本发明的乳酸菌的典型生长培养基包括以下金属离子中的一种或多种：浓度范围为0.005-10mMFe2+、0.005-10mM Fe3+和0-1mM Cu2+；对应于在0.005-20mM范围内的Fe2+、Fe3+和Cu2+的总组合浓度。
当供应有合适的碳源时，本发明的乳酸菌会生长并产生α-乙酰乳酸(以及有限量的双乙酰)，该碳源包括葡萄糖、麦芽糖、半乳糖、果糖、蔗糖、阿拉伯糖、木糖、棉子糖、甘露糖和乳糖。
当本发明的乳酸菌是乳酸乳球菌菌株时，优选的培养温度是30℃；而为属于其他属的本发明的乳酸菌的生长选择合适的温度是在本技术人员的能力范围内的。
在培养步骤之后，(其中本发明的乳酸菌的细胞已经被培养以达到期望的生物质)，然后将细胞进一步孵育。在该培养步骤中，由细胞产生并释放到生长培养基中的α-乙酰乳酸通过化学氧化缓慢地转化成双乙酰。因此生产双乙酰的方法可以包括回收双乙酰之前的孵育步骤。令人惊讶的是，已经观察到，在该孵育步骤期间，α-乙酰乳酸(由细胞产生)化学氧化成双乙酰的速率可以通过以Fe2+、Fe3+和Cu2+的形式添加金属离子而提高。因此，在一种实施方式中，这些一种或多种金属离子的一种或多种组合终浓度为≥5mM。优选地，金属离子为组合终浓度至少为6mM、8mM、10mM、12mM、14mM、16mM、18mM、20mM、22mM、24mM、26mM、28mM和30mM的Fe3+和/或Cu2+；更优选在15mM和30mM之间。
由本发明的乳酸菌产生的双乙酰可以从生长培养基中回收；并且在双乙酰为细胞内产物的情况下，通过使细胞膜透化并结合双乙酰的提取，如实施例所示使用标准提取方法，可以从本发明的微生物的细胞中回收它。
使用本发明的乳酸菌，获得产量为0.87mol mo1-1的葡萄糖和滴定度为78mM的双乙酰。这种生产力大大超过了其他生产双乙酰的微生物的报告。
四用于生产本发明的微生物的方法
在宿主乳酸菌中缺失内源性基因以获得根据本发明的基因修饰的乳酸菌可以通过多种方法实现，例如通过用含有对抗生素具有抗性的基因座的线性DNA片段或通过允许快速表型选择的任何其他基因来转化宿主细胞，通过与待缺失基因任一侧的细胞染色体上紧密间隔区域同源的序列而侧接，与recA基因的直接随后的缺失或失活结合。通过选择由抗生素抗性或其他可检测表型显示的同源序列之间的双重交换事件，可以选择已经有效地缺失整个基因的染色体破坏。recA基因的失活或缺失防止发生染色体外的元件的重组或合并，从而产生细菌菌株，该细菌菌株在不存在特异性污染物的情况下对于针对基因工程化的蛋白质功能活性或产生的筛选是有用的。
为了产生本发明的乳酸菌而缺失基因的可替换的方法使用了非复制型载体pCS1966。该方法涉及PCR-扩增800碱基对(bp)dsDNA分子，对应于位于待缺失基因的上游和下游的DNA区域，并使用限制性内切酶和T4连接酶将扩增的分子插入pCS1966。然后将重组质粒引入乳酸乳球菌中，其中整合引起红霉素抗性。随后，在存在5-氟乳清酸(5FO)的情况下进行计数器选择，其中质粒的切除和丢失引起5FO抗性。用于从乳酸乳球菌中缺失ldhX、ldhB、ldh、pta、adhE、butBA、aldB和noxE的该方法在实施例1中被进一步详细描述。
通过涉及诱变和筛选/选择的更传统的方法，也可以实现宿主乳酸菌中内源性基因的缺失以获得根据本发明的基因修饰的乳酸菌。例如，使用例如N-甲基-N'-硝基-N-亚硝基胍(NTG)或UV辐射诱变后，使用含有2,3,5-三苯基四唑的固体培养基可以筛选出LDH(乳酸脱氢酶)突变体。可替换地，在诱变后，可以使用溴化物和溴酸盐的组合来选择低乳酸生产的菌株，如Han等人2013年所述的。可以在诱变后轻易地获得ALDB(α-乙酰乳酸脱羧酶)突变体，例如使用NTG，或在培养基中含有不平衡浓度的亮氨酸相对于缬氨酸和异亮氨酸的培养基中生长(Goupil等人，1996)。可以在各种浓度的乙醛存在下筛选和选择ADHE(乙醇脱氢酶)突变体。随后可通过在缺少硫辛酸的限定的培养基中富集青霉素来选择通过诱变(例如使用NTG)诱导的磷酸转乙酰酶(E.C.2.3.1.8)突变体。在这些条件下，脂肪酸生物合成所需的乙酰辅酶A只能从乙酸获得。那些能够同化乙酸的细胞会增殖并随后通过青霉素的存在被杀死。然后可以通过筛选幸存者来分离具有失活磷酸转乙酰酶的突变体。基于氧水平敏感性，可以选择通过诱变(例如使用NTG)诱导的NADH氧化酶(E.C.1.6.3.4)突变体。可以在UV或化学诱变后选择双乙酰还原酶(E.C.1.1.1.303)、D-乙偶姻还原酶和丁二醇脱氢酶(E.C.1.1.1.4)突变体。例如，由Zhang等人(2013)描述的用于检测BDH活性的过滤测定法，其中将单个菌落挑取到无菌膜滤器上，然后通过用2,6-二氯酚吲哚酚处理将该滤器染成深蓝色。然后将该滤器漂浮在2,3-丁二醇溶液上，其中只有BDH阳性菌株变白，允许选择BDH缺陷型突变体。
通过结合所提及的不同方法，容易获得非-GMO双乙酰生产者。因此，本发明的基因修饰的乳酸菌的特征在于乳酸菌中内源性基因的缺失或敲除，其中功能性缺失或功能性敲除也可通过诱导的诱变和筛选/选择获得。
五检测双乙酰产生的方法
检测和定量由本发明的微生物产生的双乙酰的方法包括与折射率检测结合的高效液相色谱(HPLC)，以相对于标准来鉴定和定量双乙酰(如Benson等人，1996年所述的)，如实施例中所述的和所示的。
六本发明的基因修饰的微生物在生产食品中的用途
对于发酵食品的典型的“黄油”味道和香气，发酵食品依赖于发酵期间产生的双乙酰；而这种味道的不存在降低了对消费者的吸引力。通常用于制造乳制品的乳球菌属，即使在低温下也能将双乙酰降解为乙偶姻和2,3-丁二醇，从而降低产品质量。本发明的基因修饰的微生物的使用克服了这个问题，因为该微生物在乳制品的生产中的发酵期间产生了期望的双乙酰；同时该产品可以随后安全地储存而没有降低双乙酰含量的风险。因此，本发明涵盖以下二者的生产：使用本发明的基因修饰的微生物的发酵食品；以及包括本发明的基因修饰的微生物的发酵乳制品。发酵食品的实例包括：干酪、酸奶、酸奶油和酸牛奶。
实施例
实施例1用于生产双乙酰的乳酸乳球菌菌株的基因修饰
产生能够从葡萄糖生产双乙酰的乳酸乳球菌菌株并且有效地将通量导向该化合物所需的基因修饰包括所有可替换的产物途径的失活，如下所述的。
1.1宿主菌株和质粒：
无质粒乳酸乳球菌乳脂(cremoris)亚种菌株MG1363(Gasson，1983)或其衍生物用作能够产生双乙酰的菌株的基因构建的亲本菌株。大肠杆菌(E.coli)菌株ABLE-C(大肠杆菌C lac(LacZ-)[Kanr McrA-McrCB-McrF-Mrr-HsdR(rk-mk-)][F'proAB lacIqZ△M15Tn10(Tetr)])(Stratagene)用于克隆目的。质粒pCS1966(Solem等人，2008)用于缺失乳酸乳球菌中的各种基因的目的。
1.2DNA技术：
所有操作均根据Sambrook等人1989年进行。使用的PCR引物可见于表1中。PfuX7聚合酶(2010)用于PCR应用。使用修改有在裂解前用20μg溶菌酶每ml处理细胞2小时的针对大肠杆菌描述的方法，分离来自乳酸乳球菌的染色体DNA。使用电穿孔转化大肠杆菌的细胞。通过在含有1％甘氨酸的GM17培养基(Terzaghi等人，1975年)中的生长使乳酸乳球菌的细胞为电感受态，并且乳酸乳球菌的细胞如由Holo和Nes(1989年)所前述的通过电穿孔被转化。质粒载体pCS1966(Solem等人，2008年)用于缺失乳酸乳球菌中的基因。使用从乳酸乳球菌分离的PCR引物和染色体DNA，通过PCR扩增将待缺失的乳酸乳球菌染色体区域的上游和下游约800个碱基对(bp)区域来制备用于缺失染色体基因的质粒。用于扩增该上游和下游区域的引物在表1中表示为“geneX ups.”和“geneX dwn”。然后用引物表中所示的相应的限制性内切酶消化该扩增的片段和质粒pCS1966，然后将片段插入质粒中。将所得到的质粒单独地转化到亲本菌株中，并且按照Solem C等人(2008年)的描述进行基因缺失。具体地，通过电穿孔将质粒转化到菌株中，并且在补充有葡萄糖和红霉素的GM17琼脂平板(Difeo目录No.218571：来自Becton、Dickinson和Company的M17肉汤)上选择包含整合到染色体中的质粒的菌株。之后，将转化体纯化并铺在补充有5-氟乳清酸的SA葡萄糖平板上，从而选择其中通过同源重组丢失质粒的菌株。通过PCR验证了成功的缺失(Solem等人，2008年)。
表1：引物
1.3从乳酸乳球菌乳脂亚种中缺失基因。
从乳酸乳球菌乳脂亚种亲本菌株中缺失以下基因LdhX、LdhB、Ldh、pta、adhE、butBA、aldB和noxE。使用衍生自pCS1966的基因缺失质粒来使该基因缺失，所述基因缺失质粒名称为：pCS4026(LdhX)、pCS4020(LdhB)、pCS4104(Ldh)、pCS4230(pta)、PCS4273(adhE)、pCS4491(butBA)、pCS4495(aldB)和pCS4256(noxE)，如上所述构建的(实施例1.2)。
该基因从乳酸乳球菌乳脂亚种亲本菌株中的缺失通过使用引物774/777(LdhX)、769/771(LdhB)、788/789(Ldh)、880/881(pta)、929/930(adhE)、977/979(butBA)、1117/1119(a/dB)、887/890(noxE)对相应基因进行PCR扩增来验证。
菌株和质粒
＊表明染色体ldh可能通过与pCS4268重组而恢复为野生型；△3ldh＝△ldh△ldhX△ldhB；SP表示合成启动子。
含有三种乳酸脱氢酶缺失(ldh、ldhB、ldhX)的菌株命名为CS4099或MG1363△31dh。通过另外地缺失磷酸转乙酰基酶基因pta和adhE，CS4363衍生自CS4099。缺失三个ldh基因的菌株具有较差的生长特性(CS4363)；因此为了促进CS4363的生长及其之后的基因修饰，CS4363菌株用带有热敏复制子的质粒(pCS4268)转化以产生菌株CS4311，该热敏复制子携带由合成启动子(SP)表达的乳酸乳球菌ldh。该质粒如下制备：使用引物710/926从乳酸乳球菌扩增SP-1dh片段，用XbaI/XhoI消化并插入用相同酶消化的pG+host8质粒(Maguin等人，1996年)，然后将连接的质粒导入CS4363菌株中以产生CS4311。菌株CS4502通过缺失butBA衍生自菌株CS4311，并且CS4525通过缺失的aldB的缺失衍生自菌株CS4502。CS4554是通过缺失noxE衍生自菌株CS4525，但由于缺失的ldh基因座和pG+host8质粒(pCS4564)上的完整ldh基因之间的重组事件，发现该菌株中ldh已经回复到野生型(ldh)。CS4562衍生自菌株CS4554，缺少pG+host8质粒，但被携带大肠杆菌ldhA的其他pG+host8质粒(pCS4564)所取代。以下列方式构建质粒pCS4564：使用1130/1131从大肠杆菌扩增SP-ldhA，用XhoI/XbaI消化并插入用相同酶消化的pG+host8中。然后将从CS4562中缺失染色体ldh，从而产生CS4615(MG1363△ldh△ldhX△ldhB△pta△adhE△butBA△aldB△noxE pCS4564)；此后，通过在5μg/ml氯化血红素存在下于36℃孵育而使pCS4564丢失以产生CS4616m。
1.4基因工程化的乳酸菌菌株的培养
乳酸乳杆菌在30℃下在震动的250ml锥形瓶中补充有2％(w/v)葡萄糖和不同浓度的氯化血红素的富集M17肉汤(Terzaghi等人，1975年)中有氧生长。当需要时，以下列浓度添加抗生素：红霉素：对于大肠杆菌为200μg/ml，以及对于乳酸乳球菌为5μg/ml；四环素：对于大肠杆菌为8μg/ml，以及对于乳酸乳球菌为5μg/ml；氯霉素：对于大肠杆菌为20μg/ml，以及对于乳酸乳球菌为5μg/ml。
1.5基因工程化的乳酸菌菌株中的NADH和NAD+的测量
在指数生长期中期(OD 600nm＝0.6)收获的乳酸乳球菌细胞培养物在液氮中冷冻并储存于-20℃。根据说明书使用试剂盒NAD+/NADH-GloTM测定法(Promega公司)进行NADH和NAD+的提取和定量。通过假定1g(干重)的细胞对应于1.67ml的细胞内体积以及1OD6oonm的细胞密度对应于0.35g/l干细胞质量来估计细胞内NADH和NAD+浓度。
1.6基因工程化的乳酸菌菌株的发酵产物的测量
使用配备有Aminex HPX-87H柱(Bio-Rad公司，美国赫拉克勒斯)和Shodex Rl-101检测器(Showa Denko K.K.，日本东京)的Ultimate 3000高压液相色谱系统(Dionex公司，美国桑尼维尔)检测和定量葡萄糖、乳酸和乙醇。使用DAD-3000二极管阵列检测器(Dionex公司，美国桑尼维尔)检测和定量丙酮酸。柱温箱温度设定为60℃，流动相为5mM H2SO4，流速为0.5ml/min。基于Westerfeld等人(1945年)的比色法测量乙偶姻和α-乙酰乳酸。如由Benson等人(1996年)所述测定双乙酰浓度。
1.7基因工程化的乳酸菌菌株的特性
来自乳酸乳球菌乳脂亚种菌株的乳酸脱氢酶(ldh、ldhB、ldhX)、磷酸转乙酰酶(pta)和乙醇脱氢酶(adhE)通过缺失它们的基因而失活，导致向双乙酰的代谢通量的有效重定向。然而，该菌株的主要发酵产物是R-乙偶姻，并且另外它只能有氧生长。当菌株中的染色体编码的LDH基因和PTA基因也失活时，这导致特定生长速率的大幅下降并降低了后续分子操作所需的转化效率。用具有表达LDH的热敏复制子的质粒(pCS4268或pCS4564)转化至菌株，该菌株允许在厌氧条件下生长以及允许通过天然aldB基因和天然butBA操纵子的缺失进一步基因工程化用于生产同质双乙酰的菌株(图1)。
在天然aldB基因缺失的菌株中，由乙酰乳酸形成双乙酰取决于非酶促氧化脱羧，其需要有氧生长条件。然而，生产具有基因型(MG1363△3ldh△pta△adhE△butBA△aldB)的用于生产双乙酰的乳酸乳球菌菌株是不可能的。在有氧条件下，由于乳酸乳球菌中的NADH氧化酶活性，大部分NADH被消耗在NAD+再生和O2去除中。乳酸乳球菌中NADH氧化酶活性的主要来源是NoxE(>95％)，其是形成水的NADH氧化酶。可能地，NoxE活性不足以提供乳酸乳球菌菌株生产双乙酰所需的辅助因子平衡和氧化应激抗性。因此，为了产生用于生产双乙酰的乳酸乳球菌菌株，NoxE基因缺失；以及探究了激活呼吸的代替方法。细胞膜中的乳酸乳球菌呼吸链包括电子供体(NoxAB)、电子转移(甲基萘醌)和电子受体(Cyt bd)。在乳酸乳球菌中不合成的氯化血红素是Cyt bd活性的重要辅助因子，可用于激活为双乙酰生产工程化的乳酸乳球菌菌株的呼吸作用。氯化血红素的外部供应将有助于NAD+/NADH平衡、质子易位、高生物质产量和低氧化应激。因此，在有氧条件下，活性乳酸乳球菌菌株CS4616m(MG1363△3ldh△pta△adhE△butBA△aldB△noxE)为生产双乙酰被代谢工程化，其中氯化血红素-激活的呼吸与细胞内的糖酵解偶联(图2A)。
1.8将编码乳糖分解代谢途径的基因引入乳酸乳球菌乳脂亚种菌株CS4616m
本文所述的野生型乳酸乳球菌菌株MG1363及其衍生物是无法利用乳糖作为碳源的无质粒菌株。乳球菌属质粒pLP712(55.395kbp)包括编码整个乳糖分解代谢途径的基因(Wegmann等人，2012年)。基于Andersen(1983年)的方法从乳制品分离物NCD0712中提取乳糖代谢质粒pLP712(55,395bp)；然后转化到乳酸乳球菌菌株CS4616m中以产生菌株CS4616m2。
位于pLP712质粒上的乳糖分解代谢途径基因(图2)如下：
1.编码两种亚基多肽的lacAB基因一起具有半乳糖-6-磷酸异构酶活性(EC5.3.1.26)；其中，第一种亚基多肽具有由乳酸乳球菌lacA基因编码的SEQ ID NO：202的氨基酸序列；以及第二种亚基多肽具有由乳酸乳球菌lacB基因编码的SEQ ID NO：204的氨基酸序列；
2.编码具有D-塔格糖-6-磷酸激酶活性(EC 2.7.1.114)的多肽的lacC基因；其中该多肽具有SEQ ID NO：206的氨基酸序列；
3.编码具有塔格糖1,6-二磷酸醛缩酶活性(EC 4.1.2.40)的多肽的lacD基因；其中所述多肽具有SEQ ID NO：208的氨基酸；
4.编码一起具有乳糖特异性磷酸转移酶系统(PTS)活性(EC 2.7.1.69)的两种多肽组分的lacEF基因；其中第一种多肽组分是具有由乳酸乳球菌lacE基因编码的SEQ IDNO：210的氨基酸序列的磷酸转移酶系统EIICB组分；以及第二种多肽组分是具有由乳酸乳球菌lacF基因编码的SEQ ID NO：212的氨基酸序列的磷酸转移酶系统EIIA组分；和
5.编码具有磷酸-β-D-半乳糖苷酶活性(EC 3.2.1.85)的多肽的lacG基因；其中该多肽具有SEQ ID NO：214的磷酸-β-D-半乳糖苷酶的氨基酸序列；和
6.编码SEQ ID NO：216的乳糖转运调节剂的lacR基因
实施例2.适用于生产双乙酰的基因工程化的乳酸乳球菌菌株的呼吸依赖性生长
基因工程化的乳酸乳球菌菌株CS4616m无法在无氧条件下生长，这意味着它失去了发酵能力。然而，当有氯化血红素存在时，乳酸乳球菌菌株的生长在有氧条件下恢复(图2B)。响应于高于0.2μg/ml的氯化血红素浓度，乳酸乳球菌菌株的具体生长速率和最终生物质密度(OD6oonm)增加，其中使用包含2％葡萄糖的生长培养基测量到0.8h-1的生长速率和OD600nm8.5的细胞密度，氯化血红素水平为1μg/ml或以上。氯化血红素浓度升高(大约10μg/ml或以上)对细胞生长具有显著的负面影响(数据未显示)。
测得的乳酸乳球菌菌株的细胞内NAD+浓度从在0.3μg/ml氯化血红素存在下生长时的0.25mM增加到在1μg/ml氯化血红素存在下的0.47mM，而NADH/NAD+比率从0.07急剧下降到0.03(图2C)。高于1μg/ml(例如5μg/ml)的氯化血红素浓度不会进一步增加培养期间获得的最终细胞生物质，也不会增加NADH/NAD+比率。在乳酸乳球菌呼吸链中(图2A)，在氯化血红素存在下，电子从NADH脱氢酶(NoxAB)、甲基萘醌转移至Cyt bd氧化酶。由氯化血红素诱导的基因工程化的菌株CS4616m的观察到的生长和NADH/NAD+比率与以下理论一致：通过呼吸作用可以补充由糖酵解途径生成的NADH以及合适的氯化血红素激活剂可用于在这种菌株中微调呼吸并恢复细胞生长。
实施例3.基因工程化的乳酸乳球菌菌株的生长和双乙酰生产
3.1在氯化血红素存在下从葡萄糖生产双乙酰
当乳酸乳球菌菌株CS4616m在1μg/ml氯化血红素存在下生长时，响应于生长培养基中葡萄糖的浓度，双乙酰的产量增加(图3A-D)。尽管发酵15小时后以25mM葡萄糖为代价形成5.3mM双乙酰；产生的量分别对应于50、75和108mM葡萄糖的消耗而增加至10、16和25mM双乙酰，。在发酵肉汤中未发现可检测到的乳酸、乙酸、乙醇或甲酸，检测到少量的丙酮酸，葡萄糖的乙酰乳酸、乙偶姻和双乙酰的总产率接近0.9mol mol-1(表2)。
表2：用1μg/ml氯化血红素生长的乳酸乳球菌菌株CS4616m的发酵产物。
发酵15h后检测到的发酵产物(mM)谱。
1：总的乙酰乳酸、双乙酰和乙偶姻的产率。2：双乙酰的产率。
当细胞在补充有氯化血红素的葡萄糖上生长时，乳酸乳球菌菌株CS4616m中的碳通量被有效地重定向至乙酰乳酸形成途径。测得的双乙酰产率约为0.2mol mo1-1，而观察到乙酰乳酸在积累(表2)。检测到少量的乙偶姻，这可能归因于ALDC(乙酰乳酸脱羧酶C)的活性或化学非氧化脱羧。
当在存在1μg/ml氯化血红素的情况下用108mM葡萄糖生长时，乳酸乳球菌菌株CS4616m积累了乙酰乳酸(图4A)。发酵11小时后检测到乙酰乳酸浓度为83.5mM，其然后降至65mM，伴随着14.8小时后共同产生25mM双乙酰。这表明从乙酰乳酸到双乙酰的非酶促氧化转化是限制性步骤。
3.2金属离子增强了在氯化血红素的存在下从葡萄糖生产双乙酰
发现添加金属离子Fe3+、Fe2+和Cu2+显著增加了通过乳酸乳球菌菌株CS4616m的细胞生产双乙酰。当细胞进入固定相时，将不同浓度的这些金属催化剂加入到培养基中。如图4B所示，30mM Fe3+引起生产双乙酰的显著增加；由此在2小时后检测到85mM双乙酰的双乙酰浓度，并且在4小时后达到95mM。基于4小时的数据，生产双乙酰的产率和生产率高达0.87mol mo1-1葡萄糖和0.58g/L·h。Fe2+也增强了双乙酰的生产，但是在更长的时间内，在20mM Fe2+存在下4小时后产生78mM双乙酰(图4C)。Cu2+是加速生产双乙酰的最有效的金属离子(图4D)，在30mM Cu2+存在下1小时后产生78mM双乙酰并且生产率达到0.619g/L·h。因此，对于增强双乙酰生产，金属离子Fe3+和Cu2+比Fe2+更有效。
观察到的通过乳酸乳球菌菌株CS4616m的细胞的双乙酰高产率是通过在细胞达到期望的细胞生物质后向细胞添加金属离子来实现的。观察到在乳酸乳球菌菌株CS4616m细胞培养物生长期添加金属离子以抑制其生长。因此Cu2+完全消除细胞生长；而Fe3+和Cu2+(10或20mM)当存在于生长培养基时从生长期开始时抑制生长。在10mM Fe3+存在下生长的培养物的最终生物质仅为3.3(OD600nm)(图5B)，与在不存在Fe3+补充物的情况下的8.5(OD600nm)相比(图5A)。此外，还发现双乙酰本身也抑制乳酸乳球菌菌株CS4616m细胞培养物的细胞生长(图5A)。总之，对于最佳的双乙酰生产，乳酸乳球菌菌株CS4616m应在碳源(例如葡萄糖)存在的有氧条件下培养，在氯化血红素的存在下，直至达到足够的生物质，然后加入金属离子，优选Fe3+或Cu2+，并维持有氧条件，以使积累的乙酰乳酸高产率地转化为双乙酰。响应于Fe3+、Fe2+和Cu2+，观察到的通过乳酸乳球菌菌株CS4616m的细胞生产双乙酰的增加与以下发现一致：这些金属离子充当从乙酰乳酸到双乙酰的非酶促氧化脱羧通量的促进剂。可选地，一旦在原卟啉IX或含铁卟啉(例如氯化血红素)的源的存在下培养之后获得了基因修饰的乳酸乳球菌的足够的生物质，则可通过升高培养物或培养基的温度(例如至60-65摄氏度)来加速积累的乙酰乳酸的转化。
实施例4通过本发明的基因修饰的乳酸乳球菌菌株从乳糖生产双乙酰的乳糖培养基的开发
废物流残留乳清渗透物(RWP)是从乳清渗透物中提取乳糖后获得的渗透母液。测定由阿尔拉食品配料集团(Arla Foods Ingredients Group)P/S(http:// www.arlafoodsingredients.com)提供的RWP的组成并示于表4中。如实施例1.8中所述的测定RWP的过滤的样品的糖组分，并通过以下步骤来测定氨基酸组成：用6M HCl水解过滤的样品、使用邻苯二甲醛衍生氨基酸和使用离子交换色谱分析衍生物，如Barkholt等人(1989年)所述的。
表4残留乳清渗透物a的组成
a残留乳清渗透物是从乳清渗透物提取乳糖后残留的残留物的浓缩物。
鉴于RWP的氨基酸含量相对较低，应向RWP添加氮源以支持生长和双乙酰生产。
玉米浆(CSL)是复合氮的廉价来源。CSL购自Sigma-Aldrich(圣路易斯，MO)，固体含量为40-60％。RWP培养基补充有未处理的CSL或酸处理的CSL以增强CSL的氨基氮含量的可用性。CSL样品经受不同程度的酸水解。使用非常少量的硫酸(0.05-0.5％浓H2SO4加入至具有20-30％w/v固体含量的CSL)进行酸水解。应用以下水解条件来生产玉米浆水解产物(CSLH)。H1条件：将原始CSL用水稀释2倍，然后将50μl浓硫酸与100ml稀释的CSL混合。将混合物在121℃保持15分钟，随后加入10M NaOH溶液将pH调节至6.8-7.1。
CSL的游离氨基酸组成的分析表明，与未处理的玉米浆相比，玉米浆的水解使CSL的游离氨基酸含量增加了约2倍。
表5水解之前和之后的CSL的游离氨基酸组成
实施例5.使用本发明的基因工程化的乳酸乳球菌菌株从乳糖生产双乙酰的方法
在具有补充有不同氮源的1μg/ml氯化血红素的稀释的残留乳清渗透物(RWP)培养基中，使用CS4616m2(MG1363△3ldh△pta△adhE△butBA△aldB△noxE pLP712)进行发酵。如表3中所示，稀释的RWP和2％(w/v)酵母提取物的组合物激发最高的双乙酰产量(57mM或4.9g/L)。水解的玉米浆也是用于支持细胞生长和生产双乙酰的良好氮源(水解条件H1)。
表3来自残留乳清渗透介质的生产双乙酰
a 20小时后向培养基中加入30mM Fe3+，此时培养物进入稳定期。
b水解条件：将原始CSL(约50％(w/v)固体含量)用水稀释2倍，然后将50μl浓硫酸与100ml稀释的CSL混合。将混合物在121℃保持15分钟，随后加入10M NaOH溶液将pH调节至6.8-7.1。
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序列表
<110> 丹麦技术大学（Danish Technical University ）
<120> 在代谢工程化的乳酸菌中高水平生产双乙酰
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Met Arg Lys Val Gly Ile Ile Gly Met Gly His Val Gly Ala Thr Val
1 5 10 15
gca tat act ttg ttt act cat gga att gct gac gaa tta gtt tta att 96
Ala Tyr Thr Leu Phe Thr His Gly Ile Ala Asp Glu Leu Val Leu Ile
20 25 30
gat aaa aat gaa gat aaa gtt gct gct gaa tat aat gac tta cgt gat 144
Asp Lys Asn Glu Asp Lys Val Ala Ala Glu Tyr Asn Asp Leu Arg Asp
35 40 45
tct tta tca aga aat aat tac tac gtt cgt gta act atg caa gat tgg 192
Ser Leu Ser Arg Asn Asn Tyr Tyr Val Arg Val Thr Met Gln Asp Trp
50 55 60
cat gaa tta aaa gat gcg gat att att gta act gct ttt ggt gat att 240
His Glu Leu Lys Asp Ala Asp Ile Ile Val Thr Ala Phe Gly Asp Ile
65 70 75 80
gcg gct agt gtc aag act ggc gat cgt ttt ggt gaa ttt gaa ctt aac 288
Ala Ala Ser Val Lys Thr Gly Asp Arg Phe Gly Glu Phe Glu Leu Asn
85 90 95
gct aag aat gca aaa gaa gtc ggt gaa aag att aag aac act ggt ttt 336
Ala Lys Asn Ala Lys Glu Val Gly Glu Lys Ile Lys Asn Thr Gly Phe
100 105 110
aag ggt gta ctt ctt aat atc tct aat cca tgt gat gct gtt gca caa 384
Lys Gly Val Leu Leu Asn Ile Ser Asn Pro Cys Asp Ala Val Ala Gln
115 120 125
atc ttg caa gaa act act gga tta agt aag aac caa gtt tta ggt act 432
Ile Leu Gln Glu Thr Thr Gly Leu Ser Lys Asn Gln Val Leu Gly Thr
130 135 140
ggt act ttc ttg gat act gca aga atg caa aga att att ggt gaa aag 480
Gly Thr Phe Leu Asp Thr Ala Arg Met Gln Arg Ile Ile Gly Glu Lys
145 150 155 160
cta ggt caa gat cca aaa aat gtt gaa ggt tgg gtt ttg ggt gaa cat 528
Leu Gly Gln Asp Pro Lys Asn Val Glu Gly Trp Val Leu Gly Glu His
165 170 175
ggt tca tca caa ttt atc gct tgg tcc act gtt cgt gtt aac aac aag 576
Gly Ser Ser Gln Phe Ile Ala Trp Ser Thr Val Arg Val Asn Asn Lys
180 185 190
att gct att caa tta ttt agc gaa aat gaa caa aga aag tta aat aag 624
Ile Ala Ile Gln Leu Phe Ser Glu Asn Glu Gln Arg Lys Leu Asn Lys
195 200 205
gta cca aac aag aat tca ttt gtg gtt gca aat gga aaa ggc tac act 672
Val Pro Asn Lys Asn Ser Phe Val Val Ala Asn Gly Lys Gly Tyr Thr
210 215 220
agt tac gca att gct aca tgt gct gta agg ctt att caa gca atc ttt 720
Ser Tyr Ala Ile Ala Thr Cys Ala Val Arg Leu Ile Gln Ala Ile Phe
225 230 235 240
tct gat gct cat tta tat gct cct gta tca gta tac aat cca gaa tac 768
Ser Asp Ala His Leu Tyr Ala Pro Val Ser Val Tyr Asn Pro Glu Tyr
245 250 255
aag act tat att ggc tat cct gca att att ggt cgt gat gga att gaa 816
Lys Thr Tyr Ile Gly Tyr Pro Ala Ile Ile Gly Arg Asp Gly Ile Glu
260 265 270
caa gtg att gaa ttg aaa ctt act tca aat gaa cgt gaa aag ctt caa 864
Gln Val Ile Glu Leu Lys Leu Thr Ser Asn Glu Arg Glu Lys Leu Gln
275 280 285
gca gca gct gat aag atc aag gaa cac ttg gaa caa ctg aag aac aag 912
Ala Ala Ala Asp Lys Ile Lys Glu His Leu Glu Gln Leu Lys Asn Lys
290 295 300
taa 915
<210> 6
<211> 304
<212> PRT
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<400> 6
Met Arg Lys Val Gly Ile Ile Gly Met Gly His Val Gly Ala Thr Val
1 5 10 15
Ala Tyr Thr Leu Phe Thr His Gly Ile Ala Asp Glu Leu Val Leu Ile
20 25 30
Asp Lys Asn Glu Asp Lys Val Ala Ala Glu Tyr Asn Asp Leu Arg Asp
35 40 45
Ser Leu Ser Arg Asn Asn Tyr Tyr Val Arg Val Thr Met Gln Asp Trp
50 55 60
His Glu Leu Lys Asp Ala Asp Ile Ile Val Thr Ala Phe Gly Asp Ile
65 70 75 80
Ala Ala Ser Val Lys Thr Gly Asp Arg Phe Gly Glu Phe Glu Leu Asn
85 90 95
Ala Lys Asn Ala Lys Glu Val Gly Glu Lys Ile Lys Asn Thr Gly Phe
100 105 110
Lys Gly Val Leu Leu Asn Ile Ser Asn Pro Cys Asp Ala Val Ala Gln
115 120 125
Ile Leu Gln Glu Thr Thr Gly Leu Ser Lys Asn Gln Val Leu Gly Thr
130 135 140
Gly Thr Phe Leu Asp Thr Ala Arg Met Gln Arg Ile Ile Gly Glu Lys
145 150 155 160
Leu Gly Gln Asp Pro Lys Asn Val Glu Gly Trp Val Leu Gly Glu His
165 170 175
Gly Ser Ser Gln Phe Ile Ala Trp Ser Thr Val Arg Val Asn Asn Lys
180 185 190
Ile Ala Ile Gln Leu Phe Ser Glu Asn Glu Gln Arg Lys Leu Asn Lys
195 200 205
Val Pro Asn Lys Asn Ser Phe Val Val Ala Asn Gly Lys Gly Tyr Thr
210 215 220
Ser Tyr Ala Ile Ala Thr Cys Ala Val Arg Leu Ile Gln Ala Ile Phe
225 230 235 240
Ser Asp Ala His Leu Tyr Ala Pro Val Ser Val Tyr Asn Pro Glu Tyr
245 250 255
Lys Thr Tyr Ile Gly Tyr Pro Ala Ile Ile Gly Arg Asp Gly Ile Glu
260 265 270
Gln Val Ile Glu Leu Lys Leu Thr Ser Asn Glu Arg Glu Lys Leu Gln
275 280 285
Ala Ala Ala Asp Lys Ile Lys Glu His Leu Glu Gln Leu Lys Asn Lys
290 295 300
<210> 7
<211> 927
<212> DNA
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<220>
<221> CDS
<222> (1)..(927)
<223> 嗜酸乳杆菌-NCFM LBA0910 基因编码乳酸脱氢酶
<400> 7
atg agt aga aaa gtg ttt ctt gta ggt gat ggt gct gtt ggt tca act 48
Met Ser Arg Lys Val Phe Leu Val Gly Asp Gly Ala Val Gly Ser Thr
1 5 10 15
ttt gca aat gac tta ttg caa aat aca act gtt gat gaa tta gcg att 96
Phe Ala Asn Asp Leu Leu Gln Asn Thr Thr Val Asp Glu Leu Ala Ile
20 25 30
ttt gat gtt gct aaa gat cgt cca gtt ggt gat tca atg gat ttg gaa 144
Phe Asp Val Ala Lys Asp Arg Pro Val Gly Asp Ser Met Asp Leu Glu
35 40 45
gat att act cca ttt aca ggt caa act aat att cat cca gca gaa tat 192
Asp Ile Thr Pro Phe Thr Gly Gln Thr Asn Ile His Pro Ala Glu Tyr
50 55 60
agt gat gct aaa gat gca gat gtg tgt gta att act gct ggt gtt cct 240
Ser Asp Ala Lys Asp Ala Asp Val Cys Val Ile Thr Ala Gly Val Pro
65 70 75 80
cgt aaa cct ggt gaa act aga ctt gac tta gtt aat aag aat gta aag 288
Arg Lys Pro Gly Glu Thr Arg Leu Asp Leu Val Asn Lys Asn Val Lys
85 90 95
att tta aag act att gtt gat ccg gtt gtt gaa tcc ggt ttt aag ggt 336
Ile Leu Lys Thr Ile Val Asp Pro Val Val Glu Ser Gly Phe Lys Gly
100 105 110
gta ttt gtt gtt tca gct aac ccg gtt gat att tta acc aca ttg act 384
Val Phe Val Val Ser Ala Asn Pro Val Asp Ile Leu Thr Thr Leu Thr
115 120 125
caa aaa ata tcc ggt ttt cca aaa gat cgt gta att ggt act ggt act 432
Gln Lys Ile Ser Gly Phe Pro Lys Asp Arg Val Ile Gly Thr Gly Thr
130 135 140
tca ctt gat tca atg cgt ctt cgc gtt gaa ttg gca aag aaa ctt aat 480
Ser Leu Asp Ser Met Arg Leu Arg Val Glu Leu Ala Lys Lys Leu Asn
145 150 155 160
gtt cca gta gct aag gtt aac tca atg gtt ctt ggt gaa cac ggt gat 528
Val Pro Val Ala Lys Val Asn Ser Met Val Leu Gly Glu His Gly Asp
165 170 175
act agt ttt gaa aac ttt gac gaa tca act gtt gac aat aag cca ctt 576
Thr Ser Phe Glu Asn Phe Asp Glu Ser Thr Val Asp Asn Lys Pro Leu
180 185 190
cgc gat tac tca gaa atc aat gat aat gtt tta agt gaa att gag tca 624
Arg Asp Tyr Ser Glu Ile Asn Asp Asn Val Leu Ser Glu Ile Glu Ser
195 200 205
gac gtc cgt aaa aag ggt gga aag atc atc act aac aaa gga gct aca 672
Asp Val Arg Lys Lys Gly Gly Lys Ile Ile Thr Asn Lys Gly Ala Thr
210 215 220
ttc tat ggt gtt gct atg atg ctt act caa att gtt agt gct att tta 720
Phe Tyr Gly Val Ala Met Met Leu Thr Gln Ile Val Ser Ala Ile Leu
225 230 235 240
gat aat cgt tca att tgt ttg cca tta tca gcc cca att aat ggt gaa 768
Asp Asn Arg Ser Ile Cys Leu Pro Leu Ser Ala Pro Ile Asn Gly Glu
245 250 255
tat ggc att aag cat gat ctt tac tta ggt act cca act ata att aac 816
Tyr Gly Ile Lys His Asp Leu Tyr Leu Gly Thr Pro Thr Ile Ile Asn
260 265 270
ggt aat ggt att gaa aaa gtt att gaa act aaa ctt tca gat gta gaa 864
Gly Asn Gly Ile Glu Lys Val Ile Glu Thr Lys Leu Ser Asp Val Glu
275 280 285
aaa gct aag atg atc aat tct gca gat aag atg caa gaa gtt tta tca 912
Lys Ala Lys Met Ile Asn Ser Ala Asp Lys Met Gln Glu Val Leu Ser
290 295 300
ggt gtt gaa atg taa 927
Gly Val Glu Met
305
<210> 8
<211> 308
<212> PRT
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<400> 8
Met Ser Arg Lys Val Phe Leu Val Gly Asp Gly Ala Val Gly Ser Thr
1 5 10 15
Phe Ala Asn Asp Leu Leu Gln Asn Thr Thr Val Asp Glu Leu Ala Ile
20 25 30
Phe Asp Val Ala Lys Asp Arg Pro Val Gly Asp Ser Met Asp Leu Glu
35 40 45
Asp Ile Thr Pro Phe Thr Gly Gln Thr Asn Ile His Pro Ala Glu Tyr
50 55 60
Ser Asp Ala Lys Asp Ala Asp Val Cys Val Ile Thr Ala Gly Val Pro
65 70 75 80
Arg Lys Pro Gly Glu Thr Arg Leu Asp Leu Val Asn Lys Asn Val Lys
85 90 95
Ile Leu Lys Thr Ile Val Asp Pro Val Val Glu Ser Gly Phe Lys Gly
100 105 110
Val Phe Val Val Ser Ala Asn Pro Val Asp Ile Leu Thr Thr Leu Thr
115 120 125
Gln Lys Ile Ser Gly Phe Pro Lys Asp Arg Val Ile Gly Thr Gly Thr
130 135 140
Ser Leu Asp Ser Met Arg Leu Arg Val Glu Leu Ala Lys Lys Leu Asn
145 150 155 160
Val Pro Val Ala Lys Val Asn Ser Met Val Leu Gly Glu His Gly Asp
165 170 175
Thr Ser Phe Glu Asn Phe Asp Glu Ser Thr Val Asp Asn Lys Pro Leu
180 185 190
Arg Asp Tyr Ser Glu Ile Asn Asp Asn Val Leu Ser Glu Ile Glu Ser
195 200 205
Asp Val Arg Lys Lys Gly Gly Lys Ile Ile Thr Asn Lys Gly Ala Thr
210 215 220
Phe Tyr Gly Val Ala Met Met Leu Thr Gln Ile Val Ser Ala Ile Leu
225 230 235 240
Asp Asn Arg Ser Ile Cys Leu Pro Leu Ser Ala Pro Ile Asn Gly Glu
245 250 255
Tyr Gly Ile Lys His Asp Leu Tyr Leu Gly Thr Pro Thr Ile Ile Asn
260 265 270
Gly Asn Gly Ile Glu Lys Val Ile Glu Thr Lys Leu Ser Asp Val Glu
275 280 285
Lys Ala Lys Met Ile Asn Ser Ala Asp Lys Met Gln Glu Val Leu Ser
290 295 300
Gly Val Glu Met
305
<210> 9
<211> 924
<212> DNA
<213> 德氏乳杆菌（Lactobacillus delbrueckii）
<220>
<221> CDS
<222> (1)..(924)
<223> 德氏乳杆菌保加利亚亚种 ATCC 11842_ Ldb0120
基因编码乳酸脱氢酶
<400> 9
atg agt aga aaa gtc ctg ctg gtt ggc gac ggt gct gtc ggt tcc aac 48
Met Ser Arg Lys Val Leu Leu Val Gly Asp Gly Ala Val Gly Ser Asn
1 5 10 15
ttt gcc aac gat tta ttg caa aca acg cgg gtg gat gaa ctg gtc atc 96
Phe Ala Asn Asp Leu Leu Gln Thr Thr Arg Val Asp Glu Leu Val Ile
20 25 30
tgc gac ttg aat aaa gac cgg gcc gcg ggc gac tgc ctg gac ctg gaa 144
Cys Asp Leu Asn Lys Asp Arg Ala Ala Gly Asp Cys Leu Asp Leu Glu
35 40 45
gac atg acc tac ttt acg ggc cag acc aag ctg aga gct ggt gac tac 192
Asp Met Thr Tyr Phe Thr Gly Gln Thr Lys Leu Arg Ala Gly Asp Tyr
50 55 60
agc gac gcg gct gac gcc gac gtg gta gtt atc acg gcc ggc gtg ccc 240
Ser Asp Ala Ala Asp Ala Asp Val Val Val Ile Thr Ala Gly Val Pro
65 70 75 80
cgc aag cca ggt gaa agc cgg ctt gac ctg atc aag aag aat gaa gcc 288
Arg Lys Pro Gly Glu Ser Arg Leu Asp Leu Ile Lys Lys Asn Glu Ala
85 90 95
atc ttg cgg agc atc gtt gat cca gtc gta gct tcc ggc ttc agc ggc 336
Ile Leu Arg Ser Ile Val Asp Pro Val Val Ala Ser Gly Phe Ser Gly
100 105 110
atc ttc gtc gtt tcc gcc aac ccg gtt gac atc ttg acc act ttg acc 384
Ile Phe Val Val Ser Ala Asn Pro Val Asp Ile Leu Thr Thr Leu Thr
115 120 125
cag aag ctg tcc ggc ttc cca aag aag cgg gtc atc ggg acc ggg act 432
Gln Lys Leu Ser Gly Phe Pro Lys Lys Arg Val Ile Gly Thr Gly Thr
130 135 140
tcc ctg gac tca gct agt ttg cgg gta gaa ctg gcc aag cgg ctg caa 480
Ser Leu Asp Ser Ala Ser Leu Arg Val Glu Leu Ala Lys Arg Leu Gln
145 150 155 160
gtg cca att gaa tca gtc aac gcc tgg gtt ctg ggt gaa cat ggc gac 528
Val Pro Ile Glu Ser Val Asn Ala Trp Val Leu Gly Glu His Gly Asp
165 170 175
agc agc ttt gag aac ttc tca agt gcc gtg gtc aac ggc aag ccc ttg 576
Ser Ser Phe Glu Asn Phe Ser Ser Ala Val Val Asn Gly Lys Pro Leu
180 185 190
ctt gac tat ccg ggc atg aca gaa gca gcc tta gat gag att gaa gcc 624
Leu Asp Tyr Pro Gly Met Thr Glu Ala Ala Leu Asp Glu Ile Glu Ala
195 200 205
cac gtc cgg gaa aag ggc agc gag att atc gtc aaa aag ggc gca acc 672
His Val Arg Glu Lys Gly Ser Glu Ile Ile Val Lys Lys Gly Ala Thr
210 215 220
tac tat ggc gtg gcc atg atg ctg gcc aag atc gtg acg gcc att ttg 720
Tyr Tyr Gly Val Ala Met Met Leu Ala Lys Ile Val Thr Ala Ile Leu
225 230 235 240
gaa aac aac gac ctg gcc ctg cct ctt tcc gca cca ttg cac ggg gaa 768
Glu Asn Asn Asp Leu Ala Leu Pro Leu Ser Ala Pro Leu His Gly Glu
245 250 255
tac ggg atc aag gat gag atc tac ctg ggc acc ctg gcc atc atc aac 816
Tyr Gly Ile Lys Asp Glu Ile Tyr Leu Gly Thr Leu Ala Ile Ile Asn
260 265 270
gga caa ggg atc agc cac gta ctg gaa ttg ccg tta aat gac agc gaa 864
Gly Gln Gly Ile Ser His Val Leu Glu Leu Pro Leu Asn Asp Ser Glu
275 280 285
ttg gcc aag atg cgg gcc tca gcc gcg acg ata aag gca acc ctg gac 912
Leu Ala Lys Met Arg Ala Ser Ala Ala Thr Ile Lys Ala Thr Leu Asp
290 295 300
tct tta gga taa 924
Ser Leu Gly
305
<210> 10
<211> 307
<212> PRT
<213> 德氏乳杆菌（Lactobacillus delbrueckii）
<400> 10
Met Ser Arg Lys Val Leu Leu Val Gly Asp Gly Ala Val Gly Ser Asn
1 5 10 15
Phe Ala Asn Asp Leu Leu Gln Thr Thr Arg Val Asp Glu Leu Val Ile
20 25 30
Cys Asp Leu Asn Lys Asp Arg Ala Ala Gly Asp Cys Leu Asp Leu Glu
35 40 45
Asp Met Thr Tyr Phe Thr Gly Gln Thr Lys Leu Arg Ala Gly Asp Tyr
50 55 60
Ser Asp Ala Ala Asp Ala Asp Val Val Val Ile Thr Ala Gly Val Pro
65 70 75 80
Arg Lys Pro Gly Glu Ser Arg Leu Asp Leu Ile Lys Lys Asn Glu Ala
85 90 95
Ile Leu Arg Ser Ile Val Asp Pro Val Val Ala Ser Gly Phe Ser Gly
100 105 110
Ile Phe Val Val Ser Ala Asn Pro Val Asp Ile Leu Thr Thr Leu Thr
115 120 125
Gln Lys Leu Ser Gly Phe Pro Lys Lys Arg Val Ile Gly Thr Gly Thr
130 135 140
Ser Leu Asp Ser Ala Ser Leu Arg Val Glu Leu Ala Lys Arg Leu Gln
145 150 155 160
Val Pro Ile Glu Ser Val Asn Ala Trp Val Leu Gly Glu His Gly Asp
165 170 175
Ser Ser Phe Glu Asn Phe Ser Ser Ala Val Val Asn Gly Lys Pro Leu
180 185 190
Leu Asp Tyr Pro Gly Met Thr Glu Ala Ala Leu Asp Glu Ile Glu Ala
195 200 205
His Val Arg Glu Lys Gly Ser Glu Ile Ile Val Lys Lys Gly Ala Thr
210 215 220
Tyr Tyr Gly Val Ala Met Met Leu Ala Lys Ile Val Thr Ala Ile Leu
225 230 235 240
Glu Asn Asn Asp Leu Ala Leu Pro Leu Ser Ala Pro Leu His Gly Glu
245 250 255
Tyr Gly Ile Lys Asp Glu Ile Tyr Leu Gly Thr Leu Ala Ile Ile Asn
260 265 270
Gly Gln Gly Ile Ser His Val Leu Glu Leu Pro Leu Asn Asp Ser Glu
275 280 285
Leu Ala Lys Met Arg Ala Ser Ala Ala Thr Ile Lys Ala Thr Leu Asp
290 295 300
Ser Leu Gly
305
<210> 11
<211> 939
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(939)
<223> 干酪乳杆菌 ATCC 334, LSEI_0634 基因编码乳酸脱氢酶
<400> 11
atg cgg aac aac ggc aat att att tta att ggt gat ggc gcg att gga 48
Met Arg Asn Asn Gly Asn Ile Ile Leu Ile Gly Asp Gly Ala Ile Gly
1 5 10 15
tca agt tat gcg ttc aac tgt ctc acc acc ggc gtc ggc cag agt ctt 96
Ser Ser Tyr Ala Phe Asn Cys Leu Thr Thr Gly Val Gly Gln Ser Leu
20 25 30
ggt att att gat gtt aac gaa aaa cgc gtg caa ggt gac gtc gaa gat 144
Gly Ile Ile Asp Val Asn Glu Lys Arg Val Gln Gly Asp Val Glu Asp
35 40 45
ctt tct gat gcc cta cct tac acg tca cag aag aat att tat gcg gcc 192
Leu Ser Asp Ala Leu Pro Tyr Thr Ser Gln Lys Asn Ile Tyr Ala Ala
50 55 60
agc tat gaa gat tgc aaa tat gcc gat att atc gtc atc aca gcc gga 240
Ser Tyr Glu Asp Cys Lys Tyr Ala Asp Ile Ile Val Ile Thr Ala Gly
65 70 75 80
att gcc caa aag cca ggt cag aca cgc cta caa ctt ttg gcc atc aac 288
Ile Ala Gln Lys Pro Gly Gln Thr Arg Leu Gln Leu Leu Ala Ile Asn
85 90 95
gca aag atc atg aaa gaa atc aca cat aac att atg gca agc ggg ttc 336
Ala Lys Ile Met Lys Glu Ile Thr His Asn Ile Met Ala Ser Gly Phe
100 105 110
aat ggg ttt att tta gtc gca tcc aat cca gtc gat gtc ctt gcc gaa 384
Asn Gly Phe Ile Leu Val Ala Ser Asn Pro Val Asp Val Leu Ala Glu
115 120 125
tta gtc ttg caa gaa tcc ggc ttg cca cgt aat cag gtg ctg gga tca 432
Leu Val Leu Gln Glu Ser Gly Leu Pro Arg Asn Gln Val Leu Gly Ser
130 135 140
ggg acg gcg ctt gat tca gct cgc tta cgg tct gag atc ggt ctg cgt 480
Gly Thr Ala Leu Asp Ser Ala Arg Leu Arg Ser Glu Ile Gly Leu Arg
145 150 155 160
tat aac gtg gac gcc cgg att gtg cac ggc tac atc atg ggt gaa cac 528
Tyr Asn Val Asp Ala Arg Ile Val His Gly Tyr Ile Met Gly Glu His
165 170 175
ggc gat tct gaa ttt cca gtt tgg gac tac acc aac att ggc ggc aaa 576
Gly Asp Ser Glu Phe Pro Val Trp Asp Tyr Thr Asn Ile Gly Gly Lys
180 185 190
ccg att ctt gat tgg att cct aaa gat cgc cag gat aaa gat ctg cct 624
Pro Ile Leu Asp Trp Ile Pro Lys Asp Arg Gln Asp Lys Asp Leu Pro
195 200 205
gat att agc gag cgc gtc aaa acg gct gct tat ggc atc att gag aaa 672
Asp Ile Ser Glu Arg Val Lys Thr Ala Ala Tyr Gly Ile Ile Glu Lys
210 215 220
aaa ggt gcc act ttc tac ggc att gct gcc tca tta acc cgt tta acc 720
Lys Gly Ala Thr Phe Tyr Gly Ile Ala Ala Ser Leu Thr Arg Leu Thr
225 230 235 240
agc gcc ttc ttg aat gat gat cgc gca gca ttc gca atg tcc gtc cat 768
Ser Ala Phe Leu Asn Asp Asp Arg Ala Ala Phe Ala Met Ser Val His
245 250 255
ttg gaa ggc gaa tac ggc ttg tca ggt gtt tcc atc ggt gta ccg gta 816
Leu Glu Gly Glu Tyr Gly Leu Ser Gly Val Ser Ile Gly Val Pro Val
260 265 270
atc ctc ggc gct aat ggc tta gaa cgc atc att gag ctg gat ttg aac 864
Ile Leu Gly Ala Asn Gly Leu Glu Arg Ile Ile Glu Leu Asp Leu Asn
275 280 285
cca gaa gat cat aag cgg tta gcc gat tct gca gct att ttg aaa gaa 912
Pro Glu Asp His Lys Arg Leu Ala Asp Ser Ala Ala Ile Leu Lys Glu
290 295 300
aat ctg aag aag gct caa gaa gct taa 939
Asn Leu Lys Lys Ala Gln Glu Ala
305 310
<210> 12
<211> 312
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 12
Met Arg Asn Asn Gly Asn Ile Ile Leu Ile Gly Asp Gly Ala Ile Gly
1 5 10 15
Ser Ser Tyr Ala Phe Asn Cys Leu Thr Thr Gly Val Gly Gln Ser Leu
20 25 30
Gly Ile Ile Asp Val Asn Glu Lys Arg Val Gln Gly Asp Val Glu Asp
35 40 45
Leu Ser Asp Ala Leu Pro Tyr Thr Ser Gln Lys Asn Ile Tyr Ala Ala
50 55 60
Ser Tyr Glu Asp Cys Lys Tyr Ala Asp Ile Ile Val Ile Thr Ala Gly
65 70 75 80
Ile Ala Gln Lys Pro Gly Gln Thr Arg Leu Gln Leu Leu Ala Ile Asn
85 90 95
Ala Lys Ile Met Lys Glu Ile Thr His Asn Ile Met Ala Ser Gly Phe
100 105 110
Asn Gly Phe Ile Leu Val Ala Ser Asn Pro Val Asp Val Leu Ala Glu
115 120 125
Leu Val Leu Gln Glu Ser Gly Leu Pro Arg Asn Gln Val Leu Gly Ser
130 135 140
Gly Thr Ala Leu Asp Ser Ala Arg Leu Arg Ser Glu Ile Gly Leu Arg
145 150 155 160
Tyr Asn Val Asp Ala Arg Ile Val His Gly Tyr Ile Met Gly Glu His
165 170 175
Gly Asp Ser Glu Phe Pro Val Trp Asp Tyr Thr Asn Ile Gly Gly Lys
180 185 190
Pro Ile Leu Asp Trp Ile Pro Lys Asp Arg Gln Asp Lys Asp Leu Pro
195 200 205
Asp Ile Ser Glu Arg Val Lys Thr Ala Ala Tyr Gly Ile Ile Glu Lys
210 215 220
Lys Gly Ala Thr Phe Tyr Gly Ile Ala Ala Ser Leu Thr Arg Leu Thr
225 230 235 240
Ser Ala Phe Leu Asn Asp Asp Arg Ala Ala Phe Ala Met Ser Val His
245 250 255
Leu Glu Gly Glu Tyr Gly Leu Ser Gly Val Ser Ile Gly Val Pro Val
260 265 270
Ile Leu Gly Ala Asn Gly Leu Glu Arg Ile Ile Glu Leu Asp Leu Asn
275 280 285
Pro Glu Asp His Lys Arg Leu Ala Asp Ser Ala Ala Ile Leu Lys Glu
290 295 300
Asn Leu Lys Lys Ala Gln Glu Ala
305 310
<210> 13
<211> 981
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(981)
<223> 干酪乳杆菌 ATCC 334 _ 基因 LSEI_2549 编码乳酸脱氢酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（GTG）处的tRNA插入Met而不是Val
<400> 13
gtg gca agt att acg gat aag gat cac caa aaa gtt att ctc gtt ggt 48
Val Ala Ser Ile Thr Asp Lys Asp His Gln Lys Val Ile Leu Val Gly
1 5 10 15
gac ggc gcc gtt ggt tca agt tat gcc tac gca atg gtt ttg caa ggt 96
Asp Gly Ala Val Gly Ser Ser Tyr Ala Tyr Ala Met Val Leu Gln Gly
20 25 30
atc gct cag gaa atc gga atc gtt gac att ttc aag gac aag aca aag 144
Ile Ala Gln Glu Ile Gly Ile Val Asp Ile Phe Lys Asp Lys Thr Lys
35 40 45
ggt gac gcg att gac ttg agc aac gcg ctc cca ttc aca agt cct aag 192
Gly Asp Ala Ile Asp Leu Ser Asn Ala Leu Pro Phe Thr Ser Pro Lys
50 55 60
aag att tat tca gct gaa tac agc gat gct aag gat gct gat ctg gtt 240
Lys Ile Tyr Ser Ala Glu Tyr Ser Asp Ala Lys Asp Ala Asp Leu Val
65 70 75 80
gtt atc aca gct ggc gct cct cag aag cct ggc gaa act cgt ttg gac 288
Val Ile Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp
85 90 95
ttg gtt aac aag aac ttg aag atc ttg aag tcc att gtt gac cca atc 336
Leu Val Asn Lys Asn Leu Lys Ile Leu Lys Ser Ile Val Asp Pro Ile
100 105 110
gtt gat tcc ggc ttt aac ggt att ttc tta gtt gct gcc aac cca gtt 384
Val Asp Ser Gly Phe Asn Gly Ile Phe Leu Val Ala Ala Asn Pro Val
115 120 125
gac att ttg acc tat gca act tgg aaa ctt tct ggc ttc ccg aag aac 432
Asp Ile Leu Thr Tyr Ala Thr Trp Lys Leu Ser Gly Phe Pro Lys Asn
130 135 140
cgg gtt gtt ggt tcc ggt act tca ctg gac acc gcc cgc ttc cgt cag 480
Arg Val Val Gly Ser Gly Thr Ser Leu Asp Thr Ala Arg Phe Arg Gln
145 150 155 160
tcc att gct gaa atg gtt aat gtt gac gct cgt tcg gtc cac gct tac 528
Ser Ile Ala Glu Met Val Asn Val Asp Ala Arg Ser Val His Ala Tyr
165 170 175
atc atg ggc gaa cat ggt gac act gaa ttc cct gta tgg tcc cac gct 576
Ile Met Gly Glu His Gly Asp Thr Glu Phe Pro Val Trp Ser His Ala
180 185 190
aac att ggt ggc gtt acc atc gct gaa tgg gtt aag gct cat cca gaa 624
Asn Ile Gly Gly Val Thr Ile Ala Glu Trp Val Lys Ala His Pro Glu
195 200 205
atc aag gaa gac aag ctt gtt aag atg ttt gaa gac gtt cgt gac gcc 672
Ile Lys Glu Asp Lys Leu Val Lys Met Phe Glu Asp Val Arg Asp Ala
210 215 220
gct tat gaa atc atc aaa ctc aag ggt gcg acc ttc tat ggt atc gca 720
Ala Tyr Glu Ile Ile Lys Leu Lys Gly Ala Thr Phe Tyr Gly Ile Ala
225 230 235 240
act gcc ctt gcc cgg att tca aag gca atc ctt aac gac gaa aat gcg 768
Thr Ala Leu Ala Arg Ile Ser Lys Ala Ile Leu Asn Asp Glu Asn Ala
245 250 255
gtt ctg cca ctt tcc gtt tac atg gat ggt caa tat ggc ttg aac gac 816
Val Leu Pro Leu Ser Val Tyr Met Asp Gly Gln Tyr Gly Leu Asn Asp
260 265 270
atc tac atc ggt act cca gct gtg atc aac cgt aat ggt atc cag aac 864
Ile Tyr Ile Gly Thr Pro Ala Val Ile Asn Arg Asn Gly Ile Gln Asn
275 280 285
atc ctg gaa atc cca ttg acc gat cac gaa gaa gaa tcc atg cag aaa 912
Ile Leu Glu Ile Pro Leu Thr Asp His Glu Glu Glu Ser Met Gln Lys
290 295 300
tct gct tct caa ttg aag aag gtt ctg acc gat gct ttc gct aag aat 960
Ser Ala Ser Gln Leu Lys Lys Val Leu Thr Asp Ala Phe Ala Lys Asn
305 310 315 320
gac atc gaa act cgt cag taa 981
Asp Ile Glu Thr Arg Gln
325
<210> 14
<211> 326
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 14
Val Ala Ser Ile Thr Asp Lys Asp His Gln Lys Val Ile Leu Val Gly
1 5 10 15
Asp Gly Ala Val Gly Ser Ser Tyr Ala Tyr Ala Met Val Leu Gln Gly
20 25 30
Ile Ala Gln Glu Ile Gly Ile Val Asp Ile Phe Lys Asp Lys Thr Lys
35 40 45
Gly Asp Ala Ile Asp Leu Ser Asn Ala Leu Pro Phe Thr Ser Pro Lys
50 55 60
Lys Ile Tyr Ser Ala Glu Tyr Ser Asp Ala Lys Asp Ala Asp Leu Val
65 70 75 80
Val Ile Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp
85 90 95
Leu Val Asn Lys Asn Leu Lys Ile Leu Lys Ser Ile Val Asp Pro Ile
100 105 110
Val Asp Ser Gly Phe Asn Gly Ile Phe Leu Val Ala Ala Asn Pro Val
115 120 125
Asp Ile Leu Thr Tyr Ala Thr Trp Lys Leu Ser Gly Phe Pro Lys Asn
130 135 140
Arg Val Val Gly Ser Gly Thr Ser Leu Asp Thr Ala Arg Phe Arg Gln
145 150 155 160
Ser Ile Ala Glu Met Val Asn Val Asp Ala Arg Ser Val His Ala Tyr
165 170 175
Ile Met Gly Glu His Gly Asp Thr Glu Phe Pro Val Trp Ser His Ala
180 185 190
Asn Ile Gly Gly Val Thr Ile Ala Glu Trp Val Lys Ala His Pro Glu
195 200 205
Ile Lys Glu Asp Lys Leu Val Lys Met Phe Glu Asp Val Arg Asp Ala
210 215 220
Ala Tyr Glu Ile Ile Lys Leu Lys Gly Ala Thr Phe Tyr Gly Ile Ala
225 230 235 240
Thr Ala Leu Ala Arg Ile Ser Lys Ala Ile Leu Asn Asp Glu Asn Ala
245 250 255
Val Leu Pro Leu Ser Val Tyr Met Asp Gly Gln Tyr Gly Leu Asn Asp
260 265 270
Ile Tyr Ile Gly Thr Pro Ala Val Ile Asn Arg Asn Gly Ile Gln Asn
275 280 285
Ile Leu Glu Ile Pro Leu Thr Asp His Glu Glu Glu Ser Met Gln Lys
290 295 300
Ser Ala Ser Gln Leu Lys Lys Val Leu Thr Asp Ala Phe Ala Lys Asn
305 310 315 320
Asp Ile Glu Thr Arg Gln
325
<210> 15
<211> 906
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(906)
<223> 干酪乳杆菌 ATCC 334_基因 LSEI_2607 编码乳酸脱氢酶
<400> 15
atg gca aga aca att ggt att att ggt att gga cac gta ggt gtg acc 48
Met Ala Arg Thr Ile Gly Ile Ile Gly Ile Gly His Val Gly Val Thr
1 5 10 15
acg gct ttt aat ctt gtc agc aaa ggg gtt gct gac aag tta gtc ttg 96
Thr Ala Phe Asn Leu Val Ser Lys Gly Val Ala Asp Lys Leu Val Leu
20 25 30
att gat aaa aag gct gaa ctt gct gaa ggc gaa agc ttt gac ttg aag 144
Ile Asp Lys Lys Ala Glu Leu Ala Glu Gly Glu Ser Phe Asp Leu Lys
35 40 45
gat gcg ctt ggc ggc ttg ccc act tat act gac att gtt gtc aac gat 192
Asp Ala Leu Gly Gly Leu Pro Thr Tyr Thr Asp Ile Val Val Asn Asp
50 55 60
tat gat gct ttg aaa gat gct gat gtg gtt att tcc gcg gtc ggc aat 240
Tyr Asp Ala Leu Lys Asp Ala Asp Val Val Ile Ser Ala Val Gly Asn
65 70 75 80
att ggc gcc att tca aat ggt gat cga att gga gaa acc aag act tca 288
Ile Gly Ala Ile Ser Asn Gly Asp Arg Ile Gly Glu Thr Lys Thr Ser
85 90 95
aaa gta gca cta gat gac gtc gct cca aaa ctg aag gcg tct ggc ttc 336
Lys Val Ala Leu Asp Asp Val Ala Pro Lys Leu Lys Ala Ser Gly Phe
100 105 110
cat ggc gtt ttg cta gat atc acc aat cca tgt gat gct gtc acg agt 384
His Gly Val Leu Leu Asp Ile Thr Asn Pro Cys Asp Ala Val Thr Ser
115 120 125
tac tgg caa tat ctc cta gat tta ccg aag tct caa att att ggg act 432
Tyr Trp Gln Tyr Leu Leu Asp Leu Pro Lys Ser Gln Ile Ile Gly Thr
130 135 140
ggg act tcg ctg gat act tat cgg atg cga cgg gcg gtt gcc gat acc 480
Gly Thr Ser Leu Asp Thr Tyr Arg Met Arg Arg Ala Val Ala Asp Thr
145 150 155 160
ttg cat gtg aat gtt gct gac gtg cgt ggt tac aat atg ggc gaa cat 528
Leu His Val Asn Val Ala Asp Val Arg Gly Tyr Asn Met Gly Glu His
165 170 175
ggc gaa tcg caa ttt acc gct tgg tca aca gta cgg gtc aat aat gaa 576
Gly Glu Ser Gln Phe Thr Ala Trp Ser Thr Val Arg Val Asn Asn Glu
180 185 190
ccg att gct gaa tat gcg aag gtt gat tac gac cag ctt gcc gat gat 624
Pro Ile Ala Glu Tyr Ala Lys Val Asp Tyr Asp Gln Leu Ala Asp Asp
195 200 205
gcg cgg gca ggc ggt tgg aag att tat cag gct aag cat tat acg agc 672
Ala Arg Ala Gly Gly Trp Lys Ile Tyr Gln Ala Lys His Tyr Thr Ser
210 215 220
tac ggt atc gcc acc atc gcg act gaa atg aca cag gcg atc atc agc 720
Tyr Gly Ile Ala Thr Ile Ala Thr Glu Met Thr Gln Ala Ile Ile Ser
225 230 235 240
gat gca cat cgc atc ttc cca tgc gct aat tac gat cca gaa ttc ggt 768
Asp Ala His Arg Ile Phe Pro Cys Ala Asn Tyr Asp Pro Glu Phe Gly
245 250 255
att gct atc ggt cat cca gcc atg atc ggt aaa caa ggt gtt atc aag 816
Ile Ala Ile Gly His Pro Ala Met Ile Gly Lys Gln Gly Val Ile Lys
260 265 270
acg cca acg ttg aag ttg aca gat gaa gag cgt gct aag tat gtt cac 864
Thr Pro Thr Leu Lys Leu Thr Asp Glu Glu Arg Ala Lys Tyr Val His
275 280 285
tct gcc ggt atc att aag gat acc ttt gaa aaa atg aag tag 906
Ser Ala Gly Ile Ile Lys Asp Thr Phe Glu Lys Met Lys
290 295 300
<210> 16
<211> 301
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 16
Met Ala Arg Thr Ile Gly Ile Ile Gly Ile Gly His Val Gly Val Thr
1 5 10 15
Thr Ala Phe Asn Leu Val Ser Lys Gly Val Ala Asp Lys Leu Val Leu
20 25 30
Ile Asp Lys Lys Ala Glu Leu Ala Glu Gly Glu Ser Phe Asp Leu Lys
35 40 45
Asp Ala Leu Gly Gly Leu Pro Thr Tyr Thr Asp Ile Val Val Asn Asp
50 55 60
Tyr Asp Ala Leu Lys Asp Ala Asp Val Val Ile Ser Ala Val Gly Asn
65 70 75 80
Ile Gly Ala Ile Ser Asn Gly Asp Arg Ile Gly Glu Thr Lys Thr Ser
85 90 95
Lys Val Ala Leu Asp Asp Val Ala Pro Lys Leu Lys Ala Ser Gly Phe
100 105 110
His Gly Val Leu Leu Asp Ile Thr Asn Pro Cys Asp Ala Val Thr Ser
115 120 125
Tyr Trp Gln Tyr Leu Leu Asp Leu Pro Lys Ser Gln Ile Ile Gly Thr
130 135 140
Gly Thr Ser Leu Asp Thr Tyr Arg Met Arg Arg Ala Val Ala Asp Thr
145 150 155 160
Leu His Val Asn Val Ala Asp Val Arg Gly Tyr Asn Met Gly Glu His
165 170 175
Gly Glu Ser Gln Phe Thr Ala Trp Ser Thr Val Arg Val Asn Asn Glu
180 185 190
Pro Ile Ala Glu Tyr Ala Lys Val Asp Tyr Asp Gln Leu Ala Asp Asp
195 200 205
Ala Arg Ala Gly Gly Trp Lys Ile Tyr Gln Ala Lys His Tyr Thr Ser
210 215 220
Tyr Gly Ile Ala Thr Ile Ala Thr Glu Met Thr Gln Ala Ile Ile Ser
225 230 235 240
Asp Ala His Arg Ile Phe Pro Cys Ala Asn Tyr Asp Pro Glu Phe Gly
245 250 255
Ile Ala Ile Gly His Pro Ala Met Ile Gly Lys Gln Gly Val Ile Lys
260 265 270
Thr Pro Thr Leu Lys Leu Thr Asp Glu Glu Arg Ala Lys Tyr Val His
275 280 285
Ser Ala Gly Ile Ile Lys Asp Thr Phe Glu Lys Met Lys
290 295 300
<210> 17
<211> 963
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(963)
<223> 植物乳杆菌 WCFS1_ 基因 lp_0537 编码乳酸脱氢酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（TTG）处的tRNA插入Met而不是Leu
<400> 17
ttg tca agc atg cca aat cat caa aaa gtt gtg tta gtc ggc gac ggc 48
Leu Ser Ser Met Pro Asn His Gln Lys Val Val Leu Val Gly Asp Gly
1 5 10 15
gct gtt ggt tct agt tac gct ttt gcc atg gca caa caa gga att gct 96
Ala Val Gly Ser Ser Tyr Ala Phe Ala Met Ala Gln Gln Gly Ile Ala
20 25 30
gaa gaa ttt gta att gtc gat gtt gtt aaa gat cgg aca aag ggt gac 144
Glu Glu Phe Val Ile Val Asp Val Val Lys Asp Arg Thr Lys Gly Asp
35 40 45
gcc ctt gat ctt gaa gac gcc caa gca ttc acc gct ccc aag aag att 192
Ala Leu Asp Leu Glu Asp Ala Gln Ala Phe Thr Ala Pro Lys Lys Ile
50 55 60
tac tca ggc gaa tat tca gat tgt aag gac gct gac tta gtt gtt att 240
Tyr Ser Gly Glu Tyr Ser Asp Cys Lys Asp Ala Asp Leu Val Val Ile
65 70 75 80
aca gcc ggt gcg cct caa aag cct ggt gaa tca cgt tta gac tta gtt 288
Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Ser Arg Leu Asp Leu Val
85 90 95
aac aag aat tta aat atc cta tca tcc att gtc aaa cca gtt gtt gac 336
Asn Lys Asn Leu Asn Ile Leu Ser Ser Ile Val Lys Pro Val Val Asp
100 105 110
tcc ggc ttt gac ggc atc ttc tta gtt gct gct aac cct gtt gac atc 384
Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ala Asn Pro Val Asp Ile
115 120 125
tta act tac gct act tgg aaa ttc tca ggt ttc cca aag gat cgt gtc 432
Leu Thr Tyr Ala Thr Trp Lys Phe Ser Gly Phe Pro Lys Asp Arg Val
130 135 140
att ggt tca ggg act tcc tta gac tct tca cgt tta cgc gtt gcg tta 480
Ile Gly Ser Gly Thr Ser Leu Asp Ser Ser Arg Leu Arg Val Ala Leu
145 150 155 160
ggc aaa caa ttc aat gtt gat cct cgt tcc gtt gat gct tac atc atg 528
Gly Lys Gln Phe Asn Val Asp Pro Arg Ser Val Asp Ala Tyr Ile Met
165 170 175
ggt gaa cac ggt gat tct gaa ttt gct gct tac tca act gca acc atc 576
Gly Glu His Gly Asp Ser Glu Phe Ala Ala Tyr Ser Thr Ala Thr Ile
180 185 190
ggg aca cgt cca gtt cgc gat gtc gct aag gaa caa ggc gtt tct gac 624
Gly Thr Arg Pro Val Arg Asp Val Ala Lys Glu Gln Gly Val Ser Asp
195 200 205
gaa gat tta gcc aag tta gaa gac ggt gtt cgt aac aaa gct tac gac 672
Glu Asp Leu Ala Lys Leu Glu Asp Gly Val Arg Asn Lys Ala Tyr Asp
210 215 220
atc atc aac ttg aag ggt gcc acg ttc tac ggt atc ggg act gct tta 720
Ile Ile Asn Leu Lys Gly Ala Thr Phe Tyr Gly Ile Gly Thr Ala Leu
225 230 235 240
atg cgg att tcc aaa gcc att tta cgt gat gaa aat gcc gtt tta cca 768
Met Arg Ile Ser Lys Ala Ile Leu Arg Asp Glu Asn Ala Val Leu Pro
245 250 255
gta ggt gcc tac atg gac ggc caa tac ggc tta aac gac att tat atc 816
Val Gly Ala Tyr Met Asp Gly Gln Tyr Gly Leu Asn Asp Ile Tyr Ile
260 265 270
ggg act ccg gct gtg att ggt gga act ggt ttg aaa caa atc atc gaa 864
Gly Thr Pro Ala Val Ile Gly Gly Thr Gly Leu Lys Gln Ile Ile Glu
275 280 285
tca cca ctt tca gct gac gaa ctc aag aag atg caa gat tcc gcc gca 912
Ser Pro Leu Ser Ala Asp Glu Leu Lys Lys Met Gln Asp Ser Ala Ala
290 295 300
act ttg aaa aaa gtg ctt aac gac ggt tta gct gaa tta gaa aat aaa 960
Thr Leu Lys Lys Val Leu Asn Asp Gly Leu Ala Glu Leu Glu Asn Lys
305 310 315 320
taa 963
<210> 18
<211> 320
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 18
Leu Ser Ser Met Pro Asn His Gln Lys Val Val Leu Val Gly Asp Gly
1 5 10 15
Ala Val Gly Ser Ser Tyr Ala Phe Ala Met Ala Gln Gln Gly Ile Ala
20 25 30
Glu Glu Phe Val Ile Val Asp Val Val Lys Asp Arg Thr Lys Gly Asp
35 40 45
Ala Leu Asp Leu Glu Asp Ala Gln Ala Phe Thr Ala Pro Lys Lys Ile
50 55 60
Tyr Ser Gly Glu Tyr Ser Asp Cys Lys Asp Ala Asp Leu Val Val Ile
65 70 75 80
Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Ser Arg Leu Asp Leu Val
85 90 95
Asn Lys Asn Leu Asn Ile Leu Ser Ser Ile Val Lys Pro Val Val Asp
100 105 110
Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ala Asn Pro Val Asp Ile
115 120 125
Leu Thr Tyr Ala Thr Trp Lys Phe Ser Gly Phe Pro Lys Asp Arg Val
130 135 140
Ile Gly Ser Gly Thr Ser Leu Asp Ser Ser Arg Leu Arg Val Ala Leu
145 150 155 160
Gly Lys Gln Phe Asn Val Asp Pro Arg Ser Val Asp Ala Tyr Ile Met
165 170 175
Gly Glu His Gly Asp Ser Glu Phe Ala Ala Tyr Ser Thr Ala Thr Ile
180 185 190
Gly Thr Arg Pro Val Arg Asp Val Ala Lys Glu Gln Gly Val Ser Asp
195 200 205
Glu Asp Leu Ala Lys Leu Glu Asp Gly Val Arg Asn Lys Ala Tyr Asp
210 215 220
Ile Ile Asn Leu Lys Gly Ala Thr Phe Tyr Gly Ile Gly Thr Ala Leu
225 230 235 240
Met Arg Ile Ser Lys Ala Ile Leu Arg Asp Glu Asn Ala Val Leu Pro
245 250 255
Val Gly Ala Tyr Met Asp Gly Gln Tyr Gly Leu Asn Asp Ile Tyr Ile
260 265 270
Gly Thr Pro Ala Val Ile Gly Gly Thr Gly Leu Lys Gln Ile Ile Glu
275 280 285
Ser Pro Leu Ser Ala Asp Glu Leu Lys Lys Met Gln Asp Ser Ala Ala
290 295 300
Thr Leu Lys Lys Val Leu Asn Asp Gly Leu Ala Glu Leu Glu Asn Lys
305 310 315 320
<210> 19
<211> 930
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(930)
<223> 植物乳杆菌 WCFS1; 基因 lp_1101 编码乳酸脱氢酶
<400> 19
atg gat aag aag caa cgc aaa gtc gta att gtt ggt gat ggc tcg gtg 48
Met Asp Lys Lys Gln Arg Lys Val Val Ile Val Gly Asp Gly Ser Val
1 5 10 15
ggt tca tca ttt gcc ttt tca ttg gtc caa aat tgc gcc cta gat gaa 96
Gly Ser Ser Phe Ala Phe Ser Leu Val Gln Asn Cys Ala Leu Asp Glu
20 25 30
ctc gtt atc gtt gac ttg gtt aaa acg cac gca gag ggg gac gtt aag 144
Leu Val Ile Val Asp Leu Val Lys Thr His Ala Glu Gly Asp Val Lys
35 40 45
gat ttg gaa gat gtt gcc gcc ttt acg aat gcg acc aac att cat acc 192
Asp Leu Glu Asp Val Ala Ala Phe Thr Asn Ala Thr Asn Ile His Thr
50 55 60
ggt gaa tat gcg gat gcg cgt gat gct gac atc gtt gtc att acg gct 240
Gly Glu Tyr Ala Asp Ala Arg Asp Ala Asp Ile Val Val Ile Thr Ala
65 70 75 80
ggt gtg cct cgt aag cct ggt gag agt cgt tta gat ttg att aac cgc 288
Gly Val Pro Arg Lys Pro Gly Glu Ser Arg Leu Asp Leu Ile Asn Arg
85 90 95
aat acg aag att ctg gaa tcc atc gtc aaa cca gtg gtt gcg agt ggt 336
Asn Thr Lys Ile Leu Glu Ser Ile Val Lys Pro Val Val Ala Ser Gly
100 105 110
ttt aat ggt tgc ttc gtt atc tca agt aat ccc gtc gat att ttg act 384
Phe Asn Gly Cys Phe Val Ile Ser Ser Asn Pro Val Asp Ile Leu Thr
115 120 125
tcg atg acg caa cgt tta tcc ggt ttt cca cgg cat cgg gtc att ggt 432
Ser Met Thr Gln Arg Leu Ser Gly Phe Pro Arg His Arg Val Ile Gly
130 135 140
acc ggg act tcc ttg gat acg gcg cgg tta cgg gtc gcc ttg gct cag 480
Thr Gly Thr Ser Leu Asp Thr Ala Arg Leu Arg Val Ala Leu Ala Gln
145 150 155 160
aag ttg aat gtt gcc acc act gca gtt gat gct gcg gta ctt gga gaa 528
Lys Leu Asn Val Ala Thr Thr Ala Val Asp Ala Ala Val Leu Gly Glu
165 170 175
cat ggt gat agt tcc atc gtt aat ttt gat gaa att atg atc aat gct 576
His Gly Asp Ser Ser Ile Val Asn Phe Asp Glu Ile Met Ile Asn Ala
180 185 190
cag ccc tta aag acg gtc aca acg gtc gat gat cag ttc aaa gct gaa 624
Gln Pro Leu Lys Thr Val Thr Thr Val Asp Asp Gln Phe Lys Ala Glu
195 200 205
atc gag caa gct gtt cgt ggt aaa ggt ggt caa atc att agt cag aag 672
Ile Glu Gln Ala Val Arg Gly Lys Gly Gly Gln Ile Ile Ser Gln Lys
210 215 220
ggg gcc acg ttc tat ggg gtc gcc gtt agt ttg atg caa atc tgc cga 720
Gly Ala Thr Phe Tyr Gly Val Ala Val Ser Leu Met Gln Ile Cys Arg
225 230 235 240
gca att ttg aac gat gaa aat gct gag ttg att gtc tcc gcc gct ttg 768
Ala Ile Leu Asn Asp Glu Asn Ala Glu Leu Ile Val Ser Ala Ala Leu
245 250 255
tct ggt caa tat ggc att aac gat ttg tac ttg ggg tca ccc gcc att 816
Ser Gly Gln Tyr Gly Ile Asn Asp Leu Tyr Leu Gly Ser Pro Ala Ile
260 265 270
att aac cgc aac ggg ctc caa aaa gtg atc gaa gct gag cta tca gat 864
Ile Asn Arg Asn Gly Leu Gln Lys Val Ile Glu Ala Glu Leu Ser Asp
275 280 285
gat gag cgt gcc cgg atg caa cat ttc gca gcc aag atg ctg acc atg 912
Asp Glu Arg Ala Arg Met Gln His Phe Ala Ala Lys Met Leu Thr Met
290 295 300
atg aat gtg gca tca taa 930
Met Asn Val Ala Ser
305
<210> 20
<211> 309
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 20
Met Asp Lys Lys Gln Arg Lys Val Val Ile Val Gly Asp Gly Ser Val
1 5 10 15
Gly Ser Ser Phe Ala Phe Ser Leu Val Gln Asn Cys Ala Leu Asp Glu
20 25 30
Leu Val Ile Val Asp Leu Val Lys Thr His Ala Glu Gly Asp Val Lys
35 40 45
Asp Leu Glu Asp Val Ala Ala Phe Thr Asn Ala Thr Asn Ile His Thr
50 55 60
Gly Glu Tyr Ala Asp Ala Arg Asp Ala Asp Ile Val Val Ile Thr Ala
65 70 75 80
Gly Val Pro Arg Lys Pro Gly Glu Ser Arg Leu Asp Leu Ile Asn Arg
85 90 95
Asn Thr Lys Ile Leu Glu Ser Ile Val Lys Pro Val Val Ala Ser Gly
100 105 110
Phe Asn Gly Cys Phe Val Ile Ser Ser Asn Pro Val Asp Ile Leu Thr
115 120 125
Ser Met Thr Gln Arg Leu Ser Gly Phe Pro Arg His Arg Val Ile Gly
130 135 140
Thr Gly Thr Ser Leu Asp Thr Ala Arg Leu Arg Val Ala Leu Ala Gln
145 150 155 160
Lys Leu Asn Val Ala Thr Thr Ala Val Asp Ala Ala Val Leu Gly Glu
165 170 175
His Gly Asp Ser Ser Ile Val Asn Phe Asp Glu Ile Met Ile Asn Ala
180 185 190
Gln Pro Leu Lys Thr Val Thr Thr Val Asp Asp Gln Phe Lys Ala Glu
195 200 205
Ile Glu Gln Ala Val Arg Gly Lys Gly Gly Gln Ile Ile Ser Gln Lys
210 215 220
Gly Ala Thr Phe Tyr Gly Val Ala Val Ser Leu Met Gln Ile Cys Arg
225 230 235 240
Ala Ile Leu Asn Asp Glu Asn Ala Glu Leu Ile Val Ser Ala Ala Leu
245 250 255
Ser Gly Gln Tyr Gly Ile Asn Asp Leu Tyr Leu Gly Ser Pro Ala Ile
260 265 270
Ile Asn Arg Asn Gly Leu Gln Lys Val Ile Glu Ala Glu Leu Ser Asp
275 280 285
Asp Glu Arg Ala Arg Met Gln His Phe Ala Ala Lys Met Leu Thr Met
290 295 300
Met Asn Val Ala Ser
305
<210> 21
<211> 963
<212> DNA
<213> 戊糖片球菌（Pediococcus pentosaceus）
<220>
<221> CDS
<222> (1)..(963)
<223> 戊糖片球菌 ATCC 25745: 基因 PEPE_1554 编码乳酸脱氢酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（TTG）处的tRNA插入Met而不是Leu
<400> 21
ttg tct aaa att caa aac cat caa aaa gtt gta tta gtc ggc gac ggc 48
Leu Ser Lys Ile Gln Asn His Gln Lys Val Val Leu Val Gly Asp Gly
1 5 10 15
gct gta ggt tca agt tat gcc ttt gca atg gca caa caa gga att gca 96
Ala Val Gly Ser Ser Tyr Ala Phe Ala Met Ala Gln Gln Gly Ile Ala
20 25 30
gaa gaa ttc gta atc gtt gat gtt gtt aag gac cgt act gtt ggt gac 144
Glu Glu Phe Val Ile Val Asp Val Val Lys Asp Arg Thr Val Gly Asp
35 40 45
gca ctt gat ctt gaa gat gct act cca ttt act gca cct aag aac att 192
Ala Leu Asp Leu Glu Asp Ala Thr Pro Phe Thr Ala Pro Lys Asn Ile
50 55 60
tac tca ggt gaa tat tct gac tgt aaa gat gca gat ctt gta gtt atc 240
Tyr Ser Gly Glu Tyr Ser Asp Cys Lys Asp Ala Asp Leu Val Val Ile
65 70 75 80
act gct ggt gct cca caa aaa cca ggt gaa aca cgt ctt gac ctt gtt 288
Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp Leu Val
85 90 95
aac aag aac tta aac att ctt tca aca atc gtt aag cca gtt gtt gac 336
Asn Lys Asn Leu Asn Ile Leu Ser Thr Ile Val Lys Pro Val Val Asp
100 105 110
tca ggc ttc gac ggt atc ttc tta gtt gct gct aac cca gtt gat atc 384
Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ala Asn Pro Val Asp Ile
115 120 125
ctt act tac gca aca tgg aaa ttc tca gga ttc cct aaa gaa aag gtt 432
Leu Thr Tyr Ala Thr Trp Lys Phe Ser Gly Phe Pro Lys Glu Lys Val
130 135 140
atc ggt tca ggt att tct ctt gat tca gca cgt cta cgt gtt gct ctt 480
Ile Gly Ser Gly Ile Ser Leu Asp Ser Ala Arg Leu Arg Val Ala Leu
145 150 155 160
ggt aag aaa ttc aac gtt agc cct gac tca gtt gat gca tac atc atg 528
Gly Lys Lys Phe Asn Val Ser Pro Asp Ser Val Asp Ala Tyr Ile Met
165 170 175
ggt gaa cat ggt gac agt gaa ttc gct gct tac tca act gct tca atc 576
Gly Glu His Gly Asp Ser Glu Phe Ala Ala Tyr Ser Thr Ala Ser Ile
180 185 190
ggt aca aag cca ttg ctt gat atc gct aaa gaa gaa ggc gtt tca act 624
Gly Thr Lys Pro Leu Leu Asp Ile Ala Lys Glu Glu Gly Val Ser Thr
195 200 205
gac gaa tta gct gaa atc gaa gac agt gtt cgt aac aaa gct tac gaa 672
Asp Glu Leu Ala Glu Ile Glu Asp Ser Val Arg Asn Lys Ala Tyr Glu
210 215 220
atc atc aac aag aag ggt gct aca ttc tac ggt gtt ggt act gct ttg 720
Ile Ile Asn Lys Lys Gly Ala Thr Phe Tyr Gly Val Gly Thr Ala Leu
225 230 235 240
atg cgt att tca aaa gct atc ttg cgt gac gaa aat gct gta ttg cct 768
Met Arg Ile Ser Lys Ala Ile Leu Arg Asp Glu Asn Ala Val Leu Pro
245 250 255
gtt ggt gct tac atg gac ggc gaa tat ggt ttg aat gac atc tac atc 816
Val Gly Ala Tyr Met Asp Gly Glu Tyr Gly Leu Asn Asp Ile Tyr Ile
260 265 270
ggt aca cct gct gtt att aac ggc caa ggt ttg aac cgt gtt atc gaa 864
Gly Thr Pro Ala Val Ile Asn Gly Gln Gly Leu Asn Arg Val Ile Glu
275 280 285
tca cca ctt agt gat gac gaa atg aag aag atg aca gat tca gct aca 912
Ser Pro Leu Ser Asp Asp Glu Met Lys Lys Met Thr Asp Ser Ala Thr
290 295 300
aca ttg aaa aaa gtt ctt aca gat ggt ttg aaa gct ctt gaa gaa aaa 960
Thr Leu Lys Lys Val Leu Thr Asp Gly Leu Lys Ala Leu Glu Glu Lys
305 310 315 320
taa 963
<210> 22
<211> 320
<212> PRT
<213> 戊糖片球菌（Pediococcus pentosaceus）
<400> 22
Leu Ser Lys Ile Gln Asn His Gln Lys Val Val Leu Val Gly Asp Gly
1 5 10 15
Ala Val Gly Ser Ser Tyr Ala Phe Ala Met Ala Gln Gln Gly Ile Ala
20 25 30
Glu Glu Phe Val Ile Val Asp Val Val Lys Asp Arg Thr Val Gly Asp
35 40 45
Ala Leu Asp Leu Glu Asp Ala Thr Pro Phe Thr Ala Pro Lys Asn Ile
50 55 60
Tyr Ser Gly Glu Tyr Ser Asp Cys Lys Asp Ala Asp Leu Val Val Ile
65 70 75 80
Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp Leu Val
85 90 95
Asn Lys Asn Leu Asn Ile Leu Ser Thr Ile Val Lys Pro Val Val Asp
100 105 110
Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ala Asn Pro Val Asp Ile
115 120 125
Leu Thr Tyr Ala Thr Trp Lys Phe Ser Gly Phe Pro Lys Glu Lys Val
130 135 140
Ile Gly Ser Gly Ile Ser Leu Asp Ser Ala Arg Leu Arg Val Ala Leu
145 150 155 160
Gly Lys Lys Phe Asn Val Ser Pro Asp Ser Val Asp Ala Tyr Ile Met
165 170 175
Gly Glu His Gly Asp Ser Glu Phe Ala Ala Tyr Ser Thr Ala Ser Ile
180 185 190
Gly Thr Lys Pro Leu Leu Asp Ile Ala Lys Glu Glu Gly Val Ser Thr
195 200 205
Asp Glu Leu Ala Glu Ile Glu Asp Ser Val Arg Asn Lys Ala Tyr Glu
210 215 220
Ile Ile Asn Lys Lys Gly Ala Thr Phe Tyr Gly Val Gly Thr Ala Leu
225 230 235 240
Met Arg Ile Ser Lys Ala Ile Leu Arg Asp Glu Asn Ala Val Leu Pro
245 250 255
Val Gly Ala Tyr Met Asp Gly Glu Tyr Gly Leu Asn Asp Ile Tyr Ile
260 265 270
Gly Thr Pro Ala Val Ile Asn Gly Gln Gly Leu Asn Arg Val Ile Glu
275 280 285
Ser Pro Leu Ser Asp Asp Glu Met Lys Lys Met Thr Asp Ser Ala Thr
290 295 300
Thr Leu Lys Lys Val Leu Thr Asp Gly Leu Lys Ala Leu Glu Glu Lys
305 310 315 320
<210> 23
<211> 996
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(996)
<223> 肠膜明串珠菌肠膜亚种（Leuconostoc mesenteroides subsp.mesenteroides） ATCC 8293: 基因
LEUM_1756 编码乳酸脱氢酶
<400> 23
atg aag att ttt gct tac ggc att cgt gat gat gaa aag cca tca ctt 48
Met Lys Ile Phe Ala Tyr Gly Ile Arg Asp Asp Glu Lys Pro Ser Leu
1 5 10 15
gaa gaa tgg aaa gcg gct aac cca gag att gaa gtg gac tac aca caa 96
Glu Glu Trp Lys Ala Ala Asn Pro Glu Ile Glu Val Asp Tyr Thr Gln
20 25 30
gaa tta ttg aca cct gaa aca gct aag ttg gct gag gga tca gat tca 144
Glu Leu Leu Thr Pro Glu Thr Ala Lys Leu Ala Glu Gly Ser Asp Ser
35 40 45
gct gtt gtt tat caa caa ttg gac tat aca cgt gaa aca ttg aca gct 192
Ala Val Val Tyr Gln Gln Leu Asp Tyr Thr Arg Glu Thr Leu Thr Ala
50 55 60
tta gct aac gtt ggt gtt act aac ttg tca ttg cgt aac gtt ggt aca 240
Leu Ala Asn Val Gly Val Thr Asn Leu Ser Leu Arg Asn Val Gly Thr
65 70 75 80
gat aac att gat ttt gat gca gca cgt gaa ttt aac ttt aac att tca 288
Asp Asn Ile Asp Phe Asp Ala Ala Arg Glu Phe Asn Phe Asn Ile Ser
85 90 95
aat gtt cct gtt tat tca cca aat gct att gca gaa cac tca atg att 336
Asn Val Pro Val Tyr Ser Pro Asn Ala Ile Ala Glu His Ser Met Ile
100 105 110
caa tta tct cgt ttg cta cgt cgc acg aaa gca ttg gat gcc aaa att 384
Gln Leu Ser Arg Leu Leu Arg Arg Thr Lys Ala Leu Asp Ala Lys Ile
115 120 125
gct aag cac gac ttg cgt tgg gca cca aca att gga cgt gaa atg cgt 432
Ala Lys His Asp Leu Arg Trp Ala Pro Thr Ile Gly Arg Glu Met Arg
130 135 140
atg caa aca gtt ggt gtt att ggt aca ggt cat att ggc cgt gtt gct 480
Met Gln Thr Val Gly Val Ile Gly Thr Gly His Ile Gly Arg Val Ala
145 150 155 160
att aac att ttg aaa ggc ttt ggg gcc aag gtt att gct tat gac aag 528
Ile Asn Ile Leu Lys Gly Phe Gly Ala Lys Val Ile Ala Tyr Asp Lys
165 170 175
tac cca aat gct gaa tta caa gca gaa ggt ttg tac gtt gac aca tta 576
Tyr Pro Asn Ala Glu Leu Gln Ala Glu Gly Leu Tyr Val Asp Thr Leu
180 185 190
gac gaa tta tat gca caa gct gat gca att tca ttg tat gtt cct ggt 624
Asp Glu Leu Tyr Ala Gln Ala Asp Ala Ile Ser Leu Tyr Val Pro Gly
195 200 205
gta cct gaa aac cat cat cta atc aat gca gat gct att gct aag atg 672
Val Pro Glu Asn His His Leu Ile Asn Ala Asp Ala Ile Ala Lys Met
210 215 220
aag gat ggt gtg gtt atc atg aac gct gcg cgt ggt aat ttg atg gac 720
Lys Asp Gly Val Val Ile Met Asn Ala Ala Arg Gly Asn Leu Met Asp
225 230 235 240
att gac gct att att gat ggt ttg aat tct ggt aag att tca gac ttc 768
Ile Asp Ala Ile Ile Asp Gly Leu Asn Ser Gly Lys Ile Ser Asp Phe
245 250 255
ggt atg gac gtt tat gaa aat gaa gtt ggc ttg ttc aat gaa gat tgg 816
Gly Met Asp Val Tyr Glu Asn Glu Val Gly Leu Phe Asn Glu Asp Trp
260 265 270
tct ggt aaa gaa ttc cca gat gct aag att gct gac ttg att gca cgc 864
Ser Gly Lys Glu Phe Pro Asp Ala Lys Ile Ala Asp Leu Ile Ala Arg
275 280 285
gaa aat gta ttg gtt acg cca cac acg gct ttc tat aca act aaa gct 912
Glu Asn Val Leu Val Thr Pro His Thr Ala Phe Tyr Thr Thr Lys Ala
290 295 300
gtt cta gaa atg gtt cac caa tca ttt gat gca gca gtt gct ttc gcc 960
Val Leu Glu Met Val His Gln Ser Phe Asp Ala Ala Val Ala Phe Ala
305 310 315 320
aag ggt gag aag cca gct att gct gtt gaa tat taa 996
Lys Gly Glu Lys Pro Ala Ile Ala Val Glu Tyr
325 330
<210> 24
<211> 331
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 24
Met Lys Ile Phe Ala Tyr Gly Ile Arg Asp Asp Glu Lys Pro Ser Leu
1 5 10 15
Glu Glu Trp Lys Ala Ala Asn Pro Glu Ile Glu Val Asp Tyr Thr Gln
20 25 30
Glu Leu Leu Thr Pro Glu Thr Ala Lys Leu Ala Glu Gly Ser Asp Ser
35 40 45
Ala Val Val Tyr Gln Gln Leu Asp Tyr Thr Arg Glu Thr Leu Thr Ala
50 55 60
Leu Ala Asn Val Gly Val Thr Asn Leu Ser Leu Arg Asn Val Gly Thr
65 70 75 80
Asp Asn Ile Asp Phe Asp Ala Ala Arg Glu Phe Asn Phe Asn Ile Ser
85 90 95
Asn Val Pro Val Tyr Ser Pro Asn Ala Ile Ala Glu His Ser Met Ile
100 105 110
Gln Leu Ser Arg Leu Leu Arg Arg Thr Lys Ala Leu Asp Ala Lys Ile
115 120 125
Ala Lys His Asp Leu Arg Trp Ala Pro Thr Ile Gly Arg Glu Met Arg
130 135 140
Met Gln Thr Val Gly Val Ile Gly Thr Gly His Ile Gly Arg Val Ala
145 150 155 160
Ile Asn Ile Leu Lys Gly Phe Gly Ala Lys Val Ile Ala Tyr Asp Lys
165 170 175
Tyr Pro Asn Ala Glu Leu Gln Ala Glu Gly Leu Tyr Val Asp Thr Leu
180 185 190
Asp Glu Leu Tyr Ala Gln Ala Asp Ala Ile Ser Leu Tyr Val Pro Gly
195 200 205
Val Pro Glu Asn His His Leu Ile Asn Ala Asp Ala Ile Ala Lys Met
210 215 220
Lys Asp Gly Val Val Ile Met Asn Ala Ala Arg Gly Asn Leu Met Asp
225 230 235 240
Ile Asp Ala Ile Ile Asp Gly Leu Asn Ser Gly Lys Ile Ser Asp Phe
245 250 255
Gly Met Asp Val Tyr Glu Asn Glu Val Gly Leu Phe Asn Glu Asp Trp
260 265 270
Ser Gly Lys Glu Phe Pro Asp Ala Lys Ile Ala Asp Leu Ile Ala Arg
275 280 285
Glu Asn Val Leu Val Thr Pro His Thr Ala Phe Tyr Thr Thr Lys Ala
290 295 300
Val Leu Glu Met Val His Gln Ser Phe Asp Ala Ala Val Ala Phe Ala
305 310 315 320
Lys Gly Glu Lys Pro Ala Ile Ala Val Glu Tyr
325 330
<210> 25
<211> 990
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(990)
<223> 肠膜明串珠菌肠膜亚种 ATCC 8293: 基因
LEUM_2043 编码乳酸脱氢酶
<400> 25
atg aaa att tta atg tac aat aca gtt tca gac gaa atg cca tat gtt 48
Met Lys Ile Leu Met Tyr Asn Thr Val Ser Asp Glu Met Pro Tyr Val
1 5 10 15
gaa aaa tgg act gaa aaa aca ggc cac gaa gta gtg acc att gat caa 96
Glu Lys Trp Thr Glu Lys Thr Gly His Glu Val Val Thr Ile Asp Gln
20 25 30
cca ctt aac gaa acg acg gtg gat cgc gct aaa ggt ttt gat gca att 144
Pro Leu Asn Glu Thr Thr Val Asp Arg Ala Lys Gly Phe Asp Ala Ile
35 40 45
acc aca caa caa aca aca gcc att aat gca cca gtt tat gcg caa cta 192
Thr Thr Gln Gln Thr Thr Ala Ile Asn Ala Pro Val Tyr Ala Gln Leu
50 55 60
gct caa ttc aat att aag caa atc gct att cga cag gtt ggc tat gat 240
Ala Gln Phe Asn Ile Lys Gln Ile Ala Ile Arg Gln Val Gly Tyr Asp
65 70 75 80
gtg ttg gac ttg cca gaa gcc ttt gct cac aat att cgt ttg tct aat 288
Val Leu Asp Leu Pro Glu Ala Phe Ala His Asn Ile Arg Leu Ser Asn
85 90 95
gtt gca gcc tat tca ccc cga gca att gca gaa tac acg cta aca cag 336
Val Ala Ala Tyr Ser Pro Arg Ala Ile Ala Glu Tyr Thr Leu Thr Gln
100 105 110
ctt ttt aat tta att cga cac aat aaa aag ttt gat cgc gcc atg aat 384
Leu Phe Asn Leu Ile Arg His Asn Lys Lys Phe Asp Arg Ala Met Asn
115 120 125
acg ggt gac tat act tgg gct gct ggt ggt caa gcg aga gaa att cat 432
Thr Gly Asp Tyr Thr Trp Ala Ala Gly Gly Gln Ala Arg Glu Ile His
130 135 140
gat tta act gtt gct gtg att ggt gta ggt cga att ggg aca gcc ctt 480
Asp Leu Thr Val Ala Val Ile Gly Val Gly Arg Ile Gly Thr Ala Leu
145 150 155 160
gca gaa atg ctt cac gcg ctg ggc gca aca gtg tta ggt gtg gac cca 528
Ala Glu Met Leu His Ala Leu Gly Ala Thr Val Leu Gly Val Asp Pro
165 170 175
ata tat cgt gcc cag aac gaa cca ttt gta act tat acg aca tta gat 576
Ile Tyr Arg Ala Gln Asn Glu Pro Phe Val Thr Tyr Thr Thr Leu Asp
180 185 190
gat gct ttg tct cga gcg gat gtt att agt ttt cat aca cca tta aat 624
Asp Ala Leu Ser Arg Ala Asp Val Ile Ser Phe His Thr Pro Leu Asn
195 200 205
gac gag acg cgc tat atg gct gat gaa aat ttc ttt gct aaa att cag 672
Asp Glu Thr Arg Tyr Met Ala Asp Glu Asn Phe Phe Ala Lys Ile Gln
210 215 220
cca gga aca att atg ttg aat ttt gcg cgt ggt gaa att gtt gac atg 720
Pro Gly Thr Ile Met Leu Asn Phe Ala Arg Gly Glu Ile Val Asp Met
225 230 235 240
gtt gag ctt gaa aaa gca ctc gaa tca aag tta ttg gct ggt gca gca 768
Val Glu Leu Glu Lys Ala Leu Glu Ser Lys Leu Leu Ala Gly Ala Ala
245 250 255
ttt gat gtg aca cca aat gaa aat gat ttt atg aac caa aag caa gat 816
Phe Asp Val Thr Pro Asn Glu Asn Asp Phe Met Asn Gln Lys Gln Asp
260 265 270
ata acg gcg cta cca gca gat gta aag cgc tta atc gaa tat gac aac 864
Ile Thr Ala Leu Pro Ala Asp Val Lys Arg Leu Ile Glu Tyr Asp Asn
275 280 285
ttt cta ctg tca cca cat att gct ttt tat aca aac aca gca ata aaa 912
Phe Leu Leu Ser Pro His Ile Ala Phe Tyr Thr Asn Thr Ala Ile Lys
290 295 300
aat atg gtg gaa atg gct tta aat gat gcc gta acg atg gta aat ggc 960
Asn Met Val Glu Met Ala Leu Asn Asp Ala Val Thr Met Val Asn Gly
305 310 315 320
ggc gtt act gaa aac gaa att ttg cgg taa 990
Gly Val Thr Glu Asn Glu Ile Leu Arg
325
<210> 26
<211> 329
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 26
Met Lys Ile Leu Met Tyr Asn Thr Val Ser Asp Glu Met Pro Tyr Val
1 5 10 15
Glu Lys Trp Thr Glu Lys Thr Gly His Glu Val Val Thr Ile Asp Gln
20 25 30
Pro Leu Asn Glu Thr Thr Val Asp Arg Ala Lys Gly Phe Asp Ala Ile
35 40 45
Thr Thr Gln Gln Thr Thr Ala Ile Asn Ala Pro Val Tyr Ala Gln Leu
50 55 60
Ala Gln Phe Asn Ile Lys Gln Ile Ala Ile Arg Gln Val Gly Tyr Asp
65 70 75 80
Val Leu Asp Leu Pro Glu Ala Phe Ala His Asn Ile Arg Leu Ser Asn
85 90 95
Val Ala Ala Tyr Ser Pro Arg Ala Ile Ala Glu Tyr Thr Leu Thr Gln
100 105 110
Leu Phe Asn Leu Ile Arg His Asn Lys Lys Phe Asp Arg Ala Met Asn
115 120 125
Thr Gly Asp Tyr Thr Trp Ala Ala Gly Gly Gln Ala Arg Glu Ile His
130 135 140
Asp Leu Thr Val Ala Val Ile Gly Val Gly Arg Ile Gly Thr Ala Leu
145 150 155 160
Ala Glu Met Leu His Ala Leu Gly Ala Thr Val Leu Gly Val Asp Pro
165 170 175
Ile Tyr Arg Ala Gln Asn Glu Pro Phe Val Thr Tyr Thr Thr Leu Asp
180 185 190
Asp Ala Leu Ser Arg Ala Asp Val Ile Ser Phe His Thr Pro Leu Asn
195 200 205
Asp Glu Thr Arg Tyr Met Ala Asp Glu Asn Phe Phe Ala Lys Ile Gln
210 215 220
Pro Gly Thr Ile Met Leu Asn Phe Ala Arg Gly Glu Ile Val Asp Met
225 230 235 240
Val Glu Leu Glu Lys Ala Leu Glu Ser Lys Leu Leu Ala Gly Ala Ala
245 250 255
Phe Asp Val Thr Pro Asn Glu Asn Asp Phe Met Asn Gln Lys Gln Asp
260 265 270
Ile Thr Ala Leu Pro Ala Asp Val Lys Arg Leu Ile Glu Tyr Asp Asn
275 280 285
Phe Leu Leu Ser Pro His Ile Ala Phe Tyr Thr Asn Thr Ala Ile Lys
290 295 300
Asn Met Val Glu Met Ala Leu Asn Asp Ala Val Thr Met Val Asn Gly
305 310 315 320
Gly Val Thr Glu Asn Glu Ile Leu Arg
325
<210> 27
<211> 987
<212> DNA
<213> 嗜热链球菌（Streptococcus thermophilus）
<220>
<221> CDS
<222> (1)..(987)
<223> 嗜热链球菌 CNRZ1066: 基因 str1280编码乳酸脱氢酶
<400> 27
atg act gca act aaa cta cac aaa aaa gtc atc ctt gtt ggt gac ggt 48
Met Thr Ala Thr Lys Leu His Lys Lys Val Ile Leu Val Gly Asp Gly
1 5 10 15
gcc gta ggt tca tct tac gct ttc gca ctt gta aac caa ggt atc gct 96
Ala Val Gly Ser Ser Tyr Ala Phe Ala Leu Val Asn Gln Gly Ile Ala
20 25 30
caa gaa cta ggt atc atc gaa att cca caa tta ttt gaa aaa gcc gtt 144
Gln Glu Leu Gly Ile Ile Glu Ile Pro Gln Leu Phe Glu Lys Ala Val
35 40 45
ggt gat gcg ctt gac ctt agc cac gca ctt cct ttc act tca cct aaa 192
Gly Asp Ala Leu Asp Leu Ser His Ala Leu Pro Phe Thr Ser Pro Lys
50 55 60
aaa atc tat gca gct aaa tat gaa gac tgt gcg gat gct gac ctt gta 240
Lys Ile Tyr Ala Ala Lys Tyr Glu Asp Cys Ala Asp Ala Asp Leu Val
65 70 75 80
gtt atc act gct ggt gct cct caa aaa cca ggt gag act cgt ctt gat 288
Val Ile Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp
85 90 95
ctt gtt ggt aaa aac ctt gca atc aac aaa tca atc gtt act caa gtt 336
Leu Val Gly Lys Asn Leu Ala Ile Asn Lys Ser Ile Val Thr Gln Val
100 105 110
gtt gaa tca gga ttc aac ggt att ttc ctt gta gct gct aac cca gta 384
Val Glu Ser Gly Phe Asn Gly Ile Phe Leu Val Ala Ala Asn Pro Val
115 120 125
gac gta ttg act tac tct aca tgg aag ttc tca gga ttc cct aaa gaa 432
Asp Val Leu Thr Tyr Ser Thr Trp Lys Phe Ser Gly Phe Pro Lys Glu
130 135 140
cgc gtt atc ggt tca ggt act tca ctt gac tca gct cgt ttc cgt caa 480
Arg Val Ile Gly Ser Gly Thr Ser Leu Asp Ser Ala Arg Phe Arg Gln
145 150 155 160
gca ctt gct gaa aaa ctt aat gtc gat gct cgt tca gtt cac gct tac 528
Ala Leu Ala Glu Lys Leu Asn Val Asp Ala Arg Ser Val His Ala Tyr
165 170 175
atc atg ggt gaa cac ggc gac tca gag ttt gcg gtt tgg tca cac gct 576
Ile Met Gly Glu His Gly Asp Ser Glu Phe Ala Val Trp Ser His Ala
180 185 190
aac atc gcc ggt gta aac ctt gaa gag ttc ctt aaa gac aaa gaa aac 624
Asn Ile Ala Gly Val Asn Leu Glu Glu Phe Leu Lys Asp Lys Glu Asn
195 200 205
gtt caa gaa gct gaa tta gtt gaa ttg ttc gaa ggt gtt cgt gat gca 672
Val Gln Glu Ala Glu Leu Val Glu Leu Phe Glu Gly Val Arg Asp Ala
210 215 220
gct tac aca att atc aac aaa aaa ggt gct aca tac tac ggt atc gca 720
Ala Tyr Thr Ile Ile Asn Lys Lys Gly Ala Thr Tyr Tyr Gly Ile Ala
225 230 235 240
gta gcc ctt gct cgt atc act aaa gct atc ctt gac gat gaa aat gca 768
Val Ala Leu Ala Arg Ile Thr Lys Ala Ile Leu Asp Asp Glu Asn Ala
245 250 255
gta ctt cca ttg tct gta ttc caa gaa ggt caa tat ggt gta aac aac 816
Val Leu Pro Leu Ser Val Phe Gln Glu Gly Gln Tyr Gly Val Asn Asn
260 265 270
atc ttt atc ggt caa cct gcc att gta ggt gca cac ggt atc gta cgt 864
Ile Phe Ile Gly Gln Pro Ala Ile Val Gly Ala His Gly Ile Val Arg
275 280 285
cca gta aac atc cca ttg aac gat gct gaa caa caa aag atg aag gct 912
Pro Val Asn Ile Pro Leu Asn Asp Ala Glu Gln Gln Lys Met Lys Ala
290 295 300
tct gcc gat gaa ttg caa gct atc att gat gaa gca tgg aaa aac cct 960
Ser Ala Asp Glu Leu Gln Ala Ile Ile Asp Glu Ala Trp Lys Asn Pro
305 310 315 320
gaa ttc caa gaa gct tca aaa aac taa 987
Glu Phe Gln Glu Ala Ser Lys Asn
325
<210> 28
<211> 328
<212> PRT
<213> 嗜热链球菌（Streptococcus thermophilus）
<400> 28
Met Thr Ala Thr Lys Leu His Lys Lys Val Ile Leu Val Gly Asp Gly
1 5 10 15
Ala Val Gly Ser Ser Tyr Ala Phe Ala Leu Val Asn Gln Gly Ile Ala
20 25 30
Gln Glu Leu Gly Ile Ile Glu Ile Pro Gln Leu Phe Glu Lys Ala Val
35 40 45
Gly Asp Ala Leu Asp Leu Ser His Ala Leu Pro Phe Thr Ser Pro Lys
50 55 60
Lys Ile Tyr Ala Ala Lys Tyr Glu Asp Cys Ala Asp Ala Asp Leu Val
65 70 75 80
Val Ile Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp
85 90 95
Leu Val Gly Lys Asn Leu Ala Ile Asn Lys Ser Ile Val Thr Gln Val
100 105 110
Val Glu Ser Gly Phe Asn Gly Ile Phe Leu Val Ala Ala Asn Pro Val
115 120 125
Asp Val Leu Thr Tyr Ser Thr Trp Lys Phe Ser Gly Phe Pro Lys Glu
130 135 140
Arg Val Ile Gly Ser Gly Thr Ser Leu Asp Ser Ala Arg Phe Arg Gln
145 150 155 160
Ala Leu Ala Glu Lys Leu Asn Val Asp Ala Arg Ser Val His Ala Tyr
165 170 175
Ile Met Gly Glu His Gly Asp Ser Glu Phe Ala Val Trp Ser His Ala
180 185 190
Asn Ile Ala Gly Val Asn Leu Glu Glu Phe Leu Lys Asp Lys Glu Asn
195 200 205
Val Gln Glu Ala Glu Leu Val Glu Leu Phe Glu Gly Val Arg Asp Ala
210 215 220
Ala Tyr Thr Ile Ile Asn Lys Lys Gly Ala Thr Tyr Tyr Gly Ile Ala
225 230 235 240
Val Ala Leu Ala Arg Ile Thr Lys Ala Ile Leu Asp Asp Glu Asn Ala
245 250 255
Val Leu Pro Leu Ser Val Phe Gln Glu Gly Gln Tyr Gly Val Asn Asn
260 265 270
Ile Phe Ile Gly Gln Pro Ala Ile Val Gly Ala His Gly Ile Val Arg
275 280 285
Pro Val Asn Ile Pro Leu Asn Asp Ala Glu Gln Gln Lys Met Lys Ala
290 295 300
Ser Ala Asp Glu Leu Gln Ala Ile Ile Asp Glu Ala Trp Lys Asn Pro
305 310 315 320
Glu Phe Gln Glu Ala Ser Lys Asn
325
<210> 29
<211> 960
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(960)
<223> 酒类酒球菌 PSU-1: 基因 OEOE_0025编码乳酸脱氢酶
<400> 29
atg aaa aat gtt ctc atc aca gcg gag ctt cct caa gtt gca aat agt 48
Met Lys Asn Val Leu Ile Thr Ala Glu Leu Pro Gln Val Ala Asn Ser
1 5 10 15
ata ctt caa caa gcc ggc cta aat gtc gaa att ttt acg ggt gat aaa 96
Ile Leu Gln Gln Ala Gly Leu Asn Val Glu Ile Phe Thr Gly Asp Lys
20 25 30
tta att aca aag aag gaa tta ctt gat aaa gtc aaa gat aaa gat ttt 144
Leu Ile Thr Lys Lys Glu Leu Leu Asp Lys Val Lys Asp Lys Asp Phe
35 40 45
tta att acc tct cta tca aca gat gtt gat gaa gac gta ata aat gca 192
Leu Ile Thr Ser Leu Ser Thr Asp Val Asp Glu Asp Val Ile Asn Ala
50 55 60
gct cca aaa tta aaa tta att gcg aac ttt gga gcc gga ttc aat aac 240
Ala Pro Lys Leu Lys Leu Ile Ala Asn Phe Gly Ala Gly Phe Asn Asn
65 70 75 80
atc gac ata aat agt gct cga gca aaa gaa ata tct gtt aca aac act 288
Ile Asp Ile Asn Ser Ala Arg Ala Lys Glu Ile Ser Val Thr Asn Thr
85 90 95
cct ttt gtc tcg act act tca gta gcc gag gtc aca gca gga cta att 336
Pro Phe Val Ser Thr Thr Ser Val Ala Glu Val Thr Ala Gly Leu Ile
100 105 110
att tcc cta tcg cat cgt ttg gtt gaa gga gat aat tta atg cac gat 384
Ile Ser Leu Ser His Arg Leu Val Glu Gly Asp Asn Leu Met His Asp
115 120 125
caa gga ttc aat ggc tgg agt ccc tta ttc ttt tta ggc cat gaa cta 432
Gln Gly Phe Asn Gly Trp Ser Pro Leu Phe Phe Leu Gly His Glu Leu
130 135 140
tct aaa aaa aca tta ggg atc atc ggc atg ggt cag att ggc aag gct 480
Ser Lys Lys Thr Leu Gly Ile Ile Gly Met Gly Gln Ile Gly Lys Ala
145 150 155 160
ttg gcc aaa cgt atg cag gac ttt gat atg aaa att gtt tac aca caa 528
Leu Ala Lys Arg Met Gln Asp Phe Asp Met Lys Ile Val Tyr Thr Gln
165 170 175
agg cat cac tta aaa gaa gat gag gaa aaa gaa ctt cat gct gtt ttc 576
Arg His His Leu Lys Glu Asp Glu Glu Lys Glu Leu His Ala Val Phe
180 185 190
ttg gaa aaa gac gaa tta att aaa caa agc gat gta att agc ttg cat 624
Leu Glu Lys Asp Glu Leu Ile Lys Gln Ser Asp Val Ile Ser Leu His
195 200 205
ttg cca cta acg aaa aat acc cat cac ttg ttg ggt gct aaa gaa ttt 672
Leu Pro Leu Thr Lys Asn Thr His His Leu Leu Gly Ala Lys Glu Phe
210 215 220
gca act atg aaa aaa act tct ttc cta att aac gct gct cgt gga ccg 720
Ala Thr Met Lys Lys Thr Ser Phe Leu Ile Asn Ala Ala Arg Gly Pro
225 230 235 240
tta att gac gaa aat gca ttg cta cag tcc cta aaa gaa aag cag ctt 768
Leu Ile Asp Glu Asn Ala Leu Leu Gln Ser Leu Lys Glu Lys Gln Leu
245 250 255
gct gga gct gct tta gat gtt tat gaa cat gaa cca aaa gtt gat gat 816
Ala Gly Ala Ala Leu Asp Val Tyr Glu His Glu Pro Lys Val Asp Asp
260 265 270
caa ttt aaa cag atg aaa aat gtt att ctg act ccc cat atc ggc aat 864
Gln Phe Lys Gln Met Lys Asn Val Ile Leu Thr Pro His Ile Gly Asn
275 280 285
gca acc atc gaa gcc cgt aat gca atg gct gaa gtc gtc gcc aaa aat 912
Ala Thr Ile Glu Ala Arg Asn Ala Met Ala Glu Val Val Ala Lys Asn
290 295 300
gtg gta tca gtg tta aat ggt gaa aaa ccg aaa tat atc gtt aat taa 960
Val Val Ser Val Leu Asn Gly Glu Lys Pro Lys Tyr Ile Val Asn
305 310 315
<210> 30
<211> 319
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 30
Met Lys Asn Val Leu Ile Thr Ala Glu Leu Pro Gln Val Ala Asn Ser
1 5 10 15
Ile Leu Gln Gln Ala Gly Leu Asn Val Glu Ile Phe Thr Gly Asp Lys
20 25 30
Leu Ile Thr Lys Lys Glu Leu Leu Asp Lys Val Lys Asp Lys Asp Phe
35 40 45
Leu Ile Thr Ser Leu Ser Thr Asp Val Asp Glu Asp Val Ile Asn Ala
50 55 60
Ala Pro Lys Leu Lys Leu Ile Ala Asn Phe Gly Ala Gly Phe Asn Asn
65 70 75 80
Ile Asp Ile Asn Ser Ala Arg Ala Lys Glu Ile Ser Val Thr Asn Thr
85 90 95
Pro Phe Val Ser Thr Thr Ser Val Ala Glu Val Thr Ala Gly Leu Ile
100 105 110
Ile Ser Leu Ser His Arg Leu Val Glu Gly Asp Asn Leu Met His Asp
115 120 125
Gln Gly Phe Asn Gly Trp Ser Pro Leu Phe Phe Leu Gly His Glu Leu
130 135 140
Ser Lys Lys Thr Leu Gly Ile Ile Gly Met Gly Gln Ile Gly Lys Ala
145 150 155 160
Leu Ala Lys Arg Met Gln Asp Phe Asp Met Lys Ile Val Tyr Thr Gln
165 170 175
Arg His His Leu Lys Glu Asp Glu Glu Lys Glu Leu His Ala Val Phe
180 185 190
Leu Glu Lys Asp Glu Leu Ile Lys Gln Ser Asp Val Ile Ser Leu His
195 200 205
Leu Pro Leu Thr Lys Asn Thr His His Leu Leu Gly Ala Lys Glu Phe
210 215 220
Ala Thr Met Lys Lys Thr Ser Phe Leu Ile Asn Ala Ala Arg Gly Pro
225 230 235 240
Leu Ile Asp Glu Asn Ala Leu Leu Gln Ser Leu Lys Glu Lys Gln Leu
245 250 255
Ala Gly Ala Ala Leu Asp Val Tyr Glu His Glu Pro Lys Val Asp Asp
260 265 270
Gln Phe Lys Gln Met Lys Asn Val Ile Leu Thr Pro His Ile Gly Asn
275 280 285
Ala Thr Ile Glu Ala Arg Asn Ala Met Ala Glu Val Val Ala Lys Asn
290 295 300
Val Val Ser Val Leu Asn Gly Glu Lys Pro Lys Tyr Ile Val Asn
305 310 315
<210> 31
<211> 978
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(975)
<223> 酒类酒球菌 PSU-1: 基因 OEOE_1709编码乳酸脱氢酶
<400> 31
atg tat gct gtt ttc gaa gat gaa gta cct tat att gaa gag ttt gcc 48
Met Tyr Ala Val Phe Glu Asp Glu Val Pro Tyr Ile Glu Glu Phe Ala
1 5 10 15
aat cgt act ggc cat gaa atc gtt cag gaa acc gat tat tta tcc gaa 96
Asn Arg Thr Gly His Glu Ile Val Gln Glu Thr Asp Tyr Leu Ser Glu
20 25 30
aaa aat gtc gat ttg gca gcc ggt ttc gat gcg gtt gtt acc caa cag 144
Lys Asn Val Asp Leu Ala Ala Gly Phe Asp Ala Val Val Thr Gln Gln
35 40 45
acg gtc aac att cct gac atg gtg tat tct cgt ctg gct gaa tta ggt 192
Thr Val Asn Ile Pro Asp Met Val Tyr Ser Arg Leu Ala Glu Leu Gly
50 55 60
atc agg caa atc agt atc cgt cag gtt ggt tac gat att tta aat atc 240
Ile Arg Gln Ile Ser Ile Arg Gln Val Gly Tyr Asp Ile Leu Asn Ile
65 70 75 80
gat gcg att gat aaa gcc gga ctg cgt gcg tca aat gtc gca gct tat 288
Asp Ala Ile Asp Lys Ala Gly Leu Arg Ala Ser Asn Val Ala Ala Tyr
85 90 95
tcg cca aga gcg att gcc gaa tac aca ttg aca caa ctt tta aac ctg 336
Ser Pro Arg Ala Ile Ala Glu Tyr Thr Leu Thr Gln Leu Leu Asn Leu
100 105 110
atc cgt gac aac aag aga tat tat cag gcc aca gaa tcg ggt aat tgg 384
Ile Arg Asp Asn Lys Arg Tyr Tyr Gln Ala Thr Glu Ser Gly Asn Trp
115 120 125
tta tgg tct gca gcc cct cag ggg aaa gaa atc cat gat cta acg gtc 432
Leu Trp Ser Ala Ala Pro Gln Gly Lys Glu Ile His Asp Leu Thr Val
130 135 140
gct gtt atc ggc gct gga cgt att ggt tcg gct ctg gct gag atg ctt 480
Ala Val Ile Gly Ala Gly Arg Ile Gly Ser Ala Leu Ala Glu Met Leu
145 150 155 160
cat gta ctt ggc gcg aag gtt ttg gca gtt gat ccg gtt tac cat gct 528
His Val Leu Gly Ala Lys Val Leu Ala Val Asp Pro Val Tyr His Ala
165 170 175
caa aat gaa gct ttt ttg gat tat aca aat ctt gac gat gct ttg aaa 576
Gln Asn Glu Ala Phe Leu Asp Tyr Thr Asn Leu Asp Asp Ala Leu Lys
180 185 190
agg gct gat gtt atc act ttc cat acg cct tta aac aag cag acc aat 624
Arg Ala Asp Val Ile Thr Phe His Thr Pro Leu Asn Lys Gln Thr Asn
195 200 205
ggt atg gcc gac aaa gat ttt ttt caa aaa gtt aaa aag aat gca tac 672
Gly Met Ala Asp Lys Asp Phe Phe Gln Lys Val Lys Lys Asn Ala Tyr
210 215 220
att tta aac ttt gcc cgc gga ggg att att caa act tcg gct tta ttg 720
Ile Leu Asn Phe Ala Arg Gly Gly Ile Ile Gln Thr Ser Ala Leu Leu
225 230 235 240
gaa aat ttg cgt agc gga aaa tta gct gga gca gct ctg gat gtt ttg 768
Glu Asn Leu Arg Ser Gly Lys Leu Ala Gly Ala Ala Leu Asp Val Leu
245 250 255
ccc aat gaa acc gag ttt atg ggc aaa atc caa aat cct gac aaa ctt 816
Pro Asn Glu Thr Glu Phe Met Gly Lys Ile Gln Asn Pro Asp Lys Leu
260 265 270
ccg gct gat gtt cag gaa tta atg tcg agg gac aac gtt tta ctg tcg 864
Pro Ala Asp Val Gln Glu Leu Met Ser Arg Asp Asn Val Leu Leu Ser
275 280 285
cca cac gtg gct ttt tat acg aac ctg gcc gtt aaa aat atg gtt gaa 912
Pro His Val Ala Phe Tyr Thr Asn Leu Ala Val Lys Asn Met Val Glu
290 295 300
ata gct tta aac gat gcg att gcc ctt tct caa ggc aaa cat atc gag 960
Ile Ala Leu Asn Asp Ala Ile Ala Leu Ser Gln Gly Lys His Ile Glu
305 310 315 320
aat gaa att ctt tat taa 978
Asn Glu Ile Leu Tyr
325
<210> 32
<211> 325
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 32
Met Tyr Ala Val Phe Glu Asp Glu Val Pro Tyr Ile Glu Glu Phe Ala
1 5 10 15
Asn Arg Thr Gly His Glu Ile Val Gln Glu Thr Asp Tyr Leu Ser Glu
20 25 30
Lys Asn Val Asp Leu Ala Ala Gly Phe Asp Ala Val Val Thr Gln Gln
35 40 45
Thr Val Asn Ile Pro Asp Met Val Tyr Ser Arg Leu Ala Glu Leu Gly
50 55 60
Ile Arg Gln Ile Ser Ile Arg Gln Val Gly Tyr Asp Ile Leu Asn Ile
65 70 75 80
Asp Ala Ile Asp Lys Ala Gly Leu Arg Ala Ser Asn Val Ala Ala Tyr
85 90 95
Ser Pro Arg Ala Ile Ala Glu Tyr Thr Leu Thr Gln Leu Leu Asn Leu
100 105 110
Ile Arg Asp Asn Lys Arg Tyr Tyr Gln Ala Thr Glu Ser Gly Asn Trp
115 120 125
Leu Trp Ser Ala Ala Pro Gln Gly Lys Glu Ile His Asp Leu Thr Val
130 135 140
Ala Val Ile Gly Ala Gly Arg Ile Gly Ser Ala Leu Ala Glu Met Leu
145 150 155 160
His Val Leu Gly Ala Lys Val Leu Ala Val Asp Pro Val Tyr His Ala
165 170 175
Gln Asn Glu Ala Phe Leu Asp Tyr Thr Asn Leu Asp Asp Ala Leu Lys
180 185 190
Arg Ala Asp Val Ile Thr Phe His Thr Pro Leu Asn Lys Gln Thr Asn
195 200 205
Gly Met Ala Asp Lys Asp Phe Phe Gln Lys Val Lys Lys Asn Ala Tyr
210 215 220
Ile Leu Asn Phe Ala Arg Gly Gly Ile Ile Gln Thr Ser Ala Leu Leu
225 230 235 240
Glu Asn Leu Arg Ser Gly Lys Leu Ala Gly Ala Ala Leu Asp Val Leu
245 250 255
Pro Asn Glu Thr Glu Phe Met Gly Lys Ile Gln Asn Pro Asp Lys Leu
260 265 270
Pro Ala Asp Val Gln Glu Leu Met Ser Arg Asp Asn Val Leu Leu Ser
275 280 285
Pro His Val Ala Phe Tyr Thr Asn Leu Ala Val Lys Asn Met Val Glu
290 295 300
Ile Ala Leu Asn Asp Ala Ile Ala Leu Ser Gln Gly Lys His Ile Glu
305 310 315 320
Asn Glu Ile Leu Tyr
325
<210> 33
<211> 939
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(939)
<223> 凝结芽孢杆菌 36D1: 基因 Bcoa_0653 编码乳酸脱氢酶
<400> 33
atg aaa aag gtc aat cgt att gca gtg gtt gga acg ggt gca gtt ggt 48
Met Lys Lys Val Asn Arg Ile Ala Val Val Gly Thr Gly Ala Val Gly
1 5 10 15
aca agt tac tgc tac gcc atg att aat cag ggt gtt gca gaa gag ctt 96
Thr Ser Tyr Cys Tyr Ala Met Ile Asn Gln Gly Val Ala Glu Glu Leu
20 25 30
gtt tta atc gat att aac gaa gca aaa gca gaa ggg gaa gcc atg gac 144
Val Leu Ile Asp Ile Asn Glu Ala Lys Ala Glu Gly Glu Ala Met Asp
35 40 45
ctg aac cac ggc ctg cca ttt gcg cct acg ccg acc cgc gtt tgg aaa 192
Leu Asn His Gly Leu Pro Phe Ala Pro Thr Pro Thr Arg Val Trp Lys
50 55 60
ggc gat tat tcc gat tgc ggc act gcc gat ctt gtt gtc att acg gca 240
Gly Asp Tyr Ser Asp Cys Gly Thr Ala Asp Leu Val Val Ile Thr Ala
65 70 75 80
ggt tcc ccg caa aaa ccg ggc gaa aca agg ctt gat ctt gtt gcc aaa 288
Gly Ser Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp Leu Val Ala Lys
85 90 95
aac gca aaa att ttt aaa ggc atg att aag agc atc atg gac agc ggc 336
Asn Ala Lys Ile Phe Lys Gly Met Ile Lys Ser Ile Met Asp Ser Gly
100 105 110
ttt aac ggg att ttt ctt gtt gcc agc aac ccg gtt gac att ttg aca 384
Phe Asn Gly Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile Leu Thr
115 120 125
tat gta act tgg aaa gag tcc ggc ctg ccg aaa gaa cat gtt atc ggt 432
Tyr Val Thr Trp Lys Glu Ser Gly Leu Pro Lys Glu His Val Ile Gly
130 135 140
tcg ggc aca gtg ctt gac tcc gcg cgt ctc cgc aac tct tta agc gcc 480
Ser Gly Thr Val Leu Asp Ser Ala Arg Leu Arg Asn Ser Leu Ser Ala
145 150 155 160
cac ttc gga att gac ccg cgc aat gtc cat gcc gca att atc ggc gaa 528
His Phe Gly Ile Asp Pro Arg Asn Val His Ala Ala Ile Ile Gly Glu
165 170 175
cac ggc gac acg gaa ctt ccg gtt tgg agc cat aca acg atc ggt tat 576
His Gly Asp Thr Glu Leu Pro Val Trp Ser His Thr Thr Ile Gly Tyr
180 185 190
gac acc att gaa agc tat ctg caa aag gga acc att gac caa aaa aca 624
Asp Thr Ile Glu Ser Tyr Leu Gln Lys Gly Thr Ile Asp Gln Lys Thr
195 200 205
tta gat gat att ttt gtc aac acg aga gat gcg gct tac cat atc att 672
Leu Asp Asp Ile Phe Val Asn Thr Arg Asp Ala Ala Tyr His Ile Ile
210 215 220
gaa cga aaa ggg gcc aca ttt tac ggc atc ggg atg tct ctg acc cgg 720
Glu Arg Lys Gly Ala Thr Phe Tyr Gly Ile Gly Met Ser Leu Thr Arg
225 230 235 240
atc aca aga gcg atc ctg aac aat gaa aac agt gtt ttg aca gtc tct 768
Ile Thr Arg Ala Ile Leu Asn Asn Glu Asn Ser Val Leu Thr Val Ser
245 250 255
gcc ttt ttg gaa ggc cag tac gga aac agc gat gtg tac att ggt gtt 816
Ala Phe Leu Glu Gly Gln Tyr Gly Asn Ser Asp Val Tyr Ile Gly Val
260 265 270
cct gcc gtt att aac cgc caa ggc gtc cgt gaa gtg gtt gaa atc gag 864
Pro Ala Val Ile Asn Arg Gln Gly Val Arg Glu Val Val Glu Ile Glu
275 280 285
ctg aac gac aaa gaa cag gaa caa ttt agc cat tct gtt aaa gta tta 912
Leu Asn Asp Lys Glu Gln Glu Gln Phe Ser His Ser Val Lys Val Leu
290 295 300
aaa gaa acg atg gca cct gta ttg taa 939
Lys Glu Thr Met Ala Pro Val Leu
305 310
<210> 34
<211> 312
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 34
Met Lys Lys Val Asn Arg Ile Ala Val Val Gly Thr Gly Ala Val Gly
1 5 10 15
Thr Ser Tyr Cys Tyr Ala Met Ile Asn Gln Gly Val Ala Glu Glu Leu
20 25 30
Val Leu Ile Asp Ile Asn Glu Ala Lys Ala Glu Gly Glu Ala Met Asp
35 40 45
Leu Asn His Gly Leu Pro Phe Ala Pro Thr Pro Thr Arg Val Trp Lys
50 55 60
Gly Asp Tyr Ser Asp Cys Gly Thr Ala Asp Leu Val Val Ile Thr Ala
65 70 75 80
Gly Ser Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp Leu Val Ala Lys
85 90 95
Asn Ala Lys Ile Phe Lys Gly Met Ile Lys Ser Ile Met Asp Ser Gly
100 105 110
Phe Asn Gly Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile Leu Thr
115 120 125
Tyr Val Thr Trp Lys Glu Ser Gly Leu Pro Lys Glu His Val Ile Gly
130 135 140
Ser Gly Thr Val Leu Asp Ser Ala Arg Leu Arg Asn Ser Leu Ser Ala
145 150 155 160
His Phe Gly Ile Asp Pro Arg Asn Val His Ala Ala Ile Ile Gly Glu
165 170 175
His Gly Asp Thr Glu Leu Pro Val Trp Ser His Thr Thr Ile Gly Tyr
180 185 190
Asp Thr Ile Glu Ser Tyr Leu Gln Lys Gly Thr Ile Asp Gln Lys Thr
195 200 205
Leu Asp Asp Ile Phe Val Asn Thr Arg Asp Ala Ala Tyr His Ile Ile
210 215 220
Glu Arg Lys Gly Ala Thr Phe Tyr Gly Ile Gly Met Ser Leu Thr Arg
225 230 235 240
Ile Thr Arg Ala Ile Leu Asn Asn Glu Asn Ser Val Leu Thr Val Ser
245 250 255
Ala Phe Leu Glu Gly Gln Tyr Gly Asn Ser Asp Val Tyr Ile Gly Val
260 265 270
Pro Ala Val Ile Asn Arg Gln Gly Val Arg Glu Val Val Glu Ile Glu
275 280 285
Leu Asn Asp Lys Glu Gln Glu Gln Phe Ser His Ser Val Lys Val Leu
290 295 300
Lys Glu Thr Met Ala Pro Val Leu
305 310
<210> 35
<211> 984
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(984)
<223> 凝结芽孢杆菌 36D1: 基因 Bcoa_3093编码乳酸脱氢酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（GTG）处的tRNA插入Met而不是Val
<400> 35
gtg aaa aag aca aaa tta gta gtt gca ggc gtt ggg cat gtc ggt tcg 48
Val Lys Lys Thr Lys Leu Val Val Ala Gly Val Gly His Val Gly Ser
1 5 10 15
tat gtg ctt gca aat gcg atg aaa ctg ggc ctt ttt tcg gaa att gct 96
Tyr Val Leu Ala Asn Ala Met Lys Leu Gly Leu Phe Ser Glu Ile Ala
20 25 30
gta ttg gat aaa aag aaa ggg gtc gca ttt ggg gaa gcg ctc gat tgg 144
Val Leu Asp Lys Lys Lys Gly Val Ala Phe Gly Glu Ala Leu Asp Trp
35 40 45
aga cat gcc acg gca ctt aca tat atg cca aac aca agc gtg aaa gca 192
Arg His Ala Thr Ala Leu Thr Tyr Met Pro Asn Thr Ser Val Lys Ala
50 55 60
ggg gat tac tcg gaa tgc gcc gat gcg gac gtc att att tgt gca gca 240
Gly Asp Tyr Ser Glu Cys Ala Asp Ala Asp Val Ile Ile Cys Ala Ala
65 70 75 80
ggg cca agc gtg ctt ccg tcg gaa aaa gac gaa atg ccg gac cgg gca 288
Gly Pro Ser Val Leu Pro Ser Glu Lys Asp Glu Met Pro Asp Arg Ala
85 90 95
ggg ctt gcc agg acg aat gcc gcc gtg gtg cgg gaa gtc atg gca ggg 336
Gly Leu Ala Arg Thr Asn Ala Ala Val Val Arg Glu Val Met Ala Gly
100 105 110
att acg aaa tat aca aaa gaa gct gtc atc att ttc att aca aat ccg 384
Ile Thr Lys Tyr Thr Lys Glu Ala Val Ile Ile Phe Ile Thr Asn Pro
115 120 125
ctt gat acg att gtc tat att gct gaa aat gaa ttt ggc tat tcg aaa 432
Leu Asp Thr Ile Val Tyr Ile Ala Glu Asn Glu Phe Gly Tyr Ser Lys
130 135 140
ggg cgg ata ttc gga aca ggc acc atg ctt gac tcg gcc cgc ctc cgc 480
Gly Arg Ile Phe Gly Thr Gly Thr Met Leu Asp Ser Ala Arg Leu Arg
145 150 155 160
cag ctt gtt gcc gaa aat tac agc att gat ccg aaa agt gta acg ggt 528
Gln Leu Val Ala Glu Asn Tyr Ser Ile Asp Pro Lys Ser Val Thr Gly
165 170 175
tat atg atg ggc gaa cac ggg ttt acc gca ttt ccg gtt ttc agt cgt 576
Tyr Met Met Gly Glu His Gly Phe Thr Ala Phe Pro Val Phe Ser Arg
180 185 190
tta aat gta cag gga ttc cgt gaa aaa gaa ctg gac agc gtg ttt aaa 624
Leu Asn Val Gln Gly Phe Arg Glu Lys Glu Leu Asp Ser Val Phe Lys
195 200 205
gga aaa gaa ccg ttg gac agg gaa gct ttc aga caa aag gtg gtg aaa 672
Gly Lys Glu Pro Leu Asp Arg Glu Ala Phe Arg Gln Lys Val Val Lys
210 215 220
act gct ttt gat gtc ctg aat ggg aaa ggg tgg acc aat gcc ggt gtt 720
Thr Ala Phe Asp Val Leu Asn Gly Lys Gly Trp Thr Asn Ala Gly Val
225 230 235 240
gct gaa gct gca gtg aca ttg gca aaa gcc gtg atg ctg gat gaa aaa 768
Ala Glu Ala Ala Val Thr Leu Ala Lys Ala Val Met Leu Asp Glu Lys
245 250 255
agc att tat ccg gtt tca gcc act ttg cat ggt cag tac gga tat aac 816
Ser Ile Tyr Pro Val Ser Ala Thr Leu His Gly Gln Tyr Gly Tyr Asn
260 265 270
ggt gat gtc gct tta agc ata cca agt gtc atc ggc cgt aac ggg atc 864
Gly Asp Val Ala Leu Ser Ile Pro Ser Val Ile Gly Arg Asn Gly Ile
275 280 285
gaa caa cag ctt gaa att gaa ctc gat gaa cag gaa aca tcc tgg ctg 912
Glu Gln Gln Leu Glu Ile Glu Leu Asp Glu Gln Glu Thr Ser Trp Leu
290 295 300
cac gaa tcg gca aaa tcg atc caa aat aca atg gca tcg gta aaa acc 960
His Glu Ser Ala Lys Ser Ile Gln Asn Thr Met Ala Ser Val Lys Thr
305 310 315 320
gga aaa tcg tat att cag gct tga 984
Gly Lys Ser Tyr Ile Gln Ala
325
<210> 36
<211> 327
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 36
Val Lys Lys Thr Lys Leu Val Val Ala Gly Val Gly His Val Gly Ser
1 5 10 15
Tyr Val Leu Ala Asn Ala Met Lys Leu Gly Leu Phe Ser Glu Ile Ala
20 25 30
Val Leu Asp Lys Lys Lys Gly Val Ala Phe Gly Glu Ala Leu Asp Trp
35 40 45
Arg His Ala Thr Ala Leu Thr Tyr Met Pro Asn Thr Ser Val Lys Ala
50 55 60
Gly Asp Tyr Ser Glu Cys Ala Asp Ala Asp Val Ile Ile Cys Ala Ala
65 70 75 80
Gly Pro Ser Val Leu Pro Ser Glu Lys Asp Glu Met Pro Asp Arg Ala
85 90 95
Gly Leu Ala Arg Thr Asn Ala Ala Val Val Arg Glu Val Met Ala Gly
100 105 110
Ile Thr Lys Tyr Thr Lys Glu Ala Val Ile Ile Phe Ile Thr Asn Pro
115 120 125
Leu Asp Thr Ile Val Tyr Ile Ala Glu Asn Glu Phe Gly Tyr Ser Lys
130 135 140
Gly Arg Ile Phe Gly Thr Gly Thr Met Leu Asp Ser Ala Arg Leu Arg
145 150 155 160
Gln Leu Val Ala Glu Asn Tyr Ser Ile Asp Pro Lys Ser Val Thr Gly
165 170 175
Tyr Met Met Gly Glu His Gly Phe Thr Ala Phe Pro Val Phe Ser Arg
180 185 190
Leu Asn Val Gln Gly Phe Arg Glu Lys Glu Leu Asp Ser Val Phe Lys
195 200 205
Gly Lys Glu Pro Leu Asp Arg Glu Ala Phe Arg Gln Lys Val Val Lys
210 215 220
Thr Ala Phe Asp Val Leu Asn Gly Lys Gly Trp Thr Asn Ala Gly Val
225 230 235 240
Ala Glu Ala Ala Val Thr Leu Ala Lys Ala Val Met Leu Asp Glu Lys
245 250 255
Ser Ile Tyr Pro Val Ser Ala Thr Leu His Gly Gln Tyr Gly Tyr Asn
260 265 270
Gly Asp Val Ala Leu Ser Ile Pro Ser Val Ile Gly Arg Asn Gly Ile
275 280 285
Glu Gln Gln Leu Glu Ile Glu Leu Asp Glu Gln Glu Thr Ser Trp Leu
290 295 300
His Glu Ser Ala Lys Ser Ile Gln Asn Thr Met Ala Ser Val Lys Thr
305 310 315 320
Gly Lys Ser Tyr Ile Gln Ala
325
<210> 37
<211> 972
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(972)
<223> 乳酸乳球菌乳脂亚种（ Lactococcus lactis subsp. cremoris） llmg_1429(ldhX) 基因编码乳酸脱氢酶
<400> 37
atg aaa att aat aat aaa aaa gtt gta att gtg gga gct gga gca gta 48
Met Lys Ile Asn Asn Lys Lys Val Val Ile Val Gly Ala Gly Ala Val
1 5 10 15
ggg agt act tat gct cat aat ttg gtc gtt gat gat tta gca gat gaa 96
Gly Ser Thr Tyr Ala His Asn Leu Val Val Asp Asp Leu Ala Asp Glu
20 25 30
atc gcc att atc aat act aat aaa agt aag gct tca gcc aat agt ttg 144
Ile Ala Ile Ile Asn Thr Asn Lys Ser Lys Ala Ser Ala Asn Ser Leu
35 40 45
gac tta ctt cat gct ctg cct tat tta aac tca gct cct aaa aat att 192
Asp Leu Leu His Ala Leu Pro Tyr Leu Asn Ser Ala Pro Lys Asn Ile
50 55 60
tat gca gca gac tac gct gat gtt gct gac gcc gat atc gtt gtt tta 240
Tyr Ala Ala Asp Tyr Ala Asp Val Ala Asp Ala Asp Ile Val Val Leu
65 70 75 80
tcc gct aat gct ccg tca gct acc ttt ggt aaa aat cca gac cgt ctt 288
Ser Ala Asn Ala Pro Ser Ala Thr Phe Gly Lys Asn Pro Asp Arg Leu
85 90 95
caa tta cta gaa aat aat gtt gag atg att cgt gat atc acg cga aaa 336
Gln Leu Leu Glu Asn Asn Val Glu Met Ile Arg Asp Ile Thr Arg Lys
100 105 110
aca atg gac gct ggc ttt gac ggt att ttc tta gta gct tca aac ccc 384
Thr Met Asp Ala Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn Pro
115 120 125
gta gac gta ctt gct caa gta gtt gct gaa gtt tct ggc tta cct aaa 432
Val Asp Val Leu Ala Gln Val Val Ala Glu Val Ser Gly Leu Pro Lys
130 135 140
cac cgg att att gga aca gga acc ttg ctt gaa aca agt cga atg cga 480
His Arg Ile Ile Gly Thr Gly Thr Leu Leu Glu Thr Ser Arg Met Arg
145 150 155 160
caa att gta gct gag aaa ctt caa att aac cct aaa agt att cat ggt 528
Gln Ile Val Ala Glu Lys Leu Gln Ile Asn Pro Lys Ser Ile His Gly
165 170 175
tat gtt tta gct gaa cat ggg aaa tct agt ttt gcc gct tgg tcc aat 576
Tyr Val Leu Ala Glu His Gly Lys Ser Ser Phe Ala Ala Trp Ser Asn
180 185 190
gtt act gtt ggg gca att cca tta aca act tgg ctt gaa aaa tat cca 624
Val Thr Val Gly Ala Ile Pro Leu Thr Thr Trp Leu Glu Lys Tyr Pro
195 200 205
aat cca gat ttt ccg aca ttt gat gaa att gat caa gaa att cgt gaa 672
Asn Pro Asp Phe Pro Thr Phe Asp Glu Ile Asp Gln Glu Ile Arg Glu
210 215 220
gtt ggt tta gat att ttc atg caa aaa gga aat act tca tac gga atc 720
Val Gly Leu Asp Ile Phe Met Gln Lys Gly Asn Thr Ser Tyr Gly Ile
225 230 235 240
gca gct tca tta gca cgt tta aca aga gca att ttc cga aat gag agc 768
Ala Ala Ser Leu Ala Arg Leu Thr Arg Ala Ile Phe Arg Asn Glu Ser
245 250 255
gtc att tta cct gtt tcg gct cat ttg aca ggt cag tat gga caa gct 816
Val Ile Leu Pro Val Ser Ala His Leu Thr Gly Gln Tyr Gly Gln Ala
260 265 270
gaa cta tat aca ggt agt cca gca ata atc gac cgg aca ggt gtt cga 864
Glu Leu Tyr Thr Gly Ser Pro Ala Ile Ile Asp Arg Thr Gly Val Arg
275 280 285
gcc gtt tta gaa ttg gaa tta aca gca gaa gag caa ggt aaa ttt gaa 912
Ala Val Leu Glu Leu Glu Leu Thr Ala Glu Glu Gln Gly Lys Phe Glu
290 295 300
cat tct gca gca ctc tta aaa gag aac ttt gaa tcc atc aag gaa aaa 960
His Ser Ala Ala Leu Leu Lys Glu Asn Phe Glu Ser Ile Lys Glu Lys
305 310 315 320
tgc aca tta taa 972
Cys Thr Leu
<210> 38
<211> 323
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 38
Met Lys Ile Asn Asn Lys Lys Val Val Ile Val Gly Ala Gly Ala Val
1 5 10 15
Gly Ser Thr Tyr Ala His Asn Leu Val Val Asp Asp Leu Ala Asp Glu
20 25 30
Ile Ala Ile Ile Asn Thr Asn Lys Ser Lys Ala Ser Ala Asn Ser Leu
35 40 45
Asp Leu Leu His Ala Leu Pro Tyr Leu Asn Ser Ala Pro Lys Asn Ile
50 55 60
Tyr Ala Ala Asp Tyr Ala Asp Val Ala Asp Ala Asp Ile Val Val Leu
65 70 75 80
Ser Ala Asn Ala Pro Ser Ala Thr Phe Gly Lys Asn Pro Asp Arg Leu
85 90 95
Gln Leu Leu Glu Asn Asn Val Glu Met Ile Arg Asp Ile Thr Arg Lys
100 105 110
Thr Met Asp Ala Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn Pro
115 120 125
Val Asp Val Leu Ala Gln Val Val Ala Glu Val Ser Gly Leu Pro Lys
130 135 140
His Arg Ile Ile Gly Thr Gly Thr Leu Leu Glu Thr Ser Arg Met Arg
145 150 155 160
Gln Ile Val Ala Glu Lys Leu Gln Ile Asn Pro Lys Ser Ile His Gly
165 170 175
Tyr Val Leu Ala Glu His Gly Lys Ser Ser Phe Ala Ala Trp Ser Asn
180 185 190
Val Thr Val Gly Ala Ile Pro Leu Thr Thr Trp Leu Glu Lys Tyr Pro
195 200 205
Asn Pro Asp Phe Pro Thr Phe Asp Glu Ile Asp Gln Glu Ile Arg Glu
210 215 220
Val Gly Leu Asp Ile Phe Met Gln Lys Gly Asn Thr Ser Tyr Gly Ile
225 230 235 240
Ala Ala Ser Leu Ala Arg Leu Thr Arg Ala Ile Phe Arg Asn Glu Ser
245 250 255
Val Ile Leu Pro Val Ser Ala His Leu Thr Gly Gln Tyr Gly Gln Ala
260 265 270
Glu Leu Tyr Thr Gly Ser Pro Ala Ile Ile Asp Arg Thr Gly Val Arg
275 280 285
Ala Val Leu Glu Leu Glu Leu Thr Ala Glu Glu Gln Gly Lys Phe Glu
290 295 300
His Ser Ala Ala Leu Leu Lys Glu Asn Phe Glu Ser Ile Lys Glu Lys
305 310 315 320
Cys Thr Leu
<210> 39
<211> 945
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(945)
<223> 乳酸乳球菌乳脂亚种 llmg_0392 (ldhB)基因编码乳酸脱氢酶
<400> 39
atg aaa att aca agt aga aaa gta gtc gtg att gga aca gga ttt gtc 48
Met Lys Ile Thr Ser Arg Lys Val Val Val Ile Gly Thr Gly Phe Val
1 5 10 15
gga aca agt att gct tat tca atg att aat caa ggg ctt gtc aac gag 96
Gly Thr Ser Ile Ala Tyr Ser Met Ile Asn Gln Gly Leu Val Asn Glu
20 25 30
tta gtt ttg ata gat gtc aat caa gat aaa gct gag ggt gag gct tta 144
Leu Val Leu Ile Asp Val Asn Gln Asp Lys Ala Glu Gly Glu Ala Leu
35 40 45
gat tta ttg gat ggc ata tct tgg gct caa gaa aat gtt att gta aga 192
Asp Leu Leu Asp Gly Ile Ser Trp Ala Gln Glu Asn Val Ile Val Arg
50 55 60
gct gga aat tac aag gat tgt gaa aat gca gat att gtc gtg att aca 240
Ala Gly Asn Tyr Lys Asp Cys Glu Asn Ala Asp Ile Val Val Ile Thr
65 70 75 80
gct ggt gtg aac caa aaa ccc gga caa tca cgt tta gat ttg gtc aat 288
Ala Gly Val Asn Gln Lys Pro Gly Gln Ser Arg Leu Asp Leu Val Asn
85 90 95
aca aat gct aaa att atg cgc tca atc gtt act caa gtt atg gat tca 336
Thr Asn Ala Lys Ile Met Arg Ser Ile Val Thr Gln Val Met Asp Ser
100 105 110
ggt ttt gat ggt att ttt gta att gct tct aat cct gtg gat atc ctt 384
Gly Phe Asp Gly Ile Phe Val Ile Ala Ser Asn Pro Val Asp Ile Leu
115 120 125
acc tat gtc gct tgg gaa act tct ggt ctt gac caa tct aga att gtc 432
Thr Tyr Val Ala Trp Glu Thr Ser Gly Leu Asp Gln Ser Arg Ile Val
130 135 140
gga act ggt aca act ttg gat acc aca cgt ttt aga aaa gaa ttg gcc 480
Gly Thr Gly Thr Thr Leu Asp Thr Thr Arg Phe Arg Lys Glu Leu Ala
145 150 155 160
aca aaa cta gaa att gac cct cgg agt gtt cac gga tat att att ggt 528
Thr Lys Leu Glu Ile Asp Pro Arg Ser Val His Gly Tyr Ile Ile Gly
165 170 175
gag cat ggc gat tct gaa gtt gca gtt tgg tca cac act acg ata ggt 576
Glu His Gly Asp Ser Glu Val Ala Val Trp Ser His Thr Thr Ile Gly
180 185 190
gga aaa cct att ctt gaa ttt att gtt aaa aat aaa aaa att ggt ctt 624
Gly Lys Pro Ile Leu Glu Phe Ile Val Lys Asn Lys Lys Ile Gly Leu
195 200 205
gaa gat ttg tct aat ttg tca aat aag gtt aaa aat gct gct tat gaa 672
Glu Asp Leu Ser Asn Leu Ser Asn Lys Val Lys Asn Ala Ala Tyr Glu
210 215 220
att att gat aaa aaa caa gcc act tat tac gga att gga atg agc aca 720
Ile Ile Asp Lys Lys Gln Ala Thr Tyr Tyr Gly Ile Gly Met Ser Thr
225 230 235 240
gct aga att gtc aaa gcc ata tta aat aat gaa caa gtc att cta cca 768
Ala Arg Ile Val Lys Ala Ile Leu Asn Asn Glu Gln Val Ile Leu Pro
245 250 255
gtt tct gcc tat ttg cgt gga gaa tat ggt caa gaa ggg gtc ttt act 816
Val Ser Ala Tyr Leu Arg Gly Glu Tyr Gly Gln Glu Gly Val Phe Thr
260 265 270
ggt gtt cca tct gtc gta aat caa aat ggt gtc aga gaa att att gaa 864
Gly Val Pro Ser Val Val Asn Gln Asn Gly Val Arg Glu Ile Ile Glu
275 280 285
ctc aat att gat gct tat gaa atg aaa caa ttt gaa aag tct gtc agt 912
Leu Asn Ile Asp Ala Tyr Glu Met Lys Gln Phe Glu Lys Ser Val Ser
290 295 300
caa ctt aaa gaa gtc ata gaa tct att aaa taa 945
Gln Leu Lys Glu Val Ile Glu Ser Ile Lys
305 310
<210> 40
<211> 314
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 40
Met Lys Ile Thr Ser Arg Lys Val Val Val Ile Gly Thr Gly Phe Val
1 5 10 15
Gly Thr Ser Ile Ala Tyr Ser Met Ile Asn Gln Gly Leu Val Asn Glu
20 25 30
Leu Val Leu Ile Asp Val Asn Gln Asp Lys Ala Glu Gly Glu Ala Leu
35 40 45
Asp Leu Leu Asp Gly Ile Ser Trp Ala Gln Glu Asn Val Ile Val Arg
50 55 60
Ala Gly Asn Tyr Lys Asp Cys Glu Asn Ala Asp Ile Val Val Ile Thr
65 70 75 80
Ala Gly Val Asn Gln Lys Pro Gly Gln Ser Arg Leu Asp Leu Val Asn
85 90 95
Thr Asn Ala Lys Ile Met Arg Ser Ile Val Thr Gln Val Met Asp Ser
100 105 110
Gly Phe Asp Gly Ile Phe Val Ile Ala Ser Asn Pro Val Asp Ile Leu
115 120 125
Thr Tyr Val Ala Trp Glu Thr Ser Gly Leu Asp Gln Ser Arg Ile Val
130 135 140
Gly Thr Gly Thr Thr Leu Asp Thr Thr Arg Phe Arg Lys Glu Leu Ala
145 150 155 160
Thr Lys Leu Glu Ile Asp Pro Arg Ser Val His Gly Tyr Ile Ile Gly
165 170 175
Glu His Gly Asp Ser Glu Val Ala Val Trp Ser His Thr Thr Ile Gly
180 185 190
Gly Lys Pro Ile Leu Glu Phe Ile Val Lys Asn Lys Lys Ile Gly Leu
195 200 205
Glu Asp Leu Ser Asn Leu Ser Asn Lys Val Lys Asn Ala Ala Tyr Glu
210 215 220
Ile Ile Asp Lys Lys Gln Ala Thr Tyr Tyr Gly Ile Gly Met Ser Thr
225 230 235 240
Ala Arg Ile Val Lys Ala Ile Leu Asn Asn Glu Gln Val Ile Leu Pro
245 250 255
Val Ser Ala Tyr Leu Arg Gly Glu Tyr Gly Gln Glu Gly Val Phe Thr
260 265 270
Gly Val Pro Ser Val Val Asn Gln Asn Gly Val Arg Glu Ile Ile Glu
275 280 285
Leu Asn Ile Asp Ala Tyr Glu Met Lys Gln Phe Glu Lys Ser Val Ser
290 295 300
Gln Leu Lys Glu Val Ile Glu Ser Ile Lys
305 310
<210> 41
<211> 981
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(981)
<223> 乳酸乳球菌乳脂亚种 MG1363: 基因 llmg_0763
编码磷酸转乙酰酶
<400> 41
atg gaa ctt ttt gaa tca ctg aaa caa aag att agt gga aaa gac tta 48
Met Glu Leu Phe Glu Ser Leu Lys Gln Lys Ile Ser Gly Lys Asp Leu
1 5 10 15
aaa att gtc ttt cct gaa gga act gat aca cgt att tta ggt gct gca 96
Lys Ile Val Phe Pro Glu Gly Thr Asp Thr Arg Ile Leu Gly Ala Ala
20 25 30
aat cgt ctt aat gcc gat gga ttg att aca cct gtc ttt att gga aat 144
Asn Arg Leu Asn Ala Asp Gly Leu Ile Thr Pro Val Phe Ile Gly Asn
35 40 45
gtc aaa gaa gtc act gaa aca ttg att tca cgt gga att aac cca gag 192
Val Lys Glu Val Thr Glu Thr Leu Ile Ser Arg Gly Ile Asn Pro Glu
50 55 60
ggc ttt gag att tac gac cca gaa aat tgt gga cgt ttt gaa aca atg 240
Gly Phe Glu Ile Tyr Asp Pro Glu Asn Cys Gly Arg Phe Glu Thr Met
65 70 75 80
aca gaa aca ttt gtt gaa cgt cgc aag ggt aaa gtt aca gaa gaa caa 288
Thr Glu Thr Phe Val Glu Arg Arg Lys Gly Lys Val Thr Glu Glu Gln
85 90 95
gca aaa gaa att ttg aaa gac cca aat tac ttt gga aca atg ctt gtt 336
Ala Lys Glu Ile Leu Lys Asp Pro Asn Tyr Phe Gly Thr Met Leu Val
100 105 110
tat atg gga att gct gac gga atg gtt tct ggt gca att cat tca act 384
Tyr Met Gly Ile Ala Asp Gly Met Val Ser Gly Ala Ile His Ser Thr
115 120 125
gct gat act gtt cgt ccg gct ctc caa atc att aaa aca aaa cca gga 432
Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Pro Gly
130 135 140
gta aaa tct gtt tct ggt gcc ttt att atg gtt cgc ggt cgt gat gac 480
Val Lys Ser Val Ser Gly Ala Phe Ile Met Val Arg Gly Arg Asp Asp
145 150 155 160
aat gaa aaa tat att ttt ggt gac tgt gca att aac atc aaa cct gat 528
Asn Glu Lys Tyr Ile Phe Gly Asp Cys Ala Ile Asn Ile Lys Pro Asp
165 170 175
gct cca aca ctt gct gat atc gcc gta gcc tca gcc gaa act gca aga 576
Ala Pro Thr Leu Ala Asp Ile Ala Val Ala Ser Ala Glu Thr Ala Arg
180 185 190
ctc ttt gat att gac cct aaa att gcg atg ctt tca ttc tca act aaa 624
Leu Phe Asp Ile Asp Pro Lys Ile Ala Met Leu Ser Phe Ser Thr Lys
195 200 205
ggt tct ggt aaa tca gaa gat gtg gat aaa gtg gtt gaa gca aca aca 672
Gly Ser Gly Lys Ser Glu Asp Val Asp Lys Val Val Glu Ala Thr Thr
210 215 220
cta gtt aaa gaa ggt cac cct gaa gtt gaa att gat ggg gaa ctt caa 720
Leu Val Lys Glu Gly His Pro Glu Val Glu Ile Asp Gly Glu Leu Gln
225 230 235 240
ttt gat gct gcc ttt gtt cca gct gtt gca cgt caa aaa gca cct gat 768
Phe Asp Ala Ala Phe Val Pro Ala Val Ala Arg Gln Lys Ala Pro Asp
245 250 255
tca gaa gtt gct ggt cgt gca aac gtc ttt atc ttc cca gac att caa 816
Ser Glu Val Ala Gly Arg Ala Asn Val Phe Ile Phe Pro Asp Ile Gln
260 265 270
tca ggg aat att gga tac aaa att gcg caa cgt cta ggt aat ttt gaa 864
Ser Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Asn Phe Glu
275 280 285
gcc att ggc cca atc tta caa gga ttg aat gcg cct gtt tct gac ctt 912
Ala Ile Gly Pro Ile Leu Gln Gly Leu Asn Ala Pro Val Ser Asp Leu
290 295 300
tca cgc ggt tgt aac gaa gaa gat gtt tat aaa ctt gca atc atc aca 960
Ser Arg Gly Cys Asn Glu Glu Asp Val Tyr Lys Leu Ala Ile Ile Thr
305 310 315 320
gct gct caa gct tta aaa taa 981
Ala Ala Gln Ala Leu Lys
325
<210> 42
<211> 326
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 42
Met Glu Leu Phe Glu Ser Leu Lys Gln Lys Ile Ser Gly Lys Asp Leu
1 5 10 15
Lys Ile Val Phe Pro Glu Gly Thr Asp Thr Arg Ile Leu Gly Ala Ala
20 25 30
Asn Arg Leu Asn Ala Asp Gly Leu Ile Thr Pro Val Phe Ile Gly Asn
35 40 45
Val Lys Glu Val Thr Glu Thr Leu Ile Ser Arg Gly Ile Asn Pro Glu
50 55 60
Gly Phe Glu Ile Tyr Asp Pro Glu Asn Cys Gly Arg Phe Glu Thr Met
65 70 75 80
Thr Glu Thr Phe Val Glu Arg Arg Lys Gly Lys Val Thr Glu Glu Gln
85 90 95
Ala Lys Glu Ile Leu Lys Asp Pro Asn Tyr Phe Gly Thr Met Leu Val
100 105 110
Tyr Met Gly Ile Ala Asp Gly Met Val Ser Gly Ala Ile His Ser Thr
115 120 125
Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Pro Gly
130 135 140
Val Lys Ser Val Ser Gly Ala Phe Ile Met Val Arg Gly Arg Asp Asp
145 150 155 160
Asn Glu Lys Tyr Ile Phe Gly Asp Cys Ala Ile Asn Ile Lys Pro Asp
165 170 175
Ala Pro Thr Leu Ala Asp Ile Ala Val Ala Ser Ala Glu Thr Ala Arg
180 185 190
Leu Phe Asp Ile Asp Pro Lys Ile Ala Met Leu Ser Phe Ser Thr Lys
195 200 205
Gly Ser Gly Lys Ser Glu Asp Val Asp Lys Val Val Glu Ala Thr Thr
210 215 220
Leu Val Lys Glu Gly His Pro Glu Val Glu Ile Asp Gly Glu Leu Gln
225 230 235 240
Phe Asp Ala Ala Phe Val Pro Ala Val Ala Arg Gln Lys Ala Pro Asp
245 250 255
Ser Glu Val Ala Gly Arg Ala Asn Val Phe Ile Phe Pro Asp Ile Gln
260 265 270
Ser Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Asn Phe Glu
275 280 285
Ala Ile Gly Pro Ile Leu Gln Gly Leu Asn Ala Pro Val Ser Asp Leu
290 295 300
Ser Arg Gly Cys Asn Glu Glu Asp Val Tyr Lys Leu Ala Ile Ile Thr
305 310 315 320
Ala Ala Gln Ala Leu Lys
325
<210> 43
<211> 990
<212> DNA
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<220>
<221> CDS
<222> (1)..(990)
<223> 嗜酸乳杆菌 NCFM: 基因 LBA0704 编码磷酸转乙酰酶
<400> 43
atg agc gta ttt tca tta ttc aaa gaa caa gtt gaa caa gct gat gaa 48
Met Ser Val Phe Ser Leu Phe Lys Glu Gln Val Glu Gln Ala Asp Glu
1 5 10 15
aag aaa aaa att att ttc cca gaa agt aat gat ctt aga atc tta act 96
Lys Lys Lys Ile Ile Phe Pro Glu Ser Asn Asp Leu Arg Ile Leu Thr
20 25 30
gca gtt tcc aat tta aat aag gac aat att atc gat cca att ttg att 144
Ala Val Ser Asn Leu Asn Lys Asp Asn Ile Ile Asp Pro Ile Leu Ile
35 40 45
ggt aaa aga gaa gat att gaa aaa ttg gct gca gaa aat aat tta gat 192
Gly Lys Arg Glu Asp Ile Glu Lys Leu Ala Ala Glu Asn Asn Leu Asp
50 55 60
ctt gat ggt gta aag att tat gat caa aat aat tat gct ggt tta gat 240
Leu Asp Gly Val Lys Ile Tyr Asp Gln Asn Asn Tyr Ala Gly Leu Asp
65 70 75 80
gaa atg gct gaa gca ttt gtt aaa gct aga aaa aag gat act agt att 288
Glu Met Ala Glu Ala Phe Val Lys Ala Arg Lys Lys Asp Thr Ser Ile
85 90 95
gcc gaa gca aag gct aag cta gca aaa ggt aat tac ttt ggt act atg 336
Ala Glu Ala Lys Ala Lys Leu Ala Lys Gly Asn Tyr Phe Gly Thr Met
100 105 110
cta gta aag atg gga aaa gct gat gga atg gta tca ggt gct gcg cat 384
Leu Val Lys Met Gly Lys Ala Asp Gly Met Val Ser Gly Ala Ala His
115 120 125
tca act gct aat act gtt tta cct gct tta caa tta att cat gct gct 432
Ser Thr Ala Asn Thr Val Leu Pro Ala Leu Gln Leu Ile His Ala Ala
130 135 140
aag ggg atg cat cgc gta tcg ggt gct ttc gta atg gaa aaa ggc gat 480
Lys Gly Met His Arg Val Ser Gly Ala Phe Val Met Glu Lys Gly Asp
145 150 155 160
gaa aga tat att ttt gct gat tgt gct att aat att gat cca gat gaa 528
Glu Arg Tyr Ile Phe Ala Asp Cys Ala Ile Asn Ile Asp Pro Asp Glu
165 170 175
gaa act ttg gca gaa att ggt tat caa tcc gct caa act gct aaa atg 576
Glu Thr Leu Ala Glu Ile Gly Tyr Gln Ser Ala Gln Thr Ala Lys Met
180 185 190
gca gga atc gat cct aag gtt gca ttt tta agc ttt tca act aaa ggg 624
Ala Gly Ile Asp Pro Lys Val Ala Phe Leu Ser Phe Ser Thr Lys Gly
195 200 205
tca gct gaa ggt cct atg gta aat aaa gtt cat gac gct gct gca atg 672
Ser Ala Glu Gly Pro Met Val Asn Lys Val His Asp Ala Ala Ala Met
210 215 220
ttc caa aaa gat cat cca gaa atc cct gca gat ggt gaa tta caa ttt 720
Phe Gln Lys Asp His Pro Glu Ile Pro Ala Asp Gly Glu Leu Gln Phe
225 230 235 240
gat gct gca ttt gtt cca tca gtt ggt gaa aaa aaa gca cca ggc tct 768
Asp Ala Ala Phe Val Pro Ser Val Gly Glu Lys Lys Ala Pro Gly Ser
245 250 255
aag gtg gca gga cat gct aat gta ttt gta ttc cca gaa ctt caa tct 816
Lys Val Ala Gly His Ala Asn Val Phe Val Phe Pro Glu Leu Gln Ser
260 265 270
gga aat atc gct tat aag atg gtg caa cga ctt ggt aac ttt act gca 864
Gly Asn Ile Ala Tyr Lys Met Val Gln Arg Leu Gly Asn Phe Thr Ala
275 280 285
gta ggt cct att tta caa ggt cta gct gct cca gtt aat gac tta tca 912
Val Gly Pro Ile Leu Gln Gly Leu Ala Ala Pro Val Asn Asp Leu Ser
290 295 300
cgt ggt tgt agt gaa caa gat att tac gat ttg gct atc gtt act gct 960
Arg Gly Cys Ser Glu Gln Asp Ile Tyr Asp Leu Ala Ile Val Thr Ala
305 310 315 320
gca caa gct ttg gca aag gat gaa aag taa 990
Ala Gln Ala Leu Ala Lys Asp Glu Lys
325
<210> 44
<211> 329
<212> PRT
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<400> 44
Met Ser Val Phe Ser Leu Phe Lys Glu Gln Val Glu Gln Ala Asp Glu
1 5 10 15
Lys Lys Lys Ile Ile Phe Pro Glu Ser Asn Asp Leu Arg Ile Leu Thr
20 25 30
Ala Val Ser Asn Leu Asn Lys Asp Asn Ile Ile Asp Pro Ile Leu Ile
35 40 45
Gly Lys Arg Glu Asp Ile Glu Lys Leu Ala Ala Glu Asn Asn Leu Asp
50 55 60
Leu Asp Gly Val Lys Ile Tyr Asp Gln Asn Asn Tyr Ala Gly Leu Asp
65 70 75 80
Glu Met Ala Glu Ala Phe Val Lys Ala Arg Lys Lys Asp Thr Ser Ile
85 90 95
Ala Glu Ala Lys Ala Lys Leu Ala Lys Gly Asn Tyr Phe Gly Thr Met
100 105 110
Leu Val Lys Met Gly Lys Ala Asp Gly Met Val Ser Gly Ala Ala His
115 120 125
Ser Thr Ala Asn Thr Val Leu Pro Ala Leu Gln Leu Ile His Ala Ala
130 135 140
Lys Gly Met His Arg Val Ser Gly Ala Phe Val Met Glu Lys Gly Asp
145 150 155 160
Glu Arg Tyr Ile Phe Ala Asp Cys Ala Ile Asn Ile Asp Pro Asp Glu
165 170 175
Glu Thr Leu Ala Glu Ile Gly Tyr Gln Ser Ala Gln Thr Ala Lys Met
180 185 190
Ala Gly Ile Asp Pro Lys Val Ala Phe Leu Ser Phe Ser Thr Lys Gly
195 200 205
Ser Ala Glu Gly Pro Met Val Asn Lys Val His Asp Ala Ala Ala Met
210 215 220
Phe Gln Lys Asp His Pro Glu Ile Pro Ala Asp Gly Glu Leu Gln Phe
225 230 235 240
Asp Ala Ala Phe Val Pro Ser Val Gly Glu Lys Lys Ala Pro Gly Ser
245 250 255
Lys Val Ala Gly His Ala Asn Val Phe Val Phe Pro Glu Leu Gln Ser
260 265 270
Gly Asn Ile Ala Tyr Lys Met Val Gln Arg Leu Gly Asn Phe Thr Ala
275 280 285
Val Gly Pro Ile Leu Gln Gly Leu Ala Ala Pro Val Asn Asp Leu Ser
290 295 300
Arg Gly Cys Ser Glu Gln Asp Ile Tyr Asp Leu Ala Ile Val Thr Ala
305 310 315 320
Ala Gln Ala Leu Ala Lys Asp Glu Lys
325
<210> 45
<211> 990
<212> DNA
<213> 德氏乳杆菌（Lactobacillus delbrueckii）
<220>
<221> CDS
<222> (1)..(990)
<223> 德氏乳杆菌保加利亚亚种 ATCC 11842 ; 基因
Ldb0643 编码磷酸转乙酰酶
<400> 45
atg gaa gtt ttt gaa caa tta aag caa ttg att aag caa aaa gac aag 48
Met Glu Val Phe Glu Gln Leu Lys Gln Leu Ile Lys Gln Lys Asp Lys
1 5 10 15
cag cgg atc gtt ttc ccg gaa ggg gag gac ccg cgg atc ctg acg gcg 96
Gln Arg Ile Val Phe Pro Glu Gly Glu Asp Pro Arg Ile Leu Thr Ala
20 25 30
gtc agc cgc tta aag caa gat gac ctg gtt gaa ccg atc gtc ctg ggg 144
Val Ser Arg Leu Lys Gln Asp Asp Leu Val Glu Pro Ile Val Leu Gly
35 40 45
caa aag gac cag gtc ctg gct ttg gcg gaa aaa gct ggc ttg gac atg 192
Gln Lys Asp Gln Val Leu Ala Leu Ala Glu Lys Ala Gly Leu Asp Met
50 55 60
acc ggg atc gaa atc gtg gac tac caa aac agc ccg gac ttt ggc gag 240
Thr Gly Ile Glu Ile Val Asp Tyr Gln Asn Ser Pro Asp Phe Gly Glu
65 70 75 80
atg gtg gaa aag ttc cac gaa cgg atg gcc ggg ggc aag cac ttc cag 288
Met Val Glu Lys Phe His Glu Arg Met Ala Gly Gly Lys His Phe Gln
85 90 95
acc atg gaa gaa gcg gaa gct gcc ttg cgc gac gcc aac ttc tac ggg 336
Thr Met Glu Glu Ala Glu Ala Ala Leu Arg Asp Ala Asn Phe Tyr Gly
100 105 110
acc atg ctg gtt tac acc ggc cag gcc gac ggg atg gtg tcc ggg gcg 384
Thr Met Leu Val Tyr Thr Gly Gln Ala Asp Gly Met Val Ser Gly Ala
115 120 125
gct cac tca acg gcc gac acg gtc cgg cca gcc ctc ttc att ttg aag 432
Ala His Ser Thr Ala Asp Thr Val Arg Pro Ala Leu Phe Ile Leu Lys
130 135 140
acc aag ccg ggg atg cac cgg att tcc ggt tcc ttc atc atg gaa aga 480
Thr Lys Pro Gly Met His Arg Ile Ser Gly Ser Phe Ile Met Glu Arg
145 150 155 160
ggg gaa gaa aag tat gtc ttt gct gac tgc gcg att tcc atc gac ccg 528
Gly Glu Glu Lys Tyr Val Phe Ala Asp Cys Ala Ile Ser Ile Asp Pro
165 170 175
acc agc gac cag ctg gtt gaa ttc gcg gcc cag tca gct gct acg gcc 576
Thr Ser Asp Gln Leu Val Glu Phe Ala Ala Gln Ser Ala Ala Thr Ala
180 185 190
aag atg atc ggg att gac ccc aag gtg gcc ttc ctc agc ttc tca acc 624
Lys Met Ile Gly Ile Asp Pro Lys Val Ala Phe Leu Ser Phe Ser Thr
195 200 205
cac ggc tca gcc aag ggc ccg cag gta gac aag gtg gca gaa gcg gcc 672
His Gly Ser Ala Lys Gly Pro Gln Val Asp Lys Val Ala Glu Ala Ala
210 215 220
aag ctg ttc aag gaa aag tac ccg gac atc ccg gca gac ggc gat ttg 720
Lys Leu Phe Lys Glu Lys Tyr Pro Asp Ile Pro Ala Asp Gly Asp Leu
225 230 235 240
cag ttt gac gcg gcc ttc gtg ccg gca gtt gct gaa ctt aag gca cct 768
Gln Phe Asp Ala Ala Phe Val Pro Ala Val Ala Glu Leu Lys Ala Pro
245 250 255
ggt tca gcc gtt ggc ggc agg gct aat gtc ttt atc ttc ccg gaa ctg 816
Gly Ser Ala Val Gly Gly Arg Ala Asn Val Phe Ile Phe Pro Glu Leu
260 265 270
cag tca ggc aac att gcc tac aag att acc cag cgc ctg ggc aac ttt 864
Gln Ser Gly Asn Ile Ala Tyr Lys Ile Thr Gln Arg Leu Gly Asn Phe
275 280 285
acg gcc atc ggg ccg gtt ttg cag ggg att gcc aag ccg gtc aac gac 912
Thr Ala Ile Gly Pro Val Leu Gln Gly Ile Ala Lys Pro Val Asn Asp
290 295 300
ctc tcc cgg ggc tgc agc gct gat gac gtc tac aag atc ggg att ttg 960
Leu Ser Arg Gly Cys Ser Ala Asp Asp Val Tyr Lys Ile Gly Ile Leu
305 310 315 320
acg gcg gct caa gcc gtt atg gaa ggg tag 990
Thr Ala Ala Gln Ala Val Met Glu Gly
325
<210> 46
<211> 329
<212> PRT
<213> 德氏乳杆菌（Lactobacillus delbrueckii）
<400> 46
Met Glu Val Phe Glu Gln Leu Lys Gln Leu Ile Lys Gln Lys Asp Lys
1 5 10 15
Gln Arg Ile Val Phe Pro Glu Gly Glu Asp Pro Arg Ile Leu Thr Ala
20 25 30
Val Ser Arg Leu Lys Gln Asp Asp Leu Val Glu Pro Ile Val Leu Gly
35 40 45
Gln Lys Asp Gln Val Leu Ala Leu Ala Glu Lys Ala Gly Leu Asp Met
50 55 60
Thr Gly Ile Glu Ile Val Asp Tyr Gln Asn Ser Pro Asp Phe Gly Glu
65 70 75 80
Met Val Glu Lys Phe His Glu Arg Met Ala Gly Gly Lys His Phe Gln
85 90 95
Thr Met Glu Glu Ala Glu Ala Ala Leu Arg Asp Ala Asn Phe Tyr Gly
100 105 110
Thr Met Leu Val Tyr Thr Gly Gln Ala Asp Gly Met Val Ser Gly Ala
115 120 125
Ala His Ser Thr Ala Asp Thr Val Arg Pro Ala Leu Phe Ile Leu Lys
130 135 140
Thr Lys Pro Gly Met His Arg Ile Ser Gly Ser Phe Ile Met Glu Arg
145 150 155 160
Gly Glu Glu Lys Tyr Val Phe Ala Asp Cys Ala Ile Ser Ile Asp Pro
165 170 175
Thr Ser Asp Gln Leu Val Glu Phe Ala Ala Gln Ser Ala Ala Thr Ala
180 185 190
Lys Met Ile Gly Ile Asp Pro Lys Val Ala Phe Leu Ser Phe Ser Thr
195 200 205
His Gly Ser Ala Lys Gly Pro Gln Val Asp Lys Val Ala Glu Ala Ala
210 215 220
Lys Leu Phe Lys Glu Lys Tyr Pro Asp Ile Pro Ala Asp Gly Asp Leu
225 230 235 240
Gln Phe Asp Ala Ala Phe Val Pro Ala Val Ala Glu Leu Lys Ala Pro
245 250 255
Gly Ser Ala Val Gly Gly Arg Ala Asn Val Phe Ile Phe Pro Glu Leu
260 265 270
Gln Ser Gly Asn Ile Ala Tyr Lys Ile Thr Gln Arg Leu Gly Asn Phe
275 280 285
Thr Ala Ile Gly Pro Val Leu Gln Gly Ile Ala Lys Pro Val Asn Asp
290 295 300
Leu Ser Arg Gly Cys Ser Ala Asp Asp Val Tyr Lys Ile Gly Ile Leu
305 310 315 320
Thr Ala Ala Gln Ala Val Met Glu Gly
325
<210> 47
<211> 978
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(978)
<223> 干酪乳杆菌 ATCC 334; 基因 LSEI_0996 编码磷酸转乙酰酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（TTG）处的tRNA插入Met而不是Leu
<400> 47
ttg gac cta ttt gaa agt tta aaa gac aag att aat ggc aaa aat tta 48
Leu Asp Leu Phe Glu Ser Leu Lys Asp Lys Ile Asn Gly Lys Asn Leu
1 5 10 15
cgg att gta ttt ccg gaa ggc gaa gat ccg cgc gtt ttg ggc gca gca 96
Arg Ile Val Phe Pro Glu Gly Glu Asp Pro Arg Val Leu Gly Ala Ala
20 25 30
gtt cgg ctc gca gct gac ggg ttg ata cag gcc atc gtg cta ggt aat 144
Val Arg Leu Ala Ala Asp Gly Leu Ile Gln Ala Ile Val Leu Gly Asn
35 40 45
cct gat aag att cag aca ttg gca gct gct aaa agc tgg gat ctc agc 192
Pro Asp Lys Ile Gln Thr Leu Ala Ala Ala Lys Ser Trp Asp Leu Ser
50 55 60
aag ttg aca gtt cgc gat cca gcc aac gat gac ctt cac gct aaa atg 240
Lys Leu Thr Val Arg Asp Pro Ala Asn Asp Asp Leu His Ala Lys Met
65 70 75 80
gtt gcg gcc ttt gtt gag cgt cgc aaa ggc aag gca acc cca gaa cag 288
Val Ala Ala Phe Val Glu Arg Arg Lys Gly Lys Ala Thr Pro Glu Gln
85 90 95
gcc gaa aaa att gtt cag gat gcc aat tat ttt ggt acg atg ctg gtt 336
Ala Glu Lys Ile Val Gln Asp Ala Asn Tyr Phe Gly Thr Met Leu Val
100 105 110
tac atg aag gaa gca gac ggt atg gtt tca ggc gca gtt cat tca acg 384
Tyr Met Lys Glu Ala Asp Gly Met Val Ser Gly Ala Val His Ser Thr
115 120 125
gct gac aca gtc cgg cct gct ttg caa att atc aag acg cgt cct ggc 432
Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Arg Pro Gly
130 135 140
gtg cat ttg gtc agt ggt gcg ttc att atg cag cgt ggc cgt gac gaa 480
Val His Leu Val Ser Gly Ala Phe Ile Met Gln Arg Gly Arg Asp Glu
145 150 155 160
cgc tac tta ttc gcg gat aac gcc atc aac att gat cca gat gct gat 528
Arg Tyr Leu Phe Ala Asp Asn Ala Ile Asn Ile Asp Pro Asp Ala Asp
165 170 175
cag ctg gct gaa att gcg gtt gag tca gct aaa act gct gcc ttg ttt 576
Gln Leu Ala Glu Ile Ala Val Glu Ser Ala Lys Thr Ala Ala Leu Phe
180 185 190
gat atc gaa cca ccg cgc gtc gcc ttg ctg tca ttc tca acc aaa ggc 624
Asp Ile Glu Pro Pro Arg Val Ala Leu Leu Ser Phe Ser Thr Lys Gly
195 200 205
agt gct aag gga ccg caa gta gat aag gta gtt gaa gcc act aag aaa 672
Ser Ala Lys Gly Pro Gln Val Asp Lys Val Val Glu Ala Thr Lys Lys
210 215 220
gct cat gaa ctg gca cct gat ttg gcc tta gac ggt gag tta caa ttt 720
Ala His Glu Leu Ala Pro Asp Leu Ala Leu Asp Gly Glu Leu Gln Phe
225 230 235 240
gat gct gct ttt gtc cca acc gtt gcc aag caa aaa gcc cct gat tcc 768
Asp Ala Ala Phe Val Pro Thr Val Ala Lys Gln Lys Ala Pro Asp Ser
245 250 255
aaa gtg gct ggg gaa gcg aat gtt ttc att ttc cct gaa tta caa tcc 816
Lys Val Ala Gly Glu Ala Asn Val Phe Ile Phe Pro Glu Leu Gln Ser
260 265 270
ggt aat att ggt tat aag att gcg caa cga ttc ggc ggg ttt gaa gcc 864
Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Phe Gly Gly Phe Glu Ala
275 280 285
att ggc cca att ttg caa ggt ttg aat cag cca gtt tct gat ttg agt 912
Ile Gly Pro Ile Leu Gln Gly Leu Asn Gln Pro Val Ser Asp Leu Ser
290 295 300
cgt ggg gca aat gaa gag gat gtc tat aag ttg gcc att att aca gcg 960
Arg Gly Ala Asn Glu Glu Asp Val Tyr Lys Leu Ala Ile Ile Thr Ala
305 310 315 320
gcc cag acc ttg cta tga 978
Ala Gln Thr Leu Leu
325
<210> 48
<211> 325
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 48
Leu Asp Leu Phe Glu Ser Leu Lys Asp Lys Ile Asn Gly Lys Asn Leu
1 5 10 15
Arg Ile Val Phe Pro Glu Gly Glu Asp Pro Arg Val Leu Gly Ala Ala
20 25 30
Val Arg Leu Ala Ala Asp Gly Leu Ile Gln Ala Ile Val Leu Gly Asn
35 40 45
Pro Asp Lys Ile Gln Thr Leu Ala Ala Ala Lys Ser Trp Asp Leu Ser
50 55 60
Lys Leu Thr Val Arg Asp Pro Ala Asn Asp Asp Leu His Ala Lys Met
65 70 75 80
Val Ala Ala Phe Val Glu Arg Arg Lys Gly Lys Ala Thr Pro Glu Gln
85 90 95
Ala Glu Lys Ile Val Gln Asp Ala Asn Tyr Phe Gly Thr Met Leu Val
100 105 110
Tyr Met Lys Glu Ala Asp Gly Met Val Ser Gly Ala Val His Ser Thr
115 120 125
Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Arg Pro Gly
130 135 140
Val His Leu Val Ser Gly Ala Phe Ile Met Gln Arg Gly Arg Asp Glu
145 150 155 160
Arg Tyr Leu Phe Ala Asp Asn Ala Ile Asn Ile Asp Pro Asp Ala Asp
165 170 175
Gln Leu Ala Glu Ile Ala Val Glu Ser Ala Lys Thr Ala Ala Leu Phe
180 185 190
Asp Ile Glu Pro Pro Arg Val Ala Leu Leu Ser Phe Ser Thr Lys Gly
195 200 205
Ser Ala Lys Gly Pro Gln Val Asp Lys Val Val Glu Ala Thr Lys Lys
210 215 220
Ala His Glu Leu Ala Pro Asp Leu Ala Leu Asp Gly Glu Leu Gln Phe
225 230 235 240
Asp Ala Ala Phe Val Pro Thr Val Ala Lys Gln Lys Ala Pro Asp Ser
245 250 255
Lys Val Ala Gly Glu Ala Asn Val Phe Ile Phe Pro Glu Leu Gln Ser
260 265 270
Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Phe Gly Gly Phe Glu Ala
275 280 285
Ile Gly Pro Ile Leu Gln Gly Leu Asn Gln Pro Val Ser Asp Leu Ser
290 295 300
Arg Gly Ala Asn Glu Glu Asp Val Tyr Lys Leu Ala Ile Ile Thr Ala
305 310 315 320
Ala Gln Thr Leu Leu
325
<210> 49
<211> 978
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(978)
<223> 植物乳杆菌 WCFS1; 基因 lp_0807 编码磷酸转乙酰酶
<400> 49
atg gat tta ttt gag tca tta gca caa aaa att act ggt aaa gat caa 48
Met Asp Leu Phe Glu Ser Leu Ala Gln Lys Ile Thr Gly Lys Asp Gln
1 5 10 15
aca att gtt ttc cct gaa gga act gaa ccc cga att gtc ggt gcg gca 96
Thr Ile Val Phe Pro Glu Gly Thr Glu Pro Arg Ile Val Gly Ala Ala
20 25 30
gcg cga tta gct gca gac ggc ttg gtt aag ccg att gtt tta ggt gca 144
Ala Arg Leu Ala Ala Asp Gly Leu Val Lys Pro Ile Val Leu Gly Ala
35 40 45
acg gac aaa gtt cag gct gtg gct aac gat ttg aat gcg gat tta aca 192
Thr Asp Lys Val Gln Ala Val Ala Asn Asp Leu Asn Ala Asp Leu Thr
50 55 60
ggc gtt caa gtc ctt gat cct gcg aca tac ccg gct gaa gat aag caa 240
Gly Val Gln Val Leu Asp Pro Ala Thr Tyr Pro Ala Glu Asp Lys Gln
65 70 75 80
gca atg ctt gat gcc ctc gtt gaa cgg cgg aaa ggt aag aat acg cca 288
Ala Met Leu Asp Ala Leu Val Glu Arg Arg Lys Gly Lys Asn Thr Pro
85 90 95
gaa caa gcg gct aaa atg ctg gaa gat gaa aac tac ttt ggc acg atg 336
Glu Gln Ala Ala Lys Met Leu Glu Asp Glu Asn Tyr Phe Gly Thr Met
100 105 110
ctc gtt tat atg ggc aaa gcg gat ggg atg gtt tca ggt gca atc cat 384
Leu Val Tyr Met Gly Lys Ala Asp Gly Met Val Ser Gly Ala Ile His
115 120 125
cca act ggt gat acg gta cgg cca gcg tta caa att att aag acc aag 432
Pro Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys
130 135 140
ccc ggt tca cac cga atc tcg ggt gca ttt atc atg caa aag ggt gag 480
Pro Gly Ser His Arg Ile Ser Gly Ala Phe Ile Met Gln Lys Gly Glu
145 150 155 160
gaa cgc tac gtc ttt gct gac tgt gcc atc aat att gat ccc gat gcc 528
Glu Arg Tyr Val Phe Ala Asp Cys Ala Ile Asn Ile Asp Pro Asp Ala
165 170 175
gat acg tta gcg gaa att gcc act cag agt gcg gct act gct aag gtc 576
Asp Thr Leu Ala Glu Ile Ala Thr Gln Ser Ala Ala Thr Ala Lys Val
180 185 190
ttc gat att gac ccg aaa gtt gcg atg ctc agc ttc tca act aag ggt 624
Phe Asp Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys Gly
195 200 205
tcg gct aag ggt gaa atg gtc act aaa gtg caa gaa gca acg gcc aag 672
Ser Ala Lys Gly Glu Met Val Thr Lys Val Gln Glu Ala Thr Ala Lys
210 215 220
gcg caa gct gct gaa ccg gaa ttg gct atc gat ggt gaa ctt caa ttt 720
Ala Gln Ala Ala Glu Pro Glu Leu Ala Ile Asp Gly Glu Leu Gln Phe
225 230 235 240
gac gcg gcc ttc gtt gaa aaa gtt ggt ttg caa aag gct cct ggt tcc 768
Asp Ala Ala Phe Val Glu Lys Val Gly Leu Gln Lys Ala Pro Gly Ser
245 250 255
aaa gta gct ggt cat gcc aat gtc ttt gta ttt cca gag ctt cag tct 816
Lys Val Ala Gly His Ala Asn Val Phe Val Phe Pro Glu Leu Gln Ser
260 265 270
ggt aat att ggc tat aag att gcg caa cga ttt ggt cat ttt gaa gcg 864
Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Phe Gly His Phe Glu Ala
275 280 285
gtg ggt cct gtc ttg caa ggc ctg aac aag ccg gtc tcc gac ttg tca 912
Val Gly Pro Val Leu Gln Gly Leu Asn Lys Pro Val Ser Asp Leu Ser
290 295 300
cgt gga tgc agt gaa gaa gac gtt tat aag gtt gcg att att aca gca 960
Arg Gly Cys Ser Glu Glu Asp Val Tyr Lys Val Ala Ile Ile Thr Ala
305 310 315 320
gcc caa gga tta gct taa 978
Ala Gln Gly Leu Ala
325
<210> 50
<211> 325
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 50
Met Asp Leu Phe Glu Ser Leu Ala Gln Lys Ile Thr Gly Lys Asp Gln
1 5 10 15
Thr Ile Val Phe Pro Glu Gly Thr Glu Pro Arg Ile Val Gly Ala Ala
20 25 30
Ala Arg Leu Ala Ala Asp Gly Leu Val Lys Pro Ile Val Leu Gly Ala
35 40 45
Thr Asp Lys Val Gln Ala Val Ala Asn Asp Leu Asn Ala Asp Leu Thr
50 55 60
Gly Val Gln Val Leu Asp Pro Ala Thr Tyr Pro Ala Glu Asp Lys Gln
65 70 75 80
Ala Met Leu Asp Ala Leu Val Glu Arg Arg Lys Gly Lys Asn Thr Pro
85 90 95
Glu Gln Ala Ala Lys Met Leu Glu Asp Glu Asn Tyr Phe Gly Thr Met
100 105 110
Leu Val Tyr Met Gly Lys Ala Asp Gly Met Val Ser Gly Ala Ile His
115 120 125
Pro Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys
130 135 140
Pro Gly Ser His Arg Ile Ser Gly Ala Phe Ile Met Gln Lys Gly Glu
145 150 155 160
Glu Arg Tyr Val Phe Ala Asp Cys Ala Ile Asn Ile Asp Pro Asp Ala
165 170 175
Asp Thr Leu Ala Glu Ile Ala Thr Gln Ser Ala Ala Thr Ala Lys Val
180 185 190
Phe Asp Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys Gly
195 200 205
Ser Ala Lys Gly Glu Met Val Thr Lys Val Gln Glu Ala Thr Ala Lys
210 215 220
Ala Gln Ala Ala Glu Pro Glu Leu Ala Ile Asp Gly Glu Leu Gln Phe
225 230 235 240
Asp Ala Ala Phe Val Glu Lys Val Gly Leu Gln Lys Ala Pro Gly Ser
245 250 255
Lys Val Ala Gly His Ala Asn Val Phe Val Phe Pro Glu Leu Gln Ser
260 265 270
Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Phe Gly His Phe Glu Ala
275 280 285
Val Gly Pro Val Leu Gln Gly Leu Asn Lys Pro Val Ser Asp Leu Ser
290 295 300
Arg Gly Cys Ser Glu Glu Asp Val Tyr Lys Val Ala Ile Ile Thr Ala
305 310 315 320
Ala Gln Gly Leu Ala
325
<210> 51
<211> 972
<212> DNA
<213> 戊糖片球菌（Pediococcus pentosaceus）
<220>
<221> CDS
<222> (1)..(972)
<223> 戊糖片球菌 ATCC 25745; 基因 PEPE_0337 编码磷酸转乙酰酶
<400> 51
atg gat tta ttt gaa ggt ttg aag tca aaa att aag ggc caa aat aga 48
Met Asp Leu Phe Glu Gly Leu Lys Ser Lys Ile Lys Gly Gln Asn Arg
1 5 10 15
aca gtt gta ttt ccc gaa ggt gaa gat gtt cga gtt tta gct gcc gct 96
Thr Val Val Phe Pro Glu Gly Glu Asp Val Arg Val Leu Ala Ala Ala
20 25 30
att cga cta gct gat gat ggc tta gtt caa tca atc tta ctc gga gtg 144
Ile Arg Leu Ala Asp Asp Gly Leu Val Gln Ser Ile Leu Leu Gly Val
35 40 45
gaa gct gat att aaa gct act gct gaa aag cat gga tta aac ctt gaa 192
Glu Ala Asp Ile Lys Ala Thr Ala Glu Lys His Gly Leu Asn Leu Glu
50 55 60
aat gtg caa att att gat ccg agc caa tat ccg gaa gcc gat caa aag 240
Asn Val Gln Ile Ile Asp Pro Ser Gln Tyr Pro Glu Ala Asp Gln Lys
65 70 75 80
gaa atg att gat aag tta gtt gaa cgt cgt aag ggt aaa aat acc gaa 288
Glu Met Ile Asp Lys Leu Val Glu Arg Arg Lys Gly Lys Asn Thr Glu
85 90 95
gaa cag gtt atc gaa atg ctc aaa gat gta aat tac ttc gga act atg 336
Glu Gln Val Ile Glu Met Leu Lys Asp Val Asn Tyr Phe Gly Thr Met
100 105 110
cta gtt tac tta ggc aag gcg gat gcc atg gta tct gga gca gtt cat 384
Leu Val Tyr Leu Gly Lys Ala Asp Ala Met Val Ser Gly Ala Val His
115 120 125
gct act ggt gat aca gtt cgc ccc gct ctt caa att atc aag act aaa 432
Ala Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys
130 135 140
cca gga atg cgt cga att agt gga gca ttt atc atg caa cgc gaa aat 480
Pro Gly Met Arg Arg Ile Ser Gly Ala Phe Ile Met Gln Arg Glu Asn
145 150 155 160
gaa cgc tat gtc ttt ggg gat gca gct att aat att gat ccc gat gca 528
Glu Arg Tyr Val Phe Gly Asp Ala Ala Ile Asn Ile Asp Pro Asp Ala
165 170 175
gaa aca ttg gct gaa att gcg att caa agt gca gaa act gct cgc cta 576
Glu Thr Leu Ala Glu Ile Ala Ile Gln Ser Ala Glu Thr Ala Arg Leu
180 185 190
ttt gac atc gac cct aag gtt gca atg tta agc ttt tca act aaa ggc 624
Phe Asp Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys Gly
195 200 205
tca gcg gct gga cca atg gta gat aaa gtt cgc gaa gct act aag gtt 672
Ser Ala Ala Gly Pro Met Val Asp Lys Val Arg Glu Ala Thr Lys Val
210 215 220
gcc caa gaa cgt gat cca aag acc cca tat gat ggt gaa ctt cag ttt 720
Ala Gln Glu Arg Asp Pro Lys Thr Pro Tyr Asp Gly Glu Leu Gln Phe
225 230 235 240
gat gca gcc ttt gtt cca gct gtt gga gca agt aag gct cca gat tca 768
Asp Ala Ala Phe Val Pro Ala Val Gly Ala Ser Lys Ala Pro Asp Ser
245 250 255
gaa att gca gga cat gcg aat gtc ttt atc ttc cct gaa ctt caa tct 816
Glu Ile Ala Gly His Ala Asn Val Phe Ile Phe Pro Glu Leu Gln Ser
260 265 270
gga aac att ggt tat aag att gct caa cgt cta ggc ggt ttt gaa gca 864
Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Gly Phe Glu Ala
275 280 285
att ggc cca atc ttg caa gga ctt aat gca ccg gtt tcc gat tta tct 912
Ile Gly Pro Ile Leu Gln Gly Leu Asn Ala Pro Val Ser Asp Leu Ser
290 295 300
cgt gga gct agt gaa gaa gat gtc tat aaa gta gca att att acc gca 960
Arg Gly Ala Ser Glu Glu Asp Val Tyr Lys Val Ala Ile Ile Thr Ala
305 310 315 320
gct caa gtt tag 972
Ala Gln Val
<210> 52
<211> 323
<212> PRT
<213> 戊糖片球菌（Pediococcus pentosaceus）
<400> 52
Met Asp Leu Phe Glu Gly Leu Lys Ser Lys Ile Lys Gly Gln Asn Arg
1 5 10 15
Thr Val Val Phe Pro Glu Gly Glu Asp Val Arg Val Leu Ala Ala Ala
20 25 30
Ile Arg Leu Ala Asp Asp Gly Leu Val Gln Ser Ile Leu Leu Gly Val
35 40 45
Glu Ala Asp Ile Lys Ala Thr Ala Glu Lys His Gly Leu Asn Leu Glu
50 55 60
Asn Val Gln Ile Ile Asp Pro Ser Gln Tyr Pro Glu Ala Asp Gln Lys
65 70 75 80
Glu Met Ile Asp Lys Leu Val Glu Arg Arg Lys Gly Lys Asn Thr Glu
85 90 95
Glu Gln Val Ile Glu Met Leu Lys Asp Val Asn Tyr Phe Gly Thr Met
100 105 110
Leu Val Tyr Leu Gly Lys Ala Asp Ala Met Val Ser Gly Ala Val His
115 120 125
Ala Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys
130 135 140
Pro Gly Met Arg Arg Ile Ser Gly Ala Phe Ile Met Gln Arg Glu Asn
145 150 155 160
Glu Arg Tyr Val Phe Gly Asp Ala Ala Ile Asn Ile Asp Pro Asp Ala
165 170 175
Glu Thr Leu Ala Glu Ile Ala Ile Gln Ser Ala Glu Thr Ala Arg Leu
180 185 190
Phe Asp Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys Gly
195 200 205
Ser Ala Ala Gly Pro Met Val Asp Lys Val Arg Glu Ala Thr Lys Val
210 215 220
Ala Gln Glu Arg Asp Pro Lys Thr Pro Tyr Asp Gly Glu Leu Gln Phe
225 230 235 240
Asp Ala Ala Phe Val Pro Ala Val Gly Ala Ser Lys Ala Pro Asp Ser
245 250 255
Glu Ile Ala Gly His Ala Asn Val Phe Ile Phe Pro Glu Leu Gln Ser
260 265 270
Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Gly Phe Glu Ala
275 280 285
Ile Gly Pro Ile Leu Gln Gly Leu Asn Ala Pro Val Ser Asp Leu Ser
290 295 300
Arg Gly Ala Ser Glu Glu Asp Val Tyr Lys Val Ala Ile Ile Thr Ala
305 310 315 320
Ala Gln Val
<210> 53
<211> 981
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(981)
<223> 肠膜明串珠菌肠膜亚种（Leuconostoc mesenteroides subsp.mesenteroides） ATCC 8293; 基因
LEUM_1466 编码磷酸转乙酰酶
<400> 53
atg gaa ctt ttt gag caa tta aaa aat aaa att acc ggt caa aat aag 48
Met Glu Leu Phe Glu Gln Leu Lys Asn Lys Ile Thr Gly Gln Asn Lys
1 5 10 15
aca att gta ttt ccg gaa ggt gaa gat ccc cgt atc cag ggc gca gct 96
Thr Ile Val Phe Pro Glu Gly Glu Asp Pro Arg Ile Gln Gly Ala Ala
20 25 30
atc aga tta gca gct gat aat ttg atc gag cca ata ttg ttg ggg gat 144
Ile Arg Leu Ala Ala Asp Asn Leu Ile Glu Pro Ile Leu Leu Gly Asp
35 40 45
gca cag gaa att tca aaa act gcc caa gcc cat aac ttt gat tta tcg 192
Ala Gln Glu Ile Ser Lys Thr Ala Gln Ala His Asn Phe Asp Leu Ser
50 55 60
aac ata gag acg att gat cct gct tca tat gat gag aat gaa ttg gca 240
Asn Ile Glu Thr Ile Asp Pro Ala Ser Tyr Asp Glu Asn Glu Leu Ala
65 70 75 80
aaa tta aat gcg aca ctt gtc gaa agg cgc aaa ggg aaa aca gac gct 288
Lys Leu Asn Ala Thr Leu Val Glu Arg Arg Lys Gly Lys Thr Asp Ala
85 90 95
gag aca gct gcc aaa tgg cta caa aat gtt aac tat ttt ggg aca atg 336
Glu Thr Ala Ala Lys Trp Leu Gln Asn Val Asn Tyr Phe Gly Thr Met
100 105 110
ctg gtt tat act ggc aaa gct gac ggt atg gtg tca ggc gca gta cat 384
Leu Val Tyr Thr Gly Lys Ala Asp Gly Met Val Ser Gly Ala Val His
115 120 125
cca act ggt gat aca gtt cgt cct gcg ttg cag atc att aaa acg gcg 432
Pro Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Ala
130 135 140
cct ggc tca tca cgt att tct ggt gca ttc att atg caa aaa ggc gaa 480
Pro Gly Ser Ser Arg Ile Ser Gly Ala Phe Ile Met Gln Lys Gly Glu
145 150 155 160
gag cgt tat gta ttt gcg gac gct gcc att aac att gac att gat tcg 528
Glu Arg Tyr Val Phe Ala Asp Ala Ala Ile Asn Ile Asp Ile Asp Ser
165 170 175
gac acg atg gct gaa att gca ata caa tca gcc cac aca gct caa gtg 576
Asp Thr Met Ala Glu Ile Ala Ile Gln Ser Ala His Thr Ala Gln Val
180 185 190
ttt gat att gaa cca aaa gtt gcg atg ctt tca ttt tca act aag ggc 624
Phe Asp Ile Glu Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys Gly
195 200 205
tcc gcg aaa tca ccc ttg gtc gat aag gtg gca act gcc act gca ttg 672
Ser Ala Lys Ser Pro Leu Val Asp Lys Val Ala Thr Ala Thr Ala Leu
210 215 220
gct aag aag tta gca cct gaa ctg gcg gac tca att gat gga gag tta 720
Ala Lys Lys Leu Ala Pro Glu Leu Ala Asp Ser Ile Asp Gly Glu Leu
225 230 235 240
cag ttt gat gct gcg ttt gtt gaa tct gtt gct gcc gca aag gcg cct 768
Gln Phe Asp Ala Ala Phe Val Glu Ser Val Ala Ala Ala Lys Ala Pro
245 250 255
gat tca aaa gtc gca ggt aaa gcg aac aca ttt att ttc cca agt cta 816
Asp Ser Lys Val Ala Gly Lys Ala Asn Thr Phe Ile Phe Pro Ser Leu
260 265 270
gaa gca ggt aat att ggc tat aaa att gca caa cgc ttg ggt ggg ttt 864
Glu Ala Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Gly Phe
275 280 285
gaa gcg att gga cct att tta caa ggc tta gca aaa cct gtt tct gat 912
Glu Ala Ile Gly Pro Ile Leu Gln Gly Leu Ala Lys Pro Val Ser Asp
290 295 300
tta tcg cga gga gcc aat gaa gag gat gtt tat aaa gta gct att att 960
Leu Ser Arg Gly Ala Asn Glu Glu Asp Val Tyr Lys Val Ala Ile Ile
305 310 315 320
act gct gcg caa gca tta taa 981
Thr Ala Ala Gln Ala Leu
325
<210> 54
<211> 326
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 54
Met Glu Leu Phe Glu Gln Leu Lys Asn Lys Ile Thr Gly Gln Asn Lys
1 5 10 15
Thr Ile Val Phe Pro Glu Gly Glu Asp Pro Arg Ile Gln Gly Ala Ala
20 25 30
Ile Arg Leu Ala Ala Asp Asn Leu Ile Glu Pro Ile Leu Leu Gly Asp
35 40 45
Ala Gln Glu Ile Ser Lys Thr Ala Gln Ala His Asn Phe Asp Leu Ser
50 55 60
Asn Ile Glu Thr Ile Asp Pro Ala Ser Tyr Asp Glu Asn Glu Leu Ala
65 70 75 80
Lys Leu Asn Ala Thr Leu Val Glu Arg Arg Lys Gly Lys Thr Asp Ala
85 90 95
Glu Thr Ala Ala Lys Trp Leu Gln Asn Val Asn Tyr Phe Gly Thr Met
100 105 110
Leu Val Tyr Thr Gly Lys Ala Asp Gly Met Val Ser Gly Ala Val His
115 120 125
Pro Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Ala
130 135 140
Pro Gly Ser Ser Arg Ile Ser Gly Ala Phe Ile Met Gln Lys Gly Glu
145 150 155 160
Glu Arg Tyr Val Phe Ala Asp Ala Ala Ile Asn Ile Asp Ile Asp Ser
165 170 175
Asp Thr Met Ala Glu Ile Ala Ile Gln Ser Ala His Thr Ala Gln Val
180 185 190
Phe Asp Ile Glu Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys Gly
195 200 205
Ser Ala Lys Ser Pro Leu Val Asp Lys Val Ala Thr Ala Thr Ala Leu
210 215 220
Ala Lys Lys Leu Ala Pro Glu Leu Ala Asp Ser Ile Asp Gly Glu Leu
225 230 235 240
Gln Phe Asp Ala Ala Phe Val Glu Ser Val Ala Ala Ala Lys Ala Pro
245 250 255
Asp Ser Lys Val Ala Gly Lys Ala Asn Thr Phe Ile Phe Pro Ser Leu
260 265 270
Glu Ala Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Gly Phe
275 280 285
Glu Ala Ile Gly Pro Ile Leu Gln Gly Leu Ala Lys Pro Val Ser Asp
290 295 300
Leu Ser Arg Gly Ala Asn Glu Glu Asp Val Tyr Lys Val Ala Ile Ile
305 310 315 320
Thr Ala Ala Gln Ala Leu
325
<210> 55
<211> 984
<212> DNA
<213> 嗜热链球菌（Streptococcus thermophilus）
<220>
<221> CDS
<222> (1)..(984)
<223> 嗜热链球菌 CNRZ1066; 基因 str1455 编码磷酸转乙酰酶
<400> 55
atg cgt agt cta ttt ggt gaa ctt cgt gaa cat att tca ggg aaa gga 48
Met Arg Ser Leu Phe Gly Glu Leu Arg Glu His Ile Ser Gly Lys Gly
1 5 10 15
gtt agg att gtt ttc cct gag ggt aat gat gac cgt gtg gtt cgt gca 96
Val Arg Ile Val Phe Pro Glu Gly Asn Asp Asp Arg Val Val Arg Ala
20 25 30
gct gcc cgt ttg aaa ttt gaa gaa tta gtt gat cct att att ttg gga 144
Ala Ala Arg Leu Lys Phe Glu Glu Leu Val Asp Pro Ile Ile Leu Gly
35 40 45
gac cct aca gag gtt cac aaa atc ttg aac gat ctt ggt ttt gta gac 192
Asp Pro Thr Glu Val His Lys Ile Leu Asn Asp Leu Gly Phe Val Asp
50 55 60
ttt aac tgt act att att aat cct gaa acc tat gaa ggc ttc gag gaa 240
Phe Asn Cys Thr Ile Ile Asn Pro Glu Thr Tyr Glu Gly Phe Glu Glu
65 70 75 80
atg aag gaa aaa ttt gtt gaa gtc cgt aaa ggg aaa gcg aca att gaa 288
Met Lys Glu Lys Phe Val Glu Val Arg Lys Gly Lys Ala Thr Ile Glu
85 90 95
gat gca gac aag ttg ctt cgt gat gtc aac tat ttt ggt gtt atg cta 336
Asp Ala Asp Lys Leu Leu Arg Asp Val Asn Tyr Phe Gly Val Met Leu
100 105 110
gtt aaa ctt ggt tta gca gat gga atg gtt tca ggc gcg att cac tca 384
Val Lys Leu Gly Leu Ala Asp Gly Met Val Ser Gly Ala Ile His Ser
115 120 125
aca gcg gat acc gtt cgt cct gct ctt caa att atc aaa acg aaa cct 432
Thr Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Pro
130 135 140
ggt att tct tgt aca tca ggt gtc ttc ctt atg aac cgt gaa gat acc 480
Gly Ile Ser Cys Thr Ser Gly Val Phe Leu Met Asn Arg Glu Asp Thr
145 150 155 160
aat cat cgc ttg atg ttt gcc gac tgt gct atc aat att gaa cca aat 528
Asn His Arg Leu Met Phe Ala Asp Cys Ala Ile Asn Ile Glu Pro Asn
165 170 175
tct caa gaa tta gca gaa att gca att aat act gca gaa aca gct aaa 576
Ser Gln Glu Leu Ala Glu Ile Ala Ile Asn Thr Ala Glu Thr Ala Lys
180 185 190
gtt ttt ggt att gat cct aaa gtg gca atg ctt agc ttc tca aca aaa 624
Val Phe Gly Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys
195 200 205
ggt tcc gct aaa tca cca aaa gtt gac agg gtt gtt caa gca aca aat 672
Gly Ser Ala Lys Ser Pro Lys Val Asp Arg Val Val Gln Ala Thr Asn
210 215 220
att gct aaa gaa atg cgt cct gac ttg gct att gat ggt gag ttg cag 720
Ile Ala Lys Glu Met Arg Pro Asp Leu Ala Ile Asp Gly Glu Leu Gln
225 230 235 240
ttt gat gca gcc ttt gtt cca gca aca gca gct att aag gca cca gac 768
Phe Asp Ala Ala Phe Val Pro Ala Thr Ala Ala Ile Lys Ala Pro Asp
245 250 255
agt gac gta gca gga caa gct aac gtc ttt gtc ttc cca tgt tta cag 816
Ser Asp Val Ala Gly Gln Ala Asn Val Phe Val Phe Pro Cys Leu Gln
260 265 270
gct ggt aat att ggt tat aag att gcc caa cgt ttg gga atg ttt gag 864
Ala Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Met Phe Glu
275 280 285
gct atc gga cca atc tta caa ggt ctt aat aaa cca gta aac gat ctt 912
Ala Ile Gly Pro Ile Leu Gln Gly Leu Asn Lys Pro Val Asn Asp Leu
290 295 300
tct cgt ggt gct agt gct gaa gat att tac aac tta gct att atc aca 960
Ser Arg Gly Ala Ser Ala Glu Asp Ile Tyr Asn Leu Ala Ile Ile Thr
305 310 315 320
gct gct caa gct ctt aat aat taa 984
Ala Ala Gln Ala Leu Asn Asn
325
<210> 56
<211> 327
<212> PRT
<213> 嗜热链球菌（Streptococcus thermophilus）
<400> 56
Met Arg Ser Leu Phe Gly Glu Leu Arg Glu His Ile Ser Gly Lys Gly
1 5 10 15
Val Arg Ile Val Phe Pro Glu Gly Asn Asp Asp Arg Val Val Arg Ala
20 25 30
Ala Ala Arg Leu Lys Phe Glu Glu Leu Val Asp Pro Ile Ile Leu Gly
35 40 45
Asp Pro Thr Glu Val His Lys Ile Leu Asn Asp Leu Gly Phe Val Asp
50 55 60
Phe Asn Cys Thr Ile Ile Asn Pro Glu Thr Tyr Glu Gly Phe Glu Glu
65 70 75 80
Met Lys Glu Lys Phe Val Glu Val Arg Lys Gly Lys Ala Thr Ile Glu
85 90 95
Asp Ala Asp Lys Leu Leu Arg Asp Val Asn Tyr Phe Gly Val Met Leu
100 105 110
Val Lys Leu Gly Leu Ala Asp Gly Met Val Ser Gly Ala Ile His Ser
115 120 125
Thr Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Pro
130 135 140
Gly Ile Ser Cys Thr Ser Gly Val Phe Leu Met Asn Arg Glu Asp Thr
145 150 155 160
Asn His Arg Leu Met Phe Ala Asp Cys Ala Ile Asn Ile Glu Pro Asn
165 170 175
Ser Gln Glu Leu Ala Glu Ile Ala Ile Asn Thr Ala Glu Thr Ala Lys
180 185 190
Val Phe Gly Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys
195 200 205
Gly Ser Ala Lys Ser Pro Lys Val Asp Arg Val Val Gln Ala Thr Asn
210 215 220
Ile Ala Lys Glu Met Arg Pro Asp Leu Ala Ile Asp Gly Glu Leu Gln
225 230 235 240
Phe Asp Ala Ala Phe Val Pro Ala Thr Ala Ala Ile Lys Ala Pro Asp
245 250 255
Ser Asp Val Ala Gly Gln Ala Asn Val Phe Val Phe Pro Cys Leu Gln
260 265 270
Ala Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Met Phe Glu
275 280 285
Ala Ile Gly Pro Ile Leu Gln Gly Leu Asn Lys Pro Val Asn Asp Leu
290 295 300
Ser Arg Gly Ala Ser Ala Glu Asp Ile Tyr Asn Leu Ala Ile Ile Thr
305 310 315 320
Ala Ala Gln Ala Leu Asn Asn
325
<210> 57
<211> 990
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(990)
<223> 酒类酒球菌 PSU-1; 基因 OEOE_1435 编码磷酸转乙酰酶
<400> 57
atg aca aat tta ttt act gag ttg gct gat aaa att aag gaa aaa aaa 48
Met Thr Asn Leu Phe Thr Glu Leu Ala Asp Lys Ile Lys Glu Lys Lys
1 5 10 15
ctg aga ctc gtt ttt ccg gaa ggc gat gat tca aga gtc cag ggg gcg 96
Leu Arg Leu Val Phe Pro Glu Gly Asp Asp Ser Arg Val Gln Gly Ala
20 25 30
gcc atc cgt tta aaa aaa gat ggt tta cta acg ccg ata tta ttg gga 144
Ala Ile Arg Leu Lys Lys Asp Gly Leu Leu Thr Pro Ile Leu Leu Gly
35 40 45
gat cag gca gcg gtc gaa aag aca gct gca gat aat cat ttc gac ctg 192
Asp Gln Ala Ala Val Glu Lys Thr Ala Ala Asp Asn His Phe Asp Leu
50 55 60
agt ggc atc aag ctt atc gat ccg ctt aaa ttc gat gag aag cag ttc 240
Ser Gly Ile Lys Leu Ile Asp Pro Leu Lys Phe Asp Glu Lys Gln Phe
65 70 75 80
aat caa ttg atc gat aaa ctg gtt acc cga cga aaa ggg aaa act gac 288
Asn Gln Leu Ile Asp Lys Leu Val Thr Arg Arg Lys Gly Lys Thr Asp
85 90 95
ccg caa acg gta gct atg tgg ctg cag gaa gta aat tat ttc ggt acc 336
Pro Gln Thr Val Ala Met Trp Leu Gln Glu Val Asn Tyr Phe Gly Thr
100 105 110
atg ctt gtt tat acc ggc aag gcc gac gca atg gtt tcc ggt gct gtt 384
Met Leu Val Tyr Thr Gly Lys Ala Asp Ala Met Val Ser Gly Ala Val
115 120 125
cat cca acc ggc gat acg gtt cgt ccc gct ttg cag att atc aag aca 432
His Pro Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr
130 135 140
gct cct ggt gtc agt cgt att tcc ggt gct ttt att atg caa aaa aat 480
Ala Pro Gly Val Ser Arg Ile Ser Gly Ala Phe Ile Met Gln Lys Asn
145 150 155 160
gat gaa cgc tat ctg ttt gcc gat tcg gca att aat atc gaa ctt gat 528
Asp Glu Arg Tyr Leu Phe Ala Asp Ser Ala Ile Asn Ile Glu Leu Asp
165 170 175
gca caa acc atg gct gag att gcc gtt caa tca gcc cat acg gct aaa 576
Ala Gln Thr Met Ala Glu Ile Ala Val Gln Ser Ala His Thr Ala Lys
180 185 190
ata ttt gga atc gat cca aaa gtt gcc atg ctg agt ttt tca acc aaa 624
Ile Phe Gly Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys
195 200 205
gga tcg gca aaa cat gca ttg gtc gat aaa gtt gct cag gca act aag 672
Gly Ser Ala Lys His Ala Leu Val Asp Lys Val Ala Gln Ala Thr Lys
210 215 220
ctg gcg cag gag atg gca ccg gat tta gct gat aat att gat ggt gaa 720
Leu Ala Gln Glu Met Ala Pro Asp Leu Ala Asp Asn Ile Asp Gly Glu
225 230 235 240
tta caa ttc gat gca gct ttt gtc gaa tcg gtt gcc aaa tta aaa gct 768
Leu Gln Phe Asp Ala Ala Phe Val Glu Ser Val Ala Lys Leu Lys Ala
245 250 255
ccg gga tca aaa gtg gcc ggt cat gcg aac gtt ttc att ttt cca agt 816
Pro Gly Ser Lys Val Ala Gly His Ala Asn Val Phe Ile Phe Pro Ser
260 265 270
ttg gaa gcc gga aat att ggt tat aaa att gcc caa cgc ttg ggt gga 864
Leu Glu Ala Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Gly
275 280 285
tac gaa gca att ggt cct att ttg caa gga ttg gca gca cct gtt tcc 912
Tyr Glu Ala Ile Gly Pro Ile Leu Gln Gly Leu Ala Ala Pro Val Ser
290 295 300
gat ctg tcg cgt gga gca agt caa gaa gac gta tat aaa gtt gca att 960
Asp Leu Ser Arg Gly Ala Ser Gln Glu Asp Val Tyr Lys Val Ala Ile
305 310 315 320
att act gct gct cag gct tta aaa aat tag 990
Ile Thr Ala Ala Gln Ala Leu Lys Asn
325
<210> 58
<211> 329
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 58
Met Thr Asn Leu Phe Thr Glu Leu Ala Asp Lys Ile Lys Glu Lys Lys
1 5 10 15
Leu Arg Leu Val Phe Pro Glu Gly Asp Asp Ser Arg Val Gln Gly Ala
20 25 30
Ala Ile Arg Leu Lys Lys Asp Gly Leu Leu Thr Pro Ile Leu Leu Gly
35 40 45
Asp Gln Ala Ala Val Glu Lys Thr Ala Ala Asp Asn His Phe Asp Leu
50 55 60
Ser Gly Ile Lys Leu Ile Asp Pro Leu Lys Phe Asp Glu Lys Gln Phe
65 70 75 80
Asn Gln Leu Ile Asp Lys Leu Val Thr Arg Arg Lys Gly Lys Thr Asp
85 90 95
Pro Gln Thr Val Ala Met Trp Leu Gln Glu Val Asn Tyr Phe Gly Thr
100 105 110
Met Leu Val Tyr Thr Gly Lys Ala Asp Ala Met Val Ser Gly Ala Val
115 120 125
His Pro Thr Gly Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr
130 135 140
Ala Pro Gly Val Ser Arg Ile Ser Gly Ala Phe Ile Met Gln Lys Asn
145 150 155 160
Asp Glu Arg Tyr Leu Phe Ala Asp Ser Ala Ile Asn Ile Glu Leu Asp
165 170 175
Ala Gln Thr Met Ala Glu Ile Ala Val Gln Ser Ala His Thr Ala Lys
180 185 190
Ile Phe Gly Ile Asp Pro Lys Val Ala Met Leu Ser Phe Ser Thr Lys
195 200 205
Gly Ser Ala Lys His Ala Leu Val Asp Lys Val Ala Gln Ala Thr Lys
210 215 220
Leu Ala Gln Glu Met Ala Pro Asp Leu Ala Asp Asn Ile Asp Gly Glu
225 230 235 240
Leu Gln Phe Asp Ala Ala Phe Val Glu Ser Val Ala Lys Leu Lys Ala
245 250 255
Pro Gly Ser Lys Val Ala Gly His Ala Asn Val Phe Ile Phe Pro Ser
260 265 270
Leu Glu Ala Gly Asn Ile Gly Tyr Lys Ile Ala Gln Arg Leu Gly Gly
275 280 285
Tyr Glu Ala Ile Gly Pro Ile Leu Gln Gly Leu Ala Ala Pro Val Ser
290 295 300
Asp Leu Ser Arg Gly Ala Ser Gln Glu Asp Val Tyr Lys Val Ala Ile
305 310 315 320
Ile Thr Ala Ala Gln Ala Leu Lys Asn
325
<210> 59
<211> 972
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（GTG）处的tRNA插入Met而不是val
<220>
<221> CDS
<222> (1)..(972)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_1406 编码磷酸转乙酰酶
<400> 59
gtg agt aca ttt ttt gac ggg ata aaa gaa aaa gtg cgg gga cat aac 48
Val Ser Thr Phe Phe Asp Gly Ile Lys Glu Lys Val Arg Gly His Asn
1 5 10 15
aaa acg att gtt ttt ccg gaa agt tcg gat gaa agg gtt tta aca gcc 96
Lys Thr Ile Val Phe Pro Glu Ser Ser Asp Glu Arg Val Leu Thr Ala
20 25 30
gtc tcc cgg ttt gca gcc gat gaa gta ctc cgg ccg gtt tta atc ggg 144
Val Ser Arg Phe Ala Ala Asp Glu Val Leu Arg Pro Val Leu Ile Gly
35 40 45
aac aag gat aaa att cag gca aaa gcc cag gcc atc ggt gtt tct gtc 192
Asn Lys Asp Lys Ile Gln Ala Lys Ala Gln Ala Ile Gly Val Ser Val
50 55 60
aag ggc gtt aca gtg tat gac ccg gaa gac tat gcc ggt ttt gaa gaa 240
Lys Gly Val Thr Val Tyr Asp Pro Glu Asp Tyr Ala Gly Phe Glu Glu
65 70 75 80
atg gtg cag gct ttt gtg gaa cga aga aaa ggc aaa gcg acg gag gaa 288
Met Val Gln Ala Phe Val Glu Arg Arg Lys Gly Lys Ala Thr Glu Glu
85 90 95
gat gcg cgc caa att tta aaa gac gtc aat tat ttc ggc acc atg ctc 336
Asp Ala Arg Gln Ile Leu Lys Asp Val Asn Tyr Phe Gly Thr Met Leu
100 105 110
gtc tat ttg aaa aaa gcg gac ggt ctg gta agc ggt gcc atc cat tca 384
Val Tyr Leu Lys Lys Ala Asp Gly Leu Val Ser Gly Ala Ile His Ser
115 120 125
acg gca gat aca gtc cgg ccg gca ttg cag atc att aaa aca aaa ccg 432
Thr Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Pro
130 135 140
ggc gtg aaa aaa gtt tct ggc gtt ttc att atg gtg agg ggc gaa gag 480
Gly Val Lys Lys Val Ser Gly Val Phe Ile Met Val Arg Gly Glu Glu
145 150 155 160
aaa ttt gtt ttc gcc gat tgt gca atc aat att tca ccg gac agc caa 528
Lys Phe Val Phe Ala Asp Cys Ala Ile Asn Ile Ser Pro Asp Ser Gln
165 170 175
gac ttg gcg gaa acg gcc gtg gag agc gcg aaa act gcc cgg aca ttt 576
Asp Leu Ala Glu Thr Ala Val Glu Ser Ala Lys Thr Ala Arg Thr Phe
180 185 190
gat att gaa ccg agg att gcg atg ttg agc ttt tca aca aga ggt tcc 624
Asp Ile Glu Pro Arg Ile Ala Met Leu Ser Phe Ser Thr Arg Gly Ser
195 200 205
gcc cat tcc gat gag acg gag aaa gtg gcg cag gca gtc cga ttg gct 672
Ala His Ser Asp Glu Thr Glu Lys Val Ala Gln Ala Val Arg Leu Ala
210 215 220
aaa gag cgc gat ccg gag ctt gtc atg gac ggc gag atg cag ttt gat 720
Lys Glu Arg Asp Pro Glu Leu Val Met Asp Gly Glu Met Gln Phe Asp
225 230 235 240
gcc gcg tat gtg ccg gct gtg gcg caa aag aaa gcg ccg gat tcc gtg 768
Ala Ala Tyr Val Pro Ala Val Ala Gln Lys Lys Ala Pro Asp Ser Val
245 250 255
att cag ggg aat gca act gtg ttt att ttt cca aat ctg gat gcg ggg 816
Ile Gln Gly Asn Ala Thr Val Phe Ile Phe Pro Asn Leu Asp Ala Gly
260 265 270
aat atc ggt tat aaa atg gcc cag cgt ctt ggc ggt ttt gaa gca gtc 864
Asn Ile Gly Tyr Lys Met Ala Gln Arg Leu Gly Gly Phe Glu Ala Val
275 280 285
ggt ccg att ttg caa ggg ctg aat gcg cct gtc aac gat ttg tcg cgc 912
Gly Pro Ile Leu Gln Gly Leu Asn Ala Pro Val Asn Asp Leu Ser Arg
290 295 300
ggc tgc aat gcg gaa gat gtt tat cat ttg gct ttg att tcc gca gca 960
Gly Cys Asn Ala Glu Asp Val Tyr His Leu Ala Leu Ile Ser Ala Ala
305 310 315 320
caa agt ttg taa 972
Gln Ser Leu
<210> 60
<211> 323
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 60
Val Ser Thr Phe Phe Asp Gly Ile Lys Glu Lys Val Arg Gly His Asn
1 5 10 15
Lys Thr Ile Val Phe Pro Glu Ser Ser Asp Glu Arg Val Leu Thr Ala
20 25 30
Val Ser Arg Phe Ala Ala Asp Glu Val Leu Arg Pro Val Leu Ile Gly
35 40 45
Asn Lys Asp Lys Ile Gln Ala Lys Ala Gln Ala Ile Gly Val Ser Val
50 55 60
Lys Gly Val Thr Val Tyr Asp Pro Glu Asp Tyr Ala Gly Phe Glu Glu
65 70 75 80
Met Val Gln Ala Phe Val Glu Arg Arg Lys Gly Lys Ala Thr Glu Glu
85 90 95
Asp Ala Arg Gln Ile Leu Lys Asp Val Asn Tyr Phe Gly Thr Met Leu
100 105 110
Val Tyr Leu Lys Lys Ala Asp Gly Leu Val Ser Gly Ala Ile His Ser
115 120 125
Thr Ala Asp Thr Val Arg Pro Ala Leu Gln Ile Ile Lys Thr Lys Pro
130 135 140
Gly Val Lys Lys Val Ser Gly Val Phe Ile Met Val Arg Gly Glu Glu
145 150 155 160
Lys Phe Val Phe Ala Asp Cys Ala Ile Asn Ile Ser Pro Asp Ser Gln
165 170 175
Asp Leu Ala Glu Thr Ala Val Glu Ser Ala Lys Thr Ala Arg Thr Phe
180 185 190
Asp Ile Glu Pro Arg Ile Ala Met Leu Ser Phe Ser Thr Arg Gly Ser
195 200 205
Ala His Ser Asp Glu Thr Glu Lys Val Ala Gln Ala Val Arg Leu Ala
210 215 220
Lys Glu Arg Asp Pro Glu Leu Val Met Asp Gly Glu Met Gln Phe Asp
225 230 235 240
Ala Ala Tyr Val Pro Ala Val Ala Gln Lys Lys Ala Pro Asp Ser Val
245 250 255
Ile Gln Gly Asn Ala Thr Val Phe Ile Phe Pro Asn Leu Asp Ala Gly
260 265 270
Asn Ile Gly Tyr Lys Met Ala Gln Arg Leu Gly Gly Phe Glu Ala Val
275 280 285
Gly Pro Ile Leu Gln Gly Leu Asn Ala Pro Val Asn Asp Leu Ser Arg
290 295 300
Gly Cys Asn Ala Glu Asp Val Tyr His Leu Ala Leu Ile Ser Ala Ala
305 310 315 320
Gln Ser Leu
<210> 61
<211> 2712
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(2712)
<223> 乳酸乳球菌乳脂亚种（Lactococcus lactis subsp. cremoris） MG1363; 基因llmg_2432
编码乙醇脱氢酶
<400> 61
atg gca act aaa aaa gcc gct cca gct gca aag aaa gtt tta agc gct 48
Met Ala Thr Lys Lys Ala Ala Pro Ala Ala Lys Lys Val Leu Ser Ala
1 5 10 15
gaa gaa aaa gcc gca aaa ttc caa gaa gct gtc gct tat act gat caa 96
Glu Glu Lys Ala Ala Lys Phe Gln Glu Ala Val Ala Tyr Thr Asp Gln
20 25 30
tta gtc aaa aaa gct caa gct gca gtt ctt aaa ttt gaa gga tac aca 144
Leu Val Lys Lys Ala Gln Ala Ala Val Leu Lys Phe Glu Gly Tyr Thr
35 40 45
caa act caa gtt gat act att gtt gct gca atg gct ctt gca gca agc 192
Gln Thr Gln Val Asp Thr Ile Val Ala Ala Met Ala Leu Ala Ala Ser
50 55 60
aaa cat tct ctg gaa ctc gct cac gaa gcc gtt aat gaa act ggc cgt 240
Lys His Ser Leu Glu Leu Ala His Glu Ala Val Asn Glu Thr Gly Arg
65 70 75 80
gga gtt gtt gag gac aaa gat aca aaa aac cat ttt gct tct gaa tct 288
Gly Val Val Glu Asp Lys Asp Thr Lys Asn His Phe Ala Ser Glu Ser
85 90 95
gtt tat aat gca atc aaa aat gat aaa aca gtt ggc gtt atc gct gaa 336
Val Tyr Asn Ala Ile Lys Asn Asp Lys Thr Val Gly Val Ile Ala Glu
100 105 110
aac aaa gtt gct ggt tct gtt gaa atc gca agc ccc ctt gga gta ctt 384
Asn Lys Val Ala Gly Ser Val Glu Ile Ala Ser Pro Leu Gly Val Leu
115 120 125
gct ggt att gtc cca aca act aat cca aca tca aca gcc atc ttt aaa 432
Ala Gly Ile Val Pro Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys
130 135 140
tca tta tta act gca aag aca cgt aat gct att gtc ttt gcc ttt cac 480
Ser Leu Leu Thr Ala Lys Thr Arg Asn Ala Ile Val Phe Ala Phe His
145 150 155 160
cca caa gca caa aaa tgc tca agc cat gcg gca aaa att gtt tat gat 528
Pro Gln Ala Gln Lys Cys Ser Ser His Ala Ala Lys Ile Val Tyr Asp
165 170 175
gct gcg att gaa gct ggt gca cct gaa gac ttt att caa tgg att gaa 576
Ala Ala Ile Glu Ala Gly Ala Pro Glu Asp Phe Ile Gln Trp Ile Glu
180 185 190
gta ccc agt ctt gat atg acg act gct ttg att caa aat aga gga att 624
Val Pro Ser Leu Asp Met Thr Thr Ala Leu Ile Gln Asn Arg Gly Ile
195 200 205
gct aca att ctt gca act ggt ggt cca ggt atg gtc aat gcc gcg ctt 672
Ala Thr Ile Leu Ala Thr Gly Gly Pro Gly Met Val Asn Ala Ala Leu
210 215 220
aag tct ggt aat cct tca ctt ggt gta ggt gct ggt aat ggt gca gtt 720
Lys Ser Gly Asn Pro Ser Leu Gly Val Gly Ala Gly Asn Gly Ala Val
225 230 235 240
tat gtt gat gca act gca aat atc gat cgt gct gtt gaa gat ctt ttg 768
Tyr Val Asp Ala Thr Ala Asn Ile Asp Arg Ala Val Glu Asp Leu Leu
245 250 255
ctt tca aaa cgt ttt gat aac gga atg att tgt gcg act gaa aac tct 816
Leu Ser Lys Arg Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser
260 265 270
gca gtt att gat gca tca atc tat gat gaa ttt gtc gct aaa atg caa 864
Ala Val Ile Asp Ala Ser Ile Tyr Asp Glu Phe Val Ala Lys Met Gln
275 280 285
gcg caa ggc gct tat atg gtt cct aaa aaa gat tac aag gca att gaa 912
Ala Gln Gly Ala Tyr Met Val Pro Lys Lys Asp Tyr Lys Ala Ile Glu
290 295 300
agt ttt gtt ttc gtt gaa cgt gct ggt gaa ggt ttt ggt gta act ggt 960
Ser Phe Val Phe Val Glu Arg Ala Gly Glu Gly Phe Gly Val Thr Gly
305 310 315 320
cct gtt gct ggt cgt tct ggt caa tgg att gct gaa caa gct ggt gtt 1008
Pro Val Ala Gly Arg Ser Gly Gln Trp Ile Ala Glu Gln Ala Gly Val
325 330 335
aac gtc cct aaa gat aaa gat gtt ctt ctt ttt gaa ctt gat aag aaa 1056
Asn Val Pro Lys Asp Lys Asp Val Leu Leu Phe Glu Leu Asp Lys Lys
340 345 350
aat att ggg gaa gct ctt tct tct gaa aaa ctt tct cct ttg ctt tca 1104
Asn Ile Gly Glu Ala Leu Ser Ser Glu Lys Leu Ser Pro Leu Leu Ser
355 360 365
atc tac aaa tca gaa aca cgt gaa gaa gga att gaa att gta cgt agc 1152
Ile Tyr Lys Ser Glu Thr Arg Glu Glu Gly Ile Glu Ile Val Arg Ser
370 375 380
tta ctt gct tac caa gga gct ggt cac aac gct gcc att caa atc ggt 1200
Leu Leu Ala Tyr Gln Gly Ala Gly His Asn Ala Ala Ile Gln Ile Gly
385 390 395 400
gca atg gac gac cca ttt gtc aaa gaa tac gga att aaa gtc gaa gct 1248
Ala Met Asp Asp Pro Phe Val Lys Glu Tyr Gly Ile Lys Val Glu Ala
405 410 415
tct cgt atc ctc gtt aac caa cct gac tct atc ggt ggg gtc gga gat 1296
Ser Arg Ile Leu Val Asn Gln Pro Asp Ser Ile Gly Gly Val Gly Asp
420 425 430
att tat act gat gca atg cgt cca tca ttg acg ctc gga act ggt tca 1344
Ile Tyr Thr Asp Ala Met Arg Pro Ser Leu Thr Leu Gly Thr Gly Ser
435 440 445
tgg ggg aaa aat tca ctt tca cac aat ttg agt aca tac gat cta ttg 1392
Trp Gly Lys Asn Ser Leu Ser His Asn Leu Ser Thr Tyr Asp Leu Leu
450 455 460
aat gtt aaa aca gtg gct aaa cgt cgt aat cgc cct caa tgg gtt cgt 1440
Asn Val Lys Thr Val Ala Lys Arg Arg Asn Arg Pro Gln Trp Val Arg
465 470 475 480
ttg cca aaa gaa att tac tac gaa aaa aat gca att tct tac tta caa 1488
Leu Pro Lys Glu Ile Tyr Tyr Glu Lys Asn Ala Ile Ser Tyr Leu Gln
485 490 495
gaa ttg cca cac gtc cac aaa gct ttc att gtt gcc gac cct ggt atg 1536
Glu Leu Pro His Val His Lys Ala Phe Ile Val Ala Asp Pro Gly Met
500 505 510
gtt aaa ttc ggt ttc gtt gat aaa gtt ttg gaa caa ctt gct atc cgc 1584
Val Lys Phe Gly Phe Val Asp Lys Val Leu Glu Gln Leu Ala Ile Arg
515 520 525
cca act caa gtt gaa aca agc att tat ggc tca gtc caa cct gac cca 1632
Pro Thr Gln Val Glu Thr Ser Ile Tyr Gly Ser Val Gln Pro Asp Pro
530 535 540
act ttg agt gaa gcc att gca atc gct cgt caa atg aaa caa ttt gaa 1680
Thr Leu Ser Glu Ala Ile Ala Ile Ala Arg Gln Met Lys Gln Phe Glu
545 550 555 560
cct gac act gtc atc tgt ctt ggt ggt ggt tct gct ctc gat gct ggt 1728
Pro Asp Thr Val Ile Cys Leu Gly Gly Gly Ser Ala Leu Asp Ala Gly
565 570 575
aag att ggt cgt ttg att tat gaa tat gat gct cgt ggt gag gct gac 1776
Lys Ile Gly Arg Leu Ile Tyr Glu Tyr Asp Ala Arg Gly Glu Ala Asp
580 585 590
ctt tcc gat gac gca agt ttg aaa gag atc ttc caa gag tta gct caa 1824
Leu Ser Asp Asp Ala Ser Leu Lys Glu Ile Phe Gln Glu Leu Ala Gln
595 600 605
aaa ttt gtt gat att cgt aaa cgt att atc aaa ttc tac cac cca cac 1872
Lys Phe Val Asp Ile Arg Lys Arg Ile Ile Lys Phe Tyr His Pro His
610 615 620
aaa gca caa atg gtt gct atc cct act act tct ggt act ggt tct gaa 1920
Lys Ala Gln Met Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu
625 630 635 640
gtg act cca ttt gcg gtt atc act gat gat gaa act cac gtt aaa tat 1968
Val Thr Pro Phe Ala Val Ile Thr Asp Asp Glu Thr His Val Lys Tyr
645 650 655
cca ctt gct gac tat caa ttg aca cct caa gtt gcc att gtt gac cct 2016
Pro Leu Ala Asp Tyr Gln Leu Thr Pro Gln Val Ala Ile Val Asp Pro
660 665 670
gag ttt gtt atg act gta cca aaa cgt act gtt tct tgg tct ggg att 2064
Glu Phe Val Met Thr Val Pro Lys Arg Thr Val Ser Trp Ser Gly Ile
675 680 685
gat gct atg tca cac gcg ctt gaa tct tat gtt tct gtc atg tct tct 2112
Asp Ala Met Ser His Ala Leu Glu Ser Tyr Val Ser Val Met Ser Ser
690 695 700
gac tat aca aaa cca att tca ctt caa gcc atc aaa ctc atc ttt gaa 2160
Asp Tyr Thr Lys Pro Ile Ser Leu Gln Ala Ile Lys Leu Ile Phe Glu
705 710 715 720
aac ttg act gag tct tat cat tat gac cca gct cat cca acc aaa gaa 2208
Asn Leu Thr Glu Ser Tyr His Tyr Asp Pro Ala His Pro Thr Lys Glu
725 730 735
ggt caa aaa gct cgc gaa aac atg cac aat gct gca aca ctc gct ggt 2256
Gly Gln Lys Ala Arg Glu Asn Met His Asn Ala Ala Thr Leu Ala Gly
740 745 750
atg gcc ttc gcc aat gct ttc ctt gga att aac cac tca ctt gct cat 2304
Met Ala Phe Ala Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His
755 760 765
aaa att ggt ggt gaa ttt ggg ctt cct cat ggt ctt gcc att gct atc 2352
Lys Ile Gly Gly Glu Phe Gly Leu Pro His Gly Leu Ala Ile Ala Ile
770 775 780
gct atg cca cat gtc att aaa ttt aac gct gta aca gga aac gtt aaa 2400
Ala Met Pro His Val Ile Lys Phe Asn Ala Val Thr Gly Asn Val Lys
785 790 795 800
cgt acc cct tac cca cgt tat gaa act tat cgt gcg caa gaa gac tac 2448
Arg Thr Pro Tyr Pro Arg Tyr Glu Thr Tyr Arg Ala Gln Glu Asp Tyr
805 810 815
gct gaa att tca cgc ttc atg gga ttt gct ggc aaa gaa gat tca gat 2496
Ala Glu Ile Ser Arg Phe Met Gly Phe Ala Gly Lys Glu Asp Ser Asp
820 825 830
gaa aaa gcg gtc aaa gct ttg gtt gct gaa ctt aaa aaa ttg act gat 2544
Glu Lys Ala Val Lys Ala Leu Val Ala Glu Leu Lys Lys Leu Thr Asp
835 840 845
agt att gat att aat atc acc ctt tca gga aat ggt gta gat aaa gct 2592
Ser Ile Asp Ile Asn Ile Thr Leu Ser Gly Asn Gly Val Asp Lys Ala
850 855 860
cat ctt gaa cgt gag ctt gat aaa ttg gct gac ctt gtt tac gat gac 2640
His Leu Glu Arg Glu Leu Asp Lys Leu Ala Asp Leu Val Tyr Asp Asp
865 870 875 880
caa tgt aca cct gct aat cca cgt caa cca aga att gat gag att aaa 2688
Gln Cys Thr Pro Ala Asn Pro Arg Gln Pro Arg Ile Asp Glu Ile Lys
885 890 895
caa ctc ttg tta gac caa tat taa 2712
Gln Leu Leu Leu Asp Gln Tyr
900
<210> 62
<211> 903
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 62
Met Ala Thr Lys Lys Ala Ala Pro Ala Ala Lys Lys Val Leu Ser Ala
1 5 10 15
Glu Glu Lys Ala Ala Lys Phe Gln Glu Ala Val Ala Tyr Thr Asp Gln
20 25 30
Leu Val Lys Lys Ala Gln Ala Ala Val Leu Lys Phe Glu Gly Tyr Thr
35 40 45
Gln Thr Gln Val Asp Thr Ile Val Ala Ala Met Ala Leu Ala Ala Ser
50 55 60
Lys His Ser Leu Glu Leu Ala His Glu Ala Val Asn Glu Thr Gly Arg
65 70 75 80
Gly Val Val Glu Asp Lys Asp Thr Lys Asn His Phe Ala Ser Glu Ser
85 90 95
Val Tyr Asn Ala Ile Lys Asn Asp Lys Thr Val Gly Val Ile Ala Glu
100 105 110
Asn Lys Val Ala Gly Ser Val Glu Ile Ala Ser Pro Leu Gly Val Leu
115 120 125
Ala Gly Ile Val Pro Thr Thr Asn Pro Thr Ser Thr Ala Ile Phe Lys
130 135 140
Ser Leu Leu Thr Ala Lys Thr Arg Asn Ala Ile Val Phe Ala Phe His
145 150 155 160
Pro Gln Ala Gln Lys Cys Ser Ser His Ala Ala Lys Ile Val Tyr Asp
165 170 175
Ala Ala Ile Glu Ala Gly Ala Pro Glu Asp Phe Ile Gln Trp Ile Glu
180 185 190
Val Pro Ser Leu Asp Met Thr Thr Ala Leu Ile Gln Asn Arg Gly Ile
195 200 205
Ala Thr Ile Leu Ala Thr Gly Gly Pro Gly Met Val Asn Ala Ala Leu
210 215 220
Lys Ser Gly Asn Pro Ser Leu Gly Val Gly Ala Gly Asn Gly Ala Val
225 230 235 240
Tyr Val Asp Ala Thr Ala Asn Ile Asp Arg Ala Val Glu Asp Leu Leu
245 250 255
Leu Ser Lys Arg Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser
260 265 270
Ala Val Ile Asp Ala Ser Ile Tyr Asp Glu Phe Val Ala Lys Met Gln
275 280 285
Ala Gln Gly Ala Tyr Met Val Pro Lys Lys Asp Tyr Lys Ala Ile Glu
290 295 300
Ser Phe Val Phe Val Glu Arg Ala Gly Glu Gly Phe Gly Val Thr Gly
305 310 315 320
Pro Val Ala Gly Arg Ser Gly Gln Trp Ile Ala Glu Gln Ala Gly Val
325 330 335
Asn Val Pro Lys Asp Lys Asp Val Leu Leu Phe Glu Leu Asp Lys Lys
340 345 350
Asn Ile Gly Glu Ala Leu Ser Ser Glu Lys Leu Ser Pro Leu Leu Ser
355 360 365
Ile Tyr Lys Ser Glu Thr Arg Glu Glu Gly Ile Glu Ile Val Arg Ser
370 375 380
Leu Leu Ala Tyr Gln Gly Ala Gly His Asn Ala Ala Ile Gln Ile Gly
385 390 395 400
Ala Met Asp Asp Pro Phe Val Lys Glu Tyr Gly Ile Lys Val Glu Ala
405 410 415
Ser Arg Ile Leu Val Asn Gln Pro Asp Ser Ile Gly Gly Val Gly Asp
420 425 430
Ile Tyr Thr Asp Ala Met Arg Pro Ser Leu Thr Leu Gly Thr Gly Ser
435 440 445
Trp Gly Lys Asn Ser Leu Ser His Asn Leu Ser Thr Tyr Asp Leu Leu
450 455 460
Asn Val Lys Thr Val Ala Lys Arg Arg Asn Arg Pro Gln Trp Val Arg
465 470 475 480
Leu Pro Lys Glu Ile Tyr Tyr Glu Lys Asn Ala Ile Ser Tyr Leu Gln
485 490 495
Glu Leu Pro His Val His Lys Ala Phe Ile Val Ala Asp Pro Gly Met
500 505 510
Val Lys Phe Gly Phe Val Asp Lys Val Leu Glu Gln Leu Ala Ile Arg
515 520 525
Pro Thr Gln Val Glu Thr Ser Ile Tyr Gly Ser Val Gln Pro Asp Pro
530 535 540
Thr Leu Ser Glu Ala Ile Ala Ile Ala Arg Gln Met Lys Gln Phe Glu
545 550 555 560
Pro Asp Thr Val Ile Cys Leu Gly Gly Gly Ser Ala Leu Asp Ala Gly
565 570 575
Lys Ile Gly Arg Leu Ile Tyr Glu Tyr Asp Ala Arg Gly Glu Ala Asp
580 585 590
Leu Ser Asp Asp Ala Ser Leu Lys Glu Ile Phe Gln Glu Leu Ala Gln
595 600 605
Lys Phe Val Asp Ile Arg Lys Arg Ile Ile Lys Phe Tyr His Pro His
610 615 620
Lys Ala Gln Met Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu
625 630 635 640
Val Thr Pro Phe Ala Val Ile Thr Asp Asp Glu Thr His Val Lys Tyr
645 650 655
Pro Leu Ala Asp Tyr Gln Leu Thr Pro Gln Val Ala Ile Val Asp Pro
660 665 670
Glu Phe Val Met Thr Val Pro Lys Arg Thr Val Ser Trp Ser Gly Ile
675 680 685
Asp Ala Met Ser His Ala Leu Glu Ser Tyr Val Ser Val Met Ser Ser
690 695 700
Asp Tyr Thr Lys Pro Ile Ser Leu Gln Ala Ile Lys Leu Ile Phe Glu
705 710 715 720
Asn Leu Thr Glu Ser Tyr His Tyr Asp Pro Ala His Pro Thr Lys Glu
725 730 735
Gly Gln Lys Ala Arg Glu Asn Met His Asn Ala Ala Thr Leu Ala Gly
740 745 750
Met Ala Phe Ala Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His
755 760 765
Lys Ile Gly Gly Glu Phe Gly Leu Pro His Gly Leu Ala Ile Ala Ile
770 775 780
Ala Met Pro His Val Ile Lys Phe Asn Ala Val Thr Gly Asn Val Lys
785 790 795 800
Arg Thr Pro Tyr Pro Arg Tyr Glu Thr Tyr Arg Ala Gln Glu Asp Tyr
805 810 815
Ala Glu Ile Ser Arg Phe Met Gly Phe Ala Gly Lys Glu Asp Ser Asp
820 825 830
Glu Lys Ala Val Lys Ala Leu Val Ala Glu Leu Lys Lys Leu Thr Asp
835 840 845
Ser Ile Asp Ile Asn Ile Thr Leu Ser Gly Asn Gly Val Asp Lys Ala
850 855 860
His Leu Glu Arg Glu Leu Asp Lys Leu Ala Asp Leu Val Tyr Asp Asp
865 870 875 880
Gln Cys Thr Pro Ala Asn Pro Arg Gln Pro Arg Ile Asp Glu Ile Lys
885 890 895
Gln Leu Leu Leu Asp Gln Tyr
900
<210> 63
<211> 2631
<212> DNA
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<220>
<221> CDS
<222> (1)..(2631)
<223> 嗜酸乳杆菌 NCFM; 基因 LBA0461 编码乙醇脱氢酶
<400> 63
atg gct aag gca caa caa aaa gaa act cct act gag gaa gaa aag aaa 48
Met Ala Lys Ala Gln Gln Lys Glu Thr Pro Thr Glu Glu Glu Lys Lys
1 5 10 15
aag caa atc tac gac atg gta gat aat tta gta aaa aga tca cat gtt 96
Lys Gln Ile Tyr Asp Met Val Asp Asn Leu Val Lys Arg Ser His Val
20 25 30
gca tta gat gaa atg gct aac ttc aca caa gaa caa gtt gat aag atc 144
Ala Leu Asp Glu Met Ala Asn Phe Thr Gln Glu Gln Val Asp Lys Ile
35 40 45
tgt gaa gca gtt gct act gca ggt gaa caa aac gct tac cca cta gct 192
Cys Glu Ala Val Ala Thr Ala Gly Glu Gln Asn Ala Tyr Pro Leu Ala
50 55 60
aaa atg gct gtt gaa gaa act aag cgt ggt gta gta gaa gat aag act 240
Lys Met Ala Val Glu Glu Thr Lys Arg Gly Val Val Glu Asp Lys Thr
65 70 75 80
act aaa aac atg tac gct agt gaa aat att tgg aac agt tta cgc cac 288
Thr Lys Asn Met Tyr Ala Ser Glu Asn Ile Trp Asn Ser Leu Arg His
85 90 95
gaa aaa act gtt ggt gta att gaa gaa gat aag gaa cta gga tta act 336
Glu Lys Thr Val Gly Val Ile Glu Glu Asp Lys Glu Leu Gly Leu Thr
100 105 110
aag att gct gaa cct aag ggt gtt att gcc ggt gtt act cca gta act 384
Lys Ile Ala Glu Pro Lys Gly Val Ile Ala Gly Val Thr Pro Val Thr
115 120 125
aac cca act tca act gtt att ttc aaa gca atg ctt gct cta aag acg 432
Asn Pro Thr Ser Thr Val Ile Phe Lys Ala Met Leu Ala Leu Lys Thr
130 135 140
aga aat act att att ttt ggt ttc cac cca caa gct caa aaa tgt tgt 480
Arg Asn Thr Ile Ile Phe Gly Phe His Pro Gln Ala Gln Lys Cys Cys
145 150 155 160
gta gaa act ggt aaa att atc caa gcg gct gct gtt gct gca ggt gca 528
Val Glu Thr Gly Lys Ile Ile Gln Ala Ala Ala Val Ala Ala Gly Ala
165 170 175
cct aaa gat gca att tta tgg att gaa gag cca agt ttg gat gca acc 576
Pro Lys Asp Ala Ile Leu Trp Ile Glu Glu Pro Ser Leu Asp Ala Thr
180 185 190
act gat tta atg aat aat cca ggt gtt caa act att ttg gcc act ggt 624
Thr Asp Leu Met Asn Asn Pro Gly Val Gln Thr Ile Leu Ala Thr Gly
195 200 205
ggt cca ggt atg gtt aag gct gca tat tca tca ggt aaa cca gct atc 672
Gly Pro Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile
210 215 220
ggt gtt ggt cct ggt aat ggt cct tca tac att gaa aaa tca gct aat 720
Gly Val Gly Pro Gly Asn Gly Pro Ser Tyr Ile Glu Lys Ser Ala Asn
225 230 235 240
atc ggt cgt gct gtt tat gat att gtt tta tca aag aac ttc gat aac 768
Ile Gly Arg Ala Val Tyr Asp Ile Val Leu Ser Lys Asn Phe Asp Asn
245 250 255
ggt atg gtt tgt gct tct gaa aac tca gtc gtt gta gat gac gca att 816
Gly Met Val Cys Ala Ser Glu Asn Ser Val Val Val Asp Asp Ala Ile
260 265 270
tat gac aag gct gta gaa gaa ttt aag aag tgg ggt tgt tac ttc tta 864
Tyr Asp Lys Ala Val Glu Glu Phe Lys Lys Trp Gly Cys Tyr Phe Leu
275 280 285
aat aaa gaa gaa att aag aaa ttt gaa gaa cac ttt att gat cca aga 912
Asn Lys Glu Glu Ile Lys Lys Phe Glu Glu His Phe Ile Asp Pro Arg
290 295 300
cgt ggt act gtt gca ggt cct gta gct ggt aaa tca gct tat gaa att 960
Arg Gly Thr Val Ala Gly Pro Val Ala Gly Lys Ser Ala Tyr Glu Ile
305 310 315 320
gct aaa ctt tgt ggt gtt gat gta cca aag act act aaa gta att gtt 1008
Ala Lys Leu Cys Gly Val Asp Val Pro Lys Thr Thr Lys Val Ile Val
325 330 335
gct gaa tac aaa ggt gtt ggt aga aag ttc cca ctt tca gct gaa aaa 1056
Ala Glu Tyr Lys Gly Val Gly Arg Lys Phe Pro Leu Ser Ala Glu Lys
340 345 350
ctt tct cct gtc ttt act tta tat cgc gct aag aat cat gac gat gca 1104
Leu Ser Pro Val Phe Thr Leu Tyr Arg Ala Lys Asn His Asp Asp Ala
355 360 365
ttt aag att tgt tct gaa ctt ctt gat tat ggt ggt cgt ggt cat acc 1152
Phe Lys Ile Cys Ser Glu Leu Leu Asp Tyr Gly Gly Arg Gly His Thr
370 375 380
gcg gga att cac tca agc gat caa aat ata att gat gaa ttt gca atg 1200
Ala Gly Ile His Ser Ser Asp Gln Asn Ile Ile Asp Glu Phe Ala Met
385 390 395 400
aag atg gat gct tgt cgt att cta gtt aat tca cca gct gca ctt ggt 1248
Lys Met Asp Ala Cys Arg Ile Leu Val Asn Ser Pro Ala Ala Leu Gly
405 410 415
ggt att ggt gat ctt tac aac aat atg ttg cct tca ctt aca tta ggt 1296
Gly Ile Gly Asp Leu Tyr Asn Asn Met Leu Pro Ser Leu Thr Leu Gly
420 425 430
aca ggt tca tac ggt gct aac aca ttt tca cac aat att ggt gcc aga 1344
Thr Gly Ser Tyr Gly Ala Asn Thr Phe Ser His Asn Ile Gly Ala Arg
435 440 445
gat tta ctt aat att aaa acg gtt gcg aaa cgt cgc gac aat atg caa 1392
Asp Leu Leu Asn Ile Lys Thr Val Ala Lys Arg Arg Asp Asn Met Gln
450 455 460
tgg gta aaa gtt cca tca aag act tac ttt gaa cat aat gct gct aat 1440
Trp Val Lys Val Pro Ser Lys Thr Tyr Phe Glu His Asn Ala Ala Asn
465 470 475 480
tat ttg cgt cac atg cca aat gtt aac cgt ttc ttc att gtt act gat 1488
Tyr Leu Arg His Met Pro Asn Val Asn Arg Phe Phe Ile Val Thr Asp
485 490 495
gaa ggc gta gct gac caa ggc ttt gct gac gaa atc act gat att att 1536
Glu Gly Val Ala Asp Gln Gly Phe Ala Asp Glu Ile Thr Asp Ile Ile
500 505 510
tct aag cgt cgt ggt caa aaa gaa tat gaa gta ttt aag gct gta acg 1584
Ser Lys Arg Arg Gly Gln Lys Glu Tyr Glu Val Phe Lys Ala Val Thr
515 520 525
ctt gat cct gat act gat gta att aaa gac ggt gtt cac aga atg aac 1632
Leu Asp Pro Asp Thr Asp Val Ile Lys Asp Gly Val His Arg Met Asn
530 535 540
atc ttt aag cct gac gta att att gct att ggt ggt ggc tca gta atg 1680
Ile Phe Lys Pro Asp Val Ile Ile Ala Ile Gly Gly Gly Ser Val Met
545 550 555 560
gat gct gct aaa gta atg cgc tta ttc tac gaa aat cca gaa atg agc 1728
Asp Ala Ala Lys Val Met Arg Leu Phe Tyr Glu Asn Pro Glu Met Ser
565 570 575
ttt gaa gaa gct tac caa aag ttt ttg gat atc aga aaa aga gta gtc 1776
Phe Glu Glu Ala Tyr Gln Lys Phe Leu Asp Ile Arg Lys Arg Val Val
580 585 590
cgc ttc cct aag att aat ggt gtt caa ctt gta tgt att cca aca act 1824
Arg Phe Pro Lys Ile Asn Gly Val Gln Leu Val Cys Ile Pro Thr Thr
595 600 605
tct ggt act ggt tca gaa gtt tcc cca att gca gtt att agc gat act 1872
Ser Gly Thr Gly Ser Glu Val Ser Pro Ile Ala Val Ile Ser Asp Thr
610 615 620
aaa aca ggt atg aaa cac act ctt tgt gat tat gct tta act cca gat 1920
Lys Thr Gly Met Lys His Thr Leu Cys Asp Tyr Ala Leu Thr Pro Asp
625 630 635 640
gtt tca att gtt gat gat caa ttt gtt caa aat atg cct aaa cgc tta 1968
Val Ser Ile Val Asp Asp Gln Phe Val Gln Asn Met Pro Lys Arg Leu
645 650 655
att gct tgg tca ggt ttt gaa gca tta ggt cat gct att gaa agc tat 2016
Ile Ala Trp Ser Gly Phe Glu Ala Leu Gly His Ala Ile Glu Ser Tyr
660 665 670
gtt tca act atg gcc act gat ttc act cgt ggt tgg tca ctt gaa gct 2064
Val Ser Thr Met Ala Thr Asp Phe Thr Arg Gly Trp Ser Leu Glu Ala
675 680 685
att aaa gcg atc ttt gca aac ttg aag aag tca tac gat ggt gat ctt 2112
Ile Lys Ala Ile Phe Ala Asn Leu Lys Lys Ser Tyr Asp Gly Asp Leu
690 695 700
gat gca aga aag caa atg cat gat gct gca act att gca ggt atg gca 2160
Asp Ala Arg Lys Gln Met His Asp Ala Ala Thr Ile Ala Gly Met Ala
705 710 715 720
tat tca aat gca ttc ctt ggt ctt gaa cac tca att gct cat act gtt 2208
Tyr Ser Asn Ala Phe Leu Gly Leu Glu His Ser Ile Ala His Thr Val
725 730 735
ggc tca acc ttt gat att cca agc gga gta agt gat gcg att gct tta 2256
Gly Ser Thr Phe Asp Ile Pro Ser Gly Val Ser Asp Ala Ile Ala Leu
740 745 750
ccg caa gtt att cgc ttt aat gcc aaa cgt cct gaa aag ctc gct atg 2304
Pro Gln Val Ile Arg Phe Asn Ala Lys Arg Pro Glu Lys Leu Ala Met
755 760 765
tgg cct cac tac tca gta tat cgt gct gaa agt gac tat gct gat att 2352
Trp Pro His Tyr Ser Val Tyr Arg Ala Glu Ser Asp Tyr Ala Asp Ile
770 775 780
gca aga gct atc ggt ttg act ggt tca agt gat gct gaa tta gtt gaa 2400
Ala Arg Ala Ile Gly Leu Thr Gly Ser Ser Asp Ala Glu Leu Val Glu
785 790 795 800
aaa ctg gtt caa aag att att gaa ttg gct cat tct gtt ggt att aag 2448
Lys Leu Val Gln Lys Ile Ile Glu Leu Ala His Ser Val Gly Ile Lys
805 810 815
atg gct tat aag gat tat ggc gtt gac aag act gaa ttt gat aag aaa 2496
Met Ala Tyr Lys Asp Tyr Gly Val Asp Lys Thr Glu Phe Asp Lys Lys
820 825 830
gtt gat caa atg gca gtt gaa gct tat ggt gat caa aat act gta act 2544
Val Asp Gln Met Ala Val Glu Ala Tyr Gly Asp Gln Asn Thr Val Thr
835 840 845
aat cca tca gca cca ctt att aca caa att gtt gtc tta atg aaa gat 2592
Asn Pro Ser Ala Pro Leu Ile Thr Gln Ile Val Val Leu Met Lys Asp
850 855 860
tgc tac gaa ggc aaa ggt att tca gac aat gat aaa taa 2631
Cys Tyr Glu Gly Lys Gly Ile Ser Asp Asn Asp Lys
865 870 875
<210> 64
<211> 876
<212> PRT
<213> 嗜酸乳杆菌（Lactobacillus acidophilus）
<400> 64
Met Ala Lys Ala Gln Gln Lys Glu Thr Pro Thr Glu Glu Glu Lys Lys
1 5 10 15
Lys Gln Ile Tyr Asp Met Val Asp Asn Leu Val Lys Arg Ser His Val
20 25 30
Ala Leu Asp Glu Met Ala Asn Phe Thr Gln Glu Gln Val Asp Lys Ile
35 40 45
Cys Glu Ala Val Ala Thr Ala Gly Glu Gln Asn Ala Tyr Pro Leu Ala
50 55 60
Lys Met Ala Val Glu Glu Thr Lys Arg Gly Val Val Glu Asp Lys Thr
65 70 75 80
Thr Lys Asn Met Tyr Ala Ser Glu Asn Ile Trp Asn Ser Leu Arg His
85 90 95
Glu Lys Thr Val Gly Val Ile Glu Glu Asp Lys Glu Leu Gly Leu Thr
100 105 110
Lys Ile Ala Glu Pro Lys Gly Val Ile Ala Gly Val Thr Pro Val Thr
115 120 125
Asn Pro Thr Ser Thr Val Ile Phe Lys Ala Met Leu Ala Leu Lys Thr
130 135 140
Arg Asn Thr Ile Ile Phe Gly Phe His Pro Gln Ala Gln Lys Cys Cys
145 150 155 160
Val Glu Thr Gly Lys Ile Ile Gln Ala Ala Ala Val Ala Ala Gly Ala
165 170 175
Pro Lys Asp Ala Ile Leu Trp Ile Glu Glu Pro Ser Leu Asp Ala Thr
180 185 190
Thr Asp Leu Met Asn Asn Pro Gly Val Gln Thr Ile Leu Ala Thr Gly
195 200 205
Gly Pro Gly Met Val Lys Ala Ala Tyr Ser Ser Gly Lys Pro Ala Ile
210 215 220
Gly Val Gly Pro Gly Asn Gly Pro Ser Tyr Ile Glu Lys Ser Ala Asn
225 230 235 240
Ile Gly Arg Ala Val Tyr Asp Ile Val Leu Ser Lys Asn Phe Asp Asn
245 250 255
Gly Met Val Cys Ala Ser Glu Asn Ser Val Val Val Asp Asp Ala Ile
260 265 270
Tyr Asp Lys Ala Val Glu Glu Phe Lys Lys Trp Gly Cys Tyr Phe Leu
275 280 285
Asn Lys Glu Glu Ile Lys Lys Phe Glu Glu His Phe Ile Asp Pro Arg
290 295 300
Arg Gly Thr Val Ala Gly Pro Val Ala Gly Lys Ser Ala Tyr Glu Ile
305 310 315 320
Ala Lys Leu Cys Gly Val Asp Val Pro Lys Thr Thr Lys Val Ile Val
325 330 335
Ala Glu Tyr Lys Gly Val Gly Arg Lys Phe Pro Leu Ser Ala Glu Lys
340 345 350
Leu Ser Pro Val Phe Thr Leu Tyr Arg Ala Lys Asn His Asp Asp Ala
355 360 365
Phe Lys Ile Cys Ser Glu Leu Leu Asp Tyr Gly Gly Arg Gly His Thr
370 375 380
Ala Gly Ile His Ser Ser Asp Gln Asn Ile Ile Asp Glu Phe Ala Met
385 390 395 400
Lys Met Asp Ala Cys Arg Ile Leu Val Asn Ser Pro Ala Ala Leu Gly
405 410 415
Gly Ile Gly Asp Leu Tyr Asn Asn Met Leu Pro Ser Leu Thr Leu Gly
420 425 430
Thr Gly Ser Tyr Gly Ala Asn Thr Phe Ser His Asn Ile Gly Ala Arg
435 440 445
Asp Leu Leu Asn Ile Lys Thr Val Ala Lys Arg Arg Asp Asn Met Gln
450 455 460
Trp Val Lys Val Pro Ser Lys Thr Tyr Phe Glu His Asn Ala Ala Asn
465 470 475 480
Tyr Leu Arg His Met Pro Asn Val Asn Arg Phe Phe Ile Val Thr Asp
485 490 495
Glu Gly Val Ala Asp Gln Gly Phe Ala Asp Glu Ile Thr Asp Ile Ile
500 505 510
Ser Lys Arg Arg Gly Gln Lys Glu Tyr Glu Val Phe Lys Ala Val Thr
515 520 525
Leu Asp Pro Asp Thr Asp Val Ile Lys Asp Gly Val His Arg Met Asn
530 535 540
Ile Phe Lys Pro Asp Val Ile Ile Ala Ile Gly Gly Gly Ser Val Met
545 550 555 560
Asp Ala Ala Lys Val Met Arg Leu Phe Tyr Glu Asn Pro Glu Met Ser
565 570 575
Phe Glu Glu Ala Tyr Gln Lys Phe Leu Asp Ile Arg Lys Arg Val Val
580 585 590
Arg Phe Pro Lys Ile Asn Gly Val Gln Leu Val Cys Ile Pro Thr Thr
595 600 605
Ser Gly Thr Gly Ser Glu Val Ser Pro Ile Ala Val Ile Ser Asp Thr
610 615 620
Lys Thr Gly Met Lys His Thr Leu Cys Asp Tyr Ala Leu Thr Pro Asp
625 630 635 640
Val Ser Ile Val Asp Asp Gln Phe Val Gln Asn Met Pro Lys Arg Leu
645 650 655
Ile Ala Trp Ser Gly Phe Glu Ala Leu Gly His Ala Ile Glu Ser Tyr
660 665 670
Val Ser Thr Met Ala Thr Asp Phe Thr Arg Gly Trp Ser Leu Glu Ala
675 680 685
Ile Lys Ala Ile Phe Ala Asn Leu Lys Lys Ser Tyr Asp Gly Asp Leu
690 695 700
Asp Ala Arg Lys Gln Met His Asp Ala Ala Thr Ile Ala Gly Met Ala
705 710 715 720
Tyr Ser Asn Ala Phe Leu Gly Leu Glu His Ser Ile Ala His Thr Val
725 730 735
Gly Ser Thr Phe Asp Ile Pro Ser Gly Val Ser Asp Ala Ile Ala Leu
740 745 750
Pro Gln Val Ile Arg Phe Asn Ala Lys Arg Pro Glu Lys Leu Ala Met
755 760 765
Trp Pro His Tyr Ser Val Tyr Arg Ala Glu Ser Asp Tyr Ala Asp Ile
770 775 780
Ala Arg Ala Ile Gly Leu Thr Gly Ser Ser Asp Ala Glu Leu Val Glu
785 790 795 800
Lys Leu Val Gln Lys Ile Ile Glu Leu Ala His Ser Val Gly Ile Lys
805 810 815
Met Ala Tyr Lys Asp Tyr Gly Val Asp Lys Thr Glu Phe Asp Lys Lys
820 825 830
Val Asp Gln Met Ala Val Glu Ala Tyr Gly Asp Gln Asn Thr Val Thr
835 840 845
Asn Pro Ser Ala Pro Leu Ile Thr Gln Ile Val Val Leu Met Lys Asp
850 855 860
Cys Tyr Glu Gly Lys Gly Ile Ser Asp Asn Asp Lys
865 870 875
<210> 65
<211> 2607
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(2607)
<223> 干酪乳杆菌 ATCC 334; 基因 LSEI_0775 编码乙醇脱氢酶
<400> 65
atg tta aag aat act gcg aag act gct acc gaa gtt gat gta aag agc 48
Met Leu Lys Asn Thr Ala Lys Thr Ala Thr Glu Val Asp Val Lys Ser
1 5 10 15
atg gtt gac gaa ctg gtt gcg aat gcg cat gcc gct ttg aag atc atg 96
Met Val Asp Glu Leu Val Ala Asn Ala His Ala Ala Leu Lys Ile Met
20 25 30
aaa act ttc gat caa gaa aag atc gat cac atc gtt cat cgg atg gca 144
Lys Thr Phe Asp Gln Glu Lys Ile Asp His Ile Val His Arg Met Ala
35 40 45
atc gct ggt ttg gat cat cat atg gaa ttg gca aaa ctc gct gtt gac 192
Ile Ala Gly Leu Asp His His Met Glu Leu Ala Lys Leu Ala Val Asp
50 55 60
gag acg ggc cgt ggc gtg tgg gaa gat aaa gcg atc aag aac atg ttc 240
Glu Thr Gly Arg Gly Val Trp Glu Asp Lys Ala Ile Lys Asn Met Phe
65 70 75 80
gcc acc gaa gaa att tgg cac tca atc aag aac aac aag acc gtt ggt 288
Ala Thr Glu Glu Ile Trp His Ser Ile Lys Asn Asn Lys Thr Val Gly
85 90 95
gtg atc aac gaa gac aaa caa cgc ggc ttg gta tcg att gct gaa cca 336
Val Ile Asn Glu Asp Lys Gln Arg Gly Leu Val Ser Ile Ala Glu Pro
100 105 110
att ggt gtc att gct ggt gtg acg cca gtg acg aac cca act tca acg 384
Ile Gly Val Ile Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr
115 120 125
acc atg ttc aaa tcc gaa att gcc att aaa acc cgt aat cca atc att 432
Thr Met Phe Lys Ser Glu Ile Ala Ile Lys Thr Arg Asn Pro Ile Ile
130 135 140
ttc gct ttc cat ccg ggt gcc caa aag tct tca gcg cgt gcg ttg gag 480
Phe Ala Phe His Pro Gly Ala Gln Lys Ser Ser Ala Arg Ala Leu Glu
145 150 155 160
gtc atc cgt gat gaa gcc gaa aaa gca ggc tta cca aag ggt gct ttg 528
Val Ile Arg Asp Glu Ala Glu Lys Ala Gly Leu Pro Lys Gly Ala Leu
165 170 175
caa tat atc cct gta cca agt atg gat gcg act aaa gct ttg atg gat 576
Gln Tyr Ile Pro Val Pro Ser Met Asp Ala Thr Lys Ala Leu Met Asp
180 185 190
cac cct ggt atc gcc acg att ttg gca acg ggc ggt cct ggc atg gtt 624
His Pro Gly Ile Ala Thr Ile Leu Ala Thr Gly Gly Pro Gly Met Val
195 200 205
aag tcc gcc tat tca tca ggc aag cca gca ctg ggt gtt ggg gcc ggg 672
Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Ala Gly
210 215 220
aat gcg cca gct tac att gaa gcc agc gct aac atc aaa caa gct gtc 720
Asn Ala Pro Ala Tyr Ile Glu Ala Ser Ala Asn Ile Lys Gln Ala Val
225 230 235 240
aat gac tta gta ctt tca aag agt ttt gat aat ggt atg att tgt gct 768
Asn Asp Leu Val Leu Ser Lys Ser Phe Asp Asn Gly Met Ile Cys Ala
245 250 255
tcc gaa caa ggc gtg att att gat tcc agc att tac gat gat gtg aag 816
Ser Glu Gln Gly Val Ile Ile Asp Ser Ser Ile Tyr Asp Asp Val Lys
260 265 270
aag gaa ttt gaa gcc caa ggt gct tac ttc gtt aaa cag aag gac atg 864
Lys Glu Phe Glu Ala Gln Gly Ala Tyr Phe Val Lys Gln Lys Asp Met
275 280 285
aag aag ttc gaa agt acc gtc att aat ctt gaa aag caa agt gtc aat 912
Lys Lys Phe Glu Ser Thr Val Ile Asn Leu Glu Lys Gln Ser Val Asn
290 295 300
ccg cga atc gtt ggt caa agt cct aag cag att gct gaa tgg gcg ggg 960
Pro Arg Ile Val Gly Gln Ser Pro Lys Gln Ile Ala Glu Trp Ala Gly
305 310 315 320
atc acg att cct gat aac acc acg att ctg att gct gaa ttg aaa ggt 1008
Ile Thr Ile Pro Asp Asn Thr Thr Ile Leu Ile Ala Glu Leu Lys Gly
325 330 335
gtt ggc gaa aag tat cca ctg tct cgt gaa aaa cta agt cca gta ctg 1056
Val Gly Glu Lys Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Val Leu
340 345 350
gcc atg gtc aaa gca gat ggt cat gaa gat gcc ttc aag aaa tgt gaa 1104
Ala Met Val Lys Ala Asp Gly His Glu Asp Ala Phe Lys Lys Cys Glu
355 360 365
acc atg ctt gat atc ggc ggt ttg ggt cat acg gca gtc att cac acg 1152
Thr Met Leu Asp Ile Gly Gly Leu Gly His Thr Ala Val Ile His Thr
370 375 380
gct gat gat gag tta gct ttg aag tat gcc gat gct atg caa gct tgc 1200
Ala Asp Asp Glu Leu Ala Leu Lys Tyr Ala Asp Ala Met Gln Ala Cys
385 390 395 400
cgg att ttg atc aac aca cca tct tct gtc ggc ggg att ggc gat ctt 1248
Arg Ile Leu Ile Asn Thr Pro Ser Ser Val Gly Gly Ile Gly Asp Leu
405 410 415
tac aac gaa atg att cca agt ttg aca ctg ggc tgc ggc tca tac ggt 1296
Tyr Asn Glu Met Ile Pro Ser Leu Thr Leu Gly Cys Gly Ser Tyr Gly
420 425 430
ggt aac tca att tcg cat aac gtg ggg acg gtc gac ttg ctt aac atc 1344
Gly Asn Ser Ile Ser His Asn Val Gly Thr Val Asp Leu Leu Asn Ile
435 440 445
aag aca atg gca aaa cgc cgc aat aac atg cag tgg atg aaa ttg cca 1392
Lys Thr Met Ala Lys Arg Arg Asn Asn Met Gln Trp Met Lys Leu Pro
450 455 460
cca aag atc tat ttt gaa aag aat tct gtt cgt tac ctc gaa cac atg 1440
Pro Lys Ile Tyr Phe Glu Lys Asn Ser Val Arg Tyr Leu Glu His Met
465 470 475 480
gaa ggc atc aaa cgg gcc ttc atc gtt gct gat cga agc atg gaa aag 1488
Glu Gly Ile Lys Arg Ala Phe Ile Val Ala Asp Arg Ser Met Glu Lys
485 490 495
tta ggc ttc gtc aag atc gtt gaa gat gtt ctg gct cgg cgt gaa aat 1536
Leu Gly Phe Val Lys Ile Val Glu Asp Val Leu Ala Arg Arg Glu Asn
500 505 510
cct gtt caa gtt cag act ttt gtc gat gtt gaa cct gat cca tca act 1584
Pro Val Gln Val Gln Thr Phe Val Asp Val Glu Pro Asp Pro Ser Thr
515 520 525
gac acg gtc ttc aag ggg acc gat atc atg cgc tca ttt gga ccg gat 1632
Asp Thr Val Phe Lys Gly Thr Asp Ile Met Arg Ser Phe Gly Pro Asp
530 535 540
acc gtc att gct atc ggc ggc ggg tct gtt atg gat gct gcc aaa ggg 1680
Thr Val Ile Ala Ile Gly Gly Gly Ser Val Met Asp Ala Ala Lys Gly
545 550 555 560
atg tgc ttg ttc aac gat gcc ggg gat gct gat ttc ttc ggc gct aag 1728
Met Cys Leu Phe Asn Asp Ala Gly Asp Ala Asp Phe Phe Gly Ala Lys
565 570 575
cag aaa ttc ttg gat atc cgg aag cgg act tac acc ttc ccg aat ctg 1776
Gln Lys Phe Leu Asp Ile Arg Lys Arg Thr Tyr Thr Phe Pro Asn Leu
580 585 590
aat aag aca aag ctt gtc tgc att cca aca act tct ggg act ggt tca 1824
Asn Lys Thr Lys Leu Val Cys Ile Pro Thr Thr Ser Gly Thr Gly Ser
595 600 605
gaa gta aca cct ttt gcg gtt atc acc gat tcc aag gct ggc atc aag 1872
Glu Val Thr Pro Phe Ala Val Ile Thr Asp Ser Lys Ala Gly Ile Lys
610 615 620
tat ccg tta gct gac tat gca ttg acg cca gac atc gcg att gtg gac 1920
Tyr Pro Leu Ala Asp Tyr Ala Leu Thr Pro Asp Ile Ala Ile Val Asp
625 630 635 640
agt cag ttc atc gaa tcc gta ccg cca gtg gtt gtc gct gat tcc ggt 1968
Ser Gln Phe Ile Glu Ser Val Pro Pro Val Val Val Ala Asp Ser Gly
645 650 655
ttg gat gtg ctc tgc cac gca acc gaa agt tat gtt tcg acc atg gcc 2016
Leu Asp Val Leu Cys His Ala Thr Glu Ser Tyr Val Ser Thr Met Ala
660 665 670
act cat tac acg aag ggc ctg agt ttg gaa gca atc aag ttg gtc ttc 2064
Thr His Tyr Thr Lys Gly Leu Ser Leu Glu Ala Ile Lys Leu Val Phe
675 680 685
gaa aat ctg gaa aag agt tat cat ggc gac gtc gaa gca aag tca aag 2112
Glu Asn Leu Glu Lys Ser Tyr His Gly Asp Val Glu Ala Lys Ser Lys
690 695 700
atg cat gat gct tca acc att gcc ggg atg gcc ttt gcc aac gca ctg 2160
Met His Asp Ala Ser Thr Ile Ala Gly Met Ala Phe Ala Asn Ala Leu
705 710 715 720
ttg ggc att aac cac agc att gcg cat aag att ggg caa gcg ttc cat 2208
Leu Gly Ile Asn His Ser Ile Ala His Lys Ile Gly Gln Ala Phe His
725 730 735
ctg cct cac ggt cgt tgc atc gcg atc acg atg cca cat gtt att cgc 2256
Leu Pro His Gly Arg Cys Ile Ala Ile Thr Met Pro His Val Ile Arg
740 745 750
ttc aat gcg agt caa ccg aag aag cgc gct atc tgg gcg aag tat agc 2304
Phe Asn Ala Ser Gln Pro Lys Lys Arg Ala Ile Trp Ala Lys Tyr Ser
755 760 765
tac ttc cgc gct aac gaa gac tac gca gaa atc gct cgc tac ctt ggc 2352
Tyr Phe Arg Ala Asn Glu Asp Tyr Ala Glu Ile Ala Arg Tyr Leu Gly
770 775 780
ctt cct ggc aag aca act gat gaa ttg gtt gaa agc tat gtc cag gcc 2400
Leu Pro Gly Lys Thr Thr Asp Glu Leu Val Glu Ser Tyr Val Gln Ala
785 790 795 800
ttc atc aag ctg gct cac agt gtt ggc gtc aag ctc agt ttg aag gaa 2448
Phe Ile Lys Leu Ala His Ser Val Gly Val Lys Leu Ser Leu Lys Glu
805 810 815
caa ggc gtt aaa aag gct gat ctc gac aag caa gtt gat cgt ctt gcc 2496
Gln Gly Val Lys Lys Ala Asp Leu Asp Lys Gln Val Asp Arg Leu Ala
820 825 830
gaa ctg gct tat gaa gac aac tgc acc gtt acc aac ccg caa gaa ccg 2544
Glu Leu Ala Tyr Glu Asp Asn Cys Thr Val Thr Asn Pro Gln Glu Pro
835 840 845
tta atc ggc gac ttg aag cat atc atc ctt gat gaa tat gaa ggc acc 2592
Leu Ile Gly Asp Leu Lys His Ile Ile Leu Asp Glu Tyr Glu Gly Thr
850 855 860
gaa tcc act ttc taa 2607
Glu Ser Thr Phe
865
<210> 66
<211> 868
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 66
Met Leu Lys Asn Thr Ala Lys Thr Ala Thr Glu Val Asp Val Lys Ser
1 5 10 15
Met Val Asp Glu Leu Val Ala Asn Ala His Ala Ala Leu Lys Ile Met
20 25 30
Lys Thr Phe Asp Gln Glu Lys Ile Asp His Ile Val His Arg Met Ala
35 40 45
Ile Ala Gly Leu Asp His His Met Glu Leu Ala Lys Leu Ala Val Asp
50 55 60
Glu Thr Gly Arg Gly Val Trp Glu Asp Lys Ala Ile Lys Asn Met Phe
65 70 75 80
Ala Thr Glu Glu Ile Trp His Ser Ile Lys Asn Asn Lys Thr Val Gly
85 90 95
Val Ile Asn Glu Asp Lys Gln Arg Gly Leu Val Ser Ile Ala Glu Pro
100 105 110
Ile Gly Val Ile Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr
115 120 125
Thr Met Phe Lys Ser Glu Ile Ala Ile Lys Thr Arg Asn Pro Ile Ile
130 135 140
Phe Ala Phe His Pro Gly Ala Gln Lys Ser Ser Ala Arg Ala Leu Glu
145 150 155 160
Val Ile Arg Asp Glu Ala Glu Lys Ala Gly Leu Pro Lys Gly Ala Leu
165 170 175
Gln Tyr Ile Pro Val Pro Ser Met Asp Ala Thr Lys Ala Leu Met Asp
180 185 190
His Pro Gly Ile Ala Thr Ile Leu Ala Thr Gly Gly Pro Gly Met Val
195 200 205
Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Ala Gly
210 215 220
Asn Ala Pro Ala Tyr Ile Glu Ala Ser Ala Asn Ile Lys Gln Ala Val
225 230 235 240
Asn Asp Leu Val Leu Ser Lys Ser Phe Asp Asn Gly Met Ile Cys Ala
245 250 255
Ser Glu Gln Gly Val Ile Ile Asp Ser Ser Ile Tyr Asp Asp Val Lys
260 265 270
Lys Glu Phe Glu Ala Gln Gly Ala Tyr Phe Val Lys Gln Lys Asp Met
275 280 285
Lys Lys Phe Glu Ser Thr Val Ile Asn Leu Glu Lys Gln Ser Val Asn
290 295 300
Pro Arg Ile Val Gly Gln Ser Pro Lys Gln Ile Ala Glu Trp Ala Gly
305 310 315 320
Ile Thr Ile Pro Asp Asn Thr Thr Ile Leu Ile Ala Glu Leu Lys Gly
325 330 335
Val Gly Glu Lys Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Val Leu
340 345 350
Ala Met Val Lys Ala Asp Gly His Glu Asp Ala Phe Lys Lys Cys Glu
355 360 365
Thr Met Leu Asp Ile Gly Gly Leu Gly His Thr Ala Val Ile His Thr
370 375 380
Ala Asp Asp Glu Leu Ala Leu Lys Tyr Ala Asp Ala Met Gln Ala Cys
385 390 395 400
Arg Ile Leu Ile Asn Thr Pro Ser Ser Val Gly Gly Ile Gly Asp Leu
405 410 415
Tyr Asn Glu Met Ile Pro Ser Leu Thr Leu Gly Cys Gly Ser Tyr Gly
420 425 430
Gly Asn Ser Ile Ser His Asn Val Gly Thr Val Asp Leu Leu Asn Ile
435 440 445
Lys Thr Met Ala Lys Arg Arg Asn Asn Met Gln Trp Met Lys Leu Pro
450 455 460
Pro Lys Ile Tyr Phe Glu Lys Asn Ser Val Arg Tyr Leu Glu His Met
465 470 475 480
Glu Gly Ile Lys Arg Ala Phe Ile Val Ala Asp Arg Ser Met Glu Lys
485 490 495
Leu Gly Phe Val Lys Ile Val Glu Asp Val Leu Ala Arg Arg Glu Asn
500 505 510
Pro Val Gln Val Gln Thr Phe Val Asp Val Glu Pro Asp Pro Ser Thr
515 520 525
Asp Thr Val Phe Lys Gly Thr Asp Ile Met Arg Ser Phe Gly Pro Asp
530 535 540
Thr Val Ile Ala Ile Gly Gly Gly Ser Val Met Asp Ala Ala Lys Gly
545 550 555 560
Met Cys Leu Phe Asn Asp Ala Gly Asp Ala Asp Phe Phe Gly Ala Lys
565 570 575
Gln Lys Phe Leu Asp Ile Arg Lys Arg Thr Tyr Thr Phe Pro Asn Leu
580 585 590
Asn Lys Thr Lys Leu Val Cys Ile Pro Thr Thr Ser Gly Thr Gly Ser
595 600 605
Glu Val Thr Pro Phe Ala Val Ile Thr Asp Ser Lys Ala Gly Ile Lys
610 615 620
Tyr Pro Leu Ala Asp Tyr Ala Leu Thr Pro Asp Ile Ala Ile Val Asp
625 630 635 640
Ser Gln Phe Ile Glu Ser Val Pro Pro Val Val Val Ala Asp Ser Gly
645 650 655
Leu Asp Val Leu Cys His Ala Thr Glu Ser Tyr Val Ser Thr Met Ala
660 665 670
Thr His Tyr Thr Lys Gly Leu Ser Leu Glu Ala Ile Lys Leu Val Phe
675 680 685
Glu Asn Leu Glu Lys Ser Tyr His Gly Asp Val Glu Ala Lys Ser Lys
690 695 700
Met His Asp Ala Ser Thr Ile Ala Gly Met Ala Phe Ala Asn Ala Leu
705 710 715 720
Leu Gly Ile Asn His Ser Ile Ala His Lys Ile Gly Gln Ala Phe His
725 730 735
Leu Pro His Gly Arg Cys Ile Ala Ile Thr Met Pro His Val Ile Arg
740 745 750
Phe Asn Ala Ser Gln Pro Lys Lys Arg Ala Ile Trp Ala Lys Tyr Ser
755 760 765
Tyr Phe Arg Ala Asn Glu Asp Tyr Ala Glu Ile Ala Arg Tyr Leu Gly
770 775 780
Leu Pro Gly Lys Thr Thr Asp Glu Leu Val Glu Ser Tyr Val Gln Ala
785 790 795 800
Phe Ile Lys Leu Ala His Ser Val Gly Val Lys Leu Ser Leu Lys Glu
805 810 815
Gln Gly Val Lys Lys Ala Asp Leu Asp Lys Gln Val Asp Arg Leu Ala
820 825 830
Glu Leu Ala Tyr Glu Asp Asn Cys Thr Val Thr Asn Pro Gln Glu Pro
835 840 845
Leu Ile Gly Asp Leu Lys His Ile Ile Leu Asp Glu Tyr Glu Gly Thr
850 855 860
Glu Ser Thr Phe
865
<210> 67
<211> 1194
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(1194)
<223> 干酪乳杆菌 ATCC 334; 基因 LSEI_2125 编码乙醇脱氢酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（TTG）处的tRNA插入Met而不是Leu
<400> 67
ttg aag aat ttt cga ttt cag aat aaa acc gat att cgt ttt ggt aca 48
Leu Lys Asn Phe Arg Phe Gln Asn Lys Thr Asp Ile Arg Phe Gly Thr
1 5 10 15
ggc cga tta gat gtt gag ctg cac gac gcg gtg agt caa ttt ggt tcg 96
Gly Arg Leu Asp Val Glu Leu His Asp Ala Val Ser Gln Phe Gly Ser
20 25 30
cgt gtt ttg ttc gtt tat ggt ggc cat tcc atc aaa aag tca ggc tta 144
Arg Val Leu Phe Val Tyr Gly Gly His Ser Ile Lys Lys Ser Gly Leu
35 40 45
tac gat acc gtg act gga ctg ctc gct gat ttg cag tta acc gaa ttg 192
Tyr Asp Thr Val Thr Gly Leu Leu Ala Asp Leu Gln Leu Thr Glu Leu
50 55 60
ccg ggc att gag ccg aac ccg aag atc gat tct atc cgt gct ggc caa 240
Pro Gly Ile Glu Pro Asn Pro Lys Ile Asp Ser Ile Arg Ala Gly Gln
65 70 75 80
aag ctg gcg aag gat cag gat att gat gtt gtt ttg gca gtt ggt ggc 288
Lys Leu Ala Lys Asp Gln Asp Ile Asp Val Val Leu Ala Val Gly Gly
85 90 95
ggc tcc gtc att gat gct gca aaa gtc att gcc agc gcc aag ttt tac 336
Gly Ser Val Ile Asp Ala Ala Lys Val Ile Ala Ser Ala Lys Phe Tyr
100 105 110
gaa ggc gac ccc tgg gat ctt gtc aaa gat cgt ggt gta act cgc cgg 384
Glu Gly Asp Pro Trp Asp Leu Val Lys Asp Arg Gly Val Thr Arg Arg
115 120 125
aaa ttg cag caa ttg cca gtc gtc gat att ctg aca tta gca gcg acc 432
Lys Leu Gln Gln Leu Pro Val Val Asp Ile Leu Thr Leu Ala Ala Thr
130 135 140
ggc agt gag atg aac agt ggc tcc gtg att tca aat cct gtc act cat 480
Gly Ser Glu Met Asn Ser Gly Ser Val Ile Ser Asn Pro Val Thr His
145 150 155 160
gaa aag ctc ggc aca gtc ggg cca aac aca cca gcg gtt tca ttt ttg 528
Glu Lys Leu Gly Thr Val Gly Pro Asn Thr Pro Ala Val Ser Phe Leu
165 170 175
gat ccg tca ctg acc ttt act gtg cca gcc aga caa acg gcg gcg gga 576
Asp Pro Ser Leu Thr Phe Thr Val Pro Ala Arg Gln Thr Ala Ala Gly
180 185 190
tca atg gat att ctt agt cat ttg ctt gaa caa tat ttt gat cgt tca 624
Ser Met Asp Ile Leu Ser His Leu Leu Glu Gln Tyr Phe Asp Arg Ser
195 200 205
aaa aat aat gac gcc agc aaa ggc atg att gaa ggc ttg atg cgc aca 672
Lys Asn Asn Asp Ala Ser Lys Gly Met Ile Glu Gly Leu Met Arg Thr
210 215 220
gtt att aag tgg gcg ccc att gcg ttg aaa caa ccc gat aac tat gat 720
Val Ile Lys Trp Ala Pro Ile Ala Leu Lys Gln Pro Asp Asn Tyr Asp
225 230 235 240
gcc cgc gcc aac tta atg tgg agc gca acg atg gcg cta aac ggt atc 768
Ala Arg Ala Asn Leu Met Trp Ser Ala Thr Met Ala Leu Asn Gly Ile
245 250 255
gtg agt gtt gct aac gaa aat ggc tgg aca gtg cat agc ctt gaa cat 816
Val Ser Val Ala Asn Glu Asn Gly Trp Thr Val His Ser Leu Glu His
260 265 270
gaa cta tca gct tat tat gac att acc cat ggt gtc ggc ctt ggc att 864
Glu Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Gly Ile
275 280 285
tta aca ccg cgt tgg atg tcg ttt att ctg acc aag gac gca acg aca 912
Leu Thr Pro Arg Trp Met Ser Phe Ile Leu Thr Lys Asp Ala Thr Thr
290 295 300
gca gaa atg ttg gca caa ttt ggt cgc cat gtc tgg gat ctt gaa ggt 960
Ala Glu Met Leu Ala Gln Phe Gly Arg His Val Trp Asp Leu Glu Gly
305 310 315 320
gat gaa tct tat gcc gtg gcg caa gcg gct att gac gcg acc ttt gat 1008
Asp Glu Ser Tyr Ala Val Ala Gln Ala Ala Ile Asp Ala Thr Phe Asp
325 330 335
tgg att agt cgc tta ggg ttc ccg atg acc ttg cct gaa gtt ggg atc 1056
Trp Ile Ser Arg Leu Gly Phe Pro Met Thr Leu Pro Glu Val Gly Ile
340 345 350
aag gat act cag cat ttt gat gcc atg gcg act gct gct gtt gcc aat 1104
Lys Asp Thr Gln His Phe Asp Ala Met Ala Thr Ala Ala Val Ala Asn
355 360 365
gct agt ctt gaa acg cgc gcc tat gtg ccg ttg tcg gta gaa gat gtc 1152
Ala Ser Leu Glu Thr Arg Ala Tyr Val Pro Leu Ser Val Glu Asp Val
370 375 380
gaa acg att tat caa gca tcc atg aca gat ggg ctg gca taa 1194
Glu Thr Ile Tyr Gln Ala Ser Met Thr Asp Gly Leu Ala
385 390 395
<210> 68
<211> 397
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 68
Leu Lys Asn Phe Arg Phe Gln Asn Lys Thr Asp Ile Arg Phe Gly Thr
1 5 10 15
Gly Arg Leu Asp Val Glu Leu His Asp Ala Val Ser Gln Phe Gly Ser
20 25 30
Arg Val Leu Phe Val Tyr Gly Gly His Ser Ile Lys Lys Ser Gly Leu
35 40 45
Tyr Asp Thr Val Thr Gly Leu Leu Ala Asp Leu Gln Leu Thr Glu Leu
50 55 60
Pro Gly Ile Glu Pro Asn Pro Lys Ile Asp Ser Ile Arg Ala Gly Gln
65 70 75 80
Lys Leu Ala Lys Asp Gln Asp Ile Asp Val Val Leu Ala Val Gly Gly
85 90 95
Gly Ser Val Ile Asp Ala Ala Lys Val Ile Ala Ser Ala Lys Phe Tyr
100 105 110
Glu Gly Asp Pro Trp Asp Leu Val Lys Asp Arg Gly Val Thr Arg Arg
115 120 125
Lys Leu Gln Gln Leu Pro Val Val Asp Ile Leu Thr Leu Ala Ala Thr
130 135 140
Gly Ser Glu Met Asn Ser Gly Ser Val Ile Ser Asn Pro Val Thr His
145 150 155 160
Glu Lys Leu Gly Thr Val Gly Pro Asn Thr Pro Ala Val Ser Phe Leu
165 170 175
Asp Pro Ser Leu Thr Phe Thr Val Pro Ala Arg Gln Thr Ala Ala Gly
180 185 190
Ser Met Asp Ile Leu Ser His Leu Leu Glu Gln Tyr Phe Asp Arg Ser
195 200 205
Lys Asn Asn Asp Ala Ser Lys Gly Met Ile Glu Gly Leu Met Arg Thr
210 215 220
Val Ile Lys Trp Ala Pro Ile Ala Leu Lys Gln Pro Asp Asn Tyr Asp
225 230 235 240
Ala Arg Ala Asn Leu Met Trp Ser Ala Thr Met Ala Leu Asn Gly Ile
245 250 255
Val Ser Val Ala Asn Glu Asn Gly Trp Thr Val His Ser Leu Glu His
260 265 270
Glu Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Gly Ile
275 280 285
Leu Thr Pro Arg Trp Met Ser Phe Ile Leu Thr Lys Asp Ala Thr Thr
290 295 300
Ala Glu Met Leu Ala Gln Phe Gly Arg His Val Trp Asp Leu Glu Gly
305 310 315 320
Asp Glu Ser Tyr Ala Val Ala Gln Ala Ala Ile Asp Ala Thr Phe Asp
325 330 335
Trp Ile Ser Arg Leu Gly Phe Pro Met Thr Leu Pro Glu Val Gly Ile
340 345 350
Lys Asp Thr Gln His Phe Asp Ala Met Ala Thr Ala Ala Val Ala Asn
355 360 365
Ala Ser Leu Glu Thr Arg Ala Tyr Val Pro Leu Ser Val Glu Asp Val
370 375 380
Glu Thr Ile Tyr Gln Ala Ser Met Thr Asp Gly Leu Ala
385 390 395
<210> 69
<211> 2604
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(2604)
<223> 植物乳杆菌 WCFS1; 基因 lp_3662编码乙醇脱氢酶
<400> 69
atg att aag aca gaa aaa aat caa aca agc aaa gtt act gac gaa gtt 48
Met Ile Lys Thr Glu Lys Asn Gln Thr Ser Lys Val Thr Asp Glu Val
1 5 10 15
gat caa tta gtt caa cgt tct aag aaa gca tta gct att ttg aag tca 96
Asp Gln Leu Val Gln Arg Ser Lys Lys Ala Leu Ala Ile Leu Lys Ser
20 25 30
tat acc caa gca caa atc gat gat tta tgt gaa aag gtt gcg gtt gcc 144
Tyr Thr Gln Ala Gln Ile Asp Asp Leu Cys Glu Lys Val Ala Val Ala
35 40 45
gca ctg gac aat cat atg aag tta gcc aag ttg gcg gtt gag gaa acc 192
Ala Leu Asp Asn His Met Lys Leu Ala Lys Leu Ala Val Glu Glu Thr
50 55 60
ggt cgg ggt gtt gtt gaa gat aag gcg att aag aac atc tac gcc agc 240
Gly Arg Gly Val Val Glu Asp Lys Ala Ile Lys Asn Ile Tyr Ala Ser
65 70 75 80
gaa tat atc tgg aac agc atg cgc cat gat aag aca gtc ggc gtc att 288
Glu Tyr Ile Trp Asn Ser Met Arg His Asp Lys Thr Val Gly Val Ile
85 90 95
aaa gaa gac gat gaa gag caa cta atg gaa att gct gaa cca gtt ggt 336
Lys Glu Asp Asp Glu Glu Gln Leu Met Glu Ile Ala Glu Pro Val Gly
100 105 110
atc gta gcc ggg gtt aca ccc gta acg aat cca act tca aca acg gtt 384
Ile Val Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Val
115 120 125
ttc aag acg ttg atc tca tta aag ggg cgt aac acg atc gtc ttt ggt 432
Phe Lys Thr Leu Ile Ser Leu Lys Gly Arg Asn Thr Ile Val Phe Gly
130 135 140
ttc cat cct caa gct cag aaa tgt agt tct gcg gct gct gat gtg atg 480
Phe His Pro Gln Ala Gln Lys Cys Ser Ser Ala Ala Ala Asp Val Met
145 150 155 160
cgg gaa gcg att aaa gct gct ggt ggt cca gct gat gct gtt tta tac 528
Arg Glu Ala Ile Lys Ala Ala Gly Gly Pro Ala Asp Ala Val Leu Tyr
165 170 175
atc gaa cac cca agc att gaa gca acc gac gcc ttg atg cac cat acg 576
Ile Glu His Pro Ser Ile Glu Ala Thr Asp Ala Leu Met His His Thr
180 185 190
gat gtt gcg acg atc ttg gct acc ggt ggt cca agt atg gtg aca gcc 624
Asp Val Ala Thr Ile Leu Ala Thr Gly Gly Pro Ser Met Val Thr Ala
195 200 205
gct tac tca tca ggt aaa cca gca ctg ggt gtt ggt cct ggt aac ggc 672
Ala Tyr Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Gly
210 215 220
cca act tat gtt gaa aaa aca gct gac att aag cag gcg gtt aac gac 720
Pro Thr Tyr Val Glu Lys Thr Ala Asp Ile Lys Gln Ala Val Asn Asp
225 230 235 240
att gtt tta tcg aag acg ttt gat aat ggg atg att tgt gct tct gaa 768
Ile Val Leu Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala Ser Glu
245 250 255
aat agt gcc att atc gac aag gaa att tat gct gaa gtt aaa gct gaa 816
Asn Ser Ala Ile Ile Asp Lys Glu Ile Tyr Ala Glu Val Lys Ala Glu
260 265 270
ttt att cgt ttg ggt tgc tac tac gtt aaa cct aaa gat gtg caa gcc 864
Phe Ile Arg Leu Gly Cys Tyr Tyr Val Lys Pro Lys Asp Val Gln Ala
275 280 285
ttg agt gat gct gtc att gat cct aac cgg cac aca gtt cgt ggt cca 912
Leu Ser Asp Ala Val Ile Asp Pro Asn Arg His Thr Val Arg Gly Pro
290 295 300
gtt gct ggt aaa aca gct tat caa atc gca caa atg gct ggc ttg aaa 960
Val Ala Gly Lys Thr Ala Tyr Gln Ile Ala Gln Met Ala Gly Leu Lys
305 310 315 320
gat gtc gct aag gac tgc cgg gtc ttg att gcg gag att aat ggt gtc 1008
Asp Val Ala Lys Asp Cys Arg Val Leu Ile Ala Glu Ile Asn Gly Val
325 330 335
ggt atc aag tac cca tta tcc ggc gaa aaa tta tca cca gtt ctg acc 1056
Gly Ile Lys Tyr Pro Leu Ser Gly Glu Lys Leu Ser Pro Val Leu Thr
340 345 350
gtt tac aaa gcg gat tcg cat gaa gcg gcg ttc aaa cgg gct aat gaa 1104
Val Tyr Lys Ala Asp Ser His Glu Ala Ala Phe Lys Arg Ala Asn Glu
355 360 365
tta ctt cac tat ggt ggc tta ggc cat act gct ggg att cat acg acc 1152
Leu Leu His Tyr Gly Gly Leu Gly His Thr Ala Gly Ile His Thr Thr
370 375 380
gat gat gca ttg gtc aaa gaa ttt ggc tta caa atg cca gct tgc cgg 1200
Asp Asp Ala Leu Val Lys Glu Phe Gly Leu Gln Met Pro Ala Cys Arg
385 390 395 400
atc tta gtt aac acg cca tct tca gtt ggt ggg tta ggt aac atc tac 1248
Ile Leu Val Asn Thr Pro Ser Ser Val Gly Gly Leu Gly Asn Ile Tyr
405 410 415
aat aac atg gcg cct tca tta act ctg ggc acg gga tca tat ggt ggc 1296
Asn Asn Met Ala Pro Ser Leu Thr Leu Gly Thr Gly Ser Tyr Gly Gly
420 425 430
aac tcg att tct cat aac gtt aca gat atg gat ctg att aat atc aaa 1344
Asn Ser Ile Ser His Asn Val Thr Asp Met Asp Leu Ile Asn Ile Lys
435 440 445
act gtg gca aaa cgg cgt aat aac atg caa tgg gtc aaa atg cct ccc 1392
Thr Val Ala Lys Arg Arg Asn Asn Met Gln Trp Val Lys Met Pro Pro
450 455 460
aaa gtc tac ttc gaa cgc aat tcc gtt cgc tac ttg gaa cac atg gcc 1440
Lys Val Tyr Phe Glu Arg Asn Ser Val Arg Tyr Leu Glu His Met Ala
465 470 475 480
ggg att aag aag gtt ttc cta gtt tgt gat cct ggg atg gtt gaa ttt 1488
Gly Ile Lys Lys Val Phe Leu Val Cys Asp Pro Gly Met Val Glu Phe
485 490 495
ggc tat gct gat cga gta acg gcg gtc ttg aac aag cgg act gat ccg 1536
Gly Tyr Ala Asp Arg Val Thr Ala Val Leu Asn Lys Arg Thr Asp Pro
500 505 510
gtt gat atc gac atc ttc tca gaa gtt gaa ccg aac ccg tcg act gac 1584
Val Asp Ile Asp Ile Phe Ser Glu Val Glu Pro Asn Pro Ser Thr Asp
515 520 525
aca gtc tac aaa ggg gtt gcc cgg atg aag gcc ttt aag cca gat aca 1632
Thr Val Tyr Lys Gly Val Ala Arg Met Lys Ala Phe Lys Pro Asp Thr
530 535 540
atc att gca ctc ggt ggt ggt tct gca atg gac gcc gct aag ggg atg 1680
Ile Ile Ala Leu Gly Gly Gly Ser Ala Met Asp Ala Ala Lys Gly Met
545 550 555 560
tgg ctc ttc tat gaa cac cca gaa gct tcc ttc tta ggt gct aaa cag 1728
Trp Leu Phe Tyr Glu His Pro Glu Ala Ser Phe Leu Gly Ala Lys Gln
565 570 575
aag ttc ctg gat atc cgg aag cgg acg tac aaa gta cca gta tcc gaa 1776
Lys Phe Leu Asp Ile Arg Lys Arg Thr Tyr Lys Val Pro Val Ser Glu
580 585 590
aaa gta act tat att ggg att cca acg act tct ggg act ggt tct gaa 1824
Lys Val Thr Tyr Ile Gly Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu
595 600 605
gta acg cca tac gcg gtt att acg gac tcc aag acg cac gtc aaa tac 1872
Val Thr Pro Tyr Ala Val Ile Thr Asp Ser Lys Thr His Val Lys Tyr
610 615 620
ccg att acg gac tac gca atg caa cca gat att gcg atc gtt gac cca 1920
Pro Ile Thr Asp Tyr Ala Met Gln Pro Asp Ile Ala Ile Val Asp Pro
625 630 635 640
caa ttt gtt gaa acg gtg cca aag cgg acg acg gct tgg act ggt ctc 1968
Gln Phe Val Glu Thr Val Pro Lys Arg Thr Thr Ala Trp Thr Gly Leu
645 650 655
gac gta att acc cac gca acc gaa gca tat gtt tca acg atg gct tct 2016
Asp Val Ile Thr His Ala Thr Glu Ala Tyr Val Ser Thr Met Ala Ser
660 665 670
gac ttc aca cgc ggc tgg tca att caa gcc ttg caa tta gcc ttc aag 2064
Asp Phe Thr Arg Gly Trp Ser Ile Gln Ala Leu Gln Leu Ala Phe Lys
675 680 685
tac ttg aaa gct tct tat gat ggt gac aag atg gct cgt gag aag atg 2112
Tyr Leu Lys Ala Ser Tyr Asp Gly Asp Lys Met Ala Arg Glu Lys Met
690 695 700
cac aat gcc tca acg tta gct ggg atg gcc ttt gcc aat gcc ttc ttg 2160
His Asn Ala Ser Thr Leu Ala Gly Met Ala Phe Ala Asn Ala Phe Leu
705 710 715 720
ggt atc aac cac tcg atc gcc cat aaa tta ggc ggg gaa ttc aac tta 2208
Gly Ile Asn His Ser Ile Ala His Lys Leu Gly Gly Glu Phe Asn Leu
725 730 735
cca cac gga ttg gca att gcg atc act tat ccg caa gtc gtt cgt tac 2256
Pro His Gly Leu Ala Ile Ala Ile Thr Tyr Pro Gln Val Val Arg Tyr
740 745 750
aat gct gaa atc cca acg aag tta gca atg tgg ccg aag tac aac cat 2304
Asn Ala Glu Ile Pro Thr Lys Leu Ala Met Trp Pro Lys Tyr Asn His
755 760 765
aat act gcc tta gcc gac tat gcc aac att gcc cgg gcc tta ggg tta 2352
Asn Thr Ala Leu Ala Asp Tyr Ala Asn Ile Ala Arg Ala Leu Gly Leu
770 775 780
cca ggc aag acc gac gaa gaa ctc aaa gaa agc tta gtt aaa gct tac 2400
Pro Gly Lys Thr Asp Glu Glu Leu Lys Glu Ser Leu Val Lys Ala Tyr
785 790 795 800
att gac ttg gct cac tca atg gat gtg acc tta tcc ttg aaa gct aac 2448
Ile Asp Leu Ala His Ser Met Asp Val Thr Leu Ser Leu Lys Ala Asn
805 810 815
cgg gtc gaa aag aag cac ttt gac gcc acc gtc gac gaa tta gcc gaa 2496
Arg Val Glu Lys Lys His Phe Asp Ala Thr Val Asp Glu Leu Ala Glu
820 825 830
ctg gcc tat gaa gac caa tgc acg acc gct aac cca cgc gaa cct tta 2544
Leu Ala Tyr Glu Asp Gln Cys Thr Thr Ala Asn Pro Arg Glu Pro Leu
835 840 845
atc agt gaa tta aag gcg att att gaa cgt gaa tgg gac ggc caa ggt 2592
Ile Ser Glu Leu Lys Ala Ile Ile Glu Arg Glu Trp Asp Gly Gln Gly
850 855 860
act gaa aaa tag 2604
Thr Glu Lys
865
<210> 70
<211> 867
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 70
Met Ile Lys Thr Glu Lys Asn Gln Thr Ser Lys Val Thr Asp Glu Val
1 5 10 15
Asp Gln Leu Val Gln Arg Ser Lys Lys Ala Leu Ala Ile Leu Lys Ser
20 25 30
Tyr Thr Gln Ala Gln Ile Asp Asp Leu Cys Glu Lys Val Ala Val Ala
35 40 45
Ala Leu Asp Asn His Met Lys Leu Ala Lys Leu Ala Val Glu Glu Thr
50 55 60
Gly Arg Gly Val Val Glu Asp Lys Ala Ile Lys Asn Ile Tyr Ala Ser
65 70 75 80
Glu Tyr Ile Trp Asn Ser Met Arg His Asp Lys Thr Val Gly Val Ile
85 90 95
Lys Glu Asp Asp Glu Glu Gln Leu Met Glu Ile Ala Glu Pro Val Gly
100 105 110
Ile Val Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Val
115 120 125
Phe Lys Thr Leu Ile Ser Leu Lys Gly Arg Asn Thr Ile Val Phe Gly
130 135 140
Phe His Pro Gln Ala Gln Lys Cys Ser Ser Ala Ala Ala Asp Val Met
145 150 155 160
Arg Glu Ala Ile Lys Ala Ala Gly Gly Pro Ala Asp Ala Val Leu Tyr
165 170 175
Ile Glu His Pro Ser Ile Glu Ala Thr Asp Ala Leu Met His His Thr
180 185 190
Asp Val Ala Thr Ile Leu Ala Thr Gly Gly Pro Ser Met Val Thr Ala
195 200 205
Ala Tyr Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Gly
210 215 220
Pro Thr Tyr Val Glu Lys Thr Ala Asp Ile Lys Gln Ala Val Asn Asp
225 230 235 240
Ile Val Leu Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala Ser Glu
245 250 255
Asn Ser Ala Ile Ile Asp Lys Glu Ile Tyr Ala Glu Val Lys Ala Glu
260 265 270
Phe Ile Arg Leu Gly Cys Tyr Tyr Val Lys Pro Lys Asp Val Gln Ala
275 280 285
Leu Ser Asp Ala Val Ile Asp Pro Asn Arg His Thr Val Arg Gly Pro
290 295 300
Val Ala Gly Lys Thr Ala Tyr Gln Ile Ala Gln Met Ala Gly Leu Lys
305 310 315 320
Asp Val Ala Lys Asp Cys Arg Val Leu Ile Ala Glu Ile Asn Gly Val
325 330 335
Gly Ile Lys Tyr Pro Leu Ser Gly Glu Lys Leu Ser Pro Val Leu Thr
340 345 350
Val Tyr Lys Ala Asp Ser His Glu Ala Ala Phe Lys Arg Ala Asn Glu
355 360 365
Leu Leu His Tyr Gly Gly Leu Gly His Thr Ala Gly Ile His Thr Thr
370 375 380
Asp Asp Ala Leu Val Lys Glu Phe Gly Leu Gln Met Pro Ala Cys Arg
385 390 395 400
Ile Leu Val Asn Thr Pro Ser Ser Val Gly Gly Leu Gly Asn Ile Tyr
405 410 415
Asn Asn Met Ala Pro Ser Leu Thr Leu Gly Thr Gly Ser Tyr Gly Gly
420 425 430
Asn Ser Ile Ser His Asn Val Thr Asp Met Asp Leu Ile Asn Ile Lys
435 440 445
Thr Val Ala Lys Arg Arg Asn Asn Met Gln Trp Val Lys Met Pro Pro
450 455 460
Lys Val Tyr Phe Glu Arg Asn Ser Val Arg Tyr Leu Glu His Met Ala
465 470 475 480
Gly Ile Lys Lys Val Phe Leu Val Cys Asp Pro Gly Met Val Glu Phe
485 490 495
Gly Tyr Ala Asp Arg Val Thr Ala Val Leu Asn Lys Arg Thr Asp Pro
500 505 510
Val Asp Ile Asp Ile Phe Ser Glu Val Glu Pro Asn Pro Ser Thr Asp
515 520 525
Thr Val Tyr Lys Gly Val Ala Arg Met Lys Ala Phe Lys Pro Asp Thr
530 535 540
Ile Ile Ala Leu Gly Gly Gly Ser Ala Met Asp Ala Ala Lys Gly Met
545 550 555 560
Trp Leu Phe Tyr Glu His Pro Glu Ala Ser Phe Leu Gly Ala Lys Gln
565 570 575
Lys Phe Leu Asp Ile Arg Lys Arg Thr Tyr Lys Val Pro Val Ser Glu
580 585 590
Lys Val Thr Tyr Ile Gly Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu
595 600 605
Val Thr Pro Tyr Ala Val Ile Thr Asp Ser Lys Thr His Val Lys Tyr
610 615 620
Pro Ile Thr Asp Tyr Ala Met Gln Pro Asp Ile Ala Ile Val Asp Pro
625 630 635 640
Gln Phe Val Glu Thr Val Pro Lys Arg Thr Thr Ala Trp Thr Gly Leu
645 650 655
Asp Val Ile Thr His Ala Thr Glu Ala Tyr Val Ser Thr Met Ala Ser
660 665 670
Asp Phe Thr Arg Gly Trp Ser Ile Gln Ala Leu Gln Leu Ala Phe Lys
675 680 685
Tyr Leu Lys Ala Ser Tyr Asp Gly Asp Lys Met Ala Arg Glu Lys Met
690 695 700
His Asn Ala Ser Thr Leu Ala Gly Met Ala Phe Ala Asn Ala Phe Leu
705 710 715 720
Gly Ile Asn His Ser Ile Ala His Lys Leu Gly Gly Glu Phe Asn Leu
725 730 735
Pro His Gly Leu Ala Ile Ala Ile Thr Tyr Pro Gln Val Val Arg Tyr
740 745 750
Asn Ala Glu Ile Pro Thr Lys Leu Ala Met Trp Pro Lys Tyr Asn His
755 760 765
Asn Thr Ala Leu Ala Asp Tyr Ala Asn Ile Ala Arg Ala Leu Gly Leu
770 775 780
Pro Gly Lys Thr Asp Glu Glu Leu Lys Glu Ser Leu Val Lys Ala Tyr
785 790 795 800
Ile Asp Leu Ala His Ser Met Asp Val Thr Leu Ser Leu Lys Ala Asn
805 810 815
Arg Val Glu Lys Lys His Phe Asp Ala Thr Val Asp Glu Leu Ala Glu
820 825 830
Leu Ala Tyr Glu Asp Gln Cys Thr Thr Ala Asn Pro Arg Glu Pro Leu
835 840 845
Ile Ser Glu Leu Lys Ala Ile Ile Glu Arg Glu Trp Asp Gly Gln Gly
850 855 860
Thr Glu Lys
865
<210> 71
<211> 2703
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(2703)
<223> 肠膜明串珠菌肠膜亚种 ATCC 8293; 基因
LEUM_0146 编码乙醇脱氢酶
<400> 71
atg gca gaa gca att gca aag aaa ccc gca aaa agg gtt ttg acc cct 48
Met Ala Glu Ala Ile Ala Lys Lys Pro Ala Lys Arg Val Leu Thr Pro
1 5 10 15
gaa gaa aaa gcg gaa tta caa aca caa gct gag aag atg act gag gta 96
Glu Glu Lys Ala Glu Leu Gln Thr Gln Ala Glu Lys Met Thr Glu Val
20 25 30
ttg att gaa aaa tca caa aag gca ttg tct gaa ttt tca aca ttt tcg 144
Leu Ile Glu Lys Ser Gln Lys Ala Leu Ser Glu Phe Ser Thr Phe Ser
35 40 45
caa gaa caa gtt gat aaa att gtt gca gct atg gcc ttg gca ggt tct 192
Gln Glu Gln Val Asp Lys Ile Val Ala Ala Met Ala Leu Ala Gly Ser
50 55 60
gag aat tca ctt ctg tta gcc cat gct gct cac gac gag act gga cgt 240
Glu Asn Ser Leu Leu Leu Ala His Ala Ala His Asp Glu Thr Gly Arg
65 70 75 80
ggg gtt gtg gaa gat aag gat acg aaa aat cgt ttc gcc tca gaa tca 288
Gly Val Val Glu Asp Lys Asp Thr Lys Asn Arg Phe Ala Ser Glu Ser
85 90 95
gtt tat aac gct att aag ttt gat aag act gtg ggt gtt att agt gaa 336
Val Tyr Asn Ala Ile Lys Phe Asp Lys Thr Val Gly Val Ile Ser Glu
100 105 110
gac aag att caa ggt aag gta gaa tta gca gcc cca ctt ggt att ttg 384
Asp Lys Ile Gln Gly Lys Val Glu Leu Ala Ala Pro Leu Gly Ile Leu
115 120 125
gct gga atc gtc cca acg aca aat cca acg tcg aca act att ttc aaa 432
Ala Gly Ile Val Pro Thr Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys
130 135 140
tca atg ttg aca gca aag aca cgt aac aca att atc ttt gct ttc cat 480
Ser Met Leu Thr Ala Lys Thr Arg Asn Thr Ile Ile Phe Ala Phe His
145 150 155 160
ccc cag gct caa aaa gca tcg gtt ctt gct gca aaa att gtt tat gat 528
Pro Gln Ala Gln Lys Ala Ser Val Leu Ala Ala Lys Ile Val Tyr Asp
165 170 175
gct gct gtt aaa gca ggc gca ccg gaa aac ttt atc caa tgg att gaa 576
Ala Ala Val Lys Ala Gly Ala Pro Glu Asn Phe Ile Gln Trp Ile Glu
180 185 190
aag cct tca ctt tat gca aca agt gcg ctg ata caa aat cct cac att 624
Lys Pro Ser Leu Tyr Ala Thr Ser Ala Leu Ile Gln Asn Pro His Ile
195 200 205
gct tca att cta gct act ggt ggg cca tca atg gtt aat gca gct ttg 672
Ala Ser Ile Leu Ala Thr Gly Gly Pro Ser Met Val Asn Ala Ala Leu
210 215 220
aag tca gga aat cca tcc atg ggt gtc ggt gct gga aac ggt gca gtt 720
Lys Ser Gly Asn Pro Ser Met Gly Val Gly Ala Gly Asn Gly Ala Val
225 230 235 240
tat att gat gca act gtt gac aca gat cgt gcc gtg tcc gat ttg ttg 768
Tyr Ile Asp Ala Thr Val Asp Thr Asp Arg Ala Val Ser Asp Leu Leu
245 250 255
tta tca aag cgt ttc gat aat ggc atg att tgt gcc aca gaa aac tca 816
Leu Ser Lys Arg Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser
260 265 270
gcc gtt att caa gca cca atc tat gac gaa att tta act aag tta caa 864
Ala Val Ile Gln Ala Pro Ile Tyr Asp Glu Ile Leu Thr Lys Leu Gln
275 280 285
gaa caa ggt gca tac ctt gtt cct aag aaa gac tac aaa aaa att gct 912
Glu Gln Gly Ala Tyr Leu Val Pro Lys Lys Asp Tyr Lys Lys Ile Ala
290 295 300
gat tat gta ttt aag cct aac gca gag gga ttt ggt att gct ggt cct 960
Asp Tyr Val Phe Lys Pro Asn Ala Glu Gly Phe Gly Ile Ala Gly Pro
305 310 315 320
gtt gct ggt atg tca gga cgt tgg att gct gag caa gca ggc gta aag 1008
Val Ala Gly Met Ser Gly Arg Trp Ile Ala Glu Gln Ala Gly Val Lys
325 330 335
att cct gat ggt aaa gat gta ctt ttg ttc gaa tta gat cag aag aac 1056
Ile Pro Asp Gly Lys Asp Val Leu Leu Phe Glu Leu Asp Gln Lys Asn
340 345 350
ata ggt gaa gcg tta tct tct gaa aag tta tcg cca tta ctt tca att 1104
Ile Gly Glu Ala Leu Ser Ser Glu Lys Leu Ser Pro Leu Leu Ser Ile
355 360 365
tat aaa gtt gag aag cgt gaa gaa gct att gag act gtt caa tcc ttg 1152
Tyr Lys Val Glu Lys Arg Glu Glu Ala Ile Glu Thr Val Gln Ser Leu
370 375 380
tta aac tat caa ggc gcg ggg cac aac gca gca att caa att ggt tca 1200
Leu Asn Tyr Gln Gly Ala Gly His Asn Ala Ala Ile Gln Ile Gly Ser
385 390 395 400
caa gat gat cca ttc att aaa gag tat gct gac gct att ggt gca tca 1248
Gln Asp Asp Pro Phe Ile Lys Glu Tyr Ala Asp Ala Ile Gly Ala Ser
405 410 415
cgt att ttg gtt aac caa cct gac tca atc ggt ggt gtt gga gat att 1296
Arg Ile Leu Val Asn Gln Pro Asp Ser Ile Gly Gly Val Gly Asp Ile
420 425 430
tac aca gat gct atg cgt cca tcg ttg aca ctt ggt acc gga tca tgg 1344
Tyr Thr Asp Ala Met Arg Pro Ser Leu Thr Leu Gly Thr Gly Ser Trp
435 440 445
ggg aag aat tca ttg tct cat aac tta tca aca tac gac tta ctt aat 1392
Gly Lys Asn Ser Leu Ser His Asn Leu Ser Thr Tyr Asp Leu Leu Asn
450 455 460
att aag acc gtg gct cgc cgc cgt aat cgt cct caa tgg gtt cgt tta 1440
Ile Lys Thr Val Ala Arg Arg Arg Asn Arg Pro Gln Trp Val Arg Leu
465 470 475 480
cct aag gaa gtt tac tac gaa gcc aat gcc att act tac tta caa gac 1488
Pro Lys Glu Val Tyr Tyr Glu Ala Asn Ala Ile Thr Tyr Leu Gln Asp
485 490 495
ttg cct act ata aac cgt gca ttt att gtc gct gat cct ggt atg gtt 1536
Leu Pro Thr Ile Asn Arg Ala Phe Ile Val Ala Asp Pro Gly Met Val
500 505 510
cag ttc gga ttt gtt ggc aga gta cta ggt caa ctt gag tta cgt caa 1584
Gln Phe Gly Phe Val Gly Arg Val Leu Gly Gln Leu Glu Leu Arg Gln
515 520 525
gaa cag gtt gaa aca aat atc tat ggt tca gtt aag cct gac cca act 1632
Glu Gln Val Glu Thr Asn Ile Tyr Gly Ser Val Lys Pro Asp Pro Thr
530 535 540
ttg tca caa gct gtt gaa att gct cgc caa atg gca gac ttc aaa cca 1680
Leu Ser Gln Ala Val Glu Ile Ala Arg Gln Met Ala Asp Phe Lys Pro
545 550 555 560
gat aca gtt att tta ctt ggc ggt ggt tcg gca ctt gac gct ggt aaa 1728
Asp Thr Val Ile Leu Leu Gly Gly Gly Ser Ala Leu Asp Ala Gly Lys
565 570 575
att ggt cgg ttc ttg tac gaa tac tcg aca cgc cat gaa gga att tta 1776
Ile Gly Arg Phe Leu Tyr Glu Tyr Ser Thr Arg His Glu Gly Ile Leu
580 585 590
gaa gat gac gag gcg att aaa gat cta ttc tta gaa cta caa caa aag 1824
Glu Asp Asp Glu Ala Ile Lys Asp Leu Phe Leu Glu Leu Gln Gln Lys
595 600 605
ttt atg gat att cgt aag cga atc gtt aag ttt tac cac gca cgt ttg 1872
Phe Met Asp Ile Arg Lys Arg Ile Val Lys Phe Tyr His Ala Arg Leu
610 615 620
aca caa atg gtt gcg att cca aca act tca ggt act gga tca gaa gtc 1920
Thr Gln Met Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val
625 630 635 640
aca cca ttt gcc gtt att aca gat gat gaa aca cat gta aag tat cca 1968
Thr Pro Phe Ala Val Ile Thr Asp Asp Glu Thr His Val Lys Tyr Pro
645 650 655
cta gcc gat tat gaa ttg aca ccg gaa gtt gct att gtt gat cca gaa 2016
Leu Ala Asp Tyr Glu Leu Thr Pro Glu Val Ala Ile Val Asp Pro Glu
660 665 670
ttt gtt atg acc gta cca caa cac acg gta tct tgg tca gga tta gat 2064
Phe Val Met Thr Val Pro Gln His Thr Val Ser Trp Ser Gly Leu Asp
675 680 685
gct ttg tca cat gct ttg gaa tcg tat gtc tca gtg atg gct tct gaa 2112
Ala Leu Ser His Ala Leu Glu Ser Tyr Val Ser Val Met Ala Ser Glu
690 695 700
ttc aca cgt cct tgg gca tta caa gct att aag ttg att ttt gat aac 2160
Phe Thr Arg Pro Trp Ala Leu Gln Ala Ile Lys Leu Ile Phe Asp Asn
705 710 715 720
tta aca aat tca tac aat tat gat cct aaa cac cca act aag gaa ggt 2208
Leu Thr Asn Ser Tyr Asn Tyr Asp Pro Lys His Pro Thr Lys Glu Gly
725 730 735
cag aat gca cgc aca aag atg cac tat gcg tca aca ttg gct ggt atg 2256
Gln Asn Ala Arg Thr Lys Met His Tyr Ala Ser Thr Leu Ala Gly Met
740 745 750
tca ttt gcg aat gcc ttc ttg gga ctt aac cac tca cta gca cac aaa 2304
Ser Phe Ala Asn Ala Phe Leu Gly Leu Asn His Ser Leu Ala His Lys
755 760 765
act ggt gga gaa ttc gga cta cct cac ggt atg gca atc gct att gca 2352
Thr Gly Gly Glu Phe Gly Leu Pro His Gly Met Ala Ile Ala Ile Ala
770 775 780
atg cca cat gtg att aag ttt aat gcg gta aca gga aat gta aag cgc 2400
Met Pro His Val Ile Lys Phe Asn Ala Val Thr Gly Asn Val Lys Arg
785 790 795 800
aca cca tac cca cgt tac gaa acc tat aca gca caa aaa gat tat gct 2448
Thr Pro Tyr Pro Arg Tyr Glu Thr Tyr Thr Ala Gln Lys Asp Tyr Ala
805 810 815
gat att gca cgt tac tta ggt ttg aaa ggt gaa aca gat gct gaa ttg 2496
Asp Ile Ala Arg Tyr Leu Gly Leu Lys Gly Glu Thr Asp Ala Glu Leu
820 825 830
gtc gat gta ttg att gca gaa atc aag aag ttg gct gca tca gtg ggt 2544
Val Asp Val Leu Ile Ala Glu Ile Lys Lys Leu Ala Ala Ser Val Gly
835 840 845
gtc aat caa aca cta tct ggc aac ggt gtt tca aag cat gac ttt gat 2592
Val Asn Gln Thr Leu Ser Gly Asn Gly Val Ser Lys His Asp Phe Asp
850 855 860
aca aag tta gaa aag atg att gac tta gtt tac aat gac caa tgc acg 2640
Thr Lys Leu Glu Lys Met Ile Asp Leu Val Tyr Asn Asp Gln Cys Thr
865 870 875 880
ccg gga aac cct cgc caa cca agc ttg gca gaa att cgt caa ttg ttg 2688
Pro Gly Asn Pro Arg Gln Pro Ser Leu Ala Glu Ile Arg Gln Leu Leu
885 890 895
aaa gat cag ttt taa 2703
Lys Asp Gln Phe
900
<210> 72
<211> 900
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 72
Met Ala Glu Ala Ile Ala Lys Lys Pro Ala Lys Arg Val Leu Thr Pro
1 5 10 15
Glu Glu Lys Ala Glu Leu Gln Thr Gln Ala Glu Lys Met Thr Glu Val
20 25 30
Leu Ile Glu Lys Ser Gln Lys Ala Leu Ser Glu Phe Ser Thr Phe Ser
35 40 45
Gln Glu Gln Val Asp Lys Ile Val Ala Ala Met Ala Leu Ala Gly Ser
50 55 60
Glu Asn Ser Leu Leu Leu Ala His Ala Ala His Asp Glu Thr Gly Arg
65 70 75 80
Gly Val Val Glu Asp Lys Asp Thr Lys Asn Arg Phe Ala Ser Glu Ser
85 90 95
Val Tyr Asn Ala Ile Lys Phe Asp Lys Thr Val Gly Val Ile Ser Glu
100 105 110
Asp Lys Ile Gln Gly Lys Val Glu Leu Ala Ala Pro Leu Gly Ile Leu
115 120 125
Ala Gly Ile Val Pro Thr Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys
130 135 140
Ser Met Leu Thr Ala Lys Thr Arg Asn Thr Ile Ile Phe Ala Phe His
145 150 155 160
Pro Gln Ala Gln Lys Ala Ser Val Leu Ala Ala Lys Ile Val Tyr Asp
165 170 175
Ala Ala Val Lys Ala Gly Ala Pro Glu Asn Phe Ile Gln Trp Ile Glu
180 185 190
Lys Pro Ser Leu Tyr Ala Thr Ser Ala Leu Ile Gln Asn Pro His Ile
195 200 205
Ala Ser Ile Leu Ala Thr Gly Gly Pro Ser Met Val Asn Ala Ala Leu
210 215 220
Lys Ser Gly Asn Pro Ser Met Gly Val Gly Ala Gly Asn Gly Ala Val
225 230 235 240
Tyr Ile Asp Ala Thr Val Asp Thr Asp Arg Ala Val Ser Asp Leu Leu
245 250 255
Leu Ser Lys Arg Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser
260 265 270
Ala Val Ile Gln Ala Pro Ile Tyr Asp Glu Ile Leu Thr Lys Leu Gln
275 280 285
Glu Gln Gly Ala Tyr Leu Val Pro Lys Lys Asp Tyr Lys Lys Ile Ala
290 295 300
Asp Tyr Val Phe Lys Pro Asn Ala Glu Gly Phe Gly Ile Ala Gly Pro
305 310 315 320
Val Ala Gly Met Ser Gly Arg Trp Ile Ala Glu Gln Ala Gly Val Lys
325 330 335
Ile Pro Asp Gly Lys Asp Val Leu Leu Phe Glu Leu Asp Gln Lys Asn
340 345 350
Ile Gly Glu Ala Leu Ser Ser Glu Lys Leu Ser Pro Leu Leu Ser Ile
355 360 365
Tyr Lys Val Glu Lys Arg Glu Glu Ala Ile Glu Thr Val Gln Ser Leu
370 375 380
Leu Asn Tyr Gln Gly Ala Gly His Asn Ala Ala Ile Gln Ile Gly Ser
385 390 395 400
Gln Asp Asp Pro Phe Ile Lys Glu Tyr Ala Asp Ala Ile Gly Ala Ser
405 410 415
Arg Ile Leu Val Asn Gln Pro Asp Ser Ile Gly Gly Val Gly Asp Ile
420 425 430
Tyr Thr Asp Ala Met Arg Pro Ser Leu Thr Leu Gly Thr Gly Ser Trp
435 440 445
Gly Lys Asn Ser Leu Ser His Asn Leu Ser Thr Tyr Asp Leu Leu Asn
450 455 460
Ile Lys Thr Val Ala Arg Arg Arg Asn Arg Pro Gln Trp Val Arg Leu
465 470 475 480
Pro Lys Glu Val Tyr Tyr Glu Ala Asn Ala Ile Thr Tyr Leu Gln Asp
485 490 495
Leu Pro Thr Ile Asn Arg Ala Phe Ile Val Ala Asp Pro Gly Met Val
500 505 510
Gln Phe Gly Phe Val Gly Arg Val Leu Gly Gln Leu Glu Leu Arg Gln
515 520 525
Glu Gln Val Glu Thr Asn Ile Tyr Gly Ser Val Lys Pro Asp Pro Thr
530 535 540
Leu Ser Gln Ala Val Glu Ile Ala Arg Gln Met Ala Asp Phe Lys Pro
545 550 555 560
Asp Thr Val Ile Leu Leu Gly Gly Gly Ser Ala Leu Asp Ala Gly Lys
565 570 575
Ile Gly Arg Phe Leu Tyr Glu Tyr Ser Thr Arg His Glu Gly Ile Leu
580 585 590
Glu Asp Asp Glu Ala Ile Lys Asp Leu Phe Leu Glu Leu Gln Gln Lys
595 600 605
Phe Met Asp Ile Arg Lys Arg Ile Val Lys Phe Tyr His Ala Arg Leu
610 615 620
Thr Gln Met Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val
625 630 635 640
Thr Pro Phe Ala Val Ile Thr Asp Asp Glu Thr His Val Lys Tyr Pro
645 650 655
Leu Ala Asp Tyr Glu Leu Thr Pro Glu Val Ala Ile Val Asp Pro Glu
660 665 670
Phe Val Met Thr Val Pro Gln His Thr Val Ser Trp Ser Gly Leu Asp
675 680 685
Ala Leu Ser His Ala Leu Glu Ser Tyr Val Ser Val Met Ala Ser Glu
690 695 700
Phe Thr Arg Pro Trp Ala Leu Gln Ala Ile Lys Leu Ile Phe Asp Asn
705 710 715 720
Leu Thr Asn Ser Tyr Asn Tyr Asp Pro Lys His Pro Thr Lys Glu Gly
725 730 735
Gln Asn Ala Arg Thr Lys Met His Tyr Ala Ser Thr Leu Ala Gly Met
740 745 750
Ser Phe Ala Asn Ala Phe Leu Gly Leu Asn His Ser Leu Ala His Lys
755 760 765
Thr Gly Gly Glu Phe Gly Leu Pro His Gly Met Ala Ile Ala Ile Ala
770 775 780
Met Pro His Val Ile Lys Phe Asn Ala Val Thr Gly Asn Val Lys Arg
785 790 795 800
Thr Pro Tyr Pro Arg Tyr Glu Thr Tyr Thr Ala Gln Lys Asp Tyr Ala
805 810 815
Asp Ile Ala Arg Tyr Leu Gly Leu Lys Gly Glu Thr Asp Ala Glu Leu
820 825 830
Val Asp Val Leu Ile Ala Glu Ile Lys Lys Leu Ala Ala Ser Val Gly
835 840 845
Val Asn Gln Thr Leu Ser Gly Asn Gly Val Ser Lys His Asp Phe Asp
850 855 860
Thr Lys Leu Glu Lys Met Ile Asp Leu Val Tyr Asn Asp Gln Cys Thr
865 870 875 880
Pro Gly Asn Pro Arg Gln Pro Ser Leu Ala Glu Ile Arg Gln Leu Leu
885 890 895
Lys Asp Gln Phe
900
<210> 73
<211> 927
<212> DNA
<213> 嗜热链球菌（Streptococcus thermophilus）
<220>
<221> CDS
<222> (1)..(927)
<223> 嗜热链球菌 CNRZ1066; 基因 str0936 编码乙醇脱氢酶
<220>
<221> tRNA
<222> (1)..(3)
<223> 由起始密码子（GTG）处的tRNA插入Met而不是Val
<400> 73
gtg gat ctg acc ttt gga gtt acc gta atc cgg aaa tgg aag agg gac 48
Val Asp Leu Thr Phe Gly Val Thr Val Ile Arg Lys Trp Lys Arg Asp
1 5 10 15
ata gaa aat agt ggt cat gag gct atc gga att gtc gaa gaa gtt ggt 96
Ile Glu Asn Ser Gly His Glu Ala Ile Gly Ile Val Glu Glu Val Gly
20 25 30
gag caa gtt act acg gta aaa cca gga gac ttt gtc att gca cct ttc 144
Glu Gln Val Thr Thr Val Lys Pro Gly Asp Phe Val Ile Ala Pro Phe
35 40 45
aca cat ggg tgt ggt cag tgt gat gct tgt cga gct ggt ttt gac ggc 192
Thr His Gly Cys Gly Gln Cys Asp Ala Cys Arg Ala Gly Phe Asp Gly
50 55 60
tct tgt gat aac cac tca ggt aat aac tgg tct gag ggc gtg cag gct 240
Ser Cys Asp Asn His Ser Gly Asn Asn Trp Ser Glu Gly Val Gln Ala
65 70 75 80
gag tat atc cgt ttc cac ttt gcc aac tgg gcc ctt gtc aaa att ctt 288
Glu Tyr Ile Arg Phe His Phe Ala Asn Trp Ala Leu Val Lys Ile Leu
85 90 95
ggg caa ccc tca gat tat agc gaa ggc atg ctc aag tca ctt ttg aca 336
Gly Gln Pro Ser Asp Tyr Ser Glu Gly Met Leu Lys Ser Leu Leu Thr
100 105 110
ctt gct gat gtt atg ccg acg ggt tac cat gct gcg cgt gtg gct aat 384
Leu Ala Asp Val Met Pro Thr Gly Tyr His Ala Ala Arg Val Ala Asn
115 120 125
gta caa aag ggt gat aag gtt gtc gtt atc gga gat ggg gct gtt ggt 432
Val Gln Lys Gly Asp Lys Val Val Val Ile Gly Asp Gly Ala Val Gly
130 135 140
caa tgt gct gtc att gca tct aag atg cgt gga gct tct caa att gtc 480
Gln Cys Ala Val Ile Ala Ser Lys Met Arg Gly Ala Ser Gln Ile Val
145 150 155 160
ctt atg agc cgt cac gaa gat cgt caa aag atg gct ttg gag tca ggt 528
Leu Met Ser Arg His Glu Asp Arg Gln Lys Met Ala Leu Glu Ser Gly
165 170 175
gcg act gcg gtt gta gct gag cgt ggc gaa aaa ggc att gac aaa gtc 576
Ala Thr Ala Val Val Ala Glu Arg Gly Glu Lys Gly Ile Asp Lys Val
180 185 190
cgt gaa gtc ctc ggg gga gga gca gat gca gct ctc gaa tgt gtc ggt 624
Arg Glu Val Leu Gly Gly Gly Ala Asp Ala Ala Leu Glu Cys Val Gly
195 200 205
act gaa gca gca gta gaa caa gct ctt ggt gtc ctc cat aac ggt ggt 672
Thr Glu Ala Ala Val Glu Gln Ala Leu Gly Val Leu His Asn Gly Gly
210 215 220
cgt atg gga ttt gtc ggt gtg cct cat tat aat aac cgt tcc ctt ggt 720
Arg Met Gly Phe Val Gly Val Pro His Tyr Asn Asn Arg Ser Leu Gly
225 230 235 240
tcg acc ttt gcc caa aat att tta gtg gcg gga ggg gta gcc tct gtc 768
Ser Thr Phe Ala Gln Asn Ile Leu Val Ala Gly Gly Val Ala Ser Val
245 250 255
aca acc tat gat aaa caa gtt ttg tta aaa gct gtt ctt gat ggc gac 816
Thr Thr Tyr Asp Lys Gln Val Leu Leu Lys Ala Val Leu Asp Gly Asp
260 265 270
atc aat cca ggc cga gtc ttt acg aca agt tat aaa ttg gaa gat att 864
Ile Asn Pro Gly Arg Val Phe Thr Thr Ser Tyr Lys Leu Glu Asp Ile
275 280 285
aat cag gct tat aaa gat atg gat gaa cgc aag tct att aaa tct atg 912
Asn Gln Ala Tyr Lys Asp Met Asp Glu Arg Lys Ser Ile Lys Ser Met
290 295 300
att gtt tta gat tag 927
Ile Val Leu Asp
305
<210> 74
<211> 308
<212> PRT
<213> 嗜热链球菌（Streptococcus thermophilus）
<400> 74
Val Asp Leu Thr Phe Gly Val Thr Val Ile Arg Lys Trp Lys Arg Asp
1 5 10 15
Ile Glu Asn Ser Gly His Glu Ala Ile Gly Ile Val Glu Glu Val Gly
20 25 30
Glu Gln Val Thr Thr Val Lys Pro Gly Asp Phe Val Ile Ala Pro Phe
35 40 45
Thr His Gly Cys Gly Gln Cys Asp Ala Cys Arg Ala Gly Phe Asp Gly
50 55 60
Ser Cys Asp Asn His Ser Gly Asn Asn Trp Ser Glu Gly Val Gln Ala
65 70 75 80
Glu Tyr Ile Arg Phe His Phe Ala Asn Trp Ala Leu Val Lys Ile Leu
85 90 95
Gly Gln Pro Ser Asp Tyr Ser Glu Gly Met Leu Lys Ser Leu Leu Thr
100 105 110
Leu Ala Asp Val Met Pro Thr Gly Tyr His Ala Ala Arg Val Ala Asn
115 120 125
Val Gln Lys Gly Asp Lys Val Val Val Ile Gly Asp Gly Ala Val Gly
130 135 140
Gln Cys Ala Val Ile Ala Ser Lys Met Arg Gly Ala Ser Gln Ile Val
145 150 155 160
Leu Met Ser Arg His Glu Asp Arg Gln Lys Met Ala Leu Glu Ser Gly
165 170 175
Ala Thr Ala Val Val Ala Glu Arg Gly Glu Lys Gly Ile Asp Lys Val
180 185 190
Arg Glu Val Leu Gly Gly Gly Ala Asp Ala Ala Leu Glu Cys Val Gly
195 200 205
Thr Glu Ala Ala Val Glu Gln Ala Leu Gly Val Leu His Asn Gly Gly
210 215 220
Arg Met Gly Phe Val Gly Val Pro His Tyr Asn Asn Arg Ser Leu Gly
225 230 235 240
Ser Thr Phe Ala Gln Asn Ile Leu Val Ala Gly Gly Val Ala Ser Val
245 250 255
Thr Thr Tyr Asp Lys Gln Val Leu Leu Lys Ala Val Leu Asp Gly Asp
260 265 270
Ile Asn Pro Gly Arg Val Phe Thr Thr Ser Tyr Lys Leu Glu Asp Ile
275 280 285
Asn Gln Ala Tyr Lys Asp Met Asp Glu Arg Lys Ser Ile Lys Ser Met
290 295 300
Ile Val Leu Asp
305
<210> 75
<211> 2697
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(2697)
<223> 酒类酒球菌 PSU-1; 基因 OEOE_1248 编码乙醇脱氢酶
<400> 75
atg gca gtt gaa acg aag aag att act aaa aag ata ctg act tcc gac 48
Met Ala Val Glu Thr Lys Lys Ile Thr Lys Lys Ile Leu Thr Ser Asp
1 5 10 15
gaa ctt aaa gac aaa aag gag aca gca aaa aaa tac att gga gaa tta 96
Glu Leu Lys Asp Lys Lys Glu Thr Ala Lys Lys Tyr Ile Gly Glu Leu
20 25 30
gtc gat aaa tca aga cag gca tta tta gaa ttc tct caa tat agt caa 144
Val Asp Lys Ser Arg Gln Ala Leu Leu Glu Phe Ser Gln Tyr Ser Gln
35 40 45
tca caa gtt gac aaa att gtt gcc gca atg gct tta gcc ggt tcc gaa 192
Ser Gln Val Asp Lys Ile Val Ala Ala Met Ala Leu Ala Gly Ser Glu
50 55 60
cat tct ttg gaa tta gca cac ttt gct cga aat gaa act gga aga gga 240
His Ser Leu Glu Leu Ala His Phe Ala Arg Asn Glu Thr Gly Arg Gly
65 70 75 80
gtt gtt gaa gac aaa gat acg aaa aat cgt ttt gcc tcg gaa tct gta 288
Val Val Glu Asp Lys Asp Thr Lys Asn Arg Phe Ala Ser Glu Ser Val
85 90 95
tat aac acg att aaa aat gac aaa aca gtt ggc gta att gat gaa gac 336
Tyr Asn Thr Ile Lys Asn Asp Lys Thr Val Gly Val Ile Asp Glu Asp
100 105 110
cca atc act ggc aaa gtt cag tta gct gca cca ctt ggg att tta gcc 384
Pro Ile Thr Gly Lys Val Gln Leu Ala Ala Pro Leu Gly Ile Leu Ala
115 120 125
gga att gtt cca acg aca aat cca acg tct acc acg att ttt aaa tca 432
Gly Ile Val Pro Thr Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser
130 135 140
ttg tta acc gct aag aca cgt aat acg atc att ttt gct ttt cac ccg 480
Leu Leu Thr Ala Lys Thr Arg Asn Thr Ile Ile Phe Ala Phe His Pro
145 150 155 160
caa gca caa aaa tcg tct gta gcg gct gcg aaa att gtt tac cag gcg 528
Gln Ala Gln Lys Ser Ser Val Ala Ala Ala Lys Ile Val Tyr Gln Ala
165 170 175
gct aga aaa gct gga gca ccg aaa gat ttc att cag tgg atc gaa acg 576
Ala Arg Lys Ala Gly Ala Pro Lys Asp Phe Ile Gln Trp Ile Glu Thr
180 185 190
cct tcc ttg gaa aat acg act gct ttg atg caa aat cct gaa ata gct 624
Pro Ser Leu Glu Asn Thr Thr Ala Leu Met Gln Asn Pro Glu Ile Ala
195 200 205
tca att ttg gct act gga ggc cct tct atg gtt cat gcc gca tta aca 672
Ser Ile Leu Ala Thr Gly Gly Pro Ser Met Val His Ala Ala Leu Thr
210 215 220
tcg gga aac cct tca atg ggt gtt ggg gca gga aat gga gct gtt ttc 720
Ser Gly Asn Pro Ser Met Gly Val Gly Ala Gly Asn Gly Ala Val Phe
225 230 235 240
att gac cat acg gct cgt gtt cgt cga gcg gca gaa gac ttg ttg cta 768
Ile Asp His Thr Ala Arg Val Arg Arg Ala Ala Glu Asp Leu Leu Leu
245 250 255
tca aaa cgt ttt gat aat gga atg att tgc gct aca gag aat tcc gca 816
Ser Lys Arg Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser Ala
260 265 270
gtt atc gaa gct tcg gtt tat gac gaa ttt atg aaa tta atg cag gaa 864
Val Ile Glu Ala Ser Val Tyr Asp Glu Phe Met Lys Leu Met Gln Glu
275 280 285
aag ggt act tat ctc gtt ccg aag gat gat tac aaa aag atc gct gat 912
Lys Gly Thr Tyr Leu Val Pro Lys Asp Asp Tyr Lys Lys Ile Ala Asp
290 295 300
ttt gtt ttc agc gat aaa cat gca gtt aat ggt ccg gtt gcc ggg atg 960
Phe Val Phe Ser Asp Lys His Ala Val Asn Gly Pro Val Ala Gly Met
305 310 315 320
agt ggc cgt tgg att gct gaa cat gcc ggt gtt act ttg ccg aag ggc 1008
Ser Gly Arg Trp Ile Ala Glu His Ala Gly Val Thr Leu Pro Lys Gly
325 330 335
aaa gat gtt ttg ttg ttt gag ttg gat caa gac gac att ggc gaa aaa 1056
Lys Asp Val Leu Leu Phe Glu Leu Asp Gln Asp Asp Ile Gly Glu Lys
340 345 350
ctt tcc tcg gaa aaa ctt agc ccc ttg ctt tcg gtc tat aaa gct gct 1104
Leu Ser Ser Glu Lys Leu Ser Pro Leu Leu Ser Val Tyr Lys Ala Ala
355 360 365
gat cgt aaa gaa gcg ata aaa gtt gtt caa aga ctt ttg aac tat caa 1152
Asp Arg Lys Glu Ala Ile Lys Val Val Gln Arg Leu Leu Asn Tyr Gln
370 375 380
gga gct ggt cat aat gcg gca atc cag att ggc gct caa gac gat ccc 1200
Gly Ala Gly His Asn Ala Ala Ile Gln Ile Gly Ala Gln Asp Asp Pro
385 390 395 400
ttt gtt aaa gaa tat gcc gat gca gtt tct gca tct aga att ttg gtt 1248
Phe Val Lys Glu Tyr Ala Asp Ala Val Ser Ala Ser Arg Ile Leu Val
405 410 415
aac cag ccc gat tca ata ggt gga gtc ggc gat att tat aca gat gca 1296
Asn Gln Pro Asp Ser Ile Gly Gly Val Gly Asp Ile Tyr Thr Asp Ala
420 425 430
ttg cgt cca agt atg acg ctt ggt act ggt tca tgg ggg aag aat tca 1344
Leu Arg Pro Ser Met Thr Leu Gly Thr Gly Ser Trp Gly Lys Asn Ser
435 440 445
ttg tca cat aat tta tca act tac gat ttg ttg aat gtc aaa acg gtt 1392
Leu Ser His Asn Leu Ser Thr Tyr Asp Leu Leu Asn Val Lys Thr Val
450 455 460
gcc cgc aga cgt aat cgt cct caa tgg gtt cgt ctt cca aaa gat atc 1440
Ala Arg Arg Arg Asn Arg Pro Gln Trp Val Arg Leu Pro Lys Asp Ile
465 470 475 480
tac tat gag aaa aat gcc att acc tat ctg cag gaa tta ccg aac gtc 1488
Tyr Tyr Glu Lys Asn Ala Ile Thr Tyr Leu Gln Glu Leu Pro Asn Val
485 490 495
aat cgg gct ttc gtt att acc gat cat tcg ttg gtc aaa tat gga ttc 1536
Asn Arg Ala Phe Val Ile Thr Asp His Ser Leu Val Lys Tyr Gly Phe
500 505 510
gtc gat cgg att ctt gag caa ttc gaa ttg cgt ccc gac cag gtt aaa 1584
Val Asp Arg Ile Leu Glu Gln Phe Glu Leu Arg Pro Asp Gln Val Lys
515 520 525
aca agt att tac agt tct gtt caa ccg gat cct tat ttg agt cag gct 1632
Thr Ser Ile Tyr Ser Ser Val Gln Pro Asp Pro Tyr Leu Ser Gln Ala
530 535 540
gtt gaa att gca aaa caa atg cag gag ttc gaa ccg gat aca gta att 1680
Val Glu Ile Ala Lys Gln Met Gln Glu Phe Glu Pro Asp Thr Val Ile
545 550 555 560
gct ctt gga gga ggt tcc tct ttg gat gtt gcc aag att tcc cgt ttt 1728
Ala Leu Gly Gly Gly Ser Ser Leu Asp Val Ala Lys Ile Ser Arg Phe
565 570 575
ctt tat gaa tat tct caa gaa ccg gac cac att ggt ttt ttg gaa aac 1776
Leu Tyr Glu Tyr Ser Gln Glu Pro Asp His Ile Gly Phe Leu Glu Asn
580 585 590
att gac gat att aaa gaa ttg ttt aaa gga ttg cag caa aag ttt atg 1824
Ile Asp Asp Ile Lys Glu Leu Phe Lys Gly Leu Gln Gln Lys Phe Met
595 600 605
gat att cgg aaa cgg att gtt aaa ttc gaa cat cag aat ttg act caa 1872
Asp Ile Arg Lys Arg Ile Val Lys Phe Glu His Gln Asn Leu Thr Gln
610 615 620
ctg gtt gct att cca aca act tcg ggt acc ggt tcg gaa gta aca ccg 1920
Leu Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro
625 630 635 640
ttt tcg gtt atc acc gat gat gaa act cac gtt aaa tac cct ttg gcc 1968
Phe Ser Val Ile Thr Asp Asp Glu Thr His Val Lys Tyr Pro Leu Ala
645 650 655
gat tat gaa tta act ccg caa gtt gca atc gtt gat cct gaa ttg gtt 2016
Asp Tyr Glu Leu Thr Pro Gln Val Ala Ile Val Asp Pro Glu Leu Val
660 665 670
atg act gta cca aaa cgg acc ctg gct tgg tct gga ctg gac gcc tta 2064
Met Thr Val Pro Lys Arg Thr Leu Ala Trp Ser Gly Leu Asp Ala Leu
675 680 685
tct cac tcg ctt gaa tcc tac gtg tcg gtt atg agt tct gaa ttt aca 2112
Ser His Ser Leu Glu Ser Tyr Val Ser Val Met Ser Ser Glu Phe Thr
690 695 700
cgg ccg tgg gct tta cag gca atc aaa ctg att ttt gaa aac atc gtt 2160
Arg Pro Trp Ala Leu Gln Ala Ile Lys Leu Ile Phe Glu Asn Ile Val
705 710 715 720
gat tcc tat aat tac gat cca aag cac cca act aat cgg ggc gcc gag 2208
Asp Ser Tyr Asn Tyr Asp Pro Lys His Pro Thr Asn Arg Gly Ala Glu
725 730 735
gca cgt gaa aaa atg cat tat gct gca act ttg gcc ggc atg tct ttc 2256
Ala Arg Glu Lys Met His Tyr Ala Ala Thr Leu Ala Gly Met Ser Phe
740 745 750
gga aac gcc ttc ttg ggt ata aac cat tca ctg gcc cat aaa acc ggt 2304
Gly Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His Lys Thr Gly
755 760 765
gga gag ttc ggt ctt cct cat ggt ttg gca atc agc atc gcg atg cca 2352
Gly Glu Phe Gly Leu Pro His Gly Leu Ala Ile Ser Ile Ala Met Pro
770 775 780
cat gta att cga ttt aat gcg gtt acc gga aac gtc aaa cgg act cct 2400
His Val Ile Arg Phe Asn Ala Val Thr Gly Asn Val Lys Arg Thr Pro
785 790 795 800
ttt cct cgt tat gag gtt tat aca gcc caa aaa gat tat gcc gat att 2448
Phe Pro Arg Tyr Glu Val Tyr Thr Ala Gln Lys Asp Tyr Ala Asp Ile
805 810 815
gcg cgc cat att ggc ctc agc ggt aag aac gat gcc gaa ttg gtt gaa 2496
Ala Arg His Ile Gly Leu Ser Gly Lys Asn Asp Ala Glu Leu Val Glu
820 825 830
aaa ctt atc gcc aaa atc aag gaa tta acc gat gcc ttg gat gtc aat 2544
Lys Leu Ile Ala Lys Ile Lys Glu Leu Thr Asp Ala Leu Asp Val Asn
835 840 845
att act ttg agc ggc aat gga gtc gat aaa aag agc ttt gaa cat tct 2592
Ile Thr Leu Ser Gly Asn Gly Val Asp Lys Lys Ser Phe Glu His Ser
850 855 860
ttg gat caa ctg gtt gat ctt gtt tac gat gac cag tgc acg cca gga 2640
Leu Asp Gln Leu Val Asp Leu Val Tyr Asp Asp Gln Cys Thr Pro Gly
865 870 875 880
aat ccg cga caa ccg aat tta gct gaa att agg cag tta ttg atc gac 2688
Asn Pro Arg Gln Pro Asn Leu Ala Glu Ile Arg Gln Leu Leu Ile Asp
885 890 895
caa ttt taa 2697
Gln Phe
<210> 76
<211> 898
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 76
Met Ala Val Glu Thr Lys Lys Ile Thr Lys Lys Ile Leu Thr Ser Asp
1 5 10 15
Glu Leu Lys Asp Lys Lys Glu Thr Ala Lys Lys Tyr Ile Gly Glu Leu
20 25 30
Val Asp Lys Ser Arg Gln Ala Leu Leu Glu Phe Ser Gln Tyr Ser Gln
35 40 45
Ser Gln Val Asp Lys Ile Val Ala Ala Met Ala Leu Ala Gly Ser Glu
50 55 60
His Ser Leu Glu Leu Ala His Phe Ala Arg Asn Glu Thr Gly Arg Gly
65 70 75 80
Val Val Glu Asp Lys Asp Thr Lys Asn Arg Phe Ala Ser Glu Ser Val
85 90 95
Tyr Asn Thr Ile Lys Asn Asp Lys Thr Val Gly Val Ile Asp Glu Asp
100 105 110
Pro Ile Thr Gly Lys Val Gln Leu Ala Ala Pro Leu Gly Ile Leu Ala
115 120 125
Gly Ile Val Pro Thr Thr Asn Pro Thr Ser Thr Thr Ile Phe Lys Ser
130 135 140
Leu Leu Thr Ala Lys Thr Arg Asn Thr Ile Ile Phe Ala Phe His Pro
145 150 155 160
Gln Ala Gln Lys Ser Ser Val Ala Ala Ala Lys Ile Val Tyr Gln Ala
165 170 175
Ala Arg Lys Ala Gly Ala Pro Lys Asp Phe Ile Gln Trp Ile Glu Thr
180 185 190
Pro Ser Leu Glu Asn Thr Thr Ala Leu Met Gln Asn Pro Glu Ile Ala
195 200 205
Ser Ile Leu Ala Thr Gly Gly Pro Ser Met Val His Ala Ala Leu Thr
210 215 220
Ser Gly Asn Pro Ser Met Gly Val Gly Ala Gly Asn Gly Ala Val Phe
225 230 235 240
Ile Asp His Thr Ala Arg Val Arg Arg Ala Ala Glu Asp Leu Leu Leu
245 250 255
Ser Lys Arg Phe Asp Asn Gly Met Ile Cys Ala Thr Glu Asn Ser Ala
260 265 270
Val Ile Glu Ala Ser Val Tyr Asp Glu Phe Met Lys Leu Met Gln Glu
275 280 285
Lys Gly Thr Tyr Leu Val Pro Lys Asp Asp Tyr Lys Lys Ile Ala Asp
290 295 300
Phe Val Phe Ser Asp Lys His Ala Val Asn Gly Pro Val Ala Gly Met
305 310 315 320
Ser Gly Arg Trp Ile Ala Glu His Ala Gly Val Thr Leu Pro Lys Gly
325 330 335
Lys Asp Val Leu Leu Phe Glu Leu Asp Gln Asp Asp Ile Gly Glu Lys
340 345 350
Leu Ser Ser Glu Lys Leu Ser Pro Leu Leu Ser Val Tyr Lys Ala Ala
355 360 365
Asp Arg Lys Glu Ala Ile Lys Val Val Gln Arg Leu Leu Asn Tyr Gln
370 375 380
Gly Ala Gly His Asn Ala Ala Ile Gln Ile Gly Ala Gln Asp Asp Pro
385 390 395 400
Phe Val Lys Glu Tyr Ala Asp Ala Val Ser Ala Ser Arg Ile Leu Val
405 410 415
Asn Gln Pro Asp Ser Ile Gly Gly Val Gly Asp Ile Tyr Thr Asp Ala
420 425 430
Leu Arg Pro Ser Met Thr Leu Gly Thr Gly Ser Trp Gly Lys Asn Ser
435 440 445
Leu Ser His Asn Leu Ser Thr Tyr Asp Leu Leu Asn Val Lys Thr Val
450 455 460
Ala Arg Arg Arg Asn Arg Pro Gln Trp Val Arg Leu Pro Lys Asp Ile
465 470 475 480
Tyr Tyr Glu Lys Asn Ala Ile Thr Tyr Leu Gln Glu Leu Pro Asn Val
485 490 495
Asn Arg Ala Phe Val Ile Thr Asp His Ser Leu Val Lys Tyr Gly Phe
500 505 510
Val Asp Arg Ile Leu Glu Gln Phe Glu Leu Arg Pro Asp Gln Val Lys
515 520 525
Thr Ser Ile Tyr Ser Ser Val Gln Pro Asp Pro Tyr Leu Ser Gln Ala
530 535 540
Val Glu Ile Ala Lys Gln Met Gln Glu Phe Glu Pro Asp Thr Val Ile
545 550 555 560
Ala Leu Gly Gly Gly Ser Ser Leu Asp Val Ala Lys Ile Ser Arg Phe
565 570 575
Leu Tyr Glu Tyr Ser Gln Glu Pro Asp His Ile Gly Phe Leu Glu Asn
580 585 590
Ile Asp Asp Ile Lys Glu Leu Phe Lys Gly Leu Gln Gln Lys Phe Met
595 600 605
Asp Ile Arg Lys Arg Ile Val Lys Phe Glu His Gln Asn Leu Thr Gln
610 615 620
Leu Val Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Pro
625 630 635 640
Phe Ser Val Ile Thr Asp Asp Glu Thr His Val Lys Tyr Pro Leu Ala
645 650 655
Asp Tyr Glu Leu Thr Pro Gln Val Ala Ile Val Asp Pro Glu Leu Val
660 665 670
Met Thr Val Pro Lys Arg Thr Leu Ala Trp Ser Gly Leu Asp Ala Leu
675 680 685
Ser His Ser Leu Glu Ser Tyr Val Ser Val Met Ser Ser Glu Phe Thr
690 695 700
Arg Pro Trp Ala Leu Gln Ala Ile Lys Leu Ile Phe Glu Asn Ile Val
705 710 715 720
Asp Ser Tyr Asn Tyr Asp Pro Lys His Pro Thr Asn Arg Gly Ala Glu
725 730 735
Ala Arg Glu Lys Met His Tyr Ala Ala Thr Leu Ala Gly Met Ser Phe
740 745 750
Gly Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His Lys Thr Gly
755 760 765
Gly Glu Phe Gly Leu Pro His Gly Leu Ala Ile Ser Ile Ala Met Pro
770 775 780
His Val Ile Arg Phe Asn Ala Val Thr Gly Asn Val Lys Arg Thr Pro
785 790 795 800
Phe Pro Arg Tyr Glu Val Tyr Thr Ala Gln Lys Asp Tyr Ala Asp Ile
805 810 815
Ala Arg His Ile Gly Leu Ser Gly Lys Asn Asp Ala Glu Leu Val Glu
820 825 830
Lys Leu Ile Ala Lys Ile Lys Glu Leu Thr Asp Ala Leu Asp Val Asn
835 840 845
Ile Thr Leu Ser Gly Asn Gly Val Asp Lys Lys Ser Phe Glu His Ser
850 855 860
Leu Asp Gln Leu Val Asp Leu Val Tyr Asp Asp Gln Cys Thr Pro Gly
865 870 875 880
Asn Pro Arg Gln Pro Asn Leu Ala Glu Ile Arg Gln Leu Leu Ile Asp
885 890 895
Gln Phe
<210> 77
<211> 2613
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(2613)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_0619 编码乙醇脱氢酶
<400> 77
atg gca atc gat gaa aaa gtt gtt ggc aaa tct gcg caa atc acg gaa 48
Met Ala Ile Asp Glu Lys Val Val Gly Lys Ser Ala Gln Ile Thr Glu
1 5 10 15
atg gtc gat tcc ctc gtc gca aaa ggg caa aaa gca ctg cgt gaa ttt 96
Met Val Asp Ser Leu Val Ala Lys Gly Gln Lys Ala Leu Arg Glu Phe
20 25 30
atg gaa ctg gat cag gca caa gtg gac aat atc gta aaa caa atg gca 144
Met Glu Leu Asp Gln Ala Gln Val Asp Asn Ile Val Lys Gln Met Ala
35 40 45
ctt gcc ggg ttg gaa cag cat atg gtg ctc gcc aaa atg gcc gtg gaa 192
Leu Ala Gly Leu Glu Gln His Met Val Leu Ala Lys Met Ala Val Glu
50 55 60
gaa aca ggg cgc ggc gta tat gaa gac aaa atg aca aaa aac ttg ttt 240
Glu Thr Gly Arg Gly Val Tyr Glu Asp Lys Met Thr Lys Asn Leu Phe
65 70 75 80
gca aca gaa tac atc tat cac aac att aaa tat aac aaa aca gtc ggc 288
Ala Thr Glu Tyr Ile Tyr His Asn Ile Lys Tyr Asn Lys Thr Val Gly
85 90 95
atc att gac gaa aac aat aat gaa ggg att gtt aaa ttt gcg gaa ccg 336
Ile Ile Asp Glu Asn Asn Asn Glu Gly Ile Val Lys Phe Ala Glu Pro
100 105 110
gtc ggc gtg att gcc ggg gtc act cct gta aca aac ccg aca tca acc 384
Val Gly Val Ile Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr
115 120 125
acg atg ttt aaa gca tta att gcc att aaa aca cgc aac cca att att 432
Thr Met Phe Lys Ala Leu Ile Ala Ile Lys Thr Arg Asn Pro Ile Ile
130 135 140
ttc gcg ttc cat ccg tcc gca caa aaa tgc agc agc cat gcc gca aaa 480
Phe Ala Phe His Pro Ser Ala Gln Lys Cys Ser Ser His Ala Ala Lys
145 150 155 160
gtg atg ctt gac gct gct gtg aaa gcc ggc gcc ccg gaa aac tgc atc 528
Val Met Leu Asp Ala Ala Val Lys Ala Gly Ala Pro Glu Asn Cys Ile
165 170 175
caa tgg atc gaa aaa ccg gcc att gaa gcg aca cag caa ttg atg aac 576
Gln Trp Ile Glu Lys Pro Ala Ile Glu Ala Thr Gln Gln Leu Met Asn
180 185 190
cat tcg ggt att tca ctg att ctt gca acg ggc ggt tcc ggc atg gta 624
His Ser Gly Ile Ser Leu Ile Leu Ala Thr Gly Gly Ser Gly Met Val
195 200 205
aag tcc gca tat tcg agc ggg aaa cct gcg ctt ggc gtc ggg ccg gga 672
Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly
210 215 220
aac gtc cct tgc tat att gaa aaa tct gct gat atc aaa cgc gcc gtc 720
Asn Val Pro Cys Tyr Ile Glu Lys Ser Ala Asp Ile Lys Arg Ala Val
225 230 235 240
aat gat ttg att ttg tcc aaa acg ttt gac aac ggc atg atc tgt gcg 768
Asn Asp Leu Ile Leu Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala
245 250 255
tcg gaa cag gct gtc atc atc gac aaa gaa att tat gac aat gtg aaa 816
Ser Glu Gln Ala Val Ile Ile Asp Lys Glu Ile Tyr Asp Asn Val Lys
260 265 270
aac gaa ttg att gca aat caa tgc tac ttc ctg aat gaa gcg gag aag 864
Asn Glu Leu Ile Ala Asn Gln Cys Tyr Phe Leu Asn Glu Ala Glu Lys
275 280 285
aaa aag gtt gag aaa acc gtc atc aat gaa aag acg caa tcc gtc aac 912
Lys Lys Val Glu Lys Thr Val Ile Asn Glu Lys Thr Gln Ser Val Asn
290 295 300
agc gcc att gtc ggc aaa ccg gcc tat gaa att gcg aaa atg gca ggc 960
Ser Ala Ile Val Gly Lys Pro Ala Tyr Glu Ile Ala Lys Met Ala Gly
305 310 315 320
gta aat gtt ccg gaa gat acc aaa att tta atc gcc gaa ctt aca ggt 1008
Val Asn Val Pro Glu Asp Thr Lys Ile Leu Ile Ala Glu Leu Thr Gly
325 330 335
gtc ggc ccg gac tac ccg ctt tcc aga gaa aaa tta agc ccg gtt ttg 1056
Val Gly Pro Asp Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Val Leu
340 345 350
gcc tgt tac aaa gcc aac agc aca aaa caa ggt ttc gaa ttt gca gaa 1104
Ala Cys Tyr Lys Ala Asn Ser Thr Lys Gln Gly Phe Glu Phe Ala Glu
355 360 365
gcc atg ctc gaa ttc ggc gga ctt ggt cac tcg gct gtg atc cat tcg 1152
Ala Met Leu Glu Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser
370 375 380
aca aac gat gaa gtc att gaa caa tac ggc tta aaa atg aaa gcc ggc 1200
Thr Asn Asp Glu Val Ile Glu Gln Tyr Gly Leu Lys Met Lys Ala Gly
385 390 395 400
cgg atc att gtc aac tcc ccg tct tcg caa ggg gca atc ggc gac att 1248
Arg Ile Ile Val Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile
405 410 415
tac aat gcc tac atg cct tct ttg aca ctc ggc tgc ggc acg ttt ggc 1296
Tyr Asn Ala Tyr Met Pro Ser Leu Thr Leu Gly Cys Gly Thr Phe Gly
420 425 430
ggc aac tcg gtg tcc aca aac gtc ggt gtc atc aat ctt tac aat gta 1344
Gly Asn Ser Val Ser Thr Asn Val Gly Val Ile Asn Leu Tyr Asn Val
435 440 445
aaa aca atg gcg aaa cgg aga gtc aat atg caa tgg ttt aaa att cca 1392
Lys Thr Met Ala Lys Arg Arg Val Asn Met Gln Trp Phe Lys Ile Pro
450 455 460
ccg cgc gtg tat ttt gaa aag aac tcg gtc caa tac ctg gaa aaa atg 1440
Pro Arg Val Tyr Phe Glu Lys Asn Ser Val Gln Tyr Leu Glu Lys Met
465 470 475 480
ccg gat atc tca agt gcc ttt att gtc acc gat ccg gat atg gtc agg 1488
Pro Asp Ile Ser Ser Ala Phe Ile Val Thr Asp Pro Asp Met Val Arg
485 490 495
ctt gga ttc gtt gag aaa gta ctg tat tac ttg cgg aaa cgc ccg gac 1536
Leu Gly Phe Val Glu Lys Val Leu Tyr Tyr Leu Arg Lys Arg Pro Asp
500 505 510
tat gtg cac tgt gaa att ttc tca gaa gtc gaa ccg gat ccg tct att 1584
Tyr Val His Cys Glu Ile Phe Ser Glu Val Glu Pro Asp Pro Ser Ile
515 520 525
gaa acg gtt cgg aaa ggt gcc gca gct atg gca agc ttc cag ccg gat 1632
Glu Thr Val Arg Lys Gly Ala Ala Ala Met Ala Ser Phe Gln Pro Asp
530 535 540
gtc att atc gcg ctt ggc ggc ggg tcg gcg atg gat gct gca aaa ggc 1680
Val Ile Ile Ala Leu Gly Gly Gly Ser Ala Met Asp Ala Ala Lys Gly
545 550 555 560
atg tgg ctg ttc tat gag cat ccg gaa gtt gat ttc aac gaa ttg aag 1728
Met Trp Leu Phe Tyr Glu His Pro Glu Val Asp Phe Asn Glu Leu Lys
565 570 575
caa aaa ttt atg gac atc cgg aaa cgg gtt gcc aaa ttc ccg aaa ctg 1776
Gln Lys Phe Met Asp Ile Arg Lys Arg Val Ala Lys Phe Pro Lys Leu
580 585 590
ggt gaa aaa gcg cag ctg gtc tgc att ccg acc aca tcg ggc acc ggt 1824
Gly Glu Lys Ala Gln Leu Val Cys Ile Pro Thr Thr Ser Gly Thr Gly
595 600 605
tct gaa gtg act tct ttc gcc gtc att tcg gat aaa aag aac aac aca 1872
Ser Glu Val Thr Ser Phe Ala Val Ile Ser Asp Lys Lys Asn Asn Thr
610 615 620
aaa tat ccg ctc gca gac tat gaa ctg aca ccg gat gtt gcg atc att 1920
Lys Tyr Pro Leu Ala Asp Tyr Glu Leu Thr Pro Asp Val Ala Ile Ile
625 630 635 640
gac ccg cag ttt gtt atg acc gtt ccg aaa tcc gta aca gcc gat acc 1968
Asp Pro Gln Phe Val Met Thr Val Pro Lys Ser Val Thr Ala Asp Thr
645 650 655
ggg atg gac gtt ttg acc cat gcg att gaa gcc tat gta tcc aac atg 2016
Gly Met Asp Val Leu Thr His Ala Ile Glu Ala Tyr Val Ser Asn Met
660 665 670
gcc aac gac tat acg gac ggc ctg gca att aag gcc atc cag ctt gtt 2064
Ala Asn Asp Tyr Thr Asp Gly Leu Ala Ile Lys Ala Ile Gln Leu Val
675 680 685
cac gaa tac ttg ccg aaa gca tac gca aat ggc aat gat gca ctg gca 2112
His Glu Tyr Leu Pro Lys Ala Tyr Ala Asn Gly Asn Asp Ala Leu Ala
690 695 700
cgc gaa aaa atg cac aat gca tcg act ttg gca ggg atg gcg ttc tcg 2160
Arg Glu Lys Met His Asn Ala Ser Thr Leu Ala Gly Met Ala Phe Ser
705 710 715 720
aat gca ttc ctt ggc atc aac cat tca ctt gcc cat aaa atc ggc gct 2208
Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His Lys Ile Gly Ala
725 730 735
gaa ttc cat att ccg cac ggc cgg gct aac gcg att ctg ttg ccg cat 2256
Glu Phe His Ile Pro His Gly Arg Ala Asn Ala Ile Leu Leu Pro His
740 745 750
gtc atc cgc tat aac gcg caa aag ccg aaa aaa ttt gcg gca ttc ccg 2304
Val Ile Arg Tyr Asn Ala Gln Lys Pro Lys Lys Phe Ala Ala Phe Pro
755 760 765
aaa tac gaa cat ttt att gcg gat caa cgc tac gcg gaa atc gcg cgc 2352
Lys Tyr Glu His Phe Ile Ala Asp Gln Arg Tyr Ala Glu Ile Ala Arg
770 775 780
gta ctt ggt ctt ccg gca agc acg aca gaa gaa ggt gtc gaa agc ctt 2400
Val Leu Gly Leu Pro Ala Ser Thr Thr Glu Glu Gly Val Glu Ser Leu
785 790 795 800
tgc cag gaa atc atc cgg atg tgc aaa ctg ttc aat att ccg ctt agc 2448
Cys Gln Glu Ile Ile Arg Met Cys Lys Leu Phe Asn Ile Pro Leu Ser
805 810 815
cta aaa gca gcc ggc gtg aac cgg gcg gac ttt gaa aaa cgg gtt gcc 2496
Leu Lys Ala Ala Gly Val Asn Arg Ala Asp Phe Glu Lys Arg Val Ala
820 825 830
att att gca gac cgc gca ttt gaa gac caa tgc aca cct gca aac cct 2544
Ile Ile Ala Asp Arg Ala Phe Glu Asp Gln Cys Thr Pro Ala Asn Pro
835 840 845
aaa ttg ccg ctt gtc agc gaa ttg gaa gac att tta cgc aaa gct ttt 2592
Lys Leu Pro Leu Val Ser Glu Leu Glu Asp Ile Leu Arg Lys Ala Phe
850 855 860
gaa ggc gtt gaa gca aaa taa 2613
Glu Gly Val Glu Ala Lys
865 870
<210> 78
<211> 870
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 78
Met Ala Ile Asp Glu Lys Val Val Gly Lys Ser Ala Gln Ile Thr Glu
1 5 10 15
Met Val Asp Ser Leu Val Ala Lys Gly Gln Lys Ala Leu Arg Glu Phe
20 25 30
Met Glu Leu Asp Gln Ala Gln Val Asp Asn Ile Val Lys Gln Met Ala
35 40 45
Leu Ala Gly Leu Glu Gln His Met Val Leu Ala Lys Met Ala Val Glu
50 55 60
Glu Thr Gly Arg Gly Val Tyr Glu Asp Lys Met Thr Lys Asn Leu Phe
65 70 75 80
Ala Thr Glu Tyr Ile Tyr His Asn Ile Lys Tyr Asn Lys Thr Val Gly
85 90 95
Ile Ile Asp Glu Asn Asn Asn Glu Gly Ile Val Lys Phe Ala Glu Pro
100 105 110
Val Gly Val Ile Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr
115 120 125
Thr Met Phe Lys Ala Leu Ile Ala Ile Lys Thr Arg Asn Pro Ile Ile
130 135 140
Phe Ala Phe His Pro Ser Ala Gln Lys Cys Ser Ser His Ala Ala Lys
145 150 155 160
Val Met Leu Asp Ala Ala Val Lys Ala Gly Ala Pro Glu Asn Cys Ile
165 170 175
Gln Trp Ile Glu Lys Pro Ala Ile Glu Ala Thr Gln Gln Leu Met Asn
180 185 190
His Ser Gly Ile Ser Leu Ile Leu Ala Thr Gly Gly Ser Gly Met Val
195 200 205
Lys Ser Ala Tyr Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly
210 215 220
Asn Val Pro Cys Tyr Ile Glu Lys Ser Ala Asp Ile Lys Arg Ala Val
225 230 235 240
Asn Asp Leu Ile Leu Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala
245 250 255
Ser Glu Gln Ala Val Ile Ile Asp Lys Glu Ile Tyr Asp Asn Val Lys
260 265 270
Asn Glu Leu Ile Ala Asn Gln Cys Tyr Phe Leu Asn Glu Ala Glu Lys
275 280 285
Lys Lys Val Glu Lys Thr Val Ile Asn Glu Lys Thr Gln Ser Val Asn
290 295 300
Ser Ala Ile Val Gly Lys Pro Ala Tyr Glu Ile Ala Lys Met Ala Gly
305 310 315 320
Val Asn Val Pro Glu Asp Thr Lys Ile Leu Ile Ala Glu Leu Thr Gly
325 330 335
Val Gly Pro Asp Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Val Leu
340 345 350
Ala Cys Tyr Lys Ala Asn Ser Thr Lys Gln Gly Phe Glu Phe Ala Glu
355 360 365
Ala Met Leu Glu Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser
370 375 380
Thr Asn Asp Glu Val Ile Glu Gln Tyr Gly Leu Lys Met Lys Ala Gly
385 390 395 400
Arg Ile Ile Val Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile
405 410 415
Tyr Asn Ala Tyr Met Pro Ser Leu Thr Leu Gly Cys Gly Thr Phe Gly
420 425 430
Gly Asn Ser Val Ser Thr Asn Val Gly Val Ile Asn Leu Tyr Asn Val
435 440 445
Lys Thr Met Ala Lys Arg Arg Val Asn Met Gln Trp Phe Lys Ile Pro
450 455 460
Pro Arg Val Tyr Phe Glu Lys Asn Ser Val Gln Tyr Leu Glu Lys Met
465 470 475 480
Pro Asp Ile Ser Ser Ala Phe Ile Val Thr Asp Pro Asp Met Val Arg
485 490 495
Leu Gly Phe Val Glu Lys Val Leu Tyr Tyr Leu Arg Lys Arg Pro Asp
500 505 510
Tyr Val His Cys Glu Ile Phe Ser Glu Val Glu Pro Asp Pro Ser Ile
515 520 525
Glu Thr Val Arg Lys Gly Ala Ala Ala Met Ala Ser Phe Gln Pro Asp
530 535 540
Val Ile Ile Ala Leu Gly Gly Gly Ser Ala Met Asp Ala Ala Lys Gly
545 550 555 560
Met Trp Leu Phe Tyr Glu His Pro Glu Val Asp Phe Asn Glu Leu Lys
565 570 575
Gln Lys Phe Met Asp Ile Arg Lys Arg Val Ala Lys Phe Pro Lys Leu
580 585 590
Gly Glu Lys Ala Gln Leu Val Cys Ile Pro Thr Thr Ser Gly Thr Gly
595 600 605
Ser Glu Val Thr Ser Phe Ala Val Ile Ser Asp Lys Lys Asn Asn Thr
610 615 620
Lys Tyr Pro Leu Ala Asp Tyr Glu Leu Thr Pro Asp Val Ala Ile Ile
625 630 635 640
Asp Pro Gln Phe Val Met Thr Val Pro Lys Ser Val Thr Ala Asp Thr
645 650 655
Gly Met Asp Val Leu Thr His Ala Ile Glu Ala Tyr Val Ser Asn Met
660 665 670
Ala Asn Asp Tyr Thr Asp Gly Leu Ala Ile Lys Ala Ile Gln Leu Val
675 680 685
His Glu Tyr Leu Pro Lys Ala Tyr Ala Asn Gly Asn Asp Ala Leu Ala
690 695 700
Arg Glu Lys Met His Asn Ala Ser Thr Leu Ala Gly Met Ala Phe Ser
705 710 715 720
Asn Ala Phe Leu Gly Ile Asn His Ser Leu Ala His Lys Ile Gly Ala
725 730 735
Glu Phe His Ile Pro His Gly Arg Ala Asn Ala Ile Leu Leu Pro His
740 745 750
Val Ile Arg Tyr Asn Ala Gln Lys Pro Lys Lys Phe Ala Ala Phe Pro
755 760 765
Lys Tyr Glu His Phe Ile Ala Asp Gln Arg Tyr Ala Glu Ile Ala Arg
770 775 780
Val Leu Gly Leu Pro Ala Ser Thr Thr Glu Glu Gly Val Glu Ser Leu
785 790 795 800
Cys Gln Glu Ile Ile Arg Met Cys Lys Leu Phe Asn Ile Pro Leu Ser
805 810 815
Leu Lys Ala Ala Gly Val Asn Arg Ala Asp Phe Glu Lys Arg Val Ala
820 825 830
Ile Ile Ala Asp Arg Ala Phe Glu Asp Gln Cys Thr Pro Ala Asn Pro
835 840 845
Lys Leu Pro Leu Val Ser Glu Leu Glu Asp Ile Leu Arg Lys Ala Phe
850 855 860
Glu Gly Val Glu Ala Lys
865 870
<210> 79
<211> 711
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(711)
<223> 乳酸乳球菌乳脂亚种 MG1363; 基因 llmg_1275
编码a-乙酰乳酸脱羧酶
<400> 79
atg tca gaa atc aca caa ctt ttt caa tat aat acc ctt ggg gca tta 48
Met Ser Glu Ile Thr Gln Leu Phe Gln Tyr Asn Thr Leu Gly Ala Leu
1 5 10 15
atg gcc gga ctt tat gag ggg aca atg acg att ggt gag ctc ttg aaa 96
Met Ala Gly Leu Tyr Glu Gly Thr Met Thr Ile Gly Glu Leu Leu Lys
20 25 30
cat ggt gac tta gga att gga act tta gat tca att gat ggt gaa ttg 144
His Gly Asp Leu Gly Ile Gly Thr Leu Asp Ser Ile Asp Gly Glu Leu
35 40 45
att gtt tta gat ggt aaa gct tat caa gct aag gga gat aaa acg atc 192
Ile Val Leu Asp Gly Lys Ala Tyr Gln Ala Lys Gly Asp Lys Thr Ile
50 55 60
gtc gaa tta act gac gat atc aaa gtt cct tat gct gca gtt gtt cct 240
Val Glu Leu Thr Asp Asp Ile Lys Val Pro Tyr Ala Ala Val Val Pro
65 70 75 80
cat cag gca gaa gtt gtt ttc aaa caa aaa ttt aca gta agc gat aaa 288
His Gln Ala Glu Val Val Phe Lys Gln Lys Phe Thr Val Ser Asp Lys
85 90 95
gaa ttg gaa gac cga att gaa agc tat ttt gat ggt caa aac tta ttc 336
Glu Leu Glu Asp Arg Ile Glu Ser Tyr Phe Asp Gly Gln Asn Leu Phe
100 105 110
cgc tca atc aaa att act ggt aaa ttt cca aaa atg cat gta cga atg 384
Arg Ser Ile Lys Ile Thr Gly Lys Phe Pro Lys Met His Val Arg Met
115 120 125
att ccg cgt gct aaa tca gga aca aaa ttt gta gaa gtt tca caa aac 432
Ile Pro Arg Ala Lys Ser Gly Thr Lys Phe Val Glu Val Ser Gln Asn
130 135 140
caa cca gaa tat acc gaa gaa aat atc aaa gga aca att gtt gga att 480
Gln Pro Glu Tyr Thr Glu Glu Asn Ile Lys Gly Thr Ile Val Gly Ile
145 150 155 160
tgg act cct gaa atg ttc cat ggt gtc agc gtt gct ggt tat cat ctt 528
Trp Thr Pro Glu Met Phe His Gly Val Ser Val Ala Gly Tyr His Leu
165 170 175
cat ttt att agt gaa gat ttc act ttt ggt gga cat gtt ctt gat ttt 576
His Phe Ile Ser Glu Asp Phe Thr Phe Gly Gly His Val Leu Asp Phe
180 185 190
att att gat aat ggg act gtt gaa att gga gca att gac caa ttg aat 624
Ile Ile Asp Asn Gly Thr Val Glu Ile Gly Ala Ile Asp Gln Leu Asn
195 200 205
caa tca ttc cct gtt caa gat cgc aaa ttt tta ttt gcc gac ctt gac 672
Gln Ser Phe Pro Val Gln Asp Arg Lys Phe Leu Phe Ala Asp Leu Asp
210 215 220
att gag gct ttg aaa aaa gat att gat gta gct gaa tga 771
Ile Glu Ala Leu Lys Lys Asp Ile Asp Val Ala Glu
225 230 235
<210> 80
<211> 236
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 80
Met Ser Glu Ile Thr Gln Leu Phe Gln Tyr Asn Thr Leu Gly Ala Leu
1 5 10 15
Met Ala Gly Leu Tyr Glu Gly Thr Met Thr Ile Gly Glu Leu Leu Lys
20 25 30
His Gly Asp Leu Gly Ile Gly Thr Leu Asp Ser Ile Asp Gly Glu Leu
35 40 45
Ile Val Leu Asp Gly Lys Ala Tyr Gln Ala Lys Gly Asp Lys Thr Ile
50 55 60
Val Glu Leu Thr Asp Asp Ile Lys Val Pro Tyr Ala Ala Val Val Pro
65 70 75 80
His Gln Ala Glu Val Val Phe Lys Gln Lys Phe Thr Val Ser Asp Lys
85 90 95
Glu Leu Glu Asp Arg Ile Glu Ser Tyr Phe Asp Gly Gln Asn Leu Phe
100 105 110
Arg Ser Ile Lys Ile Thr Gly Lys Phe Pro Lys Met His Val Arg Met
115 120 125
Ile Pro Arg Ala Lys Ser Gly Thr Lys Phe Val Glu Val Ser Gln Asn
130 135 140
Gln Pro Glu Tyr Thr Glu Glu Asn Ile Lys Gly Thr Ile Val Gly Ile
145 150 155 160
Trp Thr Pro Glu Met Phe His Gly Val Ser Val Ala Gly Tyr His Leu
165 170 175
His Phe Ile Ser Glu Asp Phe Thr Phe Gly Gly His Val Leu Asp Phe
180 185 190
Ile Ile Asp Asn Gly Thr Val Glu Ile Gly Ala Ile Asp Gln Leu Asn
195 200 205
Gln Ser Phe Pro Val Gln Asp Arg Lys Phe Leu Phe Ala Asp Leu Asp
210 215 220
Ile Glu Ala Leu Lys Lys Asp Ile Asp Val Ala Glu
225 230 235
<210> 81
<211> 711
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(711)
<223> 干酪乳杆菌 ATCC 334;基因 LSEI_1840 编码a-乙酰乳酸脱羧酶
<220>
<221> misc_feature（新的或少见的特征）
<222> (1)..(3)
<223> 由起始密码子（GTG）处的tRNA插入Met而不是Val
<400> 81
gtg aat act gat cgg tta tat caa cat ggc aca ttg gca atg ctt gta 48
Val Asn Thr Asp Arg Leu Tyr Gln His Gly Thr Leu Ala Met Leu Val
1 5 10 15
ccc gga ttg ttt gct ggt acg caa aag att gaa gaa ctg ctg cag cat 96
Pro Gly Leu Phe Ala Gly Thr Gln Lys Ile Glu Glu Leu Leu Gln His
20 25 30
ggc aat act ggt att ggc act ttg acg gcg cta gat ggc gaa ctt gtg 144
Gly Asn Thr Gly Ile Gly Thr Leu Thr Ala Leu Asp Gly Glu Leu Val
35 40 45
atc att gat agc aaa gtt tat caa gtc aat gca caa ggc gct gtt cgc 192
Ile Ile Asp Ser Lys Val Tyr Gln Val Asn Ala Gln Gly Ala Val Arg
50 55 60
gaa gtc ggg tca gaa gaa gaa gta ccg ttt gca aac gtg cat tat caa 240
Glu Val Gly Ser Glu Glu Glu Val Pro Phe Ala Asn Val His Tyr Gln
65 70 75 80
gct gat aaa tct gta ggc aaa cta caa gga ctt gat ttg tca ggc ttt 288
Ala Asp Lys Ser Val Gly Lys Leu Gln Gly Leu Asp Leu Ser Gly Phe
85 90 95
caa aaa acg gtt ttg gaa cgc ctt caa acg gcc aat ctt ttt gct gca 336
Gln Lys Thr Val Leu Glu Arg Leu Gln Thr Ala Asn Leu Phe Ala Ala
100 105 110
gta cgc gtt gag ggg cgg ttt acg cag atg cat aca cgc gcg gtg ttg 384
Val Arg Val Glu Gly Arg Phe Thr Gln Met His Thr Arg Ala Val Leu
115 120 125
cca caa cag cca ccg tat cca acg ctt act gaa aca gct tca ggt caa 432
Pro Gln Gln Pro Pro Tyr Pro Thr Leu Thr Glu Thr Ala Ser Gly Gln
130 135 140
aaa gaa ttc aac gct gag aat atc aag ggt aca ttg att ggg tat tat 480
Lys Glu Phe Asn Ala Glu Asn Ile Lys Gly Thr Leu Ile Gly Tyr Tyr
145 150 155 160
tcg cct gat ctc tat gcg ggt gcg gtg tca ccg ggg ttc cac ttg cat 528
Ser Pro Asp Leu Tyr Ala Gly Ala Val Ser Pro Gly Phe His Leu His
165 170 175
ttt ctt gac gct gat cat cac atg ggt ggc cat att ctt ggc ttc gaa 576
Phe Leu Asp Ala Asp His His Met Gly Gly His Ile Leu Gly Phe Glu
180 185 190
ctc gac agt ggc gaa ttg ttt ctg caa aag ttt agt gac ttc cag cta 624
Leu Asp Ser Gly Glu Leu Phe Leu Gln Lys Phe Ser Asp Phe Gln Leu
195 200 205
cat ctg ccg aca acc aac gat gct ttt tta aag caa aaa ttt gac acg 672
His Leu Pro Thr Thr Asn Asp Ala Phe Leu Lys Gln Lys Phe Asp Thr
210 215 220
gca aca ctg gta gcc gat att cgc aag gct gag aat tga 711
Ala Thr Leu Val Ala Asp Ile Arg Lys Ala Glu Asn
225 230 235
<210> 82
<211> 236
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 82
Val Asn Thr Asp Arg Leu Tyr Gln His Gly Thr Leu Ala Met Leu Val
1 5 10 15
Pro Gly Leu Phe Ala Gly Thr Gln Lys Ile Glu Glu Leu Leu Gln His
20 25 30
Gly Asn Thr Gly Ile Gly Thr Leu Thr Ala Leu Asp Gly Glu Leu Val
35 40 45
Ile Ile Asp Ser Lys Val Tyr Gln Val Asn Ala Gln Gly Ala Val Arg
50 55 60
Glu Val Gly Ser Glu Glu Glu Val Pro Phe Ala Asn Val His Tyr Gln
65 70 75 80
Ala Asp Lys Ser Val Gly Lys Leu Gln Gly Leu Asp Leu Ser Gly Phe
85 90 95
Gln Lys Thr Val Leu Glu Arg Leu Gln Thr Ala Asn Leu Phe Ala Ala
100 105 110
Val Arg Val Glu Gly Arg Phe Thr Gln Met His Thr Arg Ala Val Leu
115 120 125
Pro Gln Gln Pro Pro Tyr Pro Thr Leu Thr Glu Thr Ala Ser Gly Gln
130 135 140
Lys Glu Phe Asn Ala Glu Asn Ile Lys Gly Thr Leu Ile Gly Tyr Tyr
145 150 155 160
Ser Pro Asp Leu Tyr Ala Gly Ala Val Ser Pro Gly Phe His Leu His
165 170 175
Phe Leu Asp Ala Asp His His Met Gly Gly His Ile Leu Gly Phe Glu
180 185 190
Leu Asp Ser Gly Glu Leu Phe Leu Gln Lys Phe Ser Asp Phe Gln Leu
195 200 205
His Leu Pro Thr Thr Asn Asp Ala Phe Leu Lys Gln Lys Phe Asp Thr
210 215 220
Ala Thr Leu Val Ala Asp Ile Arg Lys Ala Glu Asn
225 230 235
<210> 83
<211> 711
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(711)
<223> 植物乳杆菌 WCFS1; 基因 lp_2030 编码a-乙酰乳酸脱羧酶
<400> 83
atg gac aca aca aca att ttt caa cac ggc aca tta ggc tta ctt gtt 48
Met Asp Thr Thr Thr Ile Phe Gln His Gly Thr Leu Gly Leu Leu Val
1 5 10 15
ccc ggc tta ttt gac ggg acg att acg gct ggt gaa ctc tta acc cat 96
Pro Gly Leu Phe Asp Gly Thr Ile Thr Ala Gly Glu Leu Leu Thr His
20 25 30
ggt gat acg ggt att ggg acg tta gac ggt ctc aat ggt gaa gtg att 144
Gly Asp Thr Gly Ile Gly Thr Leu Asp Gly Leu Asn Gly Glu Val Ile
35 40 45
att cta gga ggt cac gcc tat caa gca cgt gag gat ggt caa att cgt 192
Ile Leu Gly Gly His Ala Tyr Gln Ala Arg Glu Asp Gly Gln Ile Arg
50 55 60
gaa att cag ccc gaa gag acg tta cca ttt gca tcg gtg cac ttt gaa 240
Glu Ile Gln Pro Glu Glu Thr Leu Pro Phe Ala Ser Val His Phe Glu
65 70 75 80
aag cct gat att agt gcg caa tta gct gca atc aca caa act gat ttt 288
Lys Pro Asp Ile Ser Ala Gln Leu Ala Ala Ile Thr Gln Thr Asp Phe
85 90 95
gag caa caa gtg gtt cat gac tat cgt ttg acc aac gtg ttt gca gct 336
Glu Gln Gln Val Val His Asp Tyr Arg Leu Thr Asn Val Phe Ala Ala
100 105 110
att cgc gtc gat ggg acc ttt gca aaa gtc aag acg cga gtc gcg cct 384
Ile Arg Val Asp Gly Thr Phe Ala Lys Val Lys Thr Arg Val Ala Pro
115 120 125
cgt cag gaa cca ccg tac aaa aca tta gtc gcg gca acg gca aca caa 432
Arg Gln Glu Pro Pro Tyr Lys Thr Leu Val Ala Ala Thr Ala Thr Gln
130 135 140
ccg gaa ttc acc ggt gaa cat gtt gac gga acg att att ggc tac tac 480
Pro Glu Phe Thr Gly Glu His Val Asp Gly Thr Ile Ile Gly Tyr Tyr
145 150 155 160
gca ccg cat ttg ttc caa ggc gct acg gtc ggt ggc ttt cac tta cac 528
Ala Pro His Leu Phe Gln Gly Ala Thr Val Gly Gly Phe His Leu His
165 170 175
ttt cta agt aaa gac cat caa tta ggt gga cac ttg ctg ggg ttt gaa 576
Phe Leu Ser Lys Asp His Gln Leu Gly Gly His Leu Leu Gly Phe Glu
180 185 190
gtc gaa caa gcg acg ctg aaa gtt caa cat ttt gct gac ttt cat gtg 624
Val Glu Gln Ala Thr Leu Lys Val Gln His Phe Ala Asp Phe His Val
195 200 205
cac ttg cca atc gac aat gaa gcg tac tta caa gaa caa ttt gat aat 672
His Leu Pro Ile Asp Asn Glu Ala Tyr Leu Gln Glu Gln Phe Asp Asn
210 215 220
gaa gcc att gat cat gcc att aat aaa gct gag cgt tga 711
Glu Ala Ile Asp His Ala Ile Asn Lys Ala Glu Arg
225 230 235
<210> 84
<211> 236
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 84
Met Asp Thr Thr Thr Ile Phe Gln His Gly Thr Leu Gly Leu Leu Val
1 5 10 15
Pro Gly Leu Phe Asp Gly Thr Ile Thr Ala Gly Glu Leu Leu Thr His
20 25 30
Gly Asp Thr Gly Ile Gly Thr Leu Asp Gly Leu Asn Gly Glu Val Ile
35 40 45
Ile Leu Gly Gly His Ala Tyr Gln Ala Arg Glu Asp Gly Gln Ile Arg
50 55 60
Glu Ile Gln Pro Glu Glu Thr Leu Pro Phe Ala Ser Val His Phe Glu
65 70 75 80
Lys Pro Asp Ile Ser Ala Gln Leu Ala Ala Ile Thr Gln Thr Asp Phe
85 90 95
Glu Gln Gln Val Val His Asp Tyr Arg Leu Thr Asn Val Phe Ala Ala
100 105 110
Ile Arg Val Asp Gly Thr Phe Ala Lys Val Lys Thr Arg Val Ala Pro
115 120 125
Arg Gln Glu Pro Pro Tyr Lys Thr Leu Val Ala Ala Thr Ala Thr Gln
130 135 140
Pro Glu Phe Thr Gly Glu His Val Asp Gly Thr Ile Ile Gly Tyr Tyr
145 150 155 160
Ala Pro His Leu Phe Gln Gly Ala Thr Val Gly Gly Phe His Leu His
165 170 175
Phe Leu Ser Lys Asp His Gln Leu Gly Gly His Leu Leu Gly Phe Glu
180 185 190
Val Glu Gln Ala Thr Leu Lys Val Gln His Phe Ala Asp Phe His Val
195 200 205
His Leu Pro Ile Asp Asn Glu Ala Tyr Leu Gln Glu Gln Phe Asp Asn
210 215 220
Glu Ala Ile Asp His Ala Ile Asn Lys Ala Glu Arg
225 230 235
<210> 85
<211> 705
<212> DNA
<213> 戊糖片球菌（Pediococcus pentosaceus）
<220>
<221> CDS
<222> (1)..(705)
<223> 戊糖片球菌 ATCC 25745; 基因 PEPE_0406 编码a-乙酰乳酸脱羧酶
<400> 85
atg gca aaa tta ttt caa cat ggg acg ctc gca atg cta gta gat gga 48
Met Ala Lys Leu Phe Gln His Gly Thr Leu Ala Met Leu Val Asp Gly
1 5 10 15
ctt ttt ggt ggg acg ctt gcg gtt tcc gat tta ctg aag cac ggt gac 96
Leu Phe Gly Gly Thr Leu Ala Val Ser Asp Leu Leu Lys His Gly Asp
20 25 30
ttt gga atc gga act gct gaa ggc ctt aat ggc gaa tta att att tta 144
Phe Gly Ile Gly Thr Ala Glu Gly Leu Asn Gly Glu Leu Ile Ile Leu
35 40 45
gat gga gcc cct tac caa gcg cta gca gac ggt aca att agg gtt att 192
Asp Gly Ala Pro Tyr Gln Ala Leu Ala Asp Gly Thr Ile Arg Val Ile
50 55 60
ggt gat gat gaa cta tta ccg ttt gct aac gtt aat tca gcg gat ttt 240
Gly Asp Asp Glu Leu Leu Pro Phe Ala Asn Val Asn Ser Ala Asp Phe
65 70 75 80
gaa gga cga gca gtg tta tct gac att gag atg aaa gat gtg gat gcg 288
Glu Gly Arg Ala Val Leu Ser Asp Ile Glu Met Lys Asp Val Asp Ala
85 90 95
aca ctt gct aaa gaa ttg tca tat caa aat aca ttt gtt gct att aaa 336
Thr Leu Ala Lys Glu Leu Ser Tyr Gln Asn Thr Phe Val Ala Ile Lys
100 105 110
att agt gga act ttc cgt aca gtt caa act cgg gtg gta aag caa caa 384
Ile Ser Gly Thr Phe Arg Thr Val Gln Thr Arg Val Val Lys Gln Gln
115 120 125
gaa cgt cct tat cca acc ctt agt gaa aca gca tct aaa caa caa gtt 432
Glu Arg Pro Tyr Pro Thr Leu Ser Glu Thr Ala Ser Lys Gln Gln Val
130 135 140
ttt aat gct gaa gat gtt aga gga aca gta gtt ggc tac tac acc cca 480
Phe Asn Ala Glu Asp Val Arg Gly Thr Val Val Gly Tyr Tyr Thr Pro
145 150 155 160
gat ctt ttc cat ggt gcg gga gta gcg ggg atg cat ctt cat ttt att 528
Asp Leu Phe His Gly Ala Gly Val Ala Gly Met His Leu His Phe Ile
165 170 175
gac gat caa cac gaa ttc gga ggt cat tta cta gac ttt aag gcg gat 576
Asp Asp Gln His Glu Phe Gly Gly His Leu Leu Asp Phe Lys Ala Asp
180 185 190
caa gtt aaa ctt tct tgg caa cta tta gac ggt tta gac ctc aat tta 624
Gln Val Lys Leu Ser Trp Gln Leu Leu Asp Gly Leu Asp Leu Asn Leu
195 200 205
cca att caa gac gca gaa ttt atg gca cat gag gct agc gac gcg gaa 672
Pro Ile Gln Asp Ala Glu Phe Met Ala His Glu Ala Ser Asp Ala Glu
210 215 220
aaa att caa caa tca att tct gag tct gaa taa 705
Lys Ile Gln Gln Ser Ile Ser Glu Ser Glu
225 230
<210> 86
<211> 234
<212> PRT
<213> 戊糖片球菌（Pediococcus pentosaceus）
<400> 86
Met Ala Lys Leu Phe Gln His Gly Thr Leu Ala Met Leu Val Asp Gly
1 5 10 15
Leu Phe Gly Gly Thr Leu Ala Val Ser Asp Leu Leu Lys His Gly Asp
20 25 30
Phe Gly Ile Gly Thr Ala Glu Gly Leu Asn Gly Glu Leu Ile Ile Leu
35 40 45
Asp Gly Ala Pro Tyr Gln Ala Leu Ala Asp Gly Thr Ile Arg Val Ile
50 55 60
Gly Asp Asp Glu Leu Leu Pro Phe Ala Asn Val Asn Ser Ala Asp Phe
65 70 75 80
Glu Gly Arg Ala Val Leu Ser Asp Ile Glu Met Lys Asp Val Asp Ala
85 90 95
Thr Leu Ala Lys Glu Leu Ser Tyr Gln Asn Thr Phe Val Ala Ile Lys
100 105 110
Ile Ser Gly Thr Phe Arg Thr Val Gln Thr Arg Val Val Lys Gln Gln
115 120 125
Glu Arg Pro Tyr Pro Thr Leu Ser Glu Thr Ala Ser Lys Gln Gln Val
130 135 140
Phe Asn Ala Glu Asp Val Arg Gly Thr Val Val Gly Tyr Tyr Thr Pro
145 150 155 160
Asp Leu Phe His Gly Ala Gly Val Ala Gly Met His Leu His Phe Ile
165 170 175
Asp Asp Gln His Glu Phe Gly Gly His Leu Leu Asp Phe Lys Ala Asp
180 185 190
Gln Val Lys Leu Ser Trp Gln Leu Leu Asp Gly Leu Asp Leu Asn Leu
195 200 205
Pro Ile Gln Asp Ala Glu Phe Met Ala His Glu Ala Ser Asp Ala Glu
210 215 220
Lys Ile Gln Gln Ser Ile Ser Glu Ser Glu
225 230
<210> 87
<211> 720
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(720)
<223> 肠膜明串珠菌肠膜亚种 ATCC 8293; 基因
LEUM_0549 编码a-乙酰乳酸脱羧酶
<400> 87
atg aca aca ata tat caa cat ggt aca tta gca caa tta gta gcg cgc 48
Met Thr Thr Ile Tyr Gln His Gly Thr Leu Ala Gln Leu Val Ala Arg
1 5 10 15
caa atg tca ggg aca ata aca gtc gct gaa atg ttg gaa cat ggg gac 96
Gln Met Ser Gly Thr Ile Thr Val Ala Glu Met Leu Glu His Gly Asp
20 25 30
act ggc att ggt act ttt gag ggc ctc aat ggt gaa gct att ttt cta 144
Thr Gly Ile Gly Thr Phe Glu Gly Leu Asn Gly Glu Ala Ile Phe Leu
35 40 45
aac ggg gaa gcc tat caa gct gat agt aca gga aaa gtt cac cac att 192
Asn Gly Glu Ala Tyr Gln Ala Asp Ser Thr Gly Lys Val His His Ile
50 55 60
act gat aaa caa act aca tta cct ttt gca tca atc cat ttt gat caa 240
Thr Asp Lys Gln Thr Thr Leu Pro Phe Ala Ser Ile His Phe Asp Gln
65 70 75 80
ccg gag gca agc caa aaa tta cct ttt aaa aaa ata aaa tat agt aat 288
Pro Glu Ala Ser Gln Lys Leu Pro Phe Lys Lys Ile Lys Tyr Ser Asn
85 90 95
ttg act cag aac ttg aaa gat gag cag tta ttt aac gtt ttc tct gcc 336
Leu Thr Gln Asn Leu Lys Asp Glu Gln Leu Phe Asn Val Phe Ser Ala
100 105 110
tta aaa ctg cat ggt gag ttt gcc cac gtt cac gtt cgt att gta aca 384
Leu Lys Leu His Gly Glu Phe Ala His Val His Val Arg Ile Val Thr
115 120 125
aaa caa gag aag cca tat cca agt ttg tta caa gta gct gaa cag cag 432
Lys Gln Glu Lys Pro Tyr Pro Ser Leu Leu Gln Val Ala Glu Gln Gln
130 135 140
cct gaa ttc aaa gca gac aac ata act ggt aca tta gtt gga tat tat 480
Pro Glu Phe Lys Ala Asp Asn Ile Thr Gly Thr Leu Val Gly Tyr Tyr
145 150 155 160
gca cca aaa gtt ttt ggc ggt cca acc gca gca ggg tgg cat tta cac 528
Ala Pro Lys Val Phe Gly Gly Pro Thr Ala Ala Gly Trp His Leu His
165 170 175
ttt ttg tca gat gat tta acc ttt gct ggg cac gtt ttg gat ttt gaa 576
Phe Leu Ser Asp Asp Leu Thr Phe Ala Gly His Val Leu Asp Phe Glu
180 185 190
gca aca gat gtc gat ggt act tta gaa gtt ttt gat aac ttt ttg caa 624
Ala Thr Asp Val Asp Gly Thr Leu Glu Val Phe Asp Asn Phe Leu Gln
195 200 205
cat cta cct att aat aat gct gac ttt aga agc atg aat cag gat ata 672
His Leu Pro Ile Asn Asn Ala Asp Phe Arg Ser Met Asn Gln Asp Ile
210 215 220
gct ggt ttg gat aaa gcc att gag gcc agt gaa ggc gga aaa aat tag 720
Ala Gly Leu Asp Lys Ala Ile Glu Ala Ser Glu Gly Gly Lys Asn
225 230 235
<210> 88
<211> 239
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 88
Met Thr Thr Ile Tyr Gln His Gly Thr Leu Ala Gln Leu Val Ala Arg
1 5 10 15
Gln Met Ser Gly Thr Ile Thr Val Ala Glu Met Leu Glu His Gly Asp
20 25 30
Thr Gly Ile Gly Thr Phe Glu Gly Leu Asn Gly Glu Ala Ile Phe Leu
35 40 45
Asn Gly Glu Ala Tyr Gln Ala Asp Ser Thr Gly Lys Val His His Ile
50 55 60
Thr Asp Lys Gln Thr Thr Leu Pro Phe Ala Ser Ile His Phe Asp Gln
65 70 75 80
Pro Glu Ala Ser Gln Lys Leu Pro Phe Lys Lys Ile Lys Tyr Ser Asn
85 90 95
Leu Thr Gln Asn Leu Lys Asp Glu Gln Leu Phe Asn Val Phe Ser Ala
100 105 110
Leu Lys Leu His Gly Glu Phe Ala His Val His Val Arg Ile Val Thr
115 120 125
Lys Gln Glu Lys Pro Tyr Pro Ser Leu Leu Gln Val Ala Glu Gln Gln
130 135 140
Pro Glu Phe Lys Ala Asp Asn Ile Thr Gly Thr Leu Val Gly Tyr Tyr
145 150 155 160
Ala Pro Lys Val Phe Gly Gly Pro Thr Ala Ala Gly Trp His Leu His
165 170 175
Phe Leu Ser Asp Asp Leu Thr Phe Ala Gly His Val Leu Asp Phe Glu
180 185 190
Ala Thr Asp Val Asp Gly Thr Leu Glu Val Phe Asp Asn Phe Leu Gln
195 200 205
His Leu Pro Ile Asn Asn Ala Asp Phe Arg Ser Met Asn Gln Asp Ile
210 215 220
Ala Gly Leu Asp Lys Ala Ile Glu Ala Ser Glu Gly Gly Lys Asn
225 230 235
<210> 89
<211> 711
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(711)
<223> 肠膜明串珠菌肠膜亚种 ATCC 8293; 基因
LEUM_0954 编码a-乙酰乳酸脱羧酶
<400> 89
atg tct aca att tat caa cat gga acg tta gaa tta att atg tca gga 48
Met Ser Thr Ile Tyr Gln His Gly Thr Leu Glu Leu Ile Met Ser Gly
1 5 10 15
tta tat gac ggt acg gca ccg ttg tcg tca ata ttg aat cat ggt tcg 96
Leu Tyr Asp Gly Thr Ala Pro Leu Ser Ser Ile Leu Asn His Gly Ser
20 25 30
agt ggc att gga aca atg gat gaa ctt gat ggt gaa gtt aca atc ata 144
Ser Gly Ile Gly Thr Met Asp Glu Leu Asp Gly Glu Val Thr Ile Ile
35 40 45
gaa aat agc gta tat caa acg aaa gcg aat ggt gta act aaa gga atc 192
Glu Asn Ser Val Tyr Gln Thr Lys Ala Asn Gly Val Thr Lys Gly Ile
50 55 60
aca gat tta tcg ttg gaa aca cct ttt act tcc atc cat tct caa tca 240
Thr Asp Leu Ser Leu Glu Thr Pro Phe Thr Ser Ile His Ser Gln Ser
65 70 75 80
gag tta tac tat ttt ctt caa gaa aaa ttt tta act ttt gat agt tta 288
Glu Leu Tyr Tyr Phe Leu Gln Glu Lys Phe Leu Thr Phe Asp Ser Leu
85 90 95
aac aat ata tcc aaa caa cat cag agc tgg gct aat tta cca gct agt 336
Asn Asn Ile Ser Lys Gln His Gln Ser Trp Ala Asn Leu Pro Ala Ser
100 105 110
att caa ata aat gct agt ttt tcg aaa ctg aag gtt cgt gtt gct cct 384
Ile Gln Ile Asn Ala Ser Phe Ser Lys Leu Lys Val Arg Val Ala Pro
115 120 125
aag caa atg aag cct tat cca ccc ttc gtt gat gta aca ttg aaa caa 432
Lys Gln Met Lys Pro Tyr Pro Pro Phe Val Asp Val Thr Leu Lys Gln
130 135 140
cca gtg ttt aat tat gaa aat gtt tct ggt act tta att ggt gat ttt 480
Pro Val Phe Asn Tyr Glu Asn Val Ser Gly Thr Leu Ile Gly Asp Phe
145 150 155 160
ggt cct gac ctt tat tct ggc att atg gcg agt ggg tgg cat ctt cat 528
Gly Pro Asp Leu Tyr Ser Gly Ile Met Ala Ser Gly Trp His Leu His
165 170 175
ttt tta agt gat gat cgt acg att ggc ggc cat gtt ctt gat ttc aag 576
Phe Leu Ser Asp Asp Arg Thr Ile Gly Gly His Val Leu Asp Phe Lys
180 185 190
gcg agc gat gta tta ggt aga att gat tta ttc aat gaa ctc aac atc 624
Ala Ser Asp Val Leu Gly Arg Ile Asp Leu Phe Asn Glu Leu Asn Ile
195 200 205
gct ttg cct act gat aat cct gat ttc aat aat cat gaa gcc gac ata 672
Ala Leu Pro Thr Asp Asn Pro Asp Phe Asn Asn His Glu Ala Asp Ile
210 215 220
ttg aat att cgt gaa gcc atc gat gta gca gaa aga taa 711
Leu Asn Ile Arg Glu Ala Ile Asp Val Ala Glu Arg
225 230 235
<210> 90
<211> 236
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 90
Met Ser Thr Ile Tyr Gln His Gly Thr Leu Glu Leu Ile Met Ser Gly
1 5 10 15
Leu Tyr Asp Gly Thr Ala Pro Leu Ser Ser Ile Leu Asn His Gly Ser
20 25 30
Ser Gly Ile Gly Thr Met Asp Glu Leu Asp Gly Glu Val Thr Ile Ile
35 40 45
Glu Asn Ser Val Tyr Gln Thr Lys Ala Asn Gly Val Thr Lys Gly Ile
50 55 60
Thr Asp Leu Ser Leu Glu Thr Pro Phe Thr Ser Ile His Ser Gln Ser
65 70 75 80
Glu Leu Tyr Tyr Phe Leu Gln Glu Lys Phe Leu Thr Phe Asp Ser Leu
85 90 95
Asn Asn Ile Ser Lys Gln His Gln Ser Trp Ala Asn Leu Pro Ala Ser
100 105 110
Ile Gln Ile Asn Ala Ser Phe Ser Lys Leu Lys Val Arg Val Ala Pro
115 120 125
Lys Gln Met Lys Pro Tyr Pro Pro Phe Val Asp Val Thr Leu Lys Gln
130 135 140
Pro Val Phe Asn Tyr Glu Asn Val Ser Gly Thr Leu Ile Gly Asp Phe
145 150 155 160
Gly Pro Asp Leu Tyr Ser Gly Ile Met Ala Ser Gly Trp His Leu His
165 170 175
Phe Leu Ser Asp Asp Arg Thr Ile Gly Gly His Val Leu Asp Phe Lys
180 185 190
Ala Ser Asp Val Leu Gly Arg Ile Asp Leu Phe Asn Glu Leu Asn Ile
195 200 205
Ala Leu Pro Thr Asp Asn Pro Asp Phe Asn Asn His Glu Ala Asp Ile
210 215 220
Leu Asn Ile Arg Glu Ala Ile Asp Val Ala Glu Arg
225 230 235
<210> 91
<211> 720
<212> DNA
<213> 嗜热链球菌（Streptococcus thermophilus）
<220>
<221> CDS
<222> (1)..(720)
<223> 嗜热链球菌 CNRZ1066; 基因 str0924 编码a-乙酰乳酸脱羧酶
<400> 91
atg tct gaa gcc ata aaa tta ttt caa tat aat act ttg gga gcc ttg 48
Met Ser Glu Ala Ile Lys Leu Phe Gln Tyr Asn Thr Leu Gly Ala Leu
1 5 10 15
atg gct ggg ctt tat gga ggt act tta acc gtt gga gag ttg tta gag 96
Met Ala Gly Leu Tyr Gly Gly Thr Leu Thr Val Gly Glu Leu Leu Glu
20 25 30
cat gga gac ttg ggt ctc gga aca tta gat tct atc gat ggt gag ttg 144
His Gly Asp Leu Gly Leu Gly Thr Leu Asp Ser Ile Asp Gly Glu Leu
35 40 45
att gtt ctt gac ggt aaa gcc tat caa gct aaa ggc tct gaa ggt aaa 192
Ile Val Leu Asp Gly Lys Ala Tyr Gln Ala Lys Gly Ser Glu Gly Lys
50 55 60
gtc gaa gtg gta gaa gtt tct cct gat gaa aaa gtt ccc tat gca gca 240
Val Glu Val Val Glu Val Ser Pro Asp Glu Lys Val Pro Tyr Ala Ala
65 70 75 80
gtc gta cct cac caa gct gaa gtt atc ttc cgt caa cgt tat gaa atg 288
Val Val Pro His Gln Ala Glu Val Ile Phe Arg Gln Arg Tyr Glu Met
85 90 95
act gat aaa gaa ctt gag gat cgt att gaa tca tac tat gat ggg gtc 336
Thr Asp Lys Glu Leu Glu Asp Arg Ile Glu Ser Tyr Tyr Asp Gly Val
100 105 110
aat ctt ttc cgt tca atc aaa atc aaa ggt cat ttt aaa cac atg cac 384
Asn Leu Phe Arg Ser Ile Lys Ile Lys Gly His Phe Lys His Met His
115 120 125
gtg cgc atg att cca aaa tca aat gcg gac att aag ttt gct gat gtt 432
Val Arg Met Ile Pro Lys Ser Asn Ala Asp Ile Lys Phe Ala Asp Val
130 135 140
gcc act cgc caa cct gag tat gag gta gat gat att tca ggt aca atc 480
Ala Thr Arg Gln Pro Glu Tyr Glu Val Asp Asp Ile Ser Gly Thr Ile
145 150 155 160
gtt ggt atc tgg aca cct gaa atg ttc cac ggt gtg agt gtt gct ggt 528
Val Gly Ile Trp Thr Pro Glu Met Phe His Gly Val Ser Val Ala Gly
165 170 175
tac cat ctc cat ttt att tca gat gac ttg aca ttc ggt ggt cac gtt 576
Tyr His Leu His Phe Ile Ser Asp Asp Leu Thr Phe Gly Gly His Val
180 185 190
atg gat ttt gtt atc gag aat ggt att att gaa gtt gga cca gtt gac 624
Met Asp Phe Val Ile Glu Asn Gly Ile Ile Glu Val Gly Pro Val Asp
195 200 205
caa ctc gat caa cgc ttc cca gtt caa gac cgt caa tat ctc ttt gct 672
Gln Leu Asp Gln Arg Phe Pro Val Gln Asp Arg Gln Tyr Leu Phe Ala
210 215 220
aaa ttc aat gtt gat gaa atg cgt aaa gat att act aag gct gaa taa 720
Lys Phe Asn Val Asp Glu Met Arg Lys Asp Ile Thr Lys Ala Glu
225 230 235
<210> 92
<211> 239
<212> PRT
<213> 嗜热链球菌（Streptococcus thermophilus）
<400> 92
Met Ser Glu Ala Ile Lys Leu Phe Gln Tyr Asn Thr Leu Gly Ala Leu
1 5 10 15
Met Ala Gly Leu Tyr Gly Gly Thr Leu Thr Val Gly Glu Leu Leu Glu
20 25 30
His Gly Asp Leu Gly Leu Gly Thr Leu Asp Ser Ile Asp Gly Glu Leu
35 40 45
Ile Val Leu Asp Gly Lys Ala Tyr Gln Ala Lys Gly Ser Glu Gly Lys
50 55 60
Val Glu Val Val Glu Val Ser Pro Asp Glu Lys Val Pro Tyr Ala Ala
65 70 75 80
Val Val Pro His Gln Ala Glu Val Ile Phe Arg Gln Arg Tyr Glu Met
85 90 95
Thr Asp Lys Glu Leu Glu Asp Arg Ile Glu Ser Tyr Tyr Asp Gly Val
100 105 110
Asn Leu Phe Arg Ser Ile Lys Ile Lys Gly His Phe Lys His Met His
115 120 125
Val Arg Met Ile Pro Lys Ser Asn Ala Asp Ile Lys Phe Ala Asp Val
130 135 140
Ala Thr Arg Gln Pro Glu Tyr Glu Val Asp Asp Ile Ser Gly Thr Ile
145 150 155 160
Val Gly Ile Trp Thr Pro Glu Met Phe His Gly Val Ser Val Ala Gly
165 170 175
Tyr His Leu His Phe Ile Ser Asp Asp Leu Thr Phe Gly Gly His Val
180 185 190
Met Asp Phe Val Ile Glu Asn Gly Ile Ile Glu Val Gly Pro Val Asp
195 200 205
Gln Leu Asp Gln Arg Phe Pro Val Gln Asp Arg Gln Tyr Leu Phe Ala
210 215 220
Lys Phe Asn Val Asp Glu Met Arg Lys Asp Ile Thr Lys Ala Glu
225 230 235
<210> 93
<211> 717
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(717)
<223> 酒类酒球菌 PSU-1; 基因 OEOE_1704 编码a-乙酰乳酸脱羧酶
<400> 93
atg aaa gat tta aca aaa gct tat caa cat ggc act ttg gct caa att 48
Met Lys Asp Leu Thr Lys Ala Tyr Gln His Gly Thr Leu Ala Gln Ile
1 5 10 15
atg gat ggc caa tat gat ggg aca ata ctg ctt aaa gat ctt ctc gaa 96
Met Asp Gly Gln Tyr Asp Gly Thr Ile Leu Leu Lys Asp Leu Leu Glu
20 25 30
cac ggc gat ttc ggt att ggt aca aca acc gga atc ggg gtc gaa tta 144
His Gly Asp Phe Gly Ile Gly Thr Thr Thr Gly Ile Gly Val Glu Leu
35 40 45
ata gtt ttg gat ggg gtg gct tat gga atc ccc agc agc gga aaa gtc 192
Ile Val Leu Asp Gly Val Ala Tyr Gly Ile Pro Ser Ser Gly Lys Val
50 55 60
caa aaa atg gac atc gag cac gaa aaa gca ccc ttt gca aat att aac 240
Gln Lys Met Asp Ile Glu His Glu Lys Ala Pro Phe Ala Asn Ile Asn
65 70 75 80
tac ttc gat caa aag ttg aag agc gaa agc cta att aat ctt gat tcc 288
Tyr Phe Asp Gln Lys Leu Lys Ser Glu Ser Leu Ile Asn Leu Asp Ser
85 90 95
gat agt ttt caa aaa agg gtt gaa gaa gaa tat aaa ctt aaa aat gtc 336
Asp Ser Phe Gln Lys Arg Val Glu Glu Glu Tyr Lys Leu Lys Asn Val
100 105 110
ttt gcc gca att aga gta cac gga gaa ttt aca aat gtt ttg gcg cga 384
Phe Ala Ala Ile Arg Val His Gly Glu Phe Thr Asn Val Leu Ala Arg
115 120 125
tca gcc gat aaa caa gaa aaa cca tac ccg cca ttt tca aag gtc gcg 432
Ser Ala Asp Lys Gln Glu Lys Pro Tyr Pro Pro Phe Ser Lys Val Ala
130 135 140
gca gcg caa cat gaa ttc cat gct gat tca ctg acg gca acg atg gtt 480
Ala Ala Gln His Glu Phe His Ala Asp Ser Leu Thr Ala Thr Met Val
145 150 155 160
ggc tat tat tca gca gcg atg tat gaa ggg aca acc gcg gcc ggc ttt 528
Gly Tyr Tyr Ser Ala Ala Met Tyr Glu Gly Thr Thr Ala Ala Gly Phe
165 170 175
cac ctt cac att ctc tcc gat gat cgt caa ttc gga ggg cac cta tta 576
His Leu His Ile Leu Ser Asp Asp Arg Gln Phe Gly Gly His Leu Leu
180 185 190
gat ttt aaa atc aaa aaa gcc gac ctc cag gtt cag att ttt cag gat 624
Asp Phe Lys Ile Lys Lys Ala Asp Leu Gln Val Gln Ile Phe Gln Asp
195 200 205
ttc cag ttg cat cta cca att gaa aat ccc gat ttt cgc cga cgc gaa 672
Phe Gln Leu His Leu Pro Ile Glu Asn Pro Asp Phe Arg Arg Arg Glu
210 215 220
tta gac ttg gaa act tta aaa aaa gcg att gaa aag aca gaa taa 717
Leu Asp Leu Glu Thr Leu Lys Lys Ala Ile Glu Lys Thr Glu
225 230 235
<210> 94
<211> 238
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 94
Met Lys Asp Leu Thr Lys Ala Tyr Gln His Gly Thr Leu Ala Gln Ile
1 5 10 15
Met Asp Gly Gln Tyr Asp Gly Thr Ile Leu Leu Lys Asp Leu Leu Glu
20 25 30
His Gly Asp Phe Gly Ile Gly Thr Thr Thr Gly Ile Gly Val Glu Leu
35 40 45
Ile Val Leu Asp Gly Val Ala Tyr Gly Ile Pro Ser Ser Gly Lys Val
50 55 60
Gln Lys Met Asp Ile Glu His Glu Lys Ala Pro Phe Ala Asn Ile Asn
65 70 75 80
Tyr Phe Asp Gln Lys Leu Lys Ser Glu Ser Leu Ile Asn Leu Asp Ser
85 90 95
Asp Ser Phe Gln Lys Arg Val Glu Glu Glu Tyr Lys Leu Lys Asn Val
100 105 110
Phe Ala Ala Ile Arg Val His Gly Glu Phe Thr Asn Val Leu Ala Arg
115 120 125
Ser Ala Asp Lys Gln Glu Lys Pro Tyr Pro Pro Phe Ser Lys Val Ala
130 135 140
Ala Ala Gln His Glu Phe His Ala Asp Ser Leu Thr Ala Thr Met Val
145 150 155 160
Gly Tyr Tyr Ser Ala Ala Met Tyr Glu Gly Thr Thr Ala Ala Gly Phe
165 170 175
His Leu His Ile Leu Ser Asp Asp Arg Gln Phe Gly Gly His Leu Leu
180 185 190
Asp Phe Lys Ile Lys Lys Ala Asp Leu Gln Val Gln Ile Phe Gln Asp
195 200 205
Phe Gln Leu His Leu Pro Ile Glu Asn Pro Asp Phe Arg Arg Arg Glu
210 215 220
Leu Asp Leu Glu Thr Leu Lys Lys Ala Ile Glu Lys Thr Glu
225 230 235
<210> 95
<211> 750
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(750)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_2135 编码a-乙酰乳酸脱羧酶
<400> 95
atg cgt act gct gca gaa aat caa aat aca gag caa att tta aca agc 48
Met Arg Thr Ala Ala Glu Asn Gln Asn Thr Glu Gln Ile Leu Thr Ser
1 5 10 15
cgg acc gat gaa gta tac caa ttg tcc acg atg aca tcg ctt ctt gac 96
Arg Thr Asp Glu Val Tyr Gln Leu Ser Thr Met Thr Ser Leu Leu Asp
20 25 30
ggc gta tac gaa agc gac aag acg ttt gct gaa tta aaa aag ttc ggc 144
Gly Val Tyr Glu Ser Asp Lys Thr Phe Ala Glu Leu Lys Lys Phe Gly
35 40 45
gat ttc ggc atc ggc act ttc aac cat ttg gac ggg gaa ttg att gcc 192
Asp Phe Gly Ile Gly Thr Phe Asn His Leu Asp Gly Glu Leu Ile Ala
50 55 60
ttt gac aat gcg ttc tat cag ctg aag gac ggg acg gca aaa cgt gta 240
Phe Asp Asn Ala Phe Tyr Gln Leu Lys Asp Gly Thr Ala Lys Arg Val
65 70 75 80
cag cct gaa gac aag tca ccg ttt tgc tcg ctt gca cat ttt tcg gag 288
Gln Pro Glu Asp Lys Ser Pro Phe Cys Ser Leu Ala His Phe Ser Glu
85 90 95
gat atc acc tat aca gct gaa ggt cct cta gca aaa ccc gaa ctg gaa 336
Asp Ile Thr Tyr Thr Ala Glu Gly Pro Leu Ala Lys Pro Glu Leu Glu
100 105 110
gac ttg atc aaa gat ctc gtc cgc agc gaa aat tta ttt tat gcc att 384
Asp Leu Ile Lys Asp Leu Val Arg Ser Glu Asn Leu Phe Tyr Ala Ile
115 120 125
cgt gtg gac ggt gtt ttt aaa aaa atg aac aca aga acc gtc tcc tac 432
Arg Val Asp Gly Val Phe Lys Lys Met Asn Thr Arg Thr Val Ser Tyr
130 135 140
cag gaa aaa ccg gtt ccc atg acg gaa gcg gtg aaa tcc cag ccg gtt 480
Gln Glu Lys Pro Val Pro Met Thr Glu Ala Val Lys Ser Gln Pro Val
145 150 155 160
tac tcg ttt gaa aat aca aaa gga acg ctt tct ggt ttt tgg acg ccg 528
Tyr Ser Phe Glu Asn Thr Lys Gly Thr Leu Ser Gly Phe Trp Thr Pro
165 170 175
atg ttt gca caa gga atc gcg gtc gcc ggc ttc cac ctc cat ttt att 576
Met Phe Ala Gln Gly Ile Ala Val Ala Gly Phe His Leu His Phe Ile
180 185 190
gac gac aaa cgc acc ggc ggc ggc cat gtc ttt gac tat gtg ctt gac 624
Asp Asp Lys Arg Thr Gly Gly Gly His Val Phe Asp Tyr Val Leu Asp
195 200 205
tac ggg acg atc cgg atc agc aaa aaa acg cat atg cat ctg gaa ctt 672
Tyr Gly Thr Ile Arg Ile Ser Lys Lys Thr His Met His Leu Glu Leu
210 215 220
ccg gaa aca gac gct ttt tta aat gcg aac ctt tcc cgc gcc aat ttg 720
Pro Glu Thr Asp Ala Phe Leu Asn Ala Asn Leu Ser Arg Ala Asn Leu
225 230 235 240
gca gag gaa ctg gaa aag aca gaa ggc tga 750
Ala Glu Glu Leu Glu Lys Thr Glu Gly
245
<210> 96
<211> 249
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 96
Met Arg Thr Ala Ala Glu Asn Gln Asn Thr Glu Gln Ile Leu Thr Ser
1 5 10 15
Arg Thr Asp Glu Val Tyr Gln Leu Ser Thr Met Thr Ser Leu Leu Asp
20 25 30
Gly Val Tyr Glu Ser Asp Lys Thr Phe Ala Glu Leu Lys Lys Phe Gly
35 40 45
Asp Phe Gly Ile Gly Thr Phe Asn His Leu Asp Gly Glu Leu Ile Ala
50 55 60
Phe Asp Asn Ala Phe Tyr Gln Leu Lys Asp Gly Thr Ala Lys Arg Val
65 70 75 80
Gln Pro Glu Asp Lys Ser Pro Phe Cys Ser Leu Ala His Phe Ser Glu
85 90 95
Asp Ile Thr Tyr Thr Ala Glu Gly Pro Leu Ala Lys Pro Glu Leu Glu
100 105 110
Asp Leu Ile Lys Asp Leu Val Arg Ser Glu Asn Leu Phe Tyr Ala Ile
115 120 125
Arg Val Asp Gly Val Phe Lys Lys Met Asn Thr Arg Thr Val Ser Tyr
130 135 140
Gln Glu Lys Pro Val Pro Met Thr Glu Ala Val Lys Ser Gln Pro Val
145 150 155 160
Tyr Ser Phe Glu Asn Thr Lys Gly Thr Leu Ser Gly Phe Trp Thr Pro
165 170 175
Met Phe Ala Gln Gly Ile Ala Val Ala Gly Phe His Leu His Phe Ile
180 185 190
Asp Asp Lys Arg Thr Gly Gly Gly His Val Phe Asp Tyr Val Leu Asp
195 200 205
Tyr Gly Thr Ile Arg Ile Ser Lys Lys Thr His Met His Leu Glu Leu
210 215 220
Pro Glu Thr Asp Ala Phe Leu Asn Ala Asn Leu Ser Arg Ala Asn Leu
225 230 235 240
Ala Glu Glu Leu Glu Lys Thr Glu Gly
245
<210> 97
<211> 771
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(771)
<223> 乳酸乳球菌乳脂亚种MG1363; 基因 llmg_0339
编码双乙酰还原酶
<400> 97
atg ggg aaa tta gcg att gta acg gga agc gga caa ggt att ggg gaa 48
Met Gly Lys Leu Ala Ile Val Thr Gly Ser Gly Gln Gly Ile Gly Glu
1 5 10 15
ggt att gtc tat cgg tta gca aaa gat gga ttt gcg gtt gcc gta gca 96
Gly Ile Val Tyr Arg Leu Ala Lys Asp Gly Phe Ala Val Ala Val Ala
20 25 30
gat att aat cct caa aca gca aaa aat gtt gca aaa act ttg ata gat 144
Asp Ile Asn Pro Gln Thr Ala Lys Asn Val Ala Lys Thr Leu Ile Asp
35 40 45
aaa gga ttt gaa gct aaa gct tac gtg ctt gat gtt gcg aac cgt tcg 192
Lys Gly Phe Glu Ala Lys Ala Tyr Val Leu Asp Val Ala Asn Arg Ser
50 55 60
gca gtt ttt cag ctt att gaa caa gcg gtg agt gat ttt ggt gaa ctt 240
Ala Val Phe Gln Leu Ile Glu Gln Ala Val Ser Asp Phe Gly Glu Leu
65 70 75 80
gct gta ttt gtt aat aat gct ggg gtt gct ttt att gat agc att ata 288
Ala Val Phe Val Asn Asn Ala Gly Val Ala Phe Ile Asp Ser Ile Ile
85 90 95
gat tct gat tca gaa agg gtt gaa cga cta ttt gat gtc aat atc aaa 336
Asp Ser Asp Ser Glu Arg Val Glu Arg Leu Phe Asp Val Asn Ile Lys
100 105 110
ggt act tat tgg gga ata caa gca gca gct acc caa ttt aaa aaa caa 384
Gly Thr Tyr Trp Gly Ile Gln Ala Ala Ala Thr Gln Phe Lys Lys Gln
115 120 125
ggt cat ggc gga cga att atc aat gcg gct tcg ctt gca agt gtt gaa 432
Gly His Gly Gly Arg Ile Ile Asn Ala Ala Ser Leu Ala Ser Val Glu
130 135 140
ggt tct gcc ttg caa agt gct tat tct gct tca aaa ttt gca ata cgt 480
Gly Ser Ala Leu Gln Ser Ala Tyr Ser Ala Ser Lys Phe Ala Ile Arg
145 150 155 160
gga ttg aca cag tcg gca gcg aaa gaa ctt gca aaa gac tat atc aca 528
Gly Leu Thr Gln Ser Ala Ala Lys Glu Leu Ala Lys Asp Tyr Ile Thr
165 170 175
gtg aat gct tat aat cct gga att gta cgc acc gct atg cga gat gag 576
Val Asn Ala Tyr Asn Pro Gly Ile Val Arg Thr Ala Met Arg Asp Glu
180 185 190
att gat aaa aaa acg gct caa atc aaa gga att agc att gaa cag caa 624
Ile Asp Lys Lys Thr Ala Gln Ile Lys Gly Ile Ser Ile Glu Gln Gln
195 200 205
caa cag aat tgt ttg aat gaa atc tac ctc gga cgt gaa gga aga cca 672
Gln Gln Asn Cys Leu Asn Glu Ile Tyr Leu Gly Arg Glu Gly Arg Pro
210 215 220
gaa gat gtt gca gaa gtt gtg gcc tgg ttt gct tca gat gca gca gaa 720
Glu Asp Val Ala Glu Val Val Ala Trp Phe Ala Ser Asp Ala Ala Glu
225 230 235 240
tat att aca gga caa tct atc tta gtg gat ggc ggg atg aga ttc aat 768
Tyr Ile Thr Gly Gln Ser Ile Leu Val Asp Gly Gly Met Arg Phe Asn
245 250 255
tag 771
<210> 98
<211> 256
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 98
Met Gly Lys Leu Ala Ile Val Thr Gly Ser Gly Gln Gly Ile Gly Glu
1 5 10 15
Gly Ile Val Tyr Arg Leu Ala Lys Asp Gly Phe Ala Val Ala Val Ala
20 25 30
Asp Ile Asn Pro Gln Thr Ala Lys Asn Val Ala Lys Thr Leu Ile Asp
35 40 45
Lys Gly Phe Glu Ala Lys Ala Tyr Val Leu Asp Val Ala Asn Arg Ser
50 55 60
Ala Val Phe Gln Leu Ile Glu Gln Ala Val Ser Asp Phe Gly Glu Leu
65 70 75 80
Ala Val Phe Val Asn Asn Ala Gly Val Ala Phe Ile Asp Ser Ile Ile
85 90 95
Asp Ser Asp Ser Glu Arg Val Glu Arg Leu Phe Asp Val Asn Ile Lys
100 105 110
Gly Thr Tyr Trp Gly Ile Gln Ala Ala Ala Thr Gln Phe Lys Lys Gln
115 120 125
Gly His Gly Gly Arg Ile Ile Asn Ala Ala Ser Leu Ala Ser Val Glu
130 135 140
Gly Ser Ala Leu Gln Ser Ala Tyr Ser Ala Ser Lys Phe Ala Ile Arg
145 150 155 160
Gly Leu Thr Gln Ser Ala Ala Lys Glu Leu Ala Lys Asp Tyr Ile Thr
165 170 175
Val Asn Ala Tyr Asn Pro Gly Ile Val Arg Thr Ala Met Arg Asp Glu
180 185 190
Ile Asp Lys Lys Thr Ala Gln Ile Lys Gly Ile Ser Ile Glu Gln Gln
195 200 205
Gln Gln Asn Cys Leu Asn Glu Ile Tyr Leu Gly Arg Glu Gly Arg Pro
210 215 220
Glu Asp Val Ala Glu Val Val Ala Trp Phe Ala Ser Asp Ala Ala Glu
225 230 235 240
Tyr Ile Thr Gly Gln Ser Ile Leu Val Asp Gly Gly Met Arg Phe Asn
245 250 255
<210> 99
<211> 762
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(762)
<223> 乳酸乳球菌乳脂亚种 MG1363; 基因 llmg_1641
编码双乙酰还原酶
<400> 99
atg tct aaa gtt gca gca gtt act ggc gca ggt caa gga att ggc ttt 48
Met Ser Lys Val Ala Ala Val Thr Gly Ala Gly Gln Gly Ile Gly Phe
1 5 10 15
gct atc gca aaa cgt tta tat aat gat ggg ttt aaa gtc gca atc att 96
Ala Ile Ala Lys Arg Leu Tyr Asn Asp Gly Phe Lys Val Ala Ile Ile
20 25 30
gat tac aat gaa gaa aca gct caa caa gca gct aag gaa ctt ggt ggc 144
Asp Tyr Asn Glu Glu Thr Ala Gln Gln Ala Ala Lys Glu Leu Gly Gly
35 40 45
gaa tca ttt gct ctt aaa gca gat gtt tct gac cgt gac caa gta gtt 192
Glu Ser Phe Ala Leu Lys Ala Asp Val Ser Asp Arg Asp Gln Val Val
50 55 60
gcc gct tta gaa gct gtt gtt gaa aaa ttt ggt gat ttg aat gta gta 240
Ala Ala Leu Glu Ala Val Val Glu Lys Phe Gly Asp Leu Asn Val Val
65 70 75 80
gta aat aat gca gga atc gcc cca acc act ccg att gaa aca att act 288
Val Asn Asn Ala Gly Ile Ala Pro Thr Thr Pro Ile Glu Thr Ile Thr
85 90 95
cct gaa caa ttt cat caa gtt tat aat att aat gtt ggt gga gtt ttg 336
Pro Glu Gln Phe His Gln Val Tyr Asn Ile Asn Val Gly Gly Val Leu
100 105 110
tgg gga aca cag gca gcc aca gca ctt ttc cgt aaa cta ggt cat ggt 384
Trp Gly Thr Gln Ala Ala Thr Ala Leu Phe Arg Lys Leu Gly His Gly
115 120 125
ggt aag att att aat gca act tcg caa gca ggt gtc gta ggg aat cct 432
Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala Gly Val Val Gly Asn Pro
130 135 140
aac cta atg ctt tat tct tct tca aaa ttc gct gtt cgt gga atg aca 480
Asn Leu Met Leu Tyr Ser Ser Ser Lys Phe Ala Val Arg Gly Met Thr
145 150 155 160
caa att gcc gct cgc gat cta gca gaa gaa gga att aca gtt aat gcc 528
Gln Ile Ala Ala Arg Asp Leu Ala Glu Glu Gly Ile Thr Val Asn Ala
165 170 175
tat gca cca ggg att gtt aaa aca cca atg atg ttt gat att gca cat 576
Tyr Ala Pro Gly Ile Val Lys Thr Pro Met Met Phe Asp Ile Ala His
180 185 190
caa gtg ggt aaa aat gca ggt aaa gat gac gaa tgg ggc atg cag act 624
Gln Val Gly Lys Asn Ala Gly Lys Asp Asp Glu Trp Gly Met Gln Thr
195 200 205
ttt gcc aaa gat atc gcg atg aaa cgt ttg tca gag cct gag gac gtg 672
Phe Ala Lys Asp Ile Ala Met Lys Arg Leu Ser Glu Pro Glu Asp Val
210 215 220
gcc aat gtg gtt tct ttc ctt gct ggt cct gat tct aat tat att acg 720
Ala Asn Val Val Ser Phe Leu Ala Gly Pro Asp Ser Asn Tyr Ile Thr
225 230 235 240
ggt caa aca att att gtt gat ggt gga atg caa ttt cat taa 762
Gly Gln Thr Ile Ile Val Asp Gly Gly Met Gln Phe His
245 250
<210> 100
<211> 253
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 100
Met Ser Lys Val Ala Ala Val Thr Gly Ala Gly Gln Gly Ile Gly Phe
1 5 10 15
Ala Ile Ala Lys Arg Leu Tyr Asn Asp Gly Phe Lys Val Ala Ile Ile
20 25 30
Asp Tyr Asn Glu Glu Thr Ala Gln Gln Ala Ala Lys Glu Leu Gly Gly
35 40 45
Glu Ser Phe Ala Leu Lys Ala Asp Val Ser Asp Arg Asp Gln Val Val
50 55 60
Ala Ala Leu Glu Ala Val Val Glu Lys Phe Gly Asp Leu Asn Val Val
65 70 75 80
Val Asn Asn Ala Gly Ile Ala Pro Thr Thr Pro Ile Glu Thr Ile Thr
85 90 95
Pro Glu Gln Phe His Gln Val Tyr Asn Ile Asn Val Gly Gly Val Leu
100 105 110
Trp Gly Thr Gln Ala Ala Thr Ala Leu Phe Arg Lys Leu Gly His Gly
115 120 125
Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala Gly Val Val Gly Asn Pro
130 135 140
Asn Leu Met Leu Tyr Ser Ser Ser Lys Phe Ala Val Arg Gly Met Thr
145 150 155 160
Gln Ile Ala Ala Arg Asp Leu Ala Glu Glu Gly Ile Thr Val Asn Ala
165 170 175
Tyr Ala Pro Gly Ile Val Lys Thr Pro Met Met Phe Asp Ile Ala His
180 185 190
Gln Val Gly Lys Asn Ala Gly Lys Asp Asp Glu Trp Gly Met Gln Thr
195 200 205
Phe Ala Lys Asp Ile Ala Met Lys Arg Leu Ser Glu Pro Glu Asp Val
210 215 220
Ala Asn Val Val Ser Phe Leu Ala Gly Pro Asp Ser Asn Tyr Ile Thr
225 230 235 240
Gly Gln Thr Ile Ile Val Asp Gly Gly Met Gln Phe His
245 250
<210> 101
<211> 780
<212> DNA
<213> 戊糖片球菌（Pediococcus pentosaceus）
<220>
<221> CDS
<222> (1)..(780)
<223> 戊糖片球菌 ATCC 25745; 基因 PEPE_0286 编码乙偶姻还原酶
<400> 101
atg gca aac aaa acc aaa gtt gca tta gta acg gga gca gga caa gga 48
Met Ala Asn Lys Thr Lys Val Ala Leu Val Thr Gly Ala Gly Gln Gly
1 5 10 15
att ggg gca gca att gcg acc cga ttg gct aac gat gga ttt gcg gtg 96
Ile Gly Ala Ala Ile Ala Thr Arg Leu Ala Asn Asp Gly Phe Ala Val
20 25 30
gca gtg gca gat atg aat act gaa act gct aac aaa gtt gct gaa aaa 144
Ala Val Ala Asp Met Asn Thr Glu Thr Ala Asn Lys Val Ala Glu Lys
35 40 45
att aat agc aat ggt gga agg gct ctt cca atc gtg gtt aat gta gca 192
Ile Asn Ser Asn Gly Gly Arg Ala Leu Pro Ile Val Val Asn Val Ala
50 55 60
gaa cgt gat aat gtg ttt gaa gcc gtt agg act acg att cag gaa cta 240
Glu Arg Asp Asn Val Phe Glu Ala Val Arg Thr Thr Ile Gln Glu Leu
65 70 75 80
ggt gga ctt gat gtg ttg gtc aat aat gcc ggc cta gga cct acc aca 288
Gly Gly Leu Asp Val Leu Val Asn Asn Ala Gly Leu Gly Pro Thr Thr
85 90 95
cca att gat aca att aca cca gaa caa ttt gat aaa gtt tat cac gtt 336
Pro Ile Asp Thr Ile Thr Pro Glu Gln Phe Asp Lys Val Tyr His Val
100 105 110
aat gta ggt gga gta ctt tgg gga att caa gca gca acg gaa gca ttt 384
Asn Val Gly Gly Val Leu Trp Gly Ile Gln Ala Ala Thr Glu Ala Phe
115 120 125
aag aaa tta gga cat ggt gga aaa atc atc aat gcg act tct caa gcc 432
Lys Lys Leu Gly His Gly Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala
130 135 140
gga gtt gtt gga aac cct aat ttg gcc ctt tac tca ggg act aag ttt 480
Gly Val Val Gly Asn Pro Asn Leu Ala Leu Tyr Ser Gly Thr Lys Phe
145 150 155 160
gct gtc cga gga att act caa gta gca gca cgt gat ttg gcg gat ttt 528
Ala Val Arg Gly Ile Thr Gln Val Ala Ala Arg Asp Leu Ala Asp Phe
165 170 175
ggt att aca gtt aat gct tat gca ccg gga att gta cga act ccg atg 576
Gly Ile Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Arg Thr Pro Met
180 185 190
atg atg gat atc gca cat gaa gtt gga caa aat gct ggt aaa gat gac 624
Met Met Asp Ile Ala His Glu Val Gly Gln Asn Ala Gly Lys Asp Asp
195 200 205
gaa tgg ggt atg caa cag ttt gct aaa gac att acc tta aaa cgt ttg 672
Glu Trp Gly Met Gln Gln Phe Ala Lys Asp Ile Thr Leu Lys Arg Leu
210 215 220
tca gaa cca gaa gat gtg gca gct gca gta tca ttc tta gca gga cca 720
Ser Glu Pro Glu Asp Val Ala Ala Ala Val Ser Phe Leu Ala Gly Pro
225 230 235 240
gat tca aat tat gta act gga caa aca att att gta gat ggt gga atg 768
Asp Ser Asn Tyr Val Thr Gly Gln Thr Ile Ile Val Asp Gly Gly Met
245 250 255
caa ttt cat taa 780
Gln Phe His
<210> 102
<211> 259
<212> PRT
<213> 戊糖片球菌（Pediococcus pentosaceus）
<400> 102
Met Ala Asn Lys Thr Lys Val Ala Leu Val Thr Gly Ala Gly Gln Gly
1 5 10 15
Ile Gly Ala Ala Ile Ala Thr Arg Leu Ala Asn Asp Gly Phe Ala Val
20 25 30
Ala Val Ala Asp Met Asn Thr Glu Thr Ala Asn Lys Val Ala Glu Lys
35 40 45
Ile Asn Ser Asn Gly Gly Arg Ala Leu Pro Ile Val Val Asn Val Ala
50 55 60
Glu Arg Asp Asn Val Phe Glu Ala Val Arg Thr Thr Ile Gln Glu Leu
65 70 75 80
Gly Gly Leu Asp Val Leu Val Asn Asn Ala Gly Leu Gly Pro Thr Thr
85 90 95
Pro Ile Asp Thr Ile Thr Pro Glu Gln Phe Asp Lys Val Tyr His Val
100 105 110
Asn Val Gly Gly Val Leu Trp Gly Ile Gln Ala Ala Thr Glu Ala Phe
115 120 125
Lys Lys Leu Gly His Gly Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala
130 135 140
Gly Val Val Gly Asn Pro Asn Leu Ala Leu Tyr Ser Gly Thr Lys Phe
145 150 155 160
Ala Val Arg Gly Ile Thr Gln Val Ala Ala Arg Asp Leu Ala Asp Phe
165 170 175
Gly Ile Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Arg Thr Pro Met
180 185 190
Met Met Asp Ile Ala His Glu Val Gly Gln Asn Ala Gly Lys Asp Asp
195 200 205
Glu Trp Gly Met Gln Gln Phe Ala Lys Asp Ile Thr Leu Lys Arg Leu
210 215 220
Ser Glu Pro Glu Asp Val Ala Ala Ala Val Ser Phe Leu Ala Gly Pro
225 230 235 240
Asp Ser Asn Tyr Val Thr Gly Gln Thr Ile Ile Val Asp Gly Gly Met
245 250 255
Gln Phe His
<210> 103
<211> 777
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(777)
<223> 肠膜明串珠菌肠膜亚种 ATCC 8293; 基因
LEUM_0117 编码乙偶姻还原酶
<400> 103
atg tca aag aaa gta gca att att aca ggc agt ggt aga gga ata gga 48
Met Ser Lys Lys Val Ala Ile Ile Thr Gly Ser Gly Arg Gly Ile Gly
1 5 10 15
aaa gca att gct gag aga ctc gcg aca gaa aat tat tac gtt gca gta 96
Lys Ala Ile Ala Glu Arg Leu Ala Thr Glu Asn Tyr Tyr Val Ala Val
20 25 30
gtc gac att gat gaa tct agt gct aaa tat gtg agt gat agt atc aac 144
Val Asp Ile Asp Glu Ser Ser Ala Lys Tyr Val Ser Asp Ser Ile Asn
35 40 45
gct att aag gaa aac caa gcc aaa tat tat gtt tta gat gtt gct gat 192
Ala Ile Lys Glu Asn Gln Ala Lys Tyr Tyr Val Leu Asp Val Ala Asp
50 55 60
cga aaa tca gtt ttt gat ttg gtt gat aaa gta gta gct gat ttt ggg 240
Arg Lys Ser Val Phe Asp Leu Val Asp Lys Val Val Ala Asp Phe Gly
65 70 75 80
cgt cta gat gta ttt gtt aat aac gca ggg att gcc tac att gat agc 288
Arg Leu Asp Val Phe Val Asn Asn Ala Gly Ile Ala Tyr Ile Asp Ser
85 90 95
att atc gat agt gat ccg gaa aaa gtt gag cgg ttg ttt gat gtt aac 336
Ile Ile Asp Ser Asp Pro Glu Lys Val Glu Arg Leu Phe Asp Val Asn
100 105 110
ctt aag ggc act ttt tgg gga atc cag gcg gct gca aaa cag ttg aaa 384
Leu Lys Gly Thr Phe Trp Gly Ile Gln Ala Ala Ala Lys Gln Leu Lys
115 120 125
aag caa ggg agt ggc gga agg ata atc aat gct gct tca ctg gct gca 432
Lys Gln Gly Ser Gly Gly Arg Ile Ile Asn Ala Ala Ser Leu Ala Ala
130 135 140
gtg gaa gga tct gca cta caa ggc gca tat tca gct tcc aaa ttt gcc 480
Val Glu Gly Ser Ala Leu Gln Gly Ala Tyr Ser Ala Ser Lys Phe Ala
145 150 155 160
att aga gga tta ggt caa tct gcg gca aaa gaa ctc gca aaa tat aat 528
Ile Arg Gly Leu Gly Gln Ser Ala Ala Lys Glu Leu Ala Lys Tyr Asn
165 170 175
atc act gta aat act tat gat ccg ggt att gtt att aca cct ttg cgc 576
Ile Thr Val Asn Thr Tyr Asp Pro Gly Ile Val Ile Thr Pro Leu Arg
180 185 190
gat tat att gat cag cga aca gca caa att aaa aac aca aca gct gag 624
Asp Tyr Ile Asp Gln Arg Thr Ala Gln Ile Lys Asn Thr Thr Ala Glu
195 200 205
att caa agg caa agt gtt gtc aat gaa att gcg tta gga aga gcg gct 672
Ile Gln Arg Gln Ser Val Val Asn Glu Ile Ala Leu Gly Arg Ala Ala
210 215 220
ttg cct gat gat gta gcc gat gtt gta tca ttt ttg gtt tca cag cag 720
Leu Pro Asp Asp Val Ala Asp Val Val Ser Phe Leu Val Ser Gln Gln
225 230 235 240
gca aag tat att act ggg caa tcg att tta att gat ggt ggt atg aga 768
Ala Lys Tyr Ile Thr Gly Gln Ser Ile Leu Ile Asp Gly Gly Met Arg
245 250 255
ttt cat tga 777
Phe His
<210> 104
<211> 258
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 104
Met Ser Lys Lys Val Ala Ile Ile Thr Gly Ser Gly Arg Gly Ile Gly
1 5 10 15
Lys Ala Ile Ala Glu Arg Leu Ala Thr Glu Asn Tyr Tyr Val Ala Val
20 25 30
Val Asp Ile Asp Glu Ser Ser Ala Lys Tyr Val Ser Asp Ser Ile Asn
35 40 45
Ala Ile Lys Glu Asn Gln Ala Lys Tyr Tyr Val Leu Asp Val Ala Asp
50 55 60
Arg Lys Ser Val Phe Asp Leu Val Asp Lys Val Val Ala Asp Phe Gly
65 70 75 80
Arg Leu Asp Val Phe Val Asn Asn Ala Gly Ile Ala Tyr Ile Asp Ser
85 90 95
Ile Ile Asp Ser Asp Pro Glu Lys Val Glu Arg Leu Phe Asp Val Asn
100 105 110
Leu Lys Gly Thr Phe Trp Gly Ile Gln Ala Ala Ala Lys Gln Leu Lys
115 120 125
Lys Gln Gly Ser Gly Gly Arg Ile Ile Asn Ala Ala Ser Leu Ala Ala
130 135 140
Val Glu Gly Ser Ala Leu Gln Gly Ala Tyr Ser Ala Ser Lys Phe Ala
145 150 155 160
Ile Arg Gly Leu Gly Gln Ser Ala Ala Lys Glu Leu Ala Lys Tyr Asn
165 170 175
Ile Thr Val Asn Thr Tyr Asp Pro Gly Ile Val Ile Thr Pro Leu Arg
180 185 190
Asp Tyr Ile Asp Gln Arg Thr Ala Gln Ile Lys Asn Thr Thr Ala Glu
195 200 205
Ile Gln Arg Gln Ser Val Val Asn Glu Ile Ala Leu Gly Arg Ala Ala
210 215 220
Leu Pro Asp Asp Val Ala Asp Val Val Ser Phe Leu Val Ser Gln Gln
225 230 235 240
Ala Lys Tyr Ile Thr Gly Gln Ser Ile Leu Ile Asp Gly Gly Met Arg
245 250 255
Phe His
<210> 105
<211> 777
<212> DNA
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<220>
<221> CDS
<222> (1)..(777)
<223> 肠膜明串珠菌肠膜亚种 ATCC 8293; 基因
LEUM_0145 编码乙偶姻还原酶
<400> 105
atg tca gaa aat aaa gtc gct ttg gta act gga gct ggt caa gga att 48
Met Ser Glu Asn Lys Val Ala Leu Val Thr Gly Ala Gly Gln Gly Ile
1 5 10 15
gga cag gca atc gca gaa cga ttg tca aaa gat ggg ttt aag gtt gcc 96
Gly Gln Ala Ile Ala Glu Arg Leu Ser Lys Asp Gly Phe Lys Val Ala
20 25 30
tta gtt gga cgt cat att gaa aaa gta caa aaa gta gct gat gaa att 144
Leu Val Gly Arg His Ile Glu Lys Val Gln Lys Val Ala Asp Glu Ile
35 40 45
aat gaa aat ggt ggt gaa gcc att gct att aag gct gat gtg gct aaa 192
Asn Glu Asn Gly Gly Glu Ala Ile Ala Ile Lys Ala Asp Val Ala Lys
50 55 60
cgt gat gaa gtt ttt gca gcc gtc aaa gaa acg aaa gaa aag ttc aat 240
Arg Asp Glu Val Phe Ala Ala Val Lys Glu Thr Lys Glu Lys Phe Asn
65 70 75 80
ggt ttt gat gtg att gtt aac aat gcg ggt gtt gcg cct acg aca cca 288
Gly Phe Asp Val Ile Val Asn Asn Ala Gly Val Ala Pro Thr Thr Pro
85 90 95
atc atg tca gta acg gaa gat gat atg aac tgg aca tgg ggc att aat 336
Ile Met Ser Val Thr Glu Asp Asp Met Asn Trp Thr Trp Gly Ile Asn
100 105 110
gtt aat ggt att gtc tgg gga aca caa gct gcg aca gaa gcc ttc aag 384
Val Asn Gly Ile Val Trp Gly Thr Gln Ala Ala Thr Glu Ala Phe Lys
115 120 125
gaa ttt ggt cat ggt ggc aaa atc att aat gca aca tcg caa gct ggt 432
Glu Phe Gly His Gly Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala Gly
130 135 140
gtg caa gga aat gct aat ttg act gcc tat gga tca aca aaa ttt gcc 480
Val Gln Gly Asn Ala Asn Leu Thr Ala Tyr Gly Ser Thr Lys Phe Ala
145 150 155 160
att cgc ggt att aca caa aca aca gct aaa gag tta gca gaa ttt ggt 528
Ile Arg Gly Ile Thr Gln Thr Thr Ala Lys Glu Leu Ala Glu Phe Gly
165 170 175
att aca gta aat gca ttt gcg cct gga att gtg aaa aca ccg atg atg 576
Ile Thr Val Asn Ala Phe Ala Pro Gly Ile Val Lys Thr Pro Met Met
180 185 190
gaa gat att gct cat gaa gta agc gtg aac gct ggt aaa gac gac gaa 624
Glu Asp Ile Ala His Glu Val Ser Val Asn Ala Gly Lys Asp Asp Glu
195 200 205
tgg gga atg gca caa ttt tca gaa gga atc acc tta gga cga cta agt 672
Trp Gly Met Ala Gln Phe Ser Glu Gly Ile Thr Leu Gly Arg Leu Ser
210 215 220
gaa ccg gaa gat ata gca aat gtg gtt tcc ttc ttg tct agc tca gat 720
Glu Pro Glu Asp Ile Ala Asn Val Val Ser Phe Leu Ser Ser Ser Asp
225 230 235 240
tca aac tat gtg aca ggc caa aca tta att gtt gat ggt ggt atg gta 768
Ser Asn Tyr Val Thr Gly Gln Thr Leu Ile Val Asp Gly Gly Met Val
245 250 255
ttt agt taa 777
Phe Ser
<210> 106
<211> 258
<212> PRT
<213> 肠膜明串珠菌（Leuconostoc mesenteroides）
<400> 106
Met Ser Glu Asn Lys Val Ala Leu Val Thr Gly Ala Gly Gln Gly Ile
1 5 10 15
Gly Gln Ala Ile Ala Glu Arg Leu Ser Lys Asp Gly Phe Lys Val Ala
20 25 30
Leu Val Gly Arg His Ile Glu Lys Val Gln Lys Val Ala Asp Glu Ile
35 40 45
Asn Glu Asn Gly Gly Glu Ala Ile Ala Ile Lys Ala Asp Val Ala Lys
50 55 60
Arg Asp Glu Val Phe Ala Ala Val Lys Glu Thr Lys Glu Lys Phe Asn
65 70 75 80
Gly Phe Asp Val Ile Val Asn Asn Ala Gly Val Ala Pro Thr Thr Pro
85 90 95
Ile Met Ser Val Thr Glu Asp Asp Met Asn Trp Thr Trp Gly Ile Asn
100 105 110
Val Asn Gly Ile Val Trp Gly Thr Gln Ala Ala Thr Glu Ala Phe Lys
115 120 125
Glu Phe Gly His Gly Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala Gly
130 135 140
Val Gln Gly Asn Ala Asn Leu Thr Ala Tyr Gly Ser Thr Lys Phe Ala
145 150 155 160
Ile Arg Gly Ile Thr Gln Thr Thr Ala Lys Glu Leu Ala Glu Phe Gly
165 170 175
Ile Thr Val Asn Ala Phe Ala Pro Gly Ile Val Lys Thr Pro Met Met
180 185 190
Glu Asp Ile Ala His Glu Val Ser Val Asn Ala Gly Lys Asp Asp Glu
195 200 205
Trp Gly Met Ala Gln Phe Ser Glu Gly Ile Thr Leu Gly Arg Leu Ser
210 215 220
Glu Pro Glu Asp Ile Ala Asn Val Val Ser Phe Leu Ser Ser Ser Asp
225 230 235 240
Ser Asn Tyr Val Thr Gly Gln Thr Leu Ile Val Asp Gly Gly Met Val
245 250 255
Phe Ser
<210> 107
<211> 780
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(780)
<223> 酒类酒球菌 PSU-1; 基因 OEOE_0693 编码乙偶姻还原酶
<400> 107
atg tca aat aaa gtt gca att att acg gga gct ggt caa gga att ggt 48
Met Ser Asn Lys Val Ala Ile Ile Thr Gly Ala Gly Gln Gly Ile Gly
1 5 10 15
aaa gca att gct caa aga ctg gca aaa gac ggt ttc aag acg gga ctg 96
Lys Ala Ile Ala Gln Arg Leu Ala Lys Asp Gly Phe Lys Thr Gly Leu
20 25 30
att ggt aga cat tta gaa aaa gtc gaa gca gtg gca aaa gaa cta aat 144
Ile Gly Arg His Leu Glu Lys Val Glu Ala Val Ala Lys Glu Leu Asn
35 40 45
gaa gaa tat ggt tcc gat act gct ata gcc aaa aaa gcc gat gtc tca 192
Glu Glu Tyr Gly Ser Asp Thr Ala Ile Ala Lys Lys Ala Asp Val Ser
50 55 60
aaa cgc gac gaa gtt ttt gca gct ttt caa gaa ata agc gac aat ttc 240
Lys Arg Asp Glu Val Phe Ala Ala Phe Gln Glu Ile Ser Asp Asn Phe
65 70 75 80
gga gat ttg aat gtt gtc gtc aat aac gct ggc gtt gct cca acc act 288
Gly Asp Leu Asn Val Val Val Asn Asn Ala Gly Val Ala Pro Thr Thr
85 90 95
cca att atg gaa gtc acc gaa gac gat atg gaa tgg acc tgg aaa atc 336
Pro Ile Met Glu Val Thr Glu Asp Asp Met Glu Trp Thr Trp Lys Ile
100 105 110
aac gtt gat ggt gtt gta tgg gga att caa gcg gct gcg acc att ttt 384
Asn Val Asp Gly Val Val Trp Gly Ile Gln Ala Ala Ala Thr Ile Phe
115 120 125
aag aaa ttc aat cac gct gga aaa atc atc aac gct acc tcc caa gcc 432
Lys Lys Phe Asn His Ala Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala
130 135 140
gga gtt caa ggc aat cca gat tta acc gct tac gga tcg aca aaa ttt 480
Gly Val Gln Gly Asn Pro Asp Leu Thr Ala Tyr Gly Ser Thr Lys Phe
145 150 155 160
gcg att cgt gga att act caa aca act gcc aaa gag ttg gct aaa ttt 528
Ala Ile Arg Gly Ile Thr Gln Thr Thr Ala Lys Glu Leu Ala Lys Phe
165 170 175
ggt att act gtt aat gct tac gca cct gga att gta aaa aca cca atg 576
Gly Ile Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Lys Thr Pro Met
180 185 190
atg gaa gac att gcg cac gaa gtc ggc aag aat gcc gga aaa gac gac 624
Met Glu Asp Ile Ala His Glu Val Gly Lys Asn Ala Gly Lys Asp Asp
195 200 205
gac tgg gga atg aat caa ttt gcc aaa gga atc gcc ttg ggt aga att 672
Asp Trp Gly Met Asn Gln Phe Ala Lys Gly Ile Ala Leu Gly Arg Ile
210 215 220
tcc gaa ccg gaa gat gtt gcc aat gca gtt ggc ttt cta gca agt tct 720
Ser Glu Pro Glu Asp Val Ala Asn Ala Val Gly Phe Leu Ala Ser Ser
225 230 235 240
gat tcg aat tat gtt acc ggt caa aca atc gtc gtt gat gga ggt atg 768
Asp Ser Asn Tyr Val Thr Gly Gln Thr Ile Val Val Asp Gly Gly Met
245 250 255
gtt ttc aac taa 780
Val Phe Asn
<210> 108
<211> 259
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 108
Met Ser Asn Lys Val Ala Ile Ile Thr Gly Ala Gly Gln Gly Ile Gly
1 5 10 15
Lys Ala Ile Ala Gln Arg Leu Ala Lys Asp Gly Phe Lys Thr Gly Leu
20 25 30
Ile Gly Arg His Leu Glu Lys Val Glu Ala Val Ala Lys Glu Leu Asn
35 40 45
Glu Glu Tyr Gly Ser Asp Thr Ala Ile Ala Lys Lys Ala Asp Val Ser
50 55 60
Lys Arg Asp Glu Val Phe Ala Ala Phe Gln Glu Ile Ser Asp Asn Phe
65 70 75 80
Gly Asp Leu Asn Val Val Val Asn Asn Ala Gly Val Ala Pro Thr Thr
85 90 95
Pro Ile Met Glu Val Thr Glu Asp Asp Met Glu Trp Thr Trp Lys Ile
100 105 110
Asn Val Asp Gly Val Val Trp Gly Ile Gln Ala Ala Ala Thr Ile Phe
115 120 125
Lys Lys Phe Asn His Ala Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala
130 135 140
Gly Val Gln Gly Asn Pro Asp Leu Thr Ala Tyr Gly Ser Thr Lys Phe
145 150 155 160
Ala Ile Arg Gly Ile Thr Gln Thr Thr Ala Lys Glu Leu Ala Lys Phe
165 170 175
Gly Ile Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Lys Thr Pro Met
180 185 190
Met Glu Asp Ile Ala His Glu Val Gly Lys Asn Ala Gly Lys Asp Asp
195 200 205
Asp Trp Gly Met Asn Gln Phe Ala Lys Gly Ile Ala Leu Gly Arg Ile
210 215 220
Ser Glu Pro Glu Asp Val Ala Asn Ala Val Gly Phe Leu Ala Ser Ser
225 230 235 240
Asp Ser Asn Tyr Val Thr Gly Gln Thr Ile Val Val Asp Gly Gly Met
245 250 255
Val Phe Asn
<210> 109
<211> 774
<212> DNA
<213> 酒类酒球菌（Oenococcus oeni）
<220>
<221> CDS
<222> (1)..(774)
<223> 酒类酒球菌 PSU-1; 基因 OEOE_1602 编码乙偶姻还原酶
<400> 109
atg aca aat aaa gtc gca att gtt acc ggt ggt ggt cag gga att ggc 48
Met Thr Asn Lys Val Ala Ile Val Thr Gly Gly Gly Gln Gly Ile Gly
1 5 10 15
cgt gga atc gct ctg cgc cta tca aaa gac gga tat tca gtg att att 96
Arg Gly Ile Ala Leu Arg Leu Ser Lys Asp Gly Tyr Ser Val Ile Ile
20 25 30
aat gga aga act cag gag aaa cta gac aaa gtt gct gag gag atc aaa 144
Asn Gly Arg Thr Gln Glu Lys Leu Asp Lys Val Ala Glu Glu Ile Lys
35 40 45
aac aac aac gga aag gtg att act ttt gtt gga gat gtc tca agc aag 192
Asn Asn Asn Gly Lys Val Ile Thr Phe Val Gly Asp Val Ser Ser Lys
50 55 60
tct gct aac gag cag tta gta aaa aaa gcc gaa tcc gaa ttt gga cgt 240
Ser Ala Asn Glu Gln Leu Val Lys Lys Ala Glu Ser Glu Phe Gly Arg
65 70 75 80
ttg gac gtt ttt gta gcc aac gct gga atc gat tgg gtt gat act atc 288
Leu Asp Val Phe Val Ala Asn Ala Gly Ile Asp Trp Val Asp Thr Ile
85 90 95
gag gga aca ccg gca gac caa gta gat aat gta ata aat att aat ttg 336
Glu Gly Thr Pro Ala Asp Gln Val Asp Asn Val Ile Asn Ile Asn Leu
100 105 110
aaa tcg caa att tgg gcc ttg caa acg gcg ccg aaa gtt atg cga aaa 384
Lys Ser Gln Ile Trp Ala Leu Gln Thr Ala Pro Lys Val Met Arg Lys
115 120 125
agt ggc gat gga ggt tca atc gtt ttg gct tca tca att gcc ggc atg 432
Ser Gly Asp Gly Gly Ser Ile Val Leu Ala Ser Ser Ile Ala Gly Met
130 135 140
gaa ggt ttc gag atg ctg gga atc tat tct gcg act aaa ttt gca act 480
Glu Gly Phe Glu Met Leu Gly Ile Tyr Ser Ala Thr Lys Phe Ala Thr
145 150 155 160
cgc gga ttg gtt caa gcg gca gca aaa gaa ttg gcc gtc gac aag att 528
Arg Gly Leu Val Gln Ala Ala Ala Lys Glu Leu Ala Val Asp Lys Ile
165 170 175
cgc gtc aat gcc tat aac cca gga att gtc ctg aca cca atg tgg gat 576
Arg Val Asn Ala Tyr Asn Pro Gly Ile Val Leu Thr Pro Met Trp Asp
180 185 190
gaa atc gat gcc cgt ttt gcc gag cgt aaa aac gtt ccc gtt gga tcg 624
Glu Ile Asp Ala Arg Phe Ala Glu Arg Lys Asn Val Pro Val Gly Ser
195 200 205
aca cgc cag gca ttt ata gat gga att cta ttg gga cgt ggt gaa gaa 672
Thr Arg Gln Ala Phe Ile Asp Gly Ile Leu Leu Gly Arg Gly Glu Glu
210 215 220
cct gaa gat gtt gct aaa ttg gtc agc ttc ttg tcc ggc gat gaa agt 720
Pro Glu Asp Val Ala Lys Leu Val Ser Phe Leu Ser Gly Asp Glu Ser
225 230 235 240
gat tat att acc ggt cag tca atc gcg gtc gat ggt gga att aaa ttt 768
Asp Tyr Ile Thr Gly Gln Ser Ile Ala Val Asp Gly Gly Ile Lys Phe
245 250 255
tct taa 774
Ser
<210> 110
<211> 257
<212> PRT
<213> 酒类酒球菌（Oenococcus oeni）
<400> 110
Met Thr Asn Lys Val Ala Ile Val Thr Gly Gly Gly Gln Gly Ile Gly
1 5 10 15
Arg Gly Ile Ala Leu Arg Leu Ser Lys Asp Gly Tyr Ser Val Ile Ile
20 25 30
Asn Gly Arg Thr Gln Glu Lys Leu Asp Lys Val Ala Glu Glu Ile Lys
35 40 45
Asn Asn Asn Gly Lys Val Ile Thr Phe Val Gly Asp Val Ser Ser Lys
50 55 60
Ser Ala Asn Glu Gln Leu Val Lys Lys Ala Glu Ser Glu Phe Gly Arg
65 70 75 80
Leu Asp Val Phe Val Ala Asn Ala Gly Ile Asp Trp Val Asp Thr Ile
85 90 95
Glu Gly Thr Pro Ala Asp Gln Val Asp Asn Val Ile Asn Ile Asn Leu
100 105 110
Lys Ser Gln Ile Trp Ala Leu Gln Thr Ala Pro Lys Val Met Arg Lys
115 120 125
Ser Gly Asp Gly Gly Ser Ile Val Leu Ala Ser Ser Ile Ala Gly Met
130 135 140
Glu Gly Phe Glu Met Leu Gly Ile Tyr Ser Ala Thr Lys Phe Ala Thr
145 150 155 160
Arg Gly Leu Val Gln Ala Ala Ala Lys Glu Leu Ala Val Asp Lys Ile
165 170 175
Arg Val Asn Ala Tyr Asn Pro Gly Ile Val Leu Thr Pro Met Trp Asp
180 185 190
Glu Ile Asp Ala Arg Phe Ala Glu Arg Lys Asn Val Pro Val Gly Ser
195 200 205
Thr Arg Gln Ala Phe Ile Asp Gly Ile Leu Leu Gly Arg Gly Glu Glu
210 215 220
Pro Glu Asp Val Ala Lys Leu Val Ser Phe Leu Ser Gly Asp Glu Ser
225 230 235 240
Asp Tyr Ile Thr Gly Gln Ser Ile Ala Val Asp Gly Gly Ile Lys Phe
245 250 255
Ser
<210> 111
<211> 774
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(774)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_1663 编码乙偶姻还原酶
<400> 111
atg tct aaa gtt gca att gta aca gga tca gct ggt ggg tta gga aaa 48
Met Ser Lys Val Ala Ile Val Thr Gly Ser Ala Gly Gly Leu Gly Lys
1 5 10 15
ggc att gca gaa aga tta tgc tca gat ggt ttt agt gtt gtt gtt cat 96
Gly Ile Ala Glu Arg Leu Cys Ser Asp Gly Phe Ser Val Val Val His
20 25 30
gat att aac gaa caa cta tta aat gaa acc gtc aac gaa ttt aaa aat 144
Asp Ile Asn Glu Gln Leu Leu Asn Glu Thr Val Asn Glu Phe Lys Asn
35 40 45
aaa ggt tat gat gtt att ggc gtc aaa ggt gac gtg tct aaa cga aat 192
Lys Gly Tyr Asp Val Ile Gly Val Lys Gly Asp Val Ser Lys Arg Asn
50 55 60
gac caa ttt aat ctt gtt aaa aaa ggt gtt gag aag ttt ggc cat tta 240
Asp Gln Phe Asn Leu Val Lys Lys Gly Val Glu Lys Phe Gly His Leu
65 70 75 80
gat gta ttt gta aat aat gct gga att gat gca gta tcc cca ttc tta 288
Asp Val Phe Val Asn Asn Ala Gly Ile Asp Ala Val Ser Pro Phe Leu
85 90 95
gaa att aca gag gaa cag ctg aat aag ctc ttt agt att aat gta aat 336
Glu Ile Thr Glu Glu Gln Leu Asn Lys Leu Phe Ser Ile Asn Val Asn
100 105 110
ggc gtt gtt ttt gga aca caa gca gca gcc gaa caa ttt ata agc cag 384
Gly Val Val Phe Gly Thr Gln Ala Ala Ala Glu Gln Phe Ile Ser Gln
115 120 125
aaa tca aaa ggg aaa att atc aac gca tgc agt ata gct gga cat gaa 432
Lys Ser Lys Gly Lys Ile Ile Asn Ala Cys Ser Ile Ala Gly His Glu
130 135 140
tcc tac gaa atg ttg ggt aca tac tca gct acg aaa cac gca gtt aaa 480
Ser Tyr Glu Met Leu Gly Thr Tyr Ser Ala Thr Lys His Ala Val Lys
145 150 155 160
tcc ttt act cat tcc gct gcc aaa gaa tta gcc aaa tat caa att aca 528
Ser Phe Thr His Ser Ala Ala Lys Glu Leu Ala Lys Tyr Gln Ile Thr
165 170 175
gtg aat gca tac tgc cct ggt gtc gct aag acg aaa atg tgg gat cga 576
Val Asn Ala Tyr Cys Pro Gly Val Ala Lys Thr Lys Met Trp Asp Arg
180 185 190
att gat gaa gaa atg gtc aaa tat agt gat gat tta aaa cct ggt gaa 624
Ile Asp Glu Glu Met Val Lys Tyr Ser Asp Asp Leu Lys Pro Gly Glu
195 200 205
gca ttt gaa aag ttt tct tct gaa ata gca tta gga aga tat caa acc 672
Ala Phe Glu Lys Phe Ser Ser Glu Ile Ala Leu Gly Arg Tyr Gln Thr
210 215 220
ccg gaa gat gta gca aac tta gtt tca ttc tta gca tca gat gac gct 720
Pro Glu Asp Val Ala Asn Leu Val Ser Phe Leu Ala Ser Asp Asp Ala
225 230 235 240
gat tat ata aca gga caa gca att tta aca gat ggt gga ctt gta tat 768
Asp Tyr Ile Thr Gly Gln Ala Ile Leu Thr Asp Gly Gly Leu Val Tyr
245 250 255
aga taa 774
Arg
<210> 112
<211> 257
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 112
Met Ser Lys Val Ala Ile Val Thr Gly Ser Ala Gly Gly Leu Gly Lys
1 5 10 15
Gly Ile Ala Glu Arg Leu Cys Ser Asp Gly Phe Ser Val Val Val His
20 25 30
Asp Ile Asn Glu Gln Leu Leu Asn Glu Thr Val Asn Glu Phe Lys Asn
35 40 45
Lys Gly Tyr Asp Val Ile Gly Val Lys Gly Asp Val Ser Lys Arg Asn
50 55 60
Asp Gln Phe Asn Leu Val Lys Lys Gly Val Glu Lys Phe Gly His Leu
65 70 75 80
Asp Val Phe Val Asn Asn Ala Gly Ile Asp Ala Val Ser Pro Phe Leu
85 90 95
Glu Ile Thr Glu Glu Gln Leu Asn Lys Leu Phe Ser Ile Asn Val Asn
100 105 110
Gly Val Val Phe Gly Thr Gln Ala Ala Ala Glu Gln Phe Ile Ser Gln
115 120 125
Lys Ser Lys Gly Lys Ile Ile Asn Ala Cys Ser Ile Ala Gly His Glu
130 135 140
Ser Tyr Glu Met Leu Gly Thr Tyr Ser Ala Thr Lys His Ala Val Lys
145 150 155 160
Ser Phe Thr His Ser Ala Ala Lys Glu Leu Ala Lys Tyr Gln Ile Thr
165 170 175
Val Asn Ala Tyr Cys Pro Gly Val Ala Lys Thr Lys Met Trp Asp Arg
180 185 190
Ile Asp Glu Glu Met Val Lys Tyr Ser Asp Asp Leu Lys Pro Gly Glu
195 200 205
Ala Phe Glu Lys Phe Ser Ser Glu Ile Ala Leu Gly Arg Tyr Gln Thr
210 215 220
Pro Glu Asp Val Ala Asn Leu Val Ser Phe Leu Ala Ser Asp Asp Ala
225 230 235 240
Asp Tyr Ile Thr Gly Gln Ala Ile Leu Thr Asp Gly Gly Leu Val Tyr
245 250 255
Arg
<210> 113
<211> 981
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(981)
<223> 乳酸乳球菌乳脂亚种 MG1363; 基因 llmg_1642
编码2,3-丁二醇脱氢酶
<220>
<221> misc_feature（新的或少见的特征）
<222> (1)..(3)
<223> 由起始密码子处的tRNA插入Met而不是Val
<400> 113
gtg gct tgg tgt gga atc tgt ggt aca gat ttg cac gaa ttt tta gat 48
Val Ala Trp Cys Gly Ile Cys Gly Thr Asp Leu His Glu Phe Leu Asp
1 5 10 15
gga cca att ttt tgt cca tca gca gaa cat cca aat cct att aca ggt 96
Gly Pro Ile Phe Cys Pro Ser Ala Glu His Pro Asn Pro Ile Thr Gly
20 25 30
gaa gtt cca cca gtt act ctc gga cat gaa atg tct ggt att gta aac 144
Glu Val Pro Pro Val Thr Leu Gly His Glu Met Ser Gly Ile Val Asn
35 40 45
ttt gta ggt caa ggg gta agt ggg ctc aaa gta ggt gac cat gtc gtt 192
Phe Val Gly Gln Gly Val Ser Gly Leu Lys Val Gly Asp His Val Val
50 55 60
gtt gaa cct tat atc gtt ccc gaa ggt act gat aca agt gaa act gga 240
Val Glu Pro Tyr Ile Val Pro Glu Gly Thr Asp Thr Ser Glu Thr Gly
65 70 75 80
cat tac aat ctc tca gaa gga tca aac ttt att ggt ttg ggc gga aat 288
His Tyr Asn Leu Ser Glu Gly Ser Asn Phe Ile Gly Leu Gly Gly Asn
85 90 95
ggc ggc ggt ctg gct gaa aaa att tca gtt gat gaa cgt tgg gta cat 336
Gly Gly Gly Leu Ala Glu Lys Ile Ser Val Asp Glu Arg Trp Val His
100 105 110
aaa att cct gac gat tta cca tta gat gaa gca gca cta att gaa cca 384
Lys Ile Pro Asp Asp Leu Pro Leu Asp Glu Ala Ala Leu Ile Glu Pro
115 120 125
ctt tct gtg gga tat cat gct gtt gaa cgt gct aat ttg agt gaa aag 432
Leu Ser Val Gly Tyr His Ala Val Glu Arg Ala Asn Leu Ser Glu Lys
130 135 140
agt acg gta cta gtt gtt gga gct ggt cca atc gga ctt ttg aca gct 480
Ser Thr Val Leu Val Val Gly Ala Gly Pro Ile Gly Leu Leu Thr Ala
145 150 155 160
gca gtt gcc aaa gct caa ggg cat aca gta att att agt gag cct agt 528
Ala Val Ala Lys Ala Gln Gly His Thr Val Ile Ile Ser Glu Pro Ser
165 170 175
ggt ttg cgt cgt aaa aaa gct caa gaa gca gaa gtt gct gat tat ttc 576
Gly Leu Arg Arg Lys Lys Ala Gln Glu Ala Glu Val Ala Asp Tyr Phe
180 185 190
ttt aat cca att gaa gat gat att cag gca aaa gtt cat caa att aat 624
Phe Asn Pro Ile Glu Asp Asp Ile Gln Ala Lys Val His Gln Ile Asn
195 200 205
gaa aaa gga gtt gat gct gct ttt gaa tgt act tct gtt caa cca gga 672
Glu Lys Gly Val Asp Ala Ala Phe Glu Cys Thr Ser Val Gln Pro Gly
210 215 220
ttt gat gcg tgt tta gat tct att cgc atg ggt gga aca gtt gtt atc 720
Phe Asp Ala Cys Leu Asp Ser Ile Arg Met Gly Gly Thr Val Val Ile
225 230 235 240
gtt gca att tgg ggt aaa cca gct agt gtt gac atg gca aaa tta gtc 768
Val Ala Ile Trp Gly Lys Pro Ala Ser Val Asp Met Ala Lys Leu Val
245 250 255
att aaa gaa gct aat ctt tta ggg act att gct tat aat aat act cat 816
Ile Lys Glu Ala Asn Leu Leu Gly Thr Ile Ala Tyr Asn Asn Thr His
260 265 270
cct aaa acc att gat tta gtt gca act ggt aag att aaa ttg aat caa 864
Pro Lys Thr Ile Asp Leu Val Ala Thr Gly Lys Ile Lys Leu Asn Gln
275 280 285
ttt att aca ggt aaa att ggc tta gat gac ctg att gat aaa ggt ttt 912
Phe Ile Thr Gly Lys Ile Gly Leu Asp Asp Leu Ile Asp Lys Gly Phe
290 295 300
gat act ttg att cat cac aat gaa aca gca gtt aag att tta gtt tca 960
Asp Thr Leu Ile His His Asn Glu Thr Ala Val Lys Ile Leu Val Ser
305 310 315 320
cca act gga aaa ggt cta taa 981
Pro Thr Gly Lys Gly Leu
325
<210> 114
<211> 326
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 114
Val Ala Trp Cys Gly Ile Cys Gly Thr Asp Leu His Glu Phe Leu Asp
1 5 10 15
Gly Pro Ile Phe Cys Pro Ser Ala Glu His Pro Asn Pro Ile Thr Gly
20 25 30
Glu Val Pro Pro Val Thr Leu Gly His Glu Met Ser Gly Ile Val Asn
35 40 45
Phe Val Gly Gln Gly Val Ser Gly Leu Lys Val Gly Asp His Val Val
50 55 60
Val Glu Pro Tyr Ile Val Pro Glu Gly Thr Asp Thr Ser Glu Thr Gly
65 70 75 80
His Tyr Asn Leu Ser Glu Gly Ser Asn Phe Ile Gly Leu Gly Gly Asn
85 90 95
Gly Gly Gly Leu Ala Glu Lys Ile Ser Val Asp Glu Arg Trp Val His
100 105 110
Lys Ile Pro Asp Asp Leu Pro Leu Asp Glu Ala Ala Leu Ile Glu Pro
115 120 125
Leu Ser Val Gly Tyr His Ala Val Glu Arg Ala Asn Leu Ser Glu Lys
130 135 140
Ser Thr Val Leu Val Val Gly Ala Gly Pro Ile Gly Leu Leu Thr Ala
145 150 155 160
Ala Val Ala Lys Ala Gln Gly His Thr Val Ile Ile Ser Glu Pro Ser
165 170 175
Gly Leu Arg Arg Lys Lys Ala Gln Glu Ala Glu Val Ala Asp Tyr Phe
180 185 190
Phe Asn Pro Ile Glu Asp Asp Ile Gln Ala Lys Val His Gln Ile Asn
195 200 205
Glu Lys Gly Val Asp Ala Ala Phe Glu Cys Thr Ser Val Gln Pro Gly
210 215 220
Phe Asp Ala Cys Leu Asp Ser Ile Arg Met Gly Gly Thr Val Val Ile
225 230 235 240
Val Ala Ile Trp Gly Lys Pro Ala Ser Val Asp Met Ala Lys Leu Val
245 250 255
Ile Lys Glu Ala Asn Leu Leu Gly Thr Ile Ala Tyr Asn Asn Thr His
260 265 270
Pro Lys Thr Ile Asp Leu Val Ala Thr Gly Lys Ile Lys Leu Asn Gln
275 280 285
Phe Ile Thr Gly Lys Ile Gly Leu Asp Asp Leu Ile Asp Lys Gly Phe
290 295 300
Asp Thr Leu Ile His His Asn Glu Thr Ala Val Lys Ile Leu Val Ser
305 310 315 320
Pro Thr Gly Lys Gly Leu
325
<210> 115
<211> 1341
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(1341)
<223> 乳酸乳球菌乳脂亚种 MG1363; 基因 llmg_0408
编码H2O形成NADH氧化酶
<400> 115
atg aaa atc gta gtt atc ggt aca aac cac gca ggc att gct aca gcg 48
Met Lys Ile Val Val Ile Gly Thr Asn His Ala Gly Ile Ala Thr Ala
1 5 10 15
aat aca tta ctt gaa caa tat ccc ggg cat gaa att gtc atg att gac 96
Asn Thr Leu Leu Glu Gln Tyr Pro Gly His Glu Ile Val Met Ile Asp
20 25 30
cgt aat agc aac atg agt tat cta ggt tgt ggc aca gca att tgg gtt 144
Arg Asn Ser Asn Met Ser Tyr Leu Gly Cys Gly Thr Ala Ile Trp Val
35 40 45
gga aga caa att gaa aaa cca gat gaa tta ttt tat gcc aaa gca gag 192
Gly Arg Gln Ile Glu Lys Pro Asp Glu Leu Phe Tyr Ala Lys Ala Glu
50 55 60
gat ttt gag gca aaa ggg gta aaa att ttg act gaa aca gaa gtt tca 240
Asp Phe Glu Ala Lys Gly Val Lys Ile Leu Thr Glu Thr Glu Val Ser
65 70 75 80
gaa att gat ttt gct aat aag aaa gtt tat gca aaa act aaa tct gat 288
Glu Ile Asp Phe Ala Asn Lys Lys Val Tyr Ala Lys Thr Lys Ser Asp
85 90 95
gat gaa ata att gaa gct tac gac aag ctt gtt tta gca aca ggt tca 336
Asp Glu Ile Ile Glu Ala Tyr Asp Lys Leu Val Leu Ala Thr Gly Ser
100 105 110
cgt cca att att cct aat cta cca ggc aaa gac ctt aag gga att cat 384
Arg Pro Ile Ile Pro Asn Leu Pro Gly Lys Asp Leu Lys Gly Ile His
115 120 125
ttt ctg aaa ctt ttt caa gaa ggt caa gca att gac gca gaa ttt gcc 432
Phe Leu Lys Leu Phe Gln Glu Gly Gln Ala Ile Asp Ala Glu Phe Ala
130 135 140
aaa gaa aaa gtc aag cgt atc gca gtc att ggt gca gga tat atc ggt 480
Lys Glu Lys Val Lys Arg Ile Ala Val Ile Gly Ala Gly Tyr Ile Gly
145 150 155 160
aca gag att gcg gaa gca gct aaa cgt cgg ggt aaa gaa gtt ctt ctc 528
Thr Glu Ile Ala Glu Ala Ala Lys Arg Arg Gly Lys Glu Val Leu Leu
165 170 175
ttt gac gct gaa aat act tca ctt gca tca tat tat gat gaa gaa ttt 576
Phe Asp Ala Glu Asn Thr Ser Leu Ala Ser Tyr Tyr Asp Glu Glu Phe
180 185 190
gcc aaa gga atg gat gaa aac ctt gct caa cat gga att gaa ctt cat 624
Ala Lys Gly Met Asp Glu Asn Leu Ala Gln His Gly Ile Glu Leu His
195 200 205
ttt gga gaa ctg gcc aaa gaa ttt aaa gcg aat gag gaa ggt tat gta 672
Phe Gly Glu Leu Ala Lys Glu Phe Lys Ala Asn Glu Glu Gly Tyr Val
210 215 220
tca caa atc gta acc aac aag gcg act tat gat gtt gat ctt gtc atc 720
Ser Gln Ile Val Thr Asn Lys Ala Thr Tyr Asp Val Asp Leu Val Ile
225 230 235 240
aat tgt att ggt ttt act gcc aac agt gcc ttg gca agt gat aag tta 768
Asn Cys Ile Gly Phe Thr Ala Asn Ser Ala Leu Ala Ser Asp Lys Leu
245 250 255
gct acc ttc aaa aat ggc gca atc aag gtg gat aag cat caa caa agt 816
Ala Thr Phe Lys Asn Gly Ala Ile Lys Val Asp Lys His Gln Gln Ser
260 265 270
agt gat cca gat gtt tac gcg gta ggt gat gtt gcg aca att tat tct 864
Ser Asp Pro Asp Val Tyr Ala Val Gly Asp Val Ala Thr Ile Tyr Ser
275 280 285
aat gcc ttg caa gat ttt act tat atc gct ctt gcc tca aac gct gtt 912
Asn Ala Leu Gln Asp Phe Thr Tyr Ile Ala Leu Ala Ser Asn Ala Val
290 295 300
cgg tca gga att gtc gca gga cac aat att ggt gga aaa gaa tta gaa 960
Arg Ser Gly Ile Val Ala Gly His Asn Ile Gly Gly Lys Glu Leu Glu
305 310 315 320
tct gtt ggt gtt caa ggt tct aat ggt att tcg att ttt ggt tac aat 1008
Ser Val Gly Val Gln Gly Ser Asn Gly Ile Ser Ile Phe Gly Tyr Asn
325 330 335
atg act tct aca gga ctt tct gtt aaa gct gct aaa aaa tta ggt tta 1056
Met Thr Ser Thr Gly Leu Ser Val Lys Ala Ala Lys Lys Leu Gly Leu
340 345 350
gaa gtt tca ttt agt gat ttt gaa gat aaa caa aaa gct tgg ttt ctt 1104
Glu Val Ser Phe Ser Asp Phe Glu Asp Lys Gln Lys Ala Trp Phe Leu
355 360 365
cat gaa aac aac gat agt gtg aaa att cgt atc gta tat gag aca aaa 1152
His Glu Asn Asn Asp Ser Val Lys Ile Arg Ile Val Tyr Glu Thr Lys
370 375 380
agt cgc aga att att gga gca caa ctt gct agt aaa agt gag ata att 1200
Ser Arg Arg Ile Ile Gly Ala Gln Leu Ala Ser Lys Ser Glu Ile Ile
385 390 395 400
gca gga aat ata aat atg ttc agt tta gcg att caa gag aaa aaa aca 1248
Ala Gly Asn Ile Asn Met Phe Ser Leu Ala Ile Gln Glu Lys Lys Thr
405 410 415
att gat gaa cta gct ttg ctt gat tta ttc ttt ctc ccc cac ttc aac 1296
Ile Asp Glu Leu Ala Leu Leu Asp Leu Phe Phe Leu Pro His Phe Asn
420 425 430
agt cca tat aat tat atg aca gtt gca gct ttg aat gcc aaa taa 1341
Ser Pro Tyr Asn Tyr Met Thr Val Ala Ala Leu Asn Ala Lys
435 440 445
<210> 116
<211> 446
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 116
Met Lys Ile Val Val Ile Gly Thr Asn His Ala Gly Ile Ala Thr Ala
1 5 10 15
Asn Thr Leu Leu Glu Gln Tyr Pro Gly His Glu Ile Val Met Ile Asp
20 25 30
Arg Asn Ser Asn Met Ser Tyr Leu Gly Cys Gly Thr Ala Ile Trp Val
35 40 45
Gly Arg Gln Ile Glu Lys Pro Asp Glu Leu Phe Tyr Ala Lys Ala Glu
50 55 60
Asp Phe Glu Ala Lys Gly Val Lys Ile Leu Thr Glu Thr Glu Val Ser
65 70 75 80
Glu Ile Asp Phe Ala Asn Lys Lys Val Tyr Ala Lys Thr Lys Ser Asp
85 90 95
Asp Glu Ile Ile Glu Ala Tyr Asp Lys Leu Val Leu Ala Thr Gly Ser
100 105 110
Arg Pro Ile Ile Pro Asn Leu Pro Gly Lys Asp Leu Lys Gly Ile His
115 120 125
Phe Leu Lys Leu Phe Gln Glu Gly Gln Ala Ile Asp Ala Glu Phe Ala
130 135 140
Lys Glu Lys Val Lys Arg Ile Ala Val Ile Gly Ala Gly Tyr Ile Gly
145 150 155 160
Thr Glu Ile Ala Glu Ala Ala Lys Arg Arg Gly Lys Glu Val Leu Leu
165 170 175
Phe Asp Ala Glu Asn Thr Ser Leu Ala Ser Tyr Tyr Asp Glu Glu Phe
180 185 190
Ala Lys Gly Met Asp Glu Asn Leu Ala Gln His Gly Ile Glu Leu His
195 200 205
Phe Gly Glu Leu Ala Lys Glu Phe Lys Ala Asn Glu Glu Gly Tyr Val
210 215 220
Ser Gln Ile Val Thr Asn Lys Ala Thr Tyr Asp Val Asp Leu Val Ile
225 230 235 240
Asn Cys Ile Gly Phe Thr Ala Asn Ser Ala Leu Ala Ser Asp Lys Leu
245 250 255
Ala Thr Phe Lys Asn Gly Ala Ile Lys Val Asp Lys His Gln Gln Ser
260 265 270
Ser Asp Pro Asp Val Tyr Ala Val Gly Asp Val Ala Thr Ile Tyr Ser
275 280 285
Asn Ala Leu Gln Asp Phe Thr Tyr Ile Ala Leu Ala Ser Asn Ala Val
290 295 300
Arg Ser Gly Ile Val Ala Gly His Asn Ile Gly Gly Lys Glu Leu Glu
305 310 315 320
Ser Val Gly Val Gln Gly Ser Asn Gly Ile Ser Ile Phe Gly Tyr Asn
325 330 335
Met Thr Ser Thr Gly Leu Ser Val Lys Ala Ala Lys Lys Leu Gly Leu
340 345 350
Glu Val Ser Phe Ser Asp Phe Glu Asp Lys Gln Lys Ala Trp Phe Leu
355 360 365
His Glu Asn Asn Asp Ser Val Lys Ile Arg Ile Val Tyr Glu Thr Lys
370 375 380
Ser Arg Arg Ile Ile Gly Ala Gln Leu Ala Ser Lys Ser Glu Ile Ile
385 390 395 400
Ala Gly Asn Ile Asn Met Phe Ser Leu Ala Ile Gln Glu Lys Lys Thr
405 410 415
Ile Asp Glu Leu Ala Leu Leu Asp Leu Phe Phe Leu Pro His Phe Asn
420 425 430
Ser Pro Tyr Asn Tyr Met Thr Val Ala Ala Leu Asn Ala Lys
435 440 445
<210> 117
<211> 981
<212> DNA
<213> 干酪乳杆菌（Lactobacillus casei）
<220>
<221> CDS
<222> (1)..(981)
<223> 干酪乳杆菌 ATCC 334; 基因 LSEI_2549 编码形成H2O的NADH氧化酶
<220>
<221> misc_feature（新的或少见的特征）
<222> (1)..(3)
<223> 由起始密码子处的tRNA插入Met而不是Val
<400> 117
gtg gca agt att acg gat aag gat cac caa aaa gtt att ctc gtt ggt 48
Val Ala Ser Ile Thr Asp Lys Asp His Gln Lys Val Ile Leu Val Gly
1 5 10 15
gac ggc gcc gtt ggt tca agt tat gcc tac gca atg gtt ttg caa ggt 96
Asp Gly Ala Val Gly Ser Ser Tyr Ala Tyr Ala Met Val Leu Gln Gly
20 25 30
atc gct cag gaa atc gga atc gtt gac att ttc aag gac aag aca aag 144
Ile Ala Gln Glu Ile Gly Ile Val Asp Ile Phe Lys Asp Lys Thr Lys
35 40 45
ggt gac gcg att gac ttg agc aac gcg ctc cca ttc aca agt cct aag 192
Gly Asp Ala Ile Asp Leu Ser Asn Ala Leu Pro Phe Thr Ser Pro Lys
50 55 60
aag att tat tca gct gaa tac agc gat gct aag gat gct gat ctg gtt 240
Lys Ile Tyr Ser Ala Glu Tyr Ser Asp Ala Lys Asp Ala Asp Leu Val
65 70 75 80
gtt atc aca gct ggc gct cct cag aag cct ggc gaa act cgt ttg gac 288
Val Ile Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp
85 90 95
ttg gtt aac aag aac ttg aag atc ttg aag tcc att gtt gac cca atc 336
Leu Val Asn Lys Asn Leu Lys Ile Leu Lys Ser Ile Val Asp Pro Ile
100 105 110
gtt gat tcc ggc ttt aac ggt att ttc tta gtt gct gcc aac cca gtt 384
Val Asp Ser Gly Phe Asn Gly Ile Phe Leu Val Ala Ala Asn Pro Val
115 120 125
gac att ttg acc tat gca act tgg aaa ctt tct ggc ttc ccg aag aac 432
Asp Ile Leu Thr Tyr Ala Thr Trp Lys Leu Ser Gly Phe Pro Lys Asn
130 135 140
cgg gtt gtt ggt tcc ggt act tca ctg gac acc gcc cgc ttc cgt cag 480
Arg Val Val Gly Ser Gly Thr Ser Leu Asp Thr Ala Arg Phe Arg Gln
145 150 155 160
tcc att gct gaa atg gtt aat gtt gac gct cgt tcg gtc cac gct tac 528
Ser Ile Ala Glu Met Val Asn Val Asp Ala Arg Ser Val His Ala Tyr
165 170 175
atc atg ggc gaa cat ggt gac act gaa ttc cct gta tgg tcc cac gct 576
Ile Met Gly Glu His Gly Asp Thr Glu Phe Pro Val Trp Ser His Ala
180 185 190
aac att ggt ggc gtt acc atc gct gaa tgg gtt aag gct cat cca gaa 624
Asn Ile Gly Gly Val Thr Ile Ala Glu Trp Val Lys Ala His Pro Glu
195 200 205
atc aag gaa gac aag ctt gtt aag atg ttt gaa gac gtt cgt gac gcc 672
Ile Lys Glu Asp Lys Leu Val Lys Met Phe Glu Asp Val Arg Asp Ala
210 215 220
gct tat gaa atc atc aaa ctc aag ggt gcg acc ttc tat ggt atc gca 720
Ala Tyr Glu Ile Ile Lys Leu Lys Gly Ala Thr Phe Tyr Gly Ile Ala
225 230 235 240
act gcc ctt gcc cgg att tca aag gca atc ctt aac gac gaa aat gcg 768
Thr Ala Leu Ala Arg Ile Ser Lys Ala Ile Leu Asn Asp Glu Asn Ala
245 250 255
gtt ctg cca ctt tcc gtt tac atg gat ggt caa tat ggc ttg aac gac 816
Val Leu Pro Leu Ser Val Tyr Met Asp Gly Gln Tyr Gly Leu Asn Asp
260 265 270
atc tac atc ggt act cca gct gtg atc aac cgt aat ggt atc cag aac 864
Ile Tyr Ile Gly Thr Pro Ala Val Ile Asn Arg Asn Gly Ile Gln Asn
275 280 285
atc ctg gaa atc cca ttg acc gat cac gaa gaa gaa tcc atg cag aaa 912
Ile Leu Glu Ile Pro Leu Thr Asp His Glu Glu Glu Ser Met Gln Lys
290 295 300
tct gct tct caa ttg aag aag gtt ctg acc gat gct ttc gct aag aat 960
Ser Ala Ser Gln Leu Lys Lys Val Leu Thr Asp Ala Phe Ala Lys Asn
305 310 315 320
gac atc gaa act cgt cag taa 981
Asp Ile Glu Thr Arg Gln
325
<210> 118
<211> 326
<212> PRT
<213> 干酪乳杆菌（Lactobacillus casei）
<400> 118
Val Ala Ser Ile Thr Asp Lys Asp His Gln Lys Val Ile Leu Val Gly
1 5 10 15
Asp Gly Ala Val Gly Ser Ser Tyr Ala Tyr Ala Met Val Leu Gln Gly
20 25 30
Ile Ala Gln Glu Ile Gly Ile Val Asp Ile Phe Lys Asp Lys Thr Lys
35 40 45
Gly Asp Ala Ile Asp Leu Ser Asn Ala Leu Pro Phe Thr Ser Pro Lys
50 55 60
Lys Ile Tyr Ser Ala Glu Tyr Ser Asp Ala Lys Asp Ala Asp Leu Val
65 70 75 80
Val Ile Thr Ala Gly Ala Pro Gln Lys Pro Gly Glu Thr Arg Leu Asp
85 90 95
Leu Val Asn Lys Asn Leu Lys Ile Leu Lys Ser Ile Val Asp Pro Ile
100 105 110
Val Asp Ser Gly Phe Asn Gly Ile Phe Leu Val Ala Ala Asn Pro Val
115 120 125
Asp Ile Leu Thr Tyr Ala Thr Trp Lys Leu Ser Gly Phe Pro Lys Asn
130 135 140
Arg Val Val Gly Ser Gly Thr Ser Leu Asp Thr Ala Arg Phe Arg Gln
145 150 155 160
Ser Ile Ala Glu Met Val Asn Val Asp Ala Arg Ser Val His Ala Tyr
165 170 175
Ile Met Gly Glu His Gly Asp Thr Glu Phe Pro Val Trp Ser His Ala
180 185 190
Asn Ile Gly Gly Val Thr Ile Ala Glu Trp Val Lys Ala His Pro Glu
195 200 205
Ile Lys Glu Asp Lys Leu Val Lys Met Phe Glu Asp Val Arg Asp Ala
210 215 220
Ala Tyr Glu Ile Ile Lys Leu Lys Gly Ala Thr Phe Tyr Gly Ile Ala
225 230 235 240
Thr Ala Leu Ala Arg Ile Ser Lys Ala Ile Leu Asn Asp Glu Asn Ala
245 250 255
Val Leu Pro Leu Ser Val Tyr Met Asp Gly Gln Tyr Gly Leu Asn Asp
260 265 270
Ile Tyr Ile Gly Thr Pro Ala Val Ile Asn Arg Asn Gly Ile Gln Asn
275 280 285
Ile Leu Glu Ile Pro Leu Thr Asp His Glu Glu Glu Ser Met Gln Lys
290 295 300
Ser Ala Ser Gln Leu Lys Lys Val Leu Thr Asp Ala Phe Ala Lys Asn
305 310 315 320
Asp Ile Glu Thr Arg Gln
325
<210> 119
<211> 1413
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(1413)
<223> 植物乳杆菌 WCFS1; 基因 lp_0760 e编码形成H2O的NADH氧化酶
<400> 119
atg aag att gta att gtt gga tgt acg cat gcg ggg acc gct gcg gct 48
Met Lys Ile Val Ile Val Gly Cys Thr His Ala Gly Thr Ala Ala Ala
1 5 10 15
gca caa att ctg aag gat cat ccg gag gct acg gtc acg att tat gag 96
Ala Gln Ile Leu Lys Asp His Pro Glu Ala Thr Val Thr Ile Tyr Glu
20 25 30
cgt gat gac aat att tca ttc tta tca tgt ggg atc tac ctt tat ctc 144
Arg Asp Asp Asn Ile Ser Phe Leu Ser Cys Gly Ile Tyr Leu Tyr Leu
35 40 45
ggg gga aag gtc aat cac ttg gaa gat atg ttc tat tcg tca cca gaa 192
Gly Gly Lys Val Asn His Leu Glu Asp Met Phe Tyr Ser Ser Pro Glu
50 55 60
acg tta gcc aag ttg ggg gca atg gtc aga acc aaa cat aac gtg tta 240
Thr Leu Ala Lys Leu Gly Ala Met Val Arg Thr Lys His Asn Val Leu
65 70 75 80
aaa att gac gcc cag tcg aag acc tta caa gtg gtt gac atg act act 288
Lys Ile Asp Ala Gln Ser Lys Thr Leu Gln Val Val Asp Met Thr Thr
85 90 95
gga aaa gtg ttt ggc gat act tat gat aaa ttg att atg acg act ggt 336
Gly Lys Val Phe Gly Asp Thr Tyr Asp Lys Leu Ile Met Thr Thr Gly
100 105 110
tca aat gtt gcg gtc ccg ccg att ttt gga atc gat gag tct aag gtc 384
Ser Asn Val Ala Val Pro Pro Ile Phe Gly Ile Asp Glu Ser Lys Val
115 120 125
cta tta tgc aag acc tac agc caa gca cag gaa att tac caa aca gcc 432
Leu Leu Cys Lys Thr Tyr Ser Gln Ala Gln Glu Ile Tyr Gln Thr Ala
130 135 140
caa gac aat cag cgg atc gca atc att ggg gcg ggt tat att gga acc 480
Gln Asp Asn Gln Arg Ile Ala Ile Ile Gly Ala Gly Tyr Ile Gly Thr
145 150 155 160
gaa tta tct gaa agt tat gca aat aca cat cat gaa gta acg tta ttc 528
Glu Leu Ser Glu Ser Tyr Ala Asn Thr His His Glu Val Thr Leu Phe
165 170 175
caa tcg cat gat caa att ttg aat aat tac att agt aaa gaa att tca 576
Gln Ser His Asp Gln Ile Leu Asn Asn Tyr Ile Ser Lys Glu Ile Ser
180 185 190
gac caa gca gtt cag cta tta aaa gat cat ggc gtg aac gtg cta ttg 624
Asp Gln Ala Val Gln Leu Leu Lys Asp His Gly Val Asn Val Leu Leu
195 200 205
aat cac caa gta act gcc ttt act ggt aat gat gat ggt gaa ctt gtc 672
Asn His Gln Val Thr Ala Phe Thr Gly Asn Asp Asp Gly Glu Leu Val
210 215 220
att gaa acc aat caa ggc gat ttt aat gct gat cta gca att gtc gga 720
Ile Glu Thr Asn Gln Gly Asp Phe Asn Ala Asp Leu Ala Ile Val Gly
225 230 235 240
act ggg ttt gtt ccc aat aca gaa ctg tta cgc ggt caa gtt gag atg 768
Thr Gly Phe Val Pro Asn Thr Glu Leu Leu Arg Gly Gln Val Glu Met
245 250 255
gat aag cac ggt gcc att atc atc aat gat tac gtt cag acc tct gac 816
Asp Lys His Gly Ala Ile Ile Ile Asn Asp Tyr Val Gln Thr Ser Asp
260 265 270
cca gat att tac gct gct ggt gat tct tgt gtc gtt aac ttc aac cca 864
Pro Asp Ile Tyr Ala Ala Gly Asp Ser Cys Val Val Asn Phe Asn Pro
275 280 285
act ggg cgt tcg gct tat acg cca tta gca acc aat gcg gtt cgg cag 912
Thr Gly Arg Ser Ala Tyr Thr Pro Leu Ala Thr Asn Ala Val Arg Gln
290 295 300
ggt gcg tta gcg ggg gcc aat gtt ttt ggt aac cag caa cga tat atg 960
Gly Ala Leu Ala Gly Ala Asn Val Phe Gly Asn Gln Gln Arg Tyr Met
305 310 315 320
ggg act caa gcg aca tcg gcc atg caa ctc ttc gat cac acg ctg gca 1008
Gly Thr Gln Ala Thr Ser Ala Met Gln Leu Phe Asp His Thr Leu Ala
325 330 335
acc acg ggg tta aca ctg gca atg gcg aaa ttg aat cat att cca gct 1056
Thr Thr Gly Leu Thr Leu Ala Met Ala Lys Leu Asn His Ile Pro Ala
340 345 350
gaa cgg gtg act tat gag ggc aac tgg cga cca gct tac atg cca agt 1104
Glu Arg Val Thr Tyr Glu Gly Asn Trp Arg Pro Ala Tyr Met Pro Ser
355 360 365
acg gcg aaa ctg acg att gaa tta att tat aat ccg gaa aat cgt aaa 1152
Thr Ala Lys Leu Thr Ile Glu Leu Ile Tyr Asn Pro Glu Asn Arg Lys
370 375 380
att ctc ggt gct cag ttc tta agt gcg cat gaa gtg gca cag tct gct 1200
Ile Leu Gly Ala Gln Phe Leu Ser Ala His Glu Val Ala Gln Ser Ala
385 390 395 400
aat gcg tta tca atc gcg att caa aat gga aac acc atc gat gat cta 1248
Asn Ala Leu Ser Ile Ala Ile Gln Asn Gly Asn Thr Ile Asp Asp Leu
405 410 415
gca ttt gtg gat atg tta ttc tca cct aat ttt gat gaa cca ttc aat 1296
Ala Phe Val Asp Met Leu Phe Ser Pro Asn Phe Asp Glu Pro Phe Asn
420 425 430
tat ctg aac ctc gtc gcc caa caa gcg att gaa caa gaa cgc cag gca 1344
Tyr Leu Asn Leu Val Ala Gln Gln Ala Ile Glu Gln Glu Arg Gln Ala
435 440 445
ggc cgc aat cac cca cga att acg gcc tat ggc gat tgg gcc aag caa 1392
Gly Arg Asn His Pro Arg Ile Thr Ala Tyr Gly Asp Trp Ala Lys Gln
450 455 460
aat gac aag gat tta caa taa 1413
Asn Asp Lys Asp Leu Gln
465 470
<210> 120
<211> 470
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 120
Met Lys Ile Val Ile Val Gly Cys Thr His Ala Gly Thr Ala Ala Ala
1 5 10 15
Ala Gln Ile Leu Lys Asp His Pro Glu Ala Thr Val Thr Ile Tyr Glu
20 25 30
Arg Asp Asp Asn Ile Ser Phe Leu Ser Cys Gly Ile Tyr Leu Tyr Leu
35 40 45
Gly Gly Lys Val Asn His Leu Glu Asp Met Phe Tyr Ser Ser Pro Glu
50 55 60
Thr Leu Ala Lys Leu Gly Ala Met Val Arg Thr Lys His Asn Val Leu
65 70 75 80
Lys Ile Asp Ala Gln Ser Lys Thr Leu Gln Val Val Asp Met Thr Thr
85 90 95
Gly Lys Val Phe Gly Asp Thr Tyr Asp Lys Leu Ile Met Thr Thr Gly
100 105 110
Ser Asn Val Ala Val Pro Pro Ile Phe Gly Ile Asp Glu Ser Lys Val
115 120 125
Leu Leu Cys Lys Thr Tyr Ser Gln Ala Gln Glu Ile Tyr Gln Thr Ala
130 135 140
Gln Asp Asn Gln Arg Ile Ala Ile Ile Gly Ala Gly Tyr Ile Gly Thr
145 150 155 160
Glu Leu Ser Glu Ser Tyr Ala Asn Thr His His Glu Val Thr Leu Phe
165 170 175
Gln Ser His Asp Gln Ile Leu Asn Asn Tyr Ile Ser Lys Glu Ile Ser
180 185 190
Asp Gln Ala Val Gln Leu Leu Lys Asp His Gly Val Asn Val Leu Leu
195 200 205
Asn His Gln Val Thr Ala Phe Thr Gly Asn Asp Asp Gly Glu Leu Val
210 215 220
Ile Glu Thr Asn Gln Gly Asp Phe Asn Ala Asp Leu Ala Ile Val Gly
225 230 235 240
Thr Gly Phe Val Pro Asn Thr Glu Leu Leu Arg Gly Gln Val Glu Met
245 250 255
Asp Lys His Gly Ala Ile Ile Ile Asn Asp Tyr Val Gln Thr Ser Asp
260 265 270
Pro Asp Ile Tyr Ala Ala Gly Asp Ser Cys Val Val Asn Phe Asn Pro
275 280 285
Thr Gly Arg Ser Ala Tyr Thr Pro Leu Ala Thr Asn Ala Val Arg Gln
290 295 300
Gly Ala Leu Ala Gly Ala Asn Val Phe Gly Asn Gln Gln Arg Tyr Met
305 310 315 320
Gly Thr Gln Ala Thr Ser Ala Met Gln Leu Phe Asp His Thr Leu Ala
325 330 335
Thr Thr Gly Leu Thr Leu Ala Met Ala Lys Leu Asn His Ile Pro Ala
340 345 350
Glu Arg Val Thr Tyr Glu Gly Asn Trp Arg Pro Ala Tyr Met Pro Ser
355 360 365
Thr Ala Lys Leu Thr Ile Glu Leu Ile Tyr Asn Pro Glu Asn Arg Lys
370 375 380
Ile Leu Gly Ala Gln Phe Leu Ser Ala His Glu Val Ala Gln Ser Ala
385 390 395 400
Asn Ala Leu Ser Ile Ala Ile Gln Asn Gly Asn Thr Ile Asp Asp Leu
405 410 415
Ala Phe Val Asp Met Leu Phe Ser Pro Asn Phe Asp Glu Pro Phe Asn
420 425 430
Tyr Leu Asn Leu Val Ala Gln Gln Ala Ile Glu Gln Glu Arg Gln Ala
435 440 445
Gly Arg Asn His Pro Arg Ile Thr Ala Tyr Gly Asp Trp Ala Lys Gln
450 455 460
Asn Asp Lys Asp Leu Gln
465 470
<210> 121
<211> 1359
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(1359)
<223> 植物乳杆菌 WCFS1; 基因 lp_0766 编码形成H2O的NADH氧化酶
<400> 121
atg aaa gtt gcc att att ggc tgt aca cat gcg ggg att gcc gcc atg 48
Met Lys Val Ala Ile Ile Gly Cys Thr His Ala Gly Ile Ala Ala Met
1 5 10 15
aaa caa atc ttg aaa tat tat ccg ggc act gaa att acc gtc tat gaa 96
Lys Gln Ile Leu Lys Tyr Tyr Pro Gly Thr Glu Ile Thr Val Tyr Glu
20 25 30
cgg cac gcg gat att tca tat atg tct tgt ggg act tat cta cac tta 144
Arg His Ala Asp Ile Ser Tyr Met Ser Cys Gly Thr Tyr Leu His Leu
35 40 45
ggg ggt acc atc cat aat ttg gat caa gcg ttg tac gct aat cct agt 192
Gly Gly Thr Ile His Asn Leu Asp Gln Ala Leu Tyr Ala Asn Pro Ser
50 55 60
gaa ttt agt gaa caa ggt gtg cag atg cgg atg cag tat gat gtg atc 240
Glu Phe Ser Glu Gln Gly Val Gln Met Arg Met Gln Tyr Asp Val Ile
65 70 75 80
aat gtg aat gct gat aag cat aca att ttg gct caa aat tta cag aca 288
Asn Val Asn Ala Asp Lys His Thr Ile Leu Ala Gln Asn Leu Gln Thr
85 90 95
aaa gaa tta caa acg gat cac tat gat aag tta gtc atg gcg acg ggt 336
Lys Glu Leu Gln Thr Asp His Tyr Asp Lys Leu Val Met Ala Thr Gly
100 105 110
tca atc act gca atc ccg gct att ccg ggc gta gag aat cct aag gtc 384
Ser Ile Thr Ala Ile Pro Ala Ile Pro Gly Val Glu Asn Pro Lys Val
115 120 125
atg ctt tgc aaa aca tgt gaa cag gcc cag caa ctc tac gaa gca gct 432
Met Leu Cys Lys Thr Cys Glu Gln Ala Gln Gln Leu Tyr Glu Ala Ala
130 135 140
aaa cat gca cac cat att gca atc gtt ggt ggt ggc tat tcg ggg gtg 480
Lys His Ala His His Ile Ala Ile Val Gly Gly Gly Tyr Ser Gly Val
145 150 155 160
gaa ctt gct gag ggg tac gtg aag tct gga cac act gtg acg ttg ttt 528
Glu Leu Ala Glu Gly Tyr Val Lys Ser Gly His Thr Val Thr Leu Phe
165 170 175
caa cgg ggc ccc cac tta att aat gag tat ctt gat cca caa att tct 576
Gln Arg Gly Pro His Leu Ile Asn Glu Tyr Leu Asp Pro Gln Ile Ser
180 185 190
gag aag atc gaa gac ttg tta acg acg aaa ggc atc caa gtt aga acc 624
Glu Lys Ile Glu Asp Leu Leu Thr Thr Lys Gly Ile Gln Val Arg Thr
195 200 205
aat gca caa gtg acg gct ttt agt gat acg gct gat aat cga cta cga 672
Asn Ala Gln Val Thr Ala Phe Ser Asp Thr Ala Asp Asn Arg Leu Arg
210 215 220
gtt gca acc gct gat agt gaa gaa ata ttt gat atg gct gtt att tgt 720
Val Ala Thr Ala Asp Ser Glu Glu Ile Phe Asp Met Ala Val Ile Cys
225 230 235 240
ccg ggg gtg att cca caa tca gag tta ttg gcg gga cag gtc aac cga 768
Pro Gly Val Ile Pro Gln Ser Glu Leu Leu Ala Gly Gln Val Asn Arg
245 250 255
acg aag cag ggg gcc att gta acg gac cgt tat atg gga acc tcc aac 816
Thr Lys Gln Gly Ala Ile Val Thr Asp Arg Tyr Met Gly Thr Ser Asn
260 265 270
ccg gat ata ttt gcg gct ggc gat gtg acg gaa gtt aac ttt aat cct 864
Pro Asp Ile Phe Ala Ala Gly Asp Val Thr Glu Val Asn Phe Asn Pro
275 280 285
acg aag gaa cac gcc tat atg cca tta gtc tca cat gca att cgg caa 912
Thr Lys Glu His Ala Tyr Met Pro Leu Val Ser His Ala Ile Arg Gln
290 295 300
ggt gca ctg gca gga att aat atc ttt gat aaa cgc tta ggt gca att 960
Gly Ala Leu Ala Gly Ile Asn Ile Phe Asp Lys Arg Leu Gly Ala Ile
305 310 315 320
ggt acg caa gcg acg act ggg atg ttg att ttt gat cag acc gtg gct 1008
Gly Thr Gln Ala Thr Thr Gly Met Leu Ile Phe Asp Gln Thr Val Ala
325 330 335
tgt gca ggt ctg acc tta acg gcg gcc aaa gca gct aac ttc gat gcc 1056
Cys Ala Gly Leu Thr Leu Thr Ala Ala Lys Ala Ala Asn Phe Asp Ala
340 345 350
caa gca gct ttt tat gaa ggc aat tat cgg ccg gac ttt atg cct acc 1104
Gln Ala Ala Phe Tyr Glu Gly Asn Tyr Arg Pro Asp Phe Met Pro Thr
355 360 365
acg gcg aaa gtc atg att gag ttg gtt tat gat caa caa acg cgg aag 1152
Thr Ala Lys Val Met Ile Glu Leu Val Tyr Asp Gln Gln Thr Arg Lys
370 375 380
gtt ttg ggt ggt caa cta atg agt gct cat gaa gtt tca caa tca gca 1200
Val Leu Gly Gly Gln Leu Met Ser Ala His Glu Val Ser Gln Ser Ala
385 390 395 400
aat acg ttg tcc gta gtg att cat aat ggt aat acg att gat gaa tta 1248
Asn Thr Leu Ser Val Val Ile His Asn Gly Asn Thr Ile Asp Glu Leu
405 410 415
gcc ttc atg gat atg cta ttc tca ccc aac ttt gat gaa ccg ttt aat 1296
Ala Phe Met Asp Met Leu Phe Ser Pro Asn Phe Asp Glu Pro Phe Asn
420 425 430
tac ttg aat tta gtg gcg cag gct gca gta gat cag gaa cat ggc tat 1344
Tyr Leu Asn Leu Val Ala Gln Ala Ala Val Asp Gln Glu His Gly Tyr
435 440 445
cgt cgg aat aat taa 1359
Arg Arg Asn Asn
450
<210> 122
<211> 452
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 122
Met Lys Val Ala Ile Ile Gly Cys Thr His Ala Gly Ile Ala Ala Met
1 5 10 15
Lys Gln Ile Leu Lys Tyr Tyr Pro Gly Thr Glu Ile Thr Val Tyr Glu
20 25 30
Arg His Ala Asp Ile Ser Tyr Met Ser Cys Gly Thr Tyr Leu His Leu
35 40 45
Gly Gly Thr Ile His Asn Leu Asp Gln Ala Leu Tyr Ala Asn Pro Ser
50 55 60
Glu Phe Ser Glu Gln Gly Val Gln Met Arg Met Gln Tyr Asp Val Ile
65 70 75 80
Asn Val Asn Ala Asp Lys His Thr Ile Leu Ala Gln Asn Leu Gln Thr
85 90 95
Lys Glu Leu Gln Thr Asp His Tyr Asp Lys Leu Val Met Ala Thr Gly
100 105 110
Ser Ile Thr Ala Ile Pro Ala Ile Pro Gly Val Glu Asn Pro Lys Val
115 120 125
Met Leu Cys Lys Thr Cys Glu Gln Ala Gln Gln Leu Tyr Glu Ala Ala
130 135 140
Lys His Ala His His Ile Ala Ile Val Gly Gly Gly Tyr Ser Gly Val
145 150 155 160
Glu Leu Ala Glu Gly Tyr Val Lys Ser Gly His Thr Val Thr Leu Phe
165 170 175
Gln Arg Gly Pro His Leu Ile Asn Glu Tyr Leu Asp Pro Gln Ile Ser
180 185 190
Glu Lys Ile Glu Asp Leu Leu Thr Thr Lys Gly Ile Gln Val Arg Thr
195 200 205
Asn Ala Gln Val Thr Ala Phe Ser Asp Thr Ala Asp Asn Arg Leu Arg
210 215 220
Val Ala Thr Ala Asp Ser Glu Glu Ile Phe Asp Met Ala Val Ile Cys
225 230 235 240
Pro Gly Val Ile Pro Gln Ser Glu Leu Leu Ala Gly Gln Val Asn Arg
245 250 255
Thr Lys Gln Gly Ala Ile Val Thr Asp Arg Tyr Met Gly Thr Ser Asn
260 265 270
Pro Asp Ile Phe Ala Ala Gly Asp Val Thr Glu Val Asn Phe Asn Pro
275 280 285
Thr Lys Glu His Ala Tyr Met Pro Leu Val Ser His Ala Ile Arg Gln
290 295 300
Gly Ala Leu Ala Gly Ile Asn Ile Phe Asp Lys Arg Leu Gly Ala Ile
305 310 315 320
Gly Thr Gln Ala Thr Thr Gly Met Leu Ile Phe Asp Gln Thr Val Ala
325 330 335
Cys Ala Gly Leu Thr Leu Thr Ala Ala Lys Ala Ala Asn Phe Asp Ala
340 345 350
Gln Ala Ala Phe Tyr Glu Gly Asn Tyr Arg Pro Asp Phe Met Pro Thr
355 360 365
Thr Ala Lys Val Met Ile Glu Leu Val Tyr Asp Gln Gln Thr Arg Lys
370 375 380
Val Leu Gly Gly Gln Leu Met Ser Ala His Glu Val Ser Gln Ser Ala
385 390 395 400
Asn Thr Leu Ser Val Val Ile His Asn Gly Asn Thr Ile Asp Glu Leu
405 410 415
Ala Phe Met Asp Met Leu Phe Ser Pro Asn Phe Asp Glu Pro Phe Asn
420 425 430
Tyr Leu Asn Leu Val Ala Gln Ala Ala Val Asp Gln Glu His Gly Tyr
435 440 445
Arg Arg Asn Asn
450
<210> 123
<211> 1692
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(1692)
<223> 植物乳杆菌 WCFS1; 基因 lp_1925 编码形成H2O的NADH氧化酶
<400> 123
atg gaa gtg aaa aaa gtg gtc att gtt ggt ggc gtt ggt ggt ggt gct 48
Met Glu Val Lys Lys Val Val Ile Val Gly Gly Val Gly Gly Gly Ala
1 5 10 15
tcg gcg gcg gcc cgc gtg cgt cga ttg gac gag ttc gcc cag att aca 96
Ser Ala Ala Ala Arg Val Arg Arg Leu Asp Glu Phe Ala Gln Ile Thr
20 25 30
att ttt gaa aag ggg ccg gat gtc tca ttt tcc aat tgt gcc tta ccg 144
Ile Phe Glu Lys Gly Pro Asp Val Ser Phe Ser Asn Cys Ala Leu Pro
35 40 45
tac aca ttc agt gat gtg att gac cag ccc gat gac gtg att tta aag 192
Tyr Thr Phe Ser Asp Val Ile Asp Gln Pro Asp Asp Val Ile Leu Lys
50 55 60
aca ccg atg caa ttc gag caa cag tac aat att gac gtt caa gtg aat 240
Thr Pro Met Gln Phe Glu Gln Gln Tyr Asn Ile Asp Val Gln Val Asn
65 70 75 80
aca acg gtt gtc gcc att gat gcg cag gct aag aca gtg atg atc aaa 288
Thr Thr Val Val Ala Ile Asp Ala Gln Ala Lys Thr Val Met Ile Lys
85 90 95
cgg caa gga cag gta gca acg acg atc gtc cca tat gat aca ctg att 336
Arg Gln Gly Gln Val Ala Thr Thr Ile Val Pro Tyr Asp Thr Leu Ile
100 105 110
ctt tca cct ggt gct cga cca ata cta ccc gca gtg att gcg gga atc 384
Leu Ser Pro Gly Ala Arg Pro Ile Leu Pro Ala Val Ile Ala Gly Ile
115 120 125
gac cgg ccc aat gtt aaa acg gtc cgc aat gtt caa gat gtc cga acg 432
Asp Arg Pro Asn Val Lys Thr Val Arg Asn Val Gln Asp Val Arg Thr
130 135 140
att caa gcg tat ctg act gaa caa cac gtt cat gat gtg acg att gtt 480
Ile Gln Ala Tyr Leu Thr Glu Gln His Val His Asp Val Thr Ile Val
145 150 155 160
ggc ggt ggc ttt atc gga cta gag atg gct gaa aat tta caa cag gct 528
Gly Gly Gly Phe Ile Gly Leu Glu Met Ala Glu Asn Leu Gln Gln Ala
165 170 175
ggc tat cag atc aca atc att gag gct gcc gag cac gtc ttg ggc act 576
Gly Tyr Gln Ile Thr Ile Ile Glu Ala Ala Glu His Val Leu Gly Thr
180 185 190
ttg gat gac gag ttt gct cag cta gta cac aag gaa cta tac gat cac 624
Leu Asp Asp Glu Phe Ala Gln Leu Val His Lys Glu Leu Tyr Asp His
195 200 205
ggt gtg cag gtg ata ctg caa gaa caa gtg acg gcc att ggt gca aca 672
Gly Val Gln Val Ile Leu Gln Glu Gln Val Thr Ala Ile Gly Ala Thr
210 215 220
caa gtg cag ctc gcc tcg gga cgc gcc gtg acc agc cag tta gtg att 720
Gln Val Gln Leu Ala Ser Gly Arg Ala Val Thr Ser Gln Leu Val Ile
225 230 235 240
gtg gca att ggg gtt aca cca gta act gaa tta gcg gtt caa gcg ggt 768
Val Ala Ile Gly Val Thr Pro Val Thr Glu Leu Ala Val Gln Ala Gly
245 250 255
tgt gcg tta ggc gtg acc ggt ggt atc aag gtt gat cat cgt tac cag 816
Cys Ala Leu Gly Val Thr Gly Gly Ile Lys Val Asp His Arg Tyr Gln
260 265 270
acg tcc gtg gcc gac att tat gcg gtg ggt gat gcc att gaa gtg act 864
Thr Ser Val Ala Asp Ile Tyr Ala Val Gly Asp Ala Ile Glu Val Thr
275 280 285
aat cgg ctt aca cgt caa tct acg cgg cta gca ttg gca ttt ccc gca 912
Asn Arg Leu Thr Arg Gln Ser Thr Arg Leu Ala Leu Ala Phe Pro Ala
290 295 300
caa atg cag gcc cga cac atg gtg gat cat tta tac ggg cgg acg cca 960
Gln Met Gln Ala Arg His Met Val Asp His Leu Tyr Gly Arg Thr Pro
305 310 315 320
cag tat cag ggt gtg atc gga tcg cag gtt ctt cac gtc ttt acg tta 1008
Gln Tyr Gln Gly Val Ile Gly Ser Gln Val Leu His Val Phe Thr Leu
325 330 335
aac gtg gcg tca acg ggc ctg aac gct act gag tgt gag cac cag caa 1056
Asn Val Ala Ser Thr Gly Leu Asn Ala Thr Glu Cys Glu His Gln Gln
340 345 350
ctt gat tat cgg ttc gca acg gtg gca cca cag aat cgg gtc agc gtg 1104
Leu Asp Tyr Arg Phe Ala Thr Val Ala Pro Gln Asn Arg Val Ser Val
355 360 365
atg cca ggg aca aca gcc gtc ttt ctt aag ttg ata ttc agt tat ccg 1152
Met Pro Gly Thr Thr Ala Val Phe Leu Lys Leu Ile Phe Ser Tyr Pro
370 375 380
acc ggt gag att ttg ggc gcg cag gcc att ggt caa tcg gac gtt gac 1200
Thr Gly Glu Ile Leu Gly Ala Gln Ala Ile Gly Gln Ser Asp Val Asp
385 390 395 400
aaa ccg att gat att atc gct agt cta atc acg atg cac ggt cga gtc 1248
Lys Pro Ile Asp Ile Ile Ala Ser Leu Ile Thr Met His Gly Arg Val
405 410 415
agt gac ctg aca gat gtt gaa tta tgt tat tcg cca tgg gtc agt acc 1296
Ser Asp Leu Thr Asp Val Glu Leu Cys Tyr Ser Pro Trp Val Ser Thr
420 425 430
gcc aaa aat gtc gtc aac atg gtt ggc ttg gtg gcg gaa aac gtc ctg 1344
Ala Lys Asn Val Val Asn Met Val Gly Leu Val Ala Glu Asn Val Leu
435 440 445
aat gat gaa ttt aag cag gtt gcg gtg aca cag gtt cga cag ctg gtc 1392
Asn Asp Glu Phe Lys Gln Val Ala Val Thr Gln Val Arg Gln Leu Val
450 455 460
ttg gcg ggg gcg ttt atc atc gat gcc cgt gaa cca caa gag tac gct 1440
Leu Ala Gly Ala Phe Ile Ile Asp Ala Arg Glu Pro Gln Glu Tyr Ala
465 470 475 480
gat ggg cac atc aag ggc gcg cat aat att cca ctg agt cag ttt cga 1488
Asp Gly His Ile Lys Gly Ala His Asn Ile Pro Leu Ser Gln Phe Arg
485 490 495
cag cgc tta gtg gaa att cca acc aat cag cca gtt tat att cat tgt 1536
Gln Arg Leu Val Glu Ile Pro Thr Asn Gln Pro Val Tyr Ile His Cys
500 505 510
cag acc gga cag cga agt tac aat atg gtc cgc gcg tta ctt cag cgc 1584
Gln Thr Gly Gln Arg Ser Tyr Asn Met Val Arg Ala Leu Leu Gln Arg
515 520 525
ggc tat acg aat gtg gtc aac att gct ggg tcg tat gcc gaa cta gtt 1632
Gly Tyr Thr Asn Val Val Asn Ile Ala Gly Ser Tyr Ala Glu Leu Val
530 535 540
cag tat gaa tat ttt aat gat cag aca aca ggg cga acc tca att ctg 1680
Gln Tyr Glu Tyr Phe Asn Asp Gln Thr Thr Gly Arg Thr Ser Ile Leu
545 550 555 560
aca cga aca tga 1692
Thr Arg Thr
<210> 124
<211> 563
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 124
Met Glu Val Lys Lys Val Val Ile Val Gly Gly Val Gly Gly Gly Ala
1 5 10 15
Ser Ala Ala Ala Arg Val Arg Arg Leu Asp Glu Phe Ala Gln Ile Thr
20 25 30
Ile Phe Glu Lys Gly Pro Asp Val Ser Phe Ser Asn Cys Ala Leu Pro
35 40 45
Tyr Thr Phe Ser Asp Val Ile Asp Gln Pro Asp Asp Val Ile Leu Lys
50 55 60
Thr Pro Met Gln Phe Glu Gln Gln Tyr Asn Ile Asp Val Gln Val Asn
65 70 75 80
Thr Thr Val Val Ala Ile Asp Ala Gln Ala Lys Thr Val Met Ile Lys
85 90 95
Arg Gln Gly Gln Val Ala Thr Thr Ile Val Pro Tyr Asp Thr Leu Ile
100 105 110
Leu Ser Pro Gly Ala Arg Pro Ile Leu Pro Ala Val Ile Ala Gly Ile
115 120 125
Asp Arg Pro Asn Val Lys Thr Val Arg Asn Val Gln Asp Val Arg Thr
130 135 140
Ile Gln Ala Tyr Leu Thr Glu Gln His Val His Asp Val Thr Ile Val
145 150 155 160
Gly Gly Gly Phe Ile Gly Leu Glu Met Ala Glu Asn Leu Gln Gln Ala
165 170 175
Gly Tyr Gln Ile Thr Ile Ile Glu Ala Ala Glu His Val Leu Gly Thr
180 185 190
Leu Asp Asp Glu Phe Ala Gln Leu Val His Lys Glu Leu Tyr Asp His
195 200 205
Gly Val Gln Val Ile Leu Gln Glu Gln Val Thr Ala Ile Gly Ala Thr
210 215 220
Gln Val Gln Leu Ala Ser Gly Arg Ala Val Thr Ser Gln Leu Val Ile
225 230 235 240
Val Ala Ile Gly Val Thr Pro Val Thr Glu Leu Ala Val Gln Ala Gly
245 250 255
Cys Ala Leu Gly Val Thr Gly Gly Ile Lys Val Asp His Arg Tyr Gln
260 265 270
Thr Ser Val Ala Asp Ile Tyr Ala Val Gly Asp Ala Ile Glu Val Thr
275 280 285
Asn Arg Leu Thr Arg Gln Ser Thr Arg Leu Ala Leu Ala Phe Pro Ala
290 295 300
Gln Met Gln Ala Arg His Met Val Asp His Leu Tyr Gly Arg Thr Pro
305 310 315 320
Gln Tyr Gln Gly Val Ile Gly Ser Gln Val Leu His Val Phe Thr Leu
325 330 335
Asn Val Ala Ser Thr Gly Leu Asn Ala Thr Glu Cys Glu His Gln Gln
340 345 350
Leu Asp Tyr Arg Phe Ala Thr Val Ala Pro Gln Asn Arg Val Ser Val
355 360 365
Met Pro Gly Thr Thr Ala Val Phe Leu Lys Leu Ile Phe Ser Tyr Pro
370 375 380
Thr Gly Glu Ile Leu Gly Ala Gln Ala Ile Gly Gln Ser Asp Val Asp
385 390 395 400
Lys Pro Ile Asp Ile Ile Ala Ser Leu Ile Thr Met His Gly Arg Val
405 410 415
Ser Asp Leu Thr Asp Val Glu Leu Cys Tyr Ser Pro Trp Val Ser Thr
420 425 430
Ala Lys Asn Val Val Asn Met Val Gly Leu Val Ala Glu Asn Val Leu
435 440 445
Asn Asp Glu Phe Lys Gln Val Ala Val Thr Gln Val Arg Gln Leu Val
450 455 460
Leu Ala Gly Ala Phe Ile Ile Asp Ala Arg Glu Pro Gln Glu Tyr Ala
465 470 475 480
Asp Gly His Ile Lys Gly Ala His Asn Ile Pro Leu Ser Gln Phe Arg
485 490 495
Gln Arg Leu Val Glu Ile Pro Thr Asn Gln Pro Val Tyr Ile His Cys
500 505 510
Gln Thr Gly Gln Arg Ser Tyr Asn Met Val Arg Ala Leu Leu Gln Arg
515 520 525
Gly Tyr Thr Asn Val Val Asn Ile Ala Gly Ser Tyr Ala Glu Leu Val
530 535 540
Gln Tyr Glu Tyr Phe Asn Asp Gln Thr Thr Gly Arg Thr Ser Ile Leu
545 550 555 560
Thr Arg Thr
<210> 125
<211> 1407
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(1407)
<223> 植物乳杆菌 WCFS1; 基因 lp_1941 编码形成H2O的NADH氧化酶
<400> 125
atg aaa gta atg atc atc ggt agt acc cac gct ggt acg gca gcg gca 48
Met Lys Val Met Ile Ile Gly Ser Thr His Ala Gly Thr Ala Ala Ala
1 5 10 15
cgc gaa att tta aca cgt cat ccg gag acg gcg gtg acg att tac gag 96
Arg Glu Ile Leu Thr Arg His Pro Glu Thr Ala Val Thr Ile Tyr Glu
20 25 30
cgg aat gat aac gtt tcg ttt gtt tcg agt ggg att tat ttg tac tta 144
Arg Asn Asp Asn Val Ser Phe Val Ser Ser Gly Ile Tyr Leu Tyr Leu
35 40 45
acg ggt gtc atc caa cac ctg gaa gac atg ttt tac acc tcg ccg tct 192
Thr Gly Val Ile Gln His Leu Glu Asp Met Phe Tyr Thr Ser Pro Ser
50 55 60
gac ttg aag cag tta ggc gct caa gtt aag acc cgc cat aat gtt tta 240
Asp Leu Lys Gln Leu Gly Ala Gln Val Lys Thr Arg His Asn Val Leu
65 70 75 80
cga att gat acg ggc cat aaa acg gtg cag gtc gcc aat atg cag act 288
Arg Ile Asp Thr Gly His Lys Thr Val Gln Val Ala Asn Met Gln Thr
85 90 95
ggc gaa gtc ttt gat gac act tat gat aag ctg att atg gca act ggt 336
Gly Glu Val Phe Asp Asp Thr Tyr Asp Lys Leu Ile Met Ala Thr Gly
100 105 110
tca tca gtt att gtg cca cca att atg ggt atc gat cat gag aaa gta 384
Ser Ser Val Ile Val Pro Pro Ile Met Gly Ile Asp His Glu Lys Val
115 120 125
tta ctg tgc aaa aac tac gag cag gcg gaa caa ctt tat caa tct cta 432
Leu Leu Cys Lys Asn Tyr Glu Gln Ala Glu Gln Leu Tyr Gln Ser Leu
130 135 140
cag gcc aac caa cgc gtt gca att gtt ggg gcc gga tat atg ggg acc 480
Gln Ala Asn Gln Arg Val Ala Ile Val Gly Ala Gly Tyr Met Gly Thr
145 150 155 160
gag tta gcg gaa agc tat gct agt atg gac caa caa gtc atg tta ttt 528
Glu Leu Ala Glu Ser Tyr Ala Ser Met Asp Gln Gln Val Met Leu Phe
165 170 175
cat tcg cat tca cat att ttg aat aat tat ctt ggc ccc caa atg gcg 576
His Ser His Ser His Ile Leu Asn Asn Tyr Leu Gly Pro Gln Met Ala
180 185 190
gat gct gca act aaa ctc tta caa cat cac cag gtc gat atc cac ctc 624
Asp Ala Ala Thr Lys Leu Leu Gln His His Gln Val Asp Ile His Leu
195 200 205
aat gaa cgg gtg act gga ttc gca agc ggt tcg aat gat cag ttg atc 672
Asn Glu Arg Val Thr Gly Phe Ala Ser Gly Ser Asn Asp Gln Leu Ile
210 215 220
gtt gaa acc gcg cag ggt gat tat gag gtt gat ctg gcc gtc gtt tgc 720
Val Glu Thr Ala Gln Gly Asp Tyr Glu Val Asp Leu Ala Val Val Cys
225 230 235 240
gcg ggc ttt atg ccg aat acg gaa ttg tta cgc ggg cag gtc gct atg 768
Ala Gly Phe Met Pro Asn Thr Glu Leu Leu Arg Gly Gln Val Ala Met
245 250 255
gac cgc cat ggt gct atc ttg atc aat gat tat gtt caa acc tct gac 816
Asp Arg His Gly Ala Ile Leu Ile Asn Asp Tyr Val Gln Thr Ser Asp
260 265 270
ccg gat att tat gcg gct ggg gat gct tgc gtg gtc aat tat aat ccc 864
Pro Asp Ile Tyr Ala Ala Gly Asp Ala Cys Val Val Asn Tyr Asn Pro
275 280 285
acg gga cag ccg gcg tat aca cca ttg gca act aac gca ctg cgc caa 912
Thr Gly Gln Pro Ala Tyr Thr Pro Leu Ala Thr Asn Ala Leu Arg Gln
290 295 300
ggt gcg ttg gcg ggg atc aat gtt ttc gga gat tta cag ccg tac atg 960
Gly Ala Leu Ala Gly Ile Asn Val Phe Gly Asp Leu Gln Pro Tyr Met
305 310 315 320
gga acg caa gcc acc tca gcc atg caa tta ttt gat cac acg atg gcc 1008
Gly Thr Gln Ala Thr Ser Ala Met Gln Leu Phe Asp His Thr Met Ala
325 330 335
aca act ggg ttg acc tat cta gcc gcg caa aga cga gaa cta cca gta 1056
Thr Thr Gly Leu Thr Tyr Leu Ala Ala Gln Arg Arg Glu Leu Pro Val
340 345 350
cga cac gcc gtc tat caa ggc aat tgg cgg cca gct tat atg ccg acg 1104
Arg His Ala Val Tyr Gln Gly Asn Trp Arg Pro Ala Tyr Met Pro Thr
355 360 365
acg gcc aaa gtc acg att gaa cta gtt tat aat tat cag aat cgt cag 1152
Thr Ala Lys Val Thr Ile Glu Leu Val Tyr Asn Tyr Gln Asn Arg Gln
370 375 380
ctt ctc ggc gcg caa ttc tgg agt cgt cac gac att gcc caa gct gca 1200
Leu Leu Gly Ala Gln Phe Trp Ser Arg His Asp Ile Ala Gln Ala Ala
385 390 395 400
aac acg atc tca gta atg att caa aat cag aat acg att gat gat ttg 1248
Asn Thr Ile Ser Val Met Ile Gln Asn Gln Asn Thr Ile Asp Asp Leu
405 410 415
gct tac gtg gat atg ttc ttt cag ccg aat ttt aat caa ccg ttc aac 1296
Ala Tyr Val Asp Met Phe Phe Gln Pro Asn Phe Asn Gln Pro Phe Asn
420 425 430
tat tta aat ttg gtg ggg caa cta gcc gtc aaa caa gaa tat cag cga 1344
Tyr Leu Asn Leu Val Gly Gln Leu Ala Val Lys Gln Glu Tyr Gln Arg
435 440 445
ggc aat caa aca cca cga att aca gct cgt ggt aac tgg tcg act agt 1392
Gly Asn Gln Thr Pro Arg Ile Thr Ala Arg Gly Asn Trp Ser Thr Ser
450 455 460
agt gat aag cat tga 1407
Ser Asp Lys His
465
<210> 126
<211> 468
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 126
Met Lys Val Met Ile Ile Gly Ser Thr His Ala Gly Thr Ala Ala Ala
1 5 10 15
Arg Glu Ile Leu Thr Arg His Pro Glu Thr Ala Val Thr Ile Tyr Glu
20 25 30
Arg Asn Asp Asn Val Ser Phe Val Ser Ser Gly Ile Tyr Leu Tyr Leu
35 40 45
Thr Gly Val Ile Gln His Leu Glu Asp Met Phe Tyr Thr Ser Pro Ser
50 55 60
Asp Leu Lys Gln Leu Gly Ala Gln Val Lys Thr Arg His Asn Val Leu
65 70 75 80
Arg Ile Asp Thr Gly His Lys Thr Val Gln Val Ala Asn Met Gln Thr
85 90 95
Gly Glu Val Phe Asp Asp Thr Tyr Asp Lys Leu Ile Met Ala Thr Gly
100 105 110
Ser Ser Val Ile Val Pro Pro Ile Met Gly Ile Asp His Glu Lys Val
115 120 125
Leu Leu Cys Lys Asn Tyr Glu Gln Ala Glu Gln Leu Tyr Gln Ser Leu
130 135 140
Gln Ala Asn Gln Arg Val Ala Ile Val Gly Ala Gly Tyr Met Gly Thr
145 150 155 160
Glu Leu Ala Glu Ser Tyr Ala Ser Met Asp Gln Gln Val Met Leu Phe
165 170 175
His Ser His Ser His Ile Leu Asn Asn Tyr Leu Gly Pro Gln Met Ala
180 185 190
Asp Ala Ala Thr Lys Leu Leu Gln His His Gln Val Asp Ile His Leu
195 200 205
Asn Glu Arg Val Thr Gly Phe Ala Ser Gly Ser Asn Asp Gln Leu Ile
210 215 220
Val Glu Thr Ala Gln Gly Asp Tyr Glu Val Asp Leu Ala Val Val Cys
225 230 235 240
Ala Gly Phe Met Pro Asn Thr Glu Leu Leu Arg Gly Gln Val Ala Met
245 250 255
Asp Arg His Gly Ala Ile Leu Ile Asn Asp Tyr Val Gln Thr Ser Asp
260 265 270
Pro Asp Ile Tyr Ala Ala Gly Asp Ala Cys Val Val Asn Tyr Asn Pro
275 280 285
Thr Gly Gln Pro Ala Tyr Thr Pro Leu Ala Thr Asn Ala Leu Arg Gln
290 295 300
Gly Ala Leu Ala Gly Ile Asn Val Phe Gly Asp Leu Gln Pro Tyr Met
305 310 315 320
Gly Thr Gln Ala Thr Ser Ala Met Gln Leu Phe Asp His Thr Met Ala
325 330 335
Thr Thr Gly Leu Thr Tyr Leu Ala Ala Gln Arg Arg Glu Leu Pro Val
340 345 350
Arg His Ala Val Tyr Gln Gly Asn Trp Arg Pro Ala Tyr Met Pro Thr
355 360 365
Thr Ala Lys Val Thr Ile Glu Leu Val Tyr Asn Tyr Gln Asn Arg Gln
370 375 380
Leu Leu Gly Ala Gln Phe Trp Ser Arg His Asp Ile Ala Gln Ala Ala
385 390 395 400
Asn Thr Ile Ser Val Met Ile Gln Asn Gln Asn Thr Ile Asp Asp Leu
405 410 415
Ala Tyr Val Asp Met Phe Phe Gln Pro Asn Phe Asn Gln Pro Phe Asn
420 425 430
Tyr Leu Asn Leu Val Gly Gln Leu Ala Val Lys Gln Glu Tyr Gln Arg
435 440 445
Gly Asn Gln Thr Pro Arg Ile Thr Ala Arg Gly Asn Trp Ser Thr Ser
450 455 460
Ser Asp Lys His
465
<210> 127
<211> 1353
<212> DNA
<213> 植物乳杆菌（Lactobacillus plantarum）
<220>
<221> CDS
<222> (1)..(1353)
<223> 植物乳杆菌 WCFS1; 基因 lp_3449 编码形成H2O的NADH氧化酶
<400> 127
atg aaa gtt att gta att ggt tgt acc cat gcc ggc act gct gcc gtt 48
Met Lys Val Ile Val Ile Gly Cys Thr His Ala Gly Thr Ala Ala Val
1 5 10 15
aat cag att tta gca tca aat cca gat act gaa gtg acg att tat gaa 96
Asn Gln Ile Leu Ala Ser Asn Pro Asp Thr Glu Val Thr Ile Tyr Glu
20 25 30
aga aat gac aat gtc tcg ttc cta tcc tgt ggg atc gca ctt tac ctt 144
Arg Asn Asp Asn Val Ser Phe Leu Ser Cys Gly Ile Ala Leu Tyr Leu
35 40 45
ggc ggc caa gtt gct gat cct caa ggc cta ttt tat tcc agt cct gaa 192
Gly Gly Gln Val Ala Asp Pro Gln Gly Leu Phe Tyr Ser Ser Pro Glu
50 55 60
cag tta gct aag tta ggc gca act gtt cat atg caa cat gat gtg acg 240
Gln Leu Ala Lys Leu Gly Ala Thr Val His Met Gln His Asp Val Thr
65 70 75 80
gat gtg aat act gac aaa cat gaa att acg gtt act gat tta aag act 288
Asp Val Asn Thr Asp Lys His Glu Ile Thr Val Thr Asp Leu Lys Thr
85 90 95
ggt gaa tct aag act gat cac tat gac aag tta gtt gtc act act ggt 336
Gly Glu Ser Lys Thr Asp His Tyr Asp Lys Leu Val Val Thr Thr Gly
100 105 110
tca tgg cct gtt att cca cca att gac ggc atc gat agt ccc aat gtc 384
Ser Trp Pro Val Ile Pro Pro Ile Asp Gly Ile Asp Ser Pro Asn Val
115 120 125
tac tta tgc aag aac tgg acg cac gct cag aat tta tgg gaa gca gcc 432
Tyr Leu Cys Lys Asn Trp Thr His Ala Gln Asn Leu Trp Glu Ala Ala
130 135 140
aaa cca gct aag cgg gtc att gtg atc ggt ggc ggt tat atc ggt act 480
Lys Pro Ala Lys Arg Val Ile Val Ile Gly Gly Gly Tyr Ile Gly Thr
145 150 155 160
gaa tta gtt gaa gct tac cag aag caa ggt aag gaa gtc aca cta att 528
Glu Leu Val Glu Ala Tyr Gln Lys Gln Gly Lys Glu Val Thr Leu Ile
165 170 175
gat ggt tta cca cgg att tta aat aaa tac tta gac aaa gaa ttc act 576
Asp Gly Leu Pro Arg Ile Leu Asn Lys Tyr Leu Asp Lys Glu Phe Thr
180 185 190
gac cgg gtt gaa caa gac ttt gtt gat cac ggt atc aag atg gct ttg 624
Asp Arg Val Glu Gln Asp Phe Val Asp His Gly Ile Lys Met Ala Leu
195 200 205
aat caa atg gtg caa ggc ttc agt gat gat ggt aaa gaa gtt acg gtc 672
Asn Gln Met Val Gln Gly Phe Ser Asp Asp Gly Lys Glu Val Thr Val
210 215 220
aag act gac aag ggc agc tac aca gcc gac atg gcg att ctt tgt gtt 720
Lys Thr Asp Lys Gly Ser Tyr Thr Ala Asp Met Ala Ile Leu Cys Val
225 230 235 240
ggc ttc cgg cca aat acc ggc tta ctc aag ggc aag gtc gat atg aac 768
Gly Phe Arg Pro Asn Thr Gly Leu Leu Lys Gly Lys Val Asp Met Asn
245 250 255
gct aat ggc tcg atc aag acc aat gac tac atg caa act tct gat cca 816
Ala Asn Gly Ser Ile Lys Thr Asn Asp Tyr Met Gln Thr Ser Asp Pro
260 265 270
gac att tac ggg gct ggt gac tcg gtt gct gtt cac tat aac cca act 864
Asp Ile Tyr Gly Ala Gly Asp Ser Val Ala Val His Tyr Asn Pro Thr
275 280 285
aag aaa gat gct tat atc cca tta gca acg aat gcg gtt cgc caa gga 912
Lys Lys Asp Ala Tyr Ile Pro Leu Ala Thr Asn Ala Val Arg Gln Gly
290 295 300
acc tta gtt ggt ttg aac atc ttc aag cca acg cgg aag tac atg ggg 960
Thr Leu Val Gly Leu Asn Ile Phe Lys Pro Thr Arg Lys Tyr Met Gly
305 310 315 320
aca caa tca act tct ggg tta atg ttg ttc ggc caa acc atc gtt tca 1008
Thr Gln Ser Thr Ser Gly Leu Met Leu Phe Gly Gln Thr Ile Val Ser
325 330 335
tct ggg atg acc cta gaa cat gca cag gcc gaa aat gtt ccg gca gcc 1056
Ser Gly Met Thr Leu Glu His Ala Gln Ala Glu Asn Val Pro Ala Ala
340 345 350
gct gtc act ttt gaa gac aac tac cgg cca gaa ttt atg cca acc act 1104
Ala Val Thr Phe Glu Asp Asn Tyr Arg Pro Glu Phe Met Pro Thr Thr
355 360 365
aag cct gtt tta atg caa ttg gtt tac aat cct gaa act cgc gaa atc 1152
Lys Pro Val Leu Met Gln Leu Val Tyr Asn Pro Glu Thr Arg Glu Ile
370 375 380
cta ggt gca caa ttc atg agt gaa cat gat gtt tcg caa tca gcc aat 1200
Leu Gly Ala Gln Phe Met Ser Glu His Asp Val Ser Gln Ser Ala Asn
385 390 395 400
gtg att tca gtg atg att caa aat cac aat acc att gac gac tta ggg 1248
Val Ile Ser Val Met Ile Gln Asn His Asn Thr Ile Asp Asp Leu Gly
405 410 415
ttc gta gat atg ttc ttc caa cca att tac gac cgg cca ttt aac tat 1296
Phe Val Asp Met Phe Phe Gln Pro Ile Tyr Asp Arg Pro Phe Asn Tyr
420 425 430
ttg aac tta ctt ggt caa gca gcg att gcc cat gcg gcc gaa gct gtc 1344
Leu Asn Leu Leu Gly Gln Ala Ala Ile Ala His Ala Ala Glu Ala Val
435 440 445
act gaa taa 1353
Thr Glu
450
<210> 128
<211> 450
<212> PRT
<213> 植物乳杆菌（Lactobacillus plantarum）
<400> 128
Met Lys Val Ile Val Ile Gly Cys Thr His Ala Gly Thr Ala Ala Val
1 5 10 15
Asn Gln Ile Leu Ala Ser Asn Pro Asp Thr Glu Val Thr Ile Tyr Glu
20 25 30
Arg Asn Asp Asn Val Ser Phe Leu Ser Cys Gly Ile Ala Leu Tyr Leu
35 40 45
Gly Gly Gln Val Ala Asp Pro Gln Gly Leu Phe Tyr Ser Ser Pro Glu
50 55 60
Gln Leu Ala Lys Leu Gly Ala Thr Val His Met Gln His Asp Val Thr
65 70 75 80
Asp Val Asn Thr Asp Lys His Glu Ile Thr Val Thr Asp Leu Lys Thr
85 90 95
Gly Glu Ser Lys Thr Asp His Tyr Asp Lys Leu Val Val Thr Thr Gly
100 105 110
Ser Trp Pro Val Ile Pro Pro Ile Asp Gly Ile Asp Ser Pro Asn Val
115 120 125
Tyr Leu Cys Lys Asn Trp Thr His Ala Gln Asn Leu Trp Glu Ala Ala
130 135 140
Lys Pro Ala Lys Arg Val Ile Val Ile Gly Gly Gly Tyr Ile Gly Thr
145 150 155 160
Glu Leu Val Glu Ala Tyr Gln Lys Gln Gly Lys Glu Val Thr Leu Ile
165 170 175
Asp Gly Leu Pro Arg Ile Leu Asn Lys Tyr Leu Asp Lys Glu Phe Thr
180 185 190
Asp Arg Val Glu Gln Asp Phe Val Asp His Gly Ile Lys Met Ala Leu
195 200 205
Asn Gln Met Val Gln Gly Phe Ser Asp Asp Gly Lys Glu Val Thr Val
210 215 220
Lys Thr Asp Lys Gly Ser Tyr Thr Ala Asp Met Ala Ile Leu Cys Val
225 230 235 240
Gly Phe Arg Pro Asn Thr Gly Leu Leu Lys Gly Lys Val Asp Met Asn
245 250 255
Ala Asn Gly Ser Ile Lys Thr Asn Asp Tyr Met Gln Thr Ser Asp Pro
260 265 270
Asp Ile Tyr Gly Ala Gly Asp Ser Val Ala Val His Tyr Asn Pro Thr
275 280 285
Lys Lys Asp Ala Tyr Ile Pro Leu Ala Thr Asn Ala Val Arg Gln Gly
290 295 300
Thr Leu Val Gly Leu Asn Ile Phe Lys Pro Thr Arg Lys Tyr Met Gly
305 310 315 320
Thr Gln Ser Thr Ser Gly Leu Met Leu Phe Gly Gln Thr Ile Val Ser
325 330 335
Ser Gly Met Thr Leu Glu His Ala Gln Ala Glu Asn Val Pro Ala Ala
340 345 350
Ala Val Thr Phe Glu Asp Asn Tyr Arg Pro Glu Phe Met Pro Thr Thr
355 360 365
Lys Pro Val Leu Met Gln Leu Val Tyr Asn Pro Glu Thr Arg Glu Ile
370 375 380
Leu Gly Ala Gln Phe Met Ser Glu His Asp Val Ser Gln Ser Ala Asn
385 390 395 400
Val Ile Ser Val Met Ile Gln Asn His Asn Thr Ile Asp Asp Leu Gly
405 410 415
Phe Val Asp Met Phe Phe Gln Pro Ile Tyr Asp Arg Pro Phe Asn Tyr
420 425 430
Leu Asn Leu Leu Gly Gln Ala Ala Ile Ala His Ala Ala Glu Ala Val
435 440 445
Thr Glu
450
<210> 129
<211> 1386
<212> DNA
<213> 嗜热链球菌
<220>
<221> CDS
<222> (1)..(1386)
<223> 嗜热链球菌 CNRZ1066; 基因 str1281 编码形成H2O的NADH氧化酶
<220>
<221> misc_feature（新的或少见的特征）
<222> (1)..(3)
<223> 由起始密码子（TTG）处的tRNA插入Met而不是Leu
<400> 129
ttg gat aaa ttt atg tca aaa atc gta gtt gtc ggt gct aac cat gct 48
Leu Asp Lys Phe Met Ser Lys Ile Val Val Val Gly Ala Asn His Ala
1 5 10 15
ggt act tat agt att aat act att ctt gat aat tac gga gac caa aat 96
Gly Thr Tyr Ser Ile Asn Thr Ile Leu Asp Asn Tyr Gly Asp Gln Asn
20 25 30
gaa gta gtt gtt ttc gat caa aac tct aac att tca ttc ttg ggt tgt 144
Glu Val Val Val Phe Asp Gln Asn Ser Asn Ile Ser Phe Leu Gly Cys
35 40 45
ggt atg gct ctt tgg att ggt aat caa atc tca ggt cct gat ggc ctt 192
Gly Met Ala Leu Trp Ile Gly Asn Gln Ile Ser Gly Pro Asp Gly Leu
50 55 60
ttc tat gcc aac aaa gaa ggc ctt gaa agc aag ggt gca aaa gtt tat 240
Phe Tyr Ala Asn Lys Glu Gly Leu Glu Ser Lys Gly Ala Lys Val Tyr
65 70 75 80
ata aac tca cca gtt gag tct att gac ttt gac ggt aaa act gtt aca 288
Ile Asn Ser Pro Val Glu Ser Ile Asp Phe Asp Gly Lys Thr Val Thr
85 90 95
gct ctt gtt gat ggt aaa gaa cat gtt gaa tca tat gac aaa ttg att 336
Ala Leu Val Asp Gly Lys Glu His Val Glu Ser Tyr Asp Lys Leu Ile
100 105 110
tta gct act ggt tca caa cca atc tta cct cca atc gat ggt gct gag 384
Leu Ala Thr Gly Ser Gln Pro Ile Leu Pro Pro Ile Asp Gly Ala Glu
115 120 125
att aaa gaa ggt agc cgt act ttc gaa gcg act ctt gaa aac ctt caa 432
Ile Lys Glu Gly Ser Arg Thr Phe Glu Ala Thr Leu Glu Asn Leu Gln
130 135 140
ttc gtt aaa ctt ttc caa aac gct caa gaa gtt att gat aag ttg aac 480
Phe Val Lys Leu Phe Gln Asn Ala Gln Glu Val Ile Asp Lys Leu Asn
145 150 155 160
gat aaa tca caa gac atc aaa cgt gta gca gtc gtt ggt gca ggt tac 528
Asp Lys Ser Gln Asp Ile Lys Arg Val Ala Val Val Gly Ala Gly Tyr
165 170 175
att ggt gtt gaa ttg gct gaa gcc ttc caa cgt cat ggt aaa gaa gtt 576
Ile Gly Val Glu Leu Ala Glu Ala Phe Gln Arg His Gly Lys Glu Val
180 185 190
atc ctt att gat gtt gtt gac aca tgt ttg gca gga tac tat gac cgc 624
Ile Leu Ile Asp Val Val Asp Thr Cys Leu Ala Gly Tyr Tyr Asp Arg
195 200 205
gat ttt aca gac cgt atg gct aaa aat ttg gaa gac cac ggt atc caa 672
Asp Phe Thr Asp Arg Met Ala Lys Asn Leu Glu Asp His Gly Ile Gln
210 215 220
ttg gcc ttt ggc gaa act gtt aaa gca gta gaa ggt gaa act aag gta 720
Leu Ala Phe Gly Glu Thr Val Lys Ala Val Glu Gly Glu Thr Lys Val
225 230 235 240
gaa cgc atc gta aca gat aaa aat gcg tac gat gta gat atg gtt gtt 768
Glu Arg Ile Val Thr Asp Lys Asn Ala Tyr Asp Val Asp Met Val Val
245 250 255
ctt gct gtt ggt ttc cgt cct aat aca gcc ctt gga gct ggt aaa ttg 816
Leu Ala Val Gly Phe Arg Pro Asn Thr Ala Leu Gly Ala Gly Lys Leu
260 265 270
gaa aca ttc cga aat ggt gct tac ctt gtt aat aag aaa caa gaa aca 864
Glu Thr Phe Arg Asn Gly Ala Tyr Leu Val Asn Lys Lys Gln Glu Thr
275 280 285
tca gtt aag gat gtt tac gct gtt ggt gat tgt gct acg gtt tat gac 912
Ser Val Lys Asp Val Tyr Ala Val Gly Asp Cys Ala Thr Val Tyr Asp
290 295 300
aat gct ctt gac gat gtc aac tac att gcc tta gct tca aat gct att 960
Asn Ala Leu Asp Asp Val Asn Tyr Ile Ala Leu Ala Ser Asn Ala Ile
305 310 315 320
cgt tca ggt att gtc gct gga cac aat gcc ggt ggt ggt gat gtt gaa 1008
Arg Ser Gly Ile Val Ala Gly His Asn Ala Gly Gly Gly Asp Val Glu
325 330 335
tca aac ggt gtt caa gga tca aat ggt atc tct atc tat ggt ttg aac 1056
Ser Asn Gly Val Gln Gly Ser Asn Gly Ile Ser Ile Tyr Gly Leu Asn
340 345 350
atg gtt tct act ggt ttg act gaa gag aag gca aaa cgc ttt ggt ttc 1104
Met Val Ser Thr Gly Leu Thr Glu Glu Lys Ala Lys Arg Phe Gly Phe
355 360 365
aat cca gca gta gta gaa tca acg gat ctt caa aaa cct ccc ttc atg 1152
Asn Pro Ala Val Val Glu Ser Thr Asp Leu Gln Lys Pro Pro Phe Met
370 375 380
aag gat gaa aat gaa gat gta act atc aag att gtt tat gat aaa gat 1200
Lys Asp Glu Asn Glu Asp Val Thr Ile Lys Ile Val Tyr Asp Lys Asp
385 390 395 400
act cgt aaa gtt ttg ggt gct caa atg gtt tca ctc atg gat atc tca 1248
Thr Arg Lys Val Leu Gly Ala Gln Met Val Ser Leu Met Asp Ile Ser
405 410 415
atg gga atc cac atg ttc tca ttg gca att caa gaa gga gta aca att 1296
Met Gly Ile His Met Phe Ser Leu Ala Ile Gln Glu Gly Val Thr Ile
420 425 430
gac cgt cta caa ttg ctt gat ttg ttc ttc ttg cca cac ttt aac caa 1344
Asp Arg Leu Gln Leu Leu Asp Leu Phe Phe Leu Pro His Phe Asn Gln
435 440 445
cca ctt agc tat att gct aaa gca gct atc tcc gct gaa taa 1386
Pro Leu Ser Tyr Ile Ala Lys Ala Ala Ile Ser Ala Glu
450 455 460
<210> 130
<211> 461
<212> PRT
<213> 嗜热链球菌（Streptococcus thermophilus）
<400> 130
Leu Asp Lys Phe Met Ser Lys Ile Val Val Val Gly Ala Asn His Ala
1 5 10 15
Gly Thr Tyr Ser Ile Asn Thr Ile Leu Asp Asn Tyr Gly Asp Gln Asn
20 25 30
Glu Val Val Val Phe Asp Gln Asn Ser Asn Ile Ser Phe Leu Gly Cys
35 40 45
Gly Met Ala Leu Trp Ile Gly Asn Gln Ile Ser Gly Pro Asp Gly Leu
50 55 60
Phe Tyr Ala Asn Lys Glu Gly Leu Glu Ser Lys Gly Ala Lys Val Tyr
65 70 75 80
Ile Asn Ser Pro Val Glu Ser Ile Asp Phe Asp Gly Lys Thr Val Thr
85 90 95
Ala Leu Val Asp Gly Lys Glu His Val Glu Ser Tyr Asp Lys Leu Ile
100 105 110
Leu Ala Thr Gly Ser Gln Pro Ile Leu Pro Pro Ile Asp Gly Ala Glu
115 120 125
Ile Lys Glu Gly Ser Arg Thr Phe Glu Ala Thr Leu Glu Asn Leu Gln
130 135 140
Phe Val Lys Leu Phe Gln Asn Ala Gln Glu Val Ile Asp Lys Leu Asn
145 150 155 160
Asp Lys Ser Gln Asp Ile Lys Arg Val Ala Val Val Gly Ala Gly Tyr
165 170 175
Ile Gly Val Glu Leu Ala Glu Ala Phe Gln Arg His Gly Lys Glu Val
180 185 190
Ile Leu Ile Asp Val Val Asp Thr Cys Leu Ala Gly Tyr Tyr Asp Arg
195 200 205
Asp Phe Thr Asp Arg Met Ala Lys Asn Leu Glu Asp His Gly Ile Gln
210 215 220
Leu Ala Phe Gly Glu Thr Val Lys Ala Val Glu Gly Glu Thr Lys Val
225 230 235 240
Glu Arg Ile Val Thr Asp Lys Asn Ala Tyr Asp Val Asp Met Val Val
245 250 255
Leu Ala Val Gly Phe Arg Pro Asn Thr Ala Leu Gly Ala Gly Lys Leu
260 265 270
Glu Thr Phe Arg Asn Gly Ala Tyr Leu Val Asn Lys Lys Gln Glu Thr
275 280 285
Ser Val Lys Asp Val Tyr Ala Val Gly Asp Cys Ala Thr Val Tyr Asp
290 295 300
Asn Ala Leu Asp Asp Val Asn Tyr Ile Ala Leu Ala Ser Asn Ala Ile
305 310 315 320
Arg Ser Gly Ile Val Ala Gly His Asn Ala Gly Gly Gly Asp Val Glu
325 330 335
Ser Asn Gly Val Gln Gly Ser Asn Gly Ile Ser Ile Tyr Gly Leu Asn
340 345 350
Met Val Ser Thr Gly Leu Thr Glu Glu Lys Ala Lys Arg Phe Gly Phe
355 360 365
Asn Pro Ala Val Val Glu Ser Thr Asp Leu Gln Lys Pro Pro Phe Met
370 375 380
Lys Asp Glu Asn Glu Asp Val Thr Ile Lys Ile Val Tyr Asp Lys Asp
385 390 395 400
Thr Arg Lys Val Leu Gly Ala Gln Met Val Ser Leu Met Asp Ile Ser
405 410 415
Met Gly Ile His Met Phe Ser Leu Ala Ile Gln Glu Gly Val Thr Ile
420 425 430
Asp Arg Leu Gln Leu Leu Asp Leu Phe Phe Leu Pro His Phe Asn Gln
435 440 445
Pro Leu Ser Tyr Ile Ala Lys Ala Ala Ile Ser Ala Glu
450 455 460
<210> 131
<211> 1992
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(1992)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_0725 编码形成H2O的NADH氧化酶
<400> 131
atg aaa tac aaa aag cta ttt gaa act gtg aaa ata agg aat gtg gaa 48
Met Lys Tyr Lys Lys Leu Phe Glu Thr Val Lys Ile Arg Asn Val Glu
1 5 10 15
ctc aaa aat cgt tat gca atg gca cca atg ggt ccg ctg ggt ctt gcc 96
Leu Lys Asn Arg Tyr Ala Met Ala Pro Met Gly Pro Leu Gly Leu Ala
20 25 30
gat gca gaa ggc ggt ttc aac cag cgc ggg att gag tat tat aca gcc 144
Asp Ala Glu Gly Gly Phe Asn Gln Arg Gly Ile Glu Tyr Tyr Thr Ala
35 40 45
cgt gcg cgc ggg gga acc gct ctg att att acc ggc gtc act ttc gtt 192
Arg Ala Arg Gly Gly Thr Ala Leu Ile Ile Thr Gly Val Thr Phe Val
50 55 60
gat aat gaa gtg gaa gag cac gga atg cca aac gta cct tgc ccg acc 240
Asp Asn Glu Val Glu Glu His Gly Met Pro Asn Val Pro Cys Pro Thr
65 70 75 80
cat aac cct gtc cat ttt gtc cgg act tcc aaa gaa atg aca gag cgc 288
His Asn Pro Val His Phe Val Arg Thr Ser Lys Glu Met Thr Glu Arg
85 90 95
atc cat gca tat gat tcg aaa att ttt ctg caa atg agc gcc ggt ttt 336
Ile His Ala Tyr Asp Ser Lys Ile Phe Leu Gln Met Ser Ala Gly Phe
100 105 110
ggc cgg gtg acg atc ccg aca aac ctt ggc gag tac ccg ccg gtt gca 384
Gly Arg Val Thr Ile Pro Thr Asn Leu Gly Glu Tyr Pro Pro Val Ala
115 120 125
ccg tcg cca atc ccg cat cgc tgg ctg gat aaa aca tgt cgc gaa ctg 432
Pro Ser Pro Ile Pro His Arg Trp Leu Asp Lys Thr Cys Arg Glu Leu
130 135 140
aca gtt gaa gaa att cat tcc att gtc cgc aaa ttc ggg gat ggg gcg 480
Thr Val Glu Glu Ile His Ser Ile Val Arg Lys Phe Gly Asp Gly Ala
145 150 155 160
ttc aat gcg aag cgc gcc gga ttt gac ggg gtg caa atc cat gct gtg 528
Phe Asn Ala Lys Arg Ala Gly Phe Asp Gly Val Gln Ile His Ala Val
165 170 175
cac gaa ggc tat ttg ctc gac cag ttt gcg att gcg ttt ttc aac aaa 576
His Glu Gly Tyr Leu Leu Asp Gln Phe Ala Ile Ala Phe Phe Asn Lys
180 185 190
cgt acc gat gca tac ggt ggc ccg ctt gaa aat cgc ctt cgt ttt gcc 624
Arg Thr Asp Ala Tyr Gly Gly Pro Leu Glu Asn Arg Leu Arg Phe Ala
195 200 205
cgg gaa att gtc gag gaa att aaa cag cgc tgt ggc gaa gat ttt cct 672
Arg Glu Ile Val Glu Glu Ile Lys Gln Arg Cys Gly Glu Asp Phe Pro
210 215 220
gtg acg ctc cgc ttc agc ccg aaa agt ttt atc aag gat tgg cgg gaa 720
Val Thr Leu Arg Phe Ser Pro Lys Ser Phe Ile Lys Asp Trp Arg Glu
225 230 235 240
ggg gca ctg cct ggc gag gag ttt gaa gaa aaa ggc cgc gat ttg gat 768
Gly Ala Leu Pro Gly Glu Glu Phe Glu Glu Lys Gly Arg Asp Leu Asp
245 250 255
gaa ggc atc gag gca gca aag ctg ctc gtt tcc tac ggc tat gat gct 816
Glu Gly Ile Glu Ala Ala Lys Leu Leu Val Ser Tyr Gly Tyr Asp Ala
260 265 270
ctg gac gtc gat gtt ggt tct tat gat tca tgg tgg tgg agc cat cct 864
Leu Asp Val Asp Val Gly Ser Tyr Asp Ser Trp Trp Trp Ser His Pro
275 280 285
ccg atg tac cag aag aag ggg ctt tac att ccg tat gcc agg ctg gtg 912
Pro Met Tyr Gln Lys Lys Gly Leu Tyr Ile Pro Tyr Ala Arg Leu Val
290 295 300
aag gaa gct gtc gat gtg cct gtc ctt tgc gcg ggc cgc atg gac aat 960
Lys Glu Ala Val Asp Val Pro Val Leu Cys Ala Gly Arg Met Asp Asn
325 330 335
ccg gat ctt gca ctt gcc gca ctg gaa gac gga gca tgt gat att atc 1008
Pro Asp Leu Ala Leu Ala Ala Leu Glu Asp Gly Ala Cys Asp Ile Ile
325 330 335
agc ttg ggc cgc ccg tta ttg gct gac ccg gat tac gtc aat aag ctc 1056
Ser Leu Gly Arg Pro Leu Leu Ala Asp Pro Asp Tyr Val Asn Lys Leu
340 345 350
cga atc ggg cag gtt gcc gat atc cgc ccg tgt ctg tca tgc cat gaa 1104
Arg Ile Gly Gln Val Ala Asp Ile Arg Pro Cys Leu Ser Cys His Glu
355 360 365
ggc tgc atg ggt cgg atc cag gag tat tct tcc tta ggc tgc gca gtg 1152
Gly Cys Met Gly Arg Ile Gln Glu Tyr Ser Ser Leu Gly Cys Ala Val
370 375 380
aat ccg gct gcc tgt cga gaa aaa gaa gca gca ttg aca cct gct tta 1200
Asn Pro Ala Ala Cys Arg Glu Lys Glu Ala Ala Leu Thr Pro Ala Leu
385 390 395 400
aaa aag aaa cgc gta ctg att gca ggc ggc ggc gtg gcc gga tgc gaa 1248
Lys Lys Lys Arg Val Leu Ile Ala Gly Gly Gly Val Ala Gly Cys Glu
405 410 415
gct gcc cgt gtg ctt gca ttg cgc ggc cat gaa ccg gtc att ttt gaa 1296
Ala Ala Arg Val Leu Ala Leu Arg Gly His Glu Pro Val Ile Phe Glu
420 425 430
aaa tcg aac cgt tta ggc ggc aac ttg atc cct ggc ggc gca cct gat 1344
Lys Ser Asn Arg Leu Gly Gly Asn Leu Ile Pro Gly Gly Ala Pro Asp
435 440 445
ttt aaa gaa gat gac ctg gcg ctt gtt gcc tgg tat gag cat acg ttg 1392
Phe Lys Glu Asp Asp Leu Ala Leu Val Ala Trp Tyr Glu His Thr Leu
450 455 460
gaa cgc ctt ggc gta gaa att cat ttg aat act gca ttg aca aaa gaa 1440
Glu Arg Leu Gly Val Glu Ile His Leu Asn Thr Ala Leu Thr Lys Glu
465 470 475 480
gaa att ttg gct gca aac gtg gat gcc gtg ctg att gca acg ggt tcg 1488
Glu Ile Leu Ala Ala Asn Val Asp Ala Val Leu Ile Ala Thr Gly Ser
485 490 495
aat ccg aaa att ttg ccg ctc gac gga aaa aac aaa gta ttt aca gca 1536
Asn Pro Lys Ile Leu Pro Leu Asp Gly Lys Asn Lys Val Phe Thr Ala
500 505 510
gaa gat gtt ttg ctc gat aaa gtg gat gcc ggg caa cat gtt gtc att 1584
Glu Asp Val Leu Leu Asp Lys Val Asp Ala Gly Gln His Val Val Ile
515 520 525
gtc ggc ggc ggt ctt gtc ggc tgc gaa ctg gct ttg aac ctt gca gaa 1632
Val Gly Gly Gly Leu Val Gly Cys Glu Leu Ala Leu Asn Leu Ala Glu
530 535 540
aaa gga aaa gat gtc tcg ctt gtg gaa atg cag gac aaa ctg ctg gca 1680
Lys Gly Lys Asp Val Ser Leu Val Glu Met Gln Asp Lys Leu Leu Ala
545 550 555 560
gtt aat ggt ccg ctt tgc cac gct aac tcg gac atg ctg gaa aga ctc 1728
Val Asn Gly Pro Leu Cys His Ala Asn Ser Asp Met Leu Glu Arg Leu
565 570 575
gta ccg ttt aaa ggt gtt caa gtc tac act tct tca aaa ata gta gat 1776
Val Pro Phe Lys Gly Val Gln Val Tyr Thr Ser Ser Lys Ile Val Asp
580 585 590
acg aca gaa aag aca gcc gtt gtg gat gtt gac ggc gaa ttg cgt gaa 1824
Thr Thr Glu Lys Thr Ala Val Val Asp Val Asp Gly Glu Leu Arg Glu
595 600 605
att gaa gca gac agc att gtg ctc gca gtc ggc tac tcg gct gaa aaa 1872
Ile Glu Ala Asp Ser Ile Val Leu Ala Val Gly Tyr Ser Ala Glu Lys
610 615 620
tca ctc tat gaa gat tta aag ttt gag gtt gcc gat ctt cat gtg gtt 1920
Ser Leu Tyr Glu Asp Leu Lys Phe Glu Val Ala Asp Leu His Val Val
625 630 635 640
ggc gat gcc cgc aag gtc gca aac atc atg tat gcc atc tgg gat gct 1968
Gly Asp Ala Arg Lys Val Ala Asn Ile Met Tyr Ala Ile Trp Asp Ala
645 650 655
tac gaa gtc gcg gca aat ctg taa 1992
Tyr Glu Val Ala Ala Asn Leu
660
<210> 132
<211> 663
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 132
Met Lys Tyr Lys Lys Leu Phe Glu Thr Val Lys Ile Arg Asn Val Glu
1 5 10 15
Leu Lys Asn Arg Tyr Ala Met Ala Pro Met Gly Pro Leu Gly Leu Ala
20 25 30
Asp Ala Glu Gly Gly Phe Asn Gln Arg Gly Ile Glu Tyr Tyr Thr Ala
35 40 45
Arg Ala Arg Gly Gly Thr Ala Leu Ile Ile Thr Gly Val Thr Phe Val
50 55 60
Asp Asn Glu Val Glu Glu His Gly Met Pro Asn Val Pro Cys Pro Thr
65 70 75 80
His Asn Pro Val His Phe Val Arg Thr Ser Lys Glu Met Thr Glu Arg
85 90 95
Ile His Ala Tyr Asp Ser Lys Ile Phe Leu Gln Met Ser Ala Gly Phe
100 105 110
Gly Arg Val Thr Ile Pro Thr Asn Leu Gly Glu Tyr Pro Pro Val Ala
115 120 125
Pro Ser Pro Ile Pro His Arg Trp Leu Asp Lys Thr Cys Arg Glu Leu
130 135 140
Thr Val Glu Glu Ile His Ser Ile Val Arg Lys Phe Gly Asp Gly Ala
145 150 155 160
Phe Asn Ala Lys Arg Ala Gly Phe Asp Gly Val Gln Ile His Ala Val
165 170 175
His Glu Gly Tyr Leu Leu Asp Gln Phe Ala Ile Ala Phe Phe Asn Lys
180 185 190
Arg Thr Asp Ala Tyr Gly Gly Pro Leu Glu Asn Arg Leu Arg Phe Ala
195 200 205
Arg Glu Ile Val Glu Glu Ile Lys Gln Arg Cys Gly Glu Asp Phe Pro
210 215 220
Val Thr Leu Arg Phe Ser Pro Lys Ser Phe Ile Lys Asp Trp Arg Glu
225 230 235 240
Gly Ala Leu Pro Gly Glu Glu Phe Glu Glu Lys Gly Arg Asp Leu Asp
245 250 255
Glu Gly Ile Glu Ala Ala Lys Leu Leu Val Ser Tyr Gly Tyr Asp Ala
260 265 270
Leu Asp Val Asp Val Gly Ser Tyr Asp Ser Trp Trp Trp Ser His Pro
275 280 285
Pro Met Tyr Gln Lys Lys Gly Leu Tyr Ile Pro Tyr Ala Arg Leu Val
290 295 300
Lys Glu Ala Val Asp Val Pro Val Leu Cys Ala Gly Arg Met Asp Asn
305 310 315 320
Pro Asp Leu Ala Leu Ala Ala Leu Glu Asp Gly Ala Cys Asp Ile Ile
325 330 335
Ser Leu Gly Arg Pro Leu Leu Ala Asp Pro Asp Tyr Val Asn Lys Leu
340 345 350
Arg Ile Gly Gln Val Ala Asp Ile Arg Pro Cys Leu Ser Cys His Glu
355 360 365
Gly Cys Met Gly Arg Ile Gln Glu Tyr Ser Ser Leu Gly Cys Ala Val
370 375 380
Asn Pro Ala Ala Cys Arg Glu Lys Glu Ala Ala Leu Thr Pro Ala Leu
385 390 395 400
Lys Lys Lys Arg Val Leu Ile Ala Gly Gly Gly Val Ala Gly Cys Glu
405 410 415
Ala Ala Arg Val Leu Ala Leu Arg Gly His Glu Pro Val Ile Phe Glu
420 425 430
Lys Ser Asn Arg Leu Gly Gly Asn Leu Ile Pro Gly Gly Ala Pro Asp
435 440 445
Phe Lys Glu Asp Asp Leu Ala Leu Val Ala Trp Tyr Glu His Thr Leu
450 455 460
Glu Arg Leu Gly Val Glu Ile His Leu Asn Thr Ala Leu Thr Lys Glu
465 470 475 480
Glu Ile Leu Ala Ala Asn Val Asp Ala Val Leu Ile Ala Thr Gly Ser
485 490 495
Asn Pro Lys Ile Leu Pro Leu Asp Gly Lys Asn Lys Val Phe Thr Ala
500 505 510
Glu Asp Val Leu Leu Asp Lys Val Asp Ala Gly Gln His Val Val Ile
515 520 525
Val Gly Gly Gly Leu Val Gly Cys Glu Leu Ala Leu Asn Leu Ala Glu
530 535 540
Lys Gly Lys Asp Val Ser Leu Val Glu Met Gln Asp Lys Leu Leu Ala
545 550 555 560
Val Asn Gly Pro Leu Cys His Ala Asn Ser Asp Met Leu Glu Arg Leu
565 570 575
Val Pro Phe Lys Gly Val Gln Val Tyr Thr Ser Ser Lys Ile Val Asp
580 585 590
Thr Thr Glu Lys Thr Ala Val Val Asp Val Asp Gly Glu Leu Arg Glu
595 600 605
Ile Glu Ala Asp Ser Ile Val Leu Ala Val Gly Tyr Ser Ala Glu Lys
610 615 620
Ser Leu Tyr Glu Asp Leu Lys Phe Glu Val Ala Asp Leu His Val Val
625 630 635 640
Gly Asp Ala Arg Lys Val Ala Asn Ile Met Tyr Ala Ile Trp Asp Ala
645 650 655
Tyr Glu Val Ala Ala Asn Leu
660
<210> 133
<211> 1119
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(1119)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_0915 编码形成H2O的NADH氧化酶
<400> 133
atg agc aaa tac gat aaa ctg ttt gaa cca att acg ttt aaa agc ggc 48
Met Ser Lys Tyr Asp Lys Leu Phe Glu Pro Ile Thr Phe Lys Ser Gly
1 5 10 15
gtc agg gcg gaa aac cgc atc gtg atg gcg ccg atg acc cat tat tcc 96
Val Arg Ala Glu Asn Arg Ile Val Met Ala Pro Met Thr His Tyr Ser
20 25 30
tcg aat gat gac ggc acg att tcc gct gcc gag ctg cgt tat tat gca 144
Ser Asn Asp Asp Gly Thr Ile Ser Ala Ala Glu Leu Arg Tyr Tyr Ala
35 40 45
gaa cgt gcc aaa ggg ccg ggg atg gtc gtg acg gca tgt gcc aat gtt 192
Glu Arg Ala Lys Gly Pro Gly Met Val Val Thr Ala Cys Ala Asn Val
50 55 60
acg gaa aac ggc aaa gca ttt acc ggg caa cct ggc gcg cat aca gat 240
Thr Glu Asn Gly Lys Ala Phe Thr Gly Gln Pro Gly Ala His Thr Asp
65 70 75 80
gaa atg gtt gag agc atg aaa aaa cta gca caa acg att cag caa aac 288
Glu Met Val Glu Ser Met Lys Lys Leu Ala Gln Thr Ile Gln Gln Asn
85 90 95
ggt gca aaa gcg gtt gtg caa atc cac cat ggt ggc cgc gaa tgc ccg 336
Gly Ala Lys Ala Val Val Gln Ile His His Gly Gly Arg Glu Cys Pro
100 105 110
gct gaa ctc gtg ccg ggc ggg gat gtt gtg agc gca agt gaa aca gat 384
Ala Glu Leu Val Pro Gly Gly Asp Val Val Ser Ala Ser Glu Thr Asp
115 120 125
tcg tca gac ggc agc gtc cat gca cgc gcg ttg acg gga gaa gaa acc 432
Ser Ser Asp Gly Ser Val His Ala Arg Ala Leu Thr Gly Glu Glu Thr
130 135 140
gct gaa att gtg aaa gca ttc ggc gaa gca aca cgc cgc gtg atc gaa 480
Ala Glu Ile Val Lys Ala Phe Gly Glu Ala Thr Arg Arg Val Ile Glu
145 150 155 160
gct ggt ttt gat ggc gtt gaa att cac ggc gca aac ggc tat ctg att 528
Ala Gly Phe Asp Gly Val Glu Ile His Gly Ala Asn Gly Tyr Leu Ile
165 170 175
caa caa ttt ttc tcc ccg tat acg aac cgg cgt gaa gac gag tgg ggc 576
Gln Gln Phe Phe Ser Pro Tyr Thr Asn Arg Arg Glu Asp Glu Trp Gly
180 185 190
ggc agt ctg gaa aaa cgg ctt gca ttc ccg ctt gct gtc gtc gat gct 624
Gly Ser Leu Glu Lys Arg Leu Ala Phe Pro Leu Ala Val Val Asp Ala
195 200 205
gtg aaa aaa acg gtg gcg gaa tat gcg gac cgg ccg ttt tta gtc gga 672
Val Lys Lys Thr Val Ala Glu Tyr Ala Asp Arg Pro Phe Leu Val Gly
210 215 220
tac cgc tta tcc ccg gaa gaa ccg tac gaa ccg ggg att acg atg gat 720
Tyr Arg Leu Ser Pro Glu Glu Pro Tyr Glu Pro Gly Ile Thr Met Asp
225 230 235 240
gac acg ttc gcg ctt ttg gat gcg ctg atg aaa aaa gag ctc gac tat 768
Asp Thr Phe Ala Leu Leu Asp Ala Leu Met Lys Lys Glu Leu Asp Tyr
245 250 255
gtg cat att tcc ctg cag cac ttc tgg tct aaa gcc cgc cgc ggc ggt 816
Val His Ile Ser Leu Gln His Phe Trp Ser Lys Ala Arg Arg Gly Gly
260 265 270
gaa ccc ggc cgt ccg cgc atg gaa cag atc gtg gaa cat gtc gca ggc 864
Glu Pro Gly Arg Pro Arg Met Glu Gln Ile Val Glu His Val Ala Gly
275 280 285
cgc gtg cct ttg atc ggc gtc ggc tcc ctg cac aca gga gac gag gtt 912
Arg Val Pro Leu Ile Gly Val Gly Ser Leu His Thr Gly Asp Glu Val
290 295 300
ttg aag gca atg gaa acc gat gta ccg ctt att gcc ctc ggc cgt gag 960
Leu Lys Ala Met Glu Thr Asp Val Pro Leu Ile Ala Leu Gly Arg Glu
305 310 315 320
ctt gtc atg gag ccg cat tgg gtg gaa aaa att aaa gcc ggc cag gag 1008
Leu Val Met Glu Pro His Trp Val Glu Lys Ile Lys Ala Gly Gln Glu
325 330 335
gat gca att caa acc gcg tta aga aaa gac gac cgc gaa cgg ctc gtt 1056
Asp Ala Ile Gln Thr Ala Leu Arg Lys Asp Asp Arg Glu Arg Leu Val
340 345 350
att ccg gaa ccg ctg tgg aag atg atc atc tca acg ccg ggc tgg ttc 1104
Ile Pro Glu Pro Leu Trp Lys Met Ile Ile Ser Thr Pro Gly Trp Phe
355 360 365
ccg ttt gca gaa tga 1119
Pro Phe Ala Glu
370
<210> 134
<211> 372
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 134
Met Ser Lys Tyr Asp Lys Leu Phe Glu Pro Ile Thr Phe Lys Ser Gly
1 5 10 15
Val Arg Ala Glu Asn Arg Ile Val Met Ala Pro Met Thr His Tyr Ser
20 25 30
Ser Asn Asp Asp Gly Thr Ile Ser Ala Ala Glu Leu Arg Tyr Tyr Ala
35 40 45
Glu Arg Ala Lys Gly Pro Gly Met Val Val Thr Ala Cys Ala Asn Val
50 55 60
Thr Glu Asn Gly Lys Ala Phe Thr Gly Gln Pro Gly Ala His Thr Asp
65 70 75 80
Glu Met Val Glu Ser Met Lys Lys Leu Ala Gln Thr Ile Gln Gln Asn
85 90 95
Gly Ala Lys Ala Val Val Gln Ile His His Gly Gly Arg Glu Cys Pro
100 105 110
Ala Glu Leu Val Pro Gly Gly Asp Val Val Ser Ala Ser Glu Thr Asp
115 120 125
Ser Ser Asp Gly Ser Val His Ala Arg Ala Leu Thr Gly Glu Glu Thr
130 135 140
Ala Glu Ile Val Lys Ala Phe Gly Glu Ala Thr Arg Arg Val Ile Glu
145 150 155 160
Ala Gly Phe Asp Gly Val Glu Ile His Gly Ala Asn Gly Tyr Leu Ile
165 170 175
Gln Gln Phe Phe Ser Pro Tyr Thr Asn Arg Arg Glu Asp Glu Trp Gly
180 185 190
Gly Ser Leu Glu Lys Arg Leu Ala Phe Pro Leu Ala Val Val Asp Ala
195 200 205
Val Lys Lys Thr Val Ala Glu Tyr Ala Asp Arg Pro Phe Leu Val Gly
210 215 220
Tyr Arg Leu Ser Pro Glu Glu Pro Tyr Glu Pro Gly Ile Thr Met Asp
225 230 235 240
Asp Thr Phe Ala Leu Leu Asp Ala Leu Met Lys Lys Glu Leu Asp Tyr
245 250 255
Val His Ile Ser Leu Gln His Phe Trp Ser Lys Ala Arg Arg Gly Gly
260 265 270
Glu Pro Gly Arg Pro Arg Met Glu Gln Ile Val Glu His Val Ala Gly
275 280 285
Arg Val Pro Leu Ile Gly Val Gly Ser Leu His Thr Gly Asp Glu Val
290 295 300
Leu Lys Ala Met Glu Thr Asp Val Pro Leu Ile Ala Leu Gly Arg Glu
305 310 315 320
Leu Val Met Glu Pro His Trp Val Glu Lys Ile Lys Ala Gly Gln Glu
325 330 335
Asp Ala Ile Gln Thr Ala Leu Arg Lys Asp Asp Arg Glu Arg Leu Val
340 345 350
Ile Pro Glu Pro Leu Trp Lys Met Ile Ile Ser Thr Pro Gly Trp Phe
355 360 365
Pro Phe Ala Glu
370
<210> 135
<211> 1032
<212> DNA
<213> 凝结芽孢杆菌（Bacillus coagulans）
<220>
<221> CDS
<222> (1)..(1032)
<223> 凝结芽孢杆菌 36D1; 基因 Bcoa_2324 编码形成H2O的NADH氧化酶
<400> 135
atg aat gat tta ttt acc ccg tac aag ctg aaa tcg ctt gaa gtg aaa 48
Met Asn Asp Leu Phe Thr Pro Tyr Lys Leu Lys Ser Leu Glu Val Lys
1 5 10 15
aac cgt att gtc atg cct ccc atg tgc caa tat tcc gtt gag aaa aaa 96
Asn Arg Ile Val Met Pro Pro Met Cys Gln Tyr Ser Val Glu Lys Lys
20 25 30
gac ggc gtt gcc act gac tgg cat tat gtc cat tac gtc agc cgt gcc 144
Asp Gly Val Ala Thr Asp Trp His Tyr Val His Tyr Val Ser Arg Ala
35 40 45
atc ggg ggc acc ggc ctg atc att atg gag atg acc gat gtg gag ccg 192
Ile Gly Gly Thr Gly Leu Ile Ile Met Glu Met Thr Asp Val Glu Pro
50 55 60
gac ggc aga att acc gac aac gat ctc ggc tta tgg tct gat gag cag 240
Asp Gly Arg Ile Thr Asp Asn Asp Leu Gly Leu Trp Ser Asp Glu Gln
65 70 75 80
atc gcg ccg ctt gcc aaa att gtg gaa gcg tgc cac cag tac ggc gcc 288
Ile Ala Pro Leu Ala Lys Ile Val Glu Ala Cys His Gln Tyr Gly Ala
85 90 95
aaa gtc ggg att caa atc gga cat gcc ggc aga aaa gca cag gat gca 336
Lys Val Gly Ile Gln Ile Gly His Ala Gly Arg Lys Ala Gln Asp Ala
100 105 110
aaa caa cca gtt gcc cct tcc ccc atc cct ttt gat gac cat gca aaa 384
Lys Gln Pro Val Ala Pro Ser Pro Ile Pro Phe Asp Asp His Ala Lys
115 120 125
aca ccg agg gca tta acg aag gaa gaa atc aaa gaa atc gtc cgc aaa 432
Thr Pro Arg Ala Leu Thr Lys Glu Glu Ile Lys Glu Ile Val Arg Lys
130 135 140
ttt ggc gaa tct gtc cgc cgc gct gtc cag gcc ggt ttt gac acg atc 480
Phe Gly Glu Ser Val Arg Arg Ala Val Gln Ala Gly Phe Asp Thr Ile
145 150 155 160
gaa ctg cac ggc gca cac ggt tat ctg atc cat gaa ttc cag tcg cgc 528
Glu Leu His Gly Ala His Gly Tyr Leu Ile His Glu Phe Gln Ser Arg
165 170 175
tat aca aac aaa cgg tca gat gaa tac gga cag gat ttg gca tta ttt 576
Tyr Thr Asn Lys Arg Ser Asp Glu Tyr Gly Gln Asp Leu Ala Leu Phe
180 185 190
gga aaa gaa gtc att gcc gct gcc aaa agc gaa atg cct gcc gac atg 624
Gly Lys Glu Val Ile Ala Ala Ala Lys Ser Glu Met Pro Ala Asp Met
195 200 205
cca ctt att atg cgc att tca gcc cgt gaa tat gtc gat ggc ggg tat 672
Pro Leu Ile Met Arg Ile Ser Ala Arg Glu Tyr Val Asp Gly Gly Tyr
210 215 220
ggc att gaa gaa agt att gcg ctt tcg aag gcg tat aaa gaa gcc ggt 720
Gly Ile Glu Glu Ser Ile Ala Leu Ser Lys Ala Tyr Lys Glu Ala Gly
225 230 235 240
gtt gac ctg ttt cat att agt tcc gga ggc gag ggc cat atc ggt gcg 768
Val Asp Leu Phe His Ile Ser Ser Gly Gly Glu Gly His Ile Gly Ala
245 250 255
gcc ggc cgc ccg ggg acg cac gcc gct tat cag gtg ccg ctt gca cgc 816
Ala Gly Arg Pro Gly Thr His Ala Ala Tyr Gln Val Pro Leu Ala Arg
260 265 270
cag atc aaa gag gct tta aac gtt ccg gtc att gca gtc ggc cgg ctg 864
Gln Ile Lys Glu Ala Leu Asn Val Pro Val Ile Ala Val Gly Arg Leu
275 280 285
gaa gac ccg gtt ctt gcc aat gcg gta atc gga aac gaa gaa gca gat 912
Glu Asp Pro Val Leu Ala Asn Ala Val Ile Gly Asn Glu Glu Ala Asp
290 295 300
ctt gtt gcg gtc ggc aga ggc atg ctc cgc aac cct tat tgg gcg ctc 960
Leu Val Ala Val Gly Arg Gly Met Leu Arg Asn Pro Tyr Trp Ala Leu
305 310 315 320
aat gca ggg atc gcg ctc ggc aaa gac gca ggt gtg ccg atc caa tat 1008
Asn Ala Gly Ile Ala Leu Gly Lys Asp Ala Gly Val Pro Ile Gln Tyr
325 330 335
aca agg ggg ttt cca aaa agg taa 1032
Thr Arg Gly Phe Pro Lys Arg
340
<210> 136
<211> 343
<212> PRT
<213> 凝结芽孢杆菌（Bacillus coagulans）
<400> 136
Met Asn Asp Leu Phe Thr Pro Tyr Lys Leu Lys Ser Leu Glu Val Lys
1 5 10 15
Asn Arg Ile Val Met Pro Pro Met Cys Gln Tyr Ser Val Glu Lys Lys
20 25 30
Asp Gly Val Ala Thr Asp Trp His Tyr Val His Tyr Val Ser Arg Ala
35 40 45
Ile Gly Gly Thr Gly Leu Ile Ile Met Glu Met Thr Asp Val Glu Pro
50 55 60
Asp Gly Arg Ile Thr Asp Asn Asp Leu Gly Leu Trp Ser Asp Glu Gln
65 70 75 80
Ile Ala Pro Leu Ala Lys Ile Val Glu Ala Cys His Gln Tyr Gly Ala
85 90 95
Lys Val Gly Ile Gln Ile Gly His Ala Gly Arg Lys Ala Gln Asp Ala
100 105 110
Lys Gln Pro Val Ala Pro Ser Pro Ile Pro Phe Asp Asp His Ala Lys
115 120 125
Thr Pro Arg Ala Leu Thr Lys Glu Glu Ile Lys Glu Ile Val Arg Lys
130 135 140
Phe Gly Glu Ser Val Arg Arg Ala Val Gln Ala Gly Phe Asp Thr Ile
145 150 155 160
Glu Leu His Gly Ala His Gly Tyr Leu Ile His Glu Phe Gln Ser Arg
165 170 175
Tyr Thr Asn Lys Arg Ser Asp Glu Tyr Gly Gln Asp Leu Ala Leu Phe
180 185 190
Gly Lys Glu Val Ile Ala Ala Ala Lys Ser Glu Met Pro Ala Asp Met
195 200 205
Pro Leu Ile Met Arg Ile Ser Ala Arg Glu Tyr Val Asp Gly Gly Tyr
210 215 220
Gly Ile Glu Glu Ser Ile Ala Leu Ser Lys Ala Tyr Lys Glu Ala Gly
225 230 235 240
Val Asp Leu Phe His Ile Ser Ser Gly Gly Glu Gly His Ile Gly Ala
245 250 255
Ala Gly Arg Pro Gly Thr His Ala Ala Tyr Gln Val Pro Leu Ala Arg
260 265 270
Gln Ile Lys Glu Ala Leu Asn Val Pro Val Ile Ala Val Gly Arg Leu
275 280 285
Glu Asp Pro Val Leu Ala Asn Ala Val Ile Gly Asn Glu Glu Ala Asp
290 295 300
Leu Val Ala Val Gly Arg Gly Met Leu Arg Asn Pro Tyr Trp Ala Leu
305 310 315 320
Asn Ala Gly Ile Ala Leu Gly Lys Asp Ala Gly Val Pro Ile Gln Tyr
325 330 335
Thr Arg Gly Phe Pro Lys Arg
340
<210> 137
<211> 20
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(20)
<223> 43 (T3) 引物
<400> 137
aattaaccct cactaaaggg 20
<210> 138
<211> 22
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(22)
<223> 引物 603
<400> 138
atcaaccttt gatacaaggt tg 22
<210> 139
<211> 108
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(108)
<223> 引物 710
<220>
<221> misc_feature（新的或少见的特征）
<222> (11)..(15)
<223> n is a, c, g, or t
<220>
<221> misc_feature（新的或少见的特征）
<222> (31)..(44)
<223> n is a, c, g, or t
<220>
<221> misc_feature（新的或少见的特征）
<222> (54)..(57)
<223> n is a, c, g, or t
<400> 139
ctagtctaga nnnnnagttt attcttgaca nnnnnnnnnn nnnntgrtat aatnnnnaag 60
taataaaata ttcggaggaa ttttgaaatg gctgataaac aacgtaag 108
<210> 140
<211> 37
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(37)
<223> 引物 768
<400> 140
aattcctgca gcatattaaa taatgaacaa gtcattc 37
<210> 141
<211> 32
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(32)
<223> 引物 769
<400> 141
tagtggatcc tggtaaatcc aaacacaaca ac 32
<210> 142
<211> 34
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(34)
<223> 引物 770
<400> 142
aattcctgca gtaatttcca gctcttacaa taac 34
<210> 143
<211> 31
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(31)
<223> 引物 771
<400> 143
gacctcgagt cagaaacttt ctttaccaga g 31
<210> 144
<211> 21
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(21)
<223> 引物 772
<400> 144
gcggggatcc actagttcta g 21
<210> 145
<211> 17
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(17)
<223> 引物 773
<400> 145
ataccgtcga cctcgag 17
<210> 146
<211> 30
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(30)
<223> 引物 774
<400> 146
tagtggatcc ctgtttcagg tcttggatag 30
<210> 147
<211> 33
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(33)
<223> 引物 775
<400> 147
ccgatgaatt ctcattagca cgtttaacaa gag 33
<210> 148
<211> 29
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(29)
<223> 引物 776
<400> 148
ccgatgaatt catcagcgta gtctgctgc 29
<210> 149
<211> 33
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(33)
<223> 引物 777
<400> 149
cggggtacca tttaatccta aagtcgttat tac 33
<210> 150
<211> 32
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(32)
<223> 引物 785
<400> 150
ccgatgaatt cttaagtcaa gacaacgagg tc 32
<210> 151
<211> 33
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(33)
<223> 引物 786
<400> 151
ccgatgaatt cgaccttgtt gaaaaaaatc ttc 33
<210> 152
<211> 30
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(30)
<223> 引物 787
<400> 152
tagtggatcc gtacaatggc tactgttaac 30
<210> 153
<211> 33
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(33)
<223> 引物 788
<400> 153
gacctcgagg atgaacagac ttttttatta tag 33
<210> 154
<211> 20
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(20)
<223> 引物 789
<400> 154
aaaaccaggt gaaactcgtc 20
<210> 155
<211> 38
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(38)
<223> 引物 791
<400> 155
tcggactgca gttaaaatgc tgataaaaac aattcttc 38
<210> 156
<211> 17
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(17)
<223> 引物 827
<400> 156
ataccgtcga cctcgag 17
<210> 157
<211> 22
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(22)
<223> 引物 828
<400> 157
cgataagctt gatatcgaat tc 22
<210> 158
<211> 52
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(52)
<223> 引物 830
<400> 158
ccgatgaatt ctataaggag aattagaatg gcaagtagta cattttatat tc 52
<210> 159
<211> 21
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(21)
<223> 引物 878
<400> 159
atccctcggt tacaagtttc u 21
<210> 160
<211> 45
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(45)
<223> 引物 879
<400> 160
agaaacttgt aaccgaggga uaataataga ttgaaattct gtcag 45
<210> 161
<211> 46
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(46)
<223> 引物 880
<400> 161
attcgatatc aagcttatcg aucaaaaatt gtggtagaat atatag 46
<210> 162
<211> 40
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(40)
<223> 引物 881
<400> 162
aggtcgacgg tatcgataau cctagttcaa ttgatgtgac 40
<210> 163
<211> 22
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(22)
<223> 引物 882
<400> 163
atcgataagc ttgatatcga au 22
<210> 164
<211> 20
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(20)
<223> 引物 883
<400> 164
attatcgata ccgtcgaccu 20
<210> 165
<211> 48
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(48)
<223> 引物 887
<400> 165
attcgatatc aagcttatcg auatttaaaa atgattgcaa catataac 48
<210> 166
<211> 24
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(24)
<223> 引物 888
<400> 166
ataggtctcc tttaaatgta aaau 24
<210> 167
<211> 46
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(46)
<223> 引物 889
<400> 167
attttacatt taaaggagac ctautagaaa tctatctgct tgatag 46
<210> 168
<211> 41
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(41)
<223> 引物 890
<400> 168
aggtcgacgg tatcgataac gucttcaccg tccattttga c 41
<210> 169
<211> 23
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(23)
<223> 引物 891
<400> 169
acagattaaa ggttgaccag tau 23
<210> 170
<211> 20
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(20)
<223> 引物 892
<400> 170
accaattctg tgttgcgcau 20
<210> 171
<211> 95
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(95)
<223> 引物 893
<220>
<221> misc_feature（新的或少见的特征）
<222> (25)..(29)
<223> n is a, c, g, t or u
<220>
<221> misc_feature（新的或少见的特征）
<222> (45)..(58)
<223> n is a, c, g, t or u
<220>
<221> misc_feature（新的或少见的特征）
<222> (68)..(71)
<223> n is a, c, g, t or u
<400> 171
atgcgcaaca cagaattggu ggccnnnnna gtttattctt gacannnnnn nnnnnnnntg 60
rtataatnnn naagtaataa aatattcgga ggaat 95
<210> 172
<211> 49
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(49)
<223> 引物 894
<400> 172
atactggtca acctttaatc tguttaaaat gctgataaaa acaattctt 49
<210> 173
<211> 22
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(22)
<223> 引物 920
<400> 173
ataagctuga tatcgaattc ct 22
<210> 174
<211> 22
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(22)
<223> 引物 921
<400> 174
attcccttua gtgagggtta at 22
<210> 175
<211> 38
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(38)
<223> 引物 926
<400> 175
tcgacctcga gttttttatt tttagttttt aactgcag 38
<210> 176
<211> 21
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(21)
<223> 引物 927
<400> 176
atgtgtacgu tctcctttgt g 21
<210> 177
<211> 32
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(32)
<223> 引物 928
<400> 177
acgtacacau attatagtat ttggaaccga ac 32
<210> 178
<211> 27
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(27)
<223> 引物 929
<400> 178
aagcttaugg tcgtcttgtt acttgtg 27
<210> 179
<211> 28
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(28)
<223> 引物 930
<400> 179
aaagggaaut ctgccggagc tatatatg 28
<210> 180
<211> 24
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(24)
<223> 引物 975
<400> 180
ttaattatac aacattcctc catc 24
<210> 181
<211> 32
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(32)
<223> 引物 976
<400> 181
aattcctgca gatctatacc tacttgacca gc 32
<210> 182
<211> 30
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(30)
<223> 引物 977
<400> 182
tagtggatcc gagtattcgc aaaccttcag 30
<210> 183
<211> 35
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(35)
<223> 引物 978
<400> 183
aattcctgca gaataaatga atgaggtaag gtcta 35
<210> 184
<211> 34
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(34)
<223> 引物 979
<400> 184
gacctcgagt ttaagagata aaaggttaat tgtg 34
<210> 185
<211> 57
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(57)
<223> 引物 991
<400> 185
gaatcggtac caataaaata ttcggaggaa ttttgaaatg ttacgtcctg tagaaac 57
<210> 186
<211> 68
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(68)
<223> 引物 992
<400> 186
ggaccgtacg ttaaaaaata aaaaagaacc cactcgggtt ctttttttta ttgtttgcct 60
ccctgctg 68
<210> 187
<211> 30
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(30)
<223> 引物 1057
<400> 187
tagtggatcc cttaattgct ggaatcactg 30
<210> 188
<211> 35
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(35)
<223> 引物 1058
<400> 188
aattcctgca gatgatattt ctcttttcta tctca 35
<210> 189
<211> 35
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(35)
<223> 引物 1059
<400> 189
aattcctgca gaattgctta aatttcttta gctac 35
<210> 190
<211> 30
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(30)
<223> 引物 1060
<400> 190
tcgacctcga gttagacgct cgggataaag 30
<210> 191
<211> 18
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(18)
<223> 引物 1112
<400> 191
gcaacaacgt gcgcaaac 18
<210> 192
<211> 17
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(17)
<223> 引物 1113
<400> 192
ctgcaggtcg actctag 17
<210> 193
<211> 22
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(22)
<223> 引物 1117
<400> 193
aatattttag gacccaatga tg 22
<210> 194
<211> 20
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(20)
<223> 引物 1119
<400> 194
cgagctggaa agcttttatc 20
<210> 195
<211> 109
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(109)
<223> 引物 1130
<220>
<221> misc_feature（新的或少见的特征）
<222> (13)..(17)
<223> n is a, c, g, or t
<220>
<221> misc_feature（新的或少见的特征）
<222> (33)..(46)
<223> n is a, c, g, or t
<220>
<221> misc_feature（新的或少见的特征）
<222> (56)..(59)
<223> n is a, c, g, or t
<400> 195
ctagtctaga gcnnnnnagt ttattcttga cannnnnnnn nnnnnntgrt ataatnnnna 60
agtaataaaa tattcggagg aattttgaaa tgaaactcgc cgtttatag 109
<210> 196
<211> 32
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> 引物_结合
<222> (1)..(32)
<223> 引物 1131
<400> 196
tcgacctcga gaagaataga ggatgaaagg tc 32
<210> 197
<211> 774
<212> DNA
<213> 布氏乳杆菌（Lactobacillus buchneri）
<220>
<221> CDS
<222> (1)..(774)
<223> 布氏乳杆菌基因编码乙偶姻还原酶
<400> 197
atg gca aag caa gtt gca tta gtt act gga gca ggt cag gga att ggt 48
Met Ala Lys Gln Val Ala Leu Val Thr Gly Ala Gly Gln Gly Ile Gly
1 5 10 15
gaa gcc atc gcg act cgt ctt cac gat gat gga ttc aat gtt gcc gtt 96
Glu Ala Ile Ala Thr Arg Leu His Asp Asp Gly Phe Asn Val Ala Val
20 25 30
gtt gat ttg aac atc gac aat gcc aac aag gta gcc aag aag ctt tca 144
Val Asp Leu Asn Ile Asp Asn Ala Asn Lys Val Ala Lys Lys Leu Ser
35 40 45
cca gat ggt tct gaa tcg att ggc atc aag gcc gac gtt tcc gac cgt 192
Pro Asp Gly Ser Glu Ser Ile Gly Ile Lys Ala Asp Val Ser Asp Arg
50 55 60
gat tca gtc atc gct gct gta aac aaa gcc gta gat gaa ttc ggc gac 240
Asp Ser Val Ile Ala Ala Val Asn Lys Ala Val Asp Glu Phe Gly Asp
65 70 75 80
ttc aac gta atc gtc aat aac gct ggt ctg ggg cca acg aca cca atc 288
Phe Asn Val Ile Val Asn Asn Ala Gly Leu Gly Pro Thr Thr Pro Ile
85 90 95
gat acg att acc ccc gat caa ttt aaa ctc gtg tat ggt gtc aac gta 336
Asp Thr Ile Thr Pro Asp Gln Phe Lys Leu Val Tyr Gly Val Asn Val
100 105 110
ggt ggt gtt ctg tgg ggc atc caa gct gca cat caa gca ttt aag aaa 384
Gly Gly Val Leu Trp Gly Ile Gln Ala Ala His Gln Ala Phe Lys Lys
115 120 125
tta ggc cat ggc ggc aag att atc aac gct act tca caa gct ggc gtt 432
Leu Gly His Gly Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala Gly Val
130 135 140
gtc ggt aat cca aac ttg gca ttg tac tca ggc acc aag ttt gcg gtt 480
Val Gly Asn Pro Asn Leu Ala Leu Tyr Ser Gly Thr Lys Phe Ala Val
145 150 155 160
cga ggc atc acc cag gtg gct gcc cgt gac ttg gca gaa gaa ggc atc 528
Arg Gly Ile Thr Gln Val Ala Ala Arg Asp Leu Ala Glu Glu Gly Ile
165 170 175
acc gca aat gct tat gca cca gga atc gta aaa aca cca atg atg ttt 576
Thr Ala Asn Ala Tyr Ala Pro Gly Ile Val Lys Thr Pro Met Met Phe
180 185 190
gat atc gcg cat caa gtc ggt aag aac gcc ggc aag gac gat gag tgg 624
Asp Ile Ala His Gln Val Gly Lys Asn Ala Gly Lys Asp Asp Glu Trp
195 200 205
ggc atg cag aca ttc gca aag gat att gcc ctc aaa cgt ttg tcg gag 672
Gly Met Gln Thr Phe Ala Lys Asp Ile Ala Leu Lys Arg Leu Ser Glu
210 215 220
ccg gaa gat gtg gca gca gct gta tca ttc ttg gca ggt aag gat tca 720
Pro Glu Asp Val Ala Ala Ala Val Ser Phe Leu Ala Gly Lys Asp Ser
225 230 235 240
aat tat gtt acc gga caa acg ctg atc gtg gat ggc gga atg caa ttt 768
Asn Tyr Val Thr Gly Gln Thr Leu Ile Val Asp Gly Gly Met Gln Phe
245 250 255
caa tag 774
Gln
<210> 198
<211> 257
<212> PRT
<213> 布氏乳杆菌（Lactobacillus buchneri）
<400> 198
Met Ala Lys Gln Val Ala Leu Val Thr Gly Ala Gly Gln Gly Ile Gly
1 5 10 15
Glu Ala Ile Ala Thr Arg Leu His Asp Asp Gly Phe Asn Val Ala Val
20 25 30
Val Asp Leu Asn Ile Asp Asn Ala Asn Lys Val Ala Lys Lys Leu Ser
35 40 45
Pro Asp Gly Ser Glu Ser Ile Gly Ile Lys Ala Asp Val Ser Asp Arg
50 55 60
Asp Ser Val Ile Ala Ala Val Asn Lys Ala Val Asp Glu Phe Gly Asp
65 70 75 80
Phe Asn Val Ile Val Asn Asn Ala Gly Leu Gly Pro Thr Thr Pro Ile
85 90 95
Asp Thr Ile Thr Pro Asp Gln Phe Lys Leu Val Tyr Gly Val Asn Val
100 105 110
Gly Gly Val Leu Trp Gly Ile Gln Ala Ala His Gln Ala Phe Lys Lys
115 120 125
Leu Gly His Gly Gly Lys Ile Ile Asn Ala Thr Ser Gln Ala Gly Val
130 135 140
Val Gly Asn Pro Asn Leu Ala Leu Tyr Ser Gly Thr Lys Phe Ala Val
145 150 155 160
Arg Gly Ile Thr Gln Val Ala Ala Arg Asp Leu Ala Glu Glu Gly Ile
165 170 175
Thr Ala Asn Ala Tyr Ala Pro Gly Ile Val Lys Thr Pro Met Met Phe
180 185 190
Asp Ile Ala His Gln Val Gly Lys Asn Ala Gly Lys Asp Asp Glu Trp
195 200 205
Gly Met Gln Thr Phe Ala Lys Asp Ile Ala Leu Lys Arg Leu Ser Glu
210 215 220
Pro Glu Asp Val Ala Ala Ala Val Ser Phe Leu Ala Gly Lys Asp Ser
225 230 235 240
Asn Tyr Val Thr Gly Gln Thr Leu Ile Val Asp Gly Gly Met Gln Phe
245 250 255
Gln
<210> 199
<211> 771
<212> DNA
<213> 布氏乳杆菌（Lactobacillus buchneri）
<220>
<221> CDS
<222> (1)..(771)
<223> 布氏乳杆菌基因编码乙偶姻还原酶
<400> 199
atg gca aaa tta gca att gta act ggc gca gga caa gga atc gga gaa 48
Met Ala Lys Leu Ala Ile Val Thr Gly Ala Gly Gln Gly Ile Gly Glu
1 5 10 15
ggc atc gct cat cga ctg gca aaa gat ggg ttc gcg att gca gtc gca 96
Gly Ile Ala His Arg Leu Ala Lys Asp Gly Phe Ala Ile Ala Val Ala
20 25 30
gat atc aat ggg gaa aca gct gat aag gta acc gca gac ttg act aac 144
Asp Ile Asn Gly Glu Thr Ala Asp Lys Val Thr Ala Asp Leu Thr Asn
35 40 45
gac ggc tac act gcc aaa tcg tat cat atc gac gtg gct gac cgc gaa 192
Asp Gly Tyr Thr Ala Lys Ser Tyr His Ile Asp Val Ala Asp Arg Glu
50 55 60
cgg gtc ttt gaa tta gtg caa aaa gcg gtt gcc gat ctt ggt gag ctt 240
Arg Val Phe Glu Leu Val Gln Lys Ala Val Ala Asp Leu Gly Glu Leu
65 70 75 80
gcc gtg tac gtc aat aat gcc ggg gtg gct ttc atc aac gaa att gtt 288
Ala Val Tyr Val Asn Asn Ala Gly Val Ala Phe Ile Asn Glu Ile Val
85 90 95
gat tcc gcg gaa aaa gac atc cgg cga tta ttg gat gtc aat tta tta 336
Asp Ser Ala Glu Lys Asp Ile Arg Arg Leu Leu Asp Val Asn Leu Leu
100 105 110
gga act tat tgg gga acc cag gcg gcg gcc gaa caa ttc aaa aaa cag 384
Gly Thr Tyr Trp Gly Thr Gln Ala Ala Ala Glu Gln Phe Lys Lys Gln
115 120 125
ggt cac ggt ggt cga atc gtt aac gcg gcc tcc ctg gcc ggt gtt gag 432
Gly His Gly Gly Arg Ile Val Asn Ala Ala Ser Leu Ala Gly Val Glu
130 135 140
gca tct gct ctg caa agc gct tat tcg att tca aag ttt ggc gtt cgg 480
Ala Ser Ala Leu Gln Ser Ala Tyr Ser Ile Ser Lys Phe Gly Val Arg
145 150 155 160
gga att act caa gca gct tcg aag gaa ttg gca aag gac caa atc acc 528
Gly Ile Thr Gln Ala Ala Ser Lys Glu Leu Ala Lys Asp Gln Ile Thr
165 170 175
gtt aac gct tac gat cca gga att gtc aga aca cct cta aga gac ggc 576
Val Asn Ala Tyr Asp Pro Gly Ile Val Arg Thr Pro Leu Arg Asp Gly
180 185 190
atc gac aaa cgg acc gct gaa atc aaa gga atc tct gtc gct gaa caa 624
Ile Asp Lys Arg Thr Ala Glu Ile Lys Gly Ile Ser Val Ala Glu Gln
195 200 205
caa aaa aac tgc ctc agt gaa atc tca ctg ggc aga gaa gcg aca cca 672
Gln Lys Asn Cys Leu Ser Glu Ile Ser Leu Gly Arg Glu Ala Thr Pro
210 215 220
gaa gac gtt gcc aaa gtc gtt tcg tgg ttt gca tcc cct gat gcc gct 720
Glu Asp Val Ala Lys Val Val Ser Trp Phe Ala Ser Pro Asp Ala Ala
225 230 235 240
tat atc acc ggc caa tca att cta gtg gat ggt ggc atg aga tat caa 768
Tyr Ile Thr Gly Gln Ser Ile Leu Val Asp Gly Gly Met Arg Tyr Gln
245 250 255
tag 771
<210> 200
<211> 256
<212> PRT
<213> 布氏乳杆菌（Lactobacillus buchneri）
<400> 200
Met Ala Lys Leu Ala Ile Val Thr Gly Ala Gly Gln Gly Ile Gly Glu
1 5 10 15
Gly Ile Ala His Arg Leu Ala Lys Asp Gly Phe Ala Ile Ala Val Ala
20 25 30
Asp Ile Asn Gly Glu Thr Ala Asp Lys Val Thr Ala Asp Leu Thr Asn
35 40 45
Asp Gly Tyr Thr Ala Lys Ser Tyr His Ile Asp Val Ala Asp Arg Glu
50 55 60
Arg Val Phe Glu Leu Val Gln Lys Ala Val Ala Asp Leu Gly Glu Leu
65 70 75 80
Ala Val Tyr Val Asn Asn Ala Gly Val Ala Phe Ile Asn Glu Ile Val
85 90 95
Asp Ser Ala Glu Lys Asp Ile Arg Arg Leu Leu Asp Val Asn Leu Leu
100 105 110
Gly Thr Tyr Trp Gly Thr Gln Ala Ala Ala Glu Gln Phe Lys Lys Gln
115 120 125
Gly His Gly Gly Arg Ile Val Asn Ala Ala Ser Leu Ala Gly Val Glu
130 135 140
Ala Ser Ala Leu Gln Ser Ala Tyr Ser Ile Ser Lys Phe Gly Val Arg
145 150 155 160
Gly Ile Thr Gln Ala Ala Ser Lys Glu Leu Ala Lys Asp Gln Ile Thr
165 170 175
Val Asn Ala Tyr Asp Pro Gly Ile Val Arg Thr Pro Leu Arg Asp Gly
180 185 190
Ile Asp Lys Arg Thr Ala Glu Ile Lys Gly Ile Ser Val Ala Glu Gln
195 200 205
Gln Lys Asn Cys Leu Ser Glu Ile Ser Leu Gly Arg Glu Ala Thr Pro
210 215 220
Glu Asp Val Ala Lys Val Val Ser Trp Phe Ala Ser Pro Asp Ala Ala
225 230 235 240
Tyr Ile Thr Gly Gln Ser Ile Leu Val Asp Gly Gly Met Arg Tyr Gln
245 250 255
<210> 201
<211> 426
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(426)
<223> LacA 基因
<400> 201
atg gct att gtt gtt ggt gca gat ctc aaa ggt acg aga tta aaa gat 48
Met Ala Ile Val Val Gly Ala Asp Leu Lys Gly Thr Arg Leu Lys Asp
1 5 10 15
gtt gtg aaa aat ttt ctt gta gaa gaa ggt ttt gag gta att gat gtg 96
Val Val Lys Asn Phe Leu Val Glu Glu Gly Phe Glu Val Ile Asp Val
20 25 30
act aag gac gga caa gat ttt gtt gat gtt acc ttg gca gtt gcc tct 144
Thr Lys Asp Gly Gln Asp Phe Val Asp Val Thr Leu Ala Val Ala Ser
35 40 45
gaa gta aat aaa gat gag caa aac tta ggt att gta att gat gct tat 192
Glu Val Asn Lys Asp Glu Gln Asn Leu Gly Ile Val Ile Asp Ala Tyr
50 55 60
gga gct ggt cca ttc atg gta gca act aaa att aaa ggt atg gtt gca 240
Gly Ala Gly Pro Phe Met Val Ala Thr Lys Ile Lys Gly Met Val Ala
65 70 75 80
gca gaa gtt tca gat gaa cgc tca gct tat atg aca cgt gga cat aac 288
Ala Glu Val Ser Asp Glu Arg Ser Ala Tyr Met Thr Arg Gly His Asn
85 90 95
aat gca cga atg att acg gtt ggc gct gaa atc gtc ggt gat gag ctt 336
Asn Ala Arg Met Ile Thr Val Gly Ala Glu Ile Val Gly Asp Glu Leu
100 105 110
gct aaa aac atc gct aaa gct ttc gtc aat ggt aaa tat gat ggc gga 384
Ala Lys Asn Ile Ala Lys Ala Phe Val Asn Gly Lys Tyr Asp Gly Gly
115 120 125
cgt cat caa gtc cga gta gat atg ctt aac aag atg tgt taa 426
Arg His Gln Val Arg Val Asp Met Leu Asn Lys Met Cys
130 135 140
<210> 202
<211> 141
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 202
Met Ala Ile Val Val Gly Ala Asp Leu Lys Gly Thr Arg Leu Lys Asp
1 5 10 15
Val Val Lys Asn Phe Leu Val Glu Glu Gly Phe Glu Val Ile Asp Val
20 25 30
Thr Lys Asp Gly Gln Asp Phe Val Asp Val Thr Leu Ala Val Ala Ser
35 40 45
Glu Val Asn Lys Asp Glu Gln Asn Leu Gly Ile Val Ile Asp Ala Tyr
50 55 60
Gly Ala Gly Pro Phe Met Val Ala Thr Lys Ile Lys Gly Met Val Ala
65 70 75 80
Ala Glu Val Ser Asp Glu Arg Ser Ala Tyr Met Thr Arg Gly His Asn
85 90 95
Asn Ala Arg Met Ile Thr Val Gly Ala Glu Ile Val Gly Asp Glu Leu
100 105 110
Ala Lys Asn Ile Ala Lys Ala Phe Val Asn Gly Lys Tyr Asp Gly Gly
115 120 125
Arg His Gln Val Arg Val Asp Met Leu Asn Lys Met Cys
130 135 140
<210> 203
<211> 516
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(516)
<223> LacB 基因
<400> 203
atg aga atc gca att gga tgt gac cac att gtc aca gat gta aaa atg 48
Met Arg Ile Ala Ile Gly Cys Asp His Ile Val Thr Asp Val Lys Met
1 5 10 15
gca gta tca gaa ttc ttg aaa tca aaa gga tat gag gtc ctt gat ttc 96
Ala Val Ser Glu Phe Leu Lys Ser Lys Gly Tyr Glu Val Leu Asp Phe
20 25 30
ggg aca tat gac cac gta cgt act cac tac cct atc tat ggt aaa aaa 144
Gly Thr Tyr Asp His Val Arg Thr His Tyr Pro Ile Tyr Gly Lys Lys
35 40 45
gtc ggt gaa gca gta gta agt ggt caa gca gac ttg gga gta tgt atc 192
Val Gly Glu Ala Val Val Ser Gly Gln Ala Asp Leu Gly Val Cys Ile
50 55 60
tgt ggt acg ggt gtc ggc att aac aat gca gtc aat aaa gtt cca ggt 240
Cys Gly Thr Gly Val Gly Ile Asn Asn Ala Val Asn Lys Val Pro Gly
65 70 75 80
gtt cgc tca gcg ttg gtt cgt gat atg acg tca gct cta tat gct aag 288
Val Arg Ser Ala Leu Val Arg Asp Met Thr Ser Ala Leu Tyr Ala Lys
85 90 95
gaa gaa ctt aat gcg aat gtt atc ggt ttt ggt gga atg att act ggt 336
Glu Glu Leu Asn Ala Asn Val Ile Gly Phe Gly Gly Met Ile Thr Gly
100 105 110
ggt ctt ctt atg aat gac atc att gaa gct ttc att gaa gct gag tac 384
Gly Leu Leu Met Asn Asp Ile Ile Glu Ala Phe Ile Glu Ala Glu Tyr
115 120 125
aaa cca aca gaa gaa aat aaa aaa ttg att gca aaa atc gaa cac gtt 432
Lys Pro Thr Glu Glu Asn Lys Lys Leu Ile Ala Lys Ile Glu His Val
130 135 140
gaa aca cat aat gca cat caa gca gat gag gaa ttc ttt aca gaa ttc 480
Glu Thr His Asn Ala His Gln Ala Asp Glu Glu Phe Phe Thr Glu Phe
145 150 155 160
ctt gaa aaa tgg gac cgt ggt gag tac cac gat taa 516
Leu Glu Lys Trp Asp Arg Gly Glu Tyr His Asp
165 170
<210> 204
<211> 171
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 204
Met Arg Ile Ala Ile Gly Cys Asp His Ile Val Thr Asp Val Lys Met
1 5 10 15
Ala Val Ser Glu Phe Leu Lys Ser Lys Gly Tyr Glu Val Leu Asp Phe
20 25 30
Gly Thr Tyr Asp His Val Arg Thr His Tyr Pro Ile Tyr Gly Lys Lys
35 40 45
Val Gly Glu Ala Val Val Ser Gly Gln Ala Asp Leu Gly Val Cys Ile
50 55 60
Cys Gly Thr Gly Val Gly Ile Asn Asn Ala Val Asn Lys Val Pro Gly
65 70 75 80
Val Arg Ser Ala Leu Val Arg Asp Met Thr Ser Ala Leu Tyr Ala Lys
85 90 95
Glu Glu Leu Asn Ala Asn Val Ile Gly Phe Gly Gly Met Ile Thr Gly
100 105 110
Gly Leu Leu Met Asn Asp Ile Ile Glu Ala Phe Ile Glu Ala Glu Tyr
115 120 125
Lys Pro Thr Glu Glu Asn Lys Lys Leu Ile Ala Lys Ile Glu His Val
130 135 140
Glu Thr His Asn Ala His Gln Ala Asp Glu Glu Phe Phe Thr Glu Phe
145 150 155 160
Leu Glu Lys Trp Asp Arg Gly Glu Tyr His Asp
165 170
<210> 205
<211> 933
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(933)
<223> LacC 基因
<400> 205
atg att ctg aca gtc aca cta aat cct tca gta gac atc tct tat cct 48
Met Ile Leu Thr Val Thr Leu Asn Pro Ser Val Asp Ile Ser Tyr Pro
1 5 10 15
cta gaa aca ttg aaa att gat aca gtg aat agg gtg aaa gat gtt agt 96
Leu Glu Thr Leu Lys Ile Asp Thr Val Asn Arg Val Lys Asp Val Ser
20 25 30
aag aca gct ggt gga aaa ggc tta aat gtt act aga gtc ttg tat gaa 144
Lys Thr Ala Gly Gly Lys Gly Leu Asn Val Thr Arg Val Leu Tyr Glu
35 40 45
tct ggt gat aaa gtt act gct aca ggt ttc tta ggt ggt aaa att ggt 192
Ser Gly Asp Lys Val Thr Ala Thr Gly Phe Leu Gly Gly Lys Ile Gly
50 55 60
gaa ttt att gaa agc gaa tta gaa caa tct cct gtt agt cca gct ttt 240
Glu Phe Ile Glu Ser Glu Leu Glu Gln Ser Pro Val Ser Pro Ala Phe
65 70 75 80
tat aaa atc tca gga aac act aga aac tgt att gct att tta cac gag 288
Tyr Lys Ile Ser Gly Asn Thr Arg Asn Cys Ile Ala Ile Leu His Glu
85 90 95
ggt aac caa aca gaa att ttg gaa caa gga cca aca att tct cat gaa 336
Gly Asn Gln Thr Glu Ile Leu Glu Gln Gly Pro Thr Ile Ser His Glu
100 105 110
gaa gca gaa ggc ttt ctt gac cac tat agc aat ctt att aag caa tca 384
Glu Ala Glu Gly Phe Leu Asp His Tyr Ser Asn Leu Ile Lys Gln Ser
115 120 125
gaa gtt gtc aca att tca gga agt ttg cct tca gga ctt ccc aac gat 432
Glu Val Val Thr Ile Ser Gly Ser Leu Pro Ser Gly Leu Pro Asn Asp
130 135 140
tat tat gaa aaa ctt atc caa ctt gct tcg gat gaa gga gta gca gtc 480
Tyr Tyr Glu Lys Leu Ile Gln Leu Ala Ser Asp Glu Gly Val Ala Val
145 150 155 160
gtt ttg gat tgt tca ggt gca cca ctt gaa aca gtt cta aaa tcg tcg 528
Val Leu Asp Cys Ser Gly Ala Pro Leu Glu Thr Val Leu Lys Ser Ser
165 170 175
gct aaa cct act gcc atc aaa cct aat aat gag gaa ctt tca cag ttg 576
Ala Lys Pro Thr Ala Ile Lys Pro Asn Asn Glu Glu Leu Ser Gln Leu
180 185 190
ctt gga aaa gaa gta aca aaa gat att gaa gaa ctc aaa gac gtt ctt 624
Leu Gly Lys Glu Val Thr Lys Asp Ile Glu Glu Leu Lys Asp Val Leu
195 200 205
aaa gag tca ctt ttt agt ggt atc gaa tgg ata gtg gtc tca tta ggt 672
Lys Glu Ser Leu Phe Ser Gly Ile Glu Trp Ile Val Val Ser Leu Gly
210 215 220
cga aac ggt gcc ttt gca aaa cat ggt gat gtt ttc tat aag gta gat 720
Arg Asn Gly Ala Phe Ala Lys His Gly Asp Val Phe Tyr Lys Val Asp
225 230 235 240
att cct gat atc cca gtt gtt aat cca gtc gga tca ggg gac tca acg 768
Ile Pro Asp Ile Pro Val Val Asn Pro Val Gly Ser Gly Asp Ser Thr
245 250 255
gta gct ggt att gca tca gct tta aat agt aaa aaa agt gac gct gac 816
Val Ala Gly Ile Ala Ser Ala Leu Asn Ser Lys Lys Ser Asp Ala Asp
260 265 270
tta tta aaa cat gcg atg aca ttg ggt atg tta aat gct caa gaa aca 864
Leu Leu Lys His Ala Met Thr Leu Gly Met Leu Asn Ala Gln Glu Thr
275 280 285
atg aca ggg cat gtg aat atg act aat tac gaa aca cta aac tca caa 912
Met Thr Gly His Val Asn Met Thr Asn Tyr Glu Thr Leu Asn Ser Gln
290 295 300
att gga gta aaa gag gta taa 933
Ile Gly Val Lys Glu Val
305 310
<210> 206
<211> 310
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 206
Met Ile Leu Thr Val Thr Leu Asn Pro Ser Val Asp Ile Ser Tyr Pro
1 5 10 15
Leu Glu Thr Leu Lys Ile Asp Thr Val Asn Arg Val Lys Asp Val Ser
20 25 30
Lys Thr Ala Gly Gly Lys Gly Leu Asn Val Thr Arg Val Leu Tyr Glu
35 40 45
Ser Gly Asp Lys Val Thr Ala Thr Gly Phe Leu Gly Gly Lys Ile Gly
50 55 60
Glu Phe Ile Glu Ser Glu Leu Glu Gln Ser Pro Val Ser Pro Ala Phe
65 70 75 80
Tyr Lys Ile Ser Gly Asn Thr Arg Asn Cys Ile Ala Ile Leu His Glu
85 90 95
Gly Asn Gln Thr Glu Ile Leu Glu Gln Gly Pro Thr Ile Ser His Glu
100 105 110
Glu Ala Glu Gly Phe Leu Asp His Tyr Ser Asn Leu Ile Lys Gln Ser
115 120 125
Glu Val Val Thr Ile Ser Gly Ser Leu Pro Ser Gly Leu Pro Asn Asp
130 135 140
Tyr Tyr Glu Lys Leu Ile Gln Leu Ala Ser Asp Glu Gly Val Ala Val
145 150 155 160
Val Leu Asp Cys Ser Gly Ala Pro Leu Glu Thr Val Leu Lys Ser Ser
165 170 175
Ala Lys Pro Thr Ala Ile Lys Pro Asn Asn Glu Glu Leu Ser Gln Leu
180 185 190
Leu Gly Lys Glu Val Thr Lys Asp Ile Glu Glu Leu Lys Asp Val Leu
195 200 205
Lys Glu Ser Leu Phe Ser Gly Ile Glu Trp Ile Val Val Ser Leu Gly
210 215 220
Arg Asn Gly Ala Phe Ala Lys His Gly Asp Val Phe Tyr Lys Val Asp
225 230 235 240
Ile Pro Asp Ile Pro Val Val Asn Pro Val Gly Ser Gly Asp Ser Thr
245 250 255
Val Ala Gly Ile Ala Ser Ala Leu Asn Ser Lys Lys Ser Asp Ala Asp
260 265 270
Leu Leu Lys His Ala Met Thr Leu Gly Met Leu Asn Ala Gln Glu Thr
275 280 285
Met Thr Gly His Val Asn Met Thr Asn Tyr Glu Thr Leu Asn Ser Gln
290 295 300
Ile Gly Val Lys Glu Val
305 310
<210> 207
<211> 981
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(981)
<223> LacD 基因
<400> 207
atg gta ctt aca gaa cag aaa cgc aaa tct ttg gaa aaa ctt tca gat 48
Met Val Leu Thr Glu Gln Lys Arg Lys Ser Leu Glu Lys Leu Ser Asp
1 5 10 15
aaa aac ggt ttt atc tca gct ttg gca ttt gac caa cgt ggt gct ttg 96
Lys Asn Gly Phe Ile Ser Ala Leu Ala Phe Asp Gln Arg Gly Ala Leu
20 25 30
aaa cgt ttg atg gca cag tat caa gat aca gaa cca act gta gct caa 144
Lys Arg Leu Met Ala Gln Tyr Gln Asp Thr Glu Pro Thr Val Ala Gln
35 40 45
atg gaa gaa cta aag gtt ttg gtt gct gat gaa ctt aca aag tac gct 192
Met Glu Glu Leu Lys Val Leu Val Ala Asp Glu Leu Thr Lys Tyr Ala
50 55 60
tct tca atg ttg ctt gac cct gaa tat gga ctt cca gca aca aaa gca 240
Ser Ser Met Leu Leu Asp Pro Glu Tyr Gly Leu Pro Ala Thr Lys Ala
65 70 75 80
ttg gat aaa gaa gca ggt ctt ctt ctt gcc tat gaa aaa aca ggt tat 288
Leu Asp Lys Glu Ala Gly Leu Leu Leu Ala Tyr Glu Lys Thr Gly Tyr
85 90 95
gat aca tca agt aca aaa cgt ttg cct gac tgc ttg gat gtt tgg tct 336
Asp Thr Ser Ser Thr Lys Arg Leu Pro Asp Cys Leu Asp Val Trp Ser
100 105 110
gca aag cgt atc aaa gag caa ggc gct gat gcc gtt aag ttc ttg ctt 384
Ala Lys Arg Ile Lys Glu Gln Gly Ala Asp Ala Val Lys Phe Leu Leu
115 120 125
tac tat gat gta gac agc tca gat gaa ctt aac caa caa aaa caa gct 432
Tyr Tyr Asp Val Asp Ser Ser Asp Glu Leu Asn Gln Gln Lys Gln Ala
130 135 140
tat att gaa cgt gtt ggt tct gaa tgt gtc gct gaa gat att cct ttc 480
Tyr Ile Glu Arg Val Gly Ser Glu Cys Val Ala Glu Asp Ile Pro Phe
145 150 155 160
ttc ttg gaa atc ctt gct tat gat gaa gaa atc tca gat gca ggt tcc 528
Phe Leu Glu Ile Leu Ala Tyr Asp Glu Glu Ile Ser Asp Ala Gly Ser
165 170 175
gtt gaa tat gca aaa gtg aaa cct cgc aaa gtc att gaa gcg atg aaa 576
Val Glu Tyr Ala Lys Val Lys Pro Arg Lys Val Ile Glu Ala Met Lys
180 185 190
gtt ttc tct gat cca cgc ttt aac att gat gtt ctt aaa gta gaa gta 624
Val Phe Ser Asp Pro Arg Phe Asn Ile Asp Val Leu Lys Val Glu Val
195 200 205
cca gtt aac gtt aaa tat gtc gaa ggt ttt gct gat ggt gaa gtg gtt 672
Pro Val Asn Val Lys Tyr Val Glu Gly Phe Ala Asp Gly Glu Val Val
210 215 220
tac agt aaa gct gaa gct gct gac ttc ttt aaa gca caa gaa gaa gcg 720
Tyr Ser Lys Ala Glu Ala Ala Asp Phe Phe Lys Ala Gln Glu Glu Ala
225 230 235 240
act aac ctt cca tac atc tac cta agt gca ggt gta tct gct aaa ttg 768
Thr Asn Leu Pro Tyr Ile Tyr Leu Ser Ala Gly Val Ser Ala Lys Leu
245 250 255
ttc caa gaa aca ttg caa ttt gct cac gac tca ggt gcc aag ttt aat 816
Phe Gln Glu Thr Leu Gln Phe Ala His Asp Ser Gly Ala Lys Phe Asn
260 265 270
ggt gtg ctt tgt gga cgt gct act tgg gca gga tct gta gaa cct tac 864
Gly Val Leu Cys Gly Arg Ala Thr Trp Ala Gly Ser Val Glu Pro Tyr
275 280 285
atc aaa gaa ggt gaa aaa gca gca cgt gaa tgg ttg cgt act act gga 912
Ile Lys Glu Gly Glu Lys Ala Ala Arg Glu Trp Leu Arg Thr Thr Gly
290 295 300
ttc gaa aat att gat gaa ctt aac aaa gtt ctt gtt aaa aca gct agt 960
Phe Glu Asn Ile Asp Glu Leu Asn Lys Val Leu Val Lys Thr Ala Ser
305 310 315 320
cca tgg act gat aaa gta tag 981
Pro Trp Thr Asp Lys Val
325
<210> 208
<211> 326
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 208
Met Val Leu Thr Glu Gln Lys Arg Lys Ser Leu Glu Lys Leu Ser Asp
1 5 10 15
Lys Asn Gly Phe Ile Ser Ala Leu Ala Phe Asp Gln Arg Gly Ala Leu
20 25 30
Lys Arg Leu Met Ala Gln Tyr Gln Asp Thr Glu Pro Thr Val Ala Gln
35 40 45
Met Glu Glu Leu Lys Val Leu Val Ala Asp Glu Leu Thr Lys Tyr Ala
50 55 60
Ser Ser Met Leu Leu Asp Pro Glu Tyr Gly Leu Pro Ala Thr Lys Ala
65 70 75 80
Leu Asp Lys Glu Ala Gly Leu Leu Leu Ala Tyr Glu Lys Thr Gly Tyr
85 90 95
Asp Thr Ser Ser Thr Lys Arg Leu Pro Asp Cys Leu Asp Val Trp Ser
100 105 110
Ala Lys Arg Ile Lys Glu Gln Gly Ala Asp Ala Val Lys Phe Leu Leu
115 120 125
Tyr Tyr Asp Val Asp Ser Ser Asp Glu Leu Asn Gln Gln Lys Gln Ala
130 135 140
Tyr Ile Glu Arg Val Gly Ser Glu Cys Val Ala Glu Asp Ile Pro Phe
145 150 155 160
Phe Leu Glu Ile Leu Ala Tyr Asp Glu Glu Ile Ser Asp Ala Gly Ser
165 170 175
Val Glu Tyr Ala Lys Val Lys Pro Arg Lys Val Ile Glu Ala Met Lys
180 185 190
Val Phe Ser Asp Pro Arg Phe Asn Ile Asp Val Leu Lys Val Glu Val
195 200 205
Pro Val Asn Val Lys Tyr Val Glu Gly Phe Ala Asp Gly Glu Val Val
210 215 220
Tyr Ser Lys Ala Glu Ala Ala Asp Phe Phe Lys Ala Gln Glu Glu Ala
225 230 235 240
Thr Asn Leu Pro Tyr Ile Tyr Leu Ser Ala Gly Val Ser Ala Lys Leu
245 250 255
Phe Gln Glu Thr Leu Gln Phe Ala His Asp Ser Gly Ala Lys Phe Asn
260 265 270
Gly Val Leu Cys Gly Arg Ala Thr Trp Ala Gly Ser Val Glu Pro Tyr
275 280 285
Ile Lys Glu Gly Glu Lys Ala Ala Arg Glu Trp Leu Arg Thr Thr Gly
290 295 300
Phe Glu Asn Ile Asp Glu Leu Asn Lys Val Leu Val Lys Thr Ala Ser
305 310 315 320
Pro Trp Thr Asp Lys Val
325
<210> 209
<211> 1707
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(1707)
<223> LacE 基因
<400> 209
atg cat aaa ctc att gaa ctt att gag aaa ggg aaa cca ttc ttt gag 48
Met His Lys Leu Ile Glu Leu Ile Glu Lys Gly Lys Pro Phe Phe Glu
1 5 10 15
aaa atc tct cga aac atc tat ctt cgt gct att cgt gat gga ttt att 96
Lys Ile Ser Arg Asn Ile Tyr Leu Arg Ala Ile Arg Asp Gly Phe Ile
20 25 30
gct ggt atg cca gtc atc ctt ttc tca tct atc ttt atc ctt att gcc 144
Ala Gly Met Pro Val Ile Leu Phe Ser Ser Ile Phe Ile Leu Ile Ala
35 40 45
tat gtc cca aat gct tgg gga ttc cac tgg tca aaa gac att gag acc 192
Tyr Val Pro Asn Ala Trp Gly Phe His Trp Ser Lys Asp Ile Glu Thr
50 55 60
ttc ttg atg act cct tat agc tat tcg atg ggt atc cta gca ttc ttt 240
Phe Leu Met Thr Pro Tyr Ser Tyr Ser Met Gly Ile Leu Ala Phe Phe
65 70 75 80
gtt ggt ggt act acc gct aaa gct ttg acg gac tca aag aac cgt gac 288
Val Gly Gly Thr Thr Ala Lys Ala Leu Thr Asp Ser Lys Asn Arg Asp
85 90 95
cta cct gcg acc aat cag att aac ttc tta tct acg atg cta gcg tct 336
Leu Pro Ala Thr Asn Gln Ile Asn Phe Leu Ser Thr Met Leu Ala Ser
100 105 110
atg gta ggg ttc ctt ttg atg gca gct gag cct gca aaa gaa gga ggc 384
Met Val Gly Phe Leu Leu Met Ala Ala Glu Pro Ala Lys Glu Gly Gly
115 120 125
ttc ttg aca gcc ttc atg gga aca aaa ggc ctt ttg aca gca ttt atc 432
Phe Leu Thr Ala Phe Met Gly Thr Lys Gly Leu Leu Thr Ala Phe Ile
130 135 140
gca gcc ttt gtg aca gtt aat gtt tat aaa gtc tgt gta aaa aat aat 480
Ala Ala Phe Val Thr Val Asn Val Tyr Lys Val Cys Val Lys Asn Asn
145 150 155 160
gtt acc att cgt atg cct gaa gat gtt cca cca aat atc tct caa gta 528
Val Thr Ile Arg Met Pro Glu Asp Val Pro Pro Asn Ile Ser Gln Val
165 170 175
ttt aag gac ttg att ccg ttc acc gta tca gtt gtt ctc cta tat gga 576
Phe Lys Asp Leu Ile Pro Phe Thr Val Ser Val Val Leu Leu Tyr Gly
180 185 190
ctt gaa ctc ctt gtt aag gga act ctt ggt gta act gtt gca gaa tca 624
Leu Glu Leu Leu Val Lys Gly Thr Leu Gly Val Thr Val Ala Glu Ser
195 200 205
atc ggt acc ctt att gct cct ctt ttc tca gct gca gat ggt tac ctt 672
Ile Gly Thr Leu Ile Ala Pro Leu Phe Ser Ala Ala Asp Gly Tyr Leu
210 215 220
gga att aca ctt atc ttt ggt gct tat gcc ttc ttc tgg ttt gtt ggt 720
Gly Ile Thr Leu Ile Phe Gly Ala Tyr Ala Phe Phe Trp Phe Val Gly
225 230 235 240
att cac ggt cct tca att gtc gaa cca gca atc gct gct atc act tat 768
Ile His Gly Pro Ser Ile Val Glu Pro Ala Ile Ala Ala Ile Thr Tyr
245 250 255
gcc aat atc gat gtc aac ttg cat ctt atc caa gct gga caa cat gca 816
Ala Asn Ile Asp Val Asn Leu His Leu Ile Gln Ala Gly Gln His Ala
260 265 270
gat aaa gtt atc act tct ggt act caa atg ttt att gct acc atg ggt 864
Asp Lys Val Ile Thr Ser Gly Thr Gln Met Phe Ile Ala Thr Met Gly
275 280 285
gga aca gga gct aca ttg att gtt cca ttc ttg ttc atg tgg att tgt 912
Gly Thr Gly Ala Thr Leu Ile Val Pro Phe Leu Phe Met Trp Ile Cys
290 295 300
aaa tca gat cgt aac cgt gcc atc gga cgt gcc tca gtt gtt cca aca 960
Lys Ser Asp Arg Asn Arg Ala Ile Gly Arg Ala Ser Val Val Pro Thr
305 310 315 320
ttc ttt ggg gtt aat gag cca atc tta ttt ggt gca cca atc gta ttg 1008
Phe Phe Gly Val Asn Glu Pro Ile Leu Phe Gly Ala Pro Ile Val Leu
325 330 335
aat ccg att ttc ttt gta ccg ttc att ttc gct ccg att gtc aac gtt 1056
Asn Pro Ile Phe Phe Val Pro Phe Ile Phe Ala Pro Ile Val Asn Val
340 345 350
tgg atc ttt aaa ttc ttt gtt gac aca ttg aac atg aac tca ttc tct 1104
Trp Ile Phe Lys Phe Phe Val Asp Thr Leu Asn Met Asn Ser Phe Ser
355 360 365
gcc aac ctt cca tgg gtt act cct ggt ccg ttg gga att gta ctc ggt 1152
Ala Asn Leu Pro Trp Val Thr Pro Gly Pro Leu Gly Ile Val Leu Gly
370 375 380
act aac ttc caa gtt cta tca ttt atc tta gcc gga ctc ttg gta gtt 1200
Thr Asn Phe Gln Val Leu Ser Phe Ile Leu Ala Gly Leu Leu Val Val
385 390 395 400
gtt gat act atc att tat tac cca ttt gtt aag gta tac gat gaa caa 1248
Val Asp Thr Ile Ile Tyr Tyr Pro Phe Val Lys Val Tyr Asp Glu Gln
405 410 415
att ctt gaa gaa gaa cgt tct ggt aaa aca aac gat gct ctt aaa gaa 1296
Ile Leu Glu Glu Glu Arg Ser Gly Lys Thr Asn Asp Ala Leu Lys Glu
420 425 430
aaa gta gct gca aac ttc aat act gct aaa gca gat gcc gtt ctt gga 1344
Lys Val Ala Ala Asn Phe Asn Thr Ala Lys Ala Asp Ala Val Leu Gly
435 440 445
aaa gca gac gtt gct aaa gaa gat gta gca gca aac aat aat atc aca 1392
Lys Ala Asp Val Ala Lys Glu Asp Val Ala Ala Asn Asn Asn Ile Thr
450 455 460
aaa gaa act aat gtc ctt gtt ctt tgt gca ggt gga ggt aca agt ggc 1440
Lys Glu Thr Asn Val Leu Val Leu Cys Ala Gly Gly Gly Thr Ser Gly
465 470 475 480
ttg ctt gcc aat gcc ttg aac aaa gca gca gca gaa tac aat gtt cca 1488
Leu Leu Ala Asn Ala Leu Asn Lys Ala Ala Ala Glu Tyr Asn Val Pro
485 490 495
gtc aag gcg gca gcg ggt ggt tac ggt gct cac cgt gaa atg ttg cca 1536
Val Lys Ala Ala Ala Gly Gly Tyr Gly Ala His Arg Glu Met Leu Pro
500 505 510
gag ttt gac ttg gta atc ttg gct cca cag gtc gct tca aac ttc gat 1584
Glu Phe Asp Leu Val Ile Leu Ala Pro Gln Val Ala Ser Asn Phe Asp
515 520 525
gat atg aag gct gaa aca gat aaa ttg ggc att aaa ctt gta aaa act 1632
Asp Met Lys Ala Glu Thr Asp Lys Leu Gly Ile Lys Leu Val Lys Thr
530 535 540
gaa gga gct caa tat atc aaa ttg aca cgt gac gga caa ggt gct ctt 1680
Glu Gly Ala Gln Tyr Ile Lys Leu Thr Arg Asp Gly Gln Gly Ala Leu
545 550 555 560
gca ttt gtt caa caa cag ttt gat taa 1707
Ala Phe Val Gln Gln Gln Phe Asp
565
<210> 210
<211> 568
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 210
Met His Lys Leu Ile Glu Leu Ile Glu Lys Gly Lys Pro Phe Phe Glu
1 5 10 15
Lys Ile Ser Arg Asn Ile Tyr Leu Arg Ala Ile Arg Asp Gly Phe Ile
20 25 30
Ala Gly Met Pro Val Ile Leu Phe Ser Ser Ile Phe Ile Leu Ile Ala
35 40 45
Tyr Val Pro Asn Ala Trp Gly Phe His Trp Ser Lys Asp Ile Glu Thr
50 55 60
Phe Leu Met Thr Pro Tyr Ser Tyr Ser Met Gly Ile Leu Ala Phe Phe
65 70 75 80
Val Gly Gly Thr Thr Ala Lys Ala Leu Thr Asp Ser Lys Asn Arg Asp
85 90 95
Leu Pro Ala Thr Asn Gln Ile Asn Phe Leu Ser Thr Met Leu Ala Ser
100 105 110
Met Val Gly Phe Leu Leu Met Ala Ala Glu Pro Ala Lys Glu Gly Gly
115 120 125
Phe Leu Thr Ala Phe Met Gly Thr Lys Gly Leu Leu Thr Ala Phe Ile
130 135 140
Ala Ala Phe Val Thr Val Asn Val Tyr Lys Val Cys Val Lys Asn Asn
145 150 155 160
Val Thr Ile Arg Met Pro Glu Asp Val Pro Pro Asn Ile Ser Gln Val
165 170 175
Phe Lys Asp Leu Ile Pro Phe Thr Val Ser Val Val Leu Leu Tyr Gly
180 185 190
Leu Glu Leu Leu Val Lys Gly Thr Leu Gly Val Thr Val Ala Glu Ser
195 200 205
Ile Gly Thr Leu Ile Ala Pro Leu Phe Ser Ala Ala Asp Gly Tyr Leu
210 215 220
Gly Ile Thr Leu Ile Phe Gly Ala Tyr Ala Phe Phe Trp Phe Val Gly
225 230 235 240
Ile His Gly Pro Ser Ile Val Glu Pro Ala Ile Ala Ala Ile Thr Tyr
245 250 255
Ala Asn Ile Asp Val Asn Leu His Leu Ile Gln Ala Gly Gln His Ala
260 265 270
Asp Lys Val Ile Thr Ser Gly Thr Gln Met Phe Ile Ala Thr Met Gly
275 280 285
Gly Thr Gly Ala Thr Leu Ile Val Pro Phe Leu Phe Met Trp Ile Cys
290 295 300
Lys Ser Asp Arg Asn Arg Ala Ile Gly Arg Ala Ser Val Val Pro Thr
305 310 315 320
Phe Phe Gly Val Asn Glu Pro Ile Leu Phe Gly Ala Pro Ile Val Leu
325 330 335
Asn Pro Ile Phe Phe Val Pro Phe Ile Phe Ala Pro Ile Val Asn Val
340 345 350
Trp Ile Phe Lys Phe Phe Val Asp Thr Leu Asn Met Asn Ser Phe Ser
355 360 365
Ala Asn Leu Pro Trp Val Thr Pro Gly Pro Leu Gly Ile Val Leu Gly
370 375 380
Thr Asn Phe Gln Val Leu Ser Phe Ile Leu Ala Gly Leu Leu Val Val
385 390 395 400
Val Asp Thr Ile Ile Tyr Tyr Pro Phe Val Lys Val Tyr Asp Glu Gln
405 410 415
Ile Leu Glu Glu Glu Arg Ser Gly Lys Thr Asn Asp Ala Leu Lys Glu
420 425 430
Lys Val Ala Ala Asn Phe Asn Thr Ala Lys Ala Asp Ala Val Leu Gly
435 440 445
Lys Ala Asp Val Ala Lys Glu Asp Val Ala Ala Asn Asn Asn Ile Thr
450 455 460
Lys Glu Thr Asn Val Leu Val Leu Cys Ala Gly Gly Gly Thr Ser Gly
465 470 475 480
Leu Leu Ala Asn Ala Leu Asn Lys Ala Ala Ala Glu Tyr Asn Val Pro
485 490 495
Val Lys Ala Ala Ala Gly Gly Tyr Gly Ala His Arg Glu Met Leu Pro
500 505 510
Glu Phe Asp Leu Val Ile Leu Ala Pro Gln Val Ala Ser Asn Phe Asp
515 520 525
Asp Met Lys Ala Glu Thr Asp Lys Leu Gly Ile Lys Leu Val Lys Thr
530 535 540
Glu Gly Ala Gln Tyr Ile Lys Leu Thr Arg Asp Gly Gln Gly Ala Leu
545 550 555 560
Ala Phe Val Gln Gln Gln Phe Asp
565
<210> 211
<211> 318
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(318)
<223> LacF 基因
<400> 211
atg aac aga gaa gag atg act ctc tta ggg ttt gaa att gtt gct tat 48
Met Asn Arg Glu Glu Met Thr Leu Leu Gly Phe Glu Ile Val Ala Tyr
1 5 10 15
gct gga gat gct cgc tct aag ctt tta gaa gcg ctt aaa gcg gct gaa 96
Ala Gly Asp Ala Arg Ser Lys Leu Leu Glu Ala Leu Lys Ala Ala Glu
20 25 30
aat ggt gat ttc gct aag gca gat agt ctt gta gta gaa gca gga agc 144
Asn Gly Asp Phe Ala Lys Ala Asp Ser Leu Val Val Glu Ala Gly Ser
35 40 45
tgt att gca gag gct cac agt tct cag aca ggt atg ttg gct cga gaa 192
Cys Ile Ala Glu Ala His Ser Ser Gln Thr Gly Met Leu Ala Arg Glu
50 55 60
gct tct ggg gag gaa ctt cca tac agt gtt act atg atg cat ggt cag 240
Ala Ser Gly Glu Glu Leu Pro Tyr Ser Val Thr Met Met His Gly Gln
65 70 75 80
gat cac ttg atg act acg atc tta tta aaa gat gtg att cat cac ctc 288
Asp His Leu Met Thr Thr Ile Leu Leu Lys Asp Val Ile His His Leu
85 90 95
atc gaa ctt tat aaa aga gga gca aag taa 318
Ile Glu Leu Tyr Lys Arg Gly Ala Lys
100 105
<210> 212
<211> 105
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 212
Met Asn Arg Glu Glu Met Thr Leu Leu Gly Phe Glu Ile Val Ala Tyr
1 5 10 15
Ala Gly Asp Ala Arg Ser Lys Leu Leu Glu Ala Leu Lys Ala Ala Glu
20 25 30
Asn Gly Asp Phe Ala Lys Ala Asp Ser Leu Val Val Glu Ala Gly Ser
35 40 45
Cys Ile Ala Glu Ala His Ser Ser Gln Thr Gly Met Leu Ala Arg Glu
50 55 60
Ala Ser Gly Glu Glu Leu Pro Tyr Ser Val Thr Met Met His Gly Gln
65 70 75 80
Asp His Leu Met Thr Thr Ile Leu Leu Lys Asp Val Ile His His Leu
85 90 95
Ile Glu Leu Tyr Lys Arg Gly Ala Lys
100 105
<210> 213
<211> 1407
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(1407)
<223> LacG 基因
<400> 213
atg act aaa aca ctt cct aaa gat ttt att ttt ggt ggt gct aca gct 48
Met Thr Lys Thr Leu Pro Lys Asp Phe Ile Phe Gly Gly Ala Thr Ala
1 5 10 15
gct tat caa gca gaa ggt gca act cac aca gat ggt aag ggc cca gta 96
Ala Tyr Gln Ala Glu Gly Ala Thr His Thr Asp Gly Lys Gly Pro Val
20 25 30
gca tgg gat aag tat ctt gaa gac aat tat tgg tat aca gca gaa cct 144
Ala Trp Asp Lys Tyr Leu Glu Asp Asn Tyr Trp Tyr Thr Ala Glu Pro
35 40 45
gca agt gat ttt tac cat aaa tac cca gtt gat tta gaa tta gct gaa 192
Ala Ser Asp Phe Tyr His Lys Tyr Pro Val Asp Leu Glu Leu Ala Glu
50 55 60
gaa tat ggt gtt aat ggt att cgt att tct att gcc tgg tct cgt att 240
Glu Tyr Gly Val Asn Gly Ile Arg Ile Ser Ile Ala Trp Ser Arg Ile
65 70 75 80
ttc cca acg ggt tac ggt gaa gta aat gaa aaa ggt gtt gaa ttc tac 288
Phe Pro Thr Gly Tyr Gly Glu Val Asn Glu Lys Gly Val Glu Phe Tyr
85 90 95
cat aaa cta ttt gct gaa tgc cac aag cgt cat gtt gaa cca ttt gtg 336
His Lys Leu Phe Ala Glu Cys His Lys Arg His Val Glu Pro Phe Val
100 105 110
act ttg cat cat ttt gac aca cca gaa gct ctt cat tca aat gga gat 384
Thr Leu His His Phe Asp Thr Pro Glu Ala Leu His Ser Asn Gly Asp
115 120 125
ttc tta aac cgc gaa aat ata gaa cac ttt ata gat tat gcc gct ttc 432
Phe Leu Asn Arg Glu Asn Ile Glu His Phe Ile Asp Tyr Ala Ala Phe
130 135 140
tgt ttt gaa gag ttt cca gaa gta aac tac tgg aca act ttc aat gaa 480
Cys Phe Glu Glu Phe Pro Glu Val Asn Tyr Trp Thr Thr Phe Asn Glu
145 150 155 160
att ggc cca att ggt gat ggt caa tac ttg gtt ggt aaa ttc cct cca 528
Ile Gly Pro Ile Gly Asp Gly Gln Tyr Leu Val Gly Lys Phe Pro Pro
165 170 175
ggc att aaa tat gat ctt gcc aaa gtc ttc caa tca cac cac aac atg 576
Gly Ile Lys Tyr Asp Leu Ala Lys Val Phe Gln Ser His His Asn Met
180 185 190
atg gtc tct cat gca cgt gct gta aaa ttg tat aag gat aaa ggt tat 624
Met Val Ser His Ala Arg Ala Val Lys Leu Tyr Lys Asp Lys Gly Tyr
195 200 205
aaa ggt gaa att ggt gtt gtt cat gct tta cca act aaa tac cct tat 672
Lys Gly Glu Ile Gly Val Val His Ala Leu Pro Thr Lys Tyr Pro Tyr
210 215 220
gat ccg gaa aat cca gca gat gtt cgt gct gct gaa ctt gaa gac atc 720
Asp Pro Glu Asn Pro Ala Asp Val Arg Ala Ala Glu Leu Glu Asp Ile
225 230 235 240
atc cat aac aaa ttt atc ttg gat gca acc tat ctt ggg cac tat tca 768
Ile His Asn Lys Phe Ile Leu Asp Ala Thr Tyr Leu Gly His Tyr Ser
245 250 255
gat aaa acg atg gaa ggt gtc aac cat atc cta gct gag aat ggt gga 816
Asp Lys Thr Met Glu Gly Val Asn His Ile Leu Ala Glu Asn Gly Gly
260 265 270
gaa ctt gat ctt cgt gat gaa gac ttc caa gct ctt gac gca gct aaa 864
Glu Leu Asp Leu Arg Asp Glu Asp Phe Gln Ala Leu Asp Ala Ala Lys
275 280 285
gat ttg aat gat ttc ctt ggt atc aac tat tac atg agt gat tgg atg 912
Asp Leu Asn Asp Phe Leu Gly Ile Asn Tyr Tyr Met Ser Asp Trp Met
290 295 300
caa gct ttt gat ggt gag act gaa atc att cac aat ggt aag ggt gaa 960
Gln Ala Phe Asp Gly Glu Thr Glu Ile Ile His Asn Gly Lys Gly Glu
305 310 315 320
aaa gga agc tct aag tac caa att aag ggt gtt ggt cgt cga gta gct 1008
Lys Gly Ser Ser Lys Tyr Gln Ile Lys Gly Val Gly Arg Arg Val Ala
325 330 335
cct gac tat gtt ccg cgc aca gac tgg gat tgg att att tat cct gaa 1056
Pro Asp Tyr Val Pro Arg Thr Asp Trp Asp Trp Ile Ile Tyr Pro Glu
340 345 350
ggc ttg tat gac caa atc atg cga gtg aaa aat gat tat ccg aat tac 1104
Gly Leu Tyr Asp Gln Ile Met Arg Val Lys Asn Asp Tyr Pro Asn Tyr
355 360 365
aag aag att tac atc act gaa aac ggt ctc gga tac aaa gat gag ttt 1152
Lys Lys Ile Tyr Ile Thr Glu Asn Gly Leu Gly Tyr Lys Asp Glu Phe
370 375 380
gta gat aat act gtt tat gat gat ggt cgt att gat tac gtg aag caa 1200
Val Asp Asn Thr Val Tyr Asp Asp Gly Arg Ile Asp Tyr Val Lys Gln
385 390 395 400
cac ttg gaa gtt tta tca gac gcg att gcg gat ggt gca aat gtt aaa 1248
His Leu Glu Val Leu Ser Asp Ala Ile Ala Asp Gly Ala Asn Val Lys
405 410 415
ggt tac ttc att tgg tca ctt atg gac gtt ttc tca tgg tca aat ggt 1296
Gly Tyr Phe Ile Trp Ser Leu Met Asp Val Phe Ser Trp Ser Asn Gly
420 425 430
tat gaa aaa cgt tat gga ttg ttc tat gta gac ttt gat acg caa gaa 1344
Tyr Glu Lys Arg Tyr Gly Leu Phe Tyr Val Asp Phe Asp Thr Gln Glu
435 440 445
cgc tat cct aag aaa tca gca cat tgg tat aag aaa tta gca gaa act 1392
Arg Tyr Pro Lys Lys Ser Ala His Trp Tyr Lys Lys Leu Ala Glu Thr
450 455 460
caa gtg ata gag taa 1407
Gln Val Ile Glu
465
<210> 214
<211> 468
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 214
Met Thr Lys Thr Leu Pro Lys Asp Phe Ile Phe Gly Gly Ala Thr Ala
1 5 10 15
Ala Tyr Gln Ala Glu Gly Ala Thr His Thr Asp Gly Lys Gly Pro Val
20 25 30
Ala Trp Asp Lys Tyr Leu Glu Asp Asn Tyr Trp Tyr Thr Ala Glu Pro
35 40 45
Ala Ser Asp Phe Tyr His Lys Tyr Pro Val Asp Leu Glu Leu Ala Glu
50 55 60
Glu Tyr Gly Val Asn Gly Ile Arg Ile Ser Ile Ala Trp Ser Arg Ile
65 70 75 80
Phe Pro Thr Gly Tyr Gly Glu Val Asn Glu Lys Gly Val Glu Phe Tyr
85 90 95
His Lys Leu Phe Ala Glu Cys His Lys Arg His Val Glu Pro Phe Val
100 105 110
Thr Leu His His Phe Asp Thr Pro Glu Ala Leu His Ser Asn Gly Asp
115 120 125
Phe Leu Asn Arg Glu Asn Ile Glu His Phe Ile Asp Tyr Ala Ala Phe
130 135 140
Cys Phe Glu Glu Phe Pro Glu Val Asn Tyr Trp Thr Thr Phe Asn Glu
145 150 155 160
Ile Gly Pro Ile Gly Asp Gly Gln Tyr Leu Val Gly Lys Phe Pro Pro
165 170 175
Gly Ile Lys Tyr Asp Leu Ala Lys Val Phe Gln Ser His His Asn Met
180 185 190
Met Val Ser His Ala Arg Ala Val Lys Leu Tyr Lys Asp Lys Gly Tyr
195 200 205
Lys Gly Glu Ile Gly Val Val His Ala Leu Pro Thr Lys Tyr Pro Tyr
210 215 220
Asp Pro Glu Asn Pro Ala Asp Val Arg Ala Ala Glu Leu Glu Asp Ile
225 230 235 240
Ile His Asn Lys Phe Ile Leu Asp Ala Thr Tyr Leu Gly His Tyr Ser
245 250 255
Asp Lys Thr Met Glu Gly Val Asn His Ile Leu Ala Glu Asn Gly Gly
260 265 270
Glu Leu Asp Leu Arg Asp Glu Asp Phe Gln Ala Leu Asp Ala Ala Lys
275 280 285
Asp Leu Asn Asp Phe Leu Gly Ile Asn Tyr Tyr Met Ser Asp Trp Met
290 295 300
Gln Ala Phe Asp Gly Glu Thr Glu Ile Ile His Asn Gly Lys Gly Glu
305 310 315 320
Lys Gly Ser Ser Lys Tyr Gln Ile Lys Gly Val Gly Arg Arg Val Ala
325 330 335
Pro Asp Tyr Val Pro Arg Thr Asp Trp Asp Trp Ile Ile Tyr Pro Glu
340 345 350
Gly Leu Tyr Asp Gln Ile Met Arg Val Lys Asn Asp Tyr Pro Asn Tyr
355 360 365
Lys Lys Ile Tyr Ile Thr Glu Asn Gly Leu Gly Tyr Lys Asp Glu Phe
370 375 380
Val Asp Asn Thr Val Tyr Asp Asp Gly Arg Ile Asp Tyr Val Lys Gln
385 390 395 400
His Leu Glu Val Leu Ser Asp Ala Ile Ala Asp Gly Ala Asn Val Lys
405 410 415
Gly Tyr Phe Ile Trp Ser Leu Met Asp Val Phe Ser Trp Ser Asn Gly
420 425 430
Tyr Glu Lys Arg Tyr Gly Leu Phe Tyr Val Asp Phe Asp Thr Gln Glu
435 440 445
Arg Tyr Pro Lys Lys Ser Ala His Trp Tyr Lys Lys Leu Ala Glu Thr
450 455 460
Gln Val Ile Glu
465
<210> 215
<211> 768
<212> DNA
<213> 乳酸乳球菌（Lactococcus lactis）
<220>
<221> CDS
<222> (1)..(768)
<223> LacR 基因
<400> 215
atg aac aaa aaa cga cga tta gaa aaa att tta gat atg tta aag att 48
Met Asn Lys Lys Arg Arg Leu Glu Lys Ile Leu Asp Met Leu Lys Ile
1 5 10 15
gat ggg acc ata acc ata aaa gaa ata ata gat gaa cta gat att tcc 96
Asp Gly Thr Ile Thr Ile Lys Glu Ile Ile Asp Glu Leu Asp Ile Ser
20 25 30
gat atg aca gcc cgt aga gac ctt gat gct cta gaa gct gat gga ctt 144
Asp Met Thr Ala Arg Arg Asp Leu Asp Ala Leu Glu Ala Asp Gly Leu
35 40 45
tta aca cgt act cat ggt ggt gca caa ttg ctt tcc tct aaa aag cca 192
Leu Thr Arg Thr His Gly Gly Ala Gln Leu Leu Ser Ser Lys Lys Pro
50 55 60
ctt gaa aag aca cat atc gag aag aaa agt cta aat aca aaa gaa aaa 240
Leu Glu Lys Thr His Ile Glu Lys Lys Ser Leu Asn Thr Lys Glu Lys
65 70 75 80
att gac att gct aaa aaa gcc tgc tct tta atc aaa gat ggc gat act 288
Ile Asp Ile Ala Lys Lys Ala Cys Ser Leu Ile Lys Asp Gly Asp Thr
85 90 95
att ttt att gga ccc gga act aca ctt gta caa ctg gca tta gaa ttg 336
Ile Phe Ile Gly Pro Gly Thr Thr Leu Val Gln Leu Ala Leu Glu Leu
100 105 110
aaa ggt cgt aaa ggt tat aaa att cgt gtc att aca aat agt ctc cct 384
Lys Gly Arg Lys Gly Tyr Lys Ile Arg Val Ile Thr Asn Ser Leu Pro
115 120 125
gtg ttc ttg att cta aat gat agc gaa acc att gat tta ttg ctt ctt 432
Val Phe Leu Ile Leu Asn Asp Ser Glu Thr Ile Asp Leu Leu Leu Leu
130 135 140
ggc ggt gaa tat aga gaa ata act gga gct ttt gta ggt tca atg gct 480
Gly Gly Glu Tyr Arg Glu Ile Thr Gly Ala Phe Val Gly Ser Met Ala
145 150 155 160
tcg aca aat tta aaa gca atg aga ttt gcc aaa gct ttt gtt agt gca 528
Ser Thr Asn Leu Lys Ala Met Arg Phe Ala Lys Ala Phe Val Ser Ala
165 170 175
aat gct gtt acc cat aat tct att gct aca tat agt gac aag gaa ggt 576
Asn Ala Val Thr His Asn Ser Ile Ala Thr Tyr Ser Asp Lys Glu Gly
180 185 190
gtg att caa caa ctt gcc cta aac aat gct gta gaa aaa ttc tta tta 624
Val Ile Gln Gln Leu Ala Leu Asn Asn Ala Val Glu Lys Phe Leu Leu
195 200 205
gta gac agt act aaa ttc gat cga tac gat ttc ttt aac ttc tac aat 672
Val Asp Ser Thr Lys Phe Asp Arg Tyr Asp Phe Phe Asn Phe Tyr Asn
210 215 220
cta gat caa ctc gat acc atc att aca gat aac cag att agc cct caa 720
Leu Asp Gln Leu Asp Thr Ile Ile Thr Asp Asn Gln Ile Ser Pro Gln
225 230 235 240
cac tta gag gaa ttt agc cag tac act act att tta aaa gcg gac tag 768
His Leu Glu Glu Phe Ser Gln Tyr Thr Thr Ile Leu Lys Ala Asp
245 250 255
<210> 216
<211> 255
<212> PRT
<213> 乳酸乳球菌（Lactococcus lactis）
<400> 216
Met Asn Lys Lys Arg Arg Leu Glu Lys Ile Leu Asp Met Leu Lys Ile
1 5 10 15
Asp Gly Thr Ile Thr Ile Lys Glu Ile Ile Asp Glu Leu Asp Ile Ser
20 25 30
Asp Met Thr Ala Arg Arg Asp Leu Asp Ala Leu Glu Ala Asp Gly Leu
35 40 45
Leu Thr Arg Thr His Gly Gly Ala Gln Leu Leu Ser Ser Lys Lys Pro
50 55 60
Leu Glu Lys Thr His Ile Glu Lys Lys Ser Leu Asn Thr Lys Glu Lys
65 70 75 80
Ile Asp Ile Ala Lys Lys Ala Cys Ser Leu Ile Lys Asp Gly Asp Thr
85 90 95
Ile Phe Ile Gly Pro Gly Thr Thr Leu Val Gln Leu Ala Leu Glu Leu
100 105 110
Lys Gly Arg Lys Gly Tyr Lys Ile Arg Val Ile Thr Asn Ser Leu Pro
115 120 125
Val Phe Leu Ile Leu Asn Asp Ser Glu Thr Ile Asp Leu Leu Leu Leu
130 135 140
Gly Gly Glu Tyr Arg Glu Ile Thr Gly Ala Phe Val Gly Ser Met Ala
145 150 155 160
Ser Thr Asn Leu Lys Ala Met Arg Phe Ala Lys Ala Phe Val Ser Ala
165 170 175
Asn Ala Val Thr His Asn Ser Ile Ala Thr Tyr Ser Asp Lys Glu Gly
180 185 190
Val Ile Gln Gln Leu Ala Leu Asn Asn Ala Val Glu Lys Phe Leu Leu
195 200 205
Val Asp Ser Thr Lys Phe Asp Arg Tyr Asp Phe Phe Asn Phe Tyr Asn
210 215 220
Leu Asp Gln Leu Asp Thr Ile Ile Thr Asp Asn Gln Ile Ser Pro Gln
225 230 235 240
His Leu Glu Glu Phe Ser Gln Tyr Thr Thr Ile Leu Lys Ala Asp
245 250 255

Claims (16)

1.一种用于生产双乙酰的基因修饰的乳酸菌，其中，所述乳酸菌的基因组缺失基因或缺少基因，所述基因编码具有以下酶活性的多肽：
a.乳酸脱氢酶(E.C.1.1.1.27或E.C.1.1.1.28)
b.α-乙酰乳酸脱羧酶(E.C.4.1.1.5)
c.磷酸转乙酰酶(E.C.2.3.1.8)和
d.NADH氧化酶(E.C.1.6.3.4)；和
其中所述微生物缺乏编码具有以下酶活性的多肽的转基因：
e.双乙酰还原酶(E.C.1.1.1.304)和
f.L-丁二醇脱氢酶(E.C.1.1.1.76)
2.根据权利要求1所述的基因修饰的乳酸菌，其中，所述乳酸菌的基因组另外缺失一种或多种基因或者缺少一种或多种基因，所述基因编码具有选自以下组的酶活性的多肽：
g.乙醇脱氢酶(E.C.1.2.1.10)
h.双乙酰还原酶(E.C.1.1.1.303)
i.丁二醇脱氢酶(E.C.1.1.1.4)和
j.D-乙偶姻还原酶。
3.根据权利要求1或2所述的基因修饰的乳酸菌，其中，所述乳酸菌属于选自由以下组成的组的属：乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、片球菌属(Pediococcus)、明串珠菌属(Leuconostoc)，链球菌属(Streptococcus)、酒球菌属(Oenococcus)和芽孢杆菌属(Bacillus)。
4.根据权利要求1-3中任一项所述的基因修饰的乳酸菌，其中：
a.具有乳酸脱氢酶活性的所述多肽的氨基酸序列具有与选自以下中的氨基酸序列至少80％的序列同一性：SEQ ID NO：2、4、6、8、10、12、14、16、18、22、24、26、28、30、32、34和36。
5.根据权利要求1-4中任一项所述的基因修饰的乳酸菌，其中：
a.具有磷酸转乙酰酶活性的所述多肽的氨基酸序列具有与选自以下中的氨基酸序列至少80％的序列同一性：SEQ ID NO：42、44、46、48、50、52、54、56、58和60。
6.根据权利要求1-5中任一项所述的基因修饰的乳酸菌，其中，具有α-乙酰乳酸脱羧酶活性的所述多肽的氨基酸序列具有与选自以下中的氨基酸序列至少80％的序列同一性：SEQ ID NO：80、82、84、86、88、90、92、94和96。
7.根据权利要求2-6中任一项所述的基因修饰的乳酸菌，其中：
a.具有乙醇脱氢酶活性的所述多肽的氨基酸序列具有与选自以下中的氨基酸序列至少80％的序列同一性：SEQ ID NO：62、64、66、68、70、72、74、76和78；
b.具有双乙酰还原酶(E.C.1.1.1.303)活性的所述多肽的氨基酸序列具有与SEQ IDNO：98至少80％的序列同一性；
c.具有D-乙偶姻还原酶活性的所述多肽的氨基酸序列具有与SEQ ID NO：100、102、104、106、108、110和112至少80％的序列同一性，以及
d.具有丁二醇脱氢酶(E.C.1.1.1.4)活性的所述多肽的氨基酸序列具有与SEQ ID NO：114至少80％的序列同一性。
8.根据权利要求1-7中任一项所述的基因修饰的乳酸菌，其中，具有NADH氧化酶活性的所述多肽的氨基酸序列具有与选自以下中的氨基酸序列至少80％的序列同一性：SEQ IDNO：116、118、120、122、124、126、128、130、132、134和136。
9.根据权利要求1至8中任一项所述的基因修饰的乳酸菌，其中，所述乳酸菌的基因组包括编码多肽的基因，所述多肽具有：
k.乳糖特异性磷酸转移酶系统(PTS)活性(EC 2.7.1.69)
l.磷酸-β-D-半乳糖苷酶活性(EC 3.2.1.85)
m.半乳糖-6-磷酸异构酶活性(EC 5.3.1.26)，
n.D-塔格糖-6-磷酸激酶活性(EC 2.7.1.114)，和
o.塔格糖1,6-二磷酸醛缩酶活性(EC 4.1.2.40)。
10.根据权利要求9所述的基因修饰的乳酸菌，其中其中：
a.通过第一种和第二种多肽提供所述乳糖特异性磷酸转移酶系统(PTS)活性(EC2.7.1.69)，其中所述第一种多肽的氨基酸序列具有与SEQ ID NO：210的氨基酸序列至少80％的序列同一性，并且所述第二种多肽的氨基酸序列具有与SEQ ID NO：212的氨基酸序列至少80％的序列同一性；
b.具有磷酸-β-D-半乳糖苷酶活性(EC 3.2.1.85)的所述多肽的氨基酸序列具有与SEQID NO：214的氨基酸序列至少80％的序列同一性；
c.具有半乳糖-6-磷酸异构酶活性(EC 5.3.1.26)的所述多肽的氨基酸序列由第一种和第二种多肽提供，其中所述第一种多肽的氨基酸序列具有与SEQ ID NO：202的氨基酸序列至少80％的序列同一性，并且所述第二种多肽的氨基酸序列具有与SEQ ID NO：204的氨基酸序列至少80％的序列同一性；
d.具有D-塔格糖-6-磷酸激酶活性(EC 2.7.1.114)的所述多肽的氨基酸序列具有与SEQ ID NO：206的氨基酸序列至少80％的序列同一性；以及
e.具有塔格糖1,6-二磷酸醛缩酶活性(EC 4.1.2.40)的所述多肽的氨基酸序列具有与SEQ ID NO：208的氨基酸序列至少80％的序列同一性。
11.一种生产双乙酰的方法，包括以下步骤：
a.将根据权利要求1-10中任一项所述的基因修饰的乳酸菌引入生长培养基以产生培养物，其中所述生长培养基包括原卟啉IX或含铁卟啉的源，
b.在有氧生长条件下培养(a)的所述培养物；
c.回收由所述培养物产生的双乙酰，和可选地
d.分离回收的所述双乙酰。
12.根据权利要求11所述的生产双乙酰的方法，其中，所述含铁卟啉的源是氯化血红素或血红素，其中氯化血红素或血红素的浓度为所述生长培养基的0.3-5μg/ml。
13.根据权利要求11或12所述的生产双乙酰的方法，其中，步骤(a)中所述生长培养基的组合总Fe2+、Fe3+和Cu2+浓度小于20mM。
14.根据权利要求11-13中任一项所述的生产双乙酰的方法，包括附加步骤：步骤(b)中产生的培养物补充有选自由Fe2+、Fe3+和Cu2+组成的组的一种或多种金属离子；并且在步骤(c)之前在有氧条件下孵育所述补充的培养物。
15.根据权利要求11至14中任一项所述的生产双乙酰的方法，其中，所述生长培养基包含乳糖的源，诸如乳清、乳清渗透物或残留乳清渗透物。
16.根据权利要求1-10中任一项所述的基因修饰的乳酸菌用于生产双乙酰或食品的用途。
CN201680071433.3A 2015-10-09 2016-10-07 在代谢工程化的乳酸菌中高水平生产双乙酰 Pending CN108368523A (zh)

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